Csa1G108280 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa1G108280
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Iron--sulfur cluster insertion protein ErpA; contains IPR000361 (FeS cluster biogenesis), IPR016092 (FeS cluster insertion protein)
Location	Chr1 : 8742411 .. 8744973 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTCCCAGATTTCTAATTCAGCGGCTAACACCATATTTGGGAGCACATATCCGAAAGAACCAAAGGCTTCTTAGCTCTTCTGTTTCAGCCTATCAAGAAGCCTCTTCTTCGTTGATAACTCCATCTGCGGGCATTGATACAATTCACATGTCAGATAACTGCATTCGGGTATCCCTACTCTTTATGTAATCCTTTTTGTGCATTTACCATATGGATTTAAAATTATTCATCTATTTCACTGTCATTATTGTTTTAGTTTTTGATTTTCTGGACTATCCTCATTTTGTGCATTATTTGGACCTATAATTTCTTGGTTGTAGGATGTTAATGAACTATTGCCTTGAACACGATTAAAATATGAGTGAAGAATTTGAGATTTGGGCATTTTCTTGTTATCGCTTCAAATTTGGGCATTTGCTTTGTCATACGTGATGCCCTAAAGCGAGGCTTAATTTATGATGCCAAAACTGAAGTATTAAGAAGAATATATGGTGCCAGTTATAATAATTATCTTATTCTTAATTTTTTAAAGAACTTTAAATAAATGAAAGGAAGTGAGACTTATAAACTTAGGAATGAACTCTTCACAACTAGATGTATGATGTATTATCTGTTACCAAAGGGCTAAATTAGTTAATACTCGCAATTATGTTATTCATCACGGTTGACATAGTCCTTGATCTTCATCCTTTAAAAGCATTCTTACCATCATTTTTCTACACATTTTTTTGCCTGCTGCACTAGTGTAAGTTTTGAATAATGCTGAGTATTGAGATGCCCTTGCAGAGAATGAAAGAATTGCAAGAACCCATGGAAGAAAAGATGCTTCGGTTGAGTGTAGAAAATGGGGGATGTTCTGGGTTTCAATATGTTTTCAATTTGGATGACAAAACCAATCCAGATGACAGGTCTATTAAATAGCTTATACTCACTTCTTAATTTGGATTTTCACCTAAAGATTGCCAATGATGTTTGCTTTCACTCTTTAAAACTTCATCCTTTTCCCCCCTTTCATGTTTGCAGGGTGTGTGAGAAGGAAGGAGTTAAATTGGTAGTTGATAACATCTCATATGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAGGAGCTGATTCGTTCCGCTTTCATTGTGAGTAAACCTATGTCTCACCATTTGCTGGATCACCATTGTTGCTTAGATATGTTTATTTTAGTTTCAAAATGTTACACTTTTAGTTAATAAGTTTTCCTTCGATTTTAATTTAGCCTTTAGATTTCAAAATATTATGCTTTTAGACCTTAAAATTTGAATTTTATTTTAATCTAGTAACAAGTTTCAAGATTTACACTTTTTAACCTCAATTTTTAAATAACATATTCACTCCAATCTTTATCTTTAATATCTAAAATTAATTATGAAGTGAAGTTCCAAAATTAACTTCAACATTGATGAAGAAATAATGAAACATAATCCATTATAGTTTTCTTAATTATTTTTACTTAGTCTTTAAAAGTGAGTATTGATTTAAAAGTGAGTATTGAGTGAAAATTGAGATTGAAAATGTAAATCTTGAGTTTAGGGACCAAATTGAAACAAAACTCAAACACTTATATCAAACGGTAACATTTTGAAATCTAAGGATTAAATTGAAATCAAACTCAAAACTTAATCATTAAAGCTTTTGTCCATTTAGTTTCTTTTTTAAAAATCAAGTTTATTAACATTGCTTCCTTTTATTACTTTCCATGCTTTGTTGTCTCAAAATCATAGCCAAAATCTGAAAACTAAAAAGGTAAATTTCAAACGCTTGTTTTTATTTCTAGAAATTGACTAAAGAACTTTATGGGCGTTTGGGACAAAGTGACCACAAATCCAAGTGATGTTGAGGGAGGTTATGGTTCCTAAAGTACTTCTAAATTTTGCTATAACATATAAATAGTTTAGTCTATTTTTTTATACGTAAAATGCTCCAAACAAAAAAAAACTAAATAGTTACTAAACGACATTTAAAAGGGTAATATTTTGAAATCTAAAAGTTGAACCGAAACTAAATCTAAAACCTGGAGACCAAAAAACACATCTCATTTTACATCATATATTTTATGCATTCTTATAAATCCAAAACTTGGAGACCAAAGAACATATATATCTCATTTTACATCATATATTTGATCCATTCTTATTTCTTATTTCACAACAAAAGTAGTGCTAATGCAGAAGTTGAATTCTTGTAATATGCCATATGCAGGTGACCACAAATCCAAGTGCTGTTGGTGGCTGTAGTTGTAAAAGTTCTTTTATGGTGAAACAATAATCATCACAAAGCTCTTCTCAGGAAGGCATAGAAGCAATAACCAAAACTCTGACTTGAAGATATAATGGATTTGTTTATCACTTGGTTGGTTCAGTTGATAAGGCTTTTTGTCCATTCCGATAATTTTTGTATCATGTGCCGCTTTTATATTACTTGAGAACTTCATACTTCATTCTTTGTTGCACTTAATCATGATGGATTTTATTACAAGAGAGTTGCATTGGATAATAAAATATACTTGTATATTTGCAAGTCTTTTAAAA

