Lsi04G011840 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi04G011840
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Gibberellin 2-beta-dioxygenase 8
Location	chr04 : 17534239 .. 17537792 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGAGAGAATCAATATCTTCGCGACAATTGTCACTACATGGTAGCTCCTTTACAATGTCCTCTTCTGTCCTCTTTGTTTGCTTCCACTTGTGATTTTCCTCCTCCCCACAATTTAAACCCAACTTTTCTTTACCACAAACAGGGTATATAAAGAACACTTCTCAGTTTCAAAACTTTTGTAGGAAACTCACATGTTTAAGGAAGATACTACTATGATGAACTCTAATCCACCACTCTTCCATCAATATGAGTTCCTTTTGCAATCTACAGCTCATTTACAACCATTTAACAATGGCAATCAACCAACTAACTCTGTCATGGAGGAATGTCAGCTTCCACTTATTGATCTTAAAGGTTTGAGAAGTAGCAATGAAAAGGAGAGGGTGGCTTGCAGAAGAGAGATTTTTGAAGCATCGGAAGAATGGGGATTCTTCCAAGTGATAAACCATGGGATACGCACAGAGCTGCTGAACAGAATGAACAAAGATCAAATGAAGTTGTTTGGGGTACCCTTTGAGAAAAAATTTACGAGTGGGATTTTGGATAATTCTTACAGATGGGGAACACCTACTGCAACCCACCCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGCTTGCTATGGCGAGTTCATTTACTTAAGGTATCAATTTTATCAACCTTCATCCGGCCACATACTTCTCTTAAGCATTGTTTTTATTTCATGTAGTTCCAATACTTAAAATATGGTGTCTATATCAGACTATATGGTCTTTTAGACTTGAAAAAATTTTAGATTAATTCCAGATTATGCTTTTATTTGTTAATAATATCACGAAGGCTGATATGGCATGCTTGTTAATTTATCCAACCCTTTGCCATACCATGCCAACTCAGCCAGAAGTTATCTAATAATCATGTCACATTCGTATTTTAGATCAAATTTTAGGAACATAATTGAATTTTCTTTAAAGTTCTATTTATAATCTAACCTGATATCTTCTATAATATAATTGTTCATGCACTTCTCATCAACATCTCGCGAACTTGAAGAGTTTAGCTCTTCTAGACATTTTCACAAACAGTTAGACTGCAAAAAGTAATGGCAATTCACTAGCATCAAATGGCCACATTCTTCCACAATTGTACTAAATTTGAAAAATAAGGAGAACCCATTAGTGAACTAACTGACCTTACATCTTCGAACGGCAGGGAAGTGATGGAGGAACTGGCAAGCGCGATGTCGAAGCTAGCAAAATCATTGGCAGGAGTTTTAGTAGAAAGTTTGGGGCACCGGAAGGAATTATTGGACGATATTTGCGACGAAAGCACGTGCTTCCTCCGGTTGAATCACTACCCGATCTGTCCATTTTCCGGGGAAGTTTCCGGGCTGGTGCCCCACACGGACAGCGATTTTCTTACCGTACTTCATCAAGATTGCGGCGGCGGGCTTCAGCTGATGAAAGGATCGCAATGGGTAGCTGTGAAACCCAACCCAGAAGCCCTTGTTGTCAACATTGGAGATCTTCTTCAGGTACACACTCCAAATTCTTTTGCTTTAGTTTGCCTGTGGGAATGGAAAATACATAATTTTGGTCTTTGTGGTAAGGTTAGAATTTCCTAAGATCTTAAAATTTAGATTTTTAGTTTTTATCCATCCATCAAATTTCATAAACAGTCTCTATGCGTAAATAGTCTTTGATAGGGGTGAAATTCGTACTTTAGATTACTACAAATATCAATTTATATCCCTAAATTTTAAAAGTTGTATTAATTTAAACCATAAACTATGAATTGTATTAATTTAAACACTAAACTTAGTGTATCAATTTACACCCTCTAATATATTTCGTTTGAAAAAACTTCGTGTAAAACTTATAATTATATCAATTAAACTTTTAAGCATTCATAAATGAATCAATTTAGACTTTTAGTTAGAAATTCATATGAGAATAGTCTATGCATTTATTTTAATCGTTCATTTTGTAAAATAGACATTTGAAGAAGTTTAAATATGTTTGAAAATTTATGCATGGATAATTTTCAAAAGTAATCGTAATGAAATGTCTAAACTAATTGGCTTACGAAAATTTTGGAGTTTAATAGACTGAAATTATAAATTTCACACATTATTGGTTGAACAAAATTTAGAGGAGGGGAATGTGAAAAGCTTCAGACCAAGAGAAACTTATAATTAGTTATGGAAATTAGTTTTGAGAGACTAAATTTAAGATTAATTAAAAGTACAGAGACTAATTTCAAAATATAAAGCTATAAATTTCCAACCTTTACTTTACTTTACCTTTGAATCCATCGTTAAATATCAAAATACAAAATTATACTTACCATATCAAAATTTCGAAATTTGAAGTAGTTCATCTAATCAAGAGTAATGAAAACATCTTTAGAAAAATAATTTATGCTTCAACTAATTAAACTGTCGGCACAAATTTGTGGAAGTTCAGTCCCATTATTATTCTTTTTATTTAATTTGTTTGTTATTTGAAAAAGACATATCATTATGCCTCAAATTATATACCAATTTTCAATTTCATTTTTAATGTTTCAAAAGTTTTATTTTGTCCCTAGTGTTGTCAAACCTACTAATTTTTTTTTAAGTCTAATGGTACAGTACGTCACCAAATGGATTTTCCAATTAAATAAGAGTTGATTTAGGGAGGTTGGTCATCAACTTCTACAATTATATCATAAAAATACATTGGTAGAGGGGAAATTATAATCAAATAACAATATTAGTTTTAAAACATTCCCTTAACATTCTCTCTCTCTCACTCAATATAAATAAAAAGTTAGACTTTTAACATTTTTAAGTAACTAGTTGCAAATATAGCAATCATGCCTAAAAGTATTAGTAAATATAACACAATGCAAAAGAATTTGCAAATATAGCAAAATTTAGATCCAGCTTTCATAGTCTATTAGTGATAGACCATATCGCTAATAAAAGTTTATTAGCAATAAAATCTATCACATATAGATTTTGTTATATTTGCAACTCTTTAACAATGTTGTTATACACTTAATTATTAATCGGTAGCAATTACCCTAACATGCATGTTCTTCTACTTTTGATAATGATTTAGTCCAATAATTTGATACCTAATATTAAAGTCATTGTAATCAATAGACAAATAATTAAAGTGTTAAACCACCTTTCAATTTTGGACTATAGGTCTGCTTACTTCACTCATTTTCAATATTCCCTTTATAAGGTGGTTTTTTCATAAAATATTTGGGTGAAAATGTTATCTCAACAACTCAATCAACTACATTTCTCAAAGTACATGCAACATATCATTACAAATAACAGAAAAAAAGTATGTTGTCATGCCTACAGTTTTAGAATTATTCTGTGTTTTTTTTTATTATCTTTCATTTTGCATATCTTTGCCTCCCTCCTATGGAACTTCTTGATCCAAACATGTGTGGTATCTCCTCATTATAACCTTCAACAGATTCCCTTGGTTTGTCCACCTGGGTTGGATCCACTGGAACAGAACCTTGATCTAAGCCCTATTGGAACATAA

