CmoCh04G000320 (gene) Cucurbita moschata (Rifu)

Overview
NameCmoCh04G000320
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationCmo_Chr04 : 209319 .. 210955 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTAAGGAAGATACCACAATGATGAACTCCAATCCACCACTCTTCCAACAATATGAGTTGCTTTTGCAATCCACAGCTCATTTACAGCCACTTCACAATGGCTATCAACCAACTAGCTCTGTCCTGGAGGAATGTCAGCTCCCTCTTGTCGATCTTAAAGGTTTGGAAAGTGGCAAAGAAAAAGAGAGGATGGCTTGCAGTAGAGAGATTTTTGAAGCATCTGCAGAATGGGGATTTTTCCAAGTGATTAACCATGGGATACGCTCAGAGCTGCTGAGCAGAATGAGCAAAGAACAAATGAAGTTGTTTGCGGTGCCCTTTGAGAAAAAATCTACAAGTGGGATTTTGGATAATTCTTACCGATGGGGAGCAGCCACTGCAACCCAACCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGACTGCTATGGCGACTTCATTCACTTAAGGTATAGATTTTATTACCCTTCTTCCAACTAGAAACTTCTCTTGAGCATTGTGTCAAATTTATTTAGTTCATATGCTTAAAACACAGTCTATTTAGCCTTTGAACTTACACACAAAAAAAAAAAAAAAAAAAACCCCCATTTCATTCTAGGTTATGTTTTTCTTTGTTAACATCAGAATGCTTAATATGACAGGCTGTTCGCCTGTCCAGTTGTTTGCTATAATATCGCACCAACTCAGCTAAGAGTCATATAAAAACATGTCACGATAGCATTCCTTGATAGTTTCAATGACAATCACTAAATAAAAGCATAATTCAAAATTTAACAATATAATAAAACAAATCCAAAACCCAACGTCTGAGGACTATACTTGTATCTTTAACATTGAAATATTCATGCACTTGAAGATTTTAGGTCTTCTAGACATTTTCACAAACACTTGAAGATTTTAGGTCTTCTAGACATTTTCACAAACAGTTCGACAGCAAAAGTAATGCACTTCGATATCACAATCAACCAATGTAGTAGATTACAAAAACAAAGAGAGTGCATTGCTCACTGACCTTACATCCTCGAACGCCAGGGAAGTGATGGAGGAACTCGCAACCGCCATGTCGAAGCTCGCAAGATCTCTAGCAGGAGTTTTAGTGGAGAGTTTGGGGCACCGGAAGGAAGTATTTGAAGATATGTGCGATGAAAGCACATGCTTCCTCCGGTTGAATCACTACCCAATCTGTCCGTTTCCCGGCGAAGTTACCGGCCTGGTGCCCCACACCGATAGCGATTATCTGACCATACTTCATCAAGATTCCGGCGGCGGGCTTCAGCTGATGAAAGGATCGCAATGGGTGGCCGTGAAACCCATTCCGGAAGCCCTTGTCGTTAACATTGGAGATCTTCTACAGGTACAAACTGAAAGTCTTCCGAAGTATAATGGTTTGGTAGAGCAAGTAGAAAGTGGGTATAAAAATATAGTTTGAAATGCAGGCTTGGAGCAACGATACGTACAAGAGTGTGAAGCATAGGGTGGTGACTAACTCGGTGCGGGAGAGATATTCGACAGCATACTTCCTCTGCCCATCTAACCAGTCTGGAATCGGGAGTTGCCGGCAGCCTTCACCATACAGACACTTCACTTTTGGAGAGTACAGGAAGCAA

mRNA sequence

ATGTTTAAGGAAGATACCACAATGATGAACTCCAATCCACCACTCTTCCAACAATATGAGTTGCTTTTGCAATCCACAGCTCATTTACAGCCACTTCACAATGGCTATCAACCAACTAGCTCTGTCCTGGAGGAATGTCAGCTCCCTCTTGTCGATCTTAAAGGTTTGGAAAGTGGCAAAGAAAAAGAGAGGATGGCTTGCAGTAGAGAGATTTTTGAAGCATCTGCAGAATGGGGATTTTTCCAAGTGATTAACCATGGGATACGCTCAGAGCTGCTGAGCAGAATGAGCAAAGAACAAATGAAGTTGTTTGCGGTGCCCTTTGAGAAAAAATCTACAAGTGGGATTTTGGATAATTCTTACCGATGGGGAGCAGCCACTGCAACCCAACCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGACTGCTATGGCGACTTCATTCACTTAAGGGAAGTGATGGAGGAACTCGCAACCGCCATGTCGAAGCTCGCAAGATCTCTAGCAGGAGTTTTAGTGGAGAGTTTGGGGCACCGGAAGGAAGTATTTGAAGATATGTGCGATGAAAGCACATGCTTCCTCCGGTTGAATCACTACCCAATCTGTCCGTTTCCCGGCGAAGTTACCGGCCTGGTGCCCCACACCGATAGCGATTATCTGACCATACTTCATCAAGATTCCGGCGGCGGGCTTCAGCTGATGAAAGGATCGCAATGGGTGGCCGTGAAACCCATTCCGGAAGCCCTTGTCGTTAACATTGGAGATCTTCTACAGGCTTGGAGCAACGATACGTACAAGAGTGTGAAGCATAGGGTGGTGACTAACTCGGTGCGGGAGAGATATTCGACAGCATACTTCCTCTGCCCATCTAACCAGTCTGGAATCGGGAGTTGCCGGCAGCCTTCACCATACAGACACTTCACTTTTGGAGAGTACAGGAAGCAA

Coding sequence (CDS)

