CmaCh04G000330 (gene) Cucurbita maxima (Rimu)

Overview
NameCmaCh04G000330
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationCma_Chr04 : 191407 .. 194668 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGGCCATATGTTTAAGGAAGATACCACAGTGATGAACTCCAATCCACCACTCTTCCATCAATATGGGTTGCTTTTGCAATCCACAGCTCATTTACAGCCACTTCAAAGTGGCTATCAACCAACTAGCTCTGTCCTGGAGGAATGTCAGCTCCCTCTTGTCGATCTTAAAGGTTTGGAAAGTGGCAAAGAAAAAGAGAGGATGGCTTGCAGTAGCGAGATTTTTGAAGCATCTGCAGAATGGGGATTTTTCCAAGTGATTAACCATGGGATACGCTCAGAGCTGCTGAGCAGAATGAGCAAAGAACAAATGAAGTTGTTTGCGGTGCCCTTTGAGAAAAAATCTACAAGTGGGATTTTGGATAATTCTTACAGATGGGGAGCAGCCACTGCAACCCAACCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGACTGCTATGGCAACTTCATTCACTTAAGGTATAGATTTTATCACCCTTCTTCCAACTAGAAACTTCTCTTGAGCATTGTCTCAAATTTATTTAGTTCATATGTTTAAAACACAGTCTATTTAGCCTTTGAACTTACAAAAAAAAAAAAAAAAAAAAATCCCATTTCATTCTAGGTTATGTTTTTCTTTGTTAACATCAGAATGCTTAATATGACAGGCTGTTCGCCTGTCCAGTTGTTTGCTATAATATCGCACCAACTCAGCTAAGAGTCATATAAAAACATGTCACAATAGCGTTCCTTGATAGTTTCAATGACAATCACTAAACAAAGCATAATTCAAAATTTAACAATATAATAAAACAAATCCAAAACCCAACGTCTAAGGACTATACTCGTATTCTTTAACATCGTAATATTCACGCACTTGAAGATTTTAGGTCTTCTAGACATTTTCACAAACAGTTCGACAGCAAAAGTAATGCACTCCGATATCACAATCAACCAATGTACTAGATTACAAAAACAAAGAGAGTGCATTGCTCACTGACCTTATATCCTCGAACGCCAGGGAAGTAATGGAGGAACTGGCAACCGCCATGTCGAAGCTCGCAAGATCTCTAGCAGGAGTTTTAGTGGAGAGCTTGGGCCACCGGAAGGAAGTATTTGAAGATATGTGCGATGAGAGCACATGCTTCCTCCGGTTGAATCACTACCCAATCTGTCCGTTTCCCGGCAAAGTTACCGGCCTGGTGCCCCACACCGATAGCGATTATCTGACCATACTTCATCAAGATTCCGGCGGTGGGCTTCAGCTGATGAAAGGGTCGCAATGGGTGGCCGTGAAACCCATTCCGGAAGCCCTTGTCGTCAACATTGGAGATCTTCTACAGGTACAAACTGAAACTCTTTCGAAGTATAATGGTTTGGTAGAGGAAGTAGAAATTGGGTATAAAAATATAGTTTGAAATGCAGGCTTGGAGCAACGATACGTACAAGAGTGTGAAGCATAGGGTGGTGGCTAACTCGGCGCGGGAAAGATATTCGACAGCATACTTCCTCTGCCCATCTGACCATTCTGGAATCGGGAGTTGCCGGCAGCCTTCACCATACAGACACTTCACATTTGGAGAGTACAGGAAGCAAGTGAGAGATGATGTGGAAAAAACTGGGTACAAAGAAGGGCTTTCCAGGTTTCGGCTGTAAATCACTCCAATAACAAATGGAAGTATCAATTTCAAGTACGCATAAACATCCAAACGATTAAGAACAATCACGTGCATCCGTATCTATCAAGGCTTTAAAAATAAGGGTTTAACCAGTACACATCTACATATCTAATCAGTGAGCTAATGCCACTTCCAATCTAATAAGAAGGTGAAGGGTTAAGATTCTGAAGAGCAATAGGAGAGATAGGCGAGCAAGCTACAGAGACTAGAAACAAAAGAATGCAAATTAGGAAGAACCAATGCTATCTAAAATGTGGGCGTTCCAACAACAAAAGAATTCCAAAAGATTGTTAAGTAGCTGATTTGATGTTTTACTCTTTGAACTTTTGTTATATGAATGTAATGAAGTTTTATTTCGTCTCTCATCATTATTTGTTTATATATCTATGCGACTAGTATGGATACCTTTATGTTTTTCGGATTTTATATAAAATAGAAAAAAATTATAACCAACACCTCTTAAATTCCTTTTAAAATTATATAAATTAAACTTTTTAACTTTTTTATTTAAATTATAGGTCAATTTTATAATTTAACATCTTATTTTCTATTTATTTTCCCTCTTATTTATTATTTTTAATTGCTTTTAACCCGCGTTCCGCCGCCTCCTCCTCCCTCCCATTTCCAGCCGCTGCTTCCCGTCCCTCCGCACGTCGTTCCTGCCCGACCCCATTTCTGCAGCTTCCGCTGCAAGCTACAGTGCCAGCCTTCTGTGAAATTGCCGCTGCCCCTGTCTCTCCGAGCAAACAGAGTAAGTTCTCTGTTTTTTTTCTTCTAAGAGACACAGCGGTTCAACCCTTAACCGCATCGCCGCCACTCATTTTTCAAAACCCAAGGTCTTATCTTCTGTTTTGTTTTTTAATCTGTTTCTTAGGGTTACGTTGATTTATTCTTTCACCGACGAAGAAGAACATCAATAACTTTTTCTTTTCTTCGTTGATTTTATCTTCAACCAATGTTGTATTAGTCCAAATGAAAACGTAAAACTAAGAACGTAAACCGATTGATCAATAAAACAAACTATAATTCTTATCCGAGTTAATTTATAAAGAGGAGACAATAACTCACCTTTAGTTTGGTTGGCTATCAAAAACTCGTTGGATCGAGATGTGGCATTTGAAAAGATTGAAATTAATGTTTATCGGTCGAAAAACTAATTGAGTTTGAGTTTGATTGAATGGCGTTGAATCTTCTTGAATATAGAAGAAACTATGGATGCCCATGAACTGGCATAGGGAGTTCCAAATCACAGCTGACAACAATTTTTTTTCTTGCATCCAAACACGTTCAAGGATTTCGAATTAGAACTGATTTGAAATCCTTCCAAATATAGAGGTTCAAACAGCGGTAAAGGAGAATAGATAAAGGTTACTGAAACCCTCGCCCCTTCTTTTTCCCTCTCCCTTGAGTTTTGATTTTGTTTAATAATGGGAAAGTCTGTTTCTGCTGCAACTTCCTGAAAATTTTATGCTGTTACGTTATATATATATATATATATATATATATGCATCAAACAGTCAATATTCATGGCGCAAGATAATATCAGGGAGAAACAGATGGTGAGAGTTGCTTCTGA