mRNA sequence

ATGGTTCCCAGATTTCTAATTCAGCGGCTAACACCATATTTGGGAGCACATATCCGAAAGAACCAAAGGCTTCTTAGCTCTTCTGTTTCAGCCTATCAAGAAGCCTCTTCTTCGTTGATAACTCCATCTGCGGGCATTGATACAATTCACATGTCAGATAACTGCATTCGGAGAATGAAAGAATTGCAAGAACCCATGGAAGAAAAGATGCTTCGGTTGAGTGTAGAAAATGGGGGATGTTCTGGGTTTCAATATGTTTTCAATTTGGATGACAAAACCAATCCAGATGACAGGGTGTGTGAGAAGGAAGGAGTTAAATTGGTAGTTGATAACATCTCATATGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAGGAGCTGATTCGTTCCGCTTTCATTGTGACCACAAATCCAAGTGCTGTTGGTGGCTGTAGTTGTAAAAGTTCTTTTATGGTGAAACAATAA

Coding sequence (CDS)

ATGGTTCCCAGATTTCTAATTCAGCGGCTAACACCATATTTGGGAGCACATATCCGAAAGAACCAAAGGCTTCTTAGCTCTTCTGTTTCAGCCTATCAAGAAGCCTCTTCTTCGTTGATAACTCCATCTGCGGGCATTGATACAATTCACATGTCAGATAACTGCATTCGGAGAATGAAAGAATTGCAAGAACCCATGGAAGAAAAGATGCTTCGGTTGAGTGTAGAAAATGGGGGATGTTCTGGGTTTCAATATGTTTTCAATTTGGATGACAAAACCAATCCAGATGACAGGGTGTGTGAGAAGGAAGGAGTTAAATTGGTAGTTGATAACATCTCATATGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAGGAGCTGATTCGTTCCGCTTTCATTGTGACCACAAATCCAAGTGCTGTTGGTGGCTGTAGTTGTAAAAGTTCTTTTATGGTGAAACAATAA

Protein sequence

MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ*

Homology

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BLAST of Csa1G108280 vs. Swiss-Prot
Match: ISAM2_ARATH (Iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Arabidopsis thaliana GN=At5g03905 PE=2 SV=2)

HSP 1 Score: 170.2 bits (430), Expect = 1.6e-41
Identity = 92/136 (67.65%), Postives = 107/136 (78.68%), Query Frame = 1


Query: 4   RFLIQRLTPYLGAHIRKNQRLLS-SSVSAYQEASSSLITP--SAGIDTIHMSDNCIRRMK 63
           R L++R+ PYL   IR+N RLL+ SS SA +EASSS  +   S+  D +H+SDNCIRRMK
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASSSSSSQPESSSNDVVHLSDNCIRRMK 62