mRNA sequence

GAGAGAGAATCAATATCTTCGCGACAATTGTCACTACATGGTAGCTCCTTTACAATGTCCTCTTCTGTCCTCTTTGTTTGCTTCCACTTGTGATTTTCCTCCTCCCCACAATTTAAACCCAACTTTTCTTTACCACAAACAGGGTATATAAAGAACACTTCTCAGTTTCAAAACTTTTGTAGGAAACTCACATGTTTAAGGAAGATACTACTATGATGAACTCTAATCCACCACTCTTCCATCAATATGAGTTCCTTTTGCAATCTACAGCTCATTTACAACCATTTAACAATGGCAATCAACCAACTAACTCTGTCATGGAGGAATGTCAGCTTCCACTTATTGATCTTAAAGGTTTGAGAAGTAGCAATGAAAAGGAGAGGGTGGCTTGCAGAAGAGAGATTTTTGAAGCATCGGAAGAATGGGGATTCTTCCAAGTGATAAACCATGGGATACGCACAGAGCTGCTGAACAGAATGAACAAAGATCAAATGAAGTTGTTTGGGGTACCCTTTGAGAAAAAATTTACGAGTGGGATTTTGGATAATTCTTACAGATGGGGAACACCTACTGCAACCCACCCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGCTTGCTATGGCGAGTTCATTTACTTAAGGGAAGTGATGGAGGAACTGGCAAGCGCGATGTCGAAGCTAGCAAAATCATTGGCAGGAGTTTTAGTAGAAAGTTTGGGGCACCGGAAGGAATTATTGGACGATATTTGCGACGAAAGCACGTGCTTCCTCCGGTTGAATCACTACCCGATCTGTCCATTTTCCGGGGAAGTTTCCGGGCTGGTGCCCCACACGGACAGCGATTTTCTTACCGTACTTCATCAAGATTGCGGCGGCGGGCTTCAGCTGATGAAAGGATCGCAATGGGTAGCTGTGAAACCCAACCCAGAAGCCCTTGTTGTCAACATTGGAGATCTTCTTCAGGTACACACTCCAAATTCTTTTGCTTTAGTTTGCCTGTGGGAATGGAAAATACATAATTTTGGTCTTTGTGGTAAGATTCCCTTGGTTTGTCCACCTGGGTTGGATCCACTGGAACAGAACCTTGATCTAAGCCCTATTGGAACATAA

Coding sequence (CDS)

ATGTTTAAGGAAGATACTACTATGATGAACTCTAATCCACCACTCTTCCATCAATATGAGTTCCTTTTGCAATCTACAGCTCATTTACAACCATTTAACAATGGCAATCAACCAACTAACTCTGTCATGGAGGAATGTCAGCTTCCACTTATTGATCTTAAAGGTTTGAGAAGTAGCAATGAAAAGGAGAGGGTGGCTTGCAGAAGAGAGATTTTTGAAGCATCGGAAGAATGGGGATTCTTCCAAGTGATAAACCATGGGATACGCACAGAGCTGCTGAACAGAATGAACAAAGATCAAATGAAGTTGTTTGGGGTACCCTTTGAGAAAAAATTTACGAGTGGGATTTTGGATAATTCTTACAGATGGGGAACACCTACTGCAACCCACCCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGCTTGCTATGGCGAGTTCATTTACTTAAGGGAAGTGATGGAGGAACTGGCAAGCGCGATGTCGAAGCTAGCAAAATCATTGGCAGGAGTTTTAGTAGAAAGTTTGGGGCACCGGAAGGAATTATTGGACGATATTTGCGACGAAAGCACGTGCTTCCTCCGGTTGAATCACTACCCGATCTGTCCATTTTCCGGGGAAGTTTCCGGGCTGGTGCCCCACACGGACAGCGATTTTCTTACCGTACTTCATCAAGATTGCGGCGGCGGGCTTCAGCTGATGAAAGGATCGCAATGGGTAGCTGTGAAACCCAACCCAGAAGCCCTTGTTGTCAACATTGGAGATCTTCTTCAGGTACACACTCCAAATTCTTTTGCTTTAGTTTGCCTGTGGGAATGGAAAATACATAATTTTGGTCTTTGTGGTAAGATTCCCTTGGTTTGTCCACCTGGGTTGGATCCACTGGAACAGAACCTTGATCTAAGCCCTATTGGAACATAA

Protein sequence

MFKEDTTMMNSNPPLFHQYEFLLQSTAHLQPFNNGNQPTNSVMEECQLPLIDLKGLRSSNEKERVACRREIFEASEEWGFFQVINHGIRTELLNRMNKDQMKLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVESLGHRKELLDDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTVLHQDCGGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVHTPNSFALVCLWEWKIHNFGLCGKIPLVCPPGLDPLEQNLDLSPIGT

Homology

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BLAST of Lsi04G011840 vs. Swiss-Prot
Match: G2OX8_ARATH (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 242.3 bits (617), Expect = 7.3e-63
Identity = 117/228 (51.32%), Postives = 154/228 (67.54%), Query Frame = 1


Query: 41  SVMEECQLPLIDLKGLRSSNEKERVACRREIFEASEEWGFFQVINHGIRTELLNRMNKDQ 100
           +V+EE +LP+ID+  L    E+ER  C+  I  AS EWGFFQVINHGI  ++L +M ++Q
Sbjct: 35  AVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQ 94

Query: 101 MKLFGVPFEKKFTSGILD-NSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYL 160
           +++F  PF+KK  S      SYRWGTP+AT   Q SWSEAFH+P+T +S+     +F  L
Sbjct: 95  IRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFTTL 154

Query: 161 REVMEELASAMSKLAKSLAGVLVESLGHRKELLDDICDESTCFLRLNHYPICPFSGEVSG 220
              ME+ AS    LA  LA VL E  G       + C  +TC+LR+N YP CP   EV G
Sbjct: 155 SSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYG 214

