Bhi08G000063 (gene) Wax gourd

NameBhi08G000063
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionHistidine-containing phosphotransfer 1
Locationchr8 : 2767151 .. 2768376 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCACCGAGTTTCTTCAGAGCAAAGAGTTGTGATTTTCAGAAACCAAAAGCTTCGAAGAACAATTCATCGATCGAAAGAGAAACTTCTCCCAATGTCGGCGTCGGCGTCTTCCTCTTCCTCTCGGCCACCGGCGGGGATGCTTCATCCTCATAGATTCATGATCGAGAAATTCATCGACGCTTTGCGCGAACAGGTAGATCATCTTCTTCCAATTCAGCCTCTGTTTTTCACGTCTCTCTGTTCCGGATTCGTTTTTTCTTTTCCCTTTCTTGAGTTCTCAGTCGTTTCTTATAGGGATTCCTTGACGAAGACTTCAATAAGCGGAGAAATCTCCCTGAGAATCAAGATTCCTTGGCTAGATTCTGCGGTGAAGCCGAACCTAAGCTCGAAACTATTGAGAAAGCTCTGTAATGATTCAAATCCTTAATTCAGATCTTGTACTGTATCACTACTTTCTTTCAATTTCTCTTATGAATCGTACGTTTTGATTATTTTAGGGCGAGTGAGAATGTGGATTTTCGTAGCGTGATTAACACCGTGGAAATGATCAGATCTGGTGCCGCAAGGTAAGGCCGACAATTCAATTTCAATTTCATGTTATGAGATCTTGAAATTCTTGTTTCAATTTCGTTTCGAAAAAGTGAATTGGAGGATAATTTGAAAGATTCTTTCTAATTATGAACTTAAAACAGAGTTGGTGGAACTCGAGTAGCAAGTGCTTGTACTGATCTGCAACAACATTGCAATAGCAATAACATTGAGGGGTAATTAAGTGTGTTTAATTAACATAATTAATTATTTAAACAACATGAACAGATTATTAATTATCGTTAATTACTTTGTATACTTTGCCAGTTCCAATGCTGCTTACAAGAAGCTCTCCTGGGAATATTATGTTCTACGTGATTGTTTCCATCACATTCTTCAGGTAAATTTTATTATACCCTTTAATTTTCTCTTTTGAATATCAATGATTTTCTTGAAATTTACAAAGATTTTAATTAATTTTTTTTGGTAATTTGTTTGTTTAGGCTGAGAGGACATTGGAAGATTAAGAGACTAATGAAGACATCAAGATTTTAGGGAATAAATAAAGCTTGTTTAGTTAATTTTTTTTTTTGTATTATATATATTTTTTAAACAAGCTTGTTTTGGAACAAACATTTACGAGAAATTGTAATTGAAATGCACTTTTTGAAATTCTAGTGATTTCTTTGTATTCTA

mRNA sequence

CTCCACCGAGTTTCTTCAGAGCAAAGAGTTGTGATTTTCAGAAACCAAAAGCTTCGAAGAACAATTCATCGATCGAAAGAGAAACTTCTCCCAATGTCGGCGTCGGCGTCTTCCTCTTCCTCTCGGCCACCGGCGGGGATGCTTCATCCTCATAGATTCATGATCGAGAAATTCATCGACGCTTTGCGCGAACAGGGATTCCTTGACGAAGACTTCAATAAGCGGAGAAATCTCCCTGAGAATCAAGATTCCTTGGCTAGATTCTGCGGTGAAGCCGAACCTAAGCTCGAAACTATTGAGAAAGCTCTGGCGAGTGAGAATGTGGATTTTCGTAGCGTGATTAACACCGTGGAAATGATCAGATCTGGTGCCGCAAGAGTTGGTGGAACTCGAGTAGCAAGTGCTTGTACTGATCTGCAACAACATTGCAATAGCAATAACATTGAGGGTTCCAATGCTGCTTACAAGAAGCTCTCCTGGGAATATTATGTTCTACGTGATTGTTTCCATCACATTCTTCAGGCTGAGAGGACATTGGAAGATTAAGAGACTAATGAAGACATCAAGATTTTAGGGAATAAATAAAGCTTGTTTAGTTAATTTTTTTTTTTGTATTATATATATTTTTTAAACAAGCTTGTTTTGGAACAAACATTTACGAGAAATTGTAATTGAAATGCACTTTTTGAAATTCTAGTGATTTCTTTGTATTCTA

