Csa1G108280.2 (mRNA) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa1G108280.2
Type	mRNA
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Iron--sulfur cluster insertion protein ErpA; contains IPR000361 (FeS cluster biogenesis), IPR016092 (FeS cluster insertion protein)
Location	Chr1 : 8742411 .. 8744973 (-)
Sequence length	468

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTCCCAGATTTCTAATTCAGCGGCTAACACCATATTTGGGAGCACATATCCGAAAGAACCAAAGGCTTCTTAGCTCTTCTGTTTCAGCCTATCAAGAAGCCTCTTCTTCGTTGATAACTCCATCTGCGGGCATTGATACAATTCACATGTCAGATAACTGCATTCGGGTATCCCTACTCTTTATGTAATCCTTTTTGTGCATTTACCATATGGATTTAAAATTATTCATCTATTTCACTGTCATTATTGTTTTAGTTTTTGATTTTCTGGACTATCCTCATTTTGTGCATTATTTGGACCTATAATTTCTTGGTTGTAGGATGTTAATGAACTATTGCCTTGAACACGATTAAAATATGAGTGAAGAATTTGAGATTTGGGCATTTTCTTGTTATCGCTTCAAATTTGGGCATTTGCTTTGTCATACGTGATGCCCTAAAGCGAGGCTTAATTTATGATGCCAAAACTGAAGTATTAAGAAGAATATATGGTGCCAGTTATAATAATTATCTTATTCTTAATTTTTTAAAGAACTTTAAATAAATGAAAGGAAGTGAGACTTATAAACTTAGGAATGAACTCTTCACAACTAGATGTATGATGTATTATCTGTTACCAAAGGGCTAAATTAGTTAATACTCGCAATTATGTTATTCATCACGGTTGACATAGTCCTTGATCTTCATCCTTTAAAAGCATTCTTACCATCATTTTTCTACACATTTTTTTGCCTGCTGCACTAGTGTAAGTTTTGAATAATGCTGAGTATTGAGATGCCCTTGCAGAGAATGAAAGAATTGCAAGAACCCATGGAAGAAAAGATGCTTCGGTTGAGTGTAGAAAATGGGGGATGTTCTGGGTTTCAATATGTTTTCAATTTGGATGACAAAACCAATCCAGATGACAGGTCTATTAAATAGCTTATACTCACTTCTTAATTTGGATTTTCACCTAAAGATTGCCAATGATGTTTGCTTTCACTCTTTAAAACTTCATCCTTTTCCCCCCTTTCATGTTTGCAGGGTGTGTGAGAAGGAAGGAGTTAAATTGGTAGTTGATAACATCTCATATGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAGGAGCTGATTCGTTCCGCTTTCATTGTGAGTAAACCTATGTCTCACCATTTGCTGGATCACCATTGTTGCTTAGATATGTTTATTTTAGTTTCAAAATGTTACACTTTTAGTTAATAAGTTTTCCTTCGATTTTAATTTAGCCTTTAGATTTCAAAATATTATGCTTTTAGACCTTAAAATTTGAATTTTATTTTAATCTAGTAACAAGTTTCAAGATTTACACTTTTTAACCTCAATTTTTAAATAACATATTCACTCCAATCTTTATCTTTAATATCTAAAATTAATTATGAAGTGAAGTTCCAAAATTAACTTCAACATTGATGAAGAAATAATGAAACATAATCCATTATAGTTTTCTTAATTATTTTTACTTAGTCTTTAAAAGTGAGTATTGATTTAAAAGTGAGTATTGAGTGAAAATTGAGATTGAAAATGTAAATCTTGAGTTTAGGGACCAAATTGAAACAAAACTCAAACACTTATATCAAACGGTAACATTTTGAAATCTAAGGATTAAATTGAAATCAAACTCAAAACTTAATCATTAAAGCTTTTGTCCATTTAGTTTCTTTTTTAAAAATCAAGTTTATTAACATTGCTTCCTTTTATTACTTTCCATGCTTTGTTGTCTCAAAATCATAGCCAAAATCTGAAAACTAAAAAGGTAAATTTCAAACGCTTGTTTTTATTTCTAGAAATTGACTAAAGAACTTTATGGGCGTTTGGGACAAAGTGACCACAAATCCAAGTGATGTTGAGGGAGGTTATGGTTCCTAAAGTACTTCTAAATTTTGCTATAACATATAAATAGTTTAGTCTATTTTTTTATACGTAAAATGCTCCAAACAAAAAAAAACTAAATAGTTACTAAACGACATTTAAAAGGGTAATATTTTGAAATCTAAAAGTTGAACCGAAACTAAATCTAAAACCTGGAGACCAAAAAACACATCTCATTTTACATCATATATTTTATGCATTCTTATAAATCCAAAACTTGGAGACCAAAGAACATATATATCTCATTTTACATCATATATTTGATCCATTCTTATTTCTTATTTCACAACAAAAGTAGTGCTAATGCAGAAGTTGAATTCTTGTAATATGCCATATGCAGGTGACCACAAATCCAAGTGCTGTTGGTGGCTGTAGTTGTAAAAGTTCTTTTATGGTGAAACAATAATCATCACAAAGCTCTTCTCAGGAAGGCATAGAAGCAATAACCAAAACTCTGACTTGAAGATATAATGGATTTGTTTATCACTTGGTTGGTTCAGTTGATAAGGCTTTTTGTCCATTCCGATAATTTTTGTATCATGTGCCGCTTTTATATTACTTGAGAACTTCATACTTCATTCTTTGTTGCACTTAATCATGATGGATTTTATTACAAGAGAGTTGCATTGGATAATAAAATATACTTGTATATTTGCAAGTCTTTTAAAA