mRNA sequence

AAGGCCATATGTTTAAGGAAGATACCACAGTGATGAACTCCAATCCACCACTCTTCCATCAATATGGGTTGCTTTTGCAATCCACAGCTCATTTACAGCCACTTCAAAGTGGCTATCAACCAACTAGCTCTGTCCTGGAGGAATGTCAGCTCCCTCTTGTCGATCTTAAAGGTTTGGAAAGTGGCAAAGAAAAAGAGAGGATGGCTTGCAGTAGCGAGATTTTTGAAGCATCTGCAGAATGGGGATTTTTCCAAGTGATTAACCATGGGATACGCTCAGAGCTGCTGAGCAGAATGAGCAAAGAACAAATGAAGTTGTTTGCGGTGCCCTTTGAGAAAAAATCTACAAGTGGGATTTTGGATAATTCTTACAGATGGGGAGCAGCCACTGCAACCCAACCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGACTGCTATGGCAACTTCATTCACTTAAGGGAAGTAATGGAGGAACTGGCAACCGCCATGTCGAAGCTCGCAAGATCTCTAGCAGGAGTTTTAGTGGAGAGCTTGGGCCACCGGAAGGAAGTATTTGAAGATATGTGCGATGAGAGCACATGCTTCCTCCGGTTGAATCACTACCCAATCTGTCCGTTTCCCGGCAAAGTTACCGGCCTGGTGCCCCACACCGATAGCGATTATCTGACCATACTTCATCAAGATTCCGGCGGTGGGCTTCAGCTGATGAAAGGGTCGCAATGGGTGGCCGTGAAACCCATTCCGGAAGCCCTTGTCGTCAACATTGGAGATCTTCTACAGGCTTGGAGCAACGATACGTACAAGAGTGTGAAGCATAGGGTGGTGGCTAACTCGGCGCGGGAAAGATATTCGACAGCATACTTCCTCTGCCCATCTGACCATTCTGGAATCGGGAGTTGCCGGCAGCCTTCACCATACAGACACTTCACATTTGGAGAGTACAGGAAGCAACCGCTGCTTCCCGTCCCTCCGCACGTCGTTCCTGCCCGACCCCATTTCTGCAGCTTCCGCTGCAAGCTACAGTGCCAGCCTTCTGTGAAATTGCCGCTGCCCCTGTCTCTCCGAGCAAACAGAGGAGAAACAGATGGTGAGAGTTGCTTCTGA

Coding sequence (CDS)

ATGTTTAAGGAAGATACCACAGTGATGAACTCCAATCCACCACTCTTCCATCAATATGGGTTGCTTTTGCAATCCACAGCTCATTTACAGCCACTTCAAAGTGGCTATCAACCAACTAGCTCTGTCCTGGAGGAATGTCAGCTCCCTCTTGTCGATCTTAAAGGTTTGGAAAGTGGCAAAGAAAAAGAGAGGATGGCTTGCAGTAGCGAGATTTTTGAAGCATCTGCAGAATGGGGATTTTTCCAAGTGATTAACCATGGGATACGCTCAGAGCTGCTGAGCAGAATGAGCAAAGAACAAATGAAGTTGTTTGCGGTGCCCTTTGAGAAAAAATCTACAAGTGGGATTTTGGATAATTCTTACAGATGGGGAGCAGCCACTGCAACCCAACCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGACTGCTATGGCAACTTCATTCACTTAAGGGAAGTAATGGAGGAACTGGCAACCGCCATGTCGAAGCTCGCAAGATCTCTAGCAGGAGTTTTAGTGGAGAGCTTGGGCCACCGGAAGGAAGTATTTGAAGATATGTGCGATGAGAGCACATGCTTCCTCCGGTTGAATCACTACCCAATCTGTCCGTTTCCCGGCAAAGTTACCGGCCTGGTGCCCCACACCGATAGCGATTATCTGACCATACTTCATCAAGATTCCGGCGGTGGGCTTCAGCTGATGAAAGGGTCGCAATGGGTGGCCGTGAAACCCATTCCGGAAGCCCTTGTCGTCAACATTGGAGATCTTCTACAGGCTTGGAGCAACGATACGTACAAGAGTGTGAAGCATAGGGTGGTGGCTAACTCGGCGCGGGAAAGATATTCGACAGCATACTTCCTCTGCCCATCTGACCATTCTGGAATCGGGAGTTGCCGGCAGCCTTCACCATACAGACACTTCACATTTGGAGAGTACAGGAAGCAACCGCTGCTTCCCGTCCCTCCGCACGTCGTTCCTGCCCGACCCCATTTCTGCAGCTTCCGCTGCAAGCTACAGTGCCAGCCTTCTGTGAAATTGCCGCTGCCCCTGTCTCTCCGAGCAAACAGAGGAGAAACAGATGGTGAGAGTTGCTTCTGA

Protein sequence

MFKEDTTVMNSNPPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESGKEKERMACSSEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGNFIHLREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGGLQLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVANSARERYSTAYFLCPSDHSGIGSCRQPSPYRHFTFGEYRKQPLLPVPPHVVPARPHFCSFRCKLQCQPSVKLPLPLSLRANRGETDGESCF
Homology
[Close]
BLAST of CmaCh04G000330 vs. Swiss-Prot
Match: G2OX8_ARATH (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 303.9 bits (777), Expect = 2.4e-81
Identity = 147/287 (51.22%), Postives = 197/287 (68.64%), Query Frame = 1 

Query: 40  SSVLEECQLPLVDLKGLESGKEKERMACSSEIFEASAEWGFFQVINHGIRSELLSRMSKE 99
           ++V+EE +LP++D+  L  G E+ER  C   I  AS EWGFFQVINHGI  ++L +M +E
Sbjct: 34  TAVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQE 93

Query: 100 QMKLFAVPFEKKSTSGILD-NSYRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGNFIH 159
           Q+++F  PF+KKS S      SYRWG  +AT   Q SWSEAFH+P+T +S+     +F  
Sbjct: 94  QIRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFTT 153

Query: 160 LREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGKVT 219
           L   ME+ A+    LA  LA VL E  G     F++ C  +TC+LR+N YP CP P +V 
Sbjct: 154 LSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVY 213

Query: 220 GLVPHTDSDYLTILHQDSGGGLQLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVK 279
           GL+PHTDSD+LTIL+QD  GGLQL+K ++W+AVKP P+AL++NIGDL QAWSN  YKSV+
Sbjct: 214 GLMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVE 273