Coding sequence (CDS)

ATGTCGGCGTCGGCGTCTTCCTCTTCCTCTCGGCCACCGGCGGGGATGCTTCATCCTCATAGATTCATGATCGAGAAATTCATCGACGCTTTGCGCGAACAGGGATTCCTTGACGAAGACTTCAATAAGCGGAGAAATCTCCCTGAGAATCAAGATTCCTTGGCTAGATTCTGCGGTGAAGCCGAACCTAAGCTCGAAACTATTGAGAAAGCTCTGGCGAGTGAGAATGTGGATTTTCGTAGCGTGATTAACACCGTGGAAATGATCAGATCTGGTGCCGCAAGAGTTGGTGGAACTCGAGTAGCAAGTGCTTGTACTGATCTGCAACAACATTGCAATAGCAATAACATTGAGGGTTCCAATGCTGCTTACAAGAAGCTCTCCTGGGAATATTATGTTCTACGTGATTGTTTCCATCACATTCTTCAGGCTGAGAGGACATTGGAAGATTAA

Protein sequence

MSASASSSSSRPPAGMLHPHRFMIEKFIDALREQGFLDEDFNKRRNLPENQDSLARFCGEAEPKLETIEKALASENVDFRSVINTVEMIRSGAARVGGTRVASACTDLQQHCNSNNIEGSNAAYKKLSWEYYVLRDCFHHILQAERTLED
BLAST of Bhi08G000063 vs. TrEMBL
Match: tr|A0A1S3CFS8|A0A1S3CFS8_CUCME (histidine-containing phosphotransfer protein 5-like OS=Cucumis melo OX=3656 GN=LOC103500231 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.5e-44
Identity = 90/127 (70.87%), Postives = 103/127 (81.10%), Query Frame = 0

Query: 23  MIEKFIDALREQGFLDEDFNKRRNLPENQDSLARFCGEAEPKLETIEKALASENVDFRSV 82
           MI KFIDALR+QGFLD DFN RR  P + DSL  FC E+EPKLE I+KAL  E+VDFR+ 
Sbjct: 21  MIHKFIDALRQQGFLDNDFNNRRTAPGHLDSLVTFCVESEPKLEIIQKALEGEHVDFRTA 80

Query: 83  INTVEMIRSGAARVGGTRVASACTDLQQHCNSNNIEGSNAAYKKLSWEYYVLRDCFHHIL 142
           INT EMIRS A RVGG+RVASAC  LQ+H N+NN+ GS  AYKKLSWEYYV+RD FHHIL
Sbjct: 81  INTSEMIRSAATRVGGSRVASACIALQEHLNNNNLNGSKEAYKKLSWEYYVIRDSFHHIL 140

Query: 143 QAERTLE 150
           QAE+T+E
Sbjct: 141 QAEKTVE 147

BLAST of Bhi08G000063 vs. TrEMBL
Match: tr|A0A0A0LVC3|A0A0A0LVC3_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G153540 PE=4 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 6.7e-43
Identity = 86/126 (68.25%), Postives = 104/126 (82.54%), Query Frame = 0

Query: 24  IEKFIDALREQGFLDEDFNKRRNLPENQDSLARFCGEAEPKLETIEKALASENVDFRSVI 83
           I KFIDALR+QGFLD+DFN RR  P + DSL  FC E+EPKLE I+K+L  E++DFR+ I
Sbjct: 22  IHKFIDALRQQGFLDDDFNNRRTAPGHLDSLVTFCVESEPKLEIIQKSLEGEHLDFRTAI 81

Query: 84  NTVEMIRSGAARVGGTRVASACTDLQQHCNSNNIEGSNAAYKKLSWEYYVLRDCFHHILQ 143
           +T EMIRS AARVGG+RVASAC  LQ+H N+NN+ GS  AYKKLSWEYYV+RD FHH+LQ
Sbjct: 82  STAEMIRSAAARVGGSRVASACIALQEHLNNNNLNGSKEAYKKLSWEYYVIRDSFHHMLQ 141

Query: 144 AERTLE 150
           AE+T+E
Sbjct: 142 AEKTVE 147

BLAST of Bhi08G000063 vs. TrEMBL
Match: tr|U5GFZ5|U5GFZ5_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_004G185900v3 PE=4 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.8e-11
Identity = 42/127 (33.07%), Postives = 72/127 (56.69%), Query Frame = 0