Query: 64  ELQ--EPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVK 123
           ELQ  EP E+KMLRL VE GGCSGFQY F LD++TNPDDRV EK GVKLVVDN+SYD VK
Sbjct: 63  ELQSSEP-EKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYDLVK 122

Query: 124 GATIDYVEELIRSAFI 135
           GATIDY EELIR+AF+
Sbjct: 123 GATIDYEEELIRAAFV 137

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BLAST of Csa1G108280 vs. Swiss-Prot
Match: ISCA2_BOVIN (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Bos taurus GN=ISCA2 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 5.6e-23
Identity = 57/114 (50.00%), Postives = 74/114 (64.91%), Query Frame = 1


Query: 20  KNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMKELQEPMEEKMLRLSVENGG 79
           +  RLL++S         S  +P AG   IH++D+C++R+ E+ E  E   LRL VE GG
Sbjct: 19  QRSRLLAASRGPRALREVSFSSPEAGEGQIHLTDSCVQRLLEITEGSE--FLRLEVEGGG 78

Query: 80  CSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELIRSAF 134
           CSGFQY F+LD   NPDDRV EK G ++VVD+ S  FVKGA +D+ +ELIRS+F
Sbjct: 79  CSGFQYKFSLDTVINPDDRVFEKGGARVVVDSDSLAFVKGAHVDFSQELIRSSF 130

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BLAST of Csa1G108280 vs. Swiss-Prot
Match: ISCA2_HUMAN (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Homo sapiens GN=ISCA2 PE=1 SV=2)

HSP 1 Score: 107.8 bits (268), Expect = 9.5e-23
Identity = 60/113 (53.10%), Postives = 79/113 (69.91%), Query Frame = 1


Query: 23  RLLSSSVS--AYQEASSSLITPSAGIDTIHMSDNCIRRMKELQEPMEEKMLRLSVENGGC 82
           RLL++S+   A +EASSS  +P AG   I ++D+C++R+ E+ E  E   LRL VE GGC
Sbjct: 24  RLLTASLGPQARREASSS--SPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGGGC 83

Query: 83  SGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELIRSAF 134
           SGFQY F+LD   NPDDRV E+ G ++VVD+ S  FVKGA +D+ +ELIRS+F
Sbjct: 84  SGFQYKFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSF 132

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BLAST of Csa1G108280 vs. Swiss-Prot
Match: ISCA2_PONAB (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Pongo abelii GN=ISCA2 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 9.5e-23
Identity = 60/113 (53.10%), Postives = 79/113 (69.91%), Query Frame = 1


Query: 23  RLLSSSVS--AYQEASSSLITPSAGIDTIHMSDNCIRRMKELQEPMEEKMLRLSVENGGC 82
           RLL++S+   A +EASSS  +P AG   I ++D+C++R+ E+ E  E   LRL VE GGC
Sbjct: 24  RLLAASLGPQARREASSS--SPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGGGC 83

Query: 83  SGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELIRSAF 134
           SGFQY F+LD   NPDDRV E+ G ++VVD+ S  FVKGA +D+ +ELIRS+F
Sbjct: 84  SGFQYKFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSF 132

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BLAST of Csa1G108280 vs. Swiss-Prot
Match: ISCA2_MOUSE (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Mus musculus GN=Isca2 PE=1 SV=2)

HSP 1 Score: 102.1 bits (253), Expect = 5.2e-21
Identity = 54/115 (46.96%), Postives = 73/115 (63.48%), Query Frame = 1


Query: 19  RKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMKELQEPMEEKMLRLSVENG 78
           R++ RLL+          ++   P AG   I ++D+C++R+ E+ E  E   LRL VE G
Sbjct: 20  RRSGRLLAVFPRLLTRWETTSSIPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGG 79

Query: 79  GCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELIRSAF 134
           GCSGFQY F+LD   NPDDRV E+ G ++VVD+ S  FVKGA +D+ +ELIRS+F
Sbjct: 80  GCSGFQYKFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSF 132

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BLAST of Csa1G108280 vs. TrEMBL
Match: A0A0A0LSN2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G108280 PE=4 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 4.5e-72
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 1


Query: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60
           MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK
Sbjct: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60

Query: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120
           ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA
Sbjct: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120

