Cp4.1LG01g07810 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG01g07810
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Gibberellin 2-oxidase
Location	Cp4.1LG01 : 5278368 .. 5280625 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTAGCTCCTTTACAGTATCCTCTTCTATTGTCTTTGTTTGATTCCACTTGTGATCTTCCTCCTCCCCACAACTTAAGCCCAACTTCTCTTTTCCAAAGACAGGGAATATAAAGAGCACTTCTCGCTTTCATAACTTTTGTAGGAAACCCACAAGGAATATAGAGAACACTACCATGACGGACTCCAACCCACCACTCTTCCATCGATATGAGTTGCTTTTGCAATCCAAAGCTCAATTACAACTACTTAACAATGGCAATCAACAAATTAACTCTGTCATGGAGGAATGTCAGCTTCCTCTTATTGATCTTACAGGTTTGGAAAGTAGCAATGAAAAGGAGAGGAGGGCTTGCAGTAGAGAAATTTTTGGAGCGTCGGCAGAATGGGGATTCTTCCAAGTGATAAACCATGGGATACGCACAGAGCTGCTGAGCAGAATGAGCAAAGAACAAATGAAGTTATTTGGGGTACCATTTGAGAAAAAATCTACAAGTGGGATTTTGGATCATTCTTACAGATGGGGAACACCAACTGCAGCCCACCCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGCCTGCTATGGCGAGTTCATTAACTTAAGGTATCAACTTTACCACCCTTCATCCCAGCTACATACTTCTGTTACGTATTGTATTTTTTTCATTTAGTTCCAAGACTTGAAACGTTGTGTCGATATGATCTCTTAGACTTAAAAAGGTTCAATTATATTCTTAAGTTTTAGACTAATTTCAGATTACGTTTTTGTTTGTTAATATCACATTTACAGAAGGATGGTATGGCATGCTTGATAATTTATCCAACCCTATGCCATACCATGCCAACTCAGCCAAAAGTCATCTAATGATCATGTCATATTCATATTTTAGTTCAAATTTGAGGAACATAAATGAAAATTTCAAAAGTTCTGTTTGTAATCTAACCTGATATCTTTTATATTATAATTTTTAATGCACTTTTCACCAACATCCCGCAATCTTGAAGAGTTTAACTCGTCTAGACATTTTCACAAACAGCTAGACCGCAAAAAGTAATAGCACCTCGAAATCACAATCAACCGGCATTAAATGGCCACATTCTTCCACAATTGAACTAAATTACAAAAAAAAAAAAAAAAAAGGAGAGCCCACTACTCACTGACCTTATATGCTTGAACGCCAGGGAAGTGATGGAGGAACTGGCAACCGCCATGTCGAAGTTAGCAAGATCTTTAGCAGGAGTTTTAGTGGAGTGTTTGGGCCACCGAAAAGAAATATTTGACGATATCTGCGACGAAAGCACATGCTTCCTCCGATTGAATCATTACCCAATCTGTCCATTTCCCGGCGAAGTTTCCGGCCTGGTGCCCCACACGGACAGCGATTTTCTTACCATACTTCATCAAGATCGCAGCGGCGGGCTGCAGCTGATGAAAGGATCGCAATGGGTAGCTGTAAAACCCAATCCAGAAGCCCTTGTTGTCAACATTGGAGATCTTCTACAGGTACGCGCTGCAACTCTTTTACTTCGTTTTGATTATGACTCTAGGGTTAAATTTGTAAATTGGTCTAGGTGGTTTGACGAGGTTAGATTCAATTTAATCTATTTTTGTTTTATTTTCTATTTTCAACCCTTAAAACAAAATTTAAAACACCAAAGCATATATTCCTTTTTAATTTCAAAAGTTCAATTTTGTCCCTAATAAATTATTTAAATCATAAAATCAATTATTGTAACGTGACAATGTACCTAAAATTTTAAAAGTATTATAATAAAACCATACCAATTCGATTTAGTCAAGTTGGTTGGTCATCGGCTTCTGCAATTATATCATCCAAAATAAAAAATAGACGTTGGTTTAAAATATATTTATCGATCGACAAATAATTCAACCCTTTTCAATTTCGAACTATAAGTCTTCTTACTTTATGCAAAATATCATGAACTGATAAGCTAATCTCCAGGTGTGGAGCAATGATACTTACAAAAGTGTGAAGCACAGGGTCGTGACTAATTCAATGCGGGAAAGATATTCGATAGCGTACTTCCTCTGCCCTTCTTATCATTCTCCAATTGGGAGTTGCAGACAACCTTCACCGTATAGACACTTCACTTTCGCTGAGTACAGAAAGCAAGTTAGAGATGATGTGGAAAAAATTGGATACAAAGTAGGTCTTTCAAAATTTCGGCTCGTCAATATCAATACCAATTCAAACTCAAATAACTAG

mRNA sequence

ATGGTAGCTCCTTTACAGAAACCCACAAGGAATATAGAGAACACTACCATGACGGACTCCAACCCACCACTCTTCCATCGATATGAGTTGCTTTTGCAATCCAAAGCTCAATTACAACTACTTAACAATGGCAATCAACAAATTAACTCTGTCATGGAGGAATGTCAGCTTCCTCTTATTGATCTTACAGGTTTGGAAAGTAGCAATGAAAAGGAGAGGAGGGCTTGCAGTAGAGAAATTTTTGGAGCGTCGGCAGAATGGGGATTCTTCCAAGTGATAAACCATGGGATACGCACAGAGCTGCTGAGCAGAATGAGCAAAGAACAAATGAAGTTATTTGGGGTACCATTTGAGAAAAAATCTACAAGTGGGATTTTGGATCATTCTTACAGATGGGGAACACCAACTGCAGCCCACCCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGCCTGCTATGGCGAGTTCATTAACTTAAGGGAAGTGATGGAGGAACTGGCAACCGCCATGTCGAAGTTAGCAAGATCTTTAGCAGGAGTTTTAGTGGAGTGTTTGGGCCACCGAAAAGAAATATTTGACGATATCTGCGACGAAAGCACATGCTTCCTCCGATTGAATCATTACCCAATCTGTCCATTTCCCGGCGAAGTTTCCGGCCTGGTGCCCCACACGGACAGCGATTTTCTTACCATACTTCATCAAGATCGCAGCGGCGGGCTGCAGCTGATGAAAGGATCGCAATGGGTAGCTGTAAAACCCAATCCAGAAGCCCTTGTTGTCAACATTGGAGATCTTCTACAGGTGTGGAGCAATGATACTTACAAAAGTGTGAAGCACAGGGTCGTGACTAATTCAATGCGGGAAAGATATTCGATAGCGTACTTCCTCTGCCCTTCTTATCATTCTCCAATTGGGAGTTGCAGACAACCTTCACCGTATAGACACTTCACTTTCGCTGAGTACAGAAAGCAAGTTAGAGATGATGTGGAAAAAATTGGATACAAAGTAGGTCTTTCAAAATTTCGGCTCGTCAATATCAATACCAATTCAAACTCAAATAACTAG

