Cp4.1LG02g10060 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG02g10060
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPectate lyase
LocationCp4.1LG02 : 9910324 .. 9912284 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACTCAGCTCATTATCCTGTCGCTCATTTCCGTGGCACTCCTGGCCGCCGTTTCCCACACGGAAGCCCGCGCCATGAACATGAATGCCATCGACCGATGCTGGCGACCGAACCCATACTGGAGAAAAAACAGGCAAAAGCTCGCCCTTTGTTCAATTGGGTTTGTAGGAAAAATGACCAACAACATAGGAAGAAACCTCGTACACTACCAAGTCACGGACCCCAGCGACGACCCACTAACCCCACGCCCAGGCACCCTCAGATACGGCGCCACAATGATCAAACCCAAAGTGTGGATCACGTTTCAAAAAGACATGCACATTGTGCTTAACAAGCCGCTTCTTATTAGCAGCTACACGGCCATCGACGGCCGTGGCGCCACCGTCCACATCACGGGGAATGCGTGTCTGATGGTGTCTAGAGCCACCAATGTCATAATCCATGGGCTTATTATCCACCATTGCAAGGCCCAGGCGGCTGGCCGTGTAATGGGGCCGGGTTCGAAGATCGTTTCGTTGGGACACGTGGACGGTGATGCCATTCGTTTGGTTAGTGCTTCTAAGATTTGGATTGACCATAACACGCTGTATCGGTGTGAGGATGGGCTGATTGATGTCACTAGAGGCTCTACGGATATCACCATTTCTAACAATTGGTTGAGAGATCAAGATAAGGTTATACTTTTGGGTCACGACGATGATTATTTTCGGGACCGAAACATGAAGGTTACTCTCGTTTATAACCATTTCGGACCTAATTGCAACCAACGGATGCCCAGGTAACATTTAACACGGTATCAAAACATAACATAAATTTTAAGGTTAAATTAATAAAAATATTTGTAAATTTTGTATTTTATCTTAAAAATACTTTTTAAAATTTTCAAATTTTTTAATAATGTTCTTAAACTCAAAAATACCATTAAACTTTAGAATAAATTAAAAAAAATATTTTTATTGTTAATTTTGGATGAAAACTATTAATAATTTGTTTTAAAACACGTTAAAAAAAAGTTTCAAAACTATCTTTCTTAAGTTTTATTGATACCGAGCTTTTTTTTAAAAAAAATATTAATTTTTTTAAAAAGTTAAAAAATAATTCGTATATGAACGAAATTGTTAGTACCCTTTAAAAAAAATAACTAAAAATTTTAATTTTTTTTGTACGGTACCCTTAAAATTGAAGATAGAGATGACATATGAAGTATAGATAGATATTATCCATTACGATAAAACTTTTTAATTTTATTTTACAAAAGTTCACGAATAATTATTAAACATAGTATTAATGGTAAGAGTATTTTTCAGCTTTTTTTACTTGAAAAGCACAACCAAACTTTTGAAAGTTCAAAGATATTTTGANTATTTTTTTTTTTTTTTTTAGTTTAAAGGTAATATTGAAATTCTTATAAATTTATAATTTTGAGACAAAATATAAAGTTTACGGATATTTTCTTTTTTATTAATTTAACAAAATTTGGTATGAAAATACAGGATTCGATACGGGTATGCACACGTGGCGAACAATTTGTACCAAGGTTGGACACAATACGCAATTGGAGGCAGCATGAATCCTAGTGTGAAGAGTGAAGCCAACCTCTTCATAGCATCAAAATCAAAACAGGCAATAATAATAACAACAACAATATATCAACACTCCATATTTGGTTAAAAAATTGAGCCTTTTCTTACCAAACAACAACAAATGCTTATTTGAAAATCATAAAATGCAGATCATATGGAGCACAGGCAAAGTAGAGAACGCCAAGTGGAAGTTCCATTCAGTAAGAGACGTTTTTGAAAACGGCGCTTCCTTCGCACAGATCGGCGCTGGCCGAGGCGGTGTCAAGCCAAATTACAATGCCCTGCAGCGCTTCCCAGTGGTAGACGCCAAGTGGTTGCGGTCATTGACAAGCTCTTCCGGCGCCCTGCGCTGCTCACCACGCTCAAGGTGTTGA

mRNA sequence

ATGGCCACTCAGCTCATTATCCTGTCGCTCATTTCCGTGGCACTCCTGGCCGCCGTTTCCCACACGGAAGCCCGCGCCATGAACATGAATGCCATCGACCGATGCTGGCGACCGAACCCATACTGGAGAAAAAACAGGCAAAAGCTCGCCCTTTGTTCAATTGGGTTTGTAGGAAAAATGACCAACAACATAGGAAGAAACCTCGTACACTACCAAGTCACGGACCCCAGCGACGACCCACTAACCCCACGCCCAGGCACCCTCAGATACGGCGCCACAATGATCAAACCCAAAGTGTGGATCACGTTTCAAAAAGACATGCACATTGTGCTTAACAAGCCGCTTCTTATTAGCAGCTACACGGCCATCGACGGCCGTGGCGCCACCGTCCACATCACGGGGAATGCGTGTCTGATGGTGTCTAGAGCCACCAATGTCATAATCCATGGGCTTATTATCCACCATTGCAAGGCCCAGGCGGCTGGCCGTGTAATGGGGCCGGGTTCGAAGATCGTTTCGTTGGGACACGTGGACGGTGATGCCATTCGTTTGGTTAGTGCTTCTAAGATTTGGATTGACCATAACACGCTGTATCGGTGTGAGGATGGGCTGATTGATGTCACTAGAGGCTCTACGGATATCACCATTTCTAACAATTGGTTGAGAGATCAAGATAAGGTTATACTTTTGGGTCACGACGATGATTATTTTCGGGACCGAAACATGAAGATCATATGGAGCACAGGCAAAGTAGAGAACGCCAAGTGGAAGTTCCATTCAGTAAGAGACGTTTTTGAAAACGGCGCTTCCTTCGCACAGATCGGCGCTGGCCGAGGCGGTGTCAAGCCAAATTACAATGCCCTGCAGCGCTTCCCAGTGGTAGACGCCAAGTGGTTGCGGTCATTGACAAGCTCTTCCGGCGCCCTGCGCTGCTCACCACGCTCAAGGTGTTGA

