CmaCh04G027420 (gene) Cucurbita maxima (Rimu)

Overview
NameCmaCh04G027420
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionCLAVATA3/ESR (CLE)-related protein 25
LocationCma_Chr04 : 18441999 .. 18444734 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTCGGTGGATTGGAAAGCCCTAATATTCACATCCATGAACGTGGGAGAGAGTCCTAAACATGCAAGGACCTCGTATTCTCACATGAATGCAGAATCAATTTACAGTAATCAGAATTCTTGTAACCAGACATGCGAAGCTGCCTGCCTCATGAAATTACAAAGAAAATGGTTCTTATGGGCATCAATCTACTTTGGTATCAGATACTTGTTTCTTAGTTGAATTGCTTTGATTTTCCTTCAGTATTGATTTGAGCCAGATTAGTTCATTGTGTACTGTGGGCTTGCGACAGCAGCGCATCTAAACAAATGGTAGTATTCATTTTTCATTGTTCTCTGATAGCAAATCTAAACAAATGGTAGTATTCATTTTTCATTGTTCTCTGATAGCAATTGCTCGTATGTTTTTTTCCTAATCAAGGTTTAAAATCAAGGGTTTCCTTGCAATAATTGGTCGCTGATCAACTACCAGACACTCAAAACCAACTGCCTCAATTTTCTTCAGGTGAGCAATCATTTCTCTGATGACTATGTAACATTTGGCCTAATTGGGATTAACACTTTATCTTCTTCGCAAATTTTAGTTTAATATTCGGAGATTTGGCTATGCCTCGTCTTTACGAACCTGATGTTAAGTGCTCTGAGCTTATGAGATAGTGAGATCCCCCAATTCCCCTTTTGTAGATATAGGAAAATGCAGAAACTAAGGCGAAGAAAAAGGAGCTGATGGACACATTCTTCCACAATAAAGTGACATAAATAAATACTTTTCTGCGTTTAAATCAGATTCCATCTCTCTCTTCCCACGAGAACGCAAAGTACAGTTTCACTGATACCGTCTTTATATACAAACAAAACAGCCTTACAACCCGCCAATACCTCAGCCAACCGAATTAGTTTTCCATCTCTCTTCTTTCTTCTTTCTTAGTCTATAGTTATCCATCTTCACTTCCATGCCCTGAAAATGTCTGATTTTAGTCATTTCTGTGTTTCTGTTCTGAATGGATGATGACCATTGTTGGTCAATTGGAAGCTGTGAGTTTAGGAAAAAGGTTTGGGTGTAATGGAACCTAACTGTAGATTTTTTAGGCTTCTGTTTGGAGTTTTTCTCTTTCTTGGTATCATCTGGTTTTCTTTCATAGGAGGGATTGATAATGCTAGCCCAGCATCCAGAAGAGCGACAGACATGCAGTGGAAGGTGATTGGAAGCGAGAAGCATGTCTTTCATTGGCATTCTGATTTCTATTCTGTCAGCAAGAGAAGAGTACCAAATGGACCTGATCCAATCCATAACAGGTACGCAATTCTACTTGATCTAATATATTCAAATTGCAGATATGCTTGCTTATTTTCTTTTTCTTCTGCTCTTTGCAAGTATCTTCTGAACTATTCGTCTGTTCTCCTTCCTTTCTTTTATATTTTTTATCTCCTTCATTTTGTGGGTTTTGACGCTTTGGTGTAGAATTCTTGAAATTTTTGTCATCTTGAGGACAGAAATTTGACCATTGAATTTTGTCCCTAAAAAAAAGGCAAATTAACGCGTCTGAAGGTGACCACCCTACTTCAATTAAAAGAAAGATAAGGAACTGAGAGTTCTATTACCATTAGTGAATTTAGGAAGCTACTCTATTGCCTCTCATCTTTTCCAATTGTCTTTATTAGTTTCCTTGTTTACTACAAAATTGTGAACAGAATTCATTCAAACCCCCACCATTTGATCATTTTAAAATAGTGTATTGCTCTGCCTCTAACCAGTGTGCTTTCTTTTCTTTTTCTTTCATTGTTTGGTTTTAACAACTTGAGTTTGTTAAAGAGATGCAACAAACTATTCCAATTGAGAAGTGTTGATATTGGAGATTGTCTCACTTCTTTCCCCCCAAAAAAGAAAAAAGAACTTTTGTTCTTTTTTATCACTCTTTCTCTTATTTTTATGAGCTGTTAAAGGCATTGGGGCAAGAACTTGACCACTTTAGTTCATAAGCTTGACAGATTACATAAAATTGTTTCTCTTTGCAACAAAACCATTGCAGCATGATACTCTCTGTCTTTATGACATGGGCTGATGATTTTCCTTTTCTTTTCTTTTCTTTTTTTTTTTTTTTTTTTTTTTGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCACAAAATGTGAATCTCAGGAGAGTGGAGAATTCTAAACAACCACCAGTTCGAGCCTAAGCAAATCAAGGAGATAGATCAGCTGCAGAAAAGGCTCAGAAATGAACAGTTAATTTTTTTTTACCTGTAAGTAAATGGTTTTTATTTTTATTTTTATGCTCTCCATCAAGGATAGATCCTGCATTGAGACTTTTGTCCCCTAAAGCACTGACATGGCAACTGTTCTTAGTGGGTTTTGGGGGGTAAAGAGATTTTGGATAACTTTCCCAGAAAAATTTTCAGTTGTTAGTAGTCATCATTTATTCGATCTAAGGTGCAAAGTTTTTGTTTTTCTCGGAATTTGAAGTTATTATATTTTGTTTCTTGTCGTTCACATGTATATGTGTAACTTGAATTTTCCATTAATATCTTTGTTGTTTTTTTCCCATGCTTTTAGGCGACACATTCTTAAAAAGTGGAAAATGATTGATTCTTGTAATTGGTGATTTAAATAGTAAAAAGTGGCCTATAAACCTGCTCCA