Coding sequence (CDS)

ATGGTAGCTCCTTTACAGAAACCCACAAGGAATATAGAGAACACTACCATGACGGACTCCAACCCACCACTCTTCCATCGATATGAGTTGCTTTTGCAATCCAAAGCTCAATTACAACTACTTAACAATGGCAATCAACAAATTAACTCTGTCATGGAGGAATGTCAGCTTCCTCTTATTGATCTTACAGGTTTGGAAAGTAGCAATGAAAAGGAGAGGAGGGCTTGCAGTAGAGAAATTTTTGGAGCGTCGGCAGAATGGGGATTCTTCCAAGTGATAAACCATGGGATACGCACAGAGCTGCTGAGCAGAATGAGCAAAGAACAAATGAAGTTATTTGGGGTACCATTTGAGAAAAAATCTACAAGTGGGATTTTGGATCATTCTTACAGATGGGGAACACCAACTGCAGCCCACCCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGCCTGCTATGGCGAGTTCATTAACTTAAGGGAAGTGATGGAGGAACTGGCAACCGCCATGTCGAAGTTAGCAAGATCTTTAGCAGGAGTTTTAGTGGAGTGTTTGGGCCACCGAAAAGAAATATTTGACGATATCTGCGACGAAAGCACATGCTTCCTCCGATTGAATCATTACCCAATCTGTCCATTTCCCGGCGAAGTTTCCGGCCTGGTGCCCCACACGGACAGCGATTTTCTTACCATACTTCATCAAGATCGCAGCGGCGGGCTGCAGCTGATGAAAGGATCGCAATGGGTAGCTGTAAAACCCAATCCAGAAGCCCTTGTTGTCAACATTGGAGATCTTCTACAGGTGTGGAGCAATGATACTTACAAAAGTGTGAAGCACAGGGTCGTGACTAATTCAATGCGGGAAAGATATTCGATAGCGTACTTCCTCTGCCCTTCTTATCATTCTCCAATTGGGAGTTGCAGACAACCTTCACCGTATAGACACTTCACTTTCGCTGAGTACAGAAAGCAAGTTAGAGATGATGTGGAAAAAATTGGATACAAAGTAGGTCTTTCAAAATTTCGGCTCGTCAATATCAATACCAATTCAAACTCAAATAACTAG

Protein sequence

MVAPLQKPTRNIENTTMTDSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGTPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRLVNINTNSNSNN

Homology

[Close]

BLAST of Cp4.1LG01g07810 vs. Swiss-Prot
Match: G2OX8_ARATH (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 336.3 bits (861), Expect = 4.3e-91
Identity = 160/304 (52.63%), Postives = 212/304 (69.74%), Query Frame = 1


Query: 48  INSVMEECQLPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSK 107
           + +V+EE +LP+ID++ L    E+ER  C   I  AS EWGFFQVINHGI  ++L +M +
Sbjct: 33  VTAVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQ 92

Query: 108 EQMKLFGVPFEKKSTSGILDH-SYRWGTPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFI 167
           EQ+++F  PF+KKS S      SYRWGTP+A    Q SWSEAFH+P+T +S+     +F 
Sbjct: 93  EQIRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFT 152

Query: 168 NLREVMEELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGEV 227
            L   ME+ A+    LA  LA VL E  G     F + C  +TC+LR+N YP CP P EV
Sbjct: 153 TLSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEV 212

Query: 228 SGLVPHTDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSV 287
            GL+PHTDSDFLTIL+QD+ GGLQL+K ++W+AVKPNP+AL++NIGDL Q WSN  YKSV
Sbjct: 213 YGLMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSV 272

Query: 288 KHRVVTNSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVG 347
           +HRV+TN   ER+S AYF+CPSY + I        YR+F+F E+R+QV++DV+K G+KVG
Sbjct: 273 EHRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVG 332

Query: 348 LSKF 351
           L +F
Sbjct: 333 LPRF 333

[Close]

BLAST of Cp4.1LG01g07810 vs. Swiss-Prot
Match: G2OX7_ARATH (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 3.0e-68
Identity = 133/298 (44.63%), Postives = 188/298 (63.09%), Query Frame = 1


Query: 56  QLPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGV 115
           +LP+IDL+ L S  E +R+ C +++  A+ EWGFFQ++NHGI  ++   M  E+ KLF  
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQ 98

Query: 116 PFEKKSTSGILD---HSYRWGTPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVM 175
           PF  K      D   +SYRWG P+A  P Q+S SEAFHI L++VS  +   +  NLR ++
Sbjct: 99  PFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRIS--DDRNNLRTIV 158

Query: 176 EELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPH 235
           E     ++++A+ +  +L + +    E F++I +    FLRLN Y    F  EV GLVPH
Sbjct: 159 ETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPH 218

Query: 236 TDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVT 295
           TD+ FLTIL QD+ GGL+L    QW++VKP  EAL VNIGD+ Q  SN  Y+SV+HRV++
Sbjct: 219 TDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVIS 278

Query: 296 NSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKF 351
            +  ER SIA+F+CP   + I     P  YR F+F EY++Q   DV++ G KVGLS+F
Sbjct: 279 PANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRF 334

[Close]

BLAST of Cp4.1LG01g07810 vs. Swiss-Prot
Match: GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 156.8 bits (395), Expect = 4.6e-37
Identity = 105/300 (35.00%), Postives = 148/300 (49.33%), Query Frame = 1


Query: 22  PPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRACSREIF 81
           P +F+   L LQ+    Q +   +++ +  + E  +PLIDL  L S       A SR I 
Sbjct: 26  PLIFNPSMLNLQANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLSDPSSTLDA-SRLIS 85