Query: 221 LVPHTDSDFLTVLHQDCGGGLQLMKGSQWVAVKPNPEALVVNIGDLLQ 268
           L+PHTDSDFLT+L+QD  GGLQL+K ++W+AVKPNP+AL++NIGDL Q
Sbjct: 215 LMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQ 259

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BLAST of Lsi04G011840 vs. Swiss-Prot
Match: G2OX7_ARATH (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 4.7e-46
Identity = 102/262 (38.93%), Postives = 147/262 (56.11%), Query Frame = 1


Query: 9   MNSNPPLFHQYEFLLQSTAHLQPFNNGNQPTNSVMEECQLPLIDLKGLRSSNEKERVACR 68
           M S PP    +  +  S+       +    +       +LP+IDL  L S  E +R  C 
Sbjct: 1   MASQPPFKTNFCSIFGSSFPNSTSESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCV 60

Query: 69  REIFEASEEWGFFQVINHGIRTELLNRMNKDQMKLFGVPFEKKFTSGILD---NSYRWGT 128
           +++  A++EWGFFQ++NHGI  ++   M  ++ KLF  PF  K      D   NSYRWG 
Sbjct: 61  KQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQPFSVKVRERFSDLSKNSYRWGN 120

Query: 129 PTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVESL 188
           P+AT P Q+S SEAFHI L++VS  +   +   LR ++E     ++++A+ +  +L + +
Sbjct: 121 PSATSPAQYSVSEAFHIILSEVSRIS--DDRNNLRTIVETYVQEIARVAQMICEILGKQV 180

Query: 189 GHRKELLDDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTVLHQDCGGGLQLMKG 248
               E  ++I +    FLRLN Y    F  EV GLVPHTD+ FLT+L QD  GGL+L   
Sbjct: 181 NVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENN 240

Query: 249 SQWVAVKPNPEALVVNIGDLLQ 268
            QW++VKP  EAL VNIGD+ Q
Sbjct: 241 GQWISVKPCLEALTVNIGDMFQ 260

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BLAST of Lsi04G011840 vs. Swiss-Prot
Match: GAO1B_WHEAT (Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 6.4e-27
Identity = 90/291 (30.93%), Postives = 132/291 (45.36%), Query Frame = 1


Query: 35  GNQPTNSVMEECQLPLIDLKGLRSSNEKERVACRREIFEASEEWGFFQVINHGIRTELLN 94
           G  PT    EE  +PLID+ G+ S + +      R + EA E  GFFQV+NHGI  ELL 
Sbjct: 26  GESPTPDAAEELHVPLIDIGGMLSGDPRATAEVTRLVGEACERHGFFQVVNHGIDAELLA 85

Query: 95  RMNKDQMKLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKV------- 154
             ++     F +P  +K        + R    +  + + F+   A  +P  +        
Sbjct: 86  DAHRCVDAFFTMPLPEK------QRALRRPGESCGYASSFTGRFASKLPWKETLSFRSCP 145

Query: 155 SEAACYGEFIY---------LREVMEELASAMSKLAKSLAGVLVESLGHRKELLDDICDE 214
           S+ A   ++I          L EV     S MS+L+  +  VL ESLG  +       + 
Sbjct: 146 SDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAHYRRFFEG 205

Query: 215 STCFLRLNHYPICPFSGEVSGLVPHTDSDFLTVLHQDCGGGLQLMKGSQWVAVKPNPEAL 274
           +   +RLN+YP C    E  G  PH D   LT+LHQD  GGLQ+    +W +++P  +A 
Sbjct: 206 NDSIMRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPRADAF 265

Query: 275 VVNIGDLLQVHTPNSFALVCLWEWKIHN--------FGLCGKI-PLVCPPG 301
           VVNIGD       N     CL    +++        F LC ++  +V PPG
Sbjct: 266 VVNIGDTFMA-LSNGRYKSCLHRAVVNSKVPRKSLAFFLCPEMDKVVAPPG 309

[Close]

BLAST of Lsi04G011840 vs. Swiss-Prot
Match: GAO1D_WHEAT (Gibberellin 20 oxidase 1-D OS=Triticum aestivum GN=GA20ox1D PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.1e-26
Identity = 90/291 (30.93%), Postives = 131/291 (45.02%), Query Frame = 1


Query: 35  GNQPTNSVMEECQLPLIDLKGLRSSNEKERVACRREIFEASEEWGFFQVINHGIRTELLN 94
           G  PT    EE  +PLID+ G+ S +        R + EA E  GFFQV+NHGI  ELL 
Sbjct: 26  GESPTPDAAEELHVPLIDIGGMLSGDPAAAAEVTRLVGEACERHGFFQVVNHGIDAELLA 85

Query: 95  RMNKDQMKLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKV------- 154
             ++     F +P  +K        + R    +  + + F+   A  +P  +        
Sbjct: 86  DAHRCVDNFFTMPLPEK------QRALRHPGESCGYASSFTGRFASKLPWKETLSFRSCP 145

Query: 155 SEAACYGEFIY---------LREVMEELASAMSKLAKSLAGVLVESLGHRKELLDDICDE 214
           S+ A   ++I          L EV     S MS+L+  +  VL ESLG  +       + 
Sbjct: 146 SDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAHYRRFFEG 205

Query: 215 STCFLRLNHYPICPFSGEVSGLVPHTDSDFLTVLHQDCGGGLQLMKGSQWVAVKPNPEAL 274
           +   +RLN+YP C    E  G  PH D   LT+LHQD  GGLQ+    +W +++P  +A 
Sbjct: 206 NDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPRADAF 265

Query: 275 VVNIGDLLQVHTPNSFALVCLWEWKIHN--------FGLCGKI-PLVCPPG 301
           VVNIGD       N     CL    +++        F LC ++  +V PPG
Sbjct: 266 VVNIGDTFMA-LSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEMDKVVAPPG 309

[Close]

BLAST of Lsi04G011840 vs. Swiss-Prot
Match: ACCH3_SOLLC (1-aminocyclopropane-1-carboxylate oxidase homolog OS=Solanum lycopersicum GN=ACO3 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.4e-26
Identity = 84/278 (30.22%), Postives = 132/278 (47.48%), Query Frame = 1


Query: 49  PLIDLKGLRSSNEKERVACRREIFEASEEWGFFQVINHGIRTELLNRMNKDQMKLFGVPF 108
           P+IDL+G+     K +    + + +ASE+WGFFQV+NHGI T +L+R  +   + F    
Sbjct: 59  PVIDLQGIDEDPIKHKEIVDK-VRDASEKWGFFQVVNHGIPTSVLDRTLQGTRQFFEQDN 118

