Carg21988 (gene) Silver-seed gourd

Overview
NameCarg21988
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionGibberellin 2-beta-dioxygenase 8
LocationCucurbita_argyrosperma_scaffold_186 : 121283 .. 122946 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTAAGGAAGATACCACAATGATGAACTCCAATCCACCACTCTTCCAACAATATGAGTTGCTTTTGCAATCCACAGCTCATTTACAGCCACTTCACAATGGCTATCAACCAACTAGCTCTGTCCTGGAGGAATGTCAGCTCCCTCTTGTCGATCTTAAAGGTTTGGAAAGTGGCAAAGAAAAAGAGAGGATGGCTTGCAGTAGAGAGATTTTTGAAGCATCTGCAGAATGGGGATTTTTCCAAGTGATTAACCATGGGATACGCTCAGAGCTGCTGAGCAGAATGAGCAAAGAACAAATGAAGTTGTTTGCGGTGCCCTTTGAGAAAAAATCTACAAGTGGGATTTTGGATAATTCTTACAGATGGGGAGCAGCCACTGCAACCCAACCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAACTGACTGCTATGGCGACTTCATTCACTTAAGGTATAGATTTTATTACCCTTCTTCCAACTAGAAACTTCTCTTGAGCATTGTGTCAAATTTATTTAGTTCATATGCTCAAAACACAGTCTATTTAGCCTTTGAACTTGCACACACACAAAAAAAAGAAAGAAGAAAAAACCCCATTTCATTCTAGGTTATGTTTTTCTTTGTTAACATCAGAATGCTTAATATGACAGGCTGTTCGCCTGTCCAGTTGTTTGCTATAATATCGCACCAACTCAGCTAAGAGTCATATAAAAACATGTCACGATAGCATTCCTTGATAGTTTCAATGACAATCACTAAATAAAAGCATAATTCAAAATTTGACAATATAATAAAACAAATCCAAAACCCAACGTCTAAGGACTATACTTGTATCTTTAACATTGAAATATTCATGCACTTGAAGATTTTAGGTCTTCTAGACATTTTCACAAACAGTTCGACAGCAAAAGTAATGCACTTCGATATCACAATCAACCAATGTACTAGATTACAAAAACAAAGAGAGTGCATTGCTCACTGACCTTACATCCTCGAACGCCAGGGAAGTGATGGAGGAACTCGCAACCGCCATGTCGAAGCTCGCAAGATCTCTAGCAGGAGTTTTAGTGGAGAGTTTGGGGCACCGGAAGGAAGTATTTGAAGATATGTGCGATGAAAGCACATGCTTCCTCCGGTTGAATCACTACCCAATCTGTCCGTTTCCCGGCGAAGTTACCGGCCTGGTGCCCCACACCGATAGCGATTATCTGACCATACTTCATCAAGATTCCGGCGGCGGGCTTGAGCTGATGAAAGGATCGCAATGGGTGGCCGTGAAACCCATTCCGGAAGCCCTTGTCGTTAACATTGGAGATCTTCTACAGGTACAAACTGAAACTCTTCCGAAGTATAATGGTTTGGTAGAGCAAGTAGAAAGTGGGTATAAAAATATAGTTTGAAATGCAGGCTTGGAGCAACGATACGTACAAGAGTGTGAAGCATAGGGTGGTGACTAACTCGGTGCGGGAGAGATATTCGACAGCATACTTCCTCTGCCCATCTAACCATTCTGGAATCGGGAGTTGCCGGCAGCCTTCACCATACAGACACTTCACTTTTGGGGAGTACAGGAAGCAAGTGAGAGATGATGTGGAAAAAACTGGGTACAAAGTAGGGCTTTCCAGATTTCGGCTGTAA

mRNA sequence

ATGTTTAAGGAAGATACCACAATGATGAACTCCAATCCACCACTCTTCCAACAATATGAGTTGCTTTTGCAATCCACAGCTCATTTACAGCCACTTCACAATGGCTATCAACCAACTAGCTCTGTCCTGGAGGAATGTCAGCTCCCTCTTGTCGATCTTAAAGGTTTGGAAAGTGGCAAAGAAAAAGAGAGGATGGCTTGCAGTAGAGAGATTTTTGAAGCATCTGCAGAATGGGGATTTTTCCAAGTGATTAACCATGGGATACGCTCAGAGCTGCTGAGCAGAATGAGCAAAGAACAAATGAAGTTGTTTGCGGTGCCCTTTGAGAAAAAATCTACAAGTGGGATTTTGGATAATTCTTACAGATGGGGAGCAGCCACTGCAACCCAACCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAACTGACTGCTATGGCGACTTCATTCACTTAAGGGAAGTGATGGAGGAACTCGCAACCGCCATGTCGAAGCTCGCAAGATCTCTAGCAGGAGTTTTAGTGGAGAGTTTGGGGCACCGGAAGGAAGTATTTGAAGATATGTGCGATGAAAGCACATGCTTCCTCCGGTTGAATCACTACCCAATCTGTCCGTTTCCCGGCGAAGTTACCGGCCTGGTGCCCCACACCGATAGCGATTATCTGACCATACTTCATCAAGATTCCGGCGGCGGGCTTGAGCTGATGAAAGGATCGCAATGGGTGGCCGTGAAACCCATTCCGGAAGCCCTTGTCGTTAACATTGGAGATCTTCTACAGGCTTGGAGCAACGATACGTACAAGAGTGTGAAGCATAGGGTGGTGACTAACTCGGTGCGGGAGAGATATTCGACAGCATACTTCCTCTGCCCATCTAACCATTCTGGAATCGGGAGTTGCCGGCAGCCTTCACCATACAGACACTTCACTTTTGGGGAGTACAGGAAGCAAGTGAGAGATGATGTGGAAAAAACTGGGTACAAAGTAGGGCTTTCCAGATTTCGGCTGTAA

Coding sequence (CDS)