Query: 82  GASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGTPTAAHPN 141
            A  + GFF V+NHGI  EL+S   +   + F +P  +K    +L  S       ++   
Sbjct: 86  EACKKHGFFLVVNHGISEELISDAHEYTSRFFDMPLSEKQR--VLRKSGESVGYASSFTG 145

Query: 142 QFS----WSEAFHIPLT-------KVSEAACYG---EFINLREVMEELATAMSKLARSLA 201
           +FS    W E               V +  C      F    +V +E   AMS L+  + 
Sbjct: 146 RFSTKLPWKETLSFRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIM 205

Query: 202 GVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSG 261
            +L   LG +++ F +  +E+   +RLN+YP C  P    G  PH D   LTILHQD   
Sbjct: 206 ELLGLSLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVN 265

Query: 262 GLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCP 308
           GLQ+   +QW +++PNP+A VVNIGD     SND YKS  HR V NS  ER S+A+FLCP
Sbjct: 266 GLQVFVENQWRSIRPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCP 322

[Close]

BLAST of Cp4.1LG01g07810 vs. Swiss-Prot
Match: DLO2_ARATH (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana GN=DLO2 PE=2 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.8e-36
Identity = 103/304 (33.88%), Postives = 148/304 (48.68%), Query Frame = 1


Query: 57  LPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVP 116
           +PLIDL  L   N   R     +   A +  GFFQ+ NHG+  E + +M     + F   
Sbjct: 42  IPLIDLHDLHGPN---RADIINQFAHACSSCGFFQIKNHGVPEETIKKMMNAAREFF--- 101

Query: 117 FEKKSTSGILDHSYRWGTPTAAHPNQFSWSEAFHIPLTKVSE------AACYG--EFIN- 176
             ++S S  + H       +A        S +F++   KVS         CY   +FIN 
Sbjct: 102 --RQSESERVKHY------SADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCYPIEDFINE 161

Query: 177 -------LREVMEELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPIC 236
                   REV  E AT++  L  +L   + E LG  K+   +   +    + +N+YP C
Sbjct: 162 WPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRC 221

Query: 237 PFPGEVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSN 296
           P P    GL  H D++ +T+L QD   GLQ+ K  +W+AV P P   +VN+GD +QV SN
Sbjct: 222 PQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISN 281

Query: 297 DTYKSVKHRVVTNSMRERYSIAYFLCPSYHSPIGSCRQ--------PSPYRHFTFAEYRK 337
           + YKSV HR V NS  ER SI  F CPS  + I   ++        P+ YR+FT+AEY +
Sbjct: 282 EKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEYFE 331

[Close]

BLAST of Cp4.1LG01g07810 vs. Swiss-Prot
Match: GAOX3_ARATH (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana GN=GA20OX3 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.5e-35
Identity = 102/315 (32.38%), Postives = 152/315 (48.25%), Query Frame = 1


Query: 13  ENTTMTDSNPPLFHRYELLLQSKAQL--QLLNNGNQQINSVMEECQLPLIDLTGLESSNE 72
           EN T  DS+  +F   +LL Q    +  Q +   +++ ++ ++  Q+PLIDL G  S + 
Sbjct: 15  ENKTKEDSS--IFDA-KLLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDS 74

Query: 73  KERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSY 132
                 +R +  A+ + GFF + NHG+   LLSR        F  P  +K  +       
Sbjct: 75  CLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACEKQKA-----QR 134

Query: 133 RWGTPTAAHPN-----------------QFSWSEAFHIPLTKVSEAACYGE-FINLREVM 192
           +WG  +    +                 +FS  E  H    K   +   G+ + +  +V 
Sbjct: 135 KWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIHSQTVKDFVSKKMGDGYEDFGKVY 194

Query: 193 EELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPH 252
           +E A AM+ L+  +  +L   LG  +  F +  ++S    RLN+YP C  P    G  PH
Sbjct: 195 QEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELALGTGPH 254

Query: 253 TDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVT 308
            D   LTILHQD+ GGLQ+   ++W ++ PNP A VVNIGD     +N  YKS  HR V 
Sbjct: 255 CDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYKSCLHRAVV 314

[Close]

BLAST of Cp4.1LG01g07810 vs. TrEMBL
Match: A0A0A0KIW0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G052540 PE=3 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 1.3e-168
Identity = 288/341 (84.46%), Postives = 307/341 (90.03%), Query Frame = 1


Query: 13  ENTTMT-DSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEK 72
           ENTTM  DSNPPLF +YE LLQS A LQ  NNGN   NSVMEECQLPLIDL GL+SS+EK
Sbjct: 4   ENTTMMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSDEK 63

Query: 73  ERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYR 132
           ER AC REIF AS EWGFFQVINHGI TELL+RM+KEQ+KLFGVPFEKK TSGILD+SYR
Sbjct: 64  ERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSYR 123

Query: 133 WGTPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLV 192
           WGTPTA HPNQFSWSEAFHIPLTKVSEAACYG+FI LREVMEE+A+AMSK+AR LAGVLV
Sbjct: 124 WGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLV 183

Query: 193 ECLGHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQL 252
           E +G RKE+ +DICDESTCFLRLNHYPICPF GEVSGLVPHTDSDFLTILHQD  GGLQ+
Sbjct: 184 ESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQV 243

Query: 253 MKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHS 312
           MKGSQW+AVKPNP+ALVVNIGDLLQ WSNDTYKS KHRVV N MRERYSIAYFLCPSYHS
Sbjct: 244 MKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHRVVANPMRERYSIAYFLCPSYHS 303

Query: 313 PIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRL 353
            IGSCR+PSPYRHFTFAEYRKQVRDDVEK GYK+GLS FRL
Sbjct: 304 QIGSCREPSPYRHFTFAEYRKQVRDDVEKTGYKIGLSNFRL 344

[Close]

BLAST of Cp4.1LG01g07810 vs. TrEMBL
Match: W9SYI1_9ROSA (Gibberellin 2-beta-dioxygenase 8 OS=Morus notabilis GN=L484_014953 PE=3 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 2.6e-127
Identity = 223/341 (65.40%), Postives = 264/341 (77.42%), Query Frame = 1


