CmaCh13G002880 (gene) Cucurbita maxima (Rimu)

NameCmaCh13G002880
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionHistidine-containing phosphotransfer protein, putative
LocationCma_Chr13 : 2551253 .. 2552537 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGCATCGACTTCCTCATCCGCACCTGCACCCGCACCTGCCTCCGGTTCTTCAACCGCTTCGGATCATCCTCAGAGACATATGATCCGGAAGTTCATCCAGGCTTTGCGCGAACAAGTGAGATCTTCTTCAATGTTTCAATCTCTGTTCTGATTCGTGTTTCTTTGGCCGTTCTTGAGTTCAAGATTATGAGTTTCGGTGTTTCTTCTAGGGATTTCTTGACGATAACTTCAATAACCAGATGAATCTTCACGAGAATCGAAATTCCTTGGCTACATTCTGCGGTGACGCCGAACCTAAACTCGATAGAGTTGAAAAACTTCTGTAATTATTCGCCTCCTTAAACGCTTTGCATTTGATCGCTGCCTAATTCTGTTTTTCTCAAATCTTTTGAACTTTATATTTTCTAGTGCAAGAGAGACTGTGGAATTTGATACTGTGATGAACCTCATGGAATCGCTCAAGACCAATGCCGCTGGGTAACGCGCTCCACTCAATTTTACTTTTAGTGTTTATGAGAATCGAATCGCCAGTAACAATTGGAAGTTTCAAAGTCTCCTTTGCCCTAACGTAATTGTTTCTGCGGTAGAATCATGAATAAGAGTTTCTAGTGACTGTTTTCTTTCTCTTAAGGTCTTAATAATAATCATTGTTCTTTCTGTTACATTGAAAAGTGATTTTGGCGACACAATTTATTCGGAAGATTGTTATGAAAACAGCGCGTAACGAATGGACGGTGGTGTGTGTGTGTGTGTTGACAGGGTTGGTGGTAAGCGACTGTCAACTGCTTGTGCTGAGATGCAAGATTTGTGCACTGATAACAACGCTGAACCGTAATGACAAACAAGTGTTTCTTACCTTAATTTAGGCATTAATTCTTCGTATTTTGGATAATCGTCTAAAGAACATGAGGTTTTTGACTTGTTTGCAGTTTACAAGAAGCCTACAGGAAGGTCACCTGGGAATACTATGTTTTACGCGACTGCTTCCACCACATTCTGCAGGTACCACTTCACCCTTTCCTTTTCAAGGTTTAGTTACTTATTTCTTGAAAATTGGTCATGATTTTGGCGAAATTGTTGGAATTAGGCGGAGAGGGCAATCCAAATGAGCTCAGAAGAATGAAGAAACTAAGATTTGGAGTAGTAGGCTATAAATAAACGCATTTATGCGTTGGTATGGAAGGAAGGCTATTATTCCAGAAAGGCTTGCTTAGTTACTTTTTGTTTTGTATTGTAAACAAGCTTGTATGGACAAAACATATTATACTACAACAATTCTA

mRNA sequence

ATGTCTGCATCGACTTCCTCATCCGCACCTGCACCCGCACCTGCCTCCGGTTCTTCAACCGCTTCGGATCATCCTCAGAGACATATGATCCGGAAGTTCATCCAGGCTTTGCGCGAACAAGGATTTCTTGACGATAACTTCAATAACCAGATGAATCTTCACGAGAATCGAAATTCCTTGGCTACATTCTGCGGTGACGCCGAACCTAAACTCGATAGAGTTGAAAAACTTCTTGCAAGAGAGACTGTGGAATTTGATACTGTGATGAACCTCATGGAATCGCTCAAGACCAATGCCGCTGGGGTTGGTGGTAAGCGACTGTCAACTGCTTGTGCTGAGATGCAAGATTTGTGCACTGATAACAACGCTGAACCTTTACAAGAAGCCTACAGGAAGGTCACCTGGGAATACTATGTTTTACGCGACTGCTTCCACCACATTCTGCAGGCGGAGAGGGCAATCCAAATGAGCTCAGAAGAATGAAGAAACTAAGATTTGGAGTAGTAGGCTATAAATAAACGCATTTATGCGTTGGTATGGAAGGAAGGCTATTATTCCAGAAAGGCTTGCTTAGTTACTTTTTGTTTTGTATTGTAAACAAGCTTGTATGGACAAAACATATTATACTACAACAATTCTA