Query: 109 EKK-------------FTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGE 168
           E K             +TS +  + Y+   P A+  +      A + P  +     C   
Sbjct: 119 EVKKQYYTRDTAKKVVYTSNL--DLYKSSVPAASWRDTIFCYMAPNPPSLQEFPTPC--- 178

Query: 169 FIYLREVMEELASAMSKLAKSLAGVLVESLGHRKELLDDICDESTCFLRLNHYPICPFSG 228
                E + + +  + KL  +L  +L E LG  +  L D  D    F   N+YP CP   
Sbjct: 179 ----GESLIDFSKDVKKLGFTLLELLSEGLGLDRSYLKDYMDCFHLFCSCNYYPPCPQPE 238

Query: 229 EVSGLVPHTDSDFLTVLHQDCGGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVHTPNSFA 288
              G + HTD  F+T+L QD  GGLQ++  + WV V P P +LVVNIGD LQ+ + + + 
Sbjct: 239 LTMGTIQHTDIGFVTILLQDDMGGLQVLHQNHWVDVPPTPGSLVVNIGDFLQLLSNDKYL 298

Query: 289 LVCLWEWKIHNFGLCGKIPLVCPPGLDPLEQNLDLSPI 314
            V   E +  +  +  ++ + C  G  P + +    PI
Sbjct: 299 SV---EHRAISNNVGSRMSITCFFGESPYQSSKLYGPI 323

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BLAST of Lsi04G011840 vs. TrEMBL
Match: A0A0A0KIW0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G052540 PE=3 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 2.9e-143
Identity = 245/275 (89.09%), Postives = 265/275 (96.36%), Query Frame = 1


Query: 1   MFKEDTTMM-NSNPPLFHQYEFLLQSTAHLQPFNNGNQPTNSVMEECQLPLIDLKGLRSS 60
           MFKE+TTMM +SNPPLF QYEFLLQSTAHLQPFNNGN PTNSVMEECQLPLIDLKGL+SS
Sbjct: 1   MFKENTTMMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSS 60

Query: 61  NEKERVACRREIFEASEEWGFFQVINHGIRTELLNRMNKDQMKLFGVPFEKKFTSGILDN 120
           +EKERVACRREIFEASEEWGFFQVINHGI TELLNRMNK+Q+KLFGVPFEKKFTSGILDN
Sbjct: 61  DEKERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDN 120

Query: 121 SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAG 180
           SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYG+FIYLREVMEE+ASAMSK+A+ LAG
Sbjct: 121 SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAG 180

Query: 181 VLVESLGHRKELLDDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTVLHQDCGGG 240
           VLVES+G RKELL+DICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLT+LHQD GGG
Sbjct: 181 VLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGG 240

Query: 241 LQLMKGSQWVAVKPNPEALVVNIGDLLQVHTPNSF 275
           LQ+MKGSQW+AVKPNP+ALVVNIGDLLQ  + +++
Sbjct: 241 LQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTY 275

[Close]

BLAST of Lsi04G011840 vs. TrEMBL
Match: F6HG07_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g01650 PE=3 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 2.2e-95
Identity = 177/273 (64.84%), Postives = 206/273 (75.46%), Query Frame = 1


Query: 7   TMMNSNPPLFHQYEFLLQ--STAHLQPFNNGNQPTNSVMEECQLPLIDLKGLRSSNEKER 66
           TM NSNPPL   Y  L Q   T  +Q  + GN    + MEECQLPLIDL+GL S +E+ER
Sbjct: 23  TMTNSNPPLLQHYGVLCQHGETPAVQQRDCGNN--GAAMEECQLPLIDLEGLWSESEEER 82

Query: 67  VACRREIFEASEEWGFFQVINHGIRTELLNRMNKDQMKLFGVPFEKKFTSGILDNSYRWG 126
           +AC   I  AS +WGFFQV+NHGIR ELL+ M ++Q+KLF  PFE+K    +LDNSYRWG
Sbjct: 83  LACASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWG 142

Query: 127 TPTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVES 186
           TPTAT P + SWSEAFHIPLTKVSE ACYGEF  LREVM+E A AMS LA+ LAGVL ES
Sbjct: 143 TPTATCPKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAES 202

Query: 187 LGHRKELLDDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTVLHQDCGGGLQLMK 246
           LGH+K + D+ICDE+TCFLRLN YP CP S EV GLVPHTDSDFLT+L+QD  GGLQLMK
Sbjct: 203 LGHQKGVFDEICDENTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMK 262

Query: 247 GSQWVAVKPNPEALVVNIGDLLQVHTPNSFALV 278
            S+WVAVKPN + L+VNIGDL Q  + N +  V
Sbjct: 263 DSKWVAVKPNKDTLIVNIGDLFQAWSNNEYKSV 293

[Close]

BLAST of Lsi04G011840 vs. TrEMBL
Match: A5BF50_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_025459 PE=3 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 2.5e-94
Identity = 176/272 (64.71%), Postives = 204/272 (75.00%), Query Frame = 1


Query: 8   MMNSNPPLFHQYEFLLQ--STAHLQPFNNGNQPTNSVMEECQLPLIDLKGLRSSNEKERV 67
           M NSNPPL   Y  L Q   T  +Q  + GN    + MEECQLPLIDL+GL S +E+ER+
Sbjct: 1   MTNSNPPLLQHYGVLCQHGETPAVQQRDCGNN--GAAMEECQLPLIDLEGLWSESEEERL 60

Query: 68  ACRREIFEASEEWGFFQVINHGIRTELLNRMNKDQMKLFGVPFEKKFTSGILDNSYRWGT 127
           AC   I  AS +WGFFQV+NHGIR ELL+ M ++Q+KLF  PFE+K    +LDNSYRWGT
Sbjct: 61  ACASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWGT 120

Query: 128 PTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVESL 187
           PTAT   + SWSEAFHIPLTKVSE ACYGEF  LREVM+E A AMS LA+ LAGVL ESL
Sbjct: 121 PTATRLKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAESL 180

Query: 188 GHRKELLDDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTVLHQDCGGGLQLMKG 247
           GH+K + D+ICDESTCFLRLN YP CP S EV GLVPHTDSDFLT+L+QD  GGLQLMK 
Sbjct: 181 GHQKGVFDEICDESTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMKD 240

Query: 248 SQWVAVKPNPEALVVNIGDLLQVHTPNSFALV 278
           S+WVAVKPN + L+VNIGDL Q  + N +  V
Sbjct: 241 SKWVAVKPNKDTLIVNIGDLFQAWSNNEYKSV 270

[Close]

