Cla007482 (gene) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla007482
Type	gene
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	1-aminocyclopropane-1-carboxylate oxidase (AHRD V1 **-- Q6PL00_9ASPA); contains Interpro domain(s) IPR005123 Oxoglutarate and iron-dependent oxygenase
Location	Chr7 : 16057072 .. 16058476 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTAAGGAAGATAATACTATGATGGACTCTAATCCACCACTCTTCCATCAATATGAGTTCCTTTTGCAATCCACAGCTCATTTACAACCATTTAACAATGGCAATGACCCAACTAACTCTGTCATGGAGGAATGTCAGCTTCCTCTTATTGATCTTAAAGGTTTGAGAAGTAGCAATGAAAAGGAGAGGGTGGCTTGCAGAAGAGAGATTTTTGAAGCATCCGAAGAATGGGGATTCTTCCAAGTGATAAACCATGGGATATGCACAGAGCTGCTGAGCAGAATGAACAAAGAACAAATGAAGTTGTTTGGGGTAGCCTTTGAGAAAAAATTCACAAGTGGGATTTTGGATAATTCTTACAGATGGGGAACACCAACTGCAACCCACCCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGCCTGCTATGGCGAATTCATTTACTTAAGGTATCAATTTTATCAACCTTCATCCAGCTACATACTTCTCTTAAGCATTGTCTTTATTTCATTTGGTTCTAATACTAAAAACATTGTGTCTATATCAGACGATATGGTCTTTTAGACTTGAAAAAGTTATAAATTAATTCCAGATTATGCTTTTAGTTGTTAATATCACATTTAAAGAAGGCAGATATGGCATGCTTGCTAATTTATCCAACCCTTTCCCATACCATGCCAACTCAGCCAGAAGTTATCTAATGATCATGTCACTTTCGTATTTTAGTTCAATTCTAGGAACATGATTGAATTTCTTTTTAAGTTCTATTTATAATCTAACCTGATATCTTCTATAATATAATTGTTCATGCACTTTTTCTTCAACATGTCGCGAACTCGAAGAGTTTAGCCCTTCTAGACATTTTCACAAACAGTTAGACCGCAAAAAGTAATGGCACTTCGATACCATAATCAACCAGCATTAAATGGCCACATTCTTCCACAATTGTACTAAACTAGAAAAATGAGGGGAGCCCATTAATGAACTGACTGACCTTACATCTTCGAACGGCAGGGAAGTGATGGAGGAACTGGCAAGCGCCATGTCGAAGCTAGCAAAATCTTTGGCAGGAGTTTTAGTGGAGAGTTTGGGGCACCGGAAGGAATTATTGGACAATATTTGCGACGAAAGCACATGCTTCCTCCGGTTGAATCACTACCCGATCTGTCCATTTTCGGGCGAAATTTCCGGACTGGTGCCGCACACGGATAGCGATTTTCTCACCATACTCCATCAAGATTGCGGCGGCGGACTTCAGCTGATGAAAGGATCGCAATGGGTAGCTGTGAAACCCAACCCAGAAGCCCTTGTTGTCAACATTGGAGATCTTCTTCAGGTACACACTCCAAACTCTTTTGCTTTAGTTTGCCGGTGA