Query: 27  FIDALREQGFLDEDFNKRRNLPENQ-----DSLARFCGEAEPKLETIEKALASENVDFRS 86
           F+ +L EQG LD  F++  +LP        D ++ FC +AE  +  + + L   ++++  
Sbjct: 13  FVKSLHEQGILDSHFDEILDLPRESPQFVIDLVSTFCSDAENAIAALIRYLNEPDINYSR 72

Query: 87  VINTVEMIRSGAARVGGTRVASACTDLQQHCNSNNIEGSNAAYKKLSWEYYVLRDCFHHI 146
           VI+ V  IR  ++ +GG R+A AC +L+  C   + +   AA+ K   EY +L++ F+ I
Sbjct: 73  VIDQVHQIRGASSCIGGHRMALACRELRYACEDKDKDRCLAAFGKTKDEYQILKEKFNII 132

Query: 147 LQAERTL 149
           LQ ERT+
Sbjct: 133 LQMERTM 139

BLAST of Bhi08G000063 vs. TrEMBL
Match: tr|A0A2I4GXM1|A0A2I4GXM1_9ROSI (histidine-containing phosphotransfer protein 2-like OS=Juglans regia OX=51240 GN=LOC109011782 PE=4 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 3.0e-11
Identity = 42/134 (31.34%), Postives = 73/134 (54.48%), Query Frame = 0

Query: 21  RFMIEKFIDALREQGFLDEDFNKRRNLPEN------QDSLARFCGEAEPKLETIEKALAS 80
           R  +  +I +  EQG LDE F++ +NL  +       +  + FC +AE  +  + + LA 
Sbjct: 7   RQQLMNYIQSSHEQGILDEAFDRVKNLENDGNPQFLSEIFSMFCHDAENGIAELTRFLAE 66

Query: 81  ENVDFRSVINTVEMIRSGAARVGGTRVASACTDLQQHCNSNNIEGSNAAYKKLSWEYYVL 140
             +D+ +V      ++  ++ VGG R+  AC +L+Q  N+   EGS  A+ K+  EY +L
Sbjct: 67  PVIDYENVSAFAHQLKGSSSSVGGHRMVLACCELRQASNNKIKEGSFEAFDKVKREYCIL 126

Query: 141 RDCFHHILQAERTL 149
           RD  ++I Q E+T+
Sbjct: 127 RDSLNYIAQMEKTI 140

BLAST of Bhi08G000063 vs. TrEMBL
Match: tr|A0A2N9FK94|A0A2N9FK94_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS15352 PE=4 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 9.7e-10
Identity = 40/134 (29.85%), Postives = 73/134 (54.48%), Query Frame = 0

Query: 21  RFMIEKFIDALREQGFLDEDFNKRRNLPENQ------DSLARFCGEAEPKLETIEKALAS 80
           R  ++ +I+++ EQG LD +F+  ++L   +      + +  FC +AE  +  + + L  
Sbjct: 7   RQQLQNYIESMHEQGILDHNFHNVQDLQNEENPHFVLEVVTMFCNDAENGIAQLARYLDE 66

Query: 81  ENVDFRSVINTVEMIRSGAARVGGTRVASACTDLQQHCNSNNIEGSNAAYKKLSWEYYVL 140
             VD+  V      +R  ++ VGG R+A AC +L++  + NN EG   A  ++  EY +L
Sbjct: 67  LVVDYPKVTGLSHQLRGSSSSVGGHRMALACRELRRASDVNNKEGCFEALGRVRHEYRIL 126

Query: 141 RDCFHHILQAERTL 149
           RD  ++I Q ER++
Sbjct: 127 RDNLNNISQMERSI 140

BLAST of Bhi08G000063 vs. NCBI nr
Match: XP_008461693.1 (PREDICTED: histidine-containing phosphotransfer protein 5-like [Cucumis melo])

HSP 1 Score: 186.8 bits (473), Expect = 5.3e-44
Identity = 90/127 (70.87%), Postives = 103/127 (81.10%), Query Frame = 0

Query: 23  MIEKFIDALREQGFLDEDFNKRRNLPENQDSLARFCGEAEPKLETIEKALASENVDFRSV 82
           MI KFIDALR+QGFLD DFN RR  P + DSL  FC E+EPKLE I+KAL  E+VDFR+ 
Sbjct: 21  MIHKFIDALRQQGFLDNDFNNRRTAPGHLDSLVTFCVESEPKLEIIQKALEGEHVDFRTA 80