Query: 280 HRVVANSARERYSTAYFLCPSDHSGIGSCRQPSP-YRHFTFGEYRKQ 325
           HRV+ N   ER+STAYF+CPS +  +  C    P YR+F+F E+R+Q
Sbjct: 274 HRVMTNPKVERFSTAYFMCPS-YDAVIECSSDRPAYRNFSFREFRQQ 316

[Close]
BLAST of CmaCh04G000330 vs. Swiss-Prot
Match: G2OX7_ARATH (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 5.6e-62
Identity = 129/319 (40.44%), Postives = 185/319 (57.99%), Query Frame = 1 

Query: 9   MNSNPPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESGKEKERMACS 68
           M S PP    +  +  S+      +S    ++      +LP++DL  L SG+E +R  C 
Sbjct: 1   MASQPPFKTNFCSIFGSSFPNSTSESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCV 60

Query: 69  SEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILD---NSYRWGA 128
            ++  A+ EWGFFQ++NHGI  ++   M  E+ KLF  PF  K      D   NSYRWG 
Sbjct: 61  KQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQPFSVKVRERFSDLSKNSYRWGN 120

Query: 129 ATATQPNQFSWSEAFHIPLTKVSEADCYGNFIHLREVMEELATAMSKLARSLAGVLVESL 188
            +AT P Q+S SEAFHI L++VS      N  +LR ++E     ++++A+ +  +L + +
Sbjct: 121 PSATSPAQYSVSEAFHIILSEVSRISDDRN--NLRTIVETYVQEIARVAQMICEILGKQV 180

Query: 189 GHRKEVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGGLQLMKG 248
               E FE++ +    FLRLN Y    F  +V GLVPHTD+ +LTIL QD  GGL+L   
Sbjct: 181 NVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENN 240

Query: 249 SQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVANSARERYSTAYFLCPSDHSGIG 308
            QW++VKP  EAL VNIGD+ QA SN  Y+SV+HRV++ +  ER S A+F+CP   + I 
Sbjct: 241 GQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPANIERMSIAFFVCPYLETEID 300

Query: 309 SCRQPSPYRHFTFGEYRKQ 325
               P  YR F+F EY++Q
Sbjct: 301 CFGYPKKYRRFSFREYKEQ 317

[Close]
BLAST of CmaCh04G000330 vs. Swiss-Prot
Match: GAOX3_ARATH (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana GN=GA20OX3 PE=2 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.6e-37
Identity = 106/319 (33.23%), Postives = 156/319 (48.90%), Query Frame = 1 

Query: 3   KEDTTVMNSNPPLFHQYGLLLQSTAHL--QPLQSGYQPTSSVLEECQLPLVDLKGLESGK 62
           KED+++ ++         LL Q + H+  Q +   ++  S+ ++  Q+PL+DL G  SG 
Sbjct: 19  KEDSSIFDAK--------LLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGD 78

Query: 63  EKERMACSSEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNS 122
                  +  + +A+ + GFF + NHG+   LLSR        F  P  +K  +      
Sbjct: 79  SCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACEKQKA-----Q 138

Query: 123 YRWGAATA-----------------TQPNQFSWSEAFHIPLTK--VSE--ADCYGNFIHL 182
            +WG ++                  T   +FS  E  H    K  VS+   D Y +F   
Sbjct: 139 RKWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIHSQTVKDFVSKKMGDGYEDF--- 198

Query: 183 REVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGKVTG 242
            +V +E A AM+ L+  +  +L  SLG  +  F++  ++S    RLN+YP C  P    G
Sbjct: 199 GKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELALG 258

Query: 243 LVPHTDSDYLTILHQDSGGGLQLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKH 299
             PH D   LTILHQD  GGLQ+   ++W ++ P P A VVNIGD   A +N  YKS  H
Sbjct: 259 TGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYKSCLH 318

[Close]
BLAST of CmaCh04G000330 vs. Swiss-Prot
Match: GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 157.5 bits (397), Expect = 2.8e-37
Identity = 105/300 (35.00%), Postives = 149/300 (49.67%), Query Frame = 1 

Query: 13  PPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESGKEKERMACSSEIF 72
           P +F+   L LQ+    Q +    +  S  + E  +PL+DL+ L S      +  S  I 
Sbjct: 26  PLIFNPSMLNLQANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLSDPSST-LDASRLIS 85

Query: 73  EASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAATATQPN 132
           EA  + GFF V+NHGI  EL+S   +   + F +P  +K    +L  S       ++   
Sbjct: 86  EACKKHGFFLVVNHGISEELISDAHEYTSRFFDMPLSEKQR--VLRKSGESVGYASSFTG 145

Query: 133 QFS----WSEA----FHIPLTKVSEADCY------GNFIHLREVMEELATAMSKLARSLA 192
           +FS    W E     F   +++      Y        F    +V +E   AMS L+  + 
Sbjct: 146 RFSTKLPWKETLSFRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIM 205

Query: 193 GVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGG 252
            +L  SLG +++ F +  +E+   +RLN+YP C  P    G  PH D   LTILHQD   
Sbjct: 206 ELLGLSLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVN 265

Query: 253 GLQLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVANSARERYSTAYFLCP 299
           GLQ+   +QW +++P P+A VVNIGD   A SND YKS  HR V NS  ER S A+FLCP
Sbjct: 266 GLQVFVENQWRSIRPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCP 322

[Close]
BLAST of CmaCh04G000330 vs. Swiss-Prot
Match: GAO1B_WHEAT (Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 6.9e-36
Identity = 95/274 (34.67%), Postives = 133/274 (48.54%), Query Frame = 1 

Query: 35  GYQPTSSVLEECQLPLVDLKGLESGKEKERMACSSEIFEASAEWGFFQVINHGIRSELLS 94
           G  PT    EE  +PL+D+ G+ SG  +     +  + EA    GFFQV+NHGI +ELL+
Sbjct: 26  GESPTPDAAEELHVPLIDIGGMLSGDPRATAEVTRLVGEACERHGFFQVVNHGIDAELLA 85

Query: 95  RMSKEQMKLFAVPF-EKKSTSGILDNSYRWGAA-TATQPNQFSWSEAFHIPLTKVSEADC 154
              +     F +P  EK+        S  + ++ T    ++  W E           A  
Sbjct: 86  DAHRCVDAFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKETLSFRSCPSDPALV 145

Query: 155 YGNFI--------HLREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLR 214
               +         L EV     + MS+L+  +  VL ESLG  +  +    + +   +R
Sbjct: 146 VDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAHYRRFFEGNDSIMR 205

Query: 215 LNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGGLQLMKGSQWVAVKPIPEALVVNIGD 274
           LN+YP C  P +  G  PH D   LTILHQD+ GGLQ+    +W +++P  +A VVNIGD
Sbjct: 206 LNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPRADAFVVNIGD 265