mRNA sequence

ATGTTTAAGGAAGATAATACTATGATGGACTCTAATCCACCACTCTTCCATCAATATGAGTTCCTTTTGCAATCCACAGCTCATTTACAACCATTTAACAATGGCAATGACCCAACTAACTCTGTCATGGAGGAATGTCAGCTTCCTCTTATTGATCTTAAAGGTTTGAGAAGTAGCAATGAAAAGGAGAGGGTGGCTTGCAGAAGAGAGATTTTTGAAGCATCCGAAGAATGGGGATTCTTCCAAGTGATAAACCATGGGATATGCACAGAGCTGCTGAGCAGAATGAACAAAGAACAAATGAAGTTGTTTGGGGTAGCCTTTGAGAAAAAATTCACAAGTGGGATTTTGGATAATTCTTACAGATGGGGAACACCAACTGCAACCCACCCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGCCTGCTATGGCGAATTCATTTACTTAAGGGAAGTGATGGAGGAACTGGCAAGCGCCATGTCGAAGCTAGCAAAATCTTTGGCAGGAGTTTTAGTGGAGAGTTTGGGGCACCGGAAGGAATTATTGGACAATATTTGCGACGAAAGCACATGCTTCCTCCGGTTGAATCACTACCCGATCTGTCCATTTTCGGGCGAAATTTCCGGACTGGTGCCGCACACGGATAGCGATTTTCTCACCATACTCCATCAAGATTGCGGCGGCGGACTTCAGCTGATGAAAGGATCGCAATGGGTAGCTGTGAAACCCAACCCAGAAGCCCTTGTTGTCAACATTGGAGATCTTCTTCAGGTACACACTCCAAACTCTTTTGCTTTAGTTTGCCGGTGA

Coding sequence (CDS)

ATGTTTAAGGAAGATAATACTATGATGGACTCTAATCCACCACTCTTCCATCAATATGAGTTCCTTTTGCAATCCACAGCTCATTTACAACCATTTAACAATGGCAATGACCCAACTAACTCTGTCATGGAGGAATGTCAGCTTCCTCTTATTGATCTTAAAGGTTTGAGAAGTAGCAATGAAAAGGAGAGGGTGGCTTGCAGAAGAGAGATTTTTGAAGCATCCGAAGAATGGGGATTCTTCCAAGTGATAAACCATGGGATATGCACAGAGCTGCTGAGCAGAATGAACAAAGAACAAATGAAGTTGTTTGGGGTAGCCTTTGAGAAAAAATTCACAAGTGGGATTTTGGATAATTCTTACAGATGGGGAACACCAACTGCAACCCACCCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGCCTGCTATGGCGAATTCATTTACTTAAGGGAAGTGATGGAGGAACTGGCAAGCGCCATGTCGAAGCTAGCAAAATCTTTGGCAGGAGTTTTAGTGGAGAGTTTGGGGCACCGGAAGGAATTATTGGACAATATTTGCGACGAAAGCACATGCTTCCTCCGGTTGAATCACTACCCGATCTGTCCATTTTCGGGCGAAATTTCCGGACTGGTGCCGCACACGGATAGCGATTTTCTCACCATACTCCATCAAGATTGCGGCGGCGGACTTCAGCTGATGAAAGGATCGCAATGGGTAGCTGTGAAACCCAACCCAGAAGCCCTTGTTGTCAACATTGGAGATCTTCTTCAGGTACACACTCCAAACTCTTTTGCTTTAGTTTGCCGGTGA

Protein sequence

MFKEDNTMMDSNPPLFHQYEFLLQSTAHLQPFNNGNDPTNSVMEECQLPLIDLKGLRSSNEKERVACRREIFEASEEWGFFQVINHGICTELLSRMNKEQMKLFGVAFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVESLGHRKELLDNICDESTCFLRLNHYPICPFSGEISGLVPHTDSDFLTILHQDCGGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVHTPNSFALVCR

Homology

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BLAST of Cla007482 vs. Swiss-Prot
Match: G2OX8_ARATH (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 234.6 bits (597), Expect = 1.3e-60
Identity = 117/228 (51.32%), Postives = 150/228 (65.79%), Query Frame = 1


Query: 41  SVMEECQLPLIDLKGLRSSNEKERVACRREIFEASEEWGFFQVINHGICTELLSRMNKEQ 100
           +V+EE +LP+ID+  L    E+ER  C+  I  AS EWGFFQVINHGI  ++L +M +EQ
Sbjct: 35  AVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQ 94

Query: 101 MKLFGVAFEKKFTSGILD-NSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYL 160
           +++F   F+KK  S      SYRWGTP+AT   Q SWSEAFH+P+T +S+     +F  L
Sbjct: 95  IRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFTTL 154