mRNA sequence

ATGGAGTCGGTGGATTGGAAAGCCCTAATATTCACATCCATGAACGTGGGAGAGAGTCCTAAACATGCAAGGACCTCGTATTCTCACATGAATGCAGAATCAATTTACAGTAATCAGAATTCTTGTAACCAGACATGCGAAGCTGCCTGCCTCATGAAATTACAAAGAAAATGGTTCTTATGGGCATCAATCTACTTTGACACTCAAAACCAACTGCCTCAATTTTCTTCAGGAGGGATTGATAATGCTAGCCCAGCATCCAGAAGAGCGACAGACATGCAGTGGAAGGTGATTGGAAGCGAGAAGCATGTCTTTCATTGGCATTCTGATTTCTATTCTGTCAGCAAGAGAAGAGTACCAAATGGACCTGATCCAATCCATAACAGGAGAGTGGAGAATTCTAAACAACCACCAGTTCGAGCCTAAGCAAATCAAGGAGATAGATCAGCTGCAGAAAAGGCTCAGAAATGAACAGTTAATTTTTTTTTACCTGTAAGTAAATGGTTTTTATTTTTATTTTTATGCTCTCCATCAAGGATAGATCCTGCATTGAGACTTTTGTCCCCTAAAGCACTGACATGGCAACTGTTCTTAGTGGGTTTTGGGGGGTAAAGAGATTTTGGATAACTTTCCCAGAAAAATTTTCAGTTGTTAGTAGTCATCATTTATTCGATCTAAGGTGCAAAGTTTTTGTTTTTCTCGGAATTTGAAGTTATTATATTTTGTTTCTTGTCGTTCACATGTATATGTGTAACTTGAATTTTCCATTAATATCTTTGTTGTTTTTTTCCCATGCTTTTAGGCGACACATTCTTAAAAAGTGGAAAATGATTGATTCTTGTAATTGGTGATTTAAATAGTAAAAAGTGGCCTATAAACCTGCTCCA

Coding sequence (CDS)

ATGGAGTCGGTGGATTGGAAAGCCCTAATATTCACATCCATGAACGTGGGAGAGAGTCCTAAACATGCAAGGACCTCGTATTCTCACATGAATGCAGAATCAATTTACAGTAATCAGAATTCTTGTAACCAGACATGCGAAGCTGCCTGCCTCATGAAATTACAAAGAAAATGGTTCTTATGGGCATCAATCTACTTTGACACTCAAAACCAACTGCCTCAATTTTCTTCAGGAGGGATTGATAATGCTAGCCCAGCATCCAGAAGAGCGACAGACATGCAGTGGAAGGTGATTGGAAGCGAGAAGCATGTCTTTCATTGGCATTCTGATTTCTATTCTGTCAGCAAGAGAAGAGTACCAAATGGACCTGATCCAATCCATAACAGGAGAGTGGAGAATTCTAAACAACCACCAGTTCGAGCCTAA