Query: 275 LLQAWSNDTYKSVKHRVVANSARERYSTAYFLCP 299
              A SN  YKS  HR V NS   R S A+FLCP
Sbjct: 266 TFMALSNGRYKSCLHRAVVNSKVPRKSLAFFLCP 299

[Close]
BLAST of CmaCh04G000330 vs. TrEMBL
Match: A0A0A0KIW0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G052540 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 6.0e-156
Identity = 265/325 (81.54%), Postives = 293/325 (90.15%), Query Frame = 1 

Query: 1   MFKEDTTVM-NSNPPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESG 60
           MFKE+TT+M +SNPPLF QY  LLQSTAHLQP  +G  PT+SV+EECQLPL+DLKGL+S 
Sbjct: 1   MFKENTTMMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSS 60

Query: 61  KEKERMACSSEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDN 120
            EKER+AC  EIFEAS EWGFFQVINHGI +ELL+RM+KEQ+KLF VPFEKK TSGILDN
Sbjct: 61  DEKERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDN 120

Query: 121 SYRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGNFIHLREVMEELATAMSKLARSLAG 180
           SYRWG  TAT PNQFSWSEAFHIPLTKVSEA CYG+FI+LREVMEE+A+AMSK+AR LAG
Sbjct: 121 SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAG 180

Query: 181 VLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGG 240
           VLVES+G RKE+ ED+CDESTCFLRLNHYPICPF G+V+GLVPHTDSD+LTILHQDSGGG
Sbjct: 181 VLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGG 240

Query: 241 LQLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVANSARERYSTAYFLCPS 300
           LQ+MKGSQW+AVKP P+ALVVNIGDLLQAWSNDTYKS KHRVVAN  RERYS AYFLCPS
Sbjct: 241 LQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHRVVANPMRERYSIAYFLCPS 300

Query: 301 DHSGIGSCRQPSPYRHFTFGEYRKQ 325
            HS IGSCR+PSPYRHFTF EYRKQ
Sbjct: 301 YHSQIGSCREPSPYRHFTFAEYRKQ 325

[Close]
BLAST of CmaCh04G000330 vs. TrEMBL
Match: F6HG07_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g01650 PE=3 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 7.2e-117
Identity = 205/318 (64.47%), Postives = 245/318 (77.04%), Query Frame = 1 

Query: 7   TVMNSNPPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESGKEKERMA 66
           T+ NSNPPL   YG+L Q        Q       + +EECQLPL+DL+GL S  E+ER+A
Sbjct: 23  TMTNSNPPLLQHYGVLCQHGETPAVQQRDCGNNGAAMEECQLPLIDLEGLWSESEEERLA 82

Query: 67  CSSEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAA 126
           C+S I  AS++WGFFQV+NHGIR ELLS M +EQ+KLF  PFE+K+   +LDNSYRWG  
Sbjct: 83  CASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWGTP 142

Query: 127 TATQPNQFSWSEAFHIPLTKVSEADCYGNFIHLREVMEELATAMSKLARSLAGVLVESLG 186
           TAT P + SWSEAFHIPLTKVSE  CYG F  LREVM+E A AMS LAR LAGVL ESLG
Sbjct: 143 TATCPKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAESLG 202

Query: 187 HRKEVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGGLQLMKGS 246
           H+K VF+++CDE+TCFLRLN YP CP   +V GLVPHTDSD+LTIL+QD  GGLQLMK S
Sbjct: 203 HQKGVFDEICDENTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMKDS 262

Query: 247 QWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVANSARERYSTAYFLCPSDHSGIGS 306
           +WVAVKP  + L+VNIGDL QAWSN+ YKSV+H+V+AN+ +ERYS AYFLCPS  + IGS
Sbjct: 263 KWVAVKPNKDTLIVNIGDLFQAWSNNEYKSVEHQVMANAIKERYSIAYFLCPSYDAFIGS 322

Query: 307 CRQPSPYRHFTFGEYRKQ 325
           C +P+ YR FTFGEYR+Q
Sbjct: 323 CSEPTIYRKFTFGEYRQQ 340

[Close]
BLAST of CmaCh04G000330 vs. TrEMBL
Match: B9RY49_RICCO (Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_0814470 PE=3 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.2e-116
Identity = 210/317 (66.25%), Postives = 244/317 (76.97%), Query Frame = 1 

Query: 8   VMNSNPPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESGKEKERMAC 67
           ++ SNPPL   Y  L       +PL+  + P S  +EECQLPLVDL  L+SG EKER AC
Sbjct: 1   MVESNPPLQLSYTALTHQIP--EPLER-HTPQSG-MEECQLPLVDLNCLKSGAEKERSAC 60

Query: 68  SSEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAAT 127
           SS I  AS+EWGFFQV+NHGI  ELL  M +EQ+KLF  PF +K+T G+L+NSYRWG  T
Sbjct: 61  SSAISTASSEWGFFQVVNHGISPELLKNMRREQVKLFQTPFHRKATCGLLNNSYRWGTPT 120

Query: 128 ATQPNQFSWSEAFHIPLTKVSEADCYGNFIHLREVMEELATAMSKLARSLAGVLVESLGH 187
           AT PNQFSWSEAFHIPLTK+SE  CYG F  LREVM E A AMSKLA+ LAG+L E+LGH
Sbjct: 121 ATCPNQFSWSEAFHIPLTKISEQACYGEFTSLREVMMEFAAAMSKLAKLLAGILAENLGH 180

Query: 188 RKEVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGGLQLMKGSQ 247
            + VFE++C ES CFLRLN YP CP   ++ GLVPHTDSD+LTIL+QD  GGLQLMK S+
Sbjct: 181 PRAVFEEICHESNCFLRLNRYPSCPISPEMFGLVPHTDSDFLTILYQDEVGGLQLMKDSK 240

Query: 248 WVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVANSARERYSTAYFLCPSDHSGIGSC 307
           WVAVKP PEAL+VNIGDL QAWSND YKSV+H+V+AN   ERYS AYFLCPS  S IGSC
Sbjct: 241 WVAVKPNPEALIVNIGDLFQAWSNDIYKSVEHKVMANGKMERYSVAYFLCPSYDSLIGSC 300

Query: 308 RQPSPYRHFTFGEYRKQ 325
           ++PS YR FTFGEYR+Q
Sbjct: 301 KEPSIYREFTFGEYREQ 313

[Close]
BLAST of CmaCh04G000330 vs. TrEMBL
Match: A5BF50_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_025459 PE=3 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 1.0e-115
Identity = 204/315 (64.76%), Postives = 242/315 (76.83%), Query Frame = 1 