Query: 19  DSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRACSR 78
           DSNPPL   Y+ LL   + LQ             EECQLP+IDL GL+S    ER  C++
Sbjct: 4   DSNPPLLDHYKALLDHSSTLQTAECDGHFDGVAAEECQLPMIDLNGLKSDEALERVECAK 63

Query: 79  EIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGTPTAA 138
            I  A++EWGFFQV+NHGI  EL+ +M KEQ KLF  PFE K+TS +L++SYRWGTPTA 
Sbjct: 64  AISKAASEWGFFQVVNHGISLELVKKMRKEQTKLFEAPFEDKATSRLLNNSYRWGTPTAT 123

Query: 139 HPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGHRK 198
            P+QFSWSEAFHIPLTKVSE  CYG+FI+LRE  EE A AMSKLARSLAGVL E LGHR+
Sbjct: 124 RPSQFSWSEAFHIPLTKVSEEDCYGDFISLREPTEEFAAAMSKLARSLAGVLAENLGHRR 183

Query: 199 EIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQWV 258
           ++F++ICDESTCFLRLN YP C    E+ GLVPHTDS FLTIL QD  GGLQLMK  +WV
Sbjct: 184 QVFEEICDESTCFLRLNRYPACAVSSEIFGLVPHTDSGFLTILRQDHVGGLQLMKDYKWV 243

Query: 259 AVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSPIGSCRQ 318
           +VKPNPEAL+VNIGDL Q WSND +KSV+HRV+TN M ERYSIAYFLCPSY S IGSCR+
Sbjct: 244 SVKPNPEALIVNIGDLFQAWSNDVFKSVEHRVMTNKM-ERYSIAYFLCPSYDSLIGSCRE 303

Query: 319 PSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRLVNINTNS 360
           PS YR FTF +YR QV++DV+K+GYKVGLS+F L + +T++
Sbjct: 304 PSVYRKFTFRQYRSQVQEDVKKMGYKVGLSRFLLQDESTHT 343

[Close]

BLAST of Cp4.1LG01g07810 vs. TrEMBL
Match: F6HG07_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g01650 PE=3 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 1.3e-126
Identity = 223/339 (65.78%), Postives = 267/339 (78.76%), Query Frame = 1


Query: 16  TMTDSNPPLFHRYELLLQ--SKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKER 75
           TMT+SNPPL   Y +L Q      +Q  + GN    + MEECQLPLIDL GL S +E+ER
Sbjct: 23  TMTNSNPPLLQHYGVLCQHGETPAVQQRDCGNN--GAAMEECQLPLIDLEGLWSESEEER 82

Query: 76  RACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWG 135
            AC+  I  AS++WGFFQV+NHGIR ELLS M +EQ+KLF  PFE+K+   +LD+SYRWG
Sbjct: 83  LACASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWG 142

Query: 136 TPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVEC 195
           TPTA  P + SWSEAFHIPLTKVSE ACYGEF +LREVM+E A AMS LAR LAGVL E 
Sbjct: 143 TPTATCPKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAES 202

Query: 196 LGHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLMK 255
           LGH+K +FD+ICDE+TCFLRLN YP CP   EV GLVPHTDSDFLTIL+QD  GGLQLMK
Sbjct: 203 LGHQKGVFDEICDENTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMK 262

Query: 256 GSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSPI 315
            S+WVAVKPN + L+VNIGDL Q WSN+ YKSV+H+V+ N+++ERYSIAYFLCPSY + I
Sbjct: 263 DSKWVAVKPNKDTLIVNIGDLFQAWSNNEYKSVEHQVMANAIKERYSIAYFLCPSYDAFI 322

Query: 316 GSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRL 353
           GSC +P+ YR FTF EYR+QV++DV+K G+KVGL +F L
Sbjct: 323 GSCSEPTIYRKFTFGEYRQQVQEDVKKTGHKVGLPRFLL 359

[Close]

BLAST of Cp4.1LG01g07810 vs. TrEMBL
Match: A5BF50_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_025459 PE=3 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 1.8e-125
Identity = 222/338 (65.68%), Postives = 264/338 (78.11%), Query Frame = 1


Query: 17  MTDSNPPLFHRYELLLQ--SKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERR 76
           MT+SNPPL   Y +L Q      +Q  + GN    + MEECQLPLIDL GL S +E+ER 
Sbjct: 1   MTNSNPPLLQHYGVLCQHGETPAVQQRDCGNN--GAAMEECQLPLIDLEGLWSESEEERL 60

Query: 77  ACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGT 136
           AC+  I  AS++WGFFQV+NHGIR ELLS M +EQ+KLF  PFE+K+   +LD+SYRWGT
Sbjct: 61  ACASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWGT 120

Query: 137 PTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECL 196
           PTA    + SWSEAFHIPLTKVSE ACYGEF +LREVM+E A AMS LAR LAGVL E L
Sbjct: 121 PTATRLKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAESL 180

Query: 197 GHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLMKG 256
           GH+K +FD+ICDESTCFLRLN YP CP   EV GLVPHTDSDFLTIL+QD  GGLQLMK 
Sbjct: 181 GHQKGVFDEICDESTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMKD 240

Query: 257 SQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSPIG 316
           S+WVAVKPN + L+VNIGDL Q WSN+ YKSV+H+V+ N+++ERYSIAYFLCPSY + IG
Sbjct: 241 SKWVAVKPNKDTLIVNIGDLFQAWSNNEYKSVEHQVMANAIKERYSIAYFLCPSYDAFIG 300

Query: 317 SCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRL 353
           SC +P+ YR FTF EYR QV++DV+K G+KVGL +F L
Sbjct: 301 SCSEPTIYRKFTFGEYRXQVQEDVKKTGHKVGLPRFLL 336

[Close]

BLAST of Cp4.1LG01g07810 vs. TrEMBL
Match: A0A061E9S8_THECC (Gibberellin 2-oxidase 8 OS=Theobroma cacao GN=TCM_011301 PE=3 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 3.1e-125
Identity = 223/346 (64.45%), Postives = 259/346 (74.86%), Query Frame = 1


Query: 17  MTDSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 76
           M  S+PPL + Y  LL    Q         Q   +MEECQLPLIDL GL S +E  R+AC
Sbjct: 1   MIHSDPPLLNHYGALLCRSTQCD-----EPQSRLIMEECQLPLIDLRGLSSRDEMVRKAC 60

