Cp4.1LG01g06230 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG01g06230
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionGibberellin 2-oxidase
LocationCp4.1LG01 : 203064 .. 205254 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AGTCACCAATTATGCACAAAGGAAAGAACCAGCAGAGCATAAACAATTACTTAAAAGAAACTAAATAAACAATCAGAGAAAAATAGAACATTCTATTTCATATGGGCCTACTGCCAATTGGGGACAAACTTGCTAGACGACAACCGAAGGGCCCCCATGCAAACTTTTCTTGTCCCCCAAAAGTCAAAAAGTTGAAGGCAGTTTTGGATGTTTCCCTTGCATCGTTTTCCACTTTATAGAGGCTTGGTCACCCATCGCTATAATATTAAAAGAAATTGAGAACGACCTTGCCTTCATAAATATGGAAGAACATGGAAGGAGAGAGAAACAATATTTTCGCGACAATTGTCACTACATGGTAGCCCCTTAACAATGTCCTCTTCTCTTCTCTTTGTTTTCTTCCACTTGTGCTCTTCCTCCTCCCCACAATTTAACAACTTTTTCTTTCTTTCCAAAAACAGAGAATATAAACAGCACTTCTCAGTTTAATAACTTTTGTAGGAAGCCATATGTTTAGGGAAGATACCACAATGATGAACTCCAATCCACCACTCTTCCAACAATATGAGTTGCTTTTGCAATCCACAGCTCATTTACAGCCACTTCACAATGGCTATCAACCAACTAGCTCTGTCCTGGAGGAATGTCAGCTCCCTCTTGTCGATCTTAAAGGTTTGGAAAGTGGCAAAGAAAAAGAGAGGATGGCTTGCAGTAGAGAGATTTTTGAAGCGTCTGCAGAATGGGGATTTTTCCAAGTGATTAACCATGGGATACGCTCAGAGCTGCTGAGCAGAATGAGCAAAGAACAAATGAAGTTGTTTGCGGTGCCCTTTGAGAACAAATCTACAAGTGGGATTTTGGATAATTCTTACAGATGGGGAGCAGCCACTGCAACCCAACCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGACTGCTATGGCGACTTCATCCACTTAAGGTATAGATTTTATCACCCTTCTTCCAACTAGAAACTTCTCTTGAGCATTGTGTCAAATTTATTTAGTTCATATGCTTAAAACACAGTCTATTTAGCCTTTGAACTTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAANTTCACTCGAGATTATGTTTTTCTTTGTTAACATCAGAATGCTTAATATGACAGGCTGTTGGCCTGTCCAGTTGTTTGCTATAATATCGCACCAACTCAGCTAAGAGTCATATAAAAACATGTCACGATAGCATTCCTTGATAGTTTCAATGACAATCACTAAATAAAAGCATAATTCAAAATTTAACAATATAATAAAACAAATCCAAAACCCAACGTCTAAGGACTATAGACTTGTATCTTTAACATTGAAATATTCATGCACTTGAAGATTTTAGGTCTTCTAGACATTTTCACAAACAGTTCGACAGCAAAAGTAATGCATTTCGATATCACAATCAACCAATGTACTAGATTACAAAAACGAAGAGAGTGCATTGCTCACTGACCTTACATCCTCGAACGCCAGGGAAGTGATGGAGGAACTGGCAACCGCCATGTCGAAGCTCGCAAGATCTCTAGCAGGAGTTTTAGTGGAGAGTTTGGGCCACCGGAAGGAAGTATTTGAAGATATGTGCGATGAAAGCACATGCTTCCTCCGATTGAATCACTACCCAATCTGTCCGTTTCCCGGCGAAGTTACCGGCCTGGTGCCCCACACCGATAGCGATTATCTGACCATACTTCATCAAGATGCCGGCGGCGGGCTTGAGCTGATGAAAGGATCGCAATGGGTGGCCGTGAAACCCATTCCGGAAGCCCTTGTCGTCAACATTGGAGATCTTCTACAGGTACAAACTGAAACTCTTTCGAAGTATAATGGTTTGGTAGAAGAGCAAGTAGAAAGTGGGTATAAAAATATAGTTTGAAATGCAGGCTTGGAGCAACGATACGTACAAGAGTGTGAAGCATAGGGTGGTGACTAACTCGGTGCGGGAGAGATATTCGACAGCATACTTCCTCTGCCCATCTAACCATTCTGGAATAGGGAGTTGCCGGCAGCCTTCACCATACAGACACTTCACTTTTGGAGAGTACAGGAAGCAAGTGAGAGATGATGTGGAAAAAACTGGGTACAAAGTAGGGCTTTCCAGATTTCGGCTGTAA

mRNA sequence

AGTCACCAATTATGCACAAAGGAAAGAACCAGCAGAGCATAAACAATTACTTAAAAGAAACTAAATAAACAATCAGAGAAAAATAGAACATTCTATTTCATATGGGCCTACTGCCAATTGGGGACAAACTTGCTAGACGACAACCGAAGGGCCCCCATGCAAACTTTTCTTGTCCCCCAAAAGTCAAAAAGTTGAAGGCAGTTTTGGATGTTTCCCTTGCATCGTTTTCCACTTTATAGAGGCTTGGTCACCCATCGCTATAATATTAAAAGAAATTGAGAACGACCTTGCCTTCATAAATATGGAAGAACATGGAAGGAGAGAGAAACAATATTTTCGCGACAATTGTCACTACATGGTAGCCCCTTAACAATGTCCTCTTCTCTTCTCTTTGTTTTCTTCCACTTGTGCTCTTCCTCCTCCCCACAATTTAACAACTTTTTCTTTCTTTCCAAAAACAGAGAATATAAACAGCACTTCTCAGTTTAATAACTTTTGTAGGAAGCCATATGTTTAGGGAAGATACCACAATGATGAACTCCAATCCACCACTCTTCCAACAATATGAGTTGCTTTTGCAATCCACAGCTCATTTACAGCCACTTCACAATGGCTATCAACCAACTAGCTCTGTCCTGGAGGAATGTCAGCTCCCTCTTGTCGATCTTAAAGGTTTGGAAAGTGGCAAAGAAAAAGAGAGGATGGCTTGCAGTAGAGAGATTTTTGAAGCGTCTGCAGAATGGGGATTTTTCCAAGTGATTAACCATGGGATACGCTCAGAGCTGCTGAGCAGAATGAGCAAAGAACAAATGAAGTTGTTTGCGGTGCCCTTTGAGAACAAATCTACAAGTGGGATTTTGGATAATTCTTACAGATGGGGAGCAGCCACTGCAACCCAACCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGACTGCTATGGCGACTTCATCCACTTAAGGGAAGTGATGGAGGAACTGGCAACCGCCATGTCGAAGCTCGCAAGATCTCTAGCAGGAGTTTTAGTGGAGAGTTTGGGCCACCGGAAGGAAGTATTTGAAGATATGTGCGATGAAAGCACATGCTTCCTCCGATTGAATCACTACCCAATCTGTCCGTTTCCCGGCGAAGTTACCGGCCTGGTGCCCCACACCGATAGCGATTATCTGACCATACTTCATCAAGATGCCGGCGGCGGGCTTGAGCTGATGAAAGGATCGCAATGGGTGGCCGTGAAACCCATTCCGGAAGCCCTTGTCGTCAACATTGGAGATCTTCTACAGGCTTGGAGCAACGATACGTACAAGAGTGTGAAGCATAGGGTGGTGACTAACTCGGTGCGGGAGAGATATTCGACAGCATACTTCCTCTGCCCATCTAACCATTCTGGAATAGGGAGTTGCCGGCAGCCTTCACCATACAGACACTTCACTTTTGGAGAGTACAGGAAGCAAGTGAGAGATGATGTGGAAAAAACTGGGTACAAAGTAGGGCTTTCCAGATTTCGGCTGTAA