Query: 10  NSNPPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESGKEKERMACSS 69
           NSNPPL   YG+L Q        Q       + +EECQLPL+DL+GL S  E+ER+AC+S
Sbjct: 3   NSNPPLLQHYGVLCQHGETPAVQQRDCGNNGAAMEECQLPLIDLEGLWSESEEERLACAS 62

Query: 70  EIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAATAT 129
            I  AS++WGFFQV+NHGIR ELLS M +EQ+KLF  PFE+K+   +LDNSYRWG  TAT
Sbjct: 63  AIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWGTPTAT 122

Query: 130 QPNQFSWSEAFHIPLTKVSEADCYGNFIHLREVMEELATAMSKLARSLAGVLVESLGHRK 189
           +  + SWSEAFHIPLTKVSE  CYG F  LREVM+E A AMS LAR LAGVL ESLGH+K
Sbjct: 123 RLKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAESLGHQK 182

Query: 190 EVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGGLQLMKGSQWV 249
            VF+++CDESTCFLRLN YP CP   +V GLVPHTDSD+LTIL+QD  GGLQLMK S+WV
Sbjct: 183 GVFDEICDESTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMKDSKWV 242

Query: 250 AVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVANSARERYSTAYFLCPSDHSGIGSCRQ 309
           AVKP  + L+VNIGDL QAWSN+ YKSV+H+V+AN+ +ERYS AYFLCPS  + IGSC +
Sbjct: 243 AVKPNKDTLIVNIGDLFQAWSNNEYKSVEHQVMANAIKERYSIAYFLCPSYDAFIGSCSE 302

Query: 310 PSPYRHFTFGEYRKQ 325
           P+ YR FTFGEYR Q
Sbjct: 303 PTIYRKFTFGEYRXQ 317

[Close]
BLAST of CmaCh04G000330 vs. TrEMBL
Match: W9SYI1_9ROSA (Gibberellin 2-beta-dioxygenase 8 OS=Morus notabilis GN=L484_014953 PE=3 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 2.3e-115
Identity = 203/317 (64.04%), Postives = 243/317 (76.66%), Query Frame = 1 

Query: 8   VMNSNPPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESGKEKERMAC 67
           +++SNPPL   Y  LL  ++ LQ  +          EECQLP++DL GL+S +  ER+ C
Sbjct: 2   IIDSNPPLLDHYKALLDHSSTLQTAECDGHFDGVAAEECQLPMIDLNGLKSDEALERVEC 61

Query: 68  SSEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAAT 127
           +  I +A++EWGFFQV+NHGI  EL+ +M KEQ KLF  PFE K+TS +L+NSYRWG  T
Sbjct: 62  AKAISKAASEWGFFQVVNHGISLELVKKMRKEQTKLFEAPFEDKATSRLLNNSYRWGTPT 121

Query: 128 ATQPNQFSWSEAFHIPLTKVSEADCYGNFIHLREVMEELATAMSKLARSLAGVLVESLGH 187
           AT+P+QFSWSEAFHIPLTKVSE DCYG+FI LRE  EE A AMSKLARSLAGVL E+LGH
Sbjct: 122 ATRPSQFSWSEAFHIPLTKVSEEDCYGDFISLREPTEEFAAAMSKLARSLAGVLAENLGH 181

Query: 188 RKEVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGGLQLMKGSQ 247
           R++VFE++CDESTCFLRLN YP C    ++ GLVPHTDS +LTIL QD  GGLQLMK  +
Sbjct: 182 RRQVFEEICDESTCFLRLNRYPACAVSSEIFGLVPHTDSGFLTILRQDHVGGLQLMKDYK 241

Query: 248 WVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVANSARERYSTAYFLCPSDHSGIGSC 307
           WV+VKP PEAL+VNIGDL QAWSND +KSV+HRV+ N   ERYS AYFLCPS  S IGSC
Sbjct: 242 WVSVKPNPEALIVNIGDLFQAWSNDVFKSVEHRVMTNK-MERYSIAYFLCPSYDSLIGSC 301

Query: 308 RQPSPYRHFTFGEYRKQ 325
           R+PS YR FTF +YR Q
Sbjct: 302 REPSVYRKFTFRQYRSQ 317

[Close]
BLAST of CmaCh04G000330 vs. TAIR10
Match: AT4G21200.1 (AT4G21200.1 gibberellin 2-oxidase 8)

HSP 1 Score: 303.9 bits (777), Expect = 1.4e-82
Identity = 147/287 (51.22%), Postives = 197/287 (68.64%), Query Frame = 1 

Query: 40  SSVLEECQLPLVDLKGLESGKEKERMACSSEIFEASAEWGFFQVINHGIRSELLSRMSKE 99
           ++V+EE +LP++D+  L  G E+ER  C   I  AS EWGFFQVINHGI  ++L +M +E
Sbjct: 34  TAVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQE 93

Query: 100 QMKLFAVPFEKKSTSGILD-NSYRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGNFIH 159
           Q+++F  PF+KKS S      SYRWG  +AT   Q SWSEAFH+P+T +S+     +F  
Sbjct: 94  QIRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFTT 153

Query: 160 LREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGKVT 219
           L   ME+ A+    LA  LA VL E  G     F++ C  +TC+LR+N YP CP P +V 
Sbjct: 154 LSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVY 213

Query: 220 GLVPHTDSDYLTILHQDSGGGLQLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVK 279
           GL+PHTDSD+LTIL+QD  GGLQL+K ++W+AVKP P+AL++NIGDL QAWSN  YKSV+
Sbjct: 214 GLMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVE 273

Query: 280 HRVVANSARERYSTAYFLCPSDHSGIGSCRQPSP-YRHFTFGEYRKQ 325
           HRV+ N   ER+STAYF+CPS +  +  C    P YR+F+F E+R+Q
Sbjct: 274 HRVMTNPKVERFSTAYFMCPS-YDAVIECSSDRPAYRNFSFREFRQQ 316

[Close]
BLAST of CmaCh04G000330 vs. TAIR10
Match: AT1G50960.1 (AT1G50960.1 gibberellin 2-oxidase 7)

HSP 1 Score: 239.6 bits (610), Expect = 3.1e-63
Identity = 129/319 (40.44%), Postives = 185/319 (57.99%), Query Frame = 1 

Query: 9   MNSNPPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESGKEKERMACS 68
           M S PP    +  +  S+      +S    ++      +LP++DL  L SG+E +R  C 
Sbjct: 1   MASQPPFKTNFCSIFGSSFPNSTSESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCV 60

Query: 69  SEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILD---NSYRWGA 128
            ++  A+ EWGFFQ++NHGI  ++   M  E+ KLF  PF  K      D   NSYRWG 
Sbjct: 61  KQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQPFSVKVRERFSDLSKNSYRWGN 120