Coding sequence (CDS)

ATGTCTGCATCGACTTCCTCATCCGCACCTGCACCCGCACCTGCCTCCGGTTCTTCAACCGCTTCGGATCATCCTCAGAGACATATGATCCGGAAGTTCATCCAGGCTTTGCGCGAACAAGGATTTCTTGACGATAACTTCAATAACCAGATGAATCTTCACGAGAATCGAAATTCCTTGGCTACATTCTGCGGTGACGCCGAACCTAAACTCGATAGAGTTGAAAAACTTCTTGCAAGAGAGACTGTGGAATTTGATACTGTGATGAACCTCATGGAATCGCTCAAGACCAATGCCGCTGGGGTTGGTGGTAAGCGACTGTCAACTGCTTGTGCTGAGATGCAAGATTTGTGCACTGATAACAACGCTGAACCTTTACAAGAAGCCTACAGGAAGGTCACCTGGGAATACTATGTTTTACGCGACTGCTTCCACCACATTCTGCAGGCGGAGAGGGCAATCCAAATGAGCTCAGAAGAATGA

Protein sequence

MSASTSSSAPAPAPASGSSTASDHPQRHMIRKFIQALREQGFLDDNFNNQMNLHENRNSLATFCGDAEPKLDRVEKLLARETVEFDTVMNLMESLKTNAAGVGGKRLSTACAEMQDLCTDNNAEPLQEAYRKVTWEYYVLRDCFHHILQAERAIQMSSEE
BLAST of CmaCh13G002880 vs. Swiss-Prot
Match: OHP2_ORYSJ (Histidine-containing phosphotransfer protein 2 OS=Oryza sativa subsp. japonica GN=AHP2 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 8.9e-09
Identity = 35/136 (25.74%), Postives = 64/136 (47.06%), Query Frame = 1

Query: 27  RHMIRKFIQALREQGFLDDNFNNQMNLHENRNS-------LATFCGDAEPKLDRVEKLLA 86
           R  +   + ++  QG +D+ F     L +   +       +  FC DA+  ++ +  LL 
Sbjct: 7   RDQLTALLSSMFSQGLVDEQFQQLQMLQDEGGTPGFVSEVVTLFCDDADRIINEIATLLE 66

Query: 87  RETVEFDTVMNLMESLKTNAAGVGGKRLSTACAEMQDLCTDNNAEPLQEAYRKVTWEYYV 146
           +  V FD V   +  LK ++A VG +++   C + +  C D + +    A   V  ++Y 
Sbjct: 67  QPVVNFDKVDAYVHQLKGSSASVGAQKVKFTCMQFRQFCQDKSRDGCLMALAVVRNDFYD 126

Query: 147 LRDCFHHILQAERAIQ 156
           LR+ F  +LQ E+ IQ
Sbjct: 127 LRNKFQTMLQLEQQIQ 142

BLAST of CmaCh13G002880 vs. TrEMBL
Match: A0A0A0LVC3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G153540 PE=4 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 1.7e-30
Identity = 69/146 (47.26%), Postives = 104/146 (71.23%), Query Frame = 1

Query: 13  APASGSSTASDHP--QRHMIRKFIQALREQGFLDDNFNNQMNLHENRNSLATFCGDAEPK 72
           A AS SS+ S  P  Q+ MI KFI ALR+QGFLDD+FNN+     + +SL TFC ++EPK
Sbjct: 3   AAASSSSSHSQPPRSQQQMIHKFIDALRQQGFLDDDFNNRRTAPGHLDSLVTFCVESEPK 62