Protein sequence

MESVDWKALIFTSMNVGESPKHARTSYSHMNAESIYSNQNSCNQTCEAACLMKLQRKWFLWASIYFDTQNQLPQFSSGGIDNASPASRRATDMQWKVIGSEKHVFHWHSDFYSVSKRRVPNGPDPIHNRRVENSKQPPVRA
Homology
[Close]
BLAST of CmaCh04G027420 vs. TrEMBL
Match: A0A0A0KS32_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633230 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 5.5e-25
Identity = 55/63 (87.30%), Postives = 58/63 (92.06%), Query Frame = 1 

Query: 78  GGIDNASPASRRATDMQWKVIGSEKHVFHWHSDFYSVSKRRVPNGPDPIHNRRVENSKQP 137
           G IDNAS ASRR TDMQWK+IG EKH FHWHSDFYSVSKRRVPNGPDPIHNRRVENS+QP
Sbjct: 27  GVIDNASLASRRETDMQWKLIGREKHFFHWHSDFYSVSKRRVPNGPDPIHNRRVENSRQP 86

Query: 138 PVR 141
           P+R
Sbjct: 87  PIR 89

[Close]
BLAST of CmaCh04G027420 vs. TrEMBL
Match: M5WZX3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019332mg PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.2e-09
Identity = 30/44 (68.18%), Postives = 33/44 (75.00%), Query Frame = 1 

Query: 95  WKVIGSEKHVFHWHSDFYSVSKRRVPNGPDPIHNRRVENSKQPP 139
           WK+IG EKH   W S F  VSKRRVPNGPDPIHNRR   ++QPP
Sbjct: 50  WKLIGREKHAVVWDSSFIYVSKRRVPNGPDPIHNRRAVKTRQPP 93

[Close]
BLAST of CmaCh04G027420 vs. TrEMBL
Match: A0A067FYD1_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g034498mg PE=4 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 7.9e-08
Identity = 30/46 (65.22%), Postives = 32/46 (69.57%), Query Frame = 1 

Query: 95  WKVIGSEKHVFHWHSDFYSVSKRRVPNGPDPIHNRRVENSKQPPVR 141
           WK+I  EKH  H   D   VSKRRVPNGPDPIHNRR   S+QPP R
Sbjct: 47  WKLIAREKHHAHQDMDLNYVSKRRVPNGPDPIHNRRASKSRQPPGR 92

[Close]
BLAST of CmaCh04G027420 vs. TrEMBL
Match: A0A067LAE6_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_09685 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 5.1e-07
Identity = 28/47 (59.57%), Postives = 32/47 (68.09%), Query Frame = 1 

Query: 95  WKVIGSEKHVFHWHSDFYSVSKRRVPNGPDPIHNRRVENSKQPPVRA 142
           WK+ G  KH  H   D   VSKRRVPNGPDPIHNRR   ++QPP R+
Sbjct: 50  WKLTGRGKHHIHQDLDLNYVSKRRVPNGPDPIHNRRASEARQPPGRS 96

[Close]
BLAST of CmaCh04G027420 vs. TrEMBL
Match: B9HU81_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s04900g PE=4 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 8.7e-07
Identity = 32/51 (62.75%), Postives = 37/51 (72.55%), Query Frame = 1 

Query: 92  DMQWKVIGSEKHVFHWHSDF-YSVSKRRVPNGPDPIHNRRVENSKQPPVRA 142
           D +  VIG EK V++   D  Y +SKRRVPNGPDPIHNRR  NSK+PP RA
Sbjct: 59  DHEKDVIGREKLVYNSELDLNYMMSKRRVPNGPDPIHNRRAGNSKRPPGRA 109

[Close]
BLAST of CmaCh04G027420 vs. TAIR10
Match: AT3G28455.1 (AT3G28455.1 CLAVATA3/ESR-RELATED 25)

HSP 1 Score: 49.3 bits (116), Expect = 2.3e-06
Identity = 24/54 (44.44%), Postives = 35/54 (64.81%), Query Frame = 1 

Query: 85  PASRRATDMQWKVIGSEKHVFHWHSDFYSVSKRRVPNGPDPIHNRRVENSKQPP 139
           P+S     +++   G + ++FH       VSKR+VPNGPDPIHNR+ E S++PP
Sbjct: 35  PSSENVKTLRFS--GKDVNLFH-------VSKRKVPNGPDPIHNRKAETSRRPP 79

[Close]
BLAST of CmaCh04G027420 vs. NCBI nr
Match: gi|659117629|ref|XP_008458704.1| (PREDICTED: CLAVATA3/ESR (CLE)-related protein 25 [Cucumis melo])