Query: 161 REVMEELASAMSKLAKSLAGVLVESLGHRKELLDNICDESTCFLRLNHYPICPFSGEISG 220
              ME+ AS    LA  LA VL E  G         C  +TC+LR+N YP CP   E+ G
Sbjct: 155 SSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYG 214

Query: 221 LVPHTDSDFLTILHQDCGGGLQLMKGSQWVAVKPNPEALVVNIGDLLQ 268
           L+PHTDSDFLTIL+QD  GGLQL+K ++W+AVKPNP+AL++NIGDL Q
Sbjct: 215 LMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQ 259

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BLAST of Cla007482 vs. Swiss-Prot
Match: G2OX7_ARATH (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.0e-44
Identity = 103/262 (39.31%), Postives = 145/262 (55.34%), Query Frame = 1


Query: 9   MDSNPPLFHQYEFLLQSTAHLQPFNNGNDPTNSVMEECQLPLIDLKGLRSSNEKERVACR 68
           M S PP    +  +  S+       +  + +       +LP+IDL  L S  E +R  C 
Sbjct: 1   MASQPPFKTNFCSIFGSSFPNSTSESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCV 60

Query: 69  REIFEASEEWGFFQVINHGICTELLSRMNKEQMKLFGVAFEKKFTSGILD---NSYRWGT 128
           +++  A++EWGFFQ++NHGI  ++   M  E+ KLF   F  K      D   NSYRWG 
Sbjct: 61  KQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQPFSVKVRERFSDLSKNSYRWGN 120

Query: 129 PTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVESL 188
           P+AT P Q+S SEAFHI L++VS  +   +   LR ++E     ++++A+ +  +L + +
Sbjct: 121 PSATSPAQYSVSEAFHIILSEVSRIS--DDRNNLRTIVETYVQEIARVAQMICEILGKQV 180

Query: 189 GHRKELLDNICDESTCFLRLNHYPICPFSGEISGLVPHTDSDFLTILHQDCGGGLQLMKG 248
               E  +NI +    FLRLN Y    F  E+ GLVPHTD+ FLTIL QD  GGL+L   
Sbjct: 181 NVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENN 240

Query: 249 SQWVAVKPNPEALVVNIGDLLQ 268
            QW++VKP  EAL VNIGD+ Q
Sbjct: 241 GQWISVKPCLEALTVNIGDMFQ 260

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BLAST of Cla007482 vs. Swiss-Prot
Match: ACCO2_DORSP (1-aminocyclopropane-1-carboxylate oxidase 2 OS=Doritaenopsis sp. GN=ACO2 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 4.1e-25
Identity = 84/247 (34.01%), Postives = 123/247 (49.80%), Query Frame = 1


Query: 43  MEECQLPLIDLKGLRSSNEKERVACRREIFEASEEWGFFQVINHGICTELLSRM---NKE 102
           ME    P+I+++ L+ S     +A  R+   A E WGFF+++NHGI  EL++R+   NKE
Sbjct: 1   MESGSFPVINMELLQGSQRPAAMALLRD---ACENWGFFELLNHGISHELMNRVEAVNKE 60

Query: 103 QMKLFGVAFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAF---HIPLTKVSEAACYGEF 162
             + F     K+F S  LD+           P+   W   F   H+P + +S+     + 
Sbjct: 61  HYRRFREQRFKEFASKTLDSVEN------VDPDNLDWESTFFLRHLPTSNISQIPDLDDD 120

Query: 163 IYLREVMEELASAMSKLAKSLAGVLVESLGHRKELLDNI-CDES----TCFLRLNHYPIC 222
              R  M+E A  + KLA+ L  +L E LG  K  L  + C  S    T   ++++YP C
Sbjct: 121 C--RATMKEFARELEKLAERLLDLLCEDLGLEKGYLKRVFCGGSDGLPTFGTKVSNYPPC 180

Query: 223 PFSGEISGLVPHTDSDFLTILHQDCG-GGLQLMKGSQWVAVKPNPEALVVNIGDLLQVHT 278
           P    I GL  HTD+  + +L QD    GLQL+K  +W+ V P   ++VVNIGD L+V T
Sbjct: 181 PKPDLIKGLRAHTDAGGIILLFQDDKVSGLQLLKDREWIEVPPLRYSIVVNIGDQLEVIT 236