Coding sequence (CDS)

ATGGCCACTCAGCTCATTATCCTGTCGCTCATTTCCGTGGCACTCCTGGCCGCCGTTTCCCACACGGAAGCCCGCGCCATGAACATGAATGCCATCGACCGATGCTGGCGACCGAACCCATACTGGAGAAAAAACAGGCAAAAGCTCGCCCTTTGTTCAATTGGGTTTGTAGGAAAAATGACCAACAACATAGGAAGAAACCTCGTACACTACCAAGTCACGGACCCCAGCGACGACCCACTAACCCCACGCCCAGGCACCCTCAGATACGGCGCCACAATGATCAAACCCAAAGTGTGGATCACGTTTCAAAAAGACATGCACATTGTGCTTAACAAGCCGCTTCTTATTAGCAGCTACACGGCCATCGACGGCCGTGGCGCCACCGTCCACATCACGGGGAATGCGTGTCTGATGGTGTCTAGAGCCACCAATGTCATAATCCATGGGCTTATTATCCACCATTGCAAGGCCCAGGCGGCTGGCCGTGTAATGGGGCCGGGTTCGAAGATCGTTTCGTTGGGACACGTGGACGGTGATGCCATTCGTTTGGTTAGTGCTTCTAAGATTTGGATTGACCATAACACGCTGTATCGGTGTGAGGATGGGCTGATTGATGTCACTAGAGGCTCTACGGATATCACCATTTCTAACAATTGGTTGAGAGATCAAGATAAGGTTATACTTTTGGGTCACGACGATGATTATTTTCGGGACCGAAACATGAAGATCATATGGAGCACAGGCAAAGTAGAGAACGCCAAGTGGAAGTTCCATTCAGTAAGAGACGTTTTTGAAAACGGCGCTTCCTTCGCACAGATCGGCGCTGGCCGAGGCGGTGTCAAGCCAAATTACAATGCCCTGCAGCGCTTCCCAGTGGTAGACGCCAAGTGGTTGCGGTCATTGACAAGCTCTTCCGGCGCCCTGCGCTGCTCACCACGCTCAAGGTGTTGA

Protein sequence

MATQLIILSLISVALLAAVSHTEARAMNMNAIDRCWRPNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPSDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYFRDRNMKIIWSTGKVENAKWKFHSVRDVFENGASFAQIGAGRGGVKPNYNALQRFPVVDAKWLRSLTSSSGALRCSPRSRC
BLAST of Cp4.1LG02g10060 vs. Swiss-Prot
Match: PLY11_ARATH (Putative pectate lyase 11 OS=Arabidopsis thaliana GN=At3g27400 PE=3 SV=2)

HSP 1 Score: 218.4 bits (555), Expect = 1.1e-55
Identity = 122/286 (42.66%), Postives = 163/286 (56.99%), Query Frame = 1

Query: 30  NAIDRCWRPNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPS-DDPLTPRPGTL 89
           N ID CWR +P W  NRQ+LA C+IGF GK     GR    Y VTDP+ DDP+ PRPGTL
Sbjct: 64  NPIDDCWRCDPNWETNRQRLADCAIGF-GKNAIG-GRKGRIYVVTDPANDDPVNPRPGTL 123

Query: 90  RYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNVII 149
           RY  T  +P +WI F++DM I L K L+I+S+  IDGRG++VHIT   CL +  ATN+II
Sbjct: 124 RYAVTQEEP-LWIIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIII 183

Query: 150 HGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLIDVT 209
           HG+ IH CK  + G +             DGDA+ +     +WIDH +L  C+DGLID  
Sbjct: 184 HGINIHDCKPGSGGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCSLSNCDDGLIDAI 243

Query: 210 RGSTDITISNNWLRDQDKVILLGHDDDYFRDRNMKII-----WSTGKVENAKWKFHSVRD 269
            GST ITISNN +   DKV+LLGH D Y +D+NM++      +  G V+      H    
Sbjct: 244 HGSTAITISNNHMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFH 303

Query: 270 VFENGASFAQIGAGRGGVKPN-YNALQRFPVVDAKWLRSLTSSSGA 309
           V  N  +  ++ A  G   P  Y+   RF   + ++ + +T    A
Sbjct: 304 VVNNDYTHWEMYAIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDA 346

BLAST of Cp4.1LG02g10060 vs. Swiss-Prot
Match: PLY14_ARATH (Putative pectate lyase 14 OS=Arabidopsis thaliana GN=At4g13210 PE=2 SV=2)

HSP 1 Score: 215.3 bits (547), Expect = 9.6e-55
Identity = 119/286 (41.61%), Postives = 160/286 (55.94%), Query Frame = 1

Query: 30  NAIDRCWRPNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPSD-DPLTPRPGTL 89
           N ID CWR +  W + R++LA C+IGF        GR+  +Y VTDPSD DP+TP+PGTL
Sbjct: 90  NPIDDCWRCDKKWHRRRKRLADCAIGFGRNAVG--GRDGRYYIVTDPSDHDPVTPKPGTL 149

Query: 90  RYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNVII 149
           RY     +P +WI F++DM I L++ L+++S+  IDGRG  VHI G ACL V   TN+II
Sbjct: 150 RYAVIQDEP-LWIVFKRDMVITLSQELIMNSFKTIDGRGVNVHIAGGACLTVQYVTNIII 209

Query: 150 HGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLIDVT 209
           HG+ IH CK      V    S        DGD I +  +S IWIDHN+L  C DGLID  
Sbjct: 210 HGINIHDCKRTGNAMVRSSESHYGWRTMADGDGISIFGSSHIWIDHNSLSSCADGLIDAI 269

Query: 210 RGSTDITISNNWLRDQDKVILLGHDDDYFRDRNMKII-----WSTGKVENAKWKFHSVRD 269
            GST ITISNN+L   ++ ILLGH D Y RD+ M++      +  G ++      H    
Sbjct: 270 MGSTAITISNNYLTHHNEAILLGHTDSYTRDKMMQVTIAYNHFGEGLIQRMPRCRHGYFH 329