ATGTTTAAGGAAGATACCACAATGATGAACTCCAATCCACCACTCTTCCAACAATATGAGTTGCTTTTGCAATCCACAGCTCATTTACAGCCACTTCACAATGGCTATCAACCAACTAGCTCTGTCCTGGAGGAATGTCAGCTCCCTCTTGTCGATCTTAAAGGTTTGGAAAGTGGCAAAGAAAAAGAGAGGATGGCTTGCAGTAGAGAGATTTTTGAAGCATCTGCAGAATGGGGATTTTTCCAAGTGATTAACCATGGGATACGCTCAGAGCTGCTGAGCAGAATGAGCAAAGAACAAATGAAGTTGTTTGCGGTGCCCTTTGAGAAAAAATCTACAAGTGGGATTTTGGATAATTCTTACAGATGGGGAGCAGCCACTGCAACCCAACCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAACTGACTGCTATGGCGACTTCATTCACTTAAGGGAAGTGATGGAGGAACTCGCAACCGCCATGTCGAAGCTCGCAAGATCTCTAGCAGGAGTTTTAGTGGAGAGTTTGGGGCACCGGAAGGAAGTATTTGAAGATATGTGCGATGAAAGCACATGCTTCCTCCGGTTGAATCACTACCCAATCTGTCCGTTTCCCGGCGAAGTTACCGGCCTGGTGCCCCACACCGATAGCGATTATCTGACCATACTTCATCAAGATTCCGGCGGCGGGCTTGAGCTGATGAAAGGATCGCAATGGGTGGCCGTGAAACCCATTCCGGAAGCCCTTGTCGTTAACATTGGAGATCTTCTACAGGCTTGGAGCAACGATACGTACAAGAGTGTGAAGCATAGGGTGGTGACTAACTCGGTGCGGGAGAGATATTCGACAGCATACTTCCTCTGCCCATCTAACCATTCTGGAATCGGGAGTTGCCGGCAGCCTTCACCATACAGACACTTCACTTTTGGGGAGTACAGGAAGCAAGTGAGAGATGATGTGGAAAAAACTGGGTACAAAGTAGGGCTTTCCAGATTTCGGCTGTAA

Protein sequence

MFKEDTTMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAATATQPNQFSWSEAFHIPLTKVSETDCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLELMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL
Homology
[Close]
BLAST of Carg21988 vs. NCBI nr
Match: XP_022942818.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita moschata])

HSP 1 Score: 704.1 bits (1816), Expect = 2.3e-199
Identity = 340/343 (99.13%), Postives = 341/343 (99.42%), Query Frame = 0 

Query: 1   MFKEDTTMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGK 60
           MFKEDTTMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGK
Sbjct: 1   MFKEDTTMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGK 60

Query: 61  EKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNS 120
           EKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNS
Sbjct: 61  EKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNS 120

Query: 121 YRWGAATATQPNQFSWSEAFHIPLTKVSETDCYGDFIHLREVMEELATAMSKLARSLAGV 180
           YRWGAATATQPNQFSWSEAFHIPLTKVSE DCYGDFIHLREVMEELATAMSKLARSLAGV
Sbjct: 121 YRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGV 180

Query: 181 LVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGL 240
           LVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGL
Sbjct: 181 LVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGL 240

Query: 241 ELMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSN 300
           +LMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSN
Sbjct: 241 QLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSN 300

Query: 301 HSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 344
            SGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL
Sbjct: 301 QSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 343

[Close]
BLAST of Carg21988 vs. NCBI nr
Match: XP_023514448.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 691.4 bits (1783), Expect = 1.5e-195
Identity = 333/336 (99.11%), Postives = 334/336 (99.40%), Query Frame = 0 

Query: 8   MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMAC 67
           MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMAC
Sbjct: 1   MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMAC 60

Query: 68  SREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAAT 127
           SREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFE KSTSGILDNSYRWGAAT
Sbjct: 61  SREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFENKSTSGILDNSYRWGAAT 120

Query: 128 ATQPNQFSWSEAFHIPLTKVSETDCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGH 187
           ATQPNQFSWSEAFHIPLTKVSE DCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGH
Sbjct: 121 ATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGH 180

Query: 188 RKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLELMKGSQ 247
           RKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQD+GGGLELMKGSQ
Sbjct: 181 RKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDAGGGLELMKGSQ 240

Query: 248 WVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIGSC 307
           WVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIGSC
Sbjct: 241 WVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIGSC 300

Query: 308 RQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 344
           RQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL
Sbjct: 301 RQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 336

[Close]
BLAST of Carg21988 vs. NCBI nr
Match: XP_022985325.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita maxima])

HSP 1 Score: 682.6 bits (1760), Expect = 7.1e-193
Identity = 329/343 (95.92%), Postives = 335/343 (97.67%), Query Frame = 0 

Query: 1   MFKEDTTMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGK 60
           MFKEDTT+MNSNPPLF QY LLLQSTAHLQPL +GYQPTSSVLEECQLPLVDLKGLESGK
Sbjct: 1   MFKEDTTVMNSNPPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESGK 60

Query: 61  EKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNS 120
           EKERMACS EIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNS
Sbjct: 61  EKERMACSSEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNS 120

Query: 121 YRWGAATATQPNQFSWSEAFHIPLTKVSETDCYGDFIHLREVMEELATAMSKLARSLAGV 180
           YRWGAATATQPNQFSWSEAFHIPLTKVSE DCYG+FIHLREVMEELATAMSKLARSLAGV
Sbjct: 121 YRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGNFIHLREVMEELATAMSKLARSLAGV 180

Query: 181 LVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGL 240
           LVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPG+VTGLVPHTDSDYLTILHQDSGGGL
Sbjct: 181 LVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGGL 240

Query: 241 ELMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSN 300
           +LMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVV NS RERYSTAYFLCPS+
Sbjct: 241 QLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVANSARERYSTAYFLCPSD 300

Query: 301 HSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 344
           HSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYK GLSRFRL
Sbjct: 301 HSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKEGLSRFRL 343

[Close]
BLAST of Carg21988 vs. NCBI nr
Match: XP_022942053.1 (gibberellin 2-beta-dioxygenase 8-like, partial [Cucurbita moschata])