ATGTTTAAGGAAGATACCACAATGATGAACTCCAATCCACCACTCTTCCAACAATATGAGTTGCTTTTGCAATCCACAGCTCATTTACAGCCACTTCACAATGGCTATCAACCAACTAGCTCTGTCCTGGAGGAATGTCAGCTCCCTCTTGTCGATCTTAAAGGTTTGGAAAGTGGCAAAGAAAAAGAGAGGATGGCTTGCAGTAGAGAGATTTTTGAAGCATCTGCAGAATGGGGATTTTTCCAAGTGATTAACCATGGGATACGCTCAGAGCTGCTGAGCAGAATGAGCAAAGAACAAATGAAGTTGTTTGCGGTGCCCTTTGAGAAAAAATCTACAAGTGGGATTTTGGATAATTCTTACCGATGGGGAGCAGCCACTGCAACCCAACCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGACTGCTATGGCGACTTCATTCACTTAAGGGAAGTGATGGAGGAACTCGCAACCGCCATGTCGAAGCTCGCAAGATCTCTAGCAGGAGTTTTAGTGGAGAGTTTGGGGCACCGGAAGGAAGTATTTGAAGATATGTGCGATGAAAGCACATGCTTCCTCCGGTTGAATCACTACCCAATCTGTCCGTTTCCCGGCGAAGTTACCGGCCTGGTGCCCCACACCGATAGCGATTATCTGACCATACTTCATCAAGATTCCGGCGGCGGGCTTCAGCTGATGAAAGGATCGCAATGGGTGGCCGTGAAACCCATTCCGGAAGCCCTTGTCGTTAACATTGGAGATCTTCTACAGGCTTGGAGCAACGATACGTACAAGAGTGTGAAGCATAGGGTGGTGACTAACTCGGTGCGGGAGAGATATTCGACAGCATACTTCCTCTGCCCATCTAACCAGTCTGGAATCGGGAGTTGCCGGCAGCCTTCACCATACAGACACTTCACTTTTGGAGAGTACAGGAAGCAA
Homology
[Close]
BLAST of CmoCh04G000320 vs. Swiss-Prot
Match: G2OX8_ARATH (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 309.3 bits (791), Expect = 5.0e-83
Identity = 149/286 (52.10%), Postives = 196/286 (68.53%), Query Frame = 1 

Query: 40  SSVLEECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKE 99
           ++V+EE +LP++D+  L  G E+ER  C   I  AS EWGFFQVINHGI  ++L +M +E
Sbjct: 34  TAVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQE 93

Query: 100 QMKLFAVPFEKKSTSGILD-NSYRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGDFIH 159
           Q+++F  PF+KKS S      SYRWG  +AT   Q SWSEAFH+P+T +S+     DF  
Sbjct: 94  QIRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFTT 153

Query: 160 LREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVT 219
           L   ME+ A+    LA  LA VL E  G     F++ C  +TC+LR+N YP CP P EV 
Sbjct: 154 LSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVY 213

Query: 220 GLVPHTDSDYLTILHQDSGGGLQLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVK 279
           GL+PHTDSD+LTIL+QD  GGLQL+K ++W+AVKP P+AL++NIGDL QAWSN  YKSV+
Sbjct: 214 GLMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVE 273

Query: 280 HRVVTNSVRERYSTAYFLCPSNQSGIGSCRQPSPYRHFTFGEYRKQ 325
           HRV+TN   ER+STAYF+CPS  + I        YR+F+F E+R+Q
Sbjct: 274 HRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQ 316

[Close]
BLAST of CmoCh04G000320 vs. Swiss-Prot
Match: G2OX7_ARATH (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 2.8e-62
Identity = 129/319 (40.44%), Postives = 186/319 (58.31%), Query Frame = 1 

Query: 9   MNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMACS 68
           M S PP    +  +  S+       +    ++      +LP++DL  L SG+E +R  C 
Sbjct: 1   MASQPPFKTNFCSIFGSSFPNSTSESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCV 60

Query: 69  REIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILD---NSYRWGA 128
           +++  A+ EWGFFQ++NHGI  ++   M  E+ KLF  PF  K      D   NSYRWG 
Sbjct: 61  KQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQPFSVKVRERFSDLSKNSYRWGN 120

Query: 129 ATATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESL 188
            +AT P Q+S SEAFHI L++VS      D  +LR ++E     ++++A+ +  +L + +
Sbjct: 121 PSATSPAQYSVSEAFHIILSEVSRIS--DDRNNLRTIVETYVQEIARVAQMICEILGKQV 180

Query: 189 GHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLQLMKG 248
               E FE++ +    FLRLN Y    F  EV GLVPHTD+ +LTIL QD  GGL+L   
Sbjct: 181 NVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENN 240

Query: 249 SQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNQSGIG 308
            QW++VKP  EAL VNIGD+ QA SN  Y+SV+HRV++ +  ER S A+F+CP  ++ I 
Sbjct: 241 GQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPANIERMSIAFFVCPYLETEID 300

Query: 309 SCRQPSPYRHFTFGEYRKQ 325
               P  YR F+F EY++Q
Sbjct: 301 CFGYPKKYRRFSFREYKEQ 317

[Close]
BLAST of CmoCh04G000320 vs. Swiss-Prot
Match: GAOX3_ARATH (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana GN=GA20OX3 PE=2 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.7e-38
Identity = 108/322 (33.54%), Postives = 159/322 (49.38%), Query Frame = 1 

Query: 3   KEDTTMMNSNPPLFQQYELLLQSTAHL--QPLHNGYQPTSSVLEECQLPLVDLKGLESGK 62
           KED+++ ++        +LL Q + H+  Q +   ++  S+ ++  Q+PL+DL G  SG 
Sbjct: 19  KEDSSIFDA--------KLLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGD 78

Query: 63  EKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNS 122
                  +R + +A+ + GFF + NHG+   LLSR        F  P  +K  +      
Sbjct: 79  SCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACEKQKA-----Q 138

Query: 123 YRWGAATA-----------------TQPNQFSWSEAFHIPLTK--VSE--ADCYGDFIHL 182
            +WG ++                  T   +FS  E  H    K  VS+   D Y DF   
Sbjct: 139 RKWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIHSQTVKDFVSKKMGDGYEDF--- 198

