CsaV3_UNG053440 (gene) Cucumber (Chinese Long) v3 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsaV3_UNG053440
Type	gene
Organism	Cucumis sativus (Cucumber (Chinese Long) v3)
Description	Thymidine kinase
Location	scaffold92 : 61882 .. 64210 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGGCCTCTTTGTCACCGCCCTCTGGCGAGATTCATGTCATTCTGGGGCCGATGTTTGCTGGGAAAACTACCACTCTTCTTCGGCGGATTCAGTCCGAGAGCTGCAATGGCAGGTCTTTTATGCTATGCTTTCTTCTTCTTTAGTTAACCGTTTATTAATTTTCTTTTTATCTTTTAATATATTCTCATCACTCTCTCTGTATTGGCTTGATTATTAGCACGGTTGGTATCGGTATGAAATGGAAAAAAATCGTATTGAATTTACAACTTCATCCGCTATATTACTTGATCGACACTGAATATGAGTGGTTGGGTGGATAAGTTATGGTGAATTTGATATTTTATACTATATCCCTACGAATTTCACAATAATAGAGTTATGCATATTGGTGCTGAATGAGGTTTAAGCTGATATATGATGTTGTGGGGTATGACCGCTTGATAGAGAATGAATGGAACGAGTGAATTGCGAACTAAACTAAACTGATTCTTTGAAGAGAACCTTTAAATCCTTAATTTTTCCTTGCTCCTCGCTCCTCGCTCCCTCTCATTTTTTGAAACAAAAACAAAAAAACGTCTTTATTTTTTAGCTCAACATGGTAGAAGATAGCCTTATTAACGTTAGACCTCATGAACTTGGTTTTTCTTTCAATGAAGACTGCTAATATGGAAACACTGTTGTACCAAGTTTAAAAGAGAATTCTCACCAAGGGGCTGGTACATTTTCCACTCAACCTGTAAAATATAGGAACGTTTAAACAGGTAACATTCATTTACACATGCTTTCTGAAGAGTATGAAGCAATCAATCTACTTATCTGCTATTTGCTGTTGGATCACATAGTTTGGAGATGATACAAGAGTTCAATGACATTTGTGAAATAGACCTTAGAGAGGACATGTTCAAGTCATAGTGGCCCTCTATCTAAAATTTAATACCATCTGAAATTTAATATCATATGAGTTTATTTGACAAGTAAATGAGCAGAGCTACCAGCCAGATTATTGCCCTGTGACAATTGTTGAAGGTGCTCAAGTTAGCTGGAATACTCTCAAGAGAAGAAATTTGTGGAGTTTTGATAGATTTAATATTTCGATGCTCTTCCTGTCCATGTTCACATTCTGGATCTTCGATTAAGCGGTCTTTGTCATCATTTTCATTTGTAGAATCTTTTTTTCTTTGGGTTGGCATCTTGTCTTCCCTATTTTATTATCTTTATATTGAAAATATTTCCTAGCTTTATATTACCTTTTAAAATGTAGAACCTAATTACTGGATGGAATGTGTACTCTGGTGTATCTTAATTGGGTAGAAGCTTCATTTTTGGTACATATCAGAAGGTTTATTCCTTTTCCTTTTCATGCACTTTTTTTACAGAAGTGTAGCTATAATTAAGTCGAATAAAGACACAAGGTATGGATTGGATTCTATTGTTACACATGACGGCATGAAACTCCCTTGCTGGGCAATACCAAACTTATCATCTTTCAAAAAGAAATTTGTCAAGGTTCTCTGACAAGTATATAATCTCTCTCTCTTTATGATGCCAATATCATGCTTAGACATTTTATTAATTAAAATACGAAATATTCAAGCCCTTATAAAAGGTAGCAAAGTATTTAAATCTATGAGGCTAAAAATTTGATTTCCAATTAGAATGAGCAGAAAACTGAATGAGTAGACTTCTGAATTAACCGTAATAATAGGAACTCCTGATTTTCTTGGTAACAGAATTGTTAATTTACATATGATGTTGGGGTGACAGCTAGATGTGATTGGAATTGATGAAGCTCAATTTTTCGACGATCTATATGATTTCTGTTGCGAAGCTGCTGATATTGATGGCAAAACAGTTATAGTTGCTGGGCTGGATGGGGATTACTTGAGGTATAATCAAATTTTCTGGCTTACTTTTGTTTCATACGTTTAATTTCAATGATGTTCTTAGAAACTGATGGCATTTCATATGGTAAAACATTCAATAGAACTTCAGATTGGTTTCTAAATTGACTGATCATTAGTAACTCAATTAAAGCTTTTGTGTTGGGGTTTATACAAAAAACTGAACAGGAGGAACTTCGGTTCAGTTCTCGATATAATTCCTCTTGCCGATTCTGTAACCAAGTTAACTGCTCGATGTGAAATCTGCGGGAATAGGGCTTTCTTTACTTTAAGGAAGACACAAGAAAAAGAGACTGAGCTGATTGGTGGTGCTGATATGTACATGCCCGTATGTCGACAACATTACGTCAGCGGGCAAGTAGCGATAGAGACAGCAAGAACTGTAGTAGAATCACGCAAGGTTGGGTATAGGACTCCAGCATAG