Query: 83  INTVEMIRSGAARVGGTRVASACTDLQQHCNSNNIEGSNAAYKKLSWEYYVLRDCFHHIL 142
           INT EMIRS A RVGG+RVASAC  LQ+H N+NN+ GS  AYKKLSWEYYV+RD FHHIL
Sbjct: 81  INTSEMIRSAATRVGGSRVASACIALQEHLNNNNLNGSKEAYKKLSWEYYVIRDSFHHIL 140

Query: 143 QAERTLE 150
           QAE+T+E
Sbjct: 141 QAEKTVE 147

BLAST of Bhi08G000063 vs. NCBI nr
Match: KGN64909.1 (hypothetical protein Csa_1G153540 [Cucumis sativus])

HSP 1 Score: 182.6 bits (462), Expect = 1.0e-42
Identity = 86/126 (68.25%), Postives = 104/126 (82.54%), Query Frame = 0

Query: 24  IEKFIDALREQGFLDEDFNKRRNLPENQDSLARFCGEAEPKLETIEKALASENVDFRSVI 83
           I KFIDALR+QGFLD+DFN RR  P + DSL  FC E+EPKLE I+K+L  E++DFR+ I
Sbjct: 22  IHKFIDALRQQGFLDDDFNNRRTAPGHLDSLVTFCVESEPKLEIIQKSLEGEHLDFRTAI 81

Query: 84  NTVEMIRSGAARVGGTRVASACTDLQQHCNSNNIEGSNAAYKKLSWEYYVLRDCFHHILQ 143
           +T EMIRS AARVGG+RVASAC  LQ+H N+NN+ GS  AYKKLSWEYYV+RD FHH+LQ
Sbjct: 82  STAEMIRSAAARVGGSRVASACIALQEHLNNNNLNGSKEAYKKLSWEYYVIRDSFHHMLQ 141

Query: 144 AERTLE 150
           AE+T+E
Sbjct: 142 AEKTVE 147

BLAST of Bhi08G000063 vs. NCBI nr
Match: XP_022970357.1 (histidine-containing phosphotransfer protein 2-like [Cucurbita maxima])

HSP 1 Score: 172.9 bits (437), Expect = 8.0e-40
Identity = 78/132 (59.09%), Postives = 103/132 (78.03%), Query Frame = 0

Query: 18  HPHRFMIEKFIDALREQGFLDEDFNKRRNLPENQDSLARFCGEAEPKLETIEKALASENV 77
           HP R MI KFI ALREQGFLD++FN + NL EN++SLA FCG+AEPKL+ +EK LA E V
Sbjct: 24  HPQRHMIRKFIQALREQGFLDDNFNNQMNLHENRNSLATFCGDAEPKLDRVEKLLARETV 83

Query: 78  DFRSVINTVEMIRSGAARVGGTRVASACTDLQQHCNSNNIEGSNAAYKKLSWEYYVLRDC 137
           +F +V+N +E +++ AA VGG R+++AC ++Q  C  NN E    AY+K++WEYYVLRDC
Sbjct: 84  EFDTVMNLMESLKTNAAGVGGKRLSTACAEMQDLCTDNNAEPLQEAYRKVTWEYYVLRDC 143

Query: 138 FHHILQAERTLE 150
           FHHILQAER ++
Sbjct: 144 FHHILQAERAIQ 155

BLAST of Bhi08G000063 vs. NCBI nr
Match: XP_022930050.1 (histidine-containing phosphotransfer protein 2-like [Cucurbita moschata])

HSP 1 Score: 172.6 bits (436), Expect = 1.0e-39
Identity = 79/132 (59.85%), Postives = 102/132 (77.27%), Query Frame = 0

Query: 18  HPHRFMIEKFIDALREQGFLDEDFNKRRNLPENQDSLARFCGEAEPKLETIEKALASENV 77
           HP R MI KFI ALREQGFLD++FN + NL EN++SLA FCG+AEPKL+ +EK LA E V
Sbjct: 56  HPQRHMIRKFIQALREQGFLDDNFNNQMNLHENRNSLATFCGDAEPKLDRVEKLLARETV 115

Query: 78  DFRSVINTVEMIRSGAARVGGTRVASACTDLQQHCNSNNIEGSNAAYKKLSWEYYVLRDC 137
           +F +V N +E +++ AA VGG R+++AC D+Q  C  NN E    AY+K++WEYYVLRDC
Sbjct: 116 EFDTVSNLMESLKTKAAGVGGKRLSTACADMQDLCTDNNAEPLQEAYRKVTWEYYVLRDC 175