HSP 1 Score: 122.5 bits (306), Expect = 6.0e-25
Identity = 55/63 (87.30%), Postives = 58/63 (92.06%), Query Frame = 1 

Query: 78  GGIDNASPASRRATDMQWKVIGSEKHVFHWHSDFYSVSKRRVPNGPDPIHNRRVENSKQP 137
           G IDNAS ASRR TDMQWK+IG EKH FHWHSDFYSVSKRRVPNGPDPIHNRRVENS+QP
Sbjct: 27  GVIDNASLASRRETDMQWKLIGKEKHFFHWHSDFYSVSKRRVPNGPDPIHNRRVENSRQP 86

Query: 138 PVR 141
           P+R
Sbjct: 87  PIR 89

[Close]
BLAST of CmaCh04G027420 vs. NCBI nr
Match: gi|700197248|gb|KGN52425.1| (hypothetical protein Csa_5G633230 [Cucumis sativus])

HSP 1 Score: 122.1 bits (305), Expect = 7.8e-25
Identity = 55/63 (87.30%), Postives = 58/63 (92.06%), Query Frame = 1 

Query: 78  GGIDNASPASRRATDMQWKVIGSEKHVFHWHSDFYSVSKRRVPNGPDPIHNRRVENSKQP 137
           G IDNAS ASRR TDMQWK+IG EKH FHWHSDFYSVSKRRVPNGPDPIHNRRVENS+QP
Sbjct: 27  GVIDNASLASRRETDMQWKLIGREKHFFHWHSDFYSVSKRRVPNGPDPIHNRRVENSRQP 86

Query: 138 PVR 141
           P+R
Sbjct: 87  PIR 89

[Close]
BLAST of CmaCh04G027420 vs. NCBI nr
Match: gi|657974945|ref|XP_008379312.1| (PREDICTED: CLAVATA3/ESR (CLE)-related protein 25 [Malus domestica])

HSP 1 Score: 74.7 bits (182), Expect = 1.4e-10
Identity = 39/84 (46.43%), Postives = 45/84 (53.57%), Query Frame = 1 

Query: 58  WFLWASIYFDTQNQLPQFSSGGIDNASPASRRATDMQWKVIGSEKHVFHWHSDFYSVSKR 117
           WF  A I  +   + P         A   S       WK+IG EKH   W S F  VSKR
Sbjct: 22  WFFLAGILVNRSAEKP---------AVTVSPTEIFKHWKLIGREKHAVVWDSTFIYVSKR 81

Query: 118 RVPNGPDPIHNRRVENSKQPPVRA 142
           RVPNGPDPIHNRR   ++QPP+RA
Sbjct: 82  RVPNGPDPIHNRRAVKTRQPPMRA 96

[Close]
BLAST of CmaCh04G027420 vs. NCBI nr
Match: gi|645249317|ref|XP_008230697.1| (PREDICTED: CLAVATA3/ESR (CLE)-related protein 25 [Prunus mume])

HSP 1 Score: 72.8 bits (177), Expect = 5.4e-10
Identity = 32/47 (68.09%), Postives = 35/47 (74.47%), Query Frame = 1 

Query: 95  WKVIGSEKHVFHWHSDFYSVSKRRVPNGPDPIHNRRVENSKQPPVRA 142
           WK+IG EKH   W S F  VSKRRVPNGPDPIHNRR   ++QPP RA
Sbjct: 50  WKLIGREKHAVVWDSSFIYVSKRRVPNGPDPIHNRRAVKTRQPPTRA 96

[Close]
BLAST of CmaCh04G027420 vs. NCBI nr
Match: gi|658012485|ref|XP_008341510.1| (PREDICTED: CLAVATA3/ESR (CLE)-related protein 25-like [Malus domestica])

HSP 1 Score: 72.4 bits (176), Expect = 7.1e-10
Identity = 32/47 (68.09%), Postives = 35/47 (74.47%), Query Frame = 1 

Query: 95  WKVIGSEKHVFHWHSDFYSVSKRRVPNGPDPIHNRRVENSKQPPVRA 142
           WK+IG EKH   W S F  VSKRRVPNGPDPIHNRR   ++QPP RA
Sbjct: 51  WKLIGREKHTVVWDSTFIYVSKRRVPNGPDPIHNRRAVKTRQPPTRA 97