Coding sequence (CDS)

ATGTTTAGGGAAGATACCACAATGATGAACTCCAATCCACCACTCTTCCAACAATATGAGTTGCTTTTGCAATCCACAGCTCATTTACAGCCACTTCACAATGGCTATCAACCAACTAGCTCTGTCCTGGAGGAATGTCAGCTCCCTCTTGTCGATCTTAAAGGTTTGGAAAGTGGCAAAGAAAAAGAGAGGATGGCTTGCAGTAGAGAGATTTTTGAAGCGTCTGCAGAATGGGGATTTTTCCAAGTGATTAACCATGGGATACGCTCAGAGCTGCTGAGCAGAATGAGCAAAGAACAAATGAAGTTGTTTGCGGTGCCCTTTGAGAACAAATCTACAAGTGGGATTTTGGATAATTCTTACAGATGGGGAGCAGCCACTGCAACCCAACCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGACTGCTATGGCGACTTCATCCACTTAAGGGAAGTGATGGAGGAACTGGCAACCGCCATGTCGAAGCTCGCAAGATCTCTAGCAGGAGTTTTAGTGGAGAGTTTGGGCCACCGGAAGGAAGTATTTGAAGATATGTGCGATGAAAGCACATGCTTCCTCCGATTGAATCACTACCCAATCTGTCCGTTTCCCGGCGAAGTTACCGGCCTGGTGCCCCACACCGATAGCGATTATCTGACCATACTTCATCAAGATGCCGGCGGCGGGCTTGAGCTGATGAAAGGATCGCAATGGGTGGCCGTGAAACCCATTCCGGAAGCCCTTGTCGTCAACATTGGAGATCTTCTACAGGCTTGGAGCAACGATACGTACAAGAGTGTGAAGCATAGGGTGGTGACTAACTCGGTGCGGGAGAGATATTCGACAGCATACTTCCTCTGCCCATCTAACCATTCTGGAATAGGGAGTTGCCGGCAGCCTTCACCATACAGACACTTCACTTTTGGAGAGTACAGGAAGCAAGTGAGAGATGATGTGGAAAAAACTGGGTACAAAGTAGGGCTTTCCAGATTTCGGCTGTAA

Protein sequence

MFREDTTMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFENKSTSGILDNSYRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDAGGGLELMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL
Homology
[Close]
BLAST of Cp4.1LG01g06230 vs. Swiss-Prot
Match: G2OX8_ARATH (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 329.3 bits (843), Expect = 4.9e-89
Identity = 158/303 (52.15%), Postives = 210/303 (69.31%), Query Frame = 1 

Query: 40  SSVLEECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKE 99
           ++V+EE +LP++D+  L  G E+ER  C   I  AS EWGFFQVINHGI  ++L +M +E
Sbjct: 34  TAVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQE 93

Query: 100 QMKLFAVPFENKSTSGILD-NSYRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGDFIH 159
           Q+++F  PF+ KS S      SYRWG  +AT   Q SWSEAFH+P+T +S+     DF  
Sbjct: 94  QIRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFTT 153

Query: 160 LREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVT 219
           L   ME+ A+    LA  LA VL E  G     F++ C  +TC+LR+N YP CP P EV 
Sbjct: 154 LSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVY 213

Query: 220 GLVPHTDSDYLTILHQDAGGGLELMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVK 279
           GL+PHTDSD+LTIL+QD  GGL+L+K ++W+AVKP P+AL++NIGDL QAWSN  YKSV+
Sbjct: 214 GLMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVE 273

Query: 280 HRVVTNSVRERYSTAYFLCPSNHSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGL 339
           HRV+TN   ER+STAYF+CPS  + I        YR+F+F E+R+QV++DV+K G+KVGL
Sbjct: 274 HRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGL 333

Query: 340 SRF 342
            RF
Sbjct: 334 PRF 333

[Close]
BLAST of Cp4.1LG01g06230 vs. Swiss-Prot
Match: G2OX7_ARATH (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 2.1e-68
Identity = 141/336 (41.96%), Postives = 198/336 (58.93%), Query Frame = 1 

Query: 9   MNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMACS 68
           M S PP    +  +  S+       +    ++      +LP++DL  L SG+E +R  C 
Sbjct: 1   MASQPPFKTNFCSIFGSSFPNSTSESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCV 60

Query: 69  REIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFENKSTSGILD---NSYRWGA 128
           +++  A+ EWGFFQ++NHGI  ++   M  E+ KLF  PF  K      D   NSYRWG 
Sbjct: 61  KQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQPFSVKVRERFSDLSKNSYRWGN 120

Query: 129 ATATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESL 188
            +AT P Q+S SEAFHI L++VS      D  +LR ++E     ++++A+ +  +L + +
Sbjct: 121 PSATSPAQYSVSEAFHIILSEVSRIS--DDRNNLRTIVETYVQEIARVAQMICEILGKQV 180