HSP 1 Score: 604.0 bits (1556), Expect = 3.2e-169
Identity = 289/340 (85.00%), Postives = 310/340 (91.18%), Query Frame = 0 

Query: 4   EDTTMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKE 63
           E+TTM +SNPPLF QYELLLQS A LQ L+NG Q  +SV+EECQLPL+DL GLES  EKE
Sbjct: 37  ENTTMTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKE 96

Query: 64  RMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRW 123
           R ACSREIF ASAEWGFFQVINHGIR+ELLSRMSKEQMKLF VPFEKKSTSGILD+SYRW
Sbjct: 97  RRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRW 156

Query: 124 GAATATQPNQFSWSEAFHIPLTKVSETDCYGDFIHLREVMEELATAMSKLARSLAGVLVE 183
           G  TAT PNQFSWSEAFHIPLTKVSE  CYG+FI+LREVMEELATAMSKLARSLAGVLVE
Sbjct: 157 GTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVE 216

Query: 184 SLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLELM 243
            LGHRKE+F+D+CDESTCFLRLNHYPICPFPG+V+GLVPHTDSD+LTILHQD  GGL+LM
Sbjct: 217 CLGHRKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLM 276

Query: 244 KGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSG 303
           KGSQWVAVKP PEALVVNIGDLLQAWSNDTYKSVKH VV NS+RERYS AYFLCPS HS 
Sbjct: 277 KGSQWVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHMVVANSMRERYSIAYFLCPSYHSP 336

Query: 304 IGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 344
           IGSC+QPSPYRHFTF EYRKQVRDDVEK GYK+GLS+FRL
Sbjct: 337 IGSCKQPSPYRHFTFAEYRKQVRDDVEKIGYKIGLSKFRL 376

[Close]
BLAST of Carg21988 vs. NCBI nr
Match: XP_004142323.2 (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus] >KGN49660.1 hypothetical protein Csa_5G052540 [Cucumis sativus])

HSP 1 Score: 602.1 bits (1551), Expect = 1.2e-168
Identity = 285/344 (82.85%), Postives = 313/344 (90.99%), Query Frame = 0 

Query: 1   MFKEDTT-MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESG 60
           MFKE+TT MM+SNPPLF QYE LLQSTAHLQP +NG  PT+SV+EECQLPL+DLKGL+S 
Sbjct: 1   MFKENTTMMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSS 60

Query: 61  KEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDN 120
            EKER+AC REIFEAS EWGFFQVINHGI +ELL+RM+KEQ+KLF VPFEKK TSGILDN
Sbjct: 61  DEKERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDN 120

Query: 121 SYRWGAATATQPNQFSWSEAFHIPLTKVSETDCYGDFIHLREVMEELATAMSKLARSLAG 180
           SYRWG  TAT PNQFSWSEAFHIPLTKVSE  CYGDFI+LREVMEE+A+AMSK+AR LAG
Sbjct: 121 SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAG 180

Query: 181 VLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGG 240
           VLVES+G RKE+ ED+CDESTCFLRLNHYPICPF GEV+GLVPHTDSD+LTILHQDSGGG
Sbjct: 181 VLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGG 240

Query: 241 LELMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPS 300
           L++MKGSQW+AVKP P+ALVVNIGDLLQAWSNDTYKS KHRVV N +RERYS AYFLCPS
Sbjct: 241 LQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHRVVANPMRERYSIAYFLCPS 300

Query: 301 NHSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 344
            HS IGSCR+PSPYRHFTF EYRKQVRDDVEKTGYK+GLS FRL
Sbjct: 301 YHSQIGSCREPSPYRHFTFAEYRKQVRDDVEKTGYKIGLSNFRL 344

[Close]
BLAST of Carg21988 vs. TAIR10
Match: AT4G21200.1 (gibberellin 2-oxidase 8)

HSP 1 Score: 332.0 bits (850), Expect = 4.3e-91
Identity = 159/303 (52.48%), Postives = 211/303 (69.64%), Query Frame = 0 

Query: 40  SSVLEECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKE 99
           ++V+EE +LP++D+  L  G E+ER  C   I  AS EWGFFQVINHGI  ++L +M +E
Sbjct: 34  TAVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQE 93

Query: 100 QMKLFAVPFEKKSTSGILD-NSYRWGAATATQPNQFSWSEAFHIPLTKVSETDCYGDFIH 159
           Q+++F  PF+KKS S      SYRWG  +AT   Q SWSEAFH+P+T +S+     DF  
Sbjct: 94  QIRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFTT 153

Query: 160 LREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVT 219
           L   ME+ A+    LA  LA VL E  G     F++ C  +TC+LR+N YP CP P EV 
Sbjct: 154 LSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVY 213

Query: 220 GLVPHTDSDYLTILHQDSGGGLELMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVK 279
           GL+PHTDSD+LTIL+QD  GGL+L+K ++W+AVKP P+AL++NIGDL QAWSN  YKSV+
Sbjct: 214 GLMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVE 273

Query: 280 HRVVTNSVRERYSTAYFLCPSNHSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGL 339
           HRV+TN   ER+STAYF+CPS  + I        YR+F+F E+R+QV++DV+K G+KVGL
Sbjct: 274 HRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGL 333

Query: 340 SRF 342
            RF
Sbjct: 334 PRF 333

[Close]
BLAST of Carg21988 vs. TAIR10
Match: AT1G50960.1 (gibberellin 2-oxidase 7)

HSP 1 Score: 261.2 bits (666), Expect = 9.3e-70
Identity = 140/336 (41.67%), Postives = 200/336 (59.52%), Query Frame = 0 

Query: 9   MNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMACS 68
           M S PP    +  +  S+       +    ++      +LP++DL  L SG+E +R  C 
Sbjct: 1   MASQPPFKTNFCSIFGSSFPNSTSESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCV 60

Query: 69  REIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFE---KKSTSGILDNSYRWGA 128
           +++  A+ EWGFFQ++NHGI  ++   M  E+ KLF  PF    ++  S +  NSYRWG 
Sbjct: 61  KQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQPFSVKVRERFSDLSKNSYRWGN 120