Query: 138 FHHILQAERTLE 150
           FHHILQAER ++
Sbjct: 176 FHHILQAERAIQ 187

BLAST of Bhi08G000063 vs. NCBI nr
Match: XP_022969555.1 (histidine-containing phosphotransfer protein 1-like [Cucurbita maxima])

HSP 1 Score: 172.6 bits (436), Expect = 1.0e-39
Identity = 75/131 (57.25%), Postives = 105/131 (80.15%), Query Frame = 0

Query: 18  HPHRFMIEKFIDALREQGFLDEDFNKRRNLPENQDSLARFCGEAEPKLETIEKALASENV 77
           HP R M+ KFI+ALR+QGFLDE+F+   NL +N++ L+ FC +AEPKL+ +EK+L +ENV
Sbjct: 6   HPQRHMLNKFINALRDQGFLDENFSSLGNLYDNRNMLSLFCDDAEPKLDRVEKSLKNENV 65

Query: 78  DFRSVINTVEMIRSGAARVGGTRVASACTDLQQHCNSNNIEGSNAAYKKLSWEYYVLRDC 137
           DF+ ++  +E I+  AAR+GG RVA AC +LQ+ C ++++E  N AYKK++WEYY+LRDC
Sbjct: 66  DFQGLVTIIEQIKGNAARLGGKRVAEACAELQRFCGTSDVERCNEAYKKVNWEYYILRDC 125

Query: 138 FHHILQAERTL 149
           FHHILQAERT+
Sbjct: 126 FHHILQAERTI 136

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
tr|A0A1S3CFS8|A0A1S3CFS8_CUCME3.5e-4470.87histidine-containing phosphotransfer protein 5-like OS=Cucumis melo OX=3656 GN=L... [more]
tr|A0A0A0LVC3|A0A0A0LVC3_CUCSA6.7e-4368.25Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G153540 PE=4 SV=1[more]
tr|U5GFZ5|U5GFZ5_POPTR1.8e-1133.07Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_004G185900v3 PE=... [more]
tr|A0A2I4GXM1|A0A2I4GXM1_9ROSI3.0e-1131.34histidine-containing phosphotransfer protein 2-like OS=Juglans regia OX=51240 GN... [more]
tr|A0A2N9FK94|A0A2N9FK94_FAGSY9.7e-1029.85Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS15352 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_008461693.15.3e-4470.87PREDICTED: histidine-containing phosphotransfer protein 5-like [Cucumis melo][more]
KGN64909.11.0e-4268.25hypothetical protein Csa_1G153540 [Cucumis sativus][more]
XP_022970357.18.0e-4059.09histidine-containing phosphotransfer protein 2-like [Cucurbita maxima][more]
XP_022930050.11.0e-3959.85histidine-containing phosphotransfer protein 2-like [Cucurbita moschata][more]
XP_022969555.11.0e-3957.25histidine-containing phosphotransfer protein 1-like [Cucurbita maxima][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0000160phosphorelay signal transduction system
Vocabulary: INTERPRO
TermDefinition
IPR008207Sig_transdc_His_kin_Hpt_dom
IPR036641HPT_dom_sf
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009736 cytokinin-activated signaling pathway
biological_process GO:0000160 phosphorelay signal transduction system
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0005622 intracellular
cellular_component GO:0044424 intracellular part
cellular_component GO:0005575 cellular_component
molecular_function GO:0009927 histidine phosphotransfer kinase activity
molecular_function GO:0043424 protein histidine kinase binding
molecular_function GO:0004871 signal transducer activity
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi08M000063Bhi08M000063mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036641HPT domain superfamilyGENE3DG3DSA:1.20.120.160coord: 21..150
e-value: 6.8E-21
score: 76.6
IPR036641HPT domain superfamilySUPERFAMILYSSF47226Histidine-containing phosphotransfer domain, HPT domaincoord: 40..146
NoneNo IPR availablePANTHERPTHR28242:SF5HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN 1coord: 25..147
NoneNo IPR availablePANTHERPTHR28242FAMILY NOT NAMEDcoord: 25..147
IPR008207Signal transduction histidine kinase, phosphotransfer (Hpt) domainPROSITEPS50894HPTcoord: 46..150
score: 10.907