mRNA sequence

ATGGTTCCCAGATTTCTAATTCAGCGGCTAACACCATATTTGGGAGCACATATCCGAAAGAACCAAAGGCTTCTTAGCTCTTCTGTTTCAGCCTATCAAGAAGCCTCTTCTTCGTTGATAACTCCATCTGCGGGCATTGATACAATTCACATGTCAGATAACTGCATTCGGAGAATGAAAGAATTGCAAGAACCCATGGAAGAAAAGATGCTTCGGTTGAGTGTAGAAAATGGGGGATGTTCTGGGTTTCAATATGTTTTCAATTTGGATGACAAAACCAATCCAGATGACAGGGTGTGTGAGAAGGAAGGAGTTAAATTGGTAGTTGATAACATCTCATATGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAGGAGCTGATTCGTTCCGCTTTCATTGTGACCACAAATCCAAGTGCTGTTGGTGGCTGTAGTTGTAAAAGTTCTTTTATGGTGAAACAATAA

Coding sequence (CDS)

ATGGTTCCCAGATTTCTAATTCAGCGGCTAACACCATATTTGGGAGCACATATCCGAAAGAACCAAAGGCTTCTTAGCTCTTCTGTTTCAGCCTATCAAGAAGCCTCTTCTTCGTTGATAACTCCATCTGCGGGCATTGATACAATTCACATGTCAGATAACTGCATTCGGAGAATGAAAGAATTGCAAGAACCCATGGAAGAAAAGATGCTTCGGTTGAGTGTAGAAAATGGGGGATGTTCTGGGTTTCAATATGTTTTCAATTTGGATGACAAAACCAATCCAGATGACAGGGTGTGTGAGAAGGAAGGAGTTAAATTGGTAGTTGATAACATCTCATATGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAGGAGCTGATTCGTTCCGCTTTCATTGTGACCACAAATCCAAGTGCTGTTGGTGGCTGTAGTTGTAAAAGTTCTTTTATGGTGAAACAATAA

Protein sequence

MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ*

Homology

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BLAST of Csa1G108280.2 vs. Swiss-Prot
Match: ISAM2_ARATH (Iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Arabidopsis thaliana GN=At5g03905 PE=2 SV=2)

HSP 1 Score: 207.2 bits (526), Expect = 1.3e-52
Identity = 110/156 (70.51%), Postives = 125/156 (80.13%), Query Frame = 1