Query: 270 VFENGASFAQIGAGRGGVKPNYNAL-QRFPVVDAKWLRSLTSSSGA 309
           V  N  +  ++ A  G   P  N+   RF     ++ + +T   GA
Sbjct: 330 VVNNDYTHWEMYAIGGSANPTINSQGNRFLAPGNRFAKEVTKRVGA 372

BLAST of Cp4.1LG02g10060 vs. Swiss-Prot
Match: PLY4_ARATH (Probable pectate lyase 4 OS=Arabidopsis thaliana GN=At1g30350 PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 2.8e-54
Identity = 115/250 (46.00%), Postives = 152/250 (60.80%), Query Frame = 1

Query: 1   MATQLIILSLISVALLAAVSHTE------ARAMNMNAIDRCWRPNPYWRKNRQKLALCSI 60
           MA+ ++I+SL+  A  + +  T       A  +++N ID CWR NP W  NRQ LA C++
Sbjct: 1   MASLVVIVSLLLAAFASPLLETAHSYNVTAPRVSLNPIDACWRRNPKWATNRQALAHCAV 60

Query: 61  GFVGKMTNNIGRNLVHYQVTDPSDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKP 120
           G+ GK     G+N   Y VT+PSD+P  P PGTLRY  +  KP +WITF +DM IVL   
Sbjct: 61  GY-GKAAIG-GKNGPIYVVTNPSDNPTRPSPGTLRYAVSQPKP-LWITFARDMVIVLKSQ 120

Query: 121 LLISSYTAIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSL 180
           L+I+SY  IDGRGA V I    CL + +  +VIIHG+ IH CKA   G            
Sbjct: 121 LMINSYKTIDGRGAKVEIANGPCLRIRQVKHVIIHGISIHDCKADPNG------------ 180

Query: 181 GHVDGDAIRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDD 240
             +DGD IR+  ++ +WIDH  L RC DGLIDV   ST +TISNN+    DKV+LLGHDD
Sbjct: 181 --MDGDGIRVFQSTHVWIDHCFLSRCHDGLIDVIVSSTAVTISNNYFTQHDKVMLLGHDD 233

Query: 241 DYFRDRNMKI 245
            Y  D++M++
Sbjct: 241 SYMGDKDMRV 233

BLAST of Cp4.1LG02g10060 vs. Swiss-Prot
Match: PLY9_ARATH (Probable pectate lyase 9 OS=Arabidopsis thaliana GN=At3g24230 PE=2 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 8.1e-54
Identity = 114/266 (42.86%), Postives = 152/266 (57.14%), Query Frame = 1

Query: 28  NMNAIDRCWRPNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPSDDPLT-PRPG 87
           N N ID CWR +  W KNR+ LA C +GF  K     GRN  +Y VTD SDD +  P+PG
Sbjct: 102 NGNLIDDCWRCDRNWNKNRKHLADCGMGFGSKAFG--GRNGSYYVVTDHSDDDVVNPKPG 161

Query: 88  TLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNV 147
           TLR+    ++P +WI F++DM I L + L+++S+  ID RGA VHI   AC+ +   TNV
Sbjct: 162 TLRHAVIQVEP-LWIIFKRDMVIKLKQELIMNSFKTIDARGANVHIANGACITIQNITNV 221

Query: 148 IIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLID 207
           I+HGL IH CK      V    S+    G  DGDAI +  +S IWIDHN+L  C DGL+D
Sbjct: 222 IVHGLHIHDCKRTGNVTVRSSPSQAGFRGTADGDAINIFGSSHIWIDHNSLSNCTDGLVD 281

Query: 208 VTRGSTDITISNNWLRDQDKVILLGHDDDYFRDRNMKII-----WSTGKVENAKWKFHSV 267
           V  GST ITISNN     D+V+LLGH+D Y RD+ M++      +  G ++      H  
Sbjct: 282 VVNGSTAITISNNHFTHHDEVMLLGHNDSYTRDKMMQVTVAYNHFGEGLIQRMPRCRHGY 341

Query: 268 RDVFENGASFAQIGAGRGGVKPNYNA 288
             V  N  +  ++ A  G   P  N+
Sbjct: 342 FHVVNNDYTHWKMYAIGGSANPTINS 364

BLAST of Cp4.1LG02g10060 vs. Swiss-Prot
Match: PLY_ZINVI (Pectate lyase OS=Zinnia violacea PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.1e-53
Identity = 118/286 (41.26%), Postives = 160/286 (55.94%), Query Frame = 1

Query: 30  NAIDRCWRPNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDP-SDDPLTPRPGTL 89
           N ID CWR +P W  NRQ+LA C+IGF GK     GRN   Y VTDP +DDP+ P PGTL
Sbjct: 53  NPIDDCWRCDPNWANNRQRLADCAIGF-GKNAMG-GRNGRIYVVTDPGNDDPVNPVPGTL 112

Query: 90  RYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNVII 149
           RY     +P +WI F++DM I L + L+++S+  IDGRG  VHI    C+ +  A+N+II
Sbjct: 113 RYAVIQDEP-LWIIFKRDMVIQLRQELVMNSHKTIDGRGVNVHIGNGPCITIHYASNIII 172

Query: 150 HGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLIDVT 209
           HG+ IH CK    G +             DGD I + ++  IWIDHN+L  C DGLID  
Sbjct: 173 HGIHIHDCKQAGNGNIRNSPHHSGWWTQSDGDGISIFASKDIWIDHNSLSNCHDGLIDAI 232

Query: 210 RGSTDITISNNWLRDQDKVILLGHDDDYFRDRNMKII-----WSTGKVENAKWKFHSVRD 269
            GST ITISNN++   DKV+LLGH D Y +D+NM++      +  G V+      H    
Sbjct: 233 HGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFH 292

Query: 270 VFENGASFAQIGAGRGGVKPN-YNALQRFPVVDAKWLRSLTSSSGA 309
           V  N  +  ++ A  G   P  Y+   RF   + ++ + +T    A
Sbjct: 293 VVNNDYTHWEMYAIGGSASPTIYSQGNRFLAPNTRFDKEVTKHENA 335