Query: 77  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGTPT 136
           +  I+ AS+EWGFFQV+NHGI   LL +M  EQ+KLF  PFE+K++  +L++SY WGTPT
Sbjct: 61  AAAIYRASSEWGFFQVVNHGISPRLLEKMRSEQVKLFQTPFERKASCRLLNNSYTWGTPT 120

Query: 137 AAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 196
           A  P QFSWSEAFH+PLTKVS+ ACYGEF +LREVM E A AMSKLAR LAGVL E LGH
Sbjct: 121 ATCPKQFSWSEAFHVPLTKVSDEACYGEFTSLREVMTEFAAAMSKLARLLAGVLTENLGH 180

Query: 197 RKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 256
            KE+ D+ICDESTCFLRLNHYP CP   E+SGLVPHTDSDFLTIL QD+ GGLQL K S+
Sbjct: 181 GKEVIDNICDESTCFLRLNHYPACPISPEISGLVPHTDSDFLTILCQDQVGGLQLTKDSK 240

Query: 257 WVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSPIGSC 316
           WV VKPN +AL+VNIGDL Q WSND YKSV+H+VVTN+  ERYSIAYFLCPS+ S IGS 
Sbjct: 241 WVTVKPNQDALIVNIGDLFQAWSNDVYKSVEHKVVTNAKMERYSIAYFLCPSFDSSIGSF 300

Query: 317 RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRLVNINTNSNSN 363
           R+PS YR FTF EYR QV++DV K GYKVGL +F L      + SN
Sbjct: 301 REPSVYRKFTFREYRDQVQEDVRKTGYKVGLPRFLLKGTEDFTRSN 341

[Close]

BLAST of Cp4.1LG01g07810 vs. TAIR10
Match: AT4G21200.1 (AT4G21200.1 gibberellin 2-oxidase 8)

HSP 1 Score: 336.3 bits (861), Expect = 2.4e-92
Identity = 160/304 (52.63%), Postives = 212/304 (69.74%), Query Frame = 1


Query: 48  INSVMEECQLPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSK 107
           + +V+EE +LP+ID++ L    E+ER  C   I  AS EWGFFQVINHGI  ++L +M +
Sbjct: 33  VTAVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQ 92

Query: 108 EQMKLFGVPFEKKSTSGILDH-SYRWGTPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFI 167
           EQ+++F  PF+KKS S      SYRWGTP+A    Q SWSEAFH+P+T +S+     +F 
Sbjct: 93  EQIRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFT 152

Query: 168 NLREVMEELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGEV 227
            L   ME+ A+    LA  LA VL E  G     F + C  +TC+LR+N YP CP P EV
Sbjct: 153 TLSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEV 212

Query: 228 SGLVPHTDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSV 287
            GL+PHTDSDFLTIL+QD+ GGLQL+K ++W+AVKPNP+AL++NIGDL Q WSN  YKSV
Sbjct: 213 YGLMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSV 272

Query: 288 KHRVVTNSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVG 347
           +HRV+TN   ER+S AYF+CPSY + I        YR+F+F E+R+QV++DV+K G+KVG
Sbjct: 273 EHRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVG 332

Query: 348 LSKF 351
           L +F
Sbjct: 333 LPRF 333

[Close]

BLAST of Cp4.1LG01g07810 vs. TAIR10
Match: AT1G50960.1 (AT1G50960.1 gibberellin 2-oxidase 7)

HSP 1 Score: 260.4 bits (664), Expect = 1.7e-69
Identity = 133/298 (44.63%), Postives = 188/298 (63.09%), Query Frame = 1


Query: 56  QLPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGV 115
           +LP+IDL+ L S  E +R+ C +++  A+ EWGFFQ++NHGI  ++   M  E+ KLF  
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQ 98

Query: 116 PFEKKSTSGILD---HSYRWGTPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVM 175
           PF  K      D   +SYRWG P+A  P Q+S SEAFHI L++VS  +   +  NLR ++
Sbjct: 99  PFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRIS--DDRNNLRTIV 158

Query: 176 EELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPH 235
           E     ++++A+ +  +L + +    E F++I +    FLRLN Y    F  EV GLVPH
Sbjct: 159 ETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPH 218

Query: 236 TDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVT 295
           TD+ FLTIL QD+ GGL+L    QW++VKP  EAL VNIGD+ Q  SN  Y+SV+HRV++
Sbjct: 219 TDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVIS 278

Query: 296 NSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKF 351
            +  ER SIA+F+CP   + I     P  YR F+F EY++Q   DV++ G KVGLS+F
Sbjct: 279 PANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRF 334

[Close]

BLAST of Cp4.1LG01g07810 vs. TAIR10
Match: AT5G58660.1 (AT5G58660.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 170.6 bits (431), Expect = 1.7e-42
Identity = 119/372 (31.99%), Postives = 184/372 (49.46%), Query Frame = 1


Query: 16  TMTDSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVM---EECQLPLIDLTGLESSNEKE 75
           ++++S PP F R            + N+ N     V+   ++  +P+IDL  L+   E  
Sbjct: 2   SISESYPPAFRR------------VTNDENPPATPVIVQGKDINIPVIDLERLDK--EIL 61

Query: 76  RRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKS---TSGILDHS 135
           R AC         EWG F++ NHG+   L SR+ +    L  +PFEKK     +     S
Sbjct: 62  REACK--------EWGIFRLENHGVPLALTSRLQEISESLLSLPFEKKRELFAAVKSPLS 121

Query: 136 YRWGTPT----------AAHPNQFSWSEAFHIPLTKVSE------AACYGE-------FI 195
           Y WGTP            A  +  +  E F++PL+ +S       + C  +         
Sbjct: 122 YFWGTPALNRSGDALKRGAQASNLTMLEGFNVPLSSLSSLSKLPTSTCCDDDAQEEPKLE 181

Query: 196 NLREVMEELATAMSKLARSLAGVLVECL-----GHRKEIFDDICDESTCFLRLNHYPICP 255
           + R +MEE    ++++A SL   + + L     G+R+    +   EST  +R+  YP   
Sbjct: 182 SFRVLMEEYGKHITRIAVSLFEAIAQTLNLELSGNRRS---EYLSESTGLIRVYRYPQSS 241

Query: 256 FPG--EVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWS 315
                E  G+  HTDS  ++IL +D SGGL++MKG +W  VKP    L+VN+GD++Q  S
Sbjct: 242 EEAAREALGMEVHTDSSVISILREDESGGLEIMKGEEWFCVKPVANTLIVNLGDMMQAIS 301