Query: 73  LDRVEKLLARETVEFDTVMNLMESLKTNAAGVGGKRLSTACAEMQDLCTDNNAEPLQEAY 132
           L+ ++K L  E ++F T ++  E +++ AA VGG R+++AC  +Q+   +NN    +EAY
Sbjct: 63  LEIIQKSLEGEHLDFRTAISTAEMIRSAAARVGGSRVASACIALQEHLNNNNLNGSKEAY 122

Query: 133 RKVTWEYYVLRDCFHHILQAERAIQM 157
           +K++WEYYV+RD FHH+LQAE+ +++
Sbjct: 123 KKLSWEYYVIRDSFHHMLQAEKTVEV 148

BLAST of CmaCh13G002880 vs. TrEMBL
Match: A0A0B0MPQ6_GOSAR (Histidine-containing phosphotransfer 1 OS=Gossypium arboreum GN=F383_25266 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 8.7e-11
Identity = 38/131 (29.01%), Postives = 71/131 (54.20%), Query Frame = 1

Query: 30  IRKFIQALREQGFLDDNFNNQMNLHENRNS------LATFCGDAEPKLDRVEKLLARETV 89
           ++ F+Q++ E+G LD+ F+    L ++ N       +  FC DAE  ++ + + L  + V
Sbjct: 10  LKNFVQSMFEEGVLDNQFSQIQALQDSTNPNFVEEVITLFCNDAERIINEINRNLGYQNV 69

Query: 90  EFDTVMNLMESLKTNAAGVGGKRLSTACAEMQDLCTDNNAEPLQEAYRKVTWEYYVLRDC 149
           +F  + + +  LK +++ +G  RL  ACA ++    + N E   +A + +T EY +LR  
Sbjct: 70  DFSNLDSYVHQLKGSSSSIGANRLKLACANLRQASDERNKEGCLQALQLITCEYCLLRRK 129

Query: 150 FHHILQAERAI 155
           F  +L  ER+I
Sbjct: 130 FQALLHLERSI 140

BLAST of CmaCh13G002880 vs. TrEMBL
Match: A0A0D2NTA8_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_006G156600 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.1e-10
Identity = 38/131 (29.01%), Postives = 71/131 (54.20%), Query Frame = 1

Query: 30  IRKFIQALREQGFLDDNFNNQMNLHENRNS------LATFCGDAEPKLDRVEKLLARETV 89
           ++ F+Q++ E+G LD+ F+    L ++ N       +  FC DAE  ++ + + L  + V
Sbjct: 10  LKNFVQSMFEEGVLDNQFSQIQALQDSTNPNFVEEVITLFCNDAERIVNEINRNLGYQNV 69

Query: 90  EFDTVMNLMESLKTNAAGVGGKRLSTACAEMQDLCTDNNAEPLQEAYRKVTWEYYVLRDC 149
           +F  + + +  LK +++ +G  RL  ACA ++    + N E   +A + +T EY +LR  
Sbjct: 70  DFSNLDSYVHQLKGSSSSIGANRLKLACANLRQASDERNKEGCLQALQLITCEYCLLRRK 129

Query: 150 FHHILQAERAI 155
           F  +L  ER+I
Sbjct: 130 FQALLHLERSI 140

BLAST of CmaCh13G002880 vs. TrEMBL
Match: M0RVJ3_MUSAM (Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=4 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 7.3e-10
Identity = 40/135 (29.63%), Postives = 66/135 (48.89%), Query Frame = 1

Query: 27  RHMIRKFIQALREQGFLDDNFNNQMNLHENRNS------LATFCGDAEPKLDRVEKLLAR 86
           R  +   + ++  +G LDD F     L +  +       +  FCGD+E  L  + KLL +
Sbjct: 7   REQLNGLVNSMFLEGLLDDQFEQLQMLQDESSPGFIAELITLFCGDSERILAELTKLLDQ 66