Query: 4   RFLIQRLTPYLGAHIRKNQRLLS-SSVSAYQEASSSLITP--SAGIDTIHMSDNCIRRMK 63
           R L++R+ PYL   IR+N RLL+ SS SA +EASSS  +   S+  D +H+SDNCIRRMK
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASSSSSSQPESSSNDVVHLSDNCIRRMK 62

Query: 64  ELQ--EPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVK 123
           ELQ  EP E+KMLRL VE GGCSGFQY F LD++TNPDDRV EK GVKLVVDN+SYD VK
Sbjct: 63  ELQSSEP-EKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYDLVK 122

Query: 124 GATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVK 155
           GATIDY EELIR+AF+V  NPSAVGGCSCKSSFMVK
Sbjct: 123 GATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMVK 157

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BLAST of Csa1G108280.2 vs. Swiss-Prot
Match: ISCA2_BOVIN (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Bos taurus GN=ISCA2 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 5.2e-30
Identity = 70/135 (51.85%), Postives = 87/135 (64.44%), Query Frame = 1


Query: 20  KNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMKELQEPMEEKMLRLSVENGG 79
           +  RLL++S         S  +P AG   IH++D+C++R+ E+ E  E   LRL VE GG
Sbjct: 19  QRSRLLAASRGPRALREVSFSSPEAGEGQIHLTDSCVQRLLEITEGSE--FLRLEVEGGG 78

Query: 80  CSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFIVTTNP 139
           CSGFQY F+LD   NPDDRV EK G ++VVD+ S  FVKGA +D+ +ELIRS+F V  NP
Sbjct: 79  CSGFQYKFSLDTVINPDDRVFEKGGARVVVDSDSLAFVKGAHVDFSQELIRSSFQVLNNP 138

Query: 140 SAVGGCSCKSSFMVK 155
            A  GCSC SSF VK
Sbjct: 139 QAQQGCSCGSSFSVK 151

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BLAST of Csa1G108280.2 vs. Swiss-Prot
Match: ISCA2_HUMAN (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Homo sapiens GN=ISCA2 PE=1 SV=2)

HSP 1 Score: 131.0 bits (328), Expect = 1.2e-29
Identity = 72/134 (53.73%), Postives = 92/134 (68.66%), Query Frame = 1


Query: 23  RLLSSSVS--AYQEASSSLITPSAGIDTIHMSDNCIRRMKELQEPMEEKMLRLSVENGGC 82
           RLL++S+   A +EASSS  +P AG   I ++D+C++R+ E+ E  E   LRL VE GGC
Sbjct: 24  RLLTASLGPQARREASSS--SPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGGGC 83

Query: 83  SGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFIVTTNPS 142
           SGFQY F+LD   NPDDRV E+ G ++VVD+ S  FVKGA +D+ +ELIRS+F V  NP 
Sbjct: 84  SGFQYKFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQ 143

Query: 143 AVGGCSCKSSFMVK 155
           A  GCSC SSF +K
Sbjct: 144 AQQGCSCGSSFSIK 153

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BLAST of Csa1G108280.2 vs. Swiss-Prot
Match: ISCA2_PONAB (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Pongo abelii GN=ISCA2 PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.2e-29
Identity = 72/134 (53.73%), Postives = 92/134 (68.66%), Query Frame = 1


Query: 23  RLLSSSVS--AYQEASSSLITPSAGIDTIHMSDNCIRRMKELQEPMEEKMLRLSVENGGC 82
           RLL++S+   A +EASSS  +P AG   I ++D+C++R+ E+ E  E   LRL VE GGC
Sbjct: 24  RLLAASLGPQARREASSS--SPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGGGC 83

Query: 83  SGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFIVTTNPS 142
           SGFQY F+LD   NPDDRV E+ G ++VVD+ S  FVKGA +D+ +ELIRS+F V  NP 
Sbjct: 84  SGFQYKFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQ 143

Query: 143 AVGGCSCKSSFMVK 155
           A  GCSC SSF +K
Sbjct: 144 AQQGCSCGSSFSIK 153

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BLAST of Csa1G108280.2 vs. Swiss-Prot
Match: ISCA2_MOUSE (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Mus musculus GN=Isca2 PE=1 SV=2)