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BLAST of Cla007482 vs. Swiss-Prot
Match: ACCO_PRUMU (1-aminocyclopropane-1-carboxylate oxidase OS=Prunus mume GN=ACO1 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 4.1e-25
Identity = 78/236 (33.05%), Postives = 118/236 (50.00%), Query Frame = 1


Query: 49  PLIDLKGLRSSNEKERVACRREIFEASEEWGFFQVINHGICTELLSRMNKEQMKLFGVAF 108
           P+I+L+GL   N + R A   +I +A E WGFF++++HGI TE L  + +   + +    
Sbjct: 5   PIINLEGL---NGEGRKATMEKIKDACENWGFFELVSHGIPTEFLDTVERLTKEHYRQCL 64

Query: 109 EKKFTSGILDNSYRWGTPTATHPNQFSWSEAF---HIPLTKVSEAACYGEFIYLREVMEE 168
           E++F   +            T  N   W   F   H+P + +SE     +    R VM+E
Sbjct: 65  EQRFKELVASKGLE---AVKTEVNDMDWESTFYLRHLPKSNISEVPDLED--QYRNVMKE 124

Query: 169 LASAMSKLAKSLAGVLVESLGHRKELLDNI---CDESTCFLRLNHYPICPFSGEISGLVP 228
            A  + KLA+ L  +L E+LG  K  L       +  T   ++++YP CP    I GL  
Sbjct: 125 FALKLEKLAEQLLDLLCENLGLEKGYLKKAFYGTNGPTFGTKVSNYPPCPNPELIKGLRA 184

Query: 229 HTDSDFLTILHQDCG-GGLQLMKGSQWVAVKPNPEALVVNIGDLLQVHTPNSFALV 278
           HTD+  L +L QD    GLQL+K  QW+ V P   ++V+N+GD L+V T   +  V
Sbjct: 185 HTDAGGLILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVINLGDQLEVITNGKYRSV 232

[Close]

BLAST of Cla007482 vs. Swiss-Prot
Match: ACCO_DIACA (Probable 1-aminocyclopropane-1-carboxylate oxidase OS=Dianthus caryophyllus GN=ACO PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 6.9e-25
Identity = 80/239 (33.47%), Postives = 118/239 (49.37%), Query Frame = 1


Query: 49  PLIDLKGLRSSNEKERVACRREIFEASEEWGFFQVINHGICTELL---SRMNKEQMKLFG 108
           P+ID++ L + N  ER     +I +A   WGFFQV+NH +  EL+    RM KE  K F 
Sbjct: 8   PIIDMEKLNNYNGVERSLVLDQIKDACHNWGFFQVVNHSLSHELMDKVERMTKEHYKKFR 67

Query: 109 VAFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAF---HIPLTKVSEAACYGEFIYLREV 168
              E+KF   +            +  N   W   F   H P + +SE     +    R++
Sbjct: 68  ---EQKFKDMVQTKGL---VSAESQVNDIDWESTFYLRHRPTSNISEVPDLDD--QYRKL 127

Query: 169 MEELASAMSKLAKSLAGVLVESLGHRKELLDNI---CDESTCFLRLNHYPICPFSGEISG 228
           M+E A+ + +L++ L  +L E+LG  K  L N     +  T   ++++YP CP    I G
Sbjct: 128 MKEFAAQIERLSEQLLDLLCENLGLEKAYLKNAFYGANGPTFGTKVSNYPPCPKPDLIKG 187

Query: 229 LVPHTDSDFLTILHQDCG-GGLQLMKGSQWVAVKPNPEALVVNIGDLLQVHTPNSFALV 278
           L  HTD+  + +L QD    GLQL+K   WV V P   ++VVN+GD L+V T   +  V
Sbjct: 188 LRAHTDAGGIILLFQDDKVSGLQLLKDGHWVDVPPMKHSIVVNLGDQLEVITNGKYKSV 238

[Close]