mRNA sequence

ATGAGGGCCTCTTTGTCACCGCCCTCTGGCGAGATTCATGTCATTCTGGGGCCGATGTTTGCTGGGAAAACTACCACTCTTCTTCGGCGGATTCAGTCCGAGAGCTGCAATGGCAGAAGTGTAGCTATAATTAAGTCGAATAAAGACACAAGGTATGGATTGGATTCTATTGTTACACATGACGGCATGAAACTCCCTTGCTGGGCAATACCAAACTTATCATCTTTCAAAAAGAAATTTGTCAAGCTAGATGTGATTGGAATTGATGAAGCTCAATTTTTCGACGATCTATATGATTTCTGTTGCGAAGCTGCTGATATTGATGGCAAAACAGTTATAGTTGCTGGGCTGGATGGGGATTACTTGAGGAGGAACTTCGGTTCAGTTCTCGATATAATTCCTCTTGCCGATTCTGTAACCAAGTTAACTGCTCGATGTGAAATCTGCGGGAATAGGGCTTTCTTTACTTTAAGGAAGACACAAGAAAAAGAGACTGAGCTGATTGGTGGTGCTGATATGTACATGCCCGTATGTCGACAACATTACGTCAGCGGGCAAGTAGCGATAGAGACAGCAAGAACTGTAGTAGAATCACGCAAGGTTGGGTATAGGACTCCAGCATAG

Coding sequence (CDS)

ATGAGGGCCTCTTTGTCACCGCCCTCTGGCGAGATTCATGTCATTCTGGGGCCGATGTTTGCTGGGAAAACTACCACTCTTCTTCGGCGGATTCAGTCCGAGAGCTGCAATGGCAGAAGTGTAGCTATAATTAAGTCGAATAAAGACACAAGGTATGGATTGGATTCTATTGTTACACATGACGGCATGAAACTCCCTTGCTGGGCAATACCAAACTTATCATCTTTCAAAAAGAAATTTGTCAAGCTAGATGTGATTGGAATTGATGAAGCTCAATTTTTCGACGATCTATATGATTTCTGTTGCGAAGCTGCTGATATTGATGGCAAAACAGTTATAGTTGCTGGGCTGGATGGGGATTACTTGAGGAGGAACTTCGGTTCAGTTCTCGATATAATTCCTCTTGCCGATTCTGTAACCAAGTTAACTGCTCGATGTGAAATCTGCGGGAATAGGGCTTTCTTTACTTTAAGGAAGACACAAGAAAAAGAGACTGAGCTGATTGGTGGTGCTGATATGTACATGCCCGTATGTCGACAACATTACGTCAGCGGGCAAGTAGCGATAGAGACAGCAAGAACTGTAGTAGAATCACGCAAGGTTGGGTATAGGACTCCAGCATAG

Protein sequence

MRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTHDGMKLPCWAIPNLSSFKKKFVKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVAGLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYMPVCRQHYVSGQVAIETARTVVESRKVGYRTPA

Homology

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BLAST of CsaV3_UNG053440 vs. NCBI nr
Match: KGN65036.1 (Thymidine kinase [Cucumis sativus])

HSP 1 Score: 415.6 bits (1067), Expect = 9.8e-113
Identity = 206/212 (97.17%), Postives = 206/212 (97.17%), Query Frame = 0


Query: 1   MRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTH 60
           MRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTH
Sbjct: 142 MRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTH 201

Query: 61  DGMKLPCWAIPNLSSFKKKF-----VKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVA 120
           DGMKLPCWAIPNLSSFKKKF      KLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVA
Sbjct: 202 DGMKLPCWAIPNLSSFKKKFGQGSYDKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVA 261

Query: 121 GLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYM 180
           GLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYM
Sbjct: 262 GLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYM 321

Query: 181 PVCRQHYVSGQVAIETARTVVESRKVGYRTPA 208
           PVCRQHYVSGQVAIETARTVVESRKVGYRTPA
Sbjct: 322 PVCRQHYVSGQVAIETARTVVESRKVGYRTPA 353

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BLAST of CsaV3_UNG053440 vs. NCBI nr
Match: XP_011654956.1 (PREDICTED: thymidine kinase [Cucumis sativus])

HSP 1 Score: 415.6 bits (1067), Expect = 9.8e-113
Identity = 206/212 (97.17%), Postives = 206/212 (97.17%), Query Frame = 0


Query: 1   MRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTH 60
           MRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTH
Sbjct: 70  MRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTH 129

Query: 61  DGMKLPCWAIPNLSSFKKKF-----VKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVA 120
           DGMKLPCWAIPNLSSFKKKF      KLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVA
Sbjct: 130 DGMKLPCWAIPNLSSFKKKFGQGSYDKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVA 189

