Cp4.1LG02g12680 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG02g12680
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Shugoshin-1, putative
Location	Cp4.1LG02 : 11635308 .. 11639205 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGATTCAAAAGGGTAACGTTCAAATATTTAAAATCTTGGTTCGGAGAGGTAGGTTCGGAATTGATTTAATTTAGCCCTCTCGGCTTTACCGATTCGGAAAGCCGTTGTCCGGCCGAAGCTCTCAACTATTGCAGAATCTTCGTCTTCAGCTTTTTTGCTTTGTTCCTTTGAATCTTGAATCTCTGCAATTAGGGCTTTTCTTTCCTCTCTAATTTCCTCTGTTTTCTCTTCCATAAAAACCGCAATTTCCTCTCATCATAGACACCACAAATCCCCAATTATTGGCGAAATTCCTTCCTTAGGGCATTGTGTTACGATTCGCTGATTTGAAATGGCGAAGCGATTGTCGATCGGAAGCAGAGTGCGGAACAAGCTAGCAGATATTACGAATTCCAAGACAATGAGAGCTCATATGGAAGATGAAAGGTCTTCGGAAGCTTGGCGGTCAAATCAAGACGTTGTCGATCAGCTGGTCGAGGTATGCATCAATCTGGTCTCCCTTTGCCTGTTTTTCTTTAATCTGAGATATTCGTTTGATTGGATGTAATTTATGGATCAGGAAAATATGGCTCTGATGAAACTTGTCATGGAGAAGAAGTATCCTTCTGGATTTGCCTTTTCTTCCTCTAAATTCTCTATTATCAATCTCGTTAGCATATCATGTCAATACTATCGTAGTTCATATCTTTTGAAAACAGAACTTTCAAGGAAAACAAATGATTCCTTCACATTTGAACAGTGAAATTATTGATCTGACTGAAGCCGAGCTAAACAAACTTCGTGAATGCATTCAGAAACTGCAGCTTCAGAATTGGCAGCTTGCTCAATCAAACAGTCATTTTTTAGCAGTATGATCATTTATAATTCAGAAACATTTATGATTATCCCTCTCCTATTTTTCATTTCCCTTGACAACTCTGAGACTCTAATTAACTGTTCATCGATTTCCTCTTACAGGAAATCAATTTAGGGAGAGAGAGAGTAAGTTCCCAAGAATCTTGAATTTATCTTTTGCTGCATTTGTTTGAATCTCATTTATGCTCAATAAATTGGTCCTGATGTGTTTCAGATAAAATCACTGGGGCATGAGCTTGAATGCAAGGAGGCCTTGCTAAAAGCAACGCGTTTGAATACTGTGGTACTAACTTTAGACACTTTTTCTTCCTGCAAAGAAAANAAATCACTGGGGCATGAGCTTGAATGCAAGGAGGCCTTGCTAAAAGCAACGCGTTTGAATACTGTGGTACTAACTTTACACACTTTTTCTTCCTGCAAAGAATAGTAGGGATATCTATTTTGTCTAGCTCTGGAATTGTTTCAGTCATTTATGAATATGTTTTCAATAAGTAGTCAGATCATCATTTAGATAATCATTTAGATAATGACAGAGATAATGTTAGAATTGGAACCAAAATTGTAATTTAACCAACAATATGAAATTAGGAGTAATCTTGATCAAGTTATTTTGTGAAGTGATTCGAATCACGAGTTTAGAAATGAGTTTTCTTTACTTTTCATTCTAAAACTTTTCCTTGGATTGATTCCTTGTCGTTTTTTGAGGTTTTTGGATAATTTAGAGTTAAAGGTCTTGCTCTTTAACAAGGTTTTTAGATCAAATCGTAGGAATTTCATAAAACGTATTTATTTGTTATTAACATTTCGACACTCAGCATNGAATATGTTTTCAATAAGTAGTCAGATCATCATTTAGATAATGACAGAGATAATGTTAGAATTGGAACTAAAATTGTAATTTAACCAACAATATGAAATTAATATGAAATTAGGAGTACTCTTGATCAAGTTATTTTGTGAAGTGATTCGAATCACGAGTTTAGAAATGCTTTTGATTCCAAAACTTTTCCTTGGATTGATTCCTTATCGTTTTTTGAGGTTCTTGGATAATTTAGATTTAAAGGTCTTGCTCTTTAACAAGGTTTTTAGATCAAATCGTAGGAATTTCATAAAACGTATTTATTTGTTATTAACATTTCGACACTCAACAATCACAACAGATCCTTACAAACTAGACCCAACTTGCTTTTGGGGAAAACTGAAGCTTATCTTATCAAGATTTAGATTAGCTAGATTAATTTTCTTCAATAGGCAATGAAAAAGTCTTGTATTTTAACAAGAAACTGCTGTATATTCCGACAGGGGAAAGCAGGAATGAATAATGGAAATTCTGGATGGCAGGTAATTGGTAATTTCCTTTTTTTTTTTCTTTCAGGCATCTGCTTTTCTATTCTTTTCATGCCTCAAATTTCACTTAGAACTGTCTTTTTCACCCATTTTTTCCTGGCTTCAGGAAGAAGAGAAGCCAACAGAGCAATTTCCACTTGCGGTTAAAACTGACACCAAAGCTTGCAGTGGAAACAGAAAGCCCTCCGGACGAGCTAGAAATCAATGTATGTTCATGAATTGATGAATATGAACAAAAGCCTAAAAATGTTCCCATTTTAATGCCAATTTATAGCAACTATCCTCACCGTNAGCCCTCCGGACGAGCTAGAAATCAATGTATGTTCATGAATTAATGAACATGAACAAAAGCCTAAAAATGTTCCATTTTACTGCCAATTTATAGCAACTATCCTCACCGTTATGTTAAAATTGTGAGTTGCAGTAAAAAGGGTTTCATAAGAAATTATTCTGATGCTAAGTTCTACTCTTTTGAAAATGCTTTTAATAAAGTTATTCTCTAGGACTGTTTCCTCATAAATCACTTCTAAATCTTTTTCAAGAACATGTTACTCCCAAATGTTAGGATGGTTTTGAGACCATGCCAAGAGGACCTACGTTTGGAATTACGGTTCTCCACGATGGTATGATATTGTCCACTTTGAGTGAGTATAAACTCTCATGGCTTTGCTTTAGGCTTCCCAGCCTAATACCAATGGAGATGAATTCCTTACTTATAAACCCATGATCATNACGGTTCTCCACGATGGTATGATATTGTCCACTTTGAGTGAGCATAAACTCTCATGGCTTTGCTTTAGGCTTCCCCGCCCGATACCAATGGAGATGTATTCCTTACCTATAAACTCATGATCATTTCCTAAATTAGCAGCTAACGTGGGACTCCCTCCACACAATCCTCAACGTCCTAAACCTCCTAAACCTGAAAACTTTATTCTTATTGTCTGGTTGAAAAGTAACGACACTTAAACAACTGCAGCTATGAGCCCTTCAGCTTCATACTCAAAGTGTGCGATGAAGAGAAAGGATGAAAACAAAAGGTACTTACCAATATTTAAACTGGTATTCTTTCAACTTTAGTTACTGCTTTCTTTACATATTTCTGTTTACAGGCATTGTGTAAGAGCACAATCTGGTAGATTTAGATATCAAATGAGACACCATGAAGAGAACTCGTTTGAGATAGAAGAAGACATGAAATTTACTGCTACTGGAGAAGATGAAGAAGAAAGGAAGAACAACAATGTCAATTCACCATCATCATCTTCAGCTCTGCAAAGATCTTATATTGGAAGACCATTGCACAGAGCAGCTGAAAAGATTCAATCCTACAAAGAAGCTCGTCACAACGCTAAAATCAGACGACAAGAATGACAAGTGAGATCAGTATACTGTTTCAGCTAGCAGTTTAAATTTTACTTTCTTGTATGTAGTAGCTATGATTATTCCACCTGTGGCTTGTATTGTTCGTGATGTTTATATATTATTTTGTTTCGAGTGTATGTATAGTGTAAATTATGTAACTGTCAAAGAGAAGAGATTCGAACTTCTAACTTCTTAAAAAGGAGACGATGTTGTAATTGAAATTTGACATTTTGGAGTAAGAAATTCGAACCTCTATTCTTTTAATAATCTTGATTTGGATAGTATAGTCGATGAATTTGACTCATGATCTGTAATGATAGCCATTTAACAA