BLAST of Cp4.1LG02g10060 vs. TrEMBL
Match: A0A0A0LPZ1_CUCSA (Pectate lyase OS=Cucumis sativus GN=Csa_1G031910 PE=3 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 6.8e-116
Identity = 198/243 (81.48%), Postives = 224/243 (92.18%), Query Frame = 1

Query: 4   QLIILSLISVALL--AAVSHTEARAMNMNAIDRCWRPNPYWRKNRQKLALCSIGFVGKMT 63
           QL +LSLIS  LL   AVS++EA  +NMNAIDRCWR NPYWRKNRQ+LA CS+GF GKMT
Sbjct: 8   QLSVLSLISFILLISTAVSYSEAHGLNMNAIDRCWRSNPYWRKNRQQLATCSVGFAGKMT 67

Query: 64  NNIGRNLVHYQVTDPSDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYT 123
           NN+GRNL+HYQVTDPSDDP+ PRPGTLRYGATMIK KVWITF+KDMHIVL KPLL+SS+T
Sbjct: 68  NNMGRNLMHYQVTDPSDDPINPRPGTLRYGATMIKHKVWITFKKDMHIVLQKPLLVSSFT 127

Query: 124 AIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDA 183
           AIDGRG+TVHITGNACLMV RATN+IIHGLIIHHCK+QAAG+VMGP +K++SLG+VDGDA
Sbjct: 128 AIDGRGSTVHITGNACLMVVRATNIIIHGLIIHHCKSQAAGQVMGPNAKVISLGNVDGDA 187

Query: 184 IRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYFRDRN 243
           IRLV+ASK+WIDHNTLYRCEDGL+DVTRGSTDITISNNW RDQDKVILLGHDD Y RDR+
Sbjct: 188 IRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVRDRS 247

Query: 244 MKI 245
           M++
Sbjct: 248 MRV 250

BLAST of Cp4.1LG02g10060 vs. TrEMBL
Match: A0A067KZ75_JATCU (Pectate lyase OS=Jatropha curcas GN=JCGZ_15918 PE=3 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 9.2e-97
Identity = 160/217 (73.73%), Postives = 190/217 (87.56%), Query Frame = 1

Query: 28  NMNAIDRCWRPNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPSDDPLTPRPGT 87
           N+NAID CWR NP+WR++RQ+LA CSIGF GKM NNIG+N++ Y+VTDPSDDPL P+PGT
Sbjct: 25  NINAIDSCWRQNPHWRRSRQQLAKCSIGFAGKMINNIGKNVIRYKVTDPSDDPLNPKPGT 84

Query: 88  LRYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNVI 147
           LRYGAT+IK KVWITFQK+MHI L+KPLLISS+TA+DGRG ++HITGNACL++ + +NVI
Sbjct: 85  LRYGATLIKEKVWITFQKNMHIKLDKPLLISSFTALDGRGVSIHITGNACLLLYKVSNVI 144

Query: 148 IHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLIDV 207
           IHGL IHHCKA A G V GP  KI+ L  +DGDAIRLV+ASK+WIDHNTLY C+DGL+DV
Sbjct: 145 IHGLNIHHCKASAPGSVRGPDGKIMPLSQMDGDAIRLVTASKVWIDHNTLYACQDGLLDV 204

Query: 208 TRGSTDITISNNWLRDQDKVILLGHDDDYFRDRNMKI 245
           TRGST  TISNNW RDQDKV+LLGHDD YFRD+NMK+
Sbjct: 205 TRGSTSTTISNNWFRDQDKVMLLGHDDGYFRDKNMKV 241

BLAST of Cp4.1LG02g10060 vs. TrEMBL
Match: K7LNC8_SOYBN (Pectate lyase OS=Glycine max GN=GLYMA_11G063300 PE=3 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 3.5e-96
Identity = 160/240 (66.67%), Postives = 198/240 (82.50%), Query Frame = 1

Query: 5   LIILSLISVALLAAVSHTEARAMNMNAIDRCWRPNPYWRKNRQKLALCSIGFVGKMTNNI 64
           +I++  +    L+A   ++   + MN IDRCWR NP WR++R +LA CS+G+ GKMTNNI
Sbjct: 16  IIVIITLYTPNLSAAKQSKINGLKMNVIDRCWRLNPEWRRHRPQLATCSVGYTGKMTNNI 75

Query: 65  GRNLVHYQVTDPSDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAID 124
           G++L+HY V DPSDDP+ P+ GTLRYGA++I+ KVWITFQ+DMHI L +PLLISS+TAID
Sbjct: 76  GKDLIHYIVIDPSDDPINPKRGTLRYGASVIQGKVWITFQRDMHIKLERPLLISSFTAID 135

Query: 125 GRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRL 184
           GRG  VHI  NACLM+ +ATN+IIHG+ +HHCK QA G VMGP  K++ LGHVDGDAIRL
Sbjct: 136 GRGVNVHIANNACLMIFKATNIIIHGIRVHHCKPQAPGVVMGPEGKVIPLGHVDGDAIRL 195

Query: 185 VSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYFRDRNMKI 244
           V+ASKIWIDHNTLY C+DGL+DVTRGSTD+TISNNW RDQDKV+LLGHDD Y RD+NMK+
Sbjct: 196 VTASKIWIDHNTLYNCQDGLLDVTRGSTDVTISNNWFRDQDKVMLLGHDDGYVRDQNMKV 255

BLAST of Cp4.1LG02g10060 vs. TrEMBL
Match: A0A0B2SCK6_GLYSO (Pectate lyase OS=Glycine soja GN=glysoja_003759 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 1.3e-95
Identity = 158/216 (73.15%), Postives = 187/216 (86.57%), Query Frame = 1

Query: 29  MNAIDRCWRPNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPSDDPLTPRPGTL 88
           MN IDRCWR NP WR++R +LA CS+G+ GKMTNNIG++L+HY V DPSDDP+ P+ GTL
Sbjct: 1   MNVIDRCWRLNPEWRRHRPQLATCSVGYTGKMTNNIGKDLIHYIVIDPSDDPINPKRGTL 60