Query: 121 GLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYM 180
           GLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYM
Sbjct: 190 GLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYM 249

Query: 181 PVCRQHYVSGQVAIETARTVVESRKVGYRTPA 208
           PVCRQHYVSGQVAIETARTVVESRKVGYRTPA
Sbjct: 250 PVCRQHYVSGQVAIETARTVVESRKVGYRTPA 281

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BLAST of CsaV3_UNG053440 vs. NCBI nr
Match: XP_016903056.1 (PREDICTED: thymidine kinase a-like [Cucumis melo])

HSP 1 Score: 401.4 bits (1030), Expect = 1.9e-108
Identity = 199/211 (94.31%), Postives = 201/211 (95.26%), Query Frame = 0


Query: 2   RASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTHD 61
           RASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGR+VAIIKSNKDTRYGLDSIVTHD
Sbjct: 71  RASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRNVAIIKSNKDTRYGLDSIVTHD 130

Query: 62  GMKLPCWAIPNLSSFKKKF-----VKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVAG 121
           GMKLPCWAIPNLSSFKKKF      KLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVAG
Sbjct: 131 GMKLPCWAIPNLSSFKKKFGQGSYDKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVAG 190

Query: 122 LDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYMP 181
           LDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGAD+YMP
Sbjct: 191 LDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADVYMP 250

Query: 182 VCRQHYVSGQVAIETARTVVESRKVGYRTPA 208
           VCRQHYVSGQV IE ARTVVESRKV  RTPA
Sbjct: 251 VCRQHYVSGQVVIEAARTVVESRKVECRTPA 281

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BLAST of CsaV3_UNG053440 vs. NCBI nr
Match: XP_022966756.1 (thymidine kinase a-like [Cucurbita maxima])

HSP 1 Score: 375.9 bits (964), Expect = 8.6e-101
Identity = 188/213 (88.26%), Postives = 198/213 (92.96%), Query Frame = 0


Query: 1   MRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTH 60
           M ASLSPPSGE+HVI+GPMFAGKTT+LLRRIQSES NGRSVAIIKSNKDTRYGLDSIVTH
Sbjct: 75  MGASLSPPSGEVHVIVGPMFAGKTTSLLRRIQSESINGRSVAIIKSNKDTRYGLDSIVTH 134

Query: 61  DGMKLPCWAIPNLSSFKKKF-----VKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVA 120
           DGMKLPCWA+PNLSSFK+KF      KLDVIGIDEAQFFDDLYDFC EAAD+DGKTVIVA
Sbjct: 135 DGMKLPCWAVPNLSSFKQKFGEGAYDKLDVIGIDEAQFFDDLYDFCREAADMDGKTVIVA 194

Query: 121 GLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYM 180
           GLDGDYLRR+FGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKT+EKETELIGGADMYM
Sbjct: 195 GLDGDYLRRSFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTEEKETELIGGADMYM 254

Query: 181 PVCRQHYVSGQVAIETARTVV-ESRKVGYRTPA 208
           PVCRQHYVSGQV IETARTVV E+ KV + T A
Sbjct: 255 PVCRQHYVSGQVVIETARTVVEEAHKVEFTTTA 287

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BLAST of CsaV3_UNG053440 vs. NCBI nr
Match: XP_022925302.1 (thymidine kinase a-like [Cucurbita moschata])

HSP 1 Score: 374.0 bits (959), Expect = 3.3e-100
Identity = 186/211 (88.15%), Postives = 197/211 (93.36%), Query Frame = 0


Query: 3   ASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTHDG 62
           ASLSPPSGE+HVI+GPMFAGKTT+LLRRIQSES NGRSVA+IKSNKDTRYGLDSIVTHDG
Sbjct: 77  ASLSPPSGEVHVIVGPMFAGKTTSLLRRIQSESINGRSVAVIKSNKDTRYGLDSIVTHDG 136

Query: 63  MKLPCWAIPNLSSFKKKF-----VKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVAGL 122
           MKLPCWA+PNLSSFK+KF      KLDVIGIDEAQFFDDLYDFC EAAD+DGKTVIVAGL
Sbjct: 137 MKLPCWALPNLSSFKQKFGEGAYDKLDVIGIDEAQFFDDLYDFCREAADMDGKTVIVAGL 196

Query: 123 DGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYMPV 182
           DGDYLRR+FGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKT+EKETELIGGADMYMPV
Sbjct: 197 DGDYLRRSFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTEEKETELIGGADMYMPV 256

Query: 183 CRQHYVSGQVAIETARTVV-ESRKVGYRTPA 208
           CRQHYVSGQV IETARTVV E+ KV + T A
Sbjct: 257 CRQHYVSGQVVIETARTVVEEAHKVEFTTTA 287

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BLAST of CsaV3_UNG053440 vs. TAIR10
Match: AT5G23070.1 (Thymidine kinase)

HSP 1 Score: 296.6 bits (758), Expect = 1.2e-80
Identity = 140/199 (70.35%), Postives = 169/199 (84.92%), Query Frame = 0