Query: 121 TIDYVEELIRSAFIPKSEN 140
           TIDYVEELIRSAFIPKSEN
Sbjct: 121 TIDYVEELIRSAFIPKSEN 139

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BLAST of Csa1G108280 vs. TrEMBL
Match: A0A0A0LXX8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G496290 PE=4 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 8.0e-53
Identity = 110/136 (80.88%), Postives = 120/136 (88.24%), Query Frame = 1


Query: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEAS--SSLITPSAGIDTIHMSDNCIRR 60
           M PR LIQR+TPYL A I++NQRLLSSS SAYQEA   S   +PSA +DTIHM+D+CIRR
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRR 60

Query: 61  MKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVK 120
           MKELQ+P EEKMLRLSVE GGCSGFQYVFNLD KTNPDDR+ EKEGVKLVVDNISYDFVK
Sbjct: 61  MKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK 120

Query: 121 GATIDYVEELIRSAFI 135
           GATIDYVEELIRSAF+
Sbjct: 121 GATIDYVEELIRSAFV 136

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BLAST of Csa1G108280 vs. TrEMBL
Match: A0A140B491_PRUPE (Fe-S cluster assembly transfer protein 19 OS=Prunus persica var. persica GN=ISA3 PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.3e-42
Identity = 97/138 (70.29%), Postives = 111/138 (80.43%), Query Frame = 1


Query: 1   MVPRFLI-QRLTPYLGAHIRKNQRLLSSSV---SAYQEASSSLITPSAGIDTIHMSDNCI 60
           M PR LI  RLTP+  A IR+NQRLLSSS    SA +EASSS  + S  +D + M+D C+
Sbjct: 1   MAPRSLILHRLTPFFAARIRQNQRLLSSSFYSSSAVEEASSSSSSSSPSLDAVVMTDTCV 60

Query: 61  RRMKELQ-EPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYD 120
           +RMK+LQ    E KMLRLSVE GGCSGFQYVF+LDDKT+PDDRV EKEGVKLVVDN+SYD
Sbjct: 61  QRMKQLQASEAEGKMLRLSVETGGCSGFQYVFDLDDKTHPDDRVFEKEGVKLVVDNVSYD 120

Query: 121 FVKGATIDYVEELIRSAF 134
           FVKGAT+DYVEELIRSAF
Sbjct: 121 FVKGATVDYVEELIRSAF 138

[Close]

BLAST of Csa1G108280 vs. TrEMBL
Match: M5WAY8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012679mg PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.3e-42
Identity = 97/138 (70.29%), Postives = 111/138 (80.43%), Query Frame = 1


Query: 1   MVPRFLI-QRLTPYLGAHIRKNQRLLSSSV---SAYQEASSSLITPSAGIDTIHMSDNCI 60
           M PR LI  RLTP+  A IR+NQRLLSSS    SA +EASSS  + S  +D + M+D C+
Sbjct: 1   MAPRSLILHRLTPFFAARIRQNQRLLSSSFYSSSAVEEASSSSSSSSPSLDAVVMTDTCV 60

Query: 61  RRMKELQ-EPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYD 120
           +RMK+LQ    E KMLRLSVE GGCSGFQYVF+LDDKT+PDDRV EKEGVKLVVDN+SYD
Sbjct: 61  QRMKQLQASEAEGKMLRLSVETGGCSGFQYVFDLDDKTHPDDRVFEKEGVKLVVDNVSYD 120

Query: 121 FVKGATIDYVEELIRSAF 134
           FVKGAT+DYVEELIRSAF
Sbjct: 121 FVKGATVDYVEELIRSAF 138

[Close]

BLAST of Csa1G108280 vs. TrEMBL
Match: A0A0D2QDP8_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_002G158400 PE=4 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.1e-41
Identity = 94/137 (68.61%), Postives = 111/137 (81.02%), Query Frame = 1


Query: 4   RFLIQRLTPYLGAHIRKNQRLLSSSVS----AYQEASSSLITPSAGIDTIHMSDNCIRRM 63
           R LIQR++P+  A I++N +LLSSS S    A  EASS   +PS  +D +HM+DNCI+RM
Sbjct: 11  RSLIQRISPFFIARIKQNHKLLSSSFSSSSSALHEASSE--SPSPSLDAVHMTDNCIKRM 70