Query: 189 GHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDAGGGLELMKG 248
               E FE++ +    FLRLN Y    F  EV GLVPHTD+ +LTIL QD  GGLEL   
Sbjct: 181 NVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENN 240

Query: 249 SQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIG 308
            QW++VKP  EAL VNIGD+ QA SN  Y+SV+HRV++ +  ER S A+F+CP   + I 
Sbjct: 241 GQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPANIERMSIAFFVCPYLETEID 300

Query: 309 SCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRF 342
               P  YR F+F EY++Q   DV++TG KVGLSRF
Sbjct: 301 CFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRF 334

[Close]
BLAST of Cp4.1LG01g06230 vs. Swiss-Prot
Match: GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 159.1 bits (401), Expect = 8.8e-38
Identity = 102/276 (36.96%), Postives = 141/276 (51.09%), Query Frame = 1 

Query: 37  QPTSSVLEECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRM 96
           +P+ +VLE   +PL+DL+ L S      +  SR I EA  + GFF V+NHGI  EL+S  
Sbjct: 51  KPSINVLE-LDVPLIDLQNLLSDPSST-LDASRLISEACKKHGFFLVVNHGISEELISDA 110

Query: 97  SKEQMKLFAVPFENKSTSGILDNSYRWGAATATQPNQFS----WSEAFHIPLT-----KV 156
            +   + F +P   K    +L  S       ++   +FS    W E              
Sbjct: 111 HEYTSRFFDMPLSEKQR--VLRKSGESVGYASSFTGRFSTKLPWKETLSFRFCDDMSRSK 170

Query: 157 SEADCYGD-----FIHLREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCF 216
           S  D + D     F    +V +E   AMS L+  +  +L  SLG +++ F +  +E+   
Sbjct: 171 SVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFREFFEENDSI 230

Query: 217 LRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDAGGGLELMKGSQWVAVKPIPEALVVNI 276
           +RLN+YP C  P    G  PH D   LTILHQD   GL++   +QW +++P P+A VVNI
Sbjct: 231 MRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNPKAFVVNI 290

Query: 277 GDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCP 299
           GD   A SND YKS  HR V NS  ER S A+FLCP
Sbjct: 291 GDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCP 322

[Close]
BLAST of Cp4.1LG01g06230 vs. Swiss-Prot
Match: GAOX3_ARATH (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana GN=GA20OX3 PE=2 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.5e-37
Identity = 109/321 (33.96%), Postives = 159/321 (49.53%), Query Frame = 1 

Query: 1   MFREDTTMMNSNPPLFQQYELLLQSTAHL--QPLHNGYQPTSSVLEECQLPLVDLKGLES 60
           +F E+ T  +S+  +F   +LL Q + H+  Q +   ++  S+ ++  Q+PL+DL G  S
Sbjct: 12  IFSENKTKEDSS--IFDA-KLLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLS 71

Query: 61  GKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFENKSTSGILD 120
           G        +R + +A+ + GFF + NHG+   LLSR        F  P   K  +    
Sbjct: 72  GDSCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACEKQKA---- 131

Query: 121 NSYRWGAATA-----------------TQPNQFSWSEAFHIPLTK--VSE--ADCYGDFI 180
              +WG ++                  T   +FS  E  H    K  VS+   D Y DF 
Sbjct: 132 -QRKWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIHSQTVKDFVSKKMGDGYEDF- 191

Query: 181 HLREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEV 240
              +V +E A AM+ L+  +  +L  SLG  +  F++  ++S    RLN+YP C  P   
Sbjct: 192 --GKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELA 251

Query: 241 TGLVPHTDSDYLTILHQDAGGGLELMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSV 299
            G  PH D   LTILHQD  GGL++   ++W ++ P P A VVNIGD   A +N  YKS 
Sbjct: 252 LGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYKSC 311

[Close]
BLAST of Cp4.1LG01g06230 vs. Swiss-Prot
Match: GAO1B_WHEAT (Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 7.4e-37
Identity = 98/274 (35.77%), Postives = 133/274 (48.54%), Query Frame = 1 

Query: 35  GYQPTSSVLEECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLS 94
           G  PT    EE  +PL+D+ G+ SG  +     +R + EA    GFFQV+NHGI +ELL+
Sbjct: 26  GESPTPDAAEELHVPLIDIGGMLSGDPRATAEVTRLVGEACERHGFFQVVNHGIDAELLA 85

Query: 95  RMSKEQMKLFAVPFENKSTSGILDNSYRWGAATATQ--PNQFSWSEAFHI------PLTK 154
              +     F +P   K  +          A++ T    ++  W E          P   
Sbjct: 86  DAHRCVDAFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKETLSFRSCPSDPALV 145

Query: 155 VSE--ADCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLR 214
           V    A    D   L EV     + MS+L+  +  VL ESLG  +  +    + +   +R
Sbjct: 146 VDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAHYRRFFEGNDSIMR 205

Query: 215 LNHYPICPFPGEVTGLVPHTDSDYLTILHQDAGGGLELMKGSQWVAVKPIPEALVVNIGD 274
           LN+YP C  P E  G  PH D   LTILHQD  GGL++    +W +++P  +A VVNIGD
Sbjct: 206 LNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPRADAFVVNIGD 265

Query: 275 LLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCP 299
              A SN  YKS  HR V NS   R S A+FLCP
Sbjct: 266 TFMALSNGRYKSCLHRAVVNSKVPRKSLAFFLCP 299

[Close]
BLAST of Cp4.1LG01g06230 vs. TrEMBL
Match: A0A0A0KIW0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G052540 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 2.2e-168
Identity = 283/344 (82.27%), Postives = 313/344 (90.99%), Query Frame = 1 

Query: 1   MFREDTT-MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESG 60
           MF+E+TT MM+SNPPLF QYE LLQSTAHLQP +NG  PT+SV+EECQLPL+DLKGL+S 
Sbjct: 1   MFKENTTMMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSS 60

Query: 61  KEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFENKSTSGILDN 120
            EKER+AC REIFEAS EWGFFQVINHGI +ELL+RM+KEQ+KLF VPFE K TSGILDN
Sbjct: 61  DEKERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDN 120