Query: 6   SPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTHDGMKL 65
           S   GEIHV++GPMF+GKTTTLLRRI +E   G+ +AIIKSNKDTRY  +SIVTHDG K 
Sbjct: 73  SSSPGEIHVVVGPMFSGKTTTLLRRILAERETGKRIAIIKSNKDTRYCTESIVTHDGEKY 132

Query: 66  PCWAIPNLSSFKKKF------VKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVAGLDG 125
           PCW++P+LSSFK++F       +LDVIGIDEAQFF DLY+FC EAAD +GKTVIVAGLDG
Sbjct: 133 PCWSLPDLSSFKERFGFDDYENRLDVIGIDEAQFFGDLYEFCREAADKEGKTVIVAGLDG 192

Query: 126 DYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYMPVCR 185
           D++RR FGSVLD+IP+AD+VTKLT+RCE+CG RA FT+RKT+EKETELIGGA++YMPVCR
Sbjct: 193 DFMRRRFGSVLDLIPIADTVTKLTSRCEVCGKRALFTMRKTEEKETELIGGAEVYMPVCR 252

Query: 186 QHYVSGQVAIETARTVVES 199
            HYV GQ  +ETAR V++S
Sbjct: 253 SHYVCGQNVLETARAVLDS 271

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BLAST of CsaV3_UNG053440 vs. TAIR10
Match: AT3G07800.1 (Thymidine kinase)

HSP 1 Score: 289.3 bits (739), Expect = 1.9e-78
Identity = 133/194 (68.56%), Postives = 166/194 (85.57%), Query Frame = 0


Query: 9   SGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTHDGMKLPCW 68
           SG +HVI+GPMF+GK+T+LLRRI+SE  +GRSVA++KS+KDTRY  DS+VTHDG+  PCW
Sbjct: 30  SGAVHVIMGPMFSGKSTSLLRRIKSEISDGRSVAMLKSSKDTRYAKDSVVTHDGIGFPCW 89

Query: 69  AIPNLSSFKKKF-----VKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVAGLDGDYLR 128
           A+P+L SF +KF      KLDVIGIDEAQFF DLY+FCC+ AD DGK VIVAGLDGDYLR
Sbjct: 90  ALPDLMSFPEKFGLDAYNKLDVIGIDEAQFFGDLYEFCCKVADDDGKIVIVAGLDGDYLR 149

Query: 129 RNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYMPVCRQHYV 188
           R+FG+VLDIIP+ADSVTKLTARCE+CG++AFFTLRK  +  TELIGGAD+YMPVCR+HY+
Sbjct: 150 RSFGAVLDIIPIADSVTKLTARCEVCGHKAFFTLRKNCDTRTELIGGADVYMPVCRKHYI 209

Query: 189 SGQVAIETARTVVE 198
           +  + I+ ++ V+E
Sbjct: 210 TNHIVIKASKKVLE 223

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BLAST of CsaV3_UNG053440 vs. Swiss-Prot
Match: sp|O81263|KITH_ORYSJ (Thymidine kinase OS=Oryza sativa subsp. japonica OX=39947 GN=TK PE=2 SV=2)

HSP 1 Score: 305.4 bits (781), Expect = 4.7e-82
Identity = 149/206 (72.33%), Postives = 172/206 (83.50%), Query Frame = 0


Query: 1   MRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNG-RSVAIIKSNKDTRYGLDSIVT 60
           M A  S P GEIHVI+GPMFAGKTT LLRR+Q E+  G R+VA+IKS+KD RYGLDS+VT
Sbjct: 59  MEAQPSYP-GEIHVIVGPMFAGKTTALLRRVQVEAGTGSRNVALIKSDKDNRYGLDSVVT 118

Query: 61  HDGMKLPCWAIPNLSSFKKK-----FVKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIV 120
           HDG K+PCWA+P LSSF+ K     + K+DVIGIDEAQFFDDL+DFCC+AAD DGK V+V
Sbjct: 119 HDGTKMPCWALPELSSFQDKLGTEAYDKVDVIGIDEAQFFDDLHDFCCKAADRDGKIVVV 178

Query: 121 AGLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMY 180
           AGLDGDY R  FGSVLDIIPLADSVTKLTARCE+CG RAFFTLRKT+E +TELIGGAD+Y
Sbjct: 179 AGLDGDYKRNKFGSVLDIIPLADSVTKLTARCELCGRRAFFTLRKTRETKTELIGGADVY 238

Query: 181 MPVCRQHYVSGQVAIETARTVVESRK 201
           MPVCRQHY+ GQ+ IE  R V++  K
Sbjct: 239 MPVCRQHYLDGQIVIEATRIVLDLEK 263

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BLAST of CsaV3_UNG053440 vs. Swiss-Prot
Match: sp|F4KBF5|KITHB_ARATH (Thymidine kinase b OS=Arabidopsis thaliana OX=3702 GN=TK1B PE=1 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 2.2e-79
Identity = 140/199 (70.35%), Postives = 169/199 (84.92%), Query Frame = 0