Query: 64  KELQ--EPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFV 123
           KELQ  E   ++MLRLSVE GGCSGFQYVF+LDDKTNPDDRV  +EGVKLVVD+ISYDFV
Sbjct: 71  KELQASEQSSDQMLRLSVETGGCSGFQYVFDLDDKTNPDDRVFVREGVKLVVDDISYDFV 130

Query: 124 KGATIDYVEELIRSAFI 135
           KGATIDYVEELIRSAF+
Sbjct: 131 KGATIDYVEELIRSAFV 145

[Close]

BLAST of Csa1G108280 vs. TAIR10
Match: AT5G03905.1 (AT5G03905.1 Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 170.2 bits (430), Expect = 8.8e-43
Identity = 92/136 (67.65%), Postives = 107/136 (78.68%), Query Frame = 1


Query: 4   RFLIQRLTPYLGAHIRKNQRLLS-SSVSAYQEASSSLITP--SAGIDTIHMSDNCIRRMK 63
           R L++R+ PYL   IR+N RLL+ SS SA +EASSS  +   S+  D +H+SDNCIRRMK
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASSSSSSQPESSSNDVVHLSDNCIRRMK 62

Query: 64  ELQ--EPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVK 123
           ELQ  EP E+KMLRL VE GGCSGFQY F LD++TNPDDRV EK GVKLVVDN+SYD VK
Sbjct: 63  ELQSSEP-EKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYDLVK 122

Query: 124 GATIDYVEELIRSAFI 135
           GATIDY EELIR+AF+
Sbjct: 123 GATIDYEEELIRAAFV 137

[Close]

BLAST of Csa1G108280 vs. TAIR10
Match: AT2G36260.1 (AT2G36260.1 Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 62.8 bits (151), Expect = 2.0e-10
Identity = 30/84 (35.71%), Postives = 49/84 (58.33%), Query Frame = 1


Query: 51  MSDNCIRRMKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVD 110
           +SD    R+++L +  ++  LRL+VE  GC+G  YV N   +    D V E++GVK++VD
Sbjct: 8   LSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKGVKILVD 67

Query: 111 NISYDFVKGATIDYVEELIRSAFI 135
             +   V G  +D+V++ +RS F+
Sbjct: 68  PKAVMHVIGTEMDFVDDKLRSEFV 91

[Close]

BLAST of Csa1G108280 vs. TAIR10
Match: AT1G10500.1 (AT1G10500.1 chloroplast-localized ISCA-like protein)

HSP 1 Score: 60.1 bits (144), Expect = 1.3e-09
Identity = 36/124 (29.03%), Postives = 61/124 (49.19%), Query Frame = 1


Query: 13  YLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMKELQ-EPMEEKML 72
           Y+  ++ +  RL   S S     +   + P+     I +S+N ++ + +++ E  E+  L
Sbjct: 41  YVNLNLNRRNRLSVRSASVPAAPAMEGLKPA-----ISLSENALKHLSKMRSERGEDLCL 100

Query: 73  RLSVENGGCSGFQYVFNLDDKTN--PDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELI 132
           R+ V+ GGCSG  Y  + +++ N  PDD   E +G  +V D  S  F+ G  +DY + LI
Sbjct: 101 RIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYSDALI 159

Query: 133 RSAF 134
              F
Sbjct: 161 GGGF 159

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BLAST of Csa1G108280 vs. NCBI nr
Match: gi|700209707|gb|KGN64803.1| (hypothetical protein Csa_1G108280 [Cucumis sativus])

HSP 1 Score: 278.5 bits (711), Expect = 6.5e-72
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 1


Query: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60
           MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK
Sbjct: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60

Query: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120
           ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA
Sbjct: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120

Query: 121 TIDYVEELIRSAFIPKSEN 140
           TIDYVEELIRSAFIPKSEN
Sbjct: 121 TIDYVEELIRSAFIPKSEN 139

[Close]

BLAST of Csa1G108280 vs. NCBI nr
Match: gi|449459108|ref|XP_004147288.1| (PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X1 [Cucumis sativus])

HSP 1 Score: 268.1 bits (684), Expect = 8.8e-69
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 1


Query: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60
           MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK
Sbjct: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60