HSP 1 Score: 125.6 bits (314), Expect = 4.9e-28
Identity = 67/136 (49.26%), Postives = 86/136 (63.24%), Query Frame = 1


Query: 19  RKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMKELQEPMEEKMLRLSVENG 78
           R++ RLL+          ++   P AG   I ++D+C++R+ E+ E  E   LRL VE G
Sbjct: 20  RRSGRLLAVFPRLLTRWETTSSIPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGG 79

Query: 79  GCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFIVTTN 138
           GCSGFQY F+LD   NPDDRV E+ G ++VVD+ S  FVKGA +D+ +ELIRS+F V  N
Sbjct: 80  GCSGFQYKFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVLNN 139

Query: 139 PSAVGGCSCKSSFMVK 155
           P A  GCSC SSF VK
Sbjct: 140 PQAQQGCSCGSSFSVK 153

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BLAST of Csa1G108280.2 vs. TrEMBL
Match: A0A0A0LSN2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G108280 PE=4 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 6.8e-69
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 1


Query: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60
           MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK
Sbjct: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60

Query: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120
           ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA
Sbjct: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120

Query: 121 TIDYVEELIRSAFI 135
           TIDYVEELIRSAFI
Sbjct: 121 TIDYVEELIRSAFI 134

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BLAST of Csa1G108280.2 vs. TrEMBL
Match: A0A0A0LXX8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G496290 PE=4 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 3.5e-65
Identity = 130/157 (82.80%), Postives = 141/157 (89.81%), Query Frame = 1


Query: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEAS--SSLITPSAGIDTIHMSDNCIRR 60
           M PR LIQR+TPYL A I++NQRLLSSS SAYQEA   S   +PSA +DTIHM+D+CIRR
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRR 60

Query: 61  MKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVK 120
           MKELQ+P EEKMLRLSVE GGCSGFQYVFNLD KTNPDDR+ EKEGVKLVVDNISYDFVK
Sbjct: 61  MKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK 120

Query: 121 GATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 156
           GATIDYVEELIRSAF+V+TNPSAVGGCSCKSSFMVKQ
Sbjct: 121 GATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 157

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BLAST of Csa1G108280.2 vs. TrEMBL
Match: M5WAY8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012679mg PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.6e-54
Identity = 117/159 (73.58%), Postives = 131/159 (82.39%), Query Frame = 1


Query: 1   MVPRFLI-QRLTPYLGAHIRKNQRLLSSSV---SAYQEASSSLITPSAGIDTIHMSDNCI 60
           M PR LI  RLTP+  A IR+NQRLLSSS    SA +EASSS  + S  +D + M+D C+
Sbjct: 1   MAPRSLILHRLTPFFAARIRQNQRLLSSSFYSSSAVEEASSSSSSSSPSLDAVVMTDTCV 60

Query: 61  RRMKELQ-EPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYD 120
           +RMK+LQ    E KMLRLSVE GGCSGFQYVF+LDDKT+PDDRV EKEGVKLVVDN+SYD
Sbjct: 61  QRMKQLQASEAEGKMLRLSVETGGCSGFQYVFDLDDKTHPDDRVFEKEGVKLVVDNVSYD 120

Query: 121 FVKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVK 155
           FVKGAT+DYVEELIRSAF VTTNPSAVGGCSCKSSFMVK
Sbjct: 121 FVKGATVDYVEELIRSAFQVTTNPSAVGGCSCKSSFMVK 159

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BLAST of Csa1G108280.2 vs. TrEMBL
Match: A0A140B491_PRUPE (Fe-S cluster assembly transfer protein 19 OS=Prunus persica var. persica GN=ISA3 PE=2 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.6e-54
Identity = 117/159 (73.58%), Postives = 131/159 (82.39%), Query Frame = 1


Query: 1   MVPRFLI-QRLTPYLGAHIRKNQRLLSSSV---SAYQEASSSLITPSAGIDTIHMSDNCI 60
           M PR LI  RLTP+  A IR+NQRLLSSS    SA +EASSS  + S  +D + M+D C+
Sbjct: 1   MAPRSLILHRLTPFFAARIRQNQRLLSSSFYSSSAVEEASSSSSSSSPSLDAVVMTDTCV 60