Query: 121 SYRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAG 180
           SYRWG  TAT PNQFSWSEAFHIPLTKVSEA CYGDFI+LREVMEE+A+AMSK+AR LAG
Sbjct: 121 SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAG 180

Query: 181 VLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDAGGG 240
           VLVES+G RKE+ ED+CDESTCFLRLNHYPICPF GEV+GLVPHTDSD+LTILHQD+GGG
Sbjct: 181 VLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGG 240

Query: 241 LELMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPS 300
           L++MKGSQW+AVKP P+ALVVNIGDLLQAWSNDTYKS KHRVV N +RERYS AYFLCPS
Sbjct: 241 LQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHRVVANPMRERYSIAYFLCPS 300

Query: 301 NHSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 344
            HS IGSCR+PSPYRHFTF EYRKQVRDDVEKTGYK+GLS FRL
Sbjct: 301 YHSQIGSCREPSPYRHFTFAEYRKQVRDDVEKTGYKIGLSNFRL 344

[Close]
BLAST of Cp4.1LG01g06230 vs. TrEMBL
Match: W9SYI1_9ROSA (Gibberellin 2-beta-dioxygenase 8 OS=Morus notabilis GN=L484_014953 PE=3 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 2.3e-125
Identity = 219/336 (65.18%), Postives = 264/336 (78.57%), Query Frame = 1 

Query: 8   MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMAC 67
           +++SNPPL   Y+ LL  ++ LQ             EECQLP++DL GL+S +  ER+ C
Sbjct: 2   IIDSNPPLLDHYKALLDHSSTLQTAECDGHFDGVAAEECQLPMIDLNGLKSDEALERVEC 61

Query: 68  SREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFENKSTSGILDNSYRWGAAT 127
           ++ I +A++EWGFFQV+NHGI  EL+ +M KEQ KLF  PFE+K+TS +L+NSYRWG  T
Sbjct: 62  AKAISKAASEWGFFQVVNHGISLELVKKMRKEQTKLFEAPFEDKATSRLLNNSYRWGTPT 121

Query: 128 ATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGH 187
           AT+P+QFSWSEAFHIPLTKVSE DCYGDFI LRE  EE A AMSKLARSLAGVL E+LGH
Sbjct: 122 ATRPSQFSWSEAFHIPLTKVSEEDCYGDFISLREPTEEFAAAMSKLARSLAGVLAENLGH 181

Query: 188 RKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDAGGGLELMKGSQ 247
           R++VFE++CDESTCFLRLN YP C    E+ GLVPHTDS +LTIL QD  GGL+LMK  +
Sbjct: 182 RRQVFEEICDESTCFLRLNRYPACAVSSEIFGLVPHTDSGFLTILRQDHVGGLQLMKDYK 241

Query: 248 WVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIGSC 307
           WV+VKP PEAL+VNIGDL QAWSND +KSV+HRV+TN + ERYS AYFLCPS  S IGSC
Sbjct: 242 WVSVKPNPEALIVNIGDLFQAWSNDVFKSVEHRVMTNKM-ERYSIAYFLCPSYDSLIGSC 301

Query: 308 RQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 344
           R+PS YR FTF +YR QV++DV+K GYKVGLSRF L
Sbjct: 302 REPSVYRKFTFRQYRSQVQEDVKKMGYKVGLSRFLL 336

[Close]
BLAST of Cp4.1LG01g06230 vs. TrEMBL
Match: F6HG07_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g01650 PE=3 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 7.3e-124
Identity = 216/337 (64.09%), Postives = 260/337 (77.15%), Query Frame = 1 

Query: 7   TMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMA 66
           TM NSNPPL Q Y +L Q                + +EECQLPL+DL+GL S  E+ER+A
Sbjct: 23  TMTNSNPPLLQHYGVLCQHGETPAVQQRDCGNNGAAMEECQLPLIDLEGLWSESEEERLA 82

Query: 67  CSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFENKSTSGILDNSYRWGAA 126
           C+  I  AS++WGFFQV+NHGIR ELLS M +EQ+KLF  PFE K+   +LDNSYRWG  
Sbjct: 83  CASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWGTP 142

Query: 127 TATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESLG 186
           TAT P + SWSEAFHIPLTKVSE  CYG+F  LREVM+E A AMS LAR LAGVL ESLG
Sbjct: 143 TATCPKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAESLG 202

Query: 187 HRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDAGGGLELMKGS 246
           H+K VF+++CDE+TCFLRLN YP CP   EV GLVPHTDSD+LTIL+QD  GGL+LMK S
Sbjct: 203 HQKGVFDEICDENTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMKDS 262

Query: 247 QWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIGS 306
           +WVAVKP  + L+VNIGDL QAWSN+ YKSV+H+V+ N+++ERYS AYFLCPS  + IGS
Sbjct: 263 KWVAVKPNKDTLIVNIGDLFQAWSNNEYKSVEHQVMANAIKERYSIAYFLCPSYDAFIGS 322

Query: 307 CRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 344
           C +P+ YR FTFGEYR+QV++DV+KTG+KVGL RF L
Sbjct: 323 CSEPTIYRKFTFGEYRQQVQEDVKKTGHKVGLPRFLL 359

[Close]
BLAST of Cp4.1LG01g06230 vs. TrEMBL
Match: B9RY49_RICCO (Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_0814470 PE=3 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 9.5e-124
Identity = 221/336 (65.77%), Postives = 257/336 (76.49%), Query Frame = 1 

Query: 8   MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMAC 67
           M+ SNPPL   Y  L       +PL   + P S  +EECQLPLVDL  L+SG EKER AC
Sbjct: 1   MVESNPPLQLSYTALTHQIP--EPLER-HTPQSG-MEECQLPLVDLNCLKSGAEKERSAC 60

Query: 68  SREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFENKSTSGILDNSYRWGAAT 127
           S  I  AS+EWGFFQV+NHGI  ELL  M +EQ+KLF  PF  K+T G+L+NSYRWG  T
Sbjct: 61  SSAISTASSEWGFFQVVNHGISPELLKNMRREQVKLFQTPFHRKATCGLLNNSYRWGTPT 120

Query: 128 ATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGH 187
           AT PNQFSWSEAFHIPLTK+SE  CYG+F  LREVM E A AMSKLA+ LAG+L E+LGH
Sbjct: 121 ATCPNQFSWSEAFHIPLTKISEQACYGEFTSLREVMMEFAAAMSKLAKLLAGILAENLGH 180