Query: 129 ATATQPNQFSWSEAFHIPLTKVSETDCYGDFIHLREVMEELATAMSKLARSLAGVLVESL 188
            +AT P Q+S SEAFHI L++VS      D  +LR ++E     ++++A+ +  +L + +
Sbjct: 121 PSATSPAQYSVSEAFHIILSEVSRIS--DDRNNLRTIVETYVQEIARVAQMICEILGKQV 180

Query: 189 GHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLELMKG 248
               E FE++ +    FLRLN Y    F  EV GLVPHTD+ +LTIL QD  GGLEL   
Sbjct: 181 NVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENN 240

Query: 249 SQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIG 308
            QW++VKP  EAL VNIGD+ QA SN  Y+SV+HRV++ +  ER S A+F+CP   + I 
Sbjct: 241 GQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPANIERMSIAFFVCPYLETEID 300

Query: 309 SCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRF 342
               P  YR F+F EY++Q   DV++TG KVGLSRF
Sbjct: 301 CFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRF 334

[Close]
BLAST of Carg21988 vs. TAIR10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 171.8 bits (434), Expect = 7.4e-43
Identity = 107/313 (34.19%), Postives = 157/313 (50.16%), Query Frame = 0 

Query: 47  QLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAV 106
           ++P++DL  L+  ++ + +    EI +A  +WGFFQV+NHG+ S+   R+ K     F +
Sbjct: 27  EIPVIDLSRLDDPEDVQNVI--SEIGDACEKWGFFQVLNHGVPSDARQRVEKTVKMFFDL 86

Query: 107 PFEKK---STSGILDNSYRWGAATATQPNQFSWSEAFHI----PLTKVSETDCYGDFIHL 166
           P E+K       +    Y  G  T    N   W E F I    P+   S TD   + + L
Sbjct: 87  PMEEKIKVKRDDVNPVGYHDGEHT---KNVKDWKEVFDIYFKDPMVIPSTTDPEDEGLRL 146

Query: 167 ------------REVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNH 226
                       RE  E  A    KLA  L  ++  SLG  KE F D   E   F R+N 
Sbjct: 147 VYNKWPQSPSDFREACEVYARHAEKLAFKLLELISLSLGLPKERFHDYFKEQMSFFRINR 206

Query: 227 YPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLELMKGSQ--WVAVKPIPEALVVNIGDL 286
           YP CP P    G+  H D+D +++L QD  GGL++ + S   W  ++P+P ALV+NIG+ 
Sbjct: 207 YPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRRSDGVWFPIRPVPNALVINIGNC 266

Query: 287 LQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNH------SGIGSCRQPSPYRHFTFG 332
           ++ W+ND Y S +HRVV N+ RERYS  +FL PS+         + S   P  Y+ + +G
Sbjct: 267 MEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHDVEVKPLEELVSPENPPKYKGYKWG 326

[Close]
BLAST of Carg21988 vs. TAIR10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 169.9 bits (429), Expect = 2.8e-42
Identity = 102/299 (34.11%), Postives = 152/299 (50.84%), Query Frame = 0 

Query: 47  QLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAV 106
           ++P +DL  LE     ++ A ++EI EA   WGFFQVINHG+ S L  R+ K   + F +
Sbjct: 31  EIPTIDLSSLED-THHDKTAIAKEIAEACKRWGFFQVINHGLPSALRHRVEKTAAEFFNL 90

Query: 107 PFEKK--------STSGILDNSYRWGAATATQPNQFSWSEAFHIPLTKVSETDCYGDFI- 166
             E+K        +  G  D  +        +   F   ++  +P +   E         
Sbjct: 91  TTEEKRKVKRDEVNPMGYHDEEHTKNVRDWKEIFDFFLQDSTIVPASPEPEDTELRKLTN 150

Query: 167 -------HLREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPI 226
                  H REV +E A  + KLA  L  ++  SLG   +      +E T FLR NHYP 
Sbjct: 151 QWPQNPSHFREVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNEQTSFLRFNHYPP 210

Query: 227 CPFPGEVTGLVPHTDSDYLTILHQDSGGGLELMKGS--QWVAVKPIPEALVVNIGDLLQA 286
           CP P    G+  H D   LT+L QDS GGL++ + S  QW+ VKPI +AL++N+G+ +Q 
Sbjct: 211 CPNPELALGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIINMGNCIQV 270

Query: 287 WSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIG------SCRQPSPYRHFTFGEY 322
           W+ND Y S +HRVV N+ +ER+S  +F  PS+ + I       S   P  Y+ + +G++
Sbjct: 271 WTNDEYWSAEHRVVVNTSKERFSIPFFFFPSHEANIEPLEELISEENPPCYKKYNWGKF 328

[Close]
BLAST of Carg21988 vs. TAIR10
Match: AT5G58660.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 169.1 bits (427), Expect = 4.8e-42
Identity = 113/332 (34.04%), Postives = 168/332 (50.60%), Query Frame = 0 

Query: 44  EECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKL 103
           ++  +P++DL+ L+  KE  R AC         EWG F++ NHG+   L SR+ +    L
Sbjct: 30  KDINIPVIDLERLD--KEILREACK--------EWGIFRLENHGVPLALTSRLQEISESL 89

Query: 104 FAVPFEKKS---TSGILDNSYRWGAAT----------ATQPNQFSWSEAFHIPLTKVS-- 163
            ++PFEKK     +     SY WG               Q +  +  E F++PL+ +S  
Sbjct: 90  LSLPFEKKRELFAAVKSPLSYFWGTPALNRSGDALKRGAQASNLTMLEGFNVPLSSLSSL 149

Query: 164 -----ETDCYGD------FIHLREVMEELATAMSKLARSLAGVLVESL-----GHRKEVF 223
                 T C  D          R +MEE    ++++A SL   + ++L     G+R+   
Sbjct: 150 SKLPTSTCCDDDAQEEPKLESFRVLMEEYGKHITRIAVSLFEAIAQTLNLELSGNRR--- 209