mRNA sequence

GGATTCAAAAGGGTAACGTTCAAATATTTAAAATCTTGGTTCGGAGAGGTAGGTTCGGAATTGATTTAATTTAGCCCTCTCGGCTTTACCGATTCGGAAAGCCGTTGTCCGGCCGAAGCTCTCAACTATTGCAGAATCTTCGTCTTCAGCTTTTTTGCTTTGTTCCTTTGAATCTTGAATCTCTGCAATTAGGGCTTTTCTTTCCTCTCTAATTTCCTCTGTTTTCTCTTCCATAAAAACCGCAATTTCCTCTCATCATAGACACCACAAATCCCCAATTATTGGCGAAATTCCTTCCTTAGGGCATTGTGTTACGATTCGCTGATTTGAAATGGCGAAGCGATTGTCGATCGGAAGCAGAGTGCGGAACAAGCTAGCAGATATTACGAATTCCAAGACAATGAGAGCTCATATGGAAGATGAAAGGTCTTCGGAAGCTTGGCGGTCAAATCAAGACGTTGTCGATCAGCTGGTCGAGGAAAATATGGCTCTGATGAAACTTGTCATGGAGAAGAATGAAATTATTGATCTGACTGAAGCCGAGCTAAACAAACTTCGTGAATGCATTCAGAAACTGCAGCTTCAGAATTGGCAGCTTGCTCAATCAAACAGTCATTTTTTAGCAGAAATCAATTTAGGGAGAGAGAGAATAAAATCACTGGGGCATGAGCTTGAATGCAAGGAGGCCTTGCTAAAAGCAACGCGTTTGAATACTGTGGGGAAAGCAGGAATGAATAATGGAAATTCTGGATGGCAGGAAGAAGAGAAGCCAACAGAGCAATTTCCACTTGCGGTTAAAACTGACACCAAAGCTTGCAGTGGAAACAGAAAGCCCTCCGGACGAGCTAGAAATCAATCTATGAGCCCTTCAGCTTCATACTCAAAGTGTGCGATGAAGAGAAAGGATGAAAACAAAAGGCATTGTGTAAGAGCACAATCTGGTAGATTTAGATATCAAATGAGACACCATGAAGAGAACTCGTTTGAGATAGAAGAAGACATGAAATTTACTGCTACTGGAGAAGATGAAGAAGAAAGGAAGAACAACAATGTCAATTCACCATCATCATCTTCAGCTCTGCAAAGATCTTATATTGGAAGACCATTGCACAGAGCAGCTGAAAAGATTCAATCCTACAAAGAAGCTCGTCACAACGCTAAAATCAGACGACAAGAATGACAAGTGAGATCAGTATACTGTTTCAGCTAGCAGTTTAAATTTTACTTTCTTGTATGTAGTAGCTATGATTATTCCACCTGTGGCTTGTATTGTTCGTGATGTTTATATATTATTTTGTTTCGAGTGTATGTATAGTGTAAATTATGTAACTGTCAAAGAGAAGAGATTCGAACTTCTAACTTCTTAAAAAGGAGACGATGTTGTAATTGAAATTTGACATTTTGGAGTAAGAAATTCGAACCTCTATTCTTTTAATAATCTTGATTTGGATAGTATAGTCGATGAATTTGACTCATGATCTGTAATGATAGCCATTTAACAA

Coding sequence (CDS)