BLAST of Cla007482 vs. TrEMBL
Match: A0A0A0KIW0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G052540 PE=3 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 1.7e-139
Identity = 243/275 (88.36%), Postives = 263/275 (95.64%), Query Frame = 1


Query: 1   MFKEDNTMM-DSNPPLFHQYEFLLQSTAHLQPFNNGNDPTNSVMEECQLPLIDLKGLRSS 60
           MFKE+ TMM DSNPPLF QYEFLLQSTAHLQPFNNGN PTNSVMEECQLPLIDLKGL+SS
Sbjct: 1   MFKENTTMMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSS 60

Query: 61  NEKERVACRREIFEASEEWGFFQVINHGICTELLSRMNKEQMKLFGVAFEKKFTSGILDN 120
           +EKERVACRREIFEASEEWGFFQVINHGI TELL+RMNKEQ+KLFGV FEKKFTSGILDN
Sbjct: 61  DEKERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDN 120

Query: 121 SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAG 180
           SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYG+FIYLREVMEE+ASAMSK+A+ LAG
Sbjct: 121 SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAG 180

Query: 181 VLVESLGHRKELLDNICDESTCFLRLNHYPICPFSGEISGLVPHTDSDFLTILHQDCGGG 240
           VLVES+G RKELL++ICDESTCFLRLNHYPICPFSGE+SGLVPHTDSDFLTILHQD GGG
Sbjct: 181 VLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGG 240

Query: 241 LQLMKGSQWVAVKPNPEALVVNIGDLLQVHTPNSF 275
           LQ+MKGSQW+AVKPNP+ALVVNIGDLLQ  + +++
Sbjct: 241 LQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTY 275

[Close]

BLAST of Cla007482 vs. TrEMBL
Match: F6HG07_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g01650 PE=3 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 3.5e-92
Identity = 176/273 (64.47%), Postives = 204/273 (74.73%), Query Frame = 1


Query: 7   TMMDSNPPLFHQYEFLLQ--STAHLQPFNNGNDPTNSVMEECQLPLIDLKGLRSSNEKER 66
           TM +SNPPL   Y  L Q   T  +Q  + GN+   + MEECQLPLIDL+GL S +E+ER
Sbjct: 23  TMTNSNPPLLQHYGVLCQHGETPAVQQRDCGNN--GAAMEECQLPLIDLEGLWSESEEER 82

Query: 67  VACRREIFEASEEWGFFQVINHGICTELLSRMNKEQMKLFGVAFEKKFTSGILDNSYRWG 126
           +AC   I  AS +WGFFQV+NHGI  ELLS M +EQ+KLF   FE+K    +LDNSYRWG
Sbjct: 83  LACASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWG 142

Query: 127 TPTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVES 186
           TPTAT P + SWSEAFHIPLTKVSE ACYGEF  LREVM+E A AMS LA+ LAGVL ES
Sbjct: 143 TPTATCPKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAES 202

Query: 187 LGHRKELLDNICDESTCFLRLNHYPICPFSGEISGLVPHTDSDFLTILHQDCGGGLQLMK 246
           LGH+K + D ICDE+TCFLRLN YP CP S E+ GLVPHTDSDFLTIL+QD  GGLQLMK
Sbjct: 203 LGHQKGVFDEICDENTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMK 262

Query: 247 GSQWVAVKPNPEALVVNIGDLLQVHTPNSFALV 278
            S+WVAVKPN + L+VNIGDL Q  + N +  V
Sbjct: 263 DSKWVAVKPNKDTLIVNIGDLFQAWSNNEYKSV 293

[Close]

BLAST of Cla007482 vs. TrEMBL
Match: W9SYI1_9ROSA (Gibberellin 2-beta-dioxygenase 8 OS=Morus notabilis GN=L484_014953 PE=3 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.0e-91
Identity = 171/270 (63.33%), Postives = 200/270 (74.07%), Query Frame = 1