Query: 129 ATATQPNQFSWSEAFHIPLTKVSEADCYGNFIHLREVMEELATAMSKLARSLAGVLVESL 188
            +AT P Q+S SEAFHI L++VS      N  +LR ++E     ++++A+ +  +L + +
Sbjct: 121 PSATSPAQYSVSEAFHIILSEVSRISDDRN--NLRTIVETYVQEIARVAQMICEILGKQV 180

Query: 189 GHRKEVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGGLQLMKG 248
               E FE++ +    FLRLN Y    F  +V GLVPHTD+ +LTIL QD  GGL+L   
Sbjct: 181 NVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENN 240

Query: 249 SQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVANSARERYSTAYFLCPSDHSGIG 308
            QW++VKP  EAL VNIGD+ QA SN  Y+SV+HRV++ +  ER S A+F+CP   + I 
Sbjct: 241 GQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPANIERMSIAFFVCPYLETEID 300

Query: 309 SCRQPSPYRHFTFGEYRKQ 325
               P  YR F+F EY++Q
Sbjct: 301 CFGYPKKYRRFSFREYKEQ 317

[Close]
BLAST of CmaCh04G000330 vs. TAIR10
Match: AT3G19000.1 (AT3G19000.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 172.2 bits (435), Expect = 6.2e-43
Identity = 104/299 (34.78%), Postives = 152/299 (50.84%), Query Frame = 1 

Query: 47  QLPLVDLKGLESGKEKERMACSSEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAV 106
           ++P +DL  LE     ++ A + EI EA   WGFFQVINHG+ S L  R+ K   + F +
Sbjct: 31  EIPTIDLSSLED-THHDKTAIAKEIAEACKRWGFFQVINHGLPSALRHRVEKTAAEFFNL 90

Query: 107 PFEKK--------STSGILDNSYRWGAATATQPNQFSWSEAFHIPLTKVSEA-------- 166
             E+K        +  G  D  +        +   F   ++  +P +   E         
Sbjct: 91  TTEEKRKVKRDEVNPMGYHDEEHTKNVRDWKEIFDFFLQDSTIVPASPEPEDTELRKLTN 150

Query: 167 DCYGNFIHLREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPI 226
               N  H REV +E A  + KLA  L  ++  SLG   +      +E T FLR NHYP 
Sbjct: 151 QWPQNPSHFREVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNEQTSFLRFNHYPP 210

Query: 227 CPFPGKVTGLVPHTDSDYLTILHQDSGGGLQLMKGS--QWVAVKPIPEALVVNIGDLLQA 286
           CP P    G+  H D   LT+L QDS GGLQ+ + S  QW+ VKPI +AL++N+G+ +Q 
Sbjct: 211 CPNPELALGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIINMGNCIQV 270

Query: 287 WSNDTYKSVKHRVVANSARERYSTAYFLCPSDHSGIG------SCRQPSPYRHFTFGEY 322
           W+ND Y S +HRVV N+++ER+S  +F  PS  + I       S   P  Y+ + +G++
Sbjct: 271 WTNDEYWSAEHRVVVNTSKERFSIPFFFFPSHEANIEPLEELISEENPPCYKKYNWGKF 328

[Close]
BLAST of CmaCh04G000330 vs. TAIR10
Match: AT5G05600.1 (AT5G05600.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 166.8 bits (421), Expect = 2.6e-41
Identity = 105/310 (33.87%), Postives = 160/310 (51.61%), Query Frame = 1 

Query: 27  AHLQPLQSGYQPTSSVLEECQLPLVDLKGLESGKEKERMACSSEIFEASAEWGFFQVINH 86
           A L+P  +   PT++      +P++DL+GL S +        + I EA   WGFFQV+NH
Sbjct: 46  ASLRPTTTEDAPTAT-----NIPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNH 105

Query: 87  GIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYR-WGAATATQPN-QFSWSEAFHIPL 146
           G++ EL+    +   + F +P   K T      +Y  +G+    +      WS+ + + L
Sbjct: 106 GVKPELMDAARENWREFFHMPVNAKETYSNSPRTYEGYGSRLGVEKGASLDWSDYYFLHL 165

Query: 147 TK--VSEADCYGNFIH-LREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMC--DES 206
               + + + + +F   +REV++E    + KL+  +  VL  +LG +++ F++    +  
Sbjct: 166 LPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENI 225

Query: 207 TCFLRLNHYPICPFPGKVTGLVPHTDSDYLTIL-HQDSGGGLQLMKGSQWVAVKPIPEAL 266
              LR+N+YP CP P    GL PH+D   +TIL   D   GLQ+ K   W+ VKP P A 
Sbjct: 226 GACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAF 285

Query: 267 VVNIGDLLQAWSNDTYKSVKHRVVANSARERYSTAYFLCPSDHSGIG------SCRQPSP 323
           +VNIGD +Q  SN TYKSV+HRV+ NS +ER S A+F  P     I       S   P  
Sbjct: 286 IVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAFFYNPKSDIPIQPLQELVSTHNPPL 345

[Close]
BLAST of CmaCh04G000330 vs. TAIR10
Match: AT5G07200.1 (AT5G07200.1 gibberellin 20-oxidase 3)

HSP 1 Score: 158.3 bits (399), Expect = 9.2e-39
Identity = 106/319 (33.23%), Postives = 156/319 (48.90%), Query Frame = 1 

Query: 3   KEDTTVMNSNPPLFHQYGLLLQSTAHL--QPLQSGYQPTSSVLEECQLPLVDLKGLESGK 62
           KED+++ ++         LL Q + H+  Q +   ++  S+ ++  Q+PL+DL G  SG 
Sbjct: 19  KEDSSIFDAK--------LLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGD 78

Query: 63  EKERMACSSEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNS 122
                  +  + +A+ + GFF + NHG+   LLSR        F  P  +K  +      
Sbjct: 79  SCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACEKQKA-----Q 138

Query: 123 YRWGAATA-----------------TQPNQFSWSEAFHIPLTK--VSE--ADCYGNFIHL 182
            +WG ++                  T   +FS  E  H    K  VS+   D Y +F   
Sbjct: 139 RKWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIHSQTVKDFVSKKMGDGYEDF--- 198

Query: 183 REVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGKVTG 242
            +V +E A AM+ L+  +  +L  SLG  +  F++  ++S    RLN+YP C  P    G
Sbjct: 199 GKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELALG 258

Query: 243 LVPHTDSDYLTILHQDSGGGLQLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKH 299
             PH D   LTILHQD  GGLQ+   ++W ++ P P A VVNIGD   A +N  YKS  H
Sbjct: 259 TGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYKSCLH 318

[Close]
BLAST of CmaCh04G000330 vs. NCBI nr
Match: gi|778698231|ref|XP_004142323.2| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus])

HSP 1 Score: 558.5 bits (1438), Expect = 8.7e-156
Identity = 265/325 (81.54%), Postives = 293/325 (90.15%), Query Frame = 1 