ATGGCGAAGCGATTGTCGATCGGAAGCAGAGTGCGGAACAAGCTAGCAGATATTACGAATTCCAAGACAATGAGAGCTCATATGGAAGATGAAAGGTCTTCGGAAGCTTGGCGGTCAAATCAAGACGTTGTCGATCAGCTGGTCGAGGAAAATATGGCTCTGATGAAACTTGTCATGGAGAAGAATGAAATTATTGATCTGACTGAAGCCGAGCTAAACAAACTTCGTGAATGCATTCAGAAACTGCAGCTTCAGAATTGGCAGCTTGCTCAATCAAACAGTCATTTTTTAGCAGAAATCAATTTAGGGAGAGAGAGAATAAAATCACTGGGGCATGAGCTTGAATGCAAGGAGGCCTTGCTAAAAGCAACGCGTTTGAATACTGTGGGGAAAGCAGGAATGAATAATGGAAATTCTGGATGGCAGGAAGAAGAGAAGCCAACAGAGCAATTTCCACTTGCGGTTAAAACTGACACCAAAGCTTGCAGTGGAAACAGAAAGCCCTCCGGACGAGCTAGAAATCAATCTATGAGCCCTTCAGCTTCATACTCAAAGTGTGCGATGAAGAGAAAGGATGAAAACAAAAGGCATTGTGTAAGAGCACAATCTGGTAGATTTAGATATCAAATGAGACACCATGAAGAGAACTCGTTTGAGATAGAAGAAGACATGAAATTTACTGCTACTGGAGAAGATGAAGAAGAAAGGAAGAACAACAATGTCAATTCACCATCATCATCTTCAGCTCTGCAAAGATCTTATATTGGAAGACCATTGCACAGAGCAGCTGAAAAGATTCAATCCTACAAAGAAGCTCGTCACAACGCTAAAATCAGACGACAAGAATGA

Protein sequence

MAKRLSIGSRVRNKLADITNSKTMRAHMEDERSSEAWRSNQDVVDQLVEENMALMKLVMEKNEIIDLTEAELNKLRECIQKLQLQNWQLAQSNSHFLAEINLGRERIKSLGHELECKEALLKATRLNTVGKAGMNNGNSGWQEEEKPTEQFPLAVKTDTKACSGNRKPSGRARNQSMSPSASYSKCAMKRKDENKRHCVRAQSGRFRYQMRHHEENSFEIEEDMKFTATGEDEEERKNNNVNSPSSSSALQRSYIGRPLHRAAEKIQSYKEARHNAKIRRQE

Homology

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BLAST of Cp4.1LG02g12680 vs. Swiss-Prot
Match: SGO1_MAIZE (Shugoshin-1 OS=Zea mays GN=SGO1 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.5e-11
Identity = 68/198 (34.34%), Postives = 96/198 (48.48%), Query Frame = 1


Query: 15  LADITNSKTMRAHMEDERSSEAWRSNQDVVDQLVEENMALMKLVMEKNEIIDLTEAELNK 74
           LADITN+    A            +    V  LV+EN  L+ L+ EK +IIDL+  E+ K
Sbjct: 42  LADITNTGKPNA------------ARSITVPDLVKENTKLLTLLNEKTKIIDLSRVEIYK 101

Query: 75  LRECIQKLQLQNWQLAQSNSHFLAEINLGRERIKSLGHELECKEALLKATRLNTVGKAGM 134
           LR  +Q  + QN  L Q+NS  LAEIN G++RIK L HEL C  ALLK      V  + +
Sbjct: 102 LRLALQASKQQNLHLTQTNSQMLAEINTGKDRIKMLQHELSCTTALLK------VKDSEL 161

Query: 135 NNGNSGWQEEEKPTEQFPLAVKTDTKACS----GNRKPSGRARN--QSMSPSASYSKCAM 194
           +   +    ++K  +   L  K  T A      G+   SG   +  +S S  +S + C  
Sbjct: 162 DRKKNAGNVQQKGVKSQVLKTKASTVAVEAHHVGDSVTSGVEHHVVESQSAVSSNTVCQE 221

Query: 195 KRKD-ENKRHCVRAQSGR 206
             +D + KR   R +S R
Sbjct: 222 PPQDGKQKRMPQRRRSSR 221

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BLAST of Cp4.1LG02g12680 vs. TrEMBL
Match: A0A0A0LSM0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G097670 PE=4 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 3.9e-83
Identity = 188/285 (65.96%), Postives = 220/285 (77.19%), Query Frame = 1


Query: 1   MAKRLSIGSRVRNKLADITNSKTMRAHMEDERSSEAWRSNQDVVDQLVEENMALMKLVME 60
           MAK  SIGS++RNKLADITNSKTMR+H+EDE SSEA +  + V+DQLV+ENM LMKL+ME
Sbjct: 1   MAKTSSIGSKMRNKLADITNSKTMRSHLEDENSSEASQPKKSVIDQLVKENMGLMKLIME 60

Query: 61  KNEIIDLTEAELNKLRECIQKLQLQNWQLAQSNSHFLAEINLGRERIKSLGHELECKEAL 120
           +N+II+L+EAEL KLR  IQKLQLQNW LAQSNS FLAEINLGR+RIKSL HE+ECKEAL
Sbjct: 61  RNKIIELSEAELEKLRASIQKLQLQNWHLAQSNSRFLAEINLGRKRIKSLEHEIECKEAL 120

Query: 121 LKATRLNTVGKAGMNNGNSGWQEEEKPTEQFPLA-VKTDTKACSGNRKPSGRARNQSMSP 180
           L+A RL   GKA MNN NS WQE EK T Q  LA V TDTK+ +GN KP GR R++SMSP
Sbjct: 121 LRAKRLTVEGKAEMNNRNSEWQEGEKATGQPSLAIVNTDTKSGNGNIKPPGRTRSKSMSP 180

Query: 181 SASYSKCAMKRKDENKRHCVRAQSGRFRYQMRHHEENSFEIEEDMKFTATGEDEEERKNN 240
           S SYS    K+K  NKRHCVR  S RFR+Q+R  EE  FEIE D+K  A      E++ N
Sbjct: 181 STSYSTVVDKQKVVNKRHCVRRHSSRFRHQVRDLEEKLFEIE-DIKLIAG-----EKEKN 240