Query: 89  RYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNVII 148
           RYGA++I+ KVWITFQ+DMHI L +PLLISS+TAIDGRG  VHI  NACLM+ +ATN+II
Sbjct: 61  RYGASVIQGKVWITFQRDMHIKLERPLLISSFTAIDGRGVNVHIANNACLMIFKATNIII 120

Query: 149 HGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLIDVT 208
           HG+ +HHCK QA G VMGP  K++ LGHVDGDAIRLV+ASKIWIDHNTLY C+DGL+DVT
Sbjct: 121 HGIRVHHCKPQAPGVVMGPEGKVIPLGHVDGDAIRLVTASKIWIDHNTLYNCQDGLLDVT 180

Query: 209 RGSTDITISNNWLRDQDKVILLGHDDDYFRDRNMKI 245
           RGSTD+TISNNW RDQDKV+LLGHDD Y RD+NMKI
Sbjct: 181 RGSTDVTISNNWFRDQDKVMLLGHDDGYVRDQNMKI 216

BLAST of Cp4.1LG02g10060 vs. TrEMBL
Match: G7K488_MEDTR (Pectate lyase OS=Medicago truncatula GN=MTR_5g035840 PE=3 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 3.0e-95
Identity = 158/239 (66.11%), Postives = 197/239 (82.43%), Query Frame = 1

Query: 6   IILSLISVALLAAVSHTEARAMNMNAIDRCWRPNPYWRKNRQKLALCSIGFVGKMTNNIG 65
           I++++   +  +    ++   + MN IDRCWRPNP WRK+RQ+LA CS+G+ GKMTNNIG
Sbjct: 17  IVITIFFTSKFSFAKQSKLMGLKMNMIDRCWRPNPEWRKHRQQLATCSVGYAGKMTNNIG 76

Query: 66  RNLVHYQVTDPSDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDG 125
           + L HY+VTDP+DDP+ P+PGTLRYGA++I+ KVWITF+KDM+I L KPLLISS+T IDG
Sbjct: 77  KGLTHYKVTDPNDDPIKPQPGTLRYGASVIQGKVWITFKKDMNIKLIKPLLISSFTTIDG 136

Query: 126 RGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLV 185
           RG  VHI  NACLM+S+AT++IIH + IHHCKAQ  G VMGP  K++ LG VDGDAIRLV
Sbjct: 137 RGVNVHIADNACLMISKATDIIIHSIRIHHCKAQTPGMVMGPNGKVIHLGQVDGDAIRLV 196

Query: 186 SASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYFRDRNMKI 245
           +ASKIWIDHNTLY CEDGL+DVTRGST++T+SNNW R+QDKV+LLGHDD Y RD NMK+
Sbjct: 197 TASKIWIDHNTLYDCEDGLLDVTRGSTNVTVSNNWFREQDKVMLLGHDDGYLRDINMKV 255

BLAST of Cp4.1LG02g10060 vs. TAIR10
Match: AT5G09280.1 (AT5G09280.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 288.1 bits (736), Expect = 6.5e-78
Identity = 129/185 (69.73%), Postives = 159/185 (85.95%), Query Frame = 1

Query: 60  MTNNIGRNLVHYQVTDPSDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISS 119
           MT NIG+ +  Y+VTDPSDDPL P+PGTLRYGAT++K K WITF+++M I L+KPLLISS
Sbjct: 1   MTGNIGKGVTQYKVTDPSDDPLNPKPGTLRYGATLVKGKKWITFKRNMKIKLHKPLLISS 60

Query: 120 YTAIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDG 179
           +TA+DGRGA+VHI+G ACL+V RAT+VIIHGL IH CKA     VMGP SKI+ LGH+DG
Sbjct: 61  FTALDGRGASVHISGPACLIVYRATDVIIHGLKIHDCKAHPPSSVMGPDSKIIQLGHMDG 120

Query: 180 DAIRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYFRD 239
           DAIRLV+A K+WIDHNTLY CEDGL+DVT GSTD+T+SNNW R+QDKV+LLGHDD Y +D
Sbjct: 121 DAIRLVTAKKVWIDHNTLYDCEDGLLDVTLGSTDVTVSNNWFRNQDKVMLLGHDDGYVKD 180

Query: 240 RNMKI 245
           ++M++
Sbjct: 181 KDMRV 185

BLAST of Cp4.1LG02g10060 vs. TAIR10
Match: AT3G27400.1 (AT3G27400.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 218.4 bits (555), Expect = 6.4e-57
Identity = 122/286 (42.66%), Postives = 163/286 (56.99%), Query Frame = 1

Query: 30  NAIDRCWRPNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPS-DDPLTPRPGTL 89
           N ID CWR +P W  NRQ+LA C+IGF GK     GR    Y VTDP+ DDP+ PRPGTL
Sbjct: 64  NPIDDCWRCDPNWETNRQRLADCAIGF-GKNAIG-GRKGRIYVVTDPANDDPVNPRPGTL 123

Query: 90  RYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNVII 149
           RY  T  +P +WI F++DM I L K L+I+S+  IDGRG++VHIT   CL +  ATN+II
Sbjct: 124 RYAVTQEEP-LWIIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIII 183

Query: 150 HGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLIDVT 209
           HG+ IH CK  + G +             DGDA+ +     +WIDH +L  C+DGLID  
Sbjct: 184 HGINIHDCKPGSGGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCSLSNCDDGLIDAI 243

Query: 210 RGSTDITISNNWLRDQDKVILLGHDDDYFRDRNMKII-----WSTGKVENAKWKFHSVRD 269
            GST ITISNN +   DKV+LLGH D Y +D+NM++      +  G V+      H    
Sbjct: 244 HGSTAITISNNHMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFH 303

Query: 270 VFENGASFAQIGAGRGGVKPN-YNALQRFPVVDAKWLRSLTSSSGA 309
           V  N  +  ++ A  G   P  Y+   RF   + ++ + +T    A
Sbjct: 304 VVNNDYTHWEMYAIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDA 346

BLAST of Cp4.1LG02g10060 vs. TAIR10
Match: AT4G13210.2 (AT4G13210.2 Pectin lyase-like superfamily protein)

HSP 1 Score: 215.3 bits (547), Expect = 5.4e-56
Identity = 119/286 (41.61%), Postives = 160/286 (55.94%), Query Frame = 1