Query: 183 REVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTG 242
            +V +E A AM+ L+  +  +L  SLG  +  F++  ++S    RLN+YP C  P    G
Sbjct: 199 GKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELALG 258

Query: 243 LVPHTDSDYLTILHQDSGGGLQLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKH 302
             PH D   LTILHQD  GGLQ+   ++W ++ P P A VVNIGD   A +N  YKS  H
Sbjct: 259 TGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYKSCLH 318

[Close]
BLAST of CmoCh04G000320 vs. Swiss-Prot
Match: GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 160.2 bits (404), Expect = 3.7e-38
Identity = 103/276 (37.32%), Postives = 142/276 (51.45%), Query Frame = 1 

Query: 37  QPTSSVLEECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRM 96
           +P+ +VLE   +PL+DL+ L S      +  SR I EA  + GFF V+NHGI  EL+S  
Sbjct: 51  KPSINVLE-LDVPLIDLQNLLSDPSST-LDASRLISEACKKHGFFLVVNHGISEELISDA 110

Query: 97  SKEQMKLFAVPFEKKSTSGILDNSYRWGAATATQPNQFS----WSEAFHIPLT-----KV 156
            +   + F +P  +K    +L  S       ++   +FS    W E              
Sbjct: 111 HEYTSRFFDMPLSEKQR--VLRKSGESVGYASSFTGRFSTKLPWKETLSFRFCDDMSRSK 170

Query: 157 SEADCYGD-----FIHLREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCF 216
           S  D + D     F    +V +E   AMS L+  +  +L  SLG +++ F +  +E+   
Sbjct: 171 SVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFREFFEENDSI 230

Query: 217 LRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLQLMKGSQWVAVKPIPEALVVNI 276
           +RLN+YP C  P    G  PH D   LTILHQD   GLQ+   +QW +++P P+A VVNI
Sbjct: 231 MRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNPKAFVVNI 290

Query: 277 GDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCP 299
           GD   A SND YKS  HR V NS  ER S A+FLCP
Sbjct: 291 GDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCP 322

[Close]
BLAST of CmoCh04G000320 vs. Swiss-Prot
Match: GAO1B_WHEAT (Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 6.4e-38
Identity = 104/300 (34.67%), Postives = 142/300 (47.33%), Query Frame = 1 

Query: 35  GYQPTSSVLEECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLS 94
           G  PT    EE  +PL+D+ G+ SG  +     +R + EA    GFFQV+NHGI +ELL+
Sbjct: 26  GESPTPDAAEELHVPLIDIGGMLSGDPRATAEVTRLVGEACERHGFFQVVNHGIDAELLA 85

Query: 95  RMSKEQMKLFAVPF-EKKSTSGILDNSYRWGAA-TATQPNQFSWSEAFHI------PLTK 154
              +     F +P  EK+        S  + ++ T    ++  W E          P   
Sbjct: 86  DAHRCVDAFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKETLSFRSCPSDPALV 145

Query: 155 VSE--ADCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLR 214
           V    A    D   L EV     + MS+L+  +  VL ESLG  +  +    + +   +R
Sbjct: 146 VDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAHYRRFFEGNDSIMR 205

Query: 215 LNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLQLMKGSQWVAVKPIPEALVVNIGD 274
           LN+YP C  P E  G  PH D   LTILHQD+ GGLQ+    +W +++P  +A VVNIGD
Sbjct: 206 LNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPRADAFVVNIGD 265

Query: 275 LLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNQSGIG------SCRQPSPYRHFTF 319
              A SN  YKS  HR V NS   R S A+FLCP     +           P  Y  FT+
Sbjct: 266 TFMALSNGRYKSCLHRAVVNSKVPRKSLAFFLCPEMDKVVAPPGTLVDAANPRAYPDFTW 325

[Close]
BLAST of CmoCh04G000320 vs. TrEMBL
Match: A0A0A0KIW0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G052540 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 2.5e-158
Identity = 269/325 (82.77%), Postives = 295/325 (90.77%), Query Frame = 1 

Query: 1   MFKEDTT-MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESG 60
           MFKE+TT MM+SNPPLF QYE LLQSTAHLQP +NG  PT+SV+EECQLPL+DLKGL+S 
Sbjct: 1   MFKENTTMMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSS 60

Query: 61  KEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDN 120
            EKER+AC REIFEAS EWGFFQVINHGI +ELL+RM+KEQ+KLF VPFEKK TSGILDN
Sbjct: 61  DEKERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDN 120

Query: 121 SYRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAG 180
           SYRWG  TAT PNQFSWSEAFHIPLTKVSEA CYGDFI+LREVMEE+A+AMSK+AR LAG
Sbjct: 121 SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAG 180

Query: 181 VLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGG 240
           VLVES+G RKE+ ED+CDESTCFLRLNHYPICPF GEV+GLVPHTDSD+LTILHQDSGGG
Sbjct: 181 VLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGG 240

Query: 241 LQLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPS 300
           LQ+MKGSQW+AVKP P+ALVVNIGDLLQAWSNDTYKS KHRVV N +RERYS AYFLCPS
Sbjct: 241 LQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHRVVANPMRERYSIAYFLCPS 300

Query: 301 NQSGIGSCRQPSPYRHFTFGEYRKQ 325
             S IGSCR+PSPYRHFTF EYRKQ
Sbjct: 301 YHSQIGSCREPSPYRHFTFAEYRKQ 325

[Close]
BLAST of CmoCh04G000320 vs. TrEMBL
Match: W9SYI1_9ROSA (Gibberellin 2-beta-dioxygenase 8 OS=Morus notabilis GN=L484_014953 PE=3 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 2.8e-117
Identity = 206/317 (64.98%), Postives = 246/317 (77.60%), Query Frame = 1 