Query: 241 NVNSPSSS--SALQRSYIGRPLHRAAEKIQSYKEARHNAKIRRQE 283
           + +SP S   SALQR+ IGRPL RAAEKI+SYKE+R N K+RRQE
Sbjct: 241 SPSSPRSEEVSALQRTSIGRPLRRAAEKIRSYKESRLNIKLRRQE 279

[Close]

BLAST of Cp4.1LG02g12680 vs. TrEMBL
Match: A0A061GYW9_THECC (Shugoshin C terminus, putative OS=Theobroma cacao GN=TCM_042157 PE=4 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.6e-47
Identity = 132/300 (44.00%), Postives = 181/300 (60.33%), Query Frame = 1


Query: 1   MAKRLSIGSRVRNKLADITNSKTM-RAHMEDERSSEAWRSNQDVVDQLVEENMALMKLVM 60
           MAKR S GS +R +L+DITNS+T  +   ++E+  +   + +D ++QL++E M LMKL+ 
Sbjct: 6   MAKRSSFGSMMRKRLSDITNSQTQPKPSCQEEKPQQISAATEDYINQLIKEKMTLMKLIE 65

Query: 61  EKNEIIDLTEAELNKLRECIQKLQLQNWQLAQSNSHFLAEINLGRERIKSLGHELECKEA 120
           E+N+II+L+  EL  LR C+QKLQLQNW LAQSNS  LAE+NLGR+++K+L HEL CK+A
Sbjct: 66  ERNKIIELSGTELQNLRNCLQKLQLQNWNLAQSNSQMLAELNLGRDKVKALQHELVCKDA 125

Query: 121 LLKATRLNTVGKAGMNNGNSGWQEE---EKPTEQFPLAVKTDTKACSGNRKPSGRARNQS 180
           LLKA  L   GKA +N  N+G   E   +   E        D K  + NR+ S   R+QS
Sbjct: 126 LLKAKNLEKKGKADINCQNTGLLGETGAQAAVECIQPKANDDDKPSNRNRRRS--TRSQS 185

Query: 181 MSPSASYSKCAMKRKDENKRHCVRAQSGRFRYQMRHHEENSFEIEEDMKFTAT------- 240
           M PS +  + A K K E+KR C+R QS RF+ Q R   +N FEI ED+ + A        
Sbjct: 186 MGPSTTSQRGADKEKIESKRRCLRRQSARFKSQEREPTKNLFEI-EDVNYAAAQQLDTPM 245

Query: 241 GEDEEE-------RKNNNVNSPSSSSALQRSYIGRPLHRAAEKIQSYKEARHNAKIRRQE 283
            ED+          K    N  +    L+R   GRPL +AAEK+QSYKE   N K+RR++
Sbjct: 246 HEDDPTPSLVSSITKEEACNPMTGKQILKRPSFGRPLRKAAEKVQSYKEVPLNVKMRRED 302

[Close]

BLAST of Cp4.1LG02g12680 vs. TrEMBL
Match: V4SFX3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10028947mg PE=4 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.0e-46
Identity = 135/297 (45.45%), Postives = 182/297 (61.28%), Query Frame = 1


Query: 1   MAKRLSIGSRVRNKLADITNS----KTMRAHMEDERSSEAWRSNQDVVDQLVEENMALMK 60
           MAK  S GS +R +L+DITNS    K + +H  +E+ S+   ++ D+VDQL++E   LM+
Sbjct: 1   MAKGSSFGSIMRRRLSDITNSQSRPKVLLSH--EEKPSQVSPASGDLVDQLIKEKTTLMR 60

Query: 61  LVMEKNEIIDLTEAELNKLRECIQKLQLQNWQLAQSNSHFLAEINLGRERIKSLGHELEC 120
            + E+N+II+L+ +EL+ LR  IQKLQLQNW LAQSNS +LAEINLGRE++K+L HEL C
Sbjct: 61  FIEERNKIIELSSSELHNLRISIQKLQLQNWNLAQSNSQYLAEINLGREKVKALQHELVC 120

Query: 121 KEALLKATRLNTVGKAGMNNGNSGWQEEEKPTEQFPLAVKTDTKACSGNRKPSGRARNQS 180
           K+AL+KA  +    K   N  N+  QE EK  E+       + K C  NR+ S R +  S
Sbjct: 121 KDALIKAKSIVKERKTYSNCENTASQEGEKVIEECVPKANENVKTCERNRRRSTRCK--S 180

Query: 181 MSPSASYSKCAMKRKDENKRHCVRAQSGRFRYQMRHHEENSFEIEEDMKFTATGE-DEEE 240
           M PS +  K A K   ENKR CVR QS RF+ Q R   EN FEI ED K  AT   D+  
Sbjct: 181 MGPSTTRQKVAEKENVENKRRCVRRQSARFKSQERAPTENLFEI-EDSKLPATQPLDDPM 240

Query: 241 RKNNNVNSPSSSS------------ALQRSYIGRPLHRAAEKIQSYKEARHNAKIRR 281
            ++N++ + SS++              QRS +GRP  +AAEK+QSYKE     K+RR
Sbjct: 241 HEDNSIQAGSSTANEEFSSSRNEARLSQRSSMGRPSRKAAEKVQSYKELPLKVKMRR 292

[Close]

BLAST of Cp4.1LG02g12680 vs. TrEMBL
Match: B9SCJ5_RICCO (Shugoshin-1, putative OS=Ricinus communis GN=RCOM_0474400 PE=4 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.0e-43
Identity = 135/299 (45.15%), Postives = 183/299 (61.20%), Query Frame = 1


Query: 1   MAKRLSIGSRVRNKLADITNSKTMRAHME-DERSSEAWRSNQDVVDQLVEENMALMKLVM 60
           +AKR S GS VR +L+DITNS++    +  +E+        + +++QL++E + LMKLV 
Sbjct: 6   VAKRSSFGSIVRKRLSDITNSQSQTKLVNIEEKQPPISDPTEGLINQLLKEKVTLMKLVE 65