Query: 6   SPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTHDGMKL 65
           S   GEIHV++GPMF+GKTTTLLRRI +E   G+ +AIIKSNKDTRY  +SIVTHDG K 
Sbjct: 73  SSSPGEIHVVVGPMFSGKTTTLLRRILAERETGKRIAIIKSNKDTRYCTESIVTHDGEKY 132

Query: 66  PCWAIPNLSSFKKKF------VKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVAGLDG 125
           PCW++P+LSSFK++F       +LDVIGIDEAQFF DLY+FC EAAD +GKTVIVAGLDG
Sbjct: 133 PCWSLPDLSSFKERFGFDDYENRLDVIGIDEAQFFGDLYEFCREAADKEGKTVIVAGLDG 192

Query: 126 DYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYMPVCR 185
           D++RR FGSVLD+IP+AD+VTKLT+RCE+CG RA FT+RKT+EKETELIGGA++YMPVCR
Sbjct: 193 DFMRRRFGSVLDLIPIADTVTKLTSRCEVCGKRALFTMRKTEEKETELIGGAEVYMPVCR 252

Query: 186 QHYVSGQVAIETARTVVES 199
            HYV GQ  +ETAR V++S
Sbjct: 253 SHYVCGQNVLETARAVLDS 271

[Close]

BLAST of CsaV3_UNG053440 vs. Swiss-Prot
Match: sp|Q9S750|KITHA_ARATH (Thymidine kinase a OS=Arabidopsis thaliana OX=3702 GN=TK1A PE=1 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 3.5e-77
Identity = 133/194 (68.56%), Postives = 166/194 (85.57%), Query Frame = 0


Query: 9   SGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTHDGMKLPCW 68
           SG +HVI+GPMF+GK+T+LLRRI+SE  +GRSVA++KS+KDTRY  DS+VTHDG+  PCW
Sbjct: 30  SGAVHVIMGPMFSGKSTSLLRRIKSEISDGRSVAMLKSSKDTRYAKDSVVTHDGIGFPCW 89

Query: 69  AIPNLSSFKKKF-----VKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVAGLDGDYLR 128
           A+P+L SF +KF      KLDVIGIDEAQFF DLY+FCC+ AD DGK VIVAGLDGDYLR
Sbjct: 90  ALPDLMSFPEKFGLDAYNKLDVIGIDEAQFFGDLYEFCCKVADDDGKIVIVAGLDGDYLR 149

Query: 129 RNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYMPVCRQHYV 188
           R+FG+VLDIIP+ADSVTKLTARCE+CG++AFFTLRK  +  TELIGGAD+YMPVCR+HY+
Sbjct: 150 RSFGAVLDIIPIADSVTKLTARCEVCGHKAFFTLRKNCDTRTELIGGADVYMPVCRKHYI 209

Query: 189 SGQVAIETARTVVE 198
           +  + I+ ++ V+E
Sbjct: 210 TNHIVIKASKKVLE 223

[Close]

BLAST of CsaV3_UNG053440 vs. Swiss-Prot
Match: sp|P23335|KITH_SWPVK (Thymidine kinase OS=Swinepox virus (strain Kasza) OX=10277 GN=TK PE=3 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 8.4e-39
Identity = 80/175 (45.71%), Postives = 113/175 (64.57%), Query Frame = 0


Query: 10  GEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTHDGMKLPCWA 69
           G IH+ILGPMF+GK+T L+R +        +  +IK +KD RYG D++ THD   +   +
Sbjct: 4   GFIHLILGPMFSGKSTELIRLVNRYQIATYNCRVIKYSKDNRYGNDAVYTHDKCYISAVS 63

Query: 70  IPNLSSFKKKFVKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVAGLDGDYLRRNFGSV 129
             +L   K     +D++GIDE QFF+D+ +FC   A+  GK VIVA LDG Y R+ FG++
Sbjct: 64  TDSLFDIKDTLDDVDIVGIDEGQFFNDIVEFCEYIAN-KGKIVIVAALDGTYERKPFGNI 123

Query: 130 LDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYMPVCRQHYVS 185
           L++IPL++ VTKL A C IC   A F+ R + EKE ELIGG + Y+ VCR  Y++
Sbjct: 124 LNLIPLSEKVTKLNAICMICHRDASFSKRLSDEKEIELIGGKEKYLSVCRSCYLT 177

[Close]

BLAST of CsaV3_UNG053440 vs. Swiss-Prot
Match: sp|P04184|KITH_MOUSE (Thymidine kinase, cytosolic OS=Mus musculus OX=10090 GN=Tk1 PE=1 SV=3)

HSP 1 Score: 155.2 bits (391), Expect = 7.8e-37
Identity = 86/180 (47.78%), Postives = 111/180 (61.67%), Query Frame = 0


Query: 3   ASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTHDG 62
           +S S   G+I VILGPMF+GK+T L+RR++          +IK  KDTRY  +S  THD 
Sbjct: 12  SSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYS-NSFSTHDR 71