Query: 188 RKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDAGGGLELMKGSQ 247
            + VFE++C ES CFLRLN YP CP   E+ GLVPHTDSD+LTIL+QD  GGL+LMK S+
Sbjct: 181 PRAVFEEICHESNCFLRLNRYPSCPISPEMFGLVPHTDSDFLTILYQDEVGGLQLMKDSK 240

Query: 248 WVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIGSC 307
           WVAVKP PEAL+VNIGDL QAWSND YKSV+H+V+ N   ERYS AYFLCPS  S IGSC
Sbjct: 241 WVAVKPNPEALIVNIGDLFQAWSNDIYKSVEHKVMANGKMERYSVAYFLCPSYDSLIGSC 300

Query: 308 RQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 344
           ++PS YR FTFGEYR+QVR+DV+ TG+K+GL RF L
Sbjct: 301 KEPSIYREFTFGEYREQVREDVKTTGHKIGLPRFLL 332

[Close]
BLAST of Cp4.1LG01g06230 vs. TrEMBL
Match: A5BF50_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_025459 PE=3 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 8.1e-123
Identity = 215/336 (63.99%), Postives = 258/336 (76.79%), Query Frame = 1 

Query: 8   MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMAC 67
           M NSNPPL Q Y +L Q                + +EECQLPL+DL+GL S  E+ER+AC
Sbjct: 1   MTNSNPPLLQHYGVLCQHGETPAVQQRDCGNNGAAMEECQLPLIDLEGLWSESEEERLAC 60

Query: 68  SREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFENKSTSGILDNSYRWGAAT 127
           +  I  AS++WGFFQV+NHGIR ELLS M +EQ+KLF  PFE K+   +LDNSYRWG  T
Sbjct: 61  ASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWGTPT 120

Query: 128 ATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGH 187
           AT+  + SWSEAFHIPLTKVSE  CYG+F  LREVM+E A AMS LAR LAGVL ESLGH
Sbjct: 121 ATRLKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAESLGH 180

Query: 188 RKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDAGGGLELMKGSQ 247
           +K VF+++CDESTCFLRLN YP CP   EV GLVPHTDSD+LTIL+QD  GGL+LMK S+
Sbjct: 181 QKGVFDEICDESTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMKDSK 240

Query: 248 WVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIGSC 307
           WVAVKP  + L+VNIGDL QAWSN+ YKSV+H+V+ N+++ERYS AYFLCPS  + IGSC
Sbjct: 241 WVAVKPNKDTLIVNIGDLFQAWSNNEYKSVEHQVMANAIKERYSIAYFLCPSYDAFIGSC 300

Query: 308 RQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 344
            +P+ YR FTFGEYR QV++DV+KTG+KVGL RF L
Sbjct: 301 SEPTIYRKFTFGEYRXQVQEDVKKTGHKVGLPRFLL 336

[Close]
BLAST of Cp4.1LG01g06230 vs. TAIR10
Match: AT4G21200.1 (AT4G21200.1 gibberellin 2-oxidase 8)

HSP 1 Score: 329.3 bits (843), Expect = 2.8e-90
Identity = 158/303 (52.15%), Postives = 210/303 (69.31%), Query Frame = 1 

Query: 40  SSVLEECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKE 99
           ++V+EE +LP++D+  L  G E+ER  C   I  AS EWGFFQVINHGI  ++L +M +E
Sbjct: 34  TAVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQE 93

Query: 100 QMKLFAVPFENKSTSGILD-NSYRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGDFIH 159
           Q+++F  PF+ KS S      SYRWG  +AT   Q SWSEAFH+P+T +S+     DF  
Sbjct: 94  QIRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFTT 153

Query: 160 LREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVT 219
           L   ME+ A+    LA  LA VL E  G     F++ C  +TC+LR+N YP CP P EV 
Sbjct: 154 LSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVY 213

Query: 220 GLVPHTDSDYLTILHQDAGGGLELMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVK 279
           GL+PHTDSD+LTIL+QD  GGL+L+K ++W+AVKP P+AL++NIGDL QAWSN  YKSV+
Sbjct: 214 GLMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVE 273

Query: 280 HRVVTNSVRERYSTAYFLCPSNHSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGL 339
           HRV+TN   ER+STAYF+CPS  + I        YR+F+F E+R+QV++DV+K G+KVGL
Sbjct: 274 HRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGL 333

Query: 340 SRF 342
            RF
Sbjct: 334 PRF 333

[Close]
BLAST of Cp4.1LG01g06230 vs. TAIR10
Match: AT1G50960.1 (AT1G50960.1 gibberellin 2-oxidase 7)

HSP 1 Score: 260.8 bits (665), Expect = 1.2e-69
Identity = 141/336 (41.96%), Postives = 198/336 (58.93%), Query Frame = 1 

Query: 9   MNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMACS 68
           M S PP    +  +  S+       +    ++      +LP++DL  L SG+E +R  C 
Sbjct: 1   MASQPPFKTNFCSIFGSSFPNSTSESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCV 60

Query: 69  REIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFENKSTSGILD---NSYRWGA 128
           +++  A+ EWGFFQ++NHGI  ++   M  E+ KLF  PF  K      D   NSYRWG 
Sbjct: 61  KQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQPFSVKVRERFSDLSKNSYRWGN 120

Query: 129 ATATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESL 188
            +AT P Q+S SEAFHI L++VS      D  +LR ++E     ++++A+ +  +L + +
Sbjct: 121 PSATSPAQYSVSEAFHIILSEVSRIS--DDRNNLRTIVETYVQEIARVAQMICEILGKQV 180

Query: 189 GHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDAGGGLELMKG 248
               E FE++ +    FLRLN Y    F  EV GLVPHTD+ +LTIL QD  GGLEL   
Sbjct: 181 NVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENN 240

Query: 249 SQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIG 308
            QW++VKP  EAL VNIGD+ QA SN  Y+SV+HRV++ +  ER S A+F+CP   + I 
Sbjct: 241 GQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPANIERMSIAFFVCPYLETEID 300

Query: 309 SCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRF 342
               P  YR F+F EY++Q   DV++TG KVGLSRF
Sbjct: 301 CFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRF 334

[Close]
BLAST of Cp4.1LG01g06230 vs. TAIR10
Match: AT3G19000.1 (AT3G19000.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 168.3 bits (425), Expect = 8.2e-42
Identity = 101/299 (33.78%), Postives = 151/299 (50.50%), Query Frame = 1 