Query: 61  EKNEIIDLTEAELNKLRECIQKLQLQNWQLAQSNSHFLAEINLGRERIKSLGHELECKEA 120
           EK++II L+  ++  LR   QKLQ+QNW LAQSNS  LAEINLGRE++KSL HEL CK+A
Sbjct: 66  EKDKIIALSNNQMRSLRLHYQKLQMQNWNLAQSNSQMLAEINLGREKLKSLQHELICKDA 125

Query: 121 LLKATRLNTVGKAGMNNGNSGWQEEEKPTEQFPLAVKTDTKACSGNRKPSGR-ARNQSMS 180
           LLKA  L   GK  + + N+G QE EK      L  K    +  GN +   R AR+QSM 
Sbjct: 126 LLKAKNLELEGKTDITSQNNGSQEAEKNAVGECL-YKASNGSKPGNHRIRRRVARSQSMG 185

Query: 181 PSASYSK-CAMKRKDENKRHCVRAQSGRFRYQMRHHEENSFEIEEDMKFTAT-------G 240
           PS + S+  A K K ENKR C+R QS RF+ Q R   EN FEI ED+  + T        
Sbjct: 186 PSTTSSRQDAEKEKLENKRRCLRRQSARFKSQEREPSENLFEI-EDVTLSITQQLDNPVQ 245

Query: 241 EDEEERKNNNV-------NSPSSSSALQRSYIGRPLHRAAEKIQSYKEARHNAKIRRQE 283
           ED +    ++V       +S + +   QRS +GRP+ RAAEK+QSYKEA  N K+RR++
Sbjct: 246 EDAQTLPESSVIKEEETCDSRTEAQVPQRSSLGRPVRRAAEKVQSYKEAPINVKLRRKD 302

[Close]

BLAST of Cp4.1LG02g12680 vs. TrEMBL
Match: U5GGX7_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s22560g PE=4 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.7e-41
Identity = 134/300 (44.67%), Postives = 174/300 (58.00%), Query Frame = 1


Query: 1   MAKRLSIGSRVRNKLADITNSKTMRAHM----EDERSSEAWRSNQDVVDQLV---EENMA 60
           M KR S G+ +R +L+DITN+++    +    E  R  E     QD++DQL+   +E   
Sbjct: 6   MEKRSSFGNIMRRRLSDITNTQSQSKFVGLIEEQPRIPEC---TQDLIDQLLLVKQEKAV 65

Query: 61  LMKLVMEKNEIIDLTEAELNKLRECIQKLQLQNWQLAQSNSHFLAEINLGRERIKSLGHE 120
           L+KLV E+N+II+L+  +L  LR   Q LQLQNW LAQSNS  LAE+NLGRE++K+L HE
Sbjct: 66  LLKLVEERNKIIELSGNKLRDLRMNYQNLQLQNWNLAQSNSQMLAELNLGREKLKALQHE 125

Query: 121 LECKEALLKATRLNTVGKAGMNNGNSGWQEEEKPTEQFPLA-VKTDTKACSGNRKPSGRA 180
           + CK+AL KA  L   GKA +N  N+  QE EK  E   L     D K C  + + +GR+
Sbjct: 126 VVCKDALHKARNLEAQGKADVNCQNAVSQEVEKIEEAECLPEASNDIKPCGRSGRRTGRS 185

Query: 181 RNQSMSPSASYSKCAMKRKDENKRHCVRAQSGRFRYQMRHHEENSFEIEEDMKFTATGED 240
           R  SM PS +  K A K K E KR CVR QS RF+ Q R   E  FEIE   KF  + + 
Sbjct: 186 R--SMGPSTTNRKTAEKEKTETKRRCVRRQSARFKSQEREPAEKLFEIEV-AKFPVSRD- 245

Query: 241 EEERKNNNVNSP----------SSSSALQRSYIGRPLHRAAEKIQSYKEARHNAKIRRQE 283
            + RK N + S           + +    RS IGRPL RAAEK+QSYKE   N K+RR E
Sbjct: 246 -KSRKENGLTSSITKEETCGAGNEAQVSLRSSIGRPLRRAAEKVQSYKEVPVNVKMRRAE 297

[Close]

BLAST of Cp4.1LG02g12680 vs. TAIR10
Match: AT5G04320.2 (AT5G04320.2 Shugoshin C terminus)

HSP 1 Score: 77.0 bits (188), Expect = 2.0e-14
Identity = 41/97 (42.27%), Postives = 64/97 (65.98%), Query Frame = 1


Query: 29  EDERSSEAWRSNQDVVDQLVEENMALMKLVMEKNEIIDLTEAELNKLRECIQKLQLQNWQ 88
           E ++      S+Q+   +L +ENM LMK +  +N++++L+  E+ KLR  ++ +Q +N Q
Sbjct: 29  ETQQKENMLFSSQEYAAKLQKENMTLMKALAHRNKLVELSGIEIQKLRINLRSVQEKNLQ 88

Query: 89  LAQSNSHFLAEINLGRERIKSLGHELECKEALLKATR 126
           LAQ+NS  LAE+N  R+R+K L HEL CK ALLK  +
Sbjct: 89  LAQANSQMLAELNTNRDRLKDLQHELGCKNALLKVKK 125

[Close]

BLAST of Cp4.1LG02g12680 vs. TAIR10
Match: AT3G10440.1 (AT3G10440.1 Shugoshin C terminus)

HSP 1 Score: 66.6 bits (161), Expect = 2.8e-11
Identity = 64/202 (31.68%), Postives = 95/202 (47.03%), Query Frame = 1


Query: 7   IGSRVRNKLADITNSKTMRAHMEDERSSEAWR---SNQDVVDQLVEENMALMKLVMEKNE 66
           + S  R KL DITN +  +  M      +      S+++  + L +EN  LMK+VME++ 
Sbjct: 33  MNSAQRRKLGDITNLQNQKNLMNQGAKHQQQAILISSKENAENLQKENTKLMKVVMERDG 92