Query: 8   MMDSNPPLFHQYEFLLQSTAHLQPFNNGNDPTNSVMEECQLPLIDLKGLRSSNEKERVAC 67
           ++DSNPPL   Y+ LL  ++ LQ             EECQLP+IDL GL+S    ERV C
Sbjct: 2   IIDSNPPLLDHYKALLDHSSTLQTAECDGHFDGVAAEECQLPMIDLNGLKSDEALERVEC 61

Query: 68  RREIFEASEEWGFFQVINHGICTELLSRMNKEQMKLFGVAFEKKFTSGILDNSYRWGTPT 127
            + I +A+ EWGFFQV+NHGI  EL+ +M KEQ KLF   FE K TS +L+NSYRWGTPT
Sbjct: 62  AKAISKAASEWGFFQVVNHGISLELVKKMRKEQTKLFEAPFEDKATSRLLNNSYRWGTPT 121

Query: 128 ATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVESLGH 187
           AT P+QFSWSEAFHIPLTKVSE  CYG+FI LRE  EE A+AMSKLA+SLAGVL E+LGH
Sbjct: 122 ATRPSQFSWSEAFHIPLTKVSEEDCYGDFISLREPTEEFAAAMSKLARSLAGVLAENLGH 181

Query: 188 RKELLDNICDESTCFLRLNHYPICPFSGEISGLVPHTDSDFLTILHQDCGGGLQLMKGSQ 247
           R+++ + ICDESTCFLRLN YP C  S EI GLVPHTDS FLTIL QD  GGLQLMK  +
Sbjct: 182 RRQVFEEICDESTCFLRLNRYPACAVSSEIFGLVPHTDSGFLTILRQDHVGGLQLMKDYK 241

Query: 248 WVAVKPNPEALVVNIGDLLQVHTPNSFALV 278
           WV+VKPNPEAL+VNIGDL Q  + + F  V
Sbjct: 242 WVSVKPNPEALIVNIGDLFQAWSNDVFKSV 271

[Close]

BLAST of Cla007482 vs. TrEMBL
Match: A5BF50_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_025459 PE=3 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 3.9e-91
Identity = 175/272 (64.34%), Postives = 202/272 (74.26%), Query Frame = 1


Query: 8   MMDSNPPLFHQYEFLLQ--STAHLQPFNNGNDPTNSVMEECQLPLIDLKGLRSSNEKERV 67
           M +SNPPL   Y  L Q   T  +Q  + GN+   + MEECQLPLIDL+GL S +E+ER+
Sbjct: 1   MTNSNPPLLQHYGVLCQHGETPAVQQRDCGNN--GAAMEECQLPLIDLEGLWSESEEERL 60

Query: 68  ACRREIFEASEEWGFFQVINHGICTELLSRMNKEQMKLFGVAFEKKFTSGILDNSYRWGT 127
           AC   I  AS +WGFFQV+NHGI  ELLS M +EQ+KLF   FE+K    +LDNSYRWGT
Sbjct: 61  ACASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWGT 120

Query: 128 PTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVESL 187
           PTAT   + SWSEAFHIPLTKVSE ACYGEF  LREVM+E A AMS LA+ LAGVL ESL
Sbjct: 121 PTATRLKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAESL 180

Query: 188 GHRKELLDNICDESTCFLRLNHYPICPFSGEISGLVPHTDSDFLTILHQDCGGGLQLMKG 247
           GH+K + D ICDESTCFLRLN YP CP S E+ GLVPHTDSDFLTIL+QD  GGLQLMK 
Sbjct: 181 GHQKGVFDEICDESTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMKD 240

Query: 248 SQWVAVKPNPEALVVNIGDLLQVHTPNSFALV 278
           S+WVAVKPN + L+VNIGDL Q  + N +  V
Sbjct: 241 SKWVAVKPNKDTLIVNIGDLFQAWSNNEYKSV 270

[Close]

BLAST of Cla007482 vs. TrEMBL
Match: A0A061E9S8_THECC (Gibberellin 2-oxidase 8 OS=Theobroma cacao GN=TCM_011301 PE=3 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 1.1e-90
Identity = 172/261 (65.90%), Postives = 199/261 (76.25%), Query Frame = 1