The following BLAST results are available for this feature:
BLAST of CmaCh04G027420 vs. Swiss-Prot
Match NameE-valueIdentityDescription
BLAST of CmaCh04G027420 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KS32_CUCSA5.5e-2587.30Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633230 PE=4 SV=1[more]
M5WZX3_PRUPE4.2e-0968.18Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019332mg PE=4 SV=1[more]
A0A067FYD1_CITSI7.9e-0865.22Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g034498mg PE=4 SV=1[more]
A0A067LAE6_JATCU5.1e-0759.57Uncharacterized protein OS=Jatropha curcas GN=JCGZ_09685 PE=4 SV=1[more]
B9HU81_POPTR8.7e-0762.75Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s04900g PE=4 SV=1[more]
BLAST of CmaCh04G027420 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G28455.12.3e-0644.44 CLAVATA3/ESR-RELATED 25[more]
BLAST of CmaCh04G027420 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659117629|ref|XP_008458704.1|6.0e-2587.30PREDICTED: CLAVATA3/ESR (CLE)-related protein 25 [Cucumis melo][more]
gi|700197248|gb|KGN52425.1|7.8e-2587.30hypothetical protein Csa_5G633230 [Cucumis sativus][more]
gi|657974945|ref|XP_008379312.1|1.4e-1046.43PREDICTED: CLAVATA3/ESR (CLE)-related protein 25 [Malus domestica][more]
gi|645249317|ref|XP_008230697.1|5.4e-1068.09PREDICTED: CLAVATA3/ESR (CLE)-related protein 25 [Prunus mume][more]
gi|658012485|ref|XP_008341510.1|7.1e-1068.09PREDICTED: CLAVATA3/ESR (CLE)-related protein 25-like [Malus domestica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0045168 cell-cell signaling involved in cell fate commitment
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G027420.1CmaCh04G027420.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR34277FAMILY NOT NAMEDcoord: 86..141
score: 1.6
NoneNo IPR availablePANTHERPTHR34277:SF2CLAVATA3/ESR (CLE)-RELATED PROTEIN 26coord: 86..141
score: 1.6

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh04G027420Cucsa.158640Cucumber (Gy14) v1cgycmaB0441
CmaCh04G027420Cla010358Watermelon (97103) v1cmawmB652
CmaCh04G027420Csa5G633230Cucumber (Chinese Long) v2cmacuB723
CmaCh04G027420ClCG09G018520Watermelon (Charleston Gray)cmawcgB608
CmaCh04G027420CSPI05G28630Wild cucumber (PI 183967)cmacpiB730
CmaCh04G027420CmoCh04G028720Cucurbita moschata (Rifu)cmacmoB729
CmaCh04G027420CmoCh15G003100Cucurbita moschata (Rifu)cmacmoB692
CmaCh04G027420Lsi02G015150Bottle gourd (USVL1VR-Ls)cmalsiB651
CmaCh04G027420Cp4.1LG13g08540Cucurbita pepo (Zucchini)cmacpeB705
CmaCh04G027420Cp4.1LG01g23020Cucurbita pepo (Zucchini)cmacpeB721
CmaCh04G027420CsaV3_5G037710Cucumber (Chinese Long) v3cmacucB0860
CmaCh04G027420Cla97C09G179940Watermelon (97103) v2cmawmbB797
CmaCh04G027420Bhi06G000381Wax gourdcmawgoB0839
CmaCh04G027420CsGy5G027940Cucumber (Gy14) v2cgybcmaB647
CmaCh04G027420Carg02269Silver-seed gourdcarcmaB0374
CmaCh04G027420Carg04406Silver-seed gourdcarcmaB0967
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh04G027420CmaCh15G002960Cucurbita maxima (Rimu)cmacmaB324
The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh04G027420Cucumber (Chinese Long) v3cmacucB0818
CmaCh04G027420Cucumber (Chinese Long) v3cmacucB0839
CmaCh04G027420Watermelon (97103) v2cmawmbB714
CmaCh04G027420Cucumber (Gy14) v1cgycmaB0939
CmaCh04G027420Wild cucumber (PI 183967)cmacpiB711
CmaCh04G027420Cucumber (Chinese Long) v2cmacuB704
CmaCh04G027420Melon (DHL92) v3.5.1cmameB634
CmaCh04G027420Melon (DHL92) v3.5.1cmameB685
CmaCh04G027420Bottle gourd (USVL1VR-Ls)cmalsiB655
CmaCh04G027420Bottle gourd (USVL1VR-Ls)cmalsiB658
CmaCh04G027420Cucumber (Gy14) v2cgybcmaB132
CmaCh04G027420Cucumber (Gy14) v2cgybcmaB418
CmaCh04G027420Melon (DHL92) v3.6.1cmamedB720
CmaCh04G027420Melon (DHL92) v3.6.1cmamedB750
CmaCh04G027420Melon (DHL92) v3.6.1cmamedB769