Query: 47  QLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAV 106
           ++P +DL  LE     ++ A ++EI EA   WGFFQVINHG+ S L  R+ K   + F +
Sbjct: 31  EIPTIDLSSLED-THHDKTAIAKEIAEACKRWGFFQVINHGLPSALRHRVEKTAAEFFNL 90

Query: 107 PFENK--------STSGILDNSYRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGDFI- 166
             E K        +  G  D  +        +   F   ++  +P +   E         
Sbjct: 91  TTEEKRKVKRDEVNPMGYHDEEHTKNVRDWKEIFDFFLQDSTIVPASPEPEDTELRKLTN 150

Query: 167 -------HLREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPI 226
                  H REV +E A  + KLA  L  ++  SLG   +      +E T FLR NHYP 
Sbjct: 151 QWPQNPSHFREVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNEQTSFLRFNHYPP 210

Query: 227 CPFPGEVTGLVPHTDSDYLTILHQDAGGGLELMKGS--QWVAVKPIPEALVVNIGDLLQA 286
           CP P    G+  H D   LT+L QD+ GGL++ + S  QW+ VKPI +AL++N+G+ +Q 
Sbjct: 211 CPNPELALGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIINMGNCIQV 270

Query: 287 WSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIG------SCRQPSPYRHFTFGEY 322
           W+ND Y S +HRVV N+ +ER+S  +F  PS+ + I       S   P  Y+ + +G++
Sbjct: 271 WTNDEYWSAEHRVVVNTSKERFSIPFFFFPSHEANIEPLEELISEENPPCYKKYNWGKF 328

[Close]
BLAST of Cp4.1LG01g06230 vs. TAIR10
Match: AT5G58660.1 (AT5G58660.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 166.8 bits (421), Expect = 2.4e-41
Identity = 111/332 (33.43%), Postives = 166/332 (50.00%), Query Frame = 1 

Query: 44  EECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKL 103
           ++  +P++DL+ L+  KE  R AC         EWG F++ NHG+   L SR+ +    L
Sbjct: 30  KDINIPVIDLERLD--KEILREACK--------EWGIFRLENHGVPLALTSRLQEISESL 89

Query: 104 FAVPFENKSTSGILDNS---YRWGAAT----------ATQPNQFSWSEAFHIPLTKVSE- 163
            ++PFE K        S   Y WG               Q +  +  E F++PL+ +S  
Sbjct: 90  LSLPFEKKRELFAAVKSPLSYFWGTPALNRSGDALKRGAQASNLTMLEGFNVPLSSLSSL 149

Query: 164 -----ADCYGDFIH-------LREVMEELATAMSKLARSLAGVLVESL-----GHRKEVF 223
                + C  D           R +MEE    ++++A SL   + ++L     G+R+  +
Sbjct: 150 SKLPTSTCCDDDAQEEPKLESFRVLMEEYGKHITRIAVSLFEAIAQTLNLELSGNRRSEY 209

Query: 224 EDMCDESTCFLRLNHYPICPFPG--EVTGLVPHTDSDYLTILHQDAGGGLELMKGSQWVA 283
                EST  +R+  YP        E  G+  HTDS  ++IL +D  GGLE+MKG +W  
Sbjct: 210 ---LSESTGLIRVYRYPQSSEEAAREALGMEVHTDSSVISILREDESGGLEIMKGEEWFC 269

Query: 284 VKPIPEALVVNIGDLLQAWSNDTYKSVKHRV-VTNSVRERYSTAYFLCPSNHSGIGSCRQ 342
           VKP+   L+VN+GD++QA S+D YKSV HRV   N   ER+S  YF+ P     I S   
Sbjct: 270 VKPVANTLIVNLGDMMQAISDDEYKSVTHRVKKRNRKTERHSVCYFVFPKRDCVIKS--- 329

[Close]
BLAST of Cp4.1LG01g06230 vs. TAIR10
Match: AT4G25420.1 (AT4G25420.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 159.1 bits (401), Expect = 5.0e-39
Identity = 102/276 (36.96%), Postives = 141/276 (51.09%), Query Frame = 1 

Query: 37  QPTSSVLEECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRM 96
           +P+ +VLE   +PL+DL+ L S      +  SR I EA  + GFF V+NHGI  EL+S  
Sbjct: 51  KPSINVLE-LDVPLIDLQNLLSDPSST-LDASRLISEACKKHGFFLVVNHGISEELISDA 110

Query: 97  SKEQMKLFAVPFENKSTSGILDNSYRWGAATATQPNQFS----WSEAFHIPLT-----KV 156
            +   + F +P   K    +L  S       ++   +FS    W E              
Sbjct: 111 HEYTSRFFDMPLSEKQR--VLRKSGESVGYASSFTGRFSTKLPWKETLSFRFCDDMSRSK 170

Query: 157 SEADCYGD-----FIHLREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCF 216
           S  D + D     F    +V +E   AMS L+  +  +L  SLG +++ F +  +E+   
Sbjct: 171 SVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFREFFEENDSI 230

Query: 217 LRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDAGGGLELMKGSQWVAVKPIPEALVVNI 276
           +RLN+YP C  P    G  PH D   LTILHQD   GL++   +QW +++P P+A VVNI
Sbjct: 231 MRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNPKAFVVNI 290

Query: 277 GDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCP 299
           GD   A SND YKS  HR V NS  ER S A+FLCP
Sbjct: 291 GDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCP 322

[Close]
BLAST of Cp4.1LG01g06230 vs. NCBI nr
Match: gi|778698231|ref|XP_004142323.2| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus])

HSP 1 Score: 599.7 bits (1545), Expect = 3.1e-168
Identity = 283/344 (82.27%), Postives = 313/344 (90.99%), Query Frame = 1 

Query: 1   MFREDTT-MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESG 60
           MF+E+TT MM+SNPPLF QYE LLQSTAHLQP +NG  PT+SV+EECQLPL+DLKGL+S 
Sbjct: 1   MFKENTTMMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSS 60

Query: 61  KEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFENKSTSGILDN 120
            EKER+AC REIFEAS EWGFFQVINHGI +ELL+RM+KEQ+KLF VPFE K TSGILDN
Sbjct: 61  DEKERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDN 120