Query: 67  IIDLTEAELNKLRECIQKLQLQNWQLAQSNSHFLAEINLGRERIKSLGHELECKEALLKA 126
           I    +++L KLR   QK+Q QN  LAQ+N+  LAE N  ++++K L HEL CK  L+ A
Sbjct: 93  I----KSDLKKLRIEFQKVQEQNLLLAQANTRILAEFNTSKDQLKVLQHELGCKNGLVMA 152

Query: 127 TRLNTVGKAGMNNGNSGWQEEEKPTEQFPLAVKTDTKACSGNRKPSGRARNQSMSPSASY 186
            ++               +E+  P  +    VK    AC G  K      N +    AS 
Sbjct: 153 RKMLL-------------KEQTLPCTRHASKVKAQANACGGACKTF--QPNDADHEHASG 212

Query: 187 SKCAMKRKDENKRHCVRAQSGR 206
           S  A   +   K +  R  SGR
Sbjct: 213 SSNANSLQRNEKANSKRRVSGR 215

[Close]

BLAST of Cp4.1LG02g12680 vs. NCBI nr
Match: gi|449459158|ref|XP_004147313.1| (PREDICTED: uncharacterized protein LOC101205365 [Cucumis sativus])

HSP 1 Score: 316.2 bits (809), Expect = 5.7e-83
Identity = 188/285 (65.96%), Postives = 220/285 (77.19%), Query Frame = 1


Query: 1   MAKRLSIGSRVRNKLADITNSKTMRAHMEDERSSEAWRSNQDVVDQLVEENMALMKLVME 60
           MAK  SIGS++RNKLADITNSKTMR+H+EDE SSEA +  + V+DQLV+ENM LMKL+ME
Sbjct: 1   MAKTSSIGSKMRNKLADITNSKTMRSHLEDENSSEASQPKKSVIDQLVKENMGLMKLIME 60

Query: 61  KNEIIDLTEAELNKLRECIQKLQLQNWQLAQSNSHFLAEINLGRERIKSLGHELECKEAL 120
           +N+II+L+EAEL KLR  IQKLQLQNW LAQSNS FLAEINLGR+RIKSL HE+ECKEAL
Sbjct: 61  RNKIIELSEAELEKLRASIQKLQLQNWHLAQSNSRFLAEINLGRKRIKSLEHEIECKEAL 120

Query: 121 LKATRLNTVGKAGMNNGNSGWQEEEKPTEQFPLA-VKTDTKACSGNRKPSGRARNQSMSP 180
           L+A RL   GKA MNN NS WQE EK T Q  LA V TDTK+ +GN KP GR R++SMSP
Sbjct: 121 LRAKRLTVEGKAEMNNRNSEWQEGEKATGQPSLAIVNTDTKSGNGNIKPPGRTRSKSMSP 180

Query: 181 SASYSKCAMKRKDENKRHCVRAQSGRFRYQMRHHEENSFEIEEDMKFTATGEDEEERKNN 240
           S SYS    K+K  NKRHCVR  S RFR+Q+R  EE  FEIE D+K  A      E++ N
Sbjct: 181 STSYSTVVDKQKVVNKRHCVRRHSSRFRHQVRDLEEKLFEIE-DIKLIAG-----EKEKN 240

Query: 241 NVNSPSSS--SALQRSYIGRPLHRAAEKIQSYKEARHNAKIRRQE 283
           + +SP S   SALQR+ IGRPL RAAEKI+SYKE+R N K+RRQE
Sbjct: 241 SPSSPRSEEVSALQRTSIGRPLRRAAEKIRSYKESRLNIKLRRQE 279

[Close]

BLAST of Cp4.1LG02g12680 vs. NCBI nr
Match: gi|659072061|ref|XP_008463238.1| (PREDICTED: uncharacterized protein LOC103501433 isoform X2 [Cucumis melo])

HSP 1 Score: 310.1 bits (793), Expect = 4.1e-81
Identity = 183/285 (64.21%), Postives = 220/285 (77.19%), Query Frame = 1


Query: 1   MAKRLSIGSRVRNKLADITNSKTMRAHMEDERSSEAWRSNQDVVDQLVEENMALMKLVME 60
           MAK  SIGS++RNKLADITNSKTMRAH+EDE S +A ++ + V+DQLVEENM LMKL+ME
Sbjct: 1   MAKTSSIGSKMRNKLADITNSKTMRAHLEDENSFKASQTRKSVIDQLVEENMGLMKLIME 60

Query: 61  KNEIIDLTEAELNKLRECIQKLQLQNWQLAQSNSHFLAEINLGRERIKSLGHELECKEAL 120
           +N+II+L+EAEL KLR  IQKLQLQNW LAQSNS FLAEINLGR+RIKSL HE+ECKEAL
Sbjct: 61  RNKIIELSEAELEKLRASIQKLQLQNWHLAQSNSRFLAEINLGRKRIKSLEHEIECKEAL 120

Query: 121 LKATRLNTVGKAGMNNGNSGWQEEEKPTEQFPLA-VKTDTKACSGNRKPSGRARNQSMSP 180
           L+A  L   GK+ MNN NS WQE E+ T +  LA V TDTK+C+GN KP GR R+QSMSP
Sbjct: 121 LRAKCLTVEGKSEMNNRNSEWQEGERATGKPSLAIVNTDTKSCNGNIKPPGRTRSQSMSP 180

Query: 181 SASYSKCAMKRKDENKRHCVRAQSGRFRYQMRHHEENSFEIEEDMKFTATGEDEEERKNN 240
           S SYS    K+K  NKRH VR  S RFR+Q+R  EE  FEIE D+K  A      E++ N
Sbjct: 181 STSYSTVVDKQKVVNKRHFVRRHSSRFRHQVRDLEEKLFEIE-DIKLVAG-----EKEKN 240

Query: 241 NVNSP--SSSSALQRSYIGRPLHRAAEKIQSYKEARHNAKIRRQE 283
           +++SP    +SALQR+ IGRPL RAAEKI+SYKE+R N K+RR+E
Sbjct: 241 SLSSPRIEEASALQRTSIGRPLRRAAEKIRSYKESRLNIKLRRRE 279

[Close]