Query: 1   MFKEDTTVM-NSNPPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESG 60
           MFKE+TT+M +SNPPLF QY  LLQSTAHLQP  +G  PT+SV+EECQLPL+DLKGL+S 
Sbjct: 1   MFKENTTMMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSS 60

Query: 61  KEKERMACSSEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDN 120
            EKER+AC  EIFEAS EWGFFQVINHGI +ELL+RM+KEQ+KLF VPFEKK TSGILDN
Sbjct: 61  DEKERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDN 120

Query: 121 SYRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGNFIHLREVMEELATAMSKLARSLAG 180
           SYRWG  TAT PNQFSWSEAFHIPLTKVSEA CYG+FI+LREVMEE+A+AMSK+AR LAG
Sbjct: 121 SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAG 180

Query: 181 VLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGG 240
           VLVES+G RKE+ ED+CDESTCFLRLNHYPICPF G+V+GLVPHTDSD+LTILHQDSGGG
Sbjct: 181 VLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGG 240

Query: 241 LQLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVANSARERYSTAYFLCPS 300
           LQ+MKGSQW+AVKP P+ALVVNIGDLLQAWSNDTYKS KHRVVAN  RERYS AYFLCPS
Sbjct: 241 LQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHRVVANPMRERYSIAYFLCPS 300

Query: 301 DHSGIGSCRQPSPYRHFTFGEYRKQ 325
            HS IGSCR+PSPYRHFTF EYRKQ
Sbjct: 301 YHSQIGSCREPSPYRHFTFAEYRKQ 325

[Close]
BLAST of CmaCh04G000330 vs. NCBI nr
Match: gi|659109410|ref|XP_008454705.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X1 [Cucumis melo])

HSP 1 Score: 446.4 bits (1147), Expect = 4.8e-122
Identity = 211/262 (80.53%), Postives = 237/262 (90.46%), Query Frame = 1 

Query: 6   TTVMNSNPPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESGKEKERM 65
           TT+M+SNPPLF QY  LLQSTAHLQP  +G  PT+SV+EECQLPL+DLKGL+S  EKER+
Sbjct: 48  TTMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSNEKERV 107

Query: 66  ACSSEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGA 125
           AC  EIFEAS EWGFFQVINHGI +ELL+RM+KEQ+KLF VPFEKK TSGILDNSYRWG 
Sbjct: 108 ACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKITSGILDNSYRWGT 167

Query: 126 ATATQPNQFSWSEAFHIPLTKVSEADCYGNFIHLREVMEELATAMSKLARSLAGVLVESL 185
            TAT PNQ SWSEAFHIPLTKVSEA CYG+FI+LREVMEE+A+AMSK+AR LAGVLVES+
Sbjct: 168 PTATHPNQLSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLVESM 227

Query: 186 GHRKEVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGGLQLMKG 245
           G RKE+ ED+CDESTCFLRLNHYPICPF G+V+GLVPHTDSD+LTILHQDSGGGLQ+MKG
Sbjct: 228 GQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKG 287

Query: 246 SQWVAVKPIPEALVVNIGDLLQ 268
           SQW+AVKP P+ALVVNIGDLLQ
Sbjct: 288 SQWLAVKPNPQALVVNIGDLLQ 309

[Close]
BLAST of CmaCh04G000330 vs. NCBI nr
Match: gi|659109414|ref|XP_008454707.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X2 [Cucumis melo])

HSP 1 Score: 446.4 bits (1147), Expect = 4.8e-122
Identity = 211/262 (80.53%), Postives = 237/262 (90.46%), Query Frame = 1 

Query: 6   TTVMNSNPPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESGKEKERM 65
           TT+M+SNPPLF QY  LLQSTAHLQP  +G  PT+SV+EECQLPL+DLKGL+S  EKER+
Sbjct: 7   TTMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSNEKERV 66

Query: 66  ACSSEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGA 125
           AC  EIFEAS EWGFFQVINHGI +ELL+RM+KEQ+KLF VPFEKK TSGILDNSYRWG 
Sbjct: 67  ACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKITSGILDNSYRWGT 126

Query: 126 ATATQPNQFSWSEAFHIPLTKVSEADCYGNFIHLREVMEELATAMSKLARSLAGVLVESL 185
            TAT PNQ SWSEAFHIPLTKVSEA CYG+FI+LREVMEE+A+AMSK+AR LAGVLVES+
Sbjct: 127 PTATHPNQLSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLVESM 186

Query: 186 GHRKEVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGGLQLMKG 245
           G RKE+ ED+CDESTCFLRLNHYPICPF G+V+GLVPHTDSD+LTILHQDSGGGLQ+MKG
Sbjct: 187 GQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKG 246

Query: 246 SQWVAVKPIPEALVVNIGDLLQ 268
           SQW+AVKP P+ALVVNIGDLLQ
Sbjct: 247 SQWLAVKPNPQALVVNIGDLLQ 268

[Close]
BLAST of CmaCh04G000330 vs. NCBI nr
Match: gi|297738275|emb|CBI27476.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 428.7 bits (1101), Expect = 1.0e-116
Identity = 205/318 (64.47%), Postives = 245/318 (77.04%), Query Frame = 1 

Query: 7   TVMNSNPPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESGKEKERMA 66
           T+ NSNPPL   YG+L Q        Q       + +EECQLPL+DL+GL S  E+ER+A
Sbjct: 50  TMTNSNPPLLQHYGVLCQHGETPAVQQRDCGNNGAAMEECQLPLIDLEGLWSESEEERLA 109

Query: 67  CSSEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAA 126
           C+S I  AS++WGFFQV+NHGIR ELLS M +EQ+KLF  PFE+K+   +LDNSYRWG  
Sbjct: 110 CASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWGTP 169

Query: 127 TATQPNQFSWSEAFHIPLTKVSEADCYGNFIHLREVMEELATAMSKLARSLAGVLVESLG 186
           TAT P + SWSEAFHIPLTKVSE  CYG F  LREVM+E A AMS LAR LAGVL ESLG
Sbjct: 170 TATCPKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAESLG 229

Query: 187 HRKEVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGGLQLMKGS 246
           H+K VF+++CDE+TCFLRLN YP CP   +V GLVPHTDSD+LTIL+QD  GGLQLMK S
Sbjct: 230 HQKGVFDEICDENTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMKDS 289

Query: 247 QWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVANSARERYSTAYFLCPSDHSGIGS 306
           +WVAVKP  + L+VNIGDL QAWSN+ YKSV+H+V+AN+ +ERYS AYFLCPS  + IGS
Sbjct: 290 KWVAVKPNKDTLIVNIGDLFQAWSNNEYKSVEHQVMANAIKERYSIAYFLCPSYDAFIGS 349