Query: 8   MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMAC 67
           +++SNPPL   Y+ LL  ++ LQ             EECQLP++DL GL+S +  ER+ C
Sbjct: 2   IIDSNPPLLDHYKALLDHSSTLQTAECDGHFDGVAAEECQLPMIDLNGLKSDEALERVEC 61

Query: 68  SREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAAT 127
           ++ I +A++EWGFFQV+NHGI  EL+ +M KEQ KLF  PFE K+TS +L+NSYRWG  T
Sbjct: 62  AKAISKAASEWGFFQVVNHGISLELVKKMRKEQTKLFEAPFEDKATSRLLNNSYRWGTPT 121

Query: 128 ATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGH 187
           AT+P+QFSWSEAFHIPLTKVSE DCYGDFI LRE  EE A AMSKLARSLAGVL E+LGH
Sbjct: 122 ATRPSQFSWSEAFHIPLTKVSEEDCYGDFISLREPTEEFAAAMSKLARSLAGVLAENLGH 181

Query: 188 RKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLQLMKGSQ 247
           R++VFE++CDESTCFLRLN YP C    E+ GLVPHTDS +LTIL QD  GGLQLMK  +
Sbjct: 182 RRQVFEEICDESTCFLRLNRYPACAVSSEIFGLVPHTDSGFLTILRQDHVGGLQLMKDYK 241

Query: 248 WVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNQSGIGSC 307
           WV+VKP PEAL+VNIGDL QAWSND +KSV+HRV+TN + ERYS AYFLCPS  S IGSC
Sbjct: 242 WVSVKPNPEALIVNIGDLFQAWSNDVFKSVEHRVMTNKM-ERYSIAYFLCPSYDSLIGSC 301

Query: 308 RQPSPYRHFTFGEYRKQ 325
           R+PS YR FTF +YR Q
Sbjct: 302 REPSVYRKFTFRQYRSQ 317

[Close]
BLAST of CmoCh04G000320 vs. TrEMBL
Match: B9RY49_RICCO (Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_0814470 PE=3 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 8.2e-117
Identity = 210/317 (66.25%), Postives = 242/317 (76.34%), Query Frame = 1 

Query: 8   MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMAC 67
           M+ SNPPL   Y  L       +PL   + P S  +EECQLPLVDL  L+SG EKER AC
Sbjct: 1   MVESNPPLQLSYTALTHQIP--EPLER-HTPQSG-MEECQLPLVDLNCLKSGAEKERSAC 60

Query: 68  SREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAAT 127
           S  I  AS+EWGFFQV+NHGI  ELL  M +EQ+KLF  PF +K+T G+L+NSYRWG  T
Sbjct: 61  SSAISTASSEWGFFQVVNHGISPELLKNMRREQVKLFQTPFHRKATCGLLNNSYRWGTPT 120

Query: 128 ATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGH 187
           AT PNQFSWSEAFHIPLTK+SE  CYG+F  LREVM E A AMSKLA+ LAG+L E+LGH
Sbjct: 121 ATCPNQFSWSEAFHIPLTKISEQACYGEFTSLREVMMEFAAAMSKLAKLLAGILAENLGH 180

Query: 188 RKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLQLMKGSQ 247
            + VFE++C ES CFLRLN YP CP   E+ GLVPHTDSD+LTIL+QD  GGLQLMK S+
Sbjct: 181 PRAVFEEICHESNCFLRLNRYPSCPISPEMFGLVPHTDSDFLTILYQDEVGGLQLMKDSK 240

Query: 248 WVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNQSGIGSC 307
           WVAVKP PEAL+VNIGDL QAWSND YKSV+H+V+ N   ERYS AYFLCPS  S IGSC
Sbjct: 241 WVAVKPNPEALIVNIGDLFQAWSNDIYKSVEHKVMANGKMERYSVAYFLCPSYDSLIGSC 300

Query: 308 RQPSPYRHFTFGEYRKQ 325
           ++PS YR FTFGEYR+Q
Sbjct: 301 KEPSIYREFTFGEYREQ 313

[Close]
BLAST of CmoCh04G000320 vs. TrEMBL
Match: F6HG07_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g01650 PE=3 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.1e-116
Identity = 204/318 (64.15%), Postives = 244/318 (76.73%), Query Frame = 1 

Query: 7   TMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMA 66
           TM NSNPPL Q Y +L Q                + +EECQLPL+DL+GL S  E+ER+A
Sbjct: 23  TMTNSNPPLLQHYGVLCQHGETPAVQQRDCGNNGAAMEECQLPLIDLEGLWSESEEERLA 82

Query: 67  CSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAA 126
           C+  I  AS++WGFFQV+NHGIR ELLS M +EQ+KLF  PFE+K+   +LDNSYRWG  
Sbjct: 83  CASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWGTP 142

Query: 127 TATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESLG 186
           TAT P + SWSEAFHIPLTKVSE  CYG+F  LREVM+E A AMS LAR LAGVL ESLG
Sbjct: 143 TATCPKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAESLG 202

Query: 187 HRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLQLMKGS 246
           H+K VF+++CDE+TCFLRLN YP CP   EV GLVPHTDSD+LTIL+QD  GGLQLMK S
Sbjct: 203 HQKGVFDEICDENTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMKDS 262

Query: 247 QWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNQSGIGS 306
           +WVAVKP  + L+VNIGDL QAWSN+ YKSV+H+V+ N+++ERYS AYFLCPS  + IGS
Sbjct: 263 KWVAVKPNKDTLIVNIGDLFQAWSNNEYKSVEHQVMANAIKERYSIAYFLCPSYDAFIGS 322

Query: 307 CRQPSPYRHFTFGEYRKQ 325
           C +P+ YR FTFGEYR+Q
Sbjct: 323 CSEPTIYRKFTFGEYRQQ 340

[Close]
BLAST of CmoCh04G000320 vs. TrEMBL
Match: A5BF50_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_025459 PE=3 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 1.5e-115
Identity = 203/317 (64.04%), Postives = 242/317 (76.34%), Query Frame = 1 