Query: 87  ETVEFDTVMNLMESLKTNAAGVGGKRLSTACAEMQDLCTDNNAEPLQEAYRKVTWEYYVL 146
             V++  V   +  LK +++ VG + +  AC + +  C ++N E    A   V  EYY+L
Sbjct: 67  AVVDYQKVDAFVHQLKGSSSSVGAQHVKLACVQFRQFCEEHNKEGCLRALNVVKHEYYLL 126

Query: 147 RDCFHHILQAERAIQ 156
           R  F  +LQ E+ IQ
Sbjct: 127 RGKFDTMLQLEQRIQ 141

BLAST of CmaCh13G002880 vs. TrEMBL
Match: A0A061ETP9_THECC (Histidine-containing phosphotransfer protein, putative OS=Theobroma cacao GN=TCM_022509 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 9.6e-10
Identity = 37/131 (28.24%), Postives = 67/131 (51.15%), Query Frame = 1

Query: 30  IRKFIQALREQGFLDDNFNNQMNLHENRNS------LATFCGDAEPKLDRVEKLLARETV 89
           ++ F+Q++ ++G LD  F+    L ++ N       +  FC DAE  +  + K L  ++V
Sbjct: 13  LKNFVQSMFDEGVLDSQFSQIQALQDSSNPNFVDEVITLFCTDAERIITELNKYLGYQSV 72

Query: 90  EFDTVMNLMESLKTNAAGVGGKRLSTACAEMQDLCTDNNAEPLQEAYRKVTWEYYVLRDC 149
           +F  +   +  LK +++ +G  RL  AC  ++    D + E    A   +T EY +LR  
Sbjct: 73  DFSKLDTYVHQLKGSSSSIGAHRLKLACVNLRQASDDRSKEGCVRALNSITREYCLLRSK 132

Query: 150 FHHILQAERAI 155
           F  ++Q ER+I
Sbjct: 133 FQTLIQLERSI 143

BLAST of CmaCh13G002880 vs. NCBI nr
Match: gi|700209813|gb|KGN64909.1| (hypothetical protein Csa_1G153540 [Cucumis sativus])

HSP 1 Score: 140.6 bits (353), Expect = 2.4e-30
Identity = 69/146 (47.26%), Postives = 104/146 (71.23%), Query Frame = 1

Query: 13  APASGSSTASDHP--QRHMIRKFIQALREQGFLDDNFNNQMNLHENRNSLATFCGDAEPK 72
           A AS SS+ S  P  Q+ MI KFI ALR+QGFLDD+FNN+     + +SL TFC ++EPK
Sbjct: 3   AAASSSSSHSQPPRSQQQMIHKFIDALRQQGFLDDDFNNRRTAPGHLDSLVTFCVESEPK 62

Query: 73  LDRVEKLLARETVEFDTVMNLMESLKTNAAGVGGKRLSTACAEMQDLCTDNNAEPLQEAY 132
           L+ ++K L  E ++F T ++  E +++ AA VGG R+++AC  +Q+   +NN    +EAY
Sbjct: 63  LEIIQKSLEGEHLDFRTAISTAEMIRSAAARVGGSRVASACIALQEHLNNNNLNGSKEAY 122

Query: 133 RKVTWEYYVLRDCFHHILQAERAIQM 157
           +K++WEYYV+RD FHH+LQAE+ +++
Sbjct: 123 KKLSWEYYVIRDSFHHMLQAEKTVEV 148

BLAST of CmaCh13G002880 vs. NCBI nr
Match: gi|659071740|ref|XP_008461693.1| (PREDICTED: histidine-containing phosphotransfer protein 5-like [Cucumis melo])

HSP 1 Score: 138.7 bits (348), Expect = 9.2e-30
Identity = 71/156 (45.51%), Postives = 105/156 (67.31%), Query Frame = 1

Query: 1   MSASTSSSAPAPAPASGSSTASDHPQRHMIRKFIQALREQGFLDDNFNNQMNLHENRNSL 60
           MSA+ SSS+  P P           Q+ MI KFI ALR+QGFLD++FNN+     + +SL
Sbjct: 1   MSAAASSSSSHPQPPRS--------QQQMIHKFIDALRQQGFLDNDFNNRRTAPGHLDSL 60