BLAST of Lsi04G011840 vs. TrEMBL
Match: W9SYI1_9ROSA (Gibberellin 2-beta-dioxygenase 8 OS=Morus notabilis GN=L484_014953 PE=3 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 2.7e-93
Identity = 168/270 (62.22%), Postives = 202/270 (74.81%), Query Frame = 1


Query: 8   MMNSNPPLFHQYEFLLQSTAHLQPFNNGNQPTNSVMEECQLPLIDLKGLRSSNEKERVAC 67
           +++SNPPL   Y+ LL  ++ LQ             EECQLP+IDL GL+S    ERV C
Sbjct: 2   IIDSNPPLLDHYKALLDHSSTLQTAECDGHFDGVAAEECQLPMIDLNGLKSDEALERVEC 61

Query: 68  RREIFEASEEWGFFQVINHGIRTELLNRMNKDQMKLFGVPFEKKFTSGILDNSYRWGTPT 127
            + I +A+ EWGFFQV+NHGI  EL+ +M K+Q KLF  PFE K TS +L+NSYRWGTPT
Sbjct: 62  AKAISKAASEWGFFQVVNHGISLELVKKMRKEQTKLFEAPFEDKATSRLLNNSYRWGTPT 121

Query: 128 ATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVESLGH 187
           AT P+QFSWSEAFHIPLTKVSE  CYG+FI LRE  EE A+AMSKLA+SLAGVL E+LGH
Sbjct: 122 ATRPSQFSWSEAFHIPLTKVSEEDCYGDFISLREPTEEFAAAMSKLARSLAGVLAENLGH 181

Query: 188 RKELLDDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTVLHQDCGGGLQLMKGSQ 247
           R+++ ++ICDESTCFLRLN YP C  S E+ GLVPHTDS FLT+L QD  GGLQLMK  +
Sbjct: 182 RRQVFEEICDESTCFLRLNRYPACAVSSEIFGLVPHTDSGFLTILRQDHVGGLQLMKDYK 241

Query: 248 WVAVKPNPEALVVNIGDLLQVHTPNSFALV 278
           WV+VKPNPEAL+VNIGDL Q  + + F  V
Sbjct: 242 WVSVKPNPEALIVNIGDLFQAWSNDVFKSV 271

[Close]

BLAST of Lsi04G011840 vs. TrEMBL
Match: B9RY49_RICCO (Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_0814470 PE=3 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 4.0e-92
Identity = 171/260 (65.77%), Postives = 194/260 (74.62%), Query Frame = 1


Query: 8   MMNSNPPLFHQYEFLLQSTAHLQPFNNGNQPTNSVMEECQLPLIDLKGLRSSNEKERVAC 67
           M+ SNPPL   Y  L     H  P         S MEECQLPL+DL  L+S  EKER AC
Sbjct: 1   MVESNPPLQLSYTAL----THQIPEPLERHTPQSGMEECQLPLVDLNCLKSGAEKERSAC 60

Query: 68  RREIFEASEEWGFFQVINHGIRTELLNRMNKDQMKLFGVPFEKKFTSGILDNSYRWGTPT 127
              I  AS EWGFFQV+NHGI  ELL  M ++Q+KLF  PF +K T G+L+NSYRWGTPT
Sbjct: 61  SSAISTASSEWGFFQVVNHGISPELLKNMRREQVKLFQTPFHRKATCGLLNNSYRWGTPT 120

Query: 128 ATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVESLGH 187
           AT PNQFSWSEAFHIPLTK+SE ACYGEF  LREVM E A+AMSKLAK LAG+L E+LGH
Sbjct: 121 ATCPNQFSWSEAFHIPLTKISEQACYGEFTSLREVMMEFAAAMSKLAKLLAGILAENLGH 180

Query: 188 RKELLDDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTVLHQDCGGGLQLMKGSQ 247
            + + ++IC ES CFLRLN YP CP S E+ GLVPHTDSDFLT+L+QD  GGLQLMK S+
Sbjct: 181 PRAVFEEICHESNCFLRLNRYPSCPISPEMFGLVPHTDSDFLTILYQDEVGGLQLMKDSK 240

Query: 248 WVAVKPNPEALVVNIGDLLQ 268
           WVAVKPNPEAL+VNIGDL Q
Sbjct: 241 WVAVKPNPEALIVNIGDLFQ 256

[Close]

BLAST of Lsi04G011840 vs. TAIR10
Match: AT4G21200.1 (AT4G21200.1 gibberellin 2-oxidase 8)

HSP 1 Score: 242.3 bits (617), Expect = 4.1e-64
Identity = 117/228 (51.32%), Postives = 154/228 (67.54%), Query Frame = 1


Query: 41  SVMEECQLPLIDLKGLRSSNEKERVACRREIFEASEEWGFFQVINHGIRTELLNRMNKDQ 100
           +V+EE +LP+ID+  L    E+ER  C+  I  AS EWGFFQVINHGI  ++L +M ++Q
Sbjct: 35  AVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQ 94

Query: 101 MKLFGVPFEKKFTSGILD-NSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYL 160
           +++F  PF+KK  S      SYRWGTP+AT   Q SWSEAFH+P+T +S+     +F  L
Sbjct: 95  IRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFTTL 154

Query: 161 REVMEELASAMSKLAKSLAGVLVESLGHRKELLDDICDESTCFLRLNHYPICPFSGEVSG 220
              ME+ AS    LA  LA VL E  G       + C  +TC+LR+N YP CP   EV G
Sbjct: 155 SSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYG 214

Query: 221 LVPHTDSDFLTVLHQDCGGGLQLMKGSQWVAVKPNPEALVVNIGDLLQ 268
           L+PHTDSDFLT+L+QD  GGLQL+K ++W+AVKPNP+AL++NIGDL Q
Sbjct: 215 LMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQ 259

[Close]

BLAST of Lsi04G011840 vs. TAIR10
Match: AT1G50960.1 (AT1G50960.1 gibberellin 2-oxidase 7)

HSP 1 Score: 186.4 bits (472), Expect = 2.7e-47
Identity = 102/262 (38.93%), Postives = 147/262 (56.11%), Query Frame = 1


Query: 9   MNSNPPLFHQYEFLLQSTAHLQPFNNGNQPTNSVMEECQLPLIDLKGLRSSNEKERVACR 68
           M S PP    +  +  S+       +    +       +LP+IDL  L S  E +R  C 
Sbjct: 1   MASQPPFKTNFCSIFGSSFPNSTSESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCV 60

Query: 69  REIFEASEEWGFFQVINHGIRTELLNRMNKDQMKLFGVPFEKKFTSGILD---NSYRWGT 128
           +++  A++EWGFFQ++NHGI  ++   M  ++ KLF  PF  K      D   NSYRWG 
Sbjct: 61  KQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQPFSVKVRERFSDLSKNSYRWGN 120