Query: 8   MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMAC 67
           M NSNPPL Q Y +L Q                + +EECQLPL+DL+GL S  E+ER+AC
Sbjct: 1   MTNSNPPLLQHYGVLCQHGETPAVQQRDCGNNGAAMEECQLPLIDLEGLWSESEEERLAC 60

Query: 68  SREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAAT 127
           +  I  AS++WGFFQV+NHGIR ELLS M +EQ+KLF  PFE+K+   +LDNSYRWG  T
Sbjct: 61  ASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWGTPT 120

Query: 128 ATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGH 187
           AT+  + SWSEAFHIPLTKVSE  CYG+F  LREVM+E A AMS LAR LAGVL ESLGH
Sbjct: 121 ATRLKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAESLGH 180

Query: 188 RKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLQLMKGSQ 247
           +K VF+++CDESTCFLRLN YP CP   EV GLVPHTDSD+LTIL+QD  GGLQLMK S+
Sbjct: 181 QKGVFDEICDESTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMKDSK 240

Query: 248 WVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNQSGIGSC 307
           WVAVKP  + L+VNIGDL QAWSN+ YKSV+H+V+ N+++ERYS AYFLCPS  + IGSC
Sbjct: 241 WVAVKPNKDTLIVNIGDLFQAWSNNEYKSVEHQVMANAIKERYSIAYFLCPSYDAFIGSC 300

Query: 308 RQPSPYRHFTFGEYRKQ 325
            +P+ YR FTFGEYR Q
Sbjct: 301 SEPTIYRKFTFGEYRXQ 317

[Close]
BLAST of CmoCh04G000320 vs. TAIR10
Match: AT4G21200.1 (AT4G21200.1 gibberellin 2-oxidase 8)

HSP 1 Score: 309.3 bits (791), Expect = 2.8e-84
Identity = 149/286 (52.10%), Postives = 196/286 (68.53%), Query Frame = 1 

Query: 40  SSVLEECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKE 99
           ++V+EE +LP++D+  L  G E+ER  C   I  AS EWGFFQVINHGI  ++L +M +E
Sbjct: 34  TAVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQE 93

Query: 100 QMKLFAVPFEKKSTSGILD-NSYRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGDFIH 159
           Q+++F  PF+KKS S      SYRWG  +AT   Q SWSEAFH+P+T +S+     DF  
Sbjct: 94  QIRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFTT 153

Query: 160 LREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVT 219
           L   ME+ A+    LA  LA VL E  G     F++ C  +TC+LR+N YP CP P EV 
Sbjct: 154 LSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVY 213

Query: 220 GLVPHTDSDYLTILHQDSGGGLQLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVK 279
           GL+PHTDSD+LTIL+QD  GGLQL+K ++W+AVKP P+AL++NIGDL QAWSN  YKSV+
Sbjct: 214 GLMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVE 273

Query: 280 HRVVTNSVRERYSTAYFLCPSNQSGIGSCRQPSPYRHFTFGEYRKQ 325
           HRV+TN   ER+STAYF+CPS  + I        YR+F+F E+R+Q
Sbjct: 274 HRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQ 316

[Close]
BLAST of CmoCh04G000320 vs. TAIR10
Match: AT1G50960.1 (AT1G50960.1 gibberellin 2-oxidase 7)

HSP 1 Score: 240.4 bits (612), Expect = 1.6e-63
Identity = 129/319 (40.44%), Postives = 186/319 (58.31%), Query Frame = 1 

Query: 9   MNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMACS 68
           M S PP    +  +  S+       +    ++      +LP++DL  L SG+E +R  C 
Sbjct: 1   MASQPPFKTNFCSIFGSSFPNSTSESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCV 60

Query: 69  REIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILD---NSYRWGA 128
           +++  A+ EWGFFQ++NHGI  ++   M  E+ KLF  PF  K      D   NSYRWG 
Sbjct: 61  KQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQPFSVKVRERFSDLSKNSYRWGN 120

Query: 129 ATATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESL 188
            +AT P Q+S SEAFHI L++VS      D  +LR ++E     ++++A+ +  +L + +
Sbjct: 121 PSATSPAQYSVSEAFHIILSEVSRIS--DDRNNLRTIVETYVQEIARVAQMICEILGKQV 180

Query: 189 GHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLQLMKG 248
               E FE++ +    FLRLN Y    F  EV GLVPHTD+ +LTIL QD  GGL+L   
Sbjct: 181 NVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENN 240

Query: 249 SQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNQSGIG 308
            QW++VKP  EAL VNIGD+ QA SN  Y+SV+HRV++ +  ER S A+F+CP  ++ I 
Sbjct: 241 GQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPANIERMSIAFFVCPYLETEID 300

Query: 309 SCRQPSPYRHFTFGEYRKQ 325
               P  YR F+F EY++Q
Sbjct: 301 CFGYPKKYRRFSFREYKEQ 317

[Close]
BLAST of CmoCh04G000320 vs. TAIR10
Match: AT3G19000.1 (AT3G19000.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 171.8 bits (434), Expect = 7.0e-43
Identity = 103/299 (34.45%), Postives = 153/299 (51.17%), Query Frame = 1 

Query: 47  QLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAV 106
           ++P +DL  LE     ++ A ++EI EA   WGFFQVINHG+ S L  R+ K   + F +
Sbjct: 31  EIPTIDLSSLED-THHDKTAIAKEIAEACKRWGFFQVINHGLPSALRHRVEKTAAEFFNL 90

Query: 107 PFEKK--------STSGILDNSYRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGDFI- 166
             E+K        +  G  D  +        +   F   ++  +P +   E         
Sbjct: 91  TTEEKRKVKRDEVNPMGYHDEEHTKNVRDWKEIFDFFLQDSTIVPASPEPEDTELRKLTN 150