Query: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120
           ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA
Sbjct: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120

Query: 121 TIDYVEELIRSAFI 135
           TIDYVEELIRSAFI
Sbjct: 121 TIDYVEELIRSAFI 134

[Close]

BLAST of Csa1G108280 vs. NCBI nr
Match: gi|778659089|ref|XP_011653808.1| (PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X2 [Cucumis sativus])

HSP 1 Score: 268.1 bits (684), Expect = 8.8e-69
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 1


Query: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60
           MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK
Sbjct: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60

Query: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120
           ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA
Sbjct: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120

Query: 121 TIDYVEELIRSAFI 135
           TIDYVEELIRSAFI
Sbjct: 121 TIDYVEELIRSAFI 134

[Close]

BLAST of Csa1G108280 vs. NCBI nr
Match: gi|449470384|ref|XP_004152897.1| (PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucumis sativus])

HSP 1 Score: 214.5 bits (545), Expect = 1.2e-52
Identity = 110/136 (80.88%), Postives = 120/136 (88.24%), Query Frame = 1


Query: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEAS--SSLITPSAGIDTIHMSDNCIRR 60
           M PR LIQR+TPYL A I++NQRLLSSS SAYQEA   S   +PSA +DTIHM+D+CIRR
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRR 60

Query: 61  MKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVK 120
           MKELQ+P EEKMLRLSVE GGCSGFQYVFNLD KTNPDDR+ EKEGVKLVVDNISYDFVK
Sbjct: 61  MKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK 120

Query: 121 GATIDYVEELIRSAFI 135
           GATIDYVEELIRSAF+
Sbjct: 121 GATIDYVEELIRSAFV 136

[Close]

BLAST of Csa1G108280 vs. NCBI nr
Match: gi|659115367|ref|XP_008457521.1| (PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 212.6 bits (540), Expect = 4.4e-52
Identity = 109/138 (78.99%), Postives = 120/138 (86.96%), Query Frame = 1


Query: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPS----AGIDTIHMSDNCI 60
           M PR LIQR+TPYL A I++NQRLLSSS +AYQEASS   +PS    A +DTIHM+D+CI
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSATAYQEASSLSSSPSPSPSAPVDTIHMTDSCI 60

Query: 61  RRMKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDF 120
           RRMKELQ+P EEKMLRLSVE GGCSGFQYVFNLD KT PDDR+ EKEGVKLVVDNISYDF
Sbjct: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTTPDDRIYEKEGVKLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFI 135
           VKGATIDYVEELIRSAF+
Sbjct: 121 VKGATIDYVEELIRSAFV 138

The following BLAST results are available for this feature:

BLAST of Csa1G108280 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ISAM2_ARATH	1.6e-41	67.65	Iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Arabidopsis thaliana ...	[more]
ISCA2_BOVIN	5.6e-23	50.00	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Bos taurus GN=ISCA2 PE=...	[more]
ISCA2_HUMAN	9.5e-23	53.10	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Homo sapiens GN=ISCA2 P...	[more]
ISCA2_PONAB	9.5e-23	53.10	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Pongo abelii GN=ISCA2 P...	[more]
ISCA2_MOUSE	5.2e-21	46.96	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Mus musculus GN=Isca2 P...	[more]

BLAST of Csa1G108280 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LSN2_CUCSA	4.5e-72	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G108280 PE=4 SV=1	[more]
A0A0A0LXX8_CUCSA	8.0e-53	80.88	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G496290 PE=4 SV=1	[more]
A0A140B491_PRUPE	1.3e-42	70.29	Fe-S cluster assembly transfer protein 19 OS=Prunus persica var. persica GN=ISA3...	[more]
M5WAY8_PRUPE	1.3e-42	70.29	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012679mg PE=4 SV=1	[more]
A0A0D2QDP8_GOSRA	1.1e-41	68.61	Uncharacterized protein OS=Gossypium raimondii GN=B456_002G158400 PE=4 SV=1	[more]

BLAST of Csa1G108280 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G03905.1	8.8e-43	67.65	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G36260.1	2.0e-10	35.71	Iron-sulphur cluster biosynthesis family protein	[more]
AT1G10500.1	1.3e-09	29.03	chloroplast-localized ISCA-like protein	[more]