Query: 129 PTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVESL 188
           P+AT P Q+S SEAFHI L++VS  +   +   LR ++E     ++++A+ +  +L + +
Sbjct: 121 PSATSPAQYSVSEAFHIILSEVSRIS--DDRNNLRTIVETYVQEIARVAQMICEILGKQV 180

Query: 189 GHRKELLDDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTVLHQDCGGGLQLMKG 248
               E  ++I +    FLRLN Y    F  EV GLVPHTD+ FLT+L QD  GGL+L   
Sbjct: 181 NVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENN 240

Query: 249 SQWVAVKPNPEALVVNIGDLLQ 268
            QW++VKP  EAL VNIGD+ Q
Sbjct: 241 GQWISVKPCLEALTVNIGDMFQ 260

[Close]

BLAST of Lsi04G011840 vs. TAIR10
Match: AT4G25300.1 (AT4G25300.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 125.2 bits (313), Expect = 7.3e-29
Identity = 73/243 (30.04%), Postives = 130/243 (53.50%), Query Frame = 1


Query: 47  QLPLIDLKGLRSSNEKERVACRREIFEASEEWGFFQVINHGIRTELLNRMNKDQMKLFGV 106
           Q+P+ID+  L SS   +    + +   A +EWGFFQ++NHG+ +  LN++  +    F +
Sbjct: 51  QIPIIDMSLLCSSTSMDSEIDKLD--SACKEWGFFQLVNHGMESSFLNKVKSEVQDFFNL 110

Query: 107 PFEKKFTSGILDNSYRWGTP----------TATHPNQFSWSEAFHIPL--TKVSEAACYG 166
           P E+K           W  P            +   +  W++ F + +   ++ +   + 
Sbjct: 111 PMEEK--------KNLWQQPDEIEGFGQVFVVSEEQKLDWADMFFLTMQPVRLRKPHLFP 170

Query: 167 EF-IYLREVMEELASAMSKLAKSLAGVLVESLGHRKELLDDICDESTCF-LRLNHYPICP 226
           +  +  R+ ++  ++ +  +AK L G +  +L  + E +D + D+     +RLN+YP CP
Sbjct: 171 KLPLPFRDTLDMYSAEVKSIAKILLGKIAVALKIKPEEMDKLFDDELGQRIRLNYYPRCP 230

Query: 227 FSGEVSGLVPHTDSDFLTVLHQ-DCGGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVHTP 275
              +V GL PH+DS  LT+L Q +   GLQ+ K ++WV+VKP P ALVVN+GD+L++ T 
Sbjct: 231 EPDKVIGLTPHSDSTGLTILLQANEVEGLQIKKNAKWVSVKPLPNALVVNVGDILEIITN 283

[Close]

BLAST of Lsi04G011840 vs. TAIR10
Match: AT2G30830.1 (AT2G30830.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 122.5 bits (306), Expect = 4.7e-28
Identity = 77/236 (32.63%), Postives = 125/236 (52.97%), Query Frame = 1


Query: 47  QLPLIDLKGLRSSNEKERVACRREIFEASEEWGFFQVINHGIRTELLNRMNKDQMKLF-- 106
           ++P ID+ G    +   R +   ++  A E++GFFQVINHGI  E++  M KD ++ F  
Sbjct: 53  EIPTIDVGGGVFESTVTRKSVIAKVRAAVEKFGFFQVINHGIPLEVMESM-KDGIRGFHE 112

Query: 107 -GVPFEKKFTSGILDNSYRWGTPTATHPNQFS-WSEAFHIPLTKVSEAACYGEF-IYLRE 166
                +K F S  +    ++ T    + +Q + W +   + +    +    G+  +  RE
Sbjct: 113 QDSEVKKTFYSRDITKKVKYNTNFDLYSSQAANWRDT--LTMVMAPDVPQAGDLPVICRE 172

Query: 167 VMEELASAMSKLAKSLAGVLVESLGHRKELLDDICDESTCFLRLNHYPICPFSGEVSGLV 226
           +M E +  M KL + +  +L E+LG +   L ++    +  L  ++YP CP      G+ 
Sbjct: 173 IMLEYSKRMMKLGELIFELLSEALGLKPNHLKELNCAKSLSLLSHYYPPCPEPDRTFGIS 232

Query: 227 PHTDSDFLTVLHQDCGGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVHTPNSFALV 278
            HTD  F+T+L QD  GGLQ++    W+ V PNPEAL+VN+GDLLQ+ T + F  V
Sbjct: 233 SHTDISFITILLQDHIGGLQVLHDGYWIDVPPNPEALIVNLGDLLQLITNDKFVSV 285

[Close]

BLAST of Lsi04G011840 vs. TAIR10
Match: AT5G05600.1 (AT5G05600.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 119.4 bits (298), Expect = 4.0e-27
Identity = 78/259 (30.12%), Postives = 129/259 (49.81%), Query Frame = 1


Query: 27  AHLQPFNNGNQPTNSVMEECQLPLIDLKGLRSSNEKERVACRREIFEASEEWGFFQVINH 86
           A L+P    + PT +      +P+IDL+GL S            I EA   WGFFQV+NH
Sbjct: 46  ASLRPTTTEDAPTAT-----NIPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNH 105

Query: 87  GIRTELLNRMNKDQMKLFGVPFEKKFTSGILDNSYR-WGTPTATHPN-QFSWSEAF--HI 146
           G++ EL++   ++  + F +P   K T      +Y  +G+           WS+ +  H+
Sbjct: 106 GVKPELMDAARENWREFFHMPVNAKETYSNSPRTYEGYGSRLGVEKGASLDWSDYYFLHL 165

Query: 147 PLTKVSEAACYGEF-IYLREVMEELASAMSKLAKSLAGVLVESLGHRKELLDDIC--DES 206
               + +   +  F   +REV++E    + KL+  +  VL  +LG +++   +    +  
Sbjct: 166 LPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENI 225

Query: 207 TCFLRLNHYPICPFSGEVSGLVPHTDSDFLTV-LHQDCGGGLQLMKGSQWVAVKPNPEAL 266
              LR+N+YP CP      GL PH+D   +T+ L  D   GLQ+ K   W+ VKP+P A 
Sbjct: 226 GACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAF 285

Query: 267 VVNIGDLLQVHTPNSFALV 278
           +VNIGD +Q+ + +++  V
Sbjct: 286 IVNIGDQIQILSNSTYKSV 299

[Close]

BLAST of Lsi04G011840 vs. NCBI nr
Match: gi|659109414|ref|XP_008454707.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X2 [Cucumis melo])