Query: 30  NAIDRCWRPNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPSD-DPLTPRPGTL 89
           N ID CWR +  W + R++LA C+IGF        GR+  +Y VTDPSD DP+TP+PGTL
Sbjct: 90  NPIDDCWRCDKKWHRRRKRLADCAIGFGRNAVG--GRDGRYYIVTDPSDHDPVTPKPGTL 149

Query: 90  RYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNVII 149
           RY     +P +WI F++DM I L++ L+++S+  IDGRG  VHI G ACL V   TN+II
Sbjct: 150 RYAVIQDEP-LWIVFKRDMVITLSQELIMNSFKTIDGRGVNVHIAGGACLTVQYVTNIII 209

Query: 150 HGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLIDVT 209
           HG+ IH CK      V    S        DGD I +  +S IWIDHN+L  C DGLID  
Sbjct: 210 HGINIHDCKRTGNAMVRSSESHYGWRTMADGDGISIFGSSHIWIDHNSLSSCADGLIDAI 269

Query: 210 RGSTDITISNNWLRDQDKVILLGHDDDYFRDRNMKII-----WSTGKVENAKWKFHSVRD 269
            GST ITISNN+L   ++ ILLGH D Y RD+ M++      +  G ++      H    
Sbjct: 270 MGSTAITISNNYLTHHNEAILLGHTDSYTRDKMMQVTIAYNHFGEGLIQRMPRCRHGYFH 329

Query: 270 VFENGASFAQIGAGRGGVKPNYNAL-QRFPVVDAKWLRSLTSSSGA 309
           V  N  +  ++ A  G   P  N+   RF     ++ + +T   GA
Sbjct: 330 VVNNDYTHWEMYAIGGSANPTINSQGNRFLAPGNRFAKEVTKRVGA 372

BLAST of Cp4.1LG02g10060 vs. TAIR10
Match: AT1G30350.1 (AT1G30350.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 213.8 bits (543), Expect = 1.6e-55
Identity = 115/250 (46.00%), Postives = 152/250 (60.80%), Query Frame = 1

Query: 1   MATQLIILSLISVALLAAVSHTE------ARAMNMNAIDRCWRPNPYWRKNRQKLALCSI 60
           MA+ ++I+SL+  A  + +  T       A  +++N ID CWR NP W  NRQ LA C++
Sbjct: 1   MASLVVIVSLLLAAFASPLLETAHSYNVTAPRVSLNPIDACWRRNPKWATNRQALAHCAV 60

Query: 61  GFVGKMTNNIGRNLVHYQVTDPSDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKP 120
           G+ GK     G+N   Y VT+PSD+P  P PGTLRY  +  KP +WITF +DM IVL   
Sbjct: 61  GY-GKAAIG-GKNGPIYVVTNPSDNPTRPSPGTLRYAVSQPKP-LWITFARDMVIVLKSQ 120

Query: 121 LLISSYTAIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSL 180
           L+I+SY  IDGRGA V I    CL + +  +VIIHG+ IH CKA   G            
Sbjct: 121 LMINSYKTIDGRGAKVEIANGPCLRIRQVKHVIIHGISIHDCKADPNG------------ 180

Query: 181 GHVDGDAIRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDD 240
             +DGD IR+  ++ +WIDH  L RC DGLIDV   ST +TISNN+    DKV+LLGHDD
Sbjct: 181 --MDGDGIRVFQSTHVWIDHCFLSRCHDGLIDVIVSSTAVTISNNYFTQHDKVMLLGHDD 233

Query: 241 DYFRDRNMKI 245
            Y  D++M++
Sbjct: 241 SYMGDKDMRV 233

BLAST of Cp4.1LG02g10060 vs. TAIR10
Match: AT3G24230.1 (AT3G24230.1 Pectate lyase family protein)

HSP 1 Score: 212.2 bits (539), Expect = 4.6e-55
Identity = 114/266 (42.86%), Postives = 152/266 (57.14%), Query Frame = 1

Query: 28  NMNAIDRCWRPNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPSDDPLT-PRPG 87
           N N ID CWR +  W KNR+ LA C +GF  K     GRN  +Y VTD SDD +  P+PG
Sbjct: 102 NGNLIDDCWRCDRNWNKNRKHLADCGMGFGSKAFG--GRNGSYYVVTDHSDDDVVNPKPG 161

Query: 88  TLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNV 147
           TLR+    ++P +WI F++DM I L + L+++S+  ID RGA VHI   AC+ +   TNV
Sbjct: 162 TLRHAVIQVEP-LWIIFKRDMVIKLKQELIMNSFKTIDARGANVHIANGACITIQNITNV 221

Query: 148 IIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLID 207
           I+HGL IH CK      V    S+    G  DGDAI +  +S IWIDHN+L  C DGL+D
Sbjct: 222 IVHGLHIHDCKRTGNVTVRSSPSQAGFRGTADGDAINIFGSSHIWIDHNSLSNCTDGLVD 281

Query: 208 VTRGSTDITISNNWLRDQDKVILLGHDDDYFRDRNMKII-----WSTGKVENAKWKFHSV 267
           V  GST ITISNN     D+V+LLGH+D Y RD+ M++      +  G ++      H  
Sbjct: 282 VVNGSTAITISNNHFTHHDEVMLLGHNDSYTRDKMMQVTVAYNHFGEGLIQRMPRCRHGY 341

Query: 268 RDVFENGASFAQIGAGRGGVKPNYNA 288
             V  N  +  ++ A  G   P  N+
Sbjct: 342 FHVVNNDYTHWKMYAIGGSANPTINS 364

BLAST of Cp4.1LG02g10060 vs. NCBI nr
Match: gi|659066329|ref|XP_008439120.1| (PREDICTED: putative pectate lyase 2 [Cucumis melo])

HSP 1 Score: 427.2 bits (1097), Expect = 2.6e-116
Identity = 200/243 (82.30%), Postives = 221/243 (90.95%), Query Frame = 1