Query: 316 NDTYKSVKHRV-VTNSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDV 351
           +D YKSV HRV   N   ER+S+ YF+ P     I S    S Y+ FT++++  QV+ DV
Sbjct: 302 DDEYKSVTHRVKKRNRKTERHSVCYFVFPKRDCVIKS----SNYKLFTYSDFEAQVQADV 344

[Close]

BLAST of Cp4.1LG01g07810 vs. TAIR10
Match: AT5G05600.1 (AT5G05600.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 165.2 bits (417), Expect = 7.3e-41
Identity = 102/293 (34.81%), Postives = 149/293 (50.85%), Query Frame = 1


Query: 57  LPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVP 116
           +P+IDL GL S            I  A   WGFFQV+NHG++ EL+    +   + F +P
Sbjct: 62  IPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMP 121

Query: 117 FEKKSTSGILDHSYR-WGTPTAAHPN-QFSWSEAFHIPLTK--VSEAACYGEFI-NLREV 176
              K T      +Y  +G+           WS+ + + L    + +   +  F   +REV
Sbjct: 122 VNAKETYSNSPRTYEGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREV 181

Query: 177 MEELATAMSKLARSLAGVLVECLGHRKEIFDDIC--DESTCFLRLNHYPICPFPGEVSGL 236
           ++E    + KL+  +  VL   LG +++ F +    +     LR+N+YP CP P    GL
Sbjct: 182 IDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELALGL 241

Query: 237 VPHTDSDFLTIL-HQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKH 296
            PH+D   +TIL   D+  GLQ+ K   W+ VKP+P A +VNIGD +Q+ SN TYKSV+H
Sbjct: 242 SPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEH 301

Query: 297 RVVTNSMRERYSIAYFLCPSYHSPIG------SCRQPSPYRHFTFAEYRKQVR 336
           RV+ NS +ER S+A+F  P    PI       S   P  Y   TF +YR  +R
Sbjct: 302 RVIVNSDKERVSLAFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYRLFIR 354

[Close]

BLAST of Cp4.1LG01g07810 vs. TAIR10
Match: AT3G19010.1 (AT3G19010.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 164.5 bits (415), Expect = 1.2e-40
Identity = 106/314 (33.76%), Postives = 153/314 (48.73%), Query Frame = 1


Query: 56  QLPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGV 115
           ++P+IDL+ L+   + +      EI  A  +WGFFQV+NHG+ ++   R+ K     F +
Sbjct: 27  EIPVIDLSRLDDPEDVQN--VISEIGDACEKWGFFQVLNHGVPSDARQRVEKTVKMFFDL 86

Query: 116 PFEKK---STSGILDHSYRWGTPTAAHPNQFSWSEAFHI----PLTKVSEAACYGEFINL 175
           P E+K       +    Y  G  T    N   W E F I    P+   S      E + L
Sbjct: 87  PMEEKIKVKRDDVNPVGYHDGEHTK---NVKDWKEVFDIYFKDPMVIPSTTDPEDEGLRL 146

Query: 176 ------------REVMEELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNH 235
                       RE  E  A    KLA  L  ++   LG  KE F D   E   F R+N 
Sbjct: 147 VYNKWPQSPSDFREACEVYARHAEKLAFKLLELISLSLGLPKERFHDYFKEQMSFFRINR 206

Query: 236 YPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ--WVAVKPNPEALVVNIGDL 295
           YP CP P    G+  H D+D +++L QD  GGLQ+ + S   W  ++P P ALV+NIG+ 
Sbjct: 207 YPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRRSDGVWFPIRPVPNALVINIGNC 266

Query: 296 LQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYH------SPIGSCRQPSPYRHFTFA 342
           +++W+ND Y S +HRVV N+ RERYSI +FL PS+         + S   P  Y+ + + 
Sbjct: 267 MEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHDVEVKPLEELVSPENPPKYKGYKWG 326

[Close]

BLAST of Cp4.1LG01g07810 vs. NCBI nr
Match: gi|778698231|ref|XP_004142323.2| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus])

HSP 1 Score: 600.5 bits (1547), Expect = 1.9e-168
Identity = 288/341 (84.46%), Postives = 307/341 (90.03%), Query Frame = 1


Query: 13  ENTTMT-DSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEK 72
           ENTTM  DSNPPLF +YE LLQS A LQ  NNGN   NSVMEECQLPLIDL GL+SS+EK
Sbjct: 4   ENTTMMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSDEK 63

Query: 73  ERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYR 132
           ER AC REIF AS EWGFFQVINHGI TELL+RM+KEQ+KLFGVPFEKK TSGILD+SYR
Sbjct: 64  ERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSYR 123

Query: 133 WGTPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLV 192
           WGTPTA HPNQFSWSEAFHIPLTKVSEAACYG+FI LREVMEE+A+AMSK+AR LAGVLV
Sbjct: 124 WGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLV 183

Query: 193 ECLGHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQL 252
           E +G RKE+ +DICDESTCFLRLNHYPICPF GEVSGLVPHTDSDFLTILHQD  GGLQ+
Sbjct: 184 ESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQV 243

Query: 253 MKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHS 312
           MKGSQW+AVKPNP+ALVVNIGDLLQ WSNDTYKS KHRVV N MRERYSIAYFLCPSYHS
Sbjct: 244 MKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHRVVANPMRERYSIAYFLCPSYHS 303

Query: 313 PIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRL 353
            IGSCR+PSPYRHFTFAEYRKQVRDDVEK GYK+GLS FRL
Sbjct: 304 QIGSCREPSPYRHFTFAEYRKQVRDDVEKTGYKIGLSNFRL 344

[Close]

BLAST of Cp4.1LG01g07810 vs. NCBI nr
Match: gi|703159524|ref|XP_010112271.1| (Gibberellin 2-beta-dioxygenase 8 [Morus notabilis])

HSP 1 Score: 463.4 bits (1191), Expect = 3.7e-127
Identity = 223/341 (65.40%), Postives = 264/341 (77.42%), Query Frame = 1


Query: 19  DSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRACSR 78
           DSNPPL   Y+ LL   + LQ             EECQLP+IDL GL+S    ER  C++
Sbjct: 4   DSNPPLLDHYKALLDHSSTLQTAECDGHFDGVAAEECQLPMIDLNGLKSDEALERVECAK 63