Query: 61  RRMKELQ-EPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYD 120
           +RMK+LQ    E KMLRLSVE GGCSGFQYVF+LDDKT+PDDRV EKEGVKLVVDN+SYD
Sbjct: 61  QRMKQLQASEAEGKMLRLSVETGGCSGFQYVFDLDDKTHPDDRVFEKEGVKLVVDNVSYD 120

Query: 121 FVKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVK 155
           FVKGAT+DYVEELIRSAF VTTNPSAVGGCSCKSSFMVK
Sbjct: 121 FVKGATVDYVEELIRSAFQVTTNPSAVGGCSCKSSFMVK 159

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BLAST of Csa1G108280.2 vs. TrEMBL
Match: A0A0D2QDP8_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_002G158400 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.6e-54
Identity = 115/158 (72.78%), Postives = 132/158 (83.54%), Query Frame = 1


Query: 4   RFLIQRLTPYLGAHIRKNQRLLSSSVS----AYQEASSSLITPSAGIDTIHMSDNCIRRM 63
           R LIQR++P+  A I++N +LLSSS S    A  EASS   +PS  +D +HM+DNCI+RM
Sbjct: 11  RSLIQRISPFFIARIKQNHKLLSSSFSSSSSALHEASSE--SPSPSLDAVHMTDNCIKRM 70

Query: 64  KELQ--EPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFV 123
           KELQ  E   ++MLRLSVE GGCSGFQYVF+LDDKTNPDDRV  +EGVKLVVD+ISYDFV
Sbjct: 71  KELQASEQSSDQMLRLSVETGGCSGFQYVFDLDDKTNPDDRVFVREGVKLVVDDISYDFV 130

Query: 124 KGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 156
           KGATIDYVEELIRSAF+VTTNPSAVGGCSCKSSFMVKQ
Sbjct: 131 KGATIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 166

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BLAST of Csa1G108280.2 vs. TAIR10
Match: AT5G03905.1 (AT5G03905.1 Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 207.2 bits (526), Expect = 7.2e-54
Identity = 110/156 (70.51%), Postives = 125/156 (80.13%), Query Frame = 1


Query: 4   RFLIQRLTPYLGAHIRKNQRLLS-SSVSAYQEASSSLITP--SAGIDTIHMSDNCIRRMK 63
           R L++R+ PYL   IR+N RLL+ SS SA +EASSS  +   S+  D +H+SDNCIRRMK
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASSSSSSQPESSSNDVVHLSDNCIRRMK 62

Query: 64  ELQ--EPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVK 123
           ELQ  EP E+KMLRL VE GGCSGFQY F LD++TNPDDRV EK GVKLVVDN+SYD VK
Sbjct: 63  ELQSSEP-EKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYDLVK 122

Query: 124 GATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVK 155
           GATIDY EELIR+AF+V  NPSAVGGCSCKSSFMVK
Sbjct: 123 GATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMVK 157

[Close]

BLAST of Csa1G108280.2 vs. TAIR10
Match: AT1G10500.1 (AT1G10500.1 chloroplast-localized ISCA-like protein)

HSP 1 Score: 70.1 bits (170), Expect = 1.4e-12
Identity = 43/142 (30.28%), Postives = 70/142 (49.30%), Query Frame = 1


Query: 13  YLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMKELQ-EPMEEKML 72
           Y+  ++ +  RL   S S     +   + P+     I +S+N ++ + +++ E  E+  L
Sbjct: 41  YVNLNLNRRNRLSVRSASVPAAPAMEGLKPA-----ISLSENALKHLSKMRSERGEDLCL 100

Query: 73  RLSVENGGCSGFQYVFNLDDKTN--PDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELI 132
           R+ V+ GGCSG  Y  + +++ N  PDD   E +G  +V D  S  F+ G  +DY + LI
Sbjct: 101 RIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYSDALI 160

Query: 133 RSAFIVTTNPSAVGGCSCKSSF 152
              F   +NP+A   C C  SF
Sbjct: 161 GGGFSF-SNPNATQTCGCGKSF 176

[Close]