Query: 224 EDMCDESTCFLRLNHYPICPFPG--EVTGLVPHTDSDYLTILHQDSGGGLELMKGSQWVA 283
            +   EST  +R+  YP        E  G+  HTDS  ++IL +D  GGLE+MKG +W  
Sbjct: 210 SEYLSESTGLIRVYRYPQSSEEAAREALGMEVHTDSSVISILREDESGGLEIMKGEEWFC 269

Query: 284 VKPIPEALVVNIGDLLQAWSNDTYKSVKHRV-VTNSVRERYSTAYFLCPSNHSGIGSCRQ 342
           VKP+   L+VN+GD++QA S+D YKSV HRV   N   ER+S  YF+ P     I S   
Sbjct: 270 VKPVANTLIVNLGDMMQAISDDEYKSVTHRVKKRNRKTERHSVCYFVFPKRDCVIKS--- 329

[Close]
BLAST of Carg21988 vs. Swiss-Prot
Match: sp|Q7XP65|G2OX6_ORYSJ (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 5.0e-97
Identity = 171/305 (56.07%), Postives = 214/305 (70.16%), Query Frame = 0 

Query: 45  ECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLF 104
           E  LP+VDL+ L  G   ER AC+  +  A++EWGFFQ+ NHG+  EL+  M +EQ +LF
Sbjct: 47  ERDLPMVDLERLTRGGAGERKACAGAMARAASEWGFFQLTNHGVGRELMEEMRREQARLF 106

Query: 105 AVPFEKKSTSGILDNSYRWGAATATQPNQFSWSEAFHIPLTKVSETDC-YGDFIHLREVM 164
            +PFE K  +G+L+ SYRWG  TAT     SWSEAFH+PL  +S  DC +GD   LR VM
Sbjct: 107 RLPFETKEKAGLLNGSYRWGNPTATSLRHLSWSEAFHVPLASISGADCDFGDLTSLRGVM 166

Query: 165 EELATAMSKLARSLAGVLVESLGHR-------KEVFEDMCDESTCFLRLNHYPICPFPGE 224
           +E+A AMS++A ++A  L E L  R          F   CDE+TCFLRLN YP CPF  +
Sbjct: 167 QEVAEAMSRVANTVAAALAEELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPACPFAAD 226

Query: 225 VTGLVPHTDSDYLTILHQDSGGGLELMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKS 284
             GLVPHTDSD+LT+L QD  GGL LMK S+WVAV+P P+ALVVNIGDL QAWSN+ YKS
Sbjct: 227 TFGLVPHTDSDFLTVLCQDQVGGLHLMKDSRWVAVRPRPDALVVNIGDLFQAWSNNRYKS 286

Query: 285 VKHRVVTNSVRERYSTAYFLCPSNHSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKV 342
           V+H+VV N+  +R S AYFLCPS  S +G+C +PSPYR FTFGEYRK+V++DV  TG K+
Sbjct: 287 VEHKVVANAKTDRLSVAYFLCPSYDSLVGTCGEPSPYRAFTFGEYRKKVQEDVRTTGKKI 346

[Close]
BLAST of Carg21988 vs. Swiss-Prot
Match: sp|O49561|G2OX8_ARATH (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 332.0 bits (850), Expect = 7.7e-90
Identity = 159/303 (52.48%), Postives = 211/303 (69.64%), Query Frame = 0 

Query: 40  SSVLEECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKE 99
           ++V+EE +LP++D+  L  G E+ER  C   I  AS EWGFFQVINHGI  ++L +M +E
Sbjct: 34  TAVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQE 93

Query: 100 QMKLFAVPFEKKSTSGILD-NSYRWGAATATQPNQFSWSEAFHIPLTKVSETDCYGDFIH 159
           Q+++F  PF+KKS S      SYRWG  +AT   Q SWSEAFH+P+T +S+     DF  
Sbjct: 94  QIRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFTT 153

Query: 160 LREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVT 219
           L   ME+ A+    LA  LA VL E  G     F++ C  +TC+LR+N YP CP P EV 
Sbjct: 154 LSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVY 213

Query: 220 GLVPHTDSDYLTILHQDSGGGLELMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVK 279
           GL+PHTDSD+LTIL+QD  GGL+L+K ++W+AVKP P+AL++NIGDL QAWSN  YKSV+
Sbjct: 214 GLMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVE 273

Query: 280 HRVVTNSVRERYSTAYFLCPSNHSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGL 339
           HRV+TN   ER+STAYF+CPS  + I        YR+F+F E+R+QV++DV+K G+KVGL
Sbjct: 274 HRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGL 333

Query: 340 SRF 342
            RF
Sbjct: 334 PRF 333

[Close]
BLAST of Carg21988 vs. Swiss-Prot
Match: sp|Q9C6I4|G2OX7_ARATH (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.7e-68
Identity = 140/336 (41.67%), Postives = 200/336 (59.52%), Query Frame = 0 

Query: 9   MNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMACS 68
           M S PP    +  +  S+       +    ++      +LP++DL  L SG+E +R  C 
Sbjct: 1   MASQPPFKTNFCSIFGSSFPNSTSESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCV 60

Query: 69  REIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFE---KKSTSGILDNSYRWGA 128
           +++  A+ EWGFFQ++NHGI  ++   M  E+ KLF  PF    ++  S +  NSYRWG 
Sbjct: 61  KQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQPFSVKVRERFSDLSKNSYRWGN 120

Query: 129 ATATQPNQFSWSEAFHIPLTKVSETDCYGDFIHLREVMEELATAMSKLARSLAGVLVESL 188
            +AT P Q+S SEAFHI L++VS      D  +LR ++E     ++++A+ +  +L + +
Sbjct: 121 PSATSPAQYSVSEAFHIILSEVSRIS--DDRNNLRTIVETYVQEIARVAQMICEILGKQV 180

Query: 189 GHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLELMKG 248
               E FE++ +    FLRLN Y    F  EV GLVPHTD+ +LTIL QD  GGLEL   
Sbjct: 181 NVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENN 240

Query: 249 SQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIG 308
            QW++VKP  EAL VNIGD+ QA SN  Y+SV+HRV++ +  ER S A+F+CP   + I 
Sbjct: 241 GQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPANIERMSIAFFVCPYLETEID 300

Query: 309 SCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRF 342
               P  YR F+F EY++Q   DV++TG KVGLSRF
Sbjct: 301 CFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRF 334

[Close]
BLAST of Carg21988 vs. Swiss-Prot
Match: sp|Q9FFF6|DIOX5_ARATH (Probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Arabidopsis thaliana OX=3702 GN=At5g05600 PE=2 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 2.1e-39
Identity = 105/314 (33.44%), Postives = 160/314 (50.96%), Query Frame = 0 

Query: 27  AHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINH 86
           A L+P      PT++      +P++DL+GL S +          I EA   WGFFQV+NH
Sbjct: 46  ASLRPTTTEDAPTAT-----NIPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNH 105

Query: 87  GIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYR-WGAATATQPN-QFSWSEAF--HI 146
           G++ EL+    +   + F +P   K T      +Y  +G+    +      WS+ +  H+
Sbjct: 106 GVKPELMDAARENWREFFHMPVNAKETYSNSPRTYEGYGSRLGVEKGASLDWSDYYFLHL 165

Query: 147 PLTKVSETDCYGDF-IHLREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMC--DES 206
               + + + +  F   +REV++E    + KL+  +  VL  +LG +++ F++    +  
Sbjct: 166 LPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENI 225

Query: 207 TCFLRLNHYPICPFPGEVTGLVPHTDSDYLTI-LHQDSGGGLELMKGSQWVAVKPIPEAL 266
              LR+N+YP CP P    GL PH+D   +TI L  D   GL++ K   W+ VKP P A 
Sbjct: 226 GACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAF 285

Query: 267 VVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIG------SCRQPSP 326
           +VNIGD +Q  SN TYKSV+HRV+ NS +ER S A+F  P +   I       S   P  
Sbjct: 286 IVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAFFYNPKSDIPIQPLQELVSTHNPPL 345

[Close]
BLAST of Carg21988 vs. Swiss-Prot
Match: sp|Q9SRM3|DIOX6_ARATH (Probable 2-oxoglutarate-dependent dioxygenase At3g111800 OS=Arabidopsis thaliana OX=3702 GN=At3g11180 PE=2 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.8e-38
Identity = 101/297 (34.01%), Postives = 149/297 (50.17%), Query Frame = 0 

Query: 45  ECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLF 104
           +  +P++DL  L SG E ++    + I EA  EWGFFQVINHG++ EL+    +     F
Sbjct: 92  DINIPIIDLDSLFSGNEDDK----KRISEACREWGFFQVINHGVKPELMDAARETWKSFF 151

Query: 105 AVPFEKKSTSGILDNSYR-WGAATATQPNQ-FSWSEAFH---IPLTKVSETDCYGDFIHL 164
            +P E K        +Y  +G+    +      W++ ++   +PL             ++
Sbjct: 152 NLPVEAKEVYSNSPRTYEGYGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNI 211

Query: 165 REVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMC---DESTCFLRLNHYPICPFPGE 224
           RE+ +E    + KL   L  +L  +LG R E  ++     D   C LR+N+YP CP P  
Sbjct: 212 REMNDEYGKELVKLGGRLMTILSSNLGLRAEQLQEAFGGEDVGAC-LRVNYYPKCPQPEL 271

Query: 225 VTGLVPHTDSDYLTI-LHQDSGGGLELMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYK 284
             GL PH+D   +TI L  D   GL++  G  W+ V P+  A +VNIGD +Q  SN  YK
Sbjct: 272 ALGLSPHSDPGGMTILLPDDQVVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSKYK 331

Query: 285 SVKHRVVTNSVRERYSTAYFLCPSNHSGIGSCRQ------PSPYRHFTFGEYRKQVR 327
           SV+HRV+ NS +ER S A+F  P +   I   +Q      P  Y   TF +YR  +R
Sbjct: 332 SVEHRVIVNSEKERVSLAFFYNPKSDIPIQPMQQLVTSTMPPLYPPMTFDQYRLFIR 383

[Close]
BLAST of Carg21988 vs. TrEMBL
Match: tr|A0A0A0KIW0|A0A0A0KIW0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G052540 PE=3 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 8.1e-169
Identity = 285/344 (82.85%), Postives = 313/344 (90.99%), Query Frame = 0 

Query: 1   MFKEDTT-MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESG 60
           MFKE+TT MM+SNPPLF QYE LLQSTAHLQP +NG  PT+SV+EECQLPL+DLKGL+S 
Sbjct: 1   MFKENTTMMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSS 60

Query: 61  KEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDN 120
            EKER+AC REIFEAS EWGFFQVINHGI +ELL+RM+KEQ+KLF VPFEKK TSGILDN
Sbjct: 61  DEKERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDN 120

Query: 121 SYRWGAATATQPNQFSWSEAFHIPLTKVSETDCYGDFIHLREVMEELATAMSKLARSLAG 180
           SYRWG  TAT PNQFSWSEAFHIPLTKVSE  CYGDFI+LREVMEE+A+AMSK+AR LAG
Sbjct: 121 SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAG 180

Query: 181 VLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGG 240
           VLVES+G RKE+ ED+CDESTCFLRLNHYPICPF GEV+GLVPHTDSD+LTILHQDSGGG
Sbjct: 181 VLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGG 240

Query: 241 LELMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPS 300
           L++MKGSQW+AVKP P+ALVVNIGDLLQAWSNDTYKS KHRVV N +RERYS AYFLCPS
Sbjct: 241 LQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHRVVANPMRERYSIAYFLCPS 300

Query: 301 NHSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 344
            HS IGSCR+PSPYRHFTF EYRKQVRDDVEKTGYK+GLS FRL
Sbjct: 301 YHSQIGSCREPSPYRHFTFAEYRKQVRDDVEKTGYKIGLSNFRL 344

[Close]
BLAST of Carg21988 vs. TrEMBL
Match: tr|W9SYI1|W9SYI1_9ROSA (Gibberellin 2-beta-dioxygenase 8 OS=Morus notabilis OX=981085 GN=L484_014953 PE=3 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 5.5e-125
Identity = 219/336 (65.18%), Postives = 263/336 (78.27%), Query Frame = 0 