Query: 167 -------HLREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPI 226
                  H REV +E A  + KLA  L  ++  SLG   +      +E T FLR NHYP 
Sbjct: 151 QWPQNPSHFREVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNEQTSFLRFNHYPP 210

Query: 227 CPFPGEVTGLVPHTDSDYLTILHQDSGGGLQLMKGS--QWVAVKPIPEALVVNIGDLLQA 286
           CP P    G+  H D   LT+L QDS GGLQ+ + S  QW+ VKPI +AL++N+G+ +Q 
Sbjct: 211 CPNPELALGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIINMGNCIQV 270

Query: 287 WSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNQSGIG------SCRQPSPYRHFTFGEY 322
           W+ND Y S +HRVV N+ +ER+S  +F  PS+++ I       S   P  Y+ + +G++
Sbjct: 271 WTNDEYWSAEHRVVVNTSKERFSIPFFFFPSHEANIEPLEELISEENPPCYKKYNWGKF 328

[Close]
BLAST of CmoCh04G000320 vs. TAIR10
Match: AT5G07200.1 (AT5G07200.1 gibberellin 20-oxidase 3)

HSP 1 Score: 161.4 bits (407), Expect = 9.4e-40
Identity = 108/322 (33.54%), Postives = 159/322 (49.38%), Query Frame = 1 

Query: 3   KEDTTMMNSNPPLFQQYELLLQSTAHL--QPLHNGYQPTSSVLEECQLPLVDLKGLESGK 62
           KED+++ ++        +LL Q + H+  Q +   ++  S+ ++  Q+PL+DL G  SG 
Sbjct: 19  KEDSSIFDA--------KLLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGD 78

Query: 63  EKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNS 122
                  +R + +A+ + GFF + NHG+   LLSR        F  P  +K  +      
Sbjct: 79  SCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACEKQKA-----Q 138

Query: 123 YRWGAATA-----------------TQPNQFSWSEAFHIPLTK--VSE--ADCYGDFIHL 182
            +WG ++                  T   +FS  E  H    K  VS+   D Y DF   
Sbjct: 139 RKWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIHSQTVKDFVSKKMGDGYEDF--- 198

Query: 183 REVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTG 242
            +V +E A AM+ L+  +  +L  SLG  +  F++  ++S    RLN+YP C  P    G
Sbjct: 199 GKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELALG 258

Query: 243 LVPHTDSDYLTILHQDSGGGLQLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKH 302
             PH D   LTILHQD  GGLQ+   ++W ++ P P A VVNIGD   A +N  YKS  H
Sbjct: 259 TGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYKSCLH 318

[Close]
BLAST of CmoCh04G000320 vs. TAIR10
Match: AT4G25420.1 (AT4G25420.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 160.2 bits (404), Expect = 2.1e-39
Identity = 103/276 (37.32%), Postives = 142/276 (51.45%), Query Frame = 1 

Query: 37  QPTSSVLEECQLPLVDLKGLESGKEKERMACSREIFEASAEWGFFQVINHGIRSELLSRM 96
           +P+ +VLE   +PL+DL+ L S      +  SR I EA  + GFF V+NHGI  EL+S  
Sbjct: 51  KPSINVLE-LDVPLIDLQNLLSDPSST-LDASRLISEACKKHGFFLVVNHGISEELISDA 110

Query: 97  SKEQMKLFAVPFEKKSTSGILDNSYRWGAATATQPNQFS----WSEAFHIPLT-----KV 156
            +   + F +P  +K    +L  S       ++   +FS    W E              
Sbjct: 111 HEYTSRFFDMPLSEKQR--VLRKSGESVGYASSFTGRFSTKLPWKETLSFRFCDDMSRSK 170

Query: 157 SEADCYGD-----FIHLREVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCF 216
           S  D + D     F    +V +E   AMS L+  +  +L  SLG +++ F +  +E+   
Sbjct: 171 SVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFREFFEENDSI 230

Query: 217 LRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLQLMKGSQWVAVKPIPEALVVNI 276
           +RLN+YP C  P    G  PH D   LTILHQD   GLQ+   +QW +++P P+A VVNI
Sbjct: 231 MRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNPKAFVVNI 290

Query: 277 GDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCP 299
           GD   A SND YKS  HR V NS  ER S A+FLCP
Sbjct: 291 GDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCP 322

[Close]
BLAST of CmoCh04G000320 vs. NCBI nr
Match: gi|778698231|ref|XP_004142323.2| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus])

HSP 1 Score: 566.2 bits (1458), Expect = 3.6e-158
Identity = 269/325 (82.77%), Postives = 295/325 (90.77%), Query Frame = 1 

Query: 1   MFKEDTT-MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESG 60
           MFKE+TT MM+SNPPLF QYE LLQSTAHLQP +NG  PT+SV+EECQLPL+DLKGL+S 
Sbjct: 1   MFKENTTMMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSS 60

Query: 61  KEKERMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDN 120
            EKER+AC REIFEAS EWGFFQVINHGI +ELL+RM+KEQ+KLF VPFEKK TSGILDN
Sbjct: 61  DEKERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDN 120

Query: 121 SYRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAG 180
           SYRWG  TAT PNQFSWSEAFHIPLTKVSEA CYGDFI+LREVMEE+A+AMSK+AR LAG
Sbjct: 121 SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAG 180

Query: 181 VLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGG 240
           VLVES+G RKE+ ED+CDESTCFLRLNHYPICPF GEV+GLVPHTDSD+LTILHQDSGGG
Sbjct: 181 VLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGG 240

Query: 241 LQLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPS 300
           LQ+MKGSQW+AVKP P+ALVVNIGDLLQAWSNDTYKS KHRVV N +RERYS AYFLCPS
Sbjct: 241 LQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHRVVANPMRERYSIAYFLCPS 300

Query: 301 NQSGIGSCRQPSPYRHFTFGEYRKQ 325
             S IGSCR+PSPYRHFTF EYRKQ
Sbjct: 301 YHSQIGSCREPSPYRHFTFAEYRKQ 325

[Close]
BLAST of CmoCh04G000320 vs. NCBI nr
Match: gi|659109410|ref|XP_008454705.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X1 [Cucumis melo])