Query: 307 CRQPSPYRHFTFGEYRKQ 325
           C +P+ YR FTFGEYR+Q
Sbjct: 350 CSEPTIYRKFTFGEYRQQ 367

[Close]
BLAST of CmaCh04G000330 vs. NCBI nr
Match: gi|255555559|ref|XP_002518816.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8 [Ricinus communis])

HSP 1 Score: 427.9 bits (1099), Expect = 1.8e-116
Identity = 210/317 (66.25%), Postives = 244/317 (76.97%), Query Frame = 1 

Query: 8   VMNSNPPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESGKEKERMAC 67
           ++ SNPPL   Y  L       +PL+  + P S  +EECQLPLVDL  L+SG EKER AC
Sbjct: 1   MVESNPPLQLSYTALTHQIP--EPLER-HTPQSG-MEECQLPLVDLNCLKSGAEKERSAC 60

Query: 68  SSEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAAT 127
           SS I  AS+EWGFFQV+NHGI  ELL  M +EQ+KLF  PF +K+T G+L+NSYRWG  T
Sbjct: 61  SSAISTASSEWGFFQVVNHGISPELLKNMRREQVKLFQTPFHRKATCGLLNNSYRWGTPT 120

Query: 128 ATQPNQFSWSEAFHIPLTKVSEADCYGNFIHLREVMEELATAMSKLARSLAGVLVESLGH 187
           AT PNQFSWSEAFHIPLTK+SE  CYG F  LREVM E A AMSKLA+ LAG+L E+LGH
Sbjct: 121 ATCPNQFSWSEAFHIPLTKISEQACYGEFTSLREVMMEFAAAMSKLAKLLAGILAENLGH 180

Query: 188 RKEVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGGLQLMKGSQ 247
            + VFE++C ES CFLRLN YP CP   ++ GLVPHTDSD+LTIL+QD  GGLQLMK S+
Sbjct: 181 PRAVFEEICHESNCFLRLNRYPSCPISPEMFGLVPHTDSDFLTILYQDEVGGLQLMKDSK 240

Query: 248 WVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVANSARERYSTAYFLCPSDHSGIGSC 307
           WVAVKP PEAL+VNIGDL QAWSND YKSV+H+V+AN   ERYS AYFLCPS  S IGSC
Sbjct: 241 WVAVKPNPEALIVNIGDLFQAWSNDIYKSVEHKVMANGKMERYSVAYFLCPSYDSLIGSC 300

Query: 308 RQPSPYRHFTFGEYRKQ 325
           ++PS YR FTFGEYR+Q
Sbjct: 301 KEPSIYREFTFGEYREQ 313

The following BLAST results are available for this feature:
BLAST of CmaCh04G000330 vs. Swiss-Prot
Match NameE-valueIdentityDescription
G2OX8_ARATH2.4e-8151.22Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2[more]
G2OX7_ARATH5.6e-6240.44Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1[more]
GAOX3_ARATH1.6e-3733.23Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana GN=GA20OX3 PE=2 SV=1[more]
GAOX1_ARATH2.8e-3735.00Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2[more]
GAO1B_WHEAT6.9e-3634.67Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1[more]
BLAST of CmaCh04G000330 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KIW0_CUCSA6.0e-15681.54Uncharacterized protein OS=Cucumis sativus GN=Csa_5G052540 PE=3 SV=1[more]
F6HG07_VITVI7.2e-11764.47Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g01650 PE=3 SV=... [more]
B9RY49_RICCO1.2e-11666.25Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_0814470 PE=3 SV=1[more]
A5BF50_VITVI1.0e-11564.76Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_025459 PE=3 SV=1[more]
W9SYI1_9ROSA2.3e-11564.04Gibberellin 2-beta-dioxygenase 8 OS=Morus notabilis GN=L484_014953 PE=3 SV=1[more]
BLAST of CmaCh04G000330 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G21200.11.4e-8251.22 gibberellin 2-oxidase 8[more]
AT1G50960.13.1e-6340.44 gibberellin 2-oxidase 7[more]
AT3G19000.16.2e-4334.78 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT5G05600.12.6e-4133.87 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT5G07200.19.2e-3933.23 gibberellin 20-oxidase 3[more]
BLAST of CmaCh04G000330 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778698231|ref|XP_004142323.2|8.7e-15681.54PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus][more]
gi|659109410|ref|XP_008454705.1|4.8e-12280.53PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X1 [Cucumis melo][more]
gi|659109414|ref|XP_008454707.1|4.8e-12280.53PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X2 [Cucumis melo][more]
gi|297738275|emb|CBI27476.3|1.0e-11664.47unnamed protein product [Vitis vinifera][more]
gi|255555559|ref|XP_002518816.1|1.8e-11666.25PREDICTED: gibberellin 2-beta-dioxygenase 8 [Ricinus communis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR026992DIOX_N
IPR027443IPNS-like
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0016491 oxidoreductase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G000330.1CmaCh04G000330.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 202..298
score: 3.2
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 197..298
score: 14
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 48..143
score: 1.4
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 37..324
score: 2.3
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 38..324
score: 5.2E
NoneNo IPR availablePANTHERPTHR10209:SF177SUBFAMILY NOT NAMEDcoord: 38..324
score: 5.2E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 33..324
score: 2.4

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh04G000330CSPI05G01270Wild cucumber (PI 183967)cmacpiB752
CmaCh04G000330CmoCh04G000320Cucurbita moschata (Rifu)cmacmoB728
CmaCh04G000330Cp4.1LG01g06230Cucurbita pepo (Zucchini)cmacpeB720
CmaCh04G000330Bhi07G000219Wax gourdcmawgoB0890
CmaCh04G000330Carg21988Silver-seed gourdcarcmaB0242
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh04G000330Cucurbita pepo (Zucchini)cmacpeB722
CmaCh04G000330Bottle gourd (USVL1VR-Ls)cmalsiB680
CmaCh04G000330Cucumber (Gy14) v2cgybcmaB669
CmaCh04G000330Cucumber (Gy14) v2cgybcmaB664
CmaCh04G000330Melon (DHL92) v3.6.1cmamedB746
CmaCh04G000330Silver-seed gourdcarcmaB0949
CmaCh04G000330Cucumber (Chinese Long) v3cmacucB0876
CmaCh04G000330Cucumber (Chinese Long) v3cmacucB0879
CmaCh04G000330Watermelon (97103) v2cmawmbB781
CmaCh04G000330Cucurbita maxima (Rimu)cmacmaB531
CmaCh04G000330Cucurbita moschata (Rifu)cmacmoB730
CmaCh04G000330Cucumber (Chinese Long) v2cmacuB743
CmaCh04G000330Melon (DHL92) v3.5.1cmameB659
CmaCh04G000330Watermelon (Charleston Gray)cmawcgB674