BLAST of Csa1G108280.2 vs. TAIR10
Match: AT2G36260.1 (AT2G36260.1 Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 69.3 bits (168), Expect = 2.3e-12
Identity = 38/101 (37.62%), Postives = 57/101 (56.44%), Query Frame = 1


Query: 51  MSDNCIRRMKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVD 110
           +SD    R+++L +  ++  LRL+VE  GC+G  YV N   +    D V E++GVK++VD
Sbjct: 8   LSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKGVKILVD 67

Query: 111 NISYDFVKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSF 152
             +   V G  +D+V++ +RS F V  NP+A   C C  SF
Sbjct: 68  PKAVMHVIGTEMDFVDDKLRSEF-VFVNPNAT-KCGCGESF 106

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BLAST of Csa1G108280.2 vs. NCBI nr
Match: gi|449459108|ref|XP_004147288.1| (PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X1 [Cucumis sativus])

HSP 1 Score: 310.8 bits (795), Expect = 1.3e-81
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 1


Query: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60
           MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK
Sbjct: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60

Query: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120
           ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA
Sbjct: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120

Query: 121 TIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 156
           TIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 TIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 155

[Close]

BLAST of Csa1G108280.2 vs. NCBI nr
Match: gi|778659089|ref|XP_011653808.1| (PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X2 [Cucumis sativus])

HSP 1 Score: 282.3 bits (721), Expect = 5.0e-73
Identity = 142/144 (98.61%), Postives = 142/144 (98.61%), Query Frame = 1


Query: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60
           MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK
Sbjct: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60

Query: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120
           ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA
Sbjct: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120

Query: 121 TIDYVEELIRSAFIVTTNPSAVGG 145
           TIDYVEELIRSAFIVTTNPS V G
Sbjct: 121 TIDYVEELIRSAFIVTTNPSDVEG 144

[Close]

BLAST of Csa1G108280.2 vs. NCBI nr
Match: gi|700209707|gb|KGN64803.1| (hypothetical protein Csa_1G108280 [Cucumis sativus])

HSP 1 Score: 268.1 bits (684), Expect = 9.8e-69
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 1


Query: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60
           MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK
Sbjct: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60

Query: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120
           ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA
Sbjct: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120

Query: 121 TIDYVEELIRSAFI 135
           TIDYVEELIRSAFI
Sbjct: 121 TIDYVEELIRSAFI 134

[Close]

BLAST of Csa1G108280.2 vs. NCBI nr
Match: gi|449470384|ref|XP_004152897.1| (PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucumis sativus])

HSP 1 Score: 255.8 bits (652), Expect = 5.0e-65
Identity = 130/157 (82.80%), Postives = 141/157 (89.81%), Query Frame = 1


Query: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEAS--SSLITPSAGIDTIHMSDNCIRR 60
           M PR LIQR+TPYL A I++NQRLLSSS SAYQEA   S   +PSA +DTIHM+D+CIRR
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRR 60

Query: 61  MKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVK 120
           MKELQ+P EEKMLRLSVE GGCSGFQYVFNLD KTNPDDR+ EKEGVKLVVDNISYDFVK
Sbjct: 61  MKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK 120

Query: 121 GATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 156
           GATIDYVEELIRSAF+V+TNPSAVGGCSCKSSFMVKQ
Sbjct: 121 GATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 157

[Close]

BLAST of Csa1G108280.2 vs. NCBI nr
Match: gi|659115367|ref|XP_008457521.1| (PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 253.8 bits (647), Expect = 1.9e-64
Identity = 129/159 (81.13%), Postives = 141/159 (88.68%), Query Frame = 1


Query: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPS----AGIDTIHMSDNCI 60
           M PR LIQR+TPYL A I++NQRLLSSS +AYQEASS   +PS    A +DTIHM+D+CI
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSATAYQEASSLSSSPSPSPSAPVDTIHMTDSCI 60

Query: 61  RRMKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDF 120
           RRMKELQ+P EEKMLRLSVE GGCSGFQYVFNLD KT PDDR+ EKEGVKLVVDNISYDF
Sbjct: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTTPDDRIYEKEGVKLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 156
           VKGATIDYVEELIRSAF+V+TNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 159