Query: 8   MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMAC 67
           +++SNPPL   Y+ LL  ++ LQ             EECQLP++DL GL+S +  ER+ C
Sbjct: 2   IIDSNPPLLDHYKALLDHSSTLQTAECDGHFDGVAAEECQLPMIDLNGLKSDEALERVEC 61

Query: 68  SREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAAT 127
           ++ I +A++EWGFFQV+NHGI  EL+ +M KEQ KLF  PFE K+TS +L+NSYRWG  T
Sbjct: 62  AKAISKAASEWGFFQVVNHGISLELVKKMRKEQTKLFEAPFEDKATSRLLNNSYRWGTPT 121

Query: 128 ATQPNQFSWSEAFHIPLTKVSETDCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGH 187
           AT+P+QFSWSEAFHIPLTKVSE DCYGDFI LRE  EE A AMSKLARSLAGVL E+LGH
Sbjct: 122 ATRPSQFSWSEAFHIPLTKVSEEDCYGDFISLREPTEEFAAAMSKLARSLAGVLAENLGH 181

Query: 188 RKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLELMKGSQ 247
           R++VFE++CDESTCFLRLN YP C    E+ GLVPHTDS +LTIL QD  GGL+LMK  +
Sbjct: 182 RRQVFEEICDESTCFLRLNRYPACAVSSEIFGLVPHTDSGFLTILRQDHVGGLQLMKDYK 241

Query: 248 WVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIGSC 307
           WV+VKP PEAL+VNIGDL QAWSND +KSV+HRV+TN + ERYS AYFLCPS  S IGSC
Sbjct: 242 WVSVKPNPEALIVNIGDLFQAWSNDVFKSVEHRVMTNKM-ERYSIAYFLCPSYDSLIGSC 301

Query: 308 RQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 344
           R+PS YR FTF +YR QV++DV+K GYKVGLSRF L
Sbjct: 302 REPSVYRKFTFRQYRSQVQEDVKKMGYKVGLSRFLL 336

[Close]
BLAST of Carg21988 vs. TrEMBL
Match: tr|A0A1S3C0F9|A0A1S3C0F9_CUCME (gibberellin 2-beta-dioxygenase 8-like OS=Cucumis melo OX=3656 GN=LOC103495059 PE=3 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 7.2e-125
Identity = 215/262 (82.06%), Postives = 239/262 (91.22%), Query Frame = 0 

Query: 6   TTMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERM 65
           TTMM+SNPPLF QYE LLQSTAHLQP +NG  PT+SV+EECQLPL+DLKGL+S  EKER+
Sbjct: 48  TTMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSNEKERV 107

Query: 66  ACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGA 125
           AC REIFEAS EWGFFQVINHGI +ELL+RM+KEQ+KLF VPFEKK TSGILDNSYRWG 
Sbjct: 108 ACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKITSGILDNSYRWGT 167

Query: 126 ATATQPNQFSWSEAFHIPLTKVSETDCYGDFIHLREVMEELATAMSKLARSLAGVLVESL 185
            TAT PNQ SWSEAFHIPLTKVSE  CYGDFI+LREVMEE+A+AMSK+AR LAGVLVES+
Sbjct: 168 PTATHPNQLSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLVESM 227

Query: 186 GHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLELMKG 245
           G RKE+ ED+CDESTCFLRLNHYPICPF GEV+GLVPHTDSD+LTILHQDSGGGL++MKG
Sbjct: 228 GQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKG 287

Query: 246 SQWVAVKPIPEALVVNIGDLLQ 268
           SQW+AVKP P+ALVVNIGDLLQ
Sbjct: 288 SQWLAVKPNPQALVVNIGDLLQ 309

[Close]
BLAST of Carg21988 vs. TrEMBL
Match: tr|A0A2P4JVF1|A0A2P4JVF1_QUESU (Gibberellin 2-beta-dioxygenase 8 OS=Quercus suber OX=58331 GN=CFP56_69756 PE=3 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 1.6e-124
Identity = 218/336 (64.88%), Postives = 258/336 (76.79%), Query Frame = 0 

Query: 8   MMNSNPPLFQQYELLLQSTAHLQP-LHNGYQPTSSVLEECQLPLVDLKGL-ESGKEKERM 67
           M   NPPL Q Y  L +    + P L    +  + V+EECQLPL+DL  L +   E+ER+
Sbjct: 1   MTEPNPPLLQHYGALFRHPVEVPPALEFDERQNAPVMEECQLPLIDLSALIKHHNERERL 60

Query: 68  ACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGA 127
           ACS  I +AS+EWGFFQV+NHGI  ELL  M KEQ+KLF  PFE K++ G+L+NSYRWG 
Sbjct: 61  ACSTAICKASSEWGFFQVVNHGISPELLGTMKKEQLKLFQTPFESKASCGLLNNSYRWGT 120

Query: 128 ATATQPNQFSWSEAFHIPLTKVSETDCYGDFIHLREVMEELATAMSKLARSLAGVLVESL 187
            TAT P QFSWSEAFHIPLTK+SE  CYG+F  LREVM+E A AMS+LAR LAG+L E+L
Sbjct: 121 PTATYPKQFSWSEAFHIPLTKISEEACYGEFSSLREVMKEFAAAMSELARLLAGILAENL 180

Query: 188 GHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLELMKG 247
           GH+K VFED+CDESTCF+RLN YP CP   E+ GLVPHTDSD+LTIL+QD  GGL+LMK 
Sbjct: 181 GHQKGVFEDICDESTCFIRLNRYPACPLSPEMFGLVPHTDSDFLTILYQDQVGGLQLMKD 240

Query: 248 SQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIG 307
           S+WVAVKP  +AL+VNIGDL QAWSND YKSV+H+V+ N   ERYS AYFLCPS  S IG
Sbjct: 241 SKWVAVKPNQDALIVNIGDLFQAWSNDVYKSVEHKVMANRTTERYSIAYFLCPSYDSLIG 300