Query: 63  MKLPCWAIPNLSSFKKKFVKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVAGLDGDYL 122
             +       L    ++ + + VIGIDE QFF D+ DF CE    +GKTVIVA LDG + 
Sbjct: 72  NTMDALPACMLRDVTQESLGVAVIGIDEGQFFPDIVDF-CEMMANEGKTVIVAALDGTFQ 131

Query: 123 RRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYMPVCRQHY 182
           R+ FGS+L+++PLA+SV KLTA C  C   A +T R   EKE E+IGGAD Y  VCR  Y
Sbjct: 132 RKAFGSILNLVPLAESVVKLTAVCMECFREAAYTKRLGLEKEVEVIGGADKYHSVCRLCY 189

[Close]

BLAST of CsaV3_UNG053440 vs. TrEMBL
Match: tr|A0A0A0LYL4|A0A0A0LYL4_CUCSA (Thymidine kinase OS=Cucumis sativus OX=3659 GN=Csa_1G181480 PE=3 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 6.5e-113
Identity = 206/212 (97.17%), Postives = 206/212 (97.17%), Query Frame = 0


Query: 1   MRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTH 60
           MRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTH
Sbjct: 142 MRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTH 201

Query: 61  DGMKLPCWAIPNLSSFKKKF-----VKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVA 120
           DGMKLPCWAIPNLSSFKKKF      KLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVA
Sbjct: 202 DGMKLPCWAIPNLSSFKKKFGQGSYDKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVA 261

Query: 121 GLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYM 180
           GLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYM
Sbjct: 262 GLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYM 321

Query: 181 PVCRQHYVSGQVAIETARTVVESRKVGYRTPA 208
           PVCRQHYVSGQVAIETARTVVESRKVGYRTPA
Sbjct: 322 PVCRQHYVSGQVAIETARTVVESRKVGYRTPA 353

[Close]

BLAST of CsaV3_UNG053440 vs. TrEMBL
Match: tr|A0A1S4E495|A0A1S4E495_CUCME (Thymidine kinase OS=Cucumis melo OX=3656 GN=LOC103501719 PE=3 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 1.3e-108
Identity = 199/211 (94.31%), Postives = 201/211 (95.26%), Query Frame = 0


Query: 2   RASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTHD 61
           RASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGR+VAIIKSNKDTRYGLDSIVTHD
Sbjct: 71  RASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRNVAIIKSNKDTRYGLDSIVTHD 130

Query: 62  GMKLPCWAIPNLSSFKKKF-----VKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVAG 121
           GMKLPCWAIPNLSSFKKKF      KLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVAG
Sbjct: 131 GMKLPCWAIPNLSSFKKKFGQGSYDKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVAG 190

Query: 122 LDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYMP 181
           LDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGAD+YMP
Sbjct: 191 LDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADVYMP 250

Query: 182 VCRQHYVSGQVAIETARTVVESRKVGYRTPA 208
           VCRQHYVSGQV IE ARTVVESRKV  RTPA
Sbjct: 251 VCRQHYVSGQVVIEAARTVVESRKVECRTPA 281

[Close]

BLAST of CsaV3_UNG053440 vs. TrEMBL
Match: tr|A0A2I4F9R8|A0A2I4F9R8_9ROSI (Thymidine kinase OS=Juglans regia OX=51240 GN=LOC108996815 PE=3 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 5.9e-90
Identity = 167/207 (80.68%), Postives = 187/207 (90.34%), Query Frame = 0


Query: 1   MRASLSPPS--GEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIV 60
           M   ++PPS  GEIHVI+GPMF+GKTT+LLRRIQSES NGRSVA+IKSNKDTRYGLD+IV
Sbjct: 68  MHVEVAPPSSTGEIHVIVGPMFSGKTTSLLRRIQSESSNGRSVALIKSNKDTRYGLDAIV 127

Query: 61  THDGMKLPCWAIPNLSSFKKKF-----VKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVI 120
           THDG+KLPCWA+ +LSSF++KF      +LDVIGIDEAQFF+DLYDFC  AAD DGKTVI
Sbjct: 128 THDGVKLPCWALADLSSFRQKFGPDAYDQLDVIGIDEAQFFEDLYDFCRVAADRDGKTVI 187

Query: 121 VAGLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADM 180
           VAGLDGDYLRR+FGSVLDIIPLADSVTKLTARCE+C  RAFFTLRKT+EK+TELIGGAD+
Sbjct: 188 VAGLDGDYLRRSFGSVLDIIPLADSVTKLTARCELCQKRAFFTLRKTEEKQTELIGGADV 247

Query: 181 YMPVCRQHYVSGQVAIETARTVVESRK 201
           YMPVCRQHYVSGQV IE ARTV+ESRK
Sbjct: 248 YMPVCRQHYVSGQVVIEAARTVLESRK 274

[Close]

BLAST of CsaV3_UNG053440 vs. TrEMBL
Match: tr|I1MFP9|I1MFP9_SOYBN (Thymidine kinase OS=Glycine max OX=3847 GN=100809756 PE=3 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 5.0e-89
Identity = 164/200 (82.00%), Postives = 181/200 (90.50%), Query Frame = 0