The following BLAST results are available for this feature:

BLAST of Csa1G108280.2 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ISAM2_ARATH	1.3e-52	70.51	Iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Arabidopsis thaliana ...	[more]
ISCA2_BOVIN	5.2e-30	51.85	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Bos taurus GN=ISCA2 PE=...	[more]
ISCA2_HUMAN	1.2e-29	53.73	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Homo sapiens GN=ISCA2 P...	[more]
ISCA2_PONAB	1.2e-29	53.73	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Pongo abelii GN=ISCA2 P...	[more]
ISCA2_MOUSE	4.9e-28	49.26	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Mus musculus GN=Isca2 P...	[more]

BLAST of Csa1G108280.2 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LSN2_CUCSA	6.8e-69	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G108280 PE=4 SV=1	[more]
A0A0A0LXX8_CUCSA	3.5e-65	82.80	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G496290 PE=4 SV=1	[more]
M5WAY8_PRUPE	1.6e-54	73.58	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012679mg PE=4 SV=1	[more]
A0A140B491_PRUPE	1.6e-54	73.58	Fe-S cluster assembly transfer protein 19 OS=Prunus persica var. persica GN=ISA3...	[more]
A0A0D2QDP8_GOSRA	1.6e-54	72.78	Uncharacterized protein OS=Gossypium raimondii GN=B456_002G158400 PE=4 SV=1	[more]

BLAST of Csa1G108280.2 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G03905.1	7.2e-54	70.51	Iron-sulphur cluster biosynthesis family protein	[more]
AT1G10500.1	1.4e-12	30.28	chloroplast-localized ISCA-like protein	[more]
AT2G36260.1	2.3e-12	37.62	Iron-sulphur cluster biosynthesis family protein	[more]

BLAST of Csa1G108280.2 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449459108\|ref\|XP_004147288.1\|	1.3e-81	100.00	PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X1 [C...	[more]
gi\|778659089\|ref\|XP_011653808.1\|	5.0e-73	98.61	PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X2 [C...	[more]
gi\|700209707\|gb\|KGN64803.1\|	9.8e-69	100.00	hypothetical protein Csa_1G108280 [Cucumis sativus]	[more]
gi\|449470384\|ref\|XP_004152897.1\|	5.0e-65	82.80	PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucumis sati...	[more]
gi\|659115367\|ref\|XP_008457521.1\|	1.9e-64	81.13	PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X2 [C...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR000361	FeS_biogenesis
IPR016092	FeS_cluster_insertion

Vocabulary: **Molecular Function**
Term	Definition
GO:0005198	structural molecule activity
GO:0051536	iron-sulfur cluster binding

Vocabulary: **Biological Process**
Term	Definition
GO:0016226	iron-sulfur cluster assembly

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0005198	structural molecule activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Csa1G108280	Csa1G108280	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Csa1G108280.2	Csa1G108280.2-protein	polypeptide

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csa1G108280.2.utr3p1	Csa1G108280.2.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csa1G108280.2.cds4	Csa1G108280.2.cds4	CDS
Csa1G108280.2.cds3	Csa1G108280.2.cds3	CDS
Csa1G108280.2.cds2	Csa1G108280.2.cds2	CDS
Csa1G108280.2.cds1	Csa1G108280.2.cds1	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000361	FeS cluster biogenesis	GENE3D	G3DSA:2.60.300.12		coord: 48..139 score: 5.5
IPR000361	FeS cluster biogenesis	PFAM	PF01521	Fe-S_biosyn	coord: 49..148 score: 3.9
IPR000361	FeS cluster biogenesis	unknown	SSF89360	HesB-like domain	coord: 48..141 score: 1.96
IPR016092	FeS cluster insertion protein	TIGRFAMs	TIGR00049	TIGR00049	coord: 49..153 score: 2.0
None	No IPR available	PANTHER	PTHR10072	IRON-SULFUR CLUSTER ASSEMBLY PROTEIN	coord: 3..154 score: 3.5
None	No IPR available	PANTHER	PTHR10072:SF46	IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL	coord: 3..154 score: 3.5