Query: 308 SCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRF 342
           SCR+PS YR FTFGEYR QV++DV+K GYKVGL RF
Sbjct: 301 SCREPSIYRKFTFGEYRSQVQEDVKKNGYKVGLPRF 336

[Close]
BLAST of Carg21988 vs. TrEMBL
Match: tr|A0A2I4G9F7|A0A2I4G9F7_9ROSI (gibberellin 2-beta-dioxygenase 8-like OS=Juglans regia OX=51240 GN=LOC109005901 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 2.1e-124
Identity = 222/338 (65.68%), Postives = 262/338 (77.51%), Query Frame = 0 

Query: 8   MMNSNPPLFQQYELLLQSTA-HLQPL-HNGYQPTSSVLEECQLPLVDLKGLESGKEKERM 67
           M+ SNPPL   Y+ LL  TA  +  L +       +V+EECQLPL+DL GL+     ER+
Sbjct: 1   MLESNPPLLPHYKALLSHTADEVAALEYCNVWKGGAVMEECQLPLIDLDGLKCLDATERL 60

Query: 68  ACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGA 127
           ACS  I  AS+EWGFFQV+NHGI SELL +M  EQ+KLF  PFE K T G+L+NSYRWG 
Sbjct: 61  ACSTAICRASSEWGFFQVVNHGISSELLGKMRGEQIKLFQAPFEMKQTCGLLNNSYRWGT 120

Query: 128 ATATQPNQFSWSEAFHIPLTKVSETDCYGDFIHLREVMEELATAMSKLARSLAGVLVESL 187
            TAT P QFSWSEAFHIPLTK+SE  CYG+F  LR V++E A AM+KLAR LAGVL E+L
Sbjct: 121 PTATLPTQFSWSEAFHIPLTKISEEACYGEFGSLRGVLKEFAEAMAKLARLLAGVLTENL 180

Query: 188 GHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLELMKG 247
           G+RKEV ED+CDESTCFLRLN YP CP   E+ GLVPHTDSD+LTIL+QD  GGL+LMK 
Sbjct: 181 GNRKEVIEDICDESTCFLRLNRYPACPISPEIFGLVPHTDSDFLTILYQDQVGGLQLMKD 240

Query: 248 SQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIG 307
           S+WVAVKP  +AL+VNIGDL QAWSND YKSV+H+V+ N+ RERYS AYFLCPS  S IG
Sbjct: 241 SKWVAVKPNQDALIVNIGDLFQAWSNDVYKSVEHKVMANTKRERYSIAYFLCPSYDSLIG 300

Query: 308 SCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 344
           SCR+PS YR FTFGEYRK+V++DV++ GYKVGL RF L
Sbjct: 301 SCREPSVYRKFTFGEYRKKVQEDVKRDGYKVGLPRFLL 338

The following BLAST results are available for this feature:
BLAST of Carg21988 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022942818.12.3e-19999.13gibberellin 2-beta-dioxygenase 8-like [Cucurbita moschata][more]
XP_023514448.11.5e-19599.11gibberellin 2-beta-dioxygenase 8-like [Cucurbita pepo subsp. pepo][more]
XP_022985325.17.1e-19395.92gibberellin 2-beta-dioxygenase 8-like [Cucurbita maxima][more]
XP_022942053.13.2e-16985.00gibberellin 2-beta-dioxygenase 8-like, partial [Cucurbita moschata][more]
XP_004142323.21.2e-16882.85PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus] >KGN49660.1 h... [more]
BLAST of Carg21988 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G21200.14.3e-9152.48gibberellin 2-oxidase 8[more]
AT1G50960.19.3e-7041.67gibberellin 2-oxidase 7[more]
AT3G19010.17.4e-4334.192-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT3G19000.12.8e-4234.112-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT5G58660.14.8e-4234.042-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
BLAST of Carg21988 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q7XP65|G2OX6_ORYSJ5.0e-9756.07Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2... [more]
sp|O49561|G2OX8_ARATH7.7e-9052.48Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ... [more]
sp|Q9C6I4|G2OX7_ARATH1.7e-6841.67Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ... [more]
sp|Q9FFF6|DIOX5_ARATH2.1e-3933.44Probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Arabidopsis thaliana ... [more]
sp|Q9SRM3|DIOX6_ARATH1.8e-3834.01Probable 2-oxoglutarate-dependent dioxygenase At3g111800 OS=Arabidopsis thaliana... [more]
BLAST of Carg21988 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0KIW0|A0A0A0KIW0_CUCSA8.1e-16982.85Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G052540 PE=3 SV=1[more]
tr|W9SYI1|W9SYI1_9ROSA5.5e-12565.18Gibberellin 2-beta-dioxygenase 8 OS=Morus notabilis OX=981085 GN=L484_014953 PE=... [more]
tr|A0A1S3C0F9|A0A1S3C0F9_CUCME7.2e-12582.06gibberellin 2-beta-dioxygenase 8-like OS=Cucumis melo OX=3656 GN=LOC103495059 PE... [more]
tr|A0A2P4JVF1|A0A2P4JVF1_QUESU1.6e-12464.88Gibberellin 2-beta-dioxygenase 8 OS=Quercus suber OX=58331 GN=CFP56_69756 PE=3 S... [more]
tr|A0A2I4G9F7|A0A2I4G9F7_9ROSI2.1e-12465.68gibberellin 2-beta-dioxygenase 8-like OS=Juglans regia OX=51240 GN=LOC109005901 ... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: INTERPRO
TermDefinition
IPR026992DIOX_N
IPR027443IPNS-like
IPR005123Oxoglu/Fe-dep_dioxygenase
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016491 oxidoreductase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg21988-RACarg21988-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 202..298
e-value: 1.0E-25
score: 90.0
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 197..298
score: 14.395
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 46..337
e-value: 9.2E-83
score: 280.2
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 48..142
e-value: 1.5E-16
score: 61.1
NoneNo IPR availablePANTHERPTHR10209:SF177GIBBERELLIN 2-BETA-DIOXYGENASE 7coord: 23..342
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 23..342
NoneNo IPR availableSUPERFAMILYSSF51197Clavaminate synthase-likecoord: 33..325