HSP 1 Score: 518.8 bits (1335), Expect = 6.4e-144
Identity = 245/272 (90.07%), Postives = 261/272 (95.96%), Query Frame = 1


Query: 6   TTMMNSNPPLFHQYEFLLQSTAHLQPFNNGNQPTNSVMEECQLPLIDLKGLRSSNEKERV 65
           TTMM+SNPPLF QYEFLLQSTAHLQPFNNGN PTNSVMEECQLPLIDLKGL+SSNEKERV
Sbjct: 7   TTMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSNEKERV 66

Query: 66  ACRREIFEASEEWGFFQVINHGIRTELLNRMNKDQMKLFGVPFEKKFTSGILDNSYRWGT 125
           ACRREIFEASEEWGFFQVINHGI TELLNRMNK+Q+KLFGVPFEKK TSGILDNSYRWGT
Sbjct: 67  ACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKITSGILDNSYRWGT 126

Query: 126 PTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVESL 185
           PTATHPNQ SWSEAFHIPLTKVSEAACYG+FIYLREVMEE+ASAMSK+A+ LAGVLVES+
Sbjct: 127 PTATHPNQLSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLVESM 186

Query: 186 GHRKELLDDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTVLHQDCGGGLQLMKG 245
           G RKELL+DICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLT+LHQD GGGLQ+MKG
Sbjct: 187 GQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKG 246

Query: 246 SQWVAVKPNPEALVVNIGDLLQVHTPNSFALV 278
           SQW+AVKPNP+ALVVNIGDLLQV TP SF ++
Sbjct: 247 SQWLAVKPNPQALVVNIGDLLQVQTPISFHII 278

[Close]

BLAST of Lsi04G011840 vs. NCBI nr
Match: gi|659109410|ref|XP_008454705.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X1 [Cucumis melo])

HSP 1 Score: 518.8 bits (1335), Expect = 6.4e-144
Identity = 245/272 (90.07%), Postives = 261/272 (95.96%), Query Frame = 1


Query: 6   TTMMNSNPPLFHQYEFLLQSTAHLQPFNNGNQPTNSVMEECQLPLIDLKGLRSSNEKERV 65
           TTMM+SNPPLF QYEFLLQSTAHLQPFNNGN PTNSVMEECQLPLIDLKGL+SSNEKERV
Sbjct: 48  TTMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSNEKERV 107

Query: 66  ACRREIFEASEEWGFFQVINHGIRTELLNRMNKDQMKLFGVPFEKKFTSGILDNSYRWGT 125
           ACRREIFEASEEWGFFQVINHGI TELLNRMNK+Q+KLFGVPFEKK TSGILDNSYRWGT
Sbjct: 108 ACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKITSGILDNSYRWGT 167

Query: 126 PTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVESL 185
           PTATHPNQ SWSEAFHIPLTKVSEAACYG+FIYLREVMEE+ASAMSK+A+ LAGVLVES+
Sbjct: 168 PTATHPNQLSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLVESM 227

Query: 186 GHRKELLDDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTVLHQDCGGGLQLMKG 245
           G RKELL+DICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLT+LHQD GGGLQ+MKG
Sbjct: 228 GQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKG 287

Query: 246 SQWVAVKPNPEALVVNIGDLLQVHTPNSFALV 278
           SQW+AVKPNP+ALVVNIGDLLQV TP SF ++
Sbjct: 288 SQWLAVKPNPQALVVNIGDLLQVQTPISFHII 319

[Close]

BLAST of Lsi04G011840 vs. NCBI nr
Match: gi|778698231|ref|XP_004142323.2| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus])

HSP 1 Score: 516.2 bits (1328), Expect = 4.2e-143
Identity = 245/275 (89.09%), Postives = 265/275 (96.36%), Query Frame = 1


Query: 1   MFKEDTTMM-NSNPPLFHQYEFLLQSTAHLQPFNNGNQPTNSVMEECQLPLIDLKGLRSS 60
           MFKE+TTMM +SNPPLF QYEFLLQSTAHLQPFNNGN PTNSVMEECQLPLIDLKGL+SS
Sbjct: 1   MFKENTTMMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSS 60

Query: 61  NEKERVACRREIFEASEEWGFFQVINHGIRTELLNRMNKDQMKLFGVPFEKKFTSGILDN 120
           +EKERVACRREIFEASEEWGFFQVINHGI TELLNRMNK+Q+KLFGVPFEKKFTSGILDN
Sbjct: 61  DEKERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDN 120

Query: 121 SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAG 180
           SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYG+FIYLREVMEE+ASAMSK+A+ LAG
Sbjct: 121 SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAG 180

Query: 181 VLVESLGHRKELLDDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTVLHQDCGGG 240
           VLVES+G RKELL+DICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLT+LHQD GGG
Sbjct: 181 VLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGG 240

Query: 241 LQLMKGSQWVAVKPNPEALVVNIGDLLQVHTPNSF 275
           LQ+MKGSQW+AVKPNP+ALVVNIGDLLQ  + +++
Sbjct: 241 LQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTY 275

[Close]

BLAST of Lsi04G011840 vs. NCBI nr
Match: gi|297738275|emb|CBI27476.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 357.1 bits (915), Expect = 3.2e-95
Identity = 177/273 (64.84%), Postives = 206/273 (75.46%), Query Frame = 1


Query: 7   TMMNSNPPLFHQYEFLLQ--STAHLQPFNNGNQPTNSVMEECQLPLIDLKGLRSSNEKER 66
           TM NSNPPL   Y  L Q   T  +Q  + GN    + MEECQLPLIDL+GL S +E+ER
Sbjct: 50  TMTNSNPPLLQHYGVLCQHGETPAVQQRDCGNN--GAAMEECQLPLIDLEGLWSESEEER 109

Query: 67  VACRREIFEASEEWGFFQVINHGIRTELLNRMNKDQMKLFGVPFEKKFTSGILDNSYRWG 126
           +AC   I  AS +WGFFQV+NHGIR ELL+ M ++Q+KLF  PFE+K    +LDNSYRWG
Sbjct: 110 LACASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWG 169

Query: 127 TPTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVES 186
           TPTAT P + SWSEAFHIPLTKVSE ACYGEF  LREVM+E A AMS LA+ LAGVL ES
Sbjct: 170 TPTATCPKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAES 229

Query: 187 LGHRKELLDDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTVLHQDCGGGLQLMK 246
           LGH+K + D+ICDE+TCFLRLN YP CP S EV GLVPHTDSDFLT+L+QD  GGLQLMK
Sbjct: 230 LGHQKGVFDEICDENTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMK 289