Query: 7   PPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTHDGMKLP 66
           PPSGEIHVI+GPMFAGKTT+LLRRIQSE+ NGR+VAIIKS+KDTRYGLDSIVTHDG KLP
Sbjct: 74  PPSGEIHVIVGPMFAGKTTSLLRRIQSETANGRNVAIIKSSKDTRYGLDSIVTHDGAKLP 133

Query: 67  CWAIPNLSSFKKKF-----VKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVAGLDGDY 126
           CWA+ NLSSFK+KF      KLDVIGIDEAQFFDDLY+FC +AAD DGKTVIVAGLDG+Y
Sbjct: 134 CWALANLSSFKQKFGMDAYEKLDVIGIDEAQFFDDLYEFCRQAADHDGKTVIVAGLDGNY 193

Query: 127 LRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYMPVCRQH 186
           LRR+FGSVLDIIPLADSVTKLTARCEICG RA FTLRKTQ+K+ ELIGG D+YMPVCRQH
Sbjct: 194 LRRSFGSVLDIIPLADSVTKLTARCEICGKRACFTLRKTQDKQIELIGGVDVYMPVCRQH 253

Query: 187 YVSGQVAIETARTVVESRKV 202
           Y SGQVA+E  R V+ES+KV
Sbjct: 254 YASGQVAMEATRHVLESKKV 273

[Close]

BLAST of CsaV3_UNG053440 vs. TrEMBL
Match: tr|I1L011|I1L011_SOYBN (Thymidine kinase OS=Glycine max OX=3847 GN=100813201 PE=3 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 6.5e-89
Identity = 164/200 (82.00%), Postives = 181/200 (90.50%), Query Frame = 0


Query: 7   PPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSNKDTRYGLDSIVTHDGMKLP 66
           PPSGEIHVI+GPMFAGKTT+LLRRIQSE+ NGR+VAIIKS+KDTRYGLDSIVTHDG +LP
Sbjct: 71  PPSGEIHVIVGPMFAGKTTSLLRRIQSETTNGRNVAIIKSSKDTRYGLDSIVTHDGAELP 130

Query: 67  CWAIPNLSSFKKKF-----VKLDVIGIDEAQFFDDLYDFCCEAADIDGKTVIVAGLDGDY 126
           CWA+ NLSSFK+KF      KLDVIGIDEAQFFDDLY+FC +AAD DGKTVIVAGLDG+Y
Sbjct: 131 CWALENLSSFKQKFGIDAYEKLDVIGIDEAQFFDDLYEFCLQAADHDGKTVIVAGLDGNY 190

Query: 127 LRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETELIGGADMYMPVCRQH 186
           LRR+FGSVLDIIPLADSVTKLTARCEICG RA FTLRKTQ+K+ ELIGG D+YMPVCRQH
Sbjct: 191 LRRSFGSVLDIIPLADSVTKLTARCEICGKRACFTLRKTQDKQIELIGGVDVYMPVCRQH 250

Query: 187 YVSGQVAIETARTVVESRKV 202
           YVSGQVA+E  R V+ES KV
Sbjct: 251 YVSGQVAMEATRHVLESHKV 270

The following BLAST results are available for this feature:

BLAST of CsaV3_UNG053440 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN65036.1	9.8e-113	97.17	Thymidine kinase [Cucumis sativus]	[more]
XP_011654956.1	9.8e-113	97.17	PREDICTED: thymidine kinase [Cucumis sativus]	[more]
XP_016903056.1	1.9e-108	94.31	PREDICTED: thymidine kinase a-like [Cucumis melo]	[more]
XP_022966756.1	8.6e-101	88.26	thymidine kinase a-like [Cucurbita maxima]	[more]
XP_022925302.1	3.3e-100	88.15	thymidine kinase a-like [Cucurbita moschata]	[more]

BLAST of CsaV3_UNG053440 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G23070.1	1.2e-80	70.35	Thymidine kinase	[more]
AT3G07800.1	1.9e-78	68.56	Thymidine kinase	[more]

BLAST of CsaV3_UNG053440 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O81263\|KITH_ORYSJ	4.7e-82	72.33	Thymidine kinase OS=Oryza sativa subsp. japonica OX=39947 GN=TK PE=2 SV=2	[more]
sp\|F4KBF5\|KITHB_ARATH	2.2e-79	70.35	Thymidine kinase b OS=Arabidopsis thaliana OX=3702 GN=TK1B PE=1 SV=1	[more]
sp\|Q9S750\|KITHA_ARATH	3.5e-77	68.56	Thymidine kinase a OS=Arabidopsis thaliana OX=3702 GN=TK1A PE=1 SV=1	[more]
sp\|P23335\|KITH_SWPVK	8.4e-39	45.71	Thymidine kinase OS=Swinepox virus (strain Kasza) OX=10277 GN=TK PE=3 SV=1	[more]
sp\|P04184\|KITH_MOUSE	7.8e-37	47.78	Thymidine kinase, cytosolic OS=Mus musculus OX=10090 GN=Tk1 PE=1 SV=3	[more]