Query: 61  ATFCGDAEPKLDRVEKLLARETVEFDTVMNLMESLKTNAAGVGGKRLSTACAEMQDLCTD 120
            TFC ++EPKL+ ++K L  E V+F T +N  E +++ A  VGG R+++AC  +Q+   +
Sbjct: 61  VTFCVESEPKLEIIQKALEGEHVDFRTAINTSEMIRSAATRVGGSRVASACIALQEHLNN 120

Query: 121 NNAEPLQEAYRKVTWEYYVLRDCFHHILQAERAIQM 157
           NN    +EAY+K++WEYYV+RD FHHILQAE+ +++
Sbjct: 121 NNLNGSKEAYKKLSWEYYVIRDSFHHILQAEKTVEV 148

BLAST of CmaCh13G002880 vs. NCBI nr
Match: gi|823258156|ref|XP_012461782.1| (PREDICTED: histidine-containing phosphotransfer protein 1-like [Gossypium raimondii])

HSP 1 Score: 75.1 bits (183), Expect = 1.2e-10
Identity = 43/145 (29.66%), Postives = 76/145 (52.41%), Query Frame = 1

Query: 16  SGSSTASDHPQRHMIRKFIQALREQGFLDDNFNNQMNLHENRNS------LATFCGDAEP 75
           +GSST  D  Q+  +  +I+ + +QG LDDNF+  + L   +N       ++ F  DAE 
Sbjct: 2   AGSST--DPTQQ--LNDYIRTMHDQGILDDNFDQLLALRREQNPRFITELISMFTNDAEA 61

Query: 76  KLDRVEKLLARETVEFDTVMNLMESLKTNAAGVGGKRLSTACAEMQDLCTDNNAEPLQEA 135
            +  +   L+   V+F T+++ +  LK + +G+GG R+  AC E++    D + +   E 
Sbjct: 62  YIAEITNALSLTEVDFSTIISKLHQLKGSTSGIGGHRMYQACCELRVAADDGDKDRCDEL 121

Query: 136 YRKVTWEYYVLRDCFHHILQAERAI 155
           + +V  E+  L+ CF  I +  R I
Sbjct: 122 FLRVKEEFNTLKQCFDSISEMRRNI 142

BLAST of CmaCh13G002880 vs. NCBI nr
Match: gi|728816970|gb|KHG02307.1| (Histidine-containing phosphotransfer 1 [Gossypium arboreum])

HSP 1 Score: 75.1 bits (183), Expect = 1.2e-10
Identity = 38/131 (29.01%), Postives = 71/131 (54.20%), Query Frame = 1

Query: 30  IRKFIQALREQGFLDDNFNNQMNLHENRNS------LATFCGDAEPKLDRVEKLLARETV 89
           ++ F+Q++ E+G LD+ F+    L ++ N       +  FC DAE  ++ + + L  + V
Sbjct: 10  LKNFVQSMFEEGVLDNQFSQIQALQDSTNPNFVEEVITLFCNDAERIINEINRNLGYQNV 69

Query: 90  EFDTVMNLMESLKTNAAGVGGKRLSTACAEMQDLCTDNNAEPLQEAYRKVTWEYYVLRDC 149
           +F  + + +  LK +++ +G  RL  ACA ++    + N E   +A + +T EY +LR  
Sbjct: 70  DFSNLDSYVHQLKGSSSSIGANRLKLACANLRQASDERNKEGCLQALQLITCEYCLLRRK 129

Query: 150 FHHILQAERAI 155
           F  +L  ER+I
Sbjct: 130 FQALLHLERSI 140

BLAST of CmaCh13G002880 vs. NCBI nr
Match: gi|823174569|ref|XP_012485821.1| (PREDICTED: histidine-containing phosphotransfer protein 1-like [Gossypium raimondii])

HSP 1 Score: 74.7 bits (182), Expect = 1.6e-10
Identity = 38/131 (29.01%), Postives = 71/131 (54.20%), Query Frame = 1