Query: 247 GSQWVAVKPNPEALVVNIGDLLQVHTPNSFALV 278
            S+WVAVKPN + L+VNIGDL Q  + N +  V
Sbjct: 290 DSKWVAVKPNKDTLIVNIGDLFQAWSNNEYKSV 320

[Close]

BLAST of Lsi04G011840 vs. NCBI nr
Match: gi|359473517|ref|XP_003631313.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Vitis vinifera])

HSP 1 Score: 355.1 bits (910), Expect = 1.2e-94
Identity = 176/272 (64.71%), Postives = 205/272 (75.37%), Query Frame = 1


Query: 8   MMNSNPPLFHQYEFLLQ--STAHLQPFNNGNQPTNSVMEECQLPLIDLKGLRSSNEKERV 67
           M NSNPPL   Y  L Q   T  +Q  + GN    + MEECQLPLIDL+GL S +E+ER+
Sbjct: 1   MTNSNPPLLQHYGVLCQHGETPAVQQRDCGNN--GAAMEECQLPLIDLEGLWSESEEERL 60

Query: 68  ACRREIFEASEEWGFFQVINHGIRTELLNRMNKDQMKLFGVPFEKKFTSGILDNSYRWGT 127
           AC   I  AS +WGFFQV+NHGIR ELL+ M ++Q+KLF  PFE+K    +LDNSYRWGT
Sbjct: 61  ACASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWGT 120

Query: 128 PTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVESL 187
           PTAT P + SWSEAFHIPLTKVSE ACYGEF  LREVM+E A AMS LA+ LAGVL ESL
Sbjct: 121 PTATCPKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAESL 180

Query: 188 GHRKELLDDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTVLHQDCGGGLQLMKG 247
           GH+K + D+ICDE+TCFLRLN YP CP S EV GLVPHTDSDFLT+L+QD  GGLQLMK 
Sbjct: 181 GHQKGVFDEICDENTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMKD 240

Query: 248 SQWVAVKPNPEALVVNIGDLLQVHTPNSFALV 278
           S+WVAVKPN + L+VNIGDL Q  + N +  V
Sbjct: 241 SKWVAVKPNKDTLIVNIGDLFQAWSNNEYKSV 270

The following BLAST results are available for this feature:

BLAST of Lsi04G011840 vs. Swiss-Prot


Match Name	E-value	Identity	Description
G2OX8_ARATH	7.3e-63	51.32	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2	[more]
G2OX7_ARATH	4.7e-46	38.93	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1	[more]
GAO1B_WHEAT	6.4e-27	30.93	Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1	[more]
GAO1D_WHEAT	1.1e-26	30.93	Gibberellin 20 oxidase 1-D OS=Triticum aestivum GN=GA20ox1D PE=1 SV=1	[more]
ACCH3_SOLLC	2.4e-26	30.22	1-aminocyclopropane-1-carboxylate oxidase homolog OS=Solanum lycopersicum GN=ACO...	[more]

BLAST of Lsi04G011840 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KIW0_CUCSA	2.9e-143	89.09	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G052540 PE=3 SV=1	[more]
F6HG07_VITVI	2.2e-95	64.84	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g01650 PE=3 SV=...	[more]
A5BF50_VITVI	2.5e-94	64.71	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_025459 PE=3 SV=1	[more]
W9SYI1_9ROSA	2.7e-93	62.22	Gibberellin 2-beta-dioxygenase 8 OS=Morus notabilis GN=L484_014953 PE=3 SV=1	[more]
B9RY49_RICCO	4.0e-92	65.77	Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_0814470 PE=3 SV=1	[more]

BLAST of Lsi04G011840 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G21200.1	4.1e-64	51.32	gibberellin 2-oxidase 8	[more]
AT1G50960.1	2.7e-47	38.93	gibberellin 2-oxidase 7	[more]
AT4G25300.1	7.3e-29	30.04	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT2G30830.1	4.7e-28	32.63	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT5G05600.1	4.0e-27	30.12	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]

BLAST of Lsi04G011840 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659109414\|ref\|XP_008454707.1\|	6.4e-144	90.07	PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X2 [Cucumis melo]	[more]
gi\|659109410\|ref\|XP_008454705.1\|	6.4e-144	90.07	PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X1 [Cucumis melo]	[more]
gi\|778698231\|ref\|XP_004142323.2\|	4.2e-143	89.09	PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus]	[more]
gi\|297738275\|emb\|CBI27476.3\|	3.2e-95	64.84	unnamed protein product [Vitis vinifera]	[more]
gi\|359473517\|ref\|XP_003631313.1\|	1.2e-94	64.71	PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR027443	IPNS-like
IPR026992	DIOX_N
IPR005123	Oxoglu/Fe-dep_dioxygenase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G011840.1	Lsi04G011840.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 202..272 score: 5.0
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROFILE	PS51471	FE2OG_OXY	coord: 197..315 score: 8
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 48..143 score: 7.9
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 37..272 score: 9.4
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 1..267 score: 1.1E
None	No IPR available	PANTHER	PTHR10209:SF177	SUBFAMILY NOT NAMED	coord: 1..267 score: 1.1E
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 34..273 score: 2.47

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi04G011840	Csa5G052540	Cucumber (Chinese Long) v2	culsiB370
Lsi04G011840	CSPI05G01270	Wild cucumber (PI 183967)	cpilsiB374
Lsi04G011840	Cucsa.372030	Cucumber (Gy14) v1	cgylsiB602
Lsi04G011840	CmoCh04G010620	Cucurbita moschata (Rifu)	cmolsiB672
Lsi04G011840	Cla007482	Watermelon (97103) v1	lsiwmB308
Lsi04G011840	ClCG07G007000	Watermelon (Charleston Gray)	lsiwcgB296
Lsi04G011840	MELO3C034260.2	Melon (DHL92) v3.6.1	lsimedB298
Lsi04G011840	Bhi07G000219	Wax gourd	lsiwgoB386
Lsi04G011840	Cp4.1LG01g07810	Cucurbita pepo (Zucchini)	cpelsiB361
Lsi04G011840	CsGy5G011470	Cucumber (Gy14) v2	cgyblsiB339
Lsi04G011840	Carg00099	Silver-seed gourd	carlsiB610
Lsi04G011840	CsaV3_5G015180	Cucumber (Chinese Long) v3	cuclsiB395

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi04G011840	Watermelon (97103) v2	lsiwmbB297
Lsi04G011840	Cucurbita maxima (Rimu)	cmalsiB679
Lsi04G011840	Cucurbita maxima (Rimu)	cmalsiB680
Lsi04G011840	Cucurbita moschata (Rifu)	cmolsiB673
Lsi04G011840	Melon (DHL92) v3.5.1	lsimeB284