Query: 4   QLIILSLISVALLAA--VSHTEARAMNMNAIDRCWRPNPYWRKNRQKLALCSIGFVGKMT 63
           QL +LSLIS   L +  VS +EA  ++MNAIDRCWR NPYWR+NRQ+LA CS+GF GKMT
Sbjct: 6   QLSVLSLISFIFLISNVVSSSEAHGLSMNAIDRCWRSNPYWRRNRQQLATCSVGFAGKMT 65

Query: 64  NNIGRNLVHYQVTDPSDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYT 123
           NN+GRNL+HYQVTDPSDDP+ PRPGTLRYGATMIK KVWITFQKDMHIVL KPLLISS+T
Sbjct: 66  NNMGRNLIHYQVTDPSDDPINPRPGTLRYGATMIKHKVWITFQKDMHIVLQKPLLISSFT 125

Query: 124 AIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDA 183
           AIDGRGATVHITGNACLMV RATN+IIHGLIIHHC++QAAG VMGP SK++SLGHVDGDA
Sbjct: 126 AIDGRGATVHITGNACLMVLRATNIIIHGLIIHHCRSQAAGEVMGPNSKVISLGHVDGDA 185

Query: 184 IRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYFRDRN 243
           IRLV+ASK+WIDHNTLYRCEDGL+DVTRGSTDITISNNW RDQDKVILLGHDD Y RDRN
Sbjct: 186 IRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVRDRN 245

Query: 244 MKI 245
           MK+
Sbjct: 246 MKV 248

BLAST of Cp4.1LG02g10060 vs. NCBI nr
Match: gi|700208874|gb|KGN63970.1| (hypothetical protein Csa_1G031910 [Cucumis sativus])

HSP 1 Score: 425.2 bits (1092), Expect = 9.7e-116
Identity = 198/243 (81.48%), Postives = 224/243 (92.18%), Query Frame = 1

Query: 4   QLIILSLISVALL--AAVSHTEARAMNMNAIDRCWRPNPYWRKNRQKLALCSIGFVGKMT 63
           QL +LSLIS  LL   AVS++EA  +NMNAIDRCWR NPYWRKNRQ+LA CS+GF GKMT
Sbjct: 8   QLSVLSLISFILLISTAVSYSEAHGLNMNAIDRCWRSNPYWRKNRQQLATCSVGFAGKMT 67

Query: 64  NNIGRNLVHYQVTDPSDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYT 123
           NN+GRNL+HYQVTDPSDDP+ PRPGTLRYGATMIK KVWITF+KDMHIVL KPLL+SS+T
Sbjct: 68  NNMGRNLMHYQVTDPSDDPINPRPGTLRYGATMIKHKVWITFKKDMHIVLQKPLLVSSFT 127

Query: 124 AIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDA 183
           AIDGRG+TVHITGNACLMV RATN+IIHGLIIHHCK+QAAG+VMGP +K++SLG+VDGDA
Sbjct: 128 AIDGRGSTVHITGNACLMVVRATNIIIHGLIIHHCKSQAAGQVMGPNAKVISLGNVDGDA 187

Query: 184 IRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYFRDRN 243
           IRLV+ASK+WIDHNTLYRCEDGL+DVTRGSTDITISNNW RDQDKVILLGHDD Y RDR+
Sbjct: 188 IRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVRDRS 247

Query: 244 MKI 245
           M++
Sbjct: 248 MRV 250

BLAST of Cp4.1LG02g10060 vs. NCBI nr
Match: gi|643734841|gb|KDP41511.1| (hypothetical protein JCGZ_15918 [Jatropha curcas])

HSP 1 Score: 361.7 bits (927), Expect = 1.3e-96
Identity = 160/217 (73.73%), Postives = 190/217 (87.56%), Query Frame = 1

Query: 28  NMNAIDRCWRPNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPSDDPLTPRPGT 87
           N+NAID CWR NP+WR++RQ+LA CSIGF GKM NNIG+N++ Y+VTDPSDDPL P+PGT
Sbjct: 25  NINAIDSCWRQNPHWRRSRQQLAKCSIGFAGKMINNIGKNVIRYKVTDPSDDPLNPKPGT 84

Query: 88  LRYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNVI 147
           LRYGAT+IK KVWITFQK+MHI L+KPLLISS+TA+DGRG ++HITGNACL++ + +NVI
Sbjct: 85  LRYGATLIKEKVWITFQKNMHIKLDKPLLISSFTALDGRGVSIHITGNACLLLYKVSNVI 144

Query: 148 IHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLIDV 207
           IHGL IHHCKA A G V GP  KI+ L  +DGDAIRLV+ASK+WIDHNTLY C+DGL+DV
Sbjct: 145 IHGLNIHHCKASAPGSVRGPDGKIMPLSQMDGDAIRLVTASKVWIDHNTLYACQDGLLDV 204

Query: 208 TRGSTDITISNNWLRDQDKVILLGHDDDYFRDRNMKI 245
           TRGST  TISNNW RDQDKV+LLGHDD YFRD+NMK+
Sbjct: 205 TRGSTSTTISNNWFRDQDKVMLLGHDDGYFRDKNMKV 241

BLAST of Cp4.1LG02g10060 vs. NCBI nr
Match: gi|502082100|ref|XP_004487065.1| (PREDICTED: probable pectate lyase 4 [Cicer arietinum])

HSP 1 Score: 360.1 bits (923), Expect = 3.8e-96
Identity = 161/239 (67.36%), Postives = 198/239 (82.85%), Query Frame = 1

Query: 6   IILSLISVALLAAVSHTEARAMNMNAIDRCWRPNPYWRKNRQKLALCSIGFVGKMTNNIG 65
           +++ +I     ++   ++   + MN IDRCWRPNP WR+ RQ+LA CSIG+VGKM NN+G
Sbjct: 17  VVVIVIFTPKFSSAKQSKLVGLKMNMIDRCWRPNPEWRRYRQQLAFCSIGYVGKMINNVG 76

Query: 66  RNLVHYQVTDPSDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDG 125
            +L+HY+V D +DDP+ P+PGTLRYGA++I+ KVWITFQKDM+I L KPLLISS+T IDG
Sbjct: 77  NDLIHYKVIDSNDDPINPKPGTLRYGASVIQGKVWITFQKDMNIKLMKPLLISSFTTIDG 136