Query: 121 SYRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAG 180
           SYRWG  TAT PNQFSWSEAFHIPLTKVSEA CYGDFI+LREVMEE+A+AMSK+AR LAG
Sbjct: 121 SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAG 180

Query: 181 VLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDAGGG 240
           VLVES+G RKE+ ED+CDESTCFLRLNHYPICPF GEV+GLVPHTDSD+LTILHQD+GGG
Sbjct: 181 VLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGG 240

Query: 241 LELMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPS 300
           L++MKGSQW+AVKP P+ALVVNIGDLLQAWSNDTYKS KHRVV N +RERYS AYFLCPS
Sbjct: 241 LQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHRVVANPMRERYSIAYFLCPS 300

Query: 301 NHSGIGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 344
            HS IGSCR+PSPYRHFTF EYRKQVRDDVEKTGYK+GLS FRL
Sbjct: 301 YHSQIGSCREPSPYRHFTFAEYRKQVRDDVEKTGYKIGLSNFRL 344

[Close]
BLAST of Cp4.1LG01g06230 vs. NCBI nr
Match: gi|703159524|ref|XP_010112271.1| (Gibberellin 2-beta-dioxygenase 8 [Morus notabilis])

HSP 1 Score: 456.8 bits (1174), Expect = 3.3e-125
Identity = 219/336 (65.18%), Postives = 264/336 (78.57%), Query Frame = 1 

Query: 8   MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMAC 67
           +++SNPPL   Y+ LL  ++ LQ             EECQLP++DL GL+S +  ER+ C
Sbjct: 2   IIDSNPPLLDHYKALLDHSSTLQTAECDGHFDGVAAEECQLPMIDLNGLKSDEALERVEC 61

Query: 68  SREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFENKSTSGILDNSYRWGAAT 127
           ++ I +A++EWGFFQV+NHGI  EL+ +M KEQ KLF  PFE+K+TS +L+NSYRWG  T
Sbjct: 62  AKAISKAASEWGFFQVVNHGISLELVKKMRKEQTKLFEAPFEDKATSRLLNNSYRWGTPT 121

Query: 128 ATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGH 187
           AT+P+QFSWSEAFHIPLTKVSE DCYGDFI LRE  EE A AMSKLARSLAGVL E+LGH
Sbjct: 122 ATRPSQFSWSEAFHIPLTKVSEEDCYGDFISLREPTEEFAAAMSKLARSLAGVLAENLGH 181

Query: 188 RKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDAGGGLELMKGSQ 247
           R++VFE++CDESTCFLRLN YP C    E+ GLVPHTDS +LTIL QD  GGL+LMK  +
Sbjct: 182 RRQVFEEICDESTCFLRLNRYPACAVSSEIFGLVPHTDSGFLTILRQDHVGGLQLMKDYK 241

Query: 248 WVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIGSC 307
           WV+VKP PEAL+VNIGDL QAWSND +KSV+HRV+TN + ERYS AYFLCPS  S IGSC
Sbjct: 242 WVSVKPNPEALIVNIGDLFQAWSNDVFKSVEHRVMTNKM-ERYSIAYFLCPSYDSLIGSC 301

Query: 308 RQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 344
           R+PS YR FTF +YR QV++DV+K GYKVGLSRF L
Sbjct: 302 REPSVYRKFTFRQYRSQVQEDVKKMGYKVGLSRFLL 336

[Close]
BLAST of Cp4.1LG01g06230 vs. NCBI nr
Match: gi|659109410|ref|XP_008454705.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X1 [Cucumis melo])

HSP 1 Score: 454.9 bits (1169), Expect = 1.2e-124
Identity = 214/262 (81.68%), Postives = 239/262 (91.22%), Query Frame = 1 

Query: 6   TTMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERM 65
           TTMM+SNPPLF QYE LLQSTAHLQP +NG  PT+SV+EECQLPL+DLKGL+S  EKER+
Sbjct: 48  TTMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSNEKERV 107

Query: 66  ACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFENKSTSGILDNSYRWGA 125
           AC REIFEAS EWGFFQVINHGI +ELL+RM+KEQ+KLF VPFE K TSGILDNSYRWG 
Sbjct: 108 ACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKITSGILDNSYRWGT 167

Query: 126 ATATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESL 185
            TAT PNQ SWSEAFHIPLTKVSEA CYGDFI+LREVMEE+A+AMSK+AR LAGVLVES+
Sbjct: 168 PTATHPNQLSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLVESM 227

Query: 186 GHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDAGGGLELMKG 245
           G RKE+ ED+CDESTCFLRLNHYPICPF GEV+GLVPHTDSD+LTILHQD+GGGL++MKG
Sbjct: 228 GQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKG 287

Query: 246 SQWVAVKPIPEALVVNIGDLLQ 268
           SQW+AVKP P+ALVVNIGDLLQ
Sbjct: 288 SQWLAVKPNPQALVVNIGDLLQ 309

[Close]
BLAST of Cp4.1LG01g06230 vs. NCBI nr
Match: gi|659109414|ref|XP_008454707.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X2 [Cucumis melo])

HSP 1 Score: 454.9 bits (1169), Expect = 1.2e-124
Identity = 214/262 (81.68%), Postives = 239/262 (91.22%), Query Frame = 1 

Query: 6   TTMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERM 65
           TTMM+SNPPLF QYE LLQSTAHLQP +NG  PT+SV+EECQLPL+DLKGL+S  EKER+
Sbjct: 7   TTMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSNEKERV 66

Query: 66  ACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFENKSTSGILDNSYRWGA 125
           AC REIFEAS EWGFFQVINHGI +ELL+RM+KEQ+KLF VPFE K TSGILDNSYRWG 
Sbjct: 67  ACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKITSGILDNSYRWGT 126

Query: 126 ATATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESL 185
            TAT PNQ SWSEAFHIPLTKVSEA CYGDFI+LREVMEE+A+AMSK+AR LAGVLVES+
Sbjct: 127 PTATHPNQLSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLVESM 186

Query: 186 GHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDAGGGLELMKG 245
           G RKE+ ED+CDESTCFLRLNHYPICPF GEV+GLVPHTDSD+LTILHQD+GGGL++MKG
Sbjct: 187 GQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKG 246