BLAST of Cp4.1LG02g12680 vs. NCBI nr
Match: gi|659072059|ref|XP_008463230.1| (PREDICTED: uncharacterized protein LOC103501433 isoform X1 [Cucumis melo])

HSP 1 Score: 307.8 bits (787), Expect = 2.0e-80
Identity = 184/286 (64.34%), Postives = 221/286 (77.27%), Query Frame = 1


Query: 1   MAKRLSIGSRVRNKLADITNSKTMRAHMEDERSSEAWRSNQDVVDQLVEENMALMKLVME 60
           MAK  SIGS++RNKLADITNSKTMRAH+EDE S +A ++ + V+DQLVEENM LMKL+ME
Sbjct: 1   MAKTSSIGSKMRNKLADITNSKTMRAHLEDENSFKASQTRKSVIDQLVEENMGLMKLIME 60

Query: 61  KNEIIDLTEAELNKLRECIQKLQLQNWQLAQSNSHFLAEINLGRERIKSLGHELECKEAL 120
           +N+II+L+EAEL KLR  IQKLQLQNW LAQSNS FLAEINLGR+RIKSL HE+ECKEAL
Sbjct: 61  RNKIIELSEAELEKLRASIQKLQLQNWHLAQSNSRFLAEINLGRKRIKSLEHEIECKEAL 120

Query: 121 LKATRLNT-VGKAGMNNGNSGWQEEEKPTEQFPLA-VKTDTKACSGNRKPSGRARNQSMS 180
           L+A  L   VGK+ MNN NS WQE E+ T +  LA V TDTK+C+GN KP GR R+QSMS
Sbjct: 121 LRAKCLTVEVGKSEMNNRNSEWQEGERATGKPSLAIVNTDTKSCNGNIKPPGRTRSQSMS 180

Query: 181 PSASYSKCAMKRKDENKRHCVRAQSGRFRYQMRHHEENSFEIEEDMKFTATGEDEEERKN 240
           PS SYS    K+K  NKRH VR  S RFR+Q+R  EE  FEIE D+K  A      E++ 
Sbjct: 181 PSTSYSTVVDKQKVVNKRHFVRRHSSRFRHQVRDLEEKLFEIE-DIKLVAG-----EKEK 240

Query: 241 NNVNSP--SSSSALQRSYIGRPLHRAAEKIQSYKEARHNAKIRRQE 283
           N+++SP    +SALQR+ IGRPL RAAEKI+SYKE+R N K+RR+E
Sbjct: 241 NSLSSPRIEEASALQRTSIGRPLRRAAEKIRSYKESRLNIKLRRRE 280

[Close]

BLAST of Cp4.1LG02g12680 vs. NCBI nr
Match: gi|659072063|ref|XP_008463245.1| (PREDICTED: uncharacterized protein LOC103501433 isoform X3 [Cucumis melo])

HSP 1 Score: 283.5 bits (724), Expect = 4.1e-73
Identity = 174/285 (61.05%), Postives = 211/285 (74.04%), Query Frame = 1


Query: 1   MAKRLSIGSRVRNKLADITNSKTMRAHMEDERSSEAWRSNQDVVDQLVEENMALMKLVME 60
           MAK  SIGS++RNKLADITNSKTMRAH+EDE S +A ++ + V+DQLVEENM LMKL+ME
Sbjct: 1   MAKTSSIGSKMRNKLADITNSKTMRAHLEDENSFKASQTRKSVIDQLVEENMGLMKLIME 60

Query: 61  KNEIIDLTEAELNKLRECIQKLQLQNWQLAQSNSHFLAEINLGRERIKSLGHELECKEAL 120
           +N+II+L+EAEL KLR  IQKLQLQNW LAQSNS FLAEINLGR+RIKSL HE+ECKEAL
Sbjct: 61  RNKIIELSEAELEKLRASIQKLQLQNWHLAQSNSRFLAEINLGRKRIKSLEHEIECKEAL 120

Query: 121 LKATRLNTVGKAGMNNGNSGWQEEEKPTEQFPLA-VKTDTKACSGNRKPSGRARNQSMSP 180
           L+A  L               +E E+ T +  LA V TDTK+C+GN KP GR R+QSMSP
Sbjct: 121 LRAKCLTV-------------EEGERATGKPSLAIVNTDTKSCNGNIKPPGRTRSQSMSP 180

Query: 181 SASYSKCAMKRKDENKRHCVRAQSGRFRYQMRHHEENSFEIEEDMKFTATGEDEEERKNN 240
           S SYS    K+K  NKRH VR  S RFR+Q+R  EE  FEIE D+K  A      E++ N
Sbjct: 181 STSYSTVVDKQKVVNKRHFVRRHSSRFRHQVRDLEEKLFEIE-DIKLVAG-----EKEKN 240

Query: 241 NVNSP--SSSSALQRSYIGRPLHRAAEKIQSYKEARHNAKIRRQE 283
           +++SP    +SALQR+ IGRPL RAAEKI+SYKE+R N K+RR+E
Sbjct: 241 SLSSPRIEEASALQRTSIGRPLRRAAEKIRSYKESRLNIKLRRRE 266

[Close]

BLAST of Cp4.1LG02g12680 vs. NCBI nr
Match: gi|1009115938|ref|XP_015874505.1| (PREDICTED: shugoshin-1-like [Ziziphus jujuba])

HSP 1 Score: 201.8 bits (512), Expect = 1.6e-48
Identity = 139/302 (46.03%), Postives = 187/302 (61.92%), Query Frame = 1


Query: 1   MAKRLSIGSRVRNKLADITNSK-TMRAHMEDERSSEAWRSNQDVVDQLVEENMALMKLVM 60
           MAKR S GS +R KL+DITNS+ T     +DE+  E ++ ++D VDQL+ E + LMKL+ 
Sbjct: 6   MAKRASFGSIMRKKLSDITNSQATKTTTHQDEKPQEIYQPDKDYVDQLIRERITLMKLLA 65