Query: 126 RGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLV 185
           RGATVHI  NACLM+ + TN+IIH + IHHCKAQA G VMGP  K++SLG VDGDAIRLV
Sbjct: 137 RGATVHIADNACLMIYKTTNIIIHNIRIHHCKAQAPGMVMGPNGKVISLGQVDGDAIRLV 196

Query: 186 SASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYFRDRNMKI 245
           +ASKIWIDHNTLY CEDGL+DVTRGSTD+TISNNW R+QDKV+LLGHDD Y RD+NMK+
Sbjct: 197 TASKIWIDHNTLYSCEDGLLDVTRGSTDVTISNNWFREQDKVMLLGHDDGYVRDKNMKV 255

BLAST of Cp4.1LG02g10060 vs. NCBI nr
Match: gi|571487513|ref|XP_006590673.1| (PREDICTED: probable pectate lyase 4 [Glycine max])

HSP 1 Score: 359.8 bits (922), Expect = 5.0e-96
Identity = 160/240 (66.67%), Postives = 198/240 (82.50%), Query Frame = 1

Query: 5   LIILSLISVALLAAVSHTEARAMNMNAIDRCWRPNPYWRKNRQKLALCSIGFVGKMTNNI 64
           +I++  +    L+A   ++   + MN IDRCWR NP WR++R +LA CS+G+ GKMTNNI
Sbjct: 16  IIVIITLYTPNLSAAKQSKINGLKMNVIDRCWRLNPEWRRHRPQLATCSVGYTGKMTNNI 75

Query: 65  GRNLVHYQVTDPSDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAID 124
           G++L+HY V DPSDDP+ P+ GTLRYGA++I+ KVWITFQ+DMHI L +PLLISS+TAID
Sbjct: 76  GKDLIHYIVIDPSDDPINPKRGTLRYGASVIQGKVWITFQRDMHIKLERPLLISSFTAID 135

Query: 125 GRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRL 184
           GRG  VHI  NACLM+ +ATN+IIHG+ +HHCK QA G VMGP  K++ LGHVDGDAIRL
Sbjct: 136 GRGVNVHIANNACLMIFKATNIIIHGIRVHHCKPQAPGVVMGPEGKVIPLGHVDGDAIRL 195

Query: 185 VSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYFRDRNMKI 244
           V+ASKIWIDHNTLY C+DGL+DVTRGSTD+TISNNW RDQDKV+LLGHDD Y RD+NMK+
Sbjct: 196 VTASKIWIDHNTLYNCQDGLLDVTRGSTDVTISNNWFRDQDKVMLLGHDDGYVRDQNMKV 255

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PLY11_ARATH1.1e-5542.66Putative pectate lyase 11 OS=Arabidopsis thaliana GN=At3g27400 PE=3 SV=2[more]
PLY14_ARATH9.6e-5541.61Putative pectate lyase 14 OS=Arabidopsis thaliana GN=At4g13210 PE=2 SV=2[more]
PLY4_ARATH2.8e-5446.00Probable pectate lyase 4 OS=Arabidopsis thaliana GN=At1g30350 PE=2 SV=1[more]
PLY9_ARATH8.1e-5442.86Probable pectate lyase 9 OS=Arabidopsis thaliana GN=At3g24230 PE=2 SV=1[more]
PLY_ZINVI1.1e-5341.26Pectate lyase OS=Zinnia violacea PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LPZ1_CUCSA6.8e-11681.48Pectate lyase OS=Cucumis sativus GN=Csa_1G031910 PE=3 SV=1[more]
A0A067KZ75_JATCU9.2e-9773.73Pectate lyase OS=Jatropha curcas GN=JCGZ_15918 PE=3 SV=1[more]
K7LNC8_SOYBN3.5e-9666.67Pectate lyase OS=Glycine max GN=GLYMA_11G063300 PE=3 SV=1[more]
A0A0B2SCK6_GLYSO1.3e-9573.15Pectate lyase OS=Glycine soja GN=glysoja_003759 PE=3 SV=1[more]
G7K488_MEDTR3.0e-9566.11Pectate lyase OS=Medicago truncatula GN=MTR_5g035840 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G09280.16.5e-7869.73 Pectin lyase-like superfamily protein[more]
AT3G27400.16.4e-5742.66 Pectin lyase-like superfamily protein[more]
AT4G13210.25.4e-5641.61 Pectin lyase-like superfamily protein[more]
AT1G30350.11.6e-5546.00 Pectin lyase-like superfamily protein[more]
AT3G24230.14.6e-5542.86 Pectate lyase family protein[more]
Match NameE-valueIdentityDescription
gi|659066329|ref|XP_008439120.1|2.6e-11682.30PREDICTED: putative pectate lyase 2 [Cucumis melo][more]
gi|700208874|gb|KGN63970.1|9.7e-11681.48hypothetical protein Csa_1G031910 [Cucumis sativus][more]
gi|643734841|gb|KDP41511.1|1.3e-9673.73hypothetical protein JCGZ_15918 [Jatropha curcas][more]
gi|502082100|ref|XP_004487065.1|3.8e-9667.36PREDICTED: probable pectate lyase 4 [Cicer arietinum][more]
gi|571487513|ref|XP_006590673.1|5.0e-9666.67PREDICTED: probable pectate lyase 4 [Glycine max][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR018082AmbAllergen
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR002022Pec_lyase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043170 macromolecule metabolic process
biological_process GO:0044238 primary metabolic process
biological_process GO:0045490 pectin catabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0043167 ion binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g10060.1Cp4.1LG02g10060.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002022Pectate lyase/Amb allergenPFAMPF00544Pec_lyase_Ccoord: 118..235
score: 3.8
IPR002022Pectate lyase/Amb allergenSMARTSM00656amb_allcoord: 106..286
score: 3.2
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 29..247
score: 1.32
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 30..244
score: 1.3
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 178..199
score: 4.7E-16coord: 77..102
score: 4.7E-16coord: 114..130
score: 4.7
NoneNo IPR availablePANTHERPTHR31683FAMILY NOT NAMEDcoord: 1..317
score: 8.5E
NoneNo IPR availablePANTHERPTHR31683:SF8MAJOR POLLEN ALLERGEN-LIKE PROTEINcoord: 1..317
score: 8.5E