HSP 1 Score: 458.8 bits (1179), Expect = 8.1e-126
Identity = 217/262 (82.82%), Postives = 240/262 (91.60%), Query Frame = 1 

Query: 6   TTMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERM 65
           TTMM+SNPPLF QYE LLQSTAHLQP +NG  PT+SV+EECQLPL+DLKGL+S  EKER+
Sbjct: 48  TTMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSNEKERV 107

Query: 66  ACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGA 125
           AC REIFEAS EWGFFQVINHGI +ELL+RM+KEQ+KLF VPFEKK TSGILDNSYRWG 
Sbjct: 108 ACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKITSGILDNSYRWGT 167

Query: 126 ATATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESL 185
            TAT PNQ SWSEAFHIPLTKVSEA CYGDFI+LREVMEE+A+AMSK+AR LAGVLVES+
Sbjct: 168 PTATHPNQLSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLVESM 227

Query: 186 GHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLQLMKG 245
           G RKE+ ED+CDESTCFLRLNHYPICPF GEV+GLVPHTDSD+LTILHQDSGGGLQ+MKG
Sbjct: 228 GQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKG 287

Query: 246 SQWVAVKPIPEALVVNIGDLLQ 268
           SQW+AVKP P+ALVVNIGDLLQ
Sbjct: 288 SQWLAVKPNPQALVVNIGDLLQ 309

[Close]
BLAST of CmoCh04G000320 vs. NCBI nr
Match: gi|659109414|ref|XP_008454707.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X2 [Cucumis melo])

HSP 1 Score: 458.8 bits (1179), Expect = 8.1e-126
Identity = 217/262 (82.82%), Postives = 240/262 (91.60%), Query Frame = 1 

Query: 6   TTMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERM 65
           TTMM+SNPPLF QYE LLQSTAHLQP +NG  PT+SV+EECQLPL+DLKGL+S  EKER+
Sbjct: 7   TTMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSNEKERV 66

Query: 66  ACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGA 125
           AC REIFEAS EWGFFQVINHGI +ELL+RM+KEQ+KLF VPFEKK TSGILDNSYRWG 
Sbjct: 67  ACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKITSGILDNSYRWGT 126

Query: 126 ATATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESL 185
            TAT PNQ SWSEAFHIPLTKVSEA CYGDFI+LREVMEE+A+AMSK+AR LAGVLVES+
Sbjct: 127 PTATHPNQLSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLVESM 186

Query: 186 GHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLQLMKG 245
           G RKE+ ED+CDESTCFLRLNHYPICPF GEV+GLVPHTDSD+LTILHQDSGGGLQ+MKG
Sbjct: 187 GQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKG 246

Query: 246 SQWVAVKPIPEALVVNIGDLLQ 268
           SQW+AVKP P+ALVVNIGDLLQ
Sbjct: 247 SQWLAVKPNPQALVVNIGDLLQ 268

[Close]
BLAST of CmoCh04G000320 vs. NCBI nr
Match: gi|703159524|ref|XP_010112271.1| (Gibberellin 2-beta-dioxygenase 8 [Morus notabilis])

HSP 1 Score: 429.9 bits (1104), Expect = 4.0e-117
Identity = 206/317 (64.98%), Postives = 246/317 (77.60%), Query Frame = 1 

Query: 8   MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMAC 67
           +++SNPPL   Y+ LL  ++ LQ             EECQLP++DL GL+S +  ER+ C
Sbjct: 2   IIDSNPPLLDHYKALLDHSSTLQTAECDGHFDGVAAEECQLPMIDLNGLKSDEALERVEC 61

Query: 68  SREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAAT 127
           ++ I +A++EWGFFQV+NHGI  EL+ +M KEQ KLF  PFE K+TS +L+NSYRWG  T
Sbjct: 62  AKAISKAASEWGFFQVVNHGISLELVKKMRKEQTKLFEAPFEDKATSRLLNNSYRWGTPT 121

Query: 128 ATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGH 187
           AT+P+QFSWSEAFHIPLTKVSE DCYGDFI LRE  EE A AMSKLARSLAGVL E+LGH
Sbjct: 122 ATRPSQFSWSEAFHIPLTKVSEEDCYGDFISLREPTEEFAAAMSKLARSLAGVLAENLGH 181

Query: 188 RKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLQLMKGSQ 247
           R++VFE++CDESTCFLRLN YP C    E+ GLVPHTDS +LTIL QD  GGLQLMK  +
Sbjct: 182 RRQVFEEICDESTCFLRLNRYPACAVSSEIFGLVPHTDSGFLTILRQDHVGGLQLMKDYK 241

Query: 248 WVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNQSGIGSC 307
           WV+VKP PEAL+VNIGDL QAWSND +KSV+HRV+TN + ERYS AYFLCPS  S IGSC
Sbjct: 242 WVSVKPNPEALIVNIGDLFQAWSNDVFKSVEHRVMTNKM-ERYSIAYFLCPSYDSLIGSC 301

Query: 308 RQPSPYRHFTFGEYRKQ 325
           R+PS YR FTF +YR Q
Sbjct: 302 REPSVYRKFTFRQYRSQ 317

[Close]
BLAST of CmoCh04G000320 vs. NCBI nr
Match: gi|255555559|ref|XP_002518816.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8 [Ricinus communis])

HSP 1 Score: 428.3 bits (1100), Expect = 1.2e-116
Identity = 210/317 (66.25%), Postives = 242/317 (76.34%), Query Frame = 1 

Query: 8   MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMAC 67
           M+ SNPPL   Y  L       +PL   + P S  +EECQLPLVDL  L+SG EKER AC
Sbjct: 1   MVESNPPLQLSYTALTHQIP--EPLER-HTPQSG-MEECQLPLVDLNCLKSGAEKERSAC 60