BLAST of CsaV3_UNG053440 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LYL4\|A0A0A0LYL4_CUCSA	6.5e-113	97.17	Thymidine kinase OS=Cucumis sativus OX=3659 GN=Csa_1G181480 PE=3 SV=1	[more]
tr\|A0A1S4E495\|A0A1S4E495_CUCME	1.3e-108	94.31	Thymidine kinase OS=Cucumis melo OX=3656 GN=LOC103501719 PE=3 SV=1	[more]
tr\|A0A2I4F9R8\|A0A2I4F9R8_9ROSI	5.9e-90	80.68	Thymidine kinase OS=Juglans regia OX=51240 GN=LOC108996815 PE=3 SV=1	[more]
tr\|I1MFP9\|I1MFP9_SOYBN	5.0e-89	82.00	Thymidine kinase OS=Glycine max OX=3847 GN=100809756 PE=3 SV=1	[more]
tr\|I1L011\|I1L011_SOYBN	6.5e-89	82.00	Thymidine kinase OS=Glycine max OX=3847 GN=100813201 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004797	thymidine kinase activity
GO:0005524	ATP binding

Vocabulary: **INTERPRO**
Term	Definition
IPR027417	P-loop_NTPase
IPR020633	Thymidine_kinase_CS
IPR001267	Thymidine_kinase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006206	pyrimidine nucleobase metabolic process
biological_process	GO:0006230	TMP biosynthetic process
biological_process	GO:0071897	DNA biosynthetic process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0009157	deoxyribonucleoside monophosphate biosynthetic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004797	thymidine kinase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_UNG053440.1	CsaV3_UNG053440.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001267	Thymidine kinase	PFAM	PF00265	TK	coord: 10..182 e-value: 9.8E-55 score: 185.0
IPR001267	Thymidine kinase	PIRSF	PIRSF035805	Tdk	coord: 8..195 e-value: 5.6E-63 score: 209.8
IPR001267	Thymidine kinase	PANTHER	PTHR11441	THYMIDINE KINASE	coord: 4..201
None	No IPR available	GENE3D	G3DSA:3.40.50.300		coord: 9..142 e-value: 3.7E-51 score: 174.6
None	No IPR available	GENE3D	G3DSA:3.30.60.20		coord: 143..186 e-value: 9.0E-19 score: 69.2
None	No IPR available	PANTHER	PTHR11441:SF2	THYMIDINE KINASE B	coord: 4..201
None	No IPR available	SUPERFAMILY	SSF57716	Glucocorticoid receptor-like (DNA-binding domain)	coord: 143..187
IPR020633	Thymidine kinase, conserved site	PROSITE	PS00603	TK_CELLULAR_TYPE	coord: 169..182
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	SSF52540	P-loop containing nucleoside triphosphate hydrolases	coord: 10..143

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsaV3_UNG053440	CSPI01G15760	Wild cucumber (PI 183967)	cpicucB060
CsaV3_UNG053440	Cucsa.180490	Cucumber (Gy14) v1	cgycucB291
CsaV3_UNG053440	CmaCh18G006010	Cucurbita maxima (Rimu)	cmacucB0510
CsaV3_UNG053440	CmoCh18G005930	Cucurbita moschata (Rifu)	cmocucB0496
CsaV3_UNG053440	Cp4.1LG09g06410	Cucurbita pepo (Zucchini)	cpecucB0056
CsaV3_UNG053440	CsGy1G015460	Cucumber (Gy14) v2	cgybcucB051
CsaV3_UNG053440	Carg25612	Silver-seed gourd	carcucB0057
CsaV3_UNG053440	Cla009043	Watermelon (97103) v1	cucwmB575
CsaV3_UNG053440	MELO3C025541	Melon (DHL92) v3.5.1	cucmeB527
CsaV3_UNG053440	ClCG01G010620	Watermelon (Charleston Gray)	cucwcgB534
CsaV3_UNG053440	Lsi02G011250	Bottle gourd (USVL1VR-Ls)	cuclsiB518
CsaV3_UNG053440	MELO3C025541.2	Melon (DHL92) v3.6.1	cucmedB514
CsaV3_UNG053440	Cla97C01G010410	Watermelon (97103) v2	cucwmbB533
CsaV3_UNG053440	Bhi08G000630	Wax gourd	cucwgoB671

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CsaV3_UNG053440	CsaV3_1G017040	Cucumber (Chinese Long) v3	cuccucB056

The following block(s) are covering this gene:


Gene	Organism	Block
CsaV3_UNG053440	Silver-seed gourd	carcucB1085
CsaV3_UNG053440	Cucurbita maxima (Rimu)	cmacucB0268
CsaV3_UNG053440	Cucurbita moschata (Rifu)	cmocucB0258
CsaV3_UNG053440	Cucurbita pepo (Zucchini)	cpecucB0655