Query: 79  EIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGTPTAA 138
            I  A++EWGFFQV+NHGI  EL+ +M KEQ KLF  PFE K+TS +L++SYRWGTPTA 
Sbjct: 64  AISKAASEWGFFQVVNHGISLELVKKMRKEQTKLFEAPFEDKATSRLLNNSYRWGTPTAT 123

Query: 139 HPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGHRK 198
            P+QFSWSEAFHIPLTKVSE  CYG+FI+LRE  EE A AMSKLARSLAGVL E LGHR+
Sbjct: 124 RPSQFSWSEAFHIPLTKVSEEDCYGDFISLREPTEEFAAAMSKLARSLAGVLAENLGHRR 183

Query: 199 EIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQWV 258
           ++F++ICDESTCFLRLN YP C    E+ GLVPHTDS FLTIL QD  GGLQLMK  +WV
Sbjct: 184 QVFEEICDESTCFLRLNRYPACAVSSEIFGLVPHTDSGFLTILRQDHVGGLQLMKDYKWV 243

Query: 259 AVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSPIGSCRQ 318
           +VKPNPEAL+VNIGDL Q WSND +KSV+HRV+TN M ERYSIAYFLCPSY S IGSCR+
Sbjct: 244 SVKPNPEALIVNIGDLFQAWSNDVFKSVEHRVMTNKM-ERYSIAYFLCPSYDSLIGSCRE 303

Query: 319 PSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRLVNINTNS 360
           PS YR FTF +YR QV++DV+K+GYKVGLS+F L + +T++
Sbjct: 304 PSVYRKFTFRQYRSQVQEDVKKMGYKVGLSRFLLQDESTHT 343

[Close]

BLAST of Cp4.1LG01g07810 vs. NCBI nr
Match: gi|297738275|emb|CBI27476.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 461.1 bits (1185), Expect = 1.8e-126
Identity = 223/339 (65.78%), Postives = 267/339 (78.76%), Query Frame = 1


Query: 16  TMTDSNPPLFHRYELLLQ--SKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKER 75
           TMT+SNPPL   Y +L Q      +Q  + GN    + MEECQLPLIDL GL S +E+ER
Sbjct: 50  TMTNSNPPLLQHYGVLCQHGETPAVQQRDCGNN--GAAMEECQLPLIDLEGLWSESEEER 109

Query: 76  RACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWG 135
            AC+  I  AS++WGFFQV+NHGIR ELLS M +EQ+KLF  PFE+K+   +LD+SYRWG
Sbjct: 110 LACASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWG 169

Query: 136 TPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVEC 195
           TPTA  P + SWSEAFHIPLTKVSE ACYGEF +LREVM+E A AMS LAR LAGVL E 
Sbjct: 170 TPTATCPKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAES 229

Query: 196 LGHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLMK 255
           LGH+K +FD+ICDE+TCFLRLN YP CP   EV GLVPHTDSDFLTIL+QD  GGLQLMK
Sbjct: 230 LGHQKGVFDEICDENTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMK 289

Query: 256 GSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSPI 315
            S+WVAVKPN + L+VNIGDL Q WSN+ YKSV+H+V+ N+++ERYSIAYFLCPSY + I
Sbjct: 290 DSKWVAVKPNKDTLIVNIGDLFQAWSNNEYKSVEHQVMANAIKERYSIAYFLCPSYDAFI 349

Query: 316 GSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRL 353
           GSC +P+ YR FTF EYR+QV++DV+K G+KVGL +F L
Sbjct: 350 GSCSEPTIYRKFTFGEYRQQVQEDVKKTGHKVGLPRFLL 386

[Close]

BLAST of Cp4.1LG01g07810 vs. NCBI nr
Match: gi|359473517|ref|XP_003631313.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Vitis vinifera])

HSP 1 Score: 459.5 bits (1181), Expect = 5.3e-126
Identity = 222/338 (65.68%), Postives = 266/338 (78.70%), Query Frame = 1


Query: 17  MTDSNPPLFHRYELLLQ--SKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERR 76
           MT+SNPPL   Y +L Q      +Q  + GN    + MEECQLPLIDL GL S +E+ER 
Sbjct: 1   MTNSNPPLLQHYGVLCQHGETPAVQQRDCGNN--GAAMEECQLPLIDLEGLWSESEEERL 60

Query: 77  ACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGT 136
           AC+  I  AS++WGFFQV+NHGIR ELLS M +EQ+KLF  PFE+K+   +LD+SYRWGT
Sbjct: 61  ACASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWGT 120

Query: 137 PTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECL 196
           PTA  P + SWSEAFHIPLTKVSE ACYGEF +LREVM+E A AMS LAR LAGVL E L
Sbjct: 121 PTATCPKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAESL 180

Query: 197 GHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLMKG 256
           GH+K +FD+ICDE+TCFLRLN YP CP   EV GLVPHTDSDFLTIL+QD  GGLQLMK 
Sbjct: 181 GHQKGVFDEICDENTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMKD 240

Query: 257 SQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSPIG 316
           S+WVAVKPN + L+VNIGDL Q WSN+ YKSV+H+V+ N+++ERYSIAYFLCPSY + IG
Sbjct: 241 SKWVAVKPNKDTLIVNIGDLFQAWSNNEYKSVEHQVMANAIKERYSIAYFLCPSYDAFIG 300

Query: 317 SCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRL 353
           SC +P+ YR FTF EYR+QV++DV+K G+KVGL +F L
Sbjct: 301 SCSEPTIYRKFTFGEYRQQVQEDVKKTGHKVGLPRFLL 336

[Close]

BLAST of Cp4.1LG01g07810 vs. NCBI nr
Match: gi|147779231|emb|CAN72288.1| (hypothetical protein VITISV_025459 [Vitis vinifera])

HSP 1 Score: 457.2 bits (1175), Expect = 2.6e-125
Identity = 222/338 (65.68%), Postives = 264/338 (78.11%), Query Frame = 1


Query: 17  MTDSNPPLFHRYELLLQ--SKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERR 76
           MT+SNPPL   Y +L Q      +Q  + GN    + MEECQLPLIDL GL S +E+ER 
Sbjct: 1   MTNSNPPLLQHYGVLCQHGETPAVQQRDCGNN--GAAMEECQLPLIDLEGLWSESEEERL 60