Query: 68  SREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAAT 127
           S  I  AS+EWGFFQV+NHGI  ELL  M +EQ+KLF  PF +K+T G+L+NSYRWG  T
Sbjct: 61  SSAISTASSEWGFFQVVNHGISPELLKNMRREQVKLFQTPFHRKATCGLLNNSYRWGTPT 120

Query: 128 ATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGH 187
           AT PNQFSWSEAFHIPLTK+SE  CYG+F  LREVM E A AMSKLA+ LAG+L E+LGH
Sbjct: 121 ATCPNQFSWSEAFHIPLTKISEQACYGEFTSLREVMMEFAAAMSKLAKLLAGILAENLGH 180

Query: 188 RKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLQLMKGSQ 247
            + VFE++C ES CFLRLN YP CP   E+ GLVPHTDSD+LTIL+QD  GGLQLMK S+
Sbjct: 181 PRAVFEEICHESNCFLRLNRYPSCPISPEMFGLVPHTDSDFLTILYQDEVGGLQLMKDSK 240

Query: 248 WVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNQSGIGSC 307
           WVAVKP PEAL+VNIGDL QAWSND YKSV+H+V+ N   ERYS AYFLCPS  S IGSC
Sbjct: 241 WVAVKPNPEALIVNIGDLFQAWSNDIYKSVEHKVMANGKMERYSVAYFLCPSYDSLIGSC 300

Query: 308 RQPSPYRHFTFGEYRKQ 325
           ++PS YR FTFGEYR+Q
Sbjct: 301 KEPSIYREFTFGEYREQ 313

The following BLAST results are available for this feature:
BLAST of CmoCh04G000320 vs. Swiss-Prot
Match NameE-valueIdentityDescription
G2OX8_ARATH5.0e-8352.10Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2[more]
G2OX7_ARATH2.8e-6240.44Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1[more]
GAOX3_ARATH1.7e-3833.54Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana GN=GA20OX3 PE=2 SV=1[more]
GAOX1_ARATH3.7e-3837.32Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2[more]
GAO1B_WHEAT6.4e-3834.67Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1[more]
BLAST of CmoCh04G000320 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KIW0_CUCSA2.5e-15882.77Uncharacterized protein OS=Cucumis sativus GN=Csa_5G052540 PE=3 SV=1[more]
W9SYI1_9ROSA2.8e-11764.98Gibberellin 2-beta-dioxygenase 8 OS=Morus notabilis GN=L484_014953 PE=3 SV=1[more]
B9RY49_RICCO8.2e-11766.25Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_0814470 PE=3 SV=1[more]
F6HG07_VITVI1.1e-11664.15Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g01650 PE=3 SV=... [more]
A5BF50_VITVI1.5e-11564.04Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_025459 PE=3 SV=1[more]
BLAST of CmoCh04G000320 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G21200.12.8e-8452.10 gibberellin 2-oxidase 8[more]
AT1G50960.11.6e-6340.44 gibberellin 2-oxidase 7[more]
AT3G19000.17.0e-4334.45 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT5G07200.19.4e-4033.54 gibberellin 20-oxidase 3[more]
AT4G25420.12.1e-3937.32 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
BLAST of CmoCh04G000320 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778698231|ref|XP_004142323.2|3.6e-15882.77PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus][more]
gi|659109410|ref|XP_008454705.1|8.1e-12682.82PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X1 [Cucumis melo][more]
gi|659109414|ref|XP_008454707.1|8.1e-12682.82PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X2 [Cucumis melo][more]
gi|703159524|ref|XP_010112271.1|4.0e-11764.98Gibberellin 2-beta-dioxygenase 8 [Morus notabilis][more]
gi|255555559|ref|XP_002518816.1|1.2e-11666.25PREDICTED: gibberellin 2-beta-dioxygenase 8 [Ricinus communis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR026992DIOX_N
IPR027443IPNS-like
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0016491 oxidoreductase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G000320.1CmoCh04G000320.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 202..298
score: 5.1
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 197..298
score: 14
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 48..143
score: 1.0
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 37..323
score: 4.3
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 1..324
score: 6.1E
NoneNo IPR availablePANTHERPTHR10209:SF177SUBFAMILY NOT NAMEDcoord: 1..324
score: 6.1E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 33..323
score: 6.87

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmoCh04G000320CmaCh04G000330Cucurbita maxima (Rimu)cmacmoB728
CmoCh04G000320Cp4.1LG01g06230Cucurbita pepo (Zucchini)cmocpeB673
CmoCh04G000320MELO3C034260.2Melon (DHL92) v3.6.1cmomedB724
CmoCh04G000320Bhi07G000219Wax gourdcmowgoB0889
CmoCh04G000320Carg21988Silver-seed gourdcarcmoB0250
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CmoCh04G000320Cucurbita moschata (Rifu)cmocmoB464
CmoCh04G000320Cucurbita maxima (Rimu)cmacmoB731
CmoCh04G000320Wild cucumber (PI 183967)cmocpiB760
CmoCh04G000320Wild cucumber (PI 183967)cmocpiB744
CmoCh04G000320Cucumber (Chinese Long) v2cmocuB735
CmoCh04G000320Cucumber (Chinese Long) v2cmocuB756
CmoCh04G000320Watermelon (Charleston Gray)cmowcgB676
CmoCh04G000320Cucurbita pepo (Zucchini)cmocpeB674
CmoCh04G000320Bottle gourd (USVL1VR-Ls)cmolsiB673
CmoCh04G000320Cucumber (Gy14) v2cgybcmoB641
CmoCh04G000320Cucumber (Gy14) v2cgybcmoB647
CmoCh04G000320Silver-seed gourdcarcmoB0924
CmoCh04G000320Cucumber (Chinese Long) v3cmocucB0862
CmoCh04G000320Cucumber (Chinese Long) v3cmocucB0867
CmoCh04G000320Watermelon (97103) v2cmowmbB755