Query: 30  IRKFIQALREQGFLDDNFNNQMNLHENRNS------LATFCGDAEPKLDRVEKLLARETV 89
           ++ F+Q++ E+G LD+ F+    L ++ N       +  FC DAE  ++ + + L  + V
Sbjct: 10  LKNFVQSMFEEGVLDNQFSQIQALQDSTNPNFVEEVITLFCNDAERIVNEINRNLGYQNV 69

Query: 90  EFDTVMNLMESLKTNAAGVGGKRLSTACAEMQDLCTDNNAEPLQEAYRKVTWEYYVLRDC 149
           +F  + + +  LK +++ +G  RL  ACA ++    + N E   +A + +T EY +LR  
Sbjct: 70  DFSNLDSYVHQLKGSSSSIGANRLKLACANLRQASDERNKEGCLQALQLITCEYCLLRRK 129

Query: 150 FHHILQAERAI 155
           F  +L  ER+I
Sbjct: 130 FQALLHLERSI 140

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
OHP2_ORYSJ8.9e-0925.74Histidine-containing phosphotransfer protein 2 OS=Oryza sativa subsp. japonica G... [more]
Match NameE-valueIdentityDescription
A0A0A0LVC3_CUCSA1.7e-3047.26Uncharacterized protein OS=Cucumis sativus GN=Csa_1G153540 PE=4 SV=1[more]
A0A0B0MPQ6_GOSAR8.7e-1129.01Histidine-containing phosphotransfer 1 OS=Gossypium arboreum GN=F383_25266 PE=4 ... [more]
A0A0D2NTA8_GOSRA1.1e-1029.01Uncharacterized protein OS=Gossypium raimondii GN=B456_006G156600 PE=4 SV=1[more]
M0RVJ3_MUSAM7.3e-1029.63Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=4 SV=1[more]
A0A061ETP9_THECC9.6e-1028.24Histidine-containing phosphotransfer protein, putative OS=Theobroma cacao GN=TCM... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
gi|700209813|gb|KGN64909.1|2.4e-3047.26hypothetical protein Csa_1G153540 [Cucumis sativus][more]
gi|659071740|ref|XP_008461693.1|9.2e-3045.51PREDICTED: histidine-containing phosphotransfer protein 5-like [Cucumis melo][more]
gi|823258156|ref|XP_012461782.1|1.2e-1029.66PREDICTED: histidine-containing phosphotransfer protein 1-like [Gossypium raimon... [more]
gi|728816970|gb|KHG02307.1|1.2e-1029.01Histidine-containing phosphotransfer 1 [Gossypium arboreum][more]
gi|823174569|ref|XP_012485821.1|1.6e-1029.01PREDICTED: histidine-containing phosphotransfer protein 1-like [Gossypium raimon... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR008207Sig_transdc_His_kin_Hpt_dom
Vocabulary: Biological Process
TermDefinition
GO:0000160phosphorelay signal transduction system
Vocabulary: Molecular Function
TermDefinition
GO:0004871signal transducer activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000160 phosphorelay signal transduction system
cellular_component GO:0005575 cellular_component
cellular_component GO:0005622 intracellular
molecular_function GO:0004871 signal transducer activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh13G002880.1CmaCh13G002880.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008207Signal transduction histidine kinase, phosphotransfer (Hpt) domainGENE3DG3DSA:1.20.120.160coord: 59..148
score: 3.
IPR008207Signal transduction histidine kinase, phosphotransfer (Hpt) domainPFAMPF01627Hptcoord: 60..129
score: 9.
IPR008207Signal transduction histidine kinase, phosphotransfer (Hpt) domainunknownSSF47226Histidine-containing phosphotransfer domain, HPT domaincoord: 36..152
score: 1.31
NoneNo IPR availablePANTHERPTHR28242FAMILY NOT NAMEDcoord: 30..160
score: 1.5
NoneNo IPR availablePANTHERPTHR28242:SF10SUBFAMILY NOT NAMEDcoord: 30..160
score: 1.5