Query: 77  ACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGT 136
           AC+  I  AS++WGFFQV+NHGIR ELLS M +EQ+KLF  PFE+K+   +LD+SYRWGT
Sbjct: 61  ACASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWGT 120

Query: 137 PTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECL 196
           PTA    + SWSEAFHIPLTKVSE ACYGEF +LREVM+E A AMS LAR LAGVL E L
Sbjct: 121 PTATRLKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAESL 180

Query: 197 GHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLMKG 256
           GH+K +FD+ICDESTCFLRLN YP CP   EV GLVPHTDSDFLTIL+QD  GGLQLMK 
Sbjct: 181 GHQKGVFDEICDESTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMKD 240

Query: 257 SQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSPIG 316
           S+WVAVKPN + L+VNIGDL Q WSN+ YKSV+H+V+ N+++ERYSIAYFLCPSY + IG
Sbjct: 241 SKWVAVKPNKDTLIVNIGDLFQAWSNNEYKSVEHQVMANAIKERYSIAYFLCPSYDAFIG 300

Query: 317 SCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRL 353
           SC +P+ YR FTF EYR QV++DV+K G+KVGL +F L
Sbjct: 301 SCSEPTIYRKFTFGEYRXQVQEDVKKTGHKVGLPRFLL 336

The following BLAST results are available for this feature:

BLAST of Cp4.1LG01g07810 vs. Swiss-Prot


Match Name	E-value	Identity	Description
G2OX8_ARATH	4.3e-91	52.63	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2	[more]
G2OX7_ARATH	3.0e-68	44.63	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1	[more]
GAOX1_ARATH	4.6e-37	35.00	Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2	[more]
DLO2_ARATH	1.8e-36	33.88	Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana GN=DLO2 PE=2 SV=1	[more]
GAOX3_ARATH	1.5e-35	32.38	Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana GN=GA20OX3 PE=2 SV=1	[more]

BLAST of Cp4.1LG01g07810 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KIW0_CUCSA	1.3e-168	84.46	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G052540 PE=3 SV=1	[more]
W9SYI1_9ROSA	2.6e-127	65.40	Gibberellin 2-beta-dioxygenase 8 OS=Morus notabilis GN=L484_014953 PE=3 SV=1	[more]
F6HG07_VITVI	1.3e-126	65.78	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g01650 PE=3 SV=...	[more]
A5BF50_VITVI	1.8e-125	65.68	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_025459 PE=3 SV=1	[more]
A0A061E9S8_THECC	3.1e-125	64.45	Gibberellin 2-oxidase 8 OS=Theobroma cacao GN=TCM_011301 PE=3 SV=1	[more]

BLAST of Cp4.1LG01g07810 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G21200.1	2.4e-92	52.63	gibberellin 2-oxidase 8	[more]
AT1G50960.1	1.7e-69	44.63	gibberellin 2-oxidase 7	[more]
AT5G58660.1	1.7e-42	31.99	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT5G05600.1	7.3e-41	34.81	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT3G19010.1	1.2e-40	33.76	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]

BLAST of Cp4.1LG01g07810 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778698231\|ref\|XP_004142323.2\|	1.9e-168	84.46	PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus]	[more]
gi\|703159524\|ref\|XP_010112271.1\|	3.7e-127	65.40	Gibberellin 2-beta-dioxygenase 8 [Morus notabilis]	[more]
gi\|297738275\|emb\|CBI27476.3\|	1.8e-126	65.78	unnamed protein product [Vitis vinifera]	[more]
gi\|359473517\|ref\|XP_003631313.1\|	5.3e-126	65.68	PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Vitis vinifera]	[more]
gi\|147779231\|emb\|CAN72288.1\|	2.6e-125	65.68	hypothetical protein VITISV_025459 [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR027443	IPNS-like
IPR026992	DIOX_N
IPR005123	Oxoglu/Fe-dep_dioxygenase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0043547	positive regulation of GTPase activity
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0005096	GTPase activator activity
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0051213	dioxygenase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG01g07810.1	Cp4.1LG01g07810.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 211..307 score: 1.5
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROFILE	PS51471	FE2OG_OXY	coord: 206..307 score: 13
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 57..152 score: 7.4
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 48..334 score: 2.1
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 10..345 score: 4.3E
None	No IPR available	PANTHER	PTHR10209:SF177	SUBFAMILY NOT NAMED	coord: 10..345 score: 4.3E
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 49..334 score: 6.32

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG01g07810	CmaCh04G009860	Cucurbita maxima (Rimu)	cmacpeB720
Cp4.1LG01g07810	CmoCh04G010620	Cucurbita moschata (Rifu)	cmocpeB673
Cp4.1LG01g07810	Csa5G052540	Cucumber (Chinese Long) v2	cpecuB428
Cp4.1LG01g07810	ClCG07G007000	Watermelon (Charleston Gray)	cpewcgB409
Cp4.1LG01g07810	CSPI05G01270	Wild cucumber (PI 183967)	cpecpiB433
Cp4.1LG01g07810	Lsi04G011840	Bottle gourd (USVL1VR-Ls)	cpelsiB361
Cp4.1LG01g07810	MELO3C034260.2	Melon (DHL92) v3.6.1	cpemedB444
Cp4.1LG01g07810	CsaV3_5G015180	Cucumber (Chinese Long) v3	cpecucB0533
Cp4.1LG01g07810	Bhi07G000219	Wax gourd	cpewgoB0553
Cp4.1LG01g07810	CsGy5G011470	Cucumber (Gy14) v2	cgybcpeB638
Cp4.1LG01g07810	Carg00099	Silver-seed gourd	carcpeB0938
Cp4.1LG01g07810	Carg21988	Silver-seed gourd	carcpeB0259

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG01g07810	Cucurbita pepo (Zucchini)	cpecpeB374
Cp4.1LG01g07810	Cucurbita maxima (Rimu)	cmacpeB722
Cp4.1LG01g07810	Cucurbita moschata (Rifu)	cmocpeB674
Cp4.1LG01g07810	Wild cucumber (PI 183967)	cpecpiB436
Cp4.1LG01g07810	Cucumber (Chinese Long) v2	cpecuB429
Cp4.1LG01g07810	Melon (DHL92) v3.5.1	cpemeB383