Query: 61  EKNEIIDLTEAELNKLRECIQKLQLQNWQLAQSNSHFLAEINLGRERIKSLGHELECKEA 120
           E+N+II+L+  EL K+R   QKLQLQNW LAQSNS  LAE++LGRE++K+L HEL CK+A
Sbjct: 66  ERNKIIELSGEELQKMRASFQKLQLQNWNLAQSNSQMLAELHLGREKVKALQHELLCKDA 125

Query: 121 LLKATRLNTVGKAGMNNGNSGWQEEEKPTEQFPLAVKTDTKACSGNRKPSGRARNQSMSP 180
           LL+A +L   GKA +N  N   ++E +      +A  TD K CS NR+ + R+R  SM  
Sbjct: 126 LLRAKKLELEGKAEVNCHNKIARQEGQQAAL--VAASTDDKPCSQNRRRTTRSR--SMGS 185

Query: 181 SASYSKCAMKRKDENKRHCVRAQSGRFRYQMRHHEENSFEIEEDMKFTATG--------- 240
           S++  K   K K ENKR C+R QS RF+   R    N FEI+ D+K +AT          
Sbjct: 186 SSTCQKGEDKDKVENKRRCLRRQSARFKSHERE-PGNLFEID-DVKISATEALSNPTSNE 245

Query: 241 ----------EDEEERKNNNVNSPSSSSALQRSYIGRPLHRAAEKIQSYKEARHNAKIRR 283
                     ++E+E +    +S S S   QRS IGRPL RA EK+QSYKE   N K+RR
Sbjct: 246 WSTPLHASAVKEEKEEEIGRASSRSESGVSQRSSIGRPLRRAVEKVQSYKEVPLNVKMRR 301

The following BLAST results are available for this feature:

BLAST of Cp4.1LG02g12680 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SGO1_MAIZE	1.5e-11	34.34	Shugoshin-1 OS=Zea mays GN=SGO1 PE=2 SV=1	[more]

BLAST of Cp4.1LG02g12680 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LSM0_CUCSA	3.9e-83	65.96	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G097670 PE=4 SV=1	[more]
A0A061GYW9_THECC	1.6e-47	44.00	Shugoshin C terminus, putative OS=Theobroma cacao GN=TCM_042157 PE=4 SV=1	[more]
V4SFX3_9ROSI	1.0e-46	45.45	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10028947mg PE=4 SV=1	[more]
B9SCJ5_RICCO	4.0e-43	45.15	Shugoshin-1, putative OS=Ricinus communis GN=RCOM_0474400 PE=4 SV=1	[more]
U5GGX7_POPTR	1.7e-41	44.67	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s22560g PE=4 SV=1	[more]

BLAST of Cp4.1LG02g12680 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G04320.2	2.0e-14	42.27	Shugoshin C terminus	[more]
AT3G10440.1	2.8e-11	31.68	Shugoshin C terminus	[more]

BLAST of Cp4.1LG02g12680 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449459158\|ref\|XP_004147313.1\|	5.7e-83	65.96	PREDICTED: uncharacterized protein LOC101205365 [Cucumis sativus]	[more]
gi\|659072061\|ref\|XP_008463238.1\|	4.1e-81	64.21	PREDICTED: uncharacterized protein LOC103501433 isoform X2 [Cucumis melo]	[more]
gi\|659072059\|ref\|XP_008463230.1\|	2.0e-80	64.34	PREDICTED: uncharacterized protein LOC103501433 isoform X1 [Cucumis melo]	[more]
gi\|659072063\|ref\|XP_008463245.1\|	4.1e-73	61.05	PREDICTED: uncharacterized protein LOC103501433 isoform X3 [Cucumis melo]	[more]
gi\|1009115938\|ref\|XP_015874505.1\|	1.6e-48	46.03	PREDICTED: shugoshin-1-like [Ziziphus jujuba]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0043229	intracellular organelle
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG02g12680.1	Cp4.1LG02g12680.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	unknown	Coil	Coil	coord: 259..279 score: -coord: 65..85 scor
None	No IPR available	PANTHER	PTHR34373	FAMILY NOT NAMED	coord: 1..282 score: 1.2
None	No IPR available	PANTHER	PTHR34373:SF3	SUBFAMILY NOT NAMED	coord: 1..282 score: 1.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG02g12680	Cucsa.207680	Cucumber (Gy14) v1	cgycpeB0583
Cp4.1LG02g12680	CmaCh01G005210	Cucurbita maxima (Rimu)	cmacpeB491
Cp4.1LG02g12680	CmoCh01G005530	Cucurbita moschata (Rifu)	cmocpeB449
Cp4.1LG02g12680	Cla005078	Watermelon (97103) v1	cpewmB548
Cp4.1LG02g12680	Csa1G097670	Cucumber (Chinese Long) v2	cpecuB528
Cp4.1LG02g12680	MELO3C004971	Melon (DHL92) v3.5.1	cpemeB490
Cp4.1LG02g12680	ClCG03G002610	Watermelon (Charleston Gray)	cpewcgB500
Cp4.1LG02g12680	CSPI01G13170	Wild cucumber (PI 183967)	cpecpiB530
Cp4.1LG02g12680	MELO3C004971.2	Melon (DHL92) v3.6.1	cpemedB583
Cp4.1LG02g12680	CsaV3_1G013340	Cucumber (Chinese Long) v3	cpecucB0657
Cp4.1LG02g12680	Bhi08G000229	Wax gourd	cpewgoB0707
Cp4.1LG02g12680	CsGy1G013280	Cucumber (Gy14) v2	cgybcpeB090
Cp4.1LG02g12680	Carg12298	Silver-seed gourd	carcpeB0622

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG02g12680	Silver-seed gourd	carcpeB1489
Cp4.1LG02g12680	Wax gourd	cpewgoB0695
Cp4.1LG02g12680	Cucurbita pepo (Zucchini)	cpecpeB432
Cp4.1LG02g12680	Cucurbita maxima (Rimu)	cmacpeB238
Cp4.1LG02g12680	Cucurbita moschata (Rifu)	cmocpeB205
Cp4.1LG02g12680	Cucumber (Gy14) v2	cgybcpeB386