BLAST of Csa1G108280 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700209707\|gb\|KGN64803.1\|	6.5e-72	100.00	hypothetical protein Csa_1G108280 [Cucumis sativus]	[more]
gi\|449459108\|ref\|XP_004147288.1\|	8.8e-69	100.00	PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X1 [C...	[more]
gi\|778659089\|ref\|XP_011653808.1\|	8.8e-69	100.00	PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X2 [C...	[more]
gi\|449470384\|ref\|XP_004152897.1\|	1.2e-52	80.88	PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucumis sati...	[more]
gi\|659115367\|ref\|XP_008457521.1\|	4.4e-52	78.99	PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X2 [C...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000361	FeS_biogenesis
IPR016092	FeS_cluster_insertion
IPR000361	FeS_biogenesis
IPR016092	FeS_cluster_insertion

Vocabulary: **Molecular Function**
Term	Definition
GO:0005198	structural molecule activity
GO:0051536	iron-sulfur cluster binding
GO:0005198	structural molecule activity
GO:0051536	iron-sulfur cluster binding

Vocabulary: **Biological Process**
Term	Definition
GO:0016226	iron-sulfur cluster assembly
GO:0016226	iron-sulfur cluster assembly

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0097428	protein maturation by iron-sulfur cluster transfer
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0005198	structural molecule activity

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
CU102444	cucumber EST collection version 3.0	transcribed_cluster
CU116498	cucumber EST collection version 3.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa1G108280.2	Csa1G108280.2	mRNA
Csa1G108280.1	Csa1G108280.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
CU102444	CU102444	transcribed_cluster
CU116498	CU116498	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000361	FeS cluster biogenesis	GENE3D	G3DSA:2.60.300.12		coord: 48..139 score: 5.5
IPR000361	FeS cluster biogenesis	PFAM	PF01521	Fe-S_biosyn	coord: 49..148 score: 3.9
IPR000361	FeS cluster biogenesis	unknown	SSF89360	HesB-like domain	coord: 48..141 score: 1.96
IPR016092	FeS cluster insertion protein	TIGRFAMs	TIGR00049	TIGR00049	coord: 49..153 score: 2.0
None	No IPR available	PANTHER	PTHR10072	IRON-SULFUR CLUSTER ASSEMBLY PROTEIN	coord: 3..154 score: 3.5
None	No IPR available	PANTHER	PTHR10072:SF46	IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL	coord: 3..154 score: 3.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa1G108280	Cla005089	Watermelon (97103) v1	cuwmB010
Csa1G108280	ClCG03G002740	Watermelon (Charleston Gray)	cuwcgB037
Csa1G108280	Cla97C03G053770	Watermelon (97103) v2	cuwmbB034
Csa1G108280	Bhi08G000209	Wax gourd	cuwgoB049
Csa1G108280	CSPI01G13300	Wild cucumber (PI 183967)	cpicuB000
Csa1G108280	Cucsa.292560	Cucumber (Gy14) v1	cgycuB419
Csa1G108280	Cp4.1LG02g12660	Cucurbita pepo (Zucchini)	cpecuB528
Csa1G108280	CsGy1G013410	Cucumber (Gy14) v2	cgybcuB001
Csa1G108280	Carg12293	Silver-seed gourd	carcuB0472

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Csa1G108280	Wax gourd	cuwgoB016
Csa1G108280	Cucumber (Chinese Long) v2	cucuB018
Csa1G108280	Cucurbita maxima (Rimu)	cmacuB203
Csa1G108280	Cucurbita maxima (Rimu)	cmacuB431
Csa1G108280	Cucurbita moschata (Rifu)	cmocuB188
Csa1G108280	Cucurbita moschata (Rifu)	cmocuB421
Csa1G108280	Melon (DHL92) v3.5.1	cumeB015
Csa1G108280	Cucurbita pepo (Zucchini)	cpecuB516
Csa1G108280	Bottle gourd (USVL1VR-Ls)	culsiB044
Csa1G108280	Cucumber (Gy14) v2	cgybcuB097
Csa1G108280	Melon (DHL92) v3.6.1	cumedB010
Csa1G108280	Silver-seed gourd	carcuB0360
Csa1G108280	Silver-seed gourd	carcuB1083