Query: 246 SQWVAVKPIPEALVVNIGDLLQ 268
           SQW+AVKP P+ALVVNIGDLLQ
Sbjct: 247 SQWLAVKPNPQALVVNIGDLLQ 268

[Close]
BLAST of Cp4.1LG01g06230 vs. NCBI nr
Match: gi|297738275|emb|CBI27476.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 451.8 bits (1161), Expect = 1.0e-123
Identity = 216/337 (64.09%), Postives = 260/337 (77.15%), Query Frame = 1 

Query: 7   TMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMA 66
           TM NSNPPL Q Y +L Q                + +EECQLPL+DL+GL S  E+ER+A
Sbjct: 50  TMTNSNPPLLQHYGVLCQHGETPAVQQRDCGNNGAAMEECQLPLIDLEGLWSESEEERLA 109

Query: 67  CSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFENKSTSGILDNSYRWGAA 126
           C+  I  AS++WGFFQV+NHGIR ELLS M +EQ+KLF  PFE K+   +LDNSYRWG  
Sbjct: 110 CASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWGTP 169

Query: 127 TATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESLG 186
           TAT P + SWSEAFHIPLTKVSE  CYG+F  LREVM+E A AMS LAR LAGVL ESLG
Sbjct: 170 TATCPKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAESLG 229

Query: 187 HRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDAGGGLELMKGS 246
           H+K VF+++CDE+TCFLRLN YP CP   EV GLVPHTDSD+LTIL+QD  GGL+LMK S
Sbjct: 230 HQKGVFDEICDENTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMKDS 289

Query: 247 QWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNHSGIGS 306
           +WVAVKP  + L+VNIGDL QAWSN+ YKSV+H+V+ N+++ERYS AYFLCPS  + IGS
Sbjct: 290 KWVAVKPNKDTLIVNIGDLFQAWSNNEYKSVEHQVMANAIKERYSIAYFLCPSYDAFIGS 349

Query: 307 CRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 344
           C +P+ YR FTFGEYR+QV++DV+KTG+KVGL RF L
Sbjct: 350 CSEPTIYRKFTFGEYRQQVQEDVKKTGHKVGLPRFLL 386

The following BLAST results are available for this feature:
BLAST of Cp4.1LG01g06230 vs. Swiss-Prot
Match NameE-valueIdentityDescription
G2OX8_ARATH4.9e-8952.15Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2[more]
G2OX7_ARATH2.1e-6841.96Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1[more]
GAOX1_ARATH8.8e-3836.96Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2[more]
GAOX3_ARATH1.5e-3733.96Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana GN=GA20OX3 PE=2 SV=1[more]
GAO1B_WHEAT7.4e-3735.77Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1[more]
BLAST of Cp4.1LG01g06230 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KIW0_CUCSA2.2e-16882.27Uncharacterized protein OS=Cucumis sativus GN=Csa_5G052540 PE=3 SV=1[more]
W9SYI1_9ROSA2.3e-12565.18Gibberellin 2-beta-dioxygenase 8 OS=Morus notabilis GN=L484_014953 PE=3 SV=1[more]
F6HG07_VITVI7.3e-12464.09Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g01650 PE=3 SV=... [more]
B9RY49_RICCO9.5e-12465.77Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_0814470 PE=3 SV=1[more]
A5BF50_VITVI8.1e-12363.99Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_025459 PE=3 SV=1[more]
BLAST of Cp4.1LG01g06230 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G21200.12.8e-9052.15 gibberellin 2-oxidase 8[more]
AT1G50960.11.2e-6941.96 gibberellin 2-oxidase 7[more]
AT3G19000.18.2e-4233.78 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT5G58660.12.4e-4133.43 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G25420.15.0e-3936.96 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
BLAST of Cp4.1LG01g06230 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778698231|ref|XP_004142323.2|3.1e-16882.27PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus][more]
gi|703159524|ref|XP_010112271.1|3.3e-12565.18Gibberellin 2-beta-dioxygenase 8 [Morus notabilis][more]
gi|659109410|ref|XP_008454705.1|1.2e-12481.68PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X1 [Cucumis melo][more]
gi|659109414|ref|XP_008454707.1|1.2e-12481.68PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X2 [Cucumis melo][more]
gi|297738275|emb|CBI27476.3|1.0e-12364.09unnamed protein product [Vitis vinifera][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: INTERPRO
TermDefinition
IPR027443IPNS-like
IPR026992DIOX_N
IPR005123Oxoglu/Fe-dep_dioxygenase
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0016491 oxidoreductase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g06230.1Cp4.1LG01g06230.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 202..298
score: 1.1
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 197..298
score: 14
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 48..143
score: 1.8
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 37..325
score: 1.1
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 1..336
score: 5.4E
NoneNo IPR availablePANTHERPTHR10209:SF177SUBFAMILY NOT NAMEDcoord: 1..336
score: 5.4E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 33..325
score: 3.57

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG01g06230CmaCh04G000330Cucurbita maxima (Rimu)cmacpeB720
Cp4.1LG01g06230CmoCh04G000320Cucurbita moschata (Rifu)cmocpeB673
Cp4.1LG01g06230Csa5G052540Cucumber (Chinese Long) v2cpecuB457
Cp4.1LG01g06230MELO3C034260.2Melon (DHL92) v3.6.1cpemedB427
Cp4.1LG01g06230Bhi07G000219Wax gourdcpewgoB0554
Cp4.1LG01g06230Carg21988Silver-seed gourdcarcpeB0258
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG01g06230Silver-seed gourdcarcpeB0939
Cp4.1LG01g06230Cucumber (Chinese Long) v3cpecucB0532
Cp4.1LG01g06230Cucurbita pepo (Zucchini)cpecpeB374
Cp4.1LG01g06230Cucurbita maxima (Rimu)cmacpeB723
Cp4.1LG01g06230Cucurbita moschata (Rifu)cmocpeB675
Cp4.1LG01g06230Wild cucumber (PI 183967)cpecpiB434
Cp4.1LG01g06230Wild cucumber (PI 183967)cpecpiB456
Cp4.1LG01g06230Cucumber (Chinese Long) v2cpecuB435
Cp4.1LG01g06230Bottle gourd (USVL1VR-Ls)cpelsiB362
Cp4.1LG01g06230Watermelon (Charleston Gray)cpewcgB410
Cp4.1LG01g06230Melon (DHL92) v3.5.1cpemeB370
Cp4.1LG01g06230Cucumber (Gy14) v2cgybcpeB642