Query: 8   MMDSNPPLFHQYEFLL-QSTAHLQPFNNGNDPTNSVMEECQLPLIDLKGLRSSNEKERVA 67
           M+ S+PPL + Y  LL +ST   +P       +  +MEECQLPLIDL+GL S +E  R A
Sbjct: 1   MIHSDPPLLNHYGALLCRSTQCDEP------QSRLIMEECQLPLIDLRGLSSRDEMVRKA 60

Query: 68  CRREIFEASEEWGFFQVINHGICTELLSRMNKEQMKLFGVAFEKKFTSGILDNSYRWGTP 127
           C   I+ AS EWGFFQV+NHGI   LL +M  EQ+KLF   FE+K +  +L+NSY WGTP
Sbjct: 61  CAAAIYRASSEWGFFQVVNHGISPRLLEKMRSEQVKLFQTPFERKASCRLLNNSYTWGTP 120

Query: 128 TATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVESLG 187
           TAT P QFSWSEAFH+PLTKVS+ ACYGEF  LREVM E A+AMSKLA+ LAGVL E+LG
Sbjct: 121 TATCPKQFSWSEAFHVPLTKVSDEACYGEFTSLREVMTEFAAAMSKLARLLAGVLTENLG 180

Query: 188 HRKELLDNICDESTCFLRLNHYPICPFSGEISGLVPHTDSDFLTILHQDCGGGLQLMKGS 247
           H KE++DNICDESTCFLRLNHYP CP S EISGLVPHTDSDFLTIL QD  GGLQL K S
Sbjct: 181 HGKEVIDNICDESTCFLRLNHYPACPISPEISGLVPHTDSDFLTILCQDQVGGLQLTKDS 240

Query: 248 QWVAVKPNPEALVVNIGDLLQ 268
           +WV VKPN +AL+VNIGDL Q
Sbjct: 241 KWVTVKPNQDALIVNIGDLFQ 255

[Close]

BLAST of Cla007482 vs. NCBI nr
Match: gi|659109414|ref|XP_008454707.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X2 [Cucumis melo])

HSP 1 Score: 506.1 bits (1302), Expect = 3.8e-140
Identity = 243/271 (89.67%), Postives = 259/271 (95.57%), Query Frame = 1


Query: 7   TMMDSNPPLFHQYEFLLQSTAHLQPFNNGNDPTNSVMEECQLPLIDLKGLRSSNEKERVA 66
           TMMDSNPPLF QYEFLLQSTAHLQPFNNGN PTNSVMEECQLPLIDLKGL+SSNEKERVA
Sbjct: 8   TMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSNEKERVA 67

Query: 67  CRREIFEASEEWGFFQVINHGICTELLSRMNKEQMKLFGVAFEKKFTSGILDNSYRWGTP 126
           CRREIFEASEEWGFFQVINHGI TELL+RMNKEQ+KLFGV FEKK TSGILDNSYRWGTP
Sbjct: 68  CRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKITSGILDNSYRWGTP 127

Query: 127 TATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVESLG 186
           TATHPNQ SWSEAFHIPLTKVSEAACYG+FIYLREVMEE+ASAMSK+A+ LAGVLVES+G
Sbjct: 128 TATHPNQLSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLVESMG 187

Query: 187 HRKELLDNICDESTCFLRLNHYPICPFSGEISGLVPHTDSDFLTILHQDCGGGLQLMKGS 246
            RKELL++ICDESTCFLRLNHYPICPFSGE+SGLVPHTDSDFLTILHQD GGGLQ+MKGS
Sbjct: 188 QRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGS 247

Query: 247 QWVAVKPNPEALVVNIGDLLQVHTPNSFALV 278
           QW+AVKPNP+ALVVNIGDLLQV TP SF ++
Sbjct: 248 QWLAVKPNPQALVVNIGDLLQVQTPISFHII 278

[Close]

BLAST of Cla007482 vs. NCBI nr
Match: gi|659109410|ref|XP_008454705.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X1 [Cucumis melo])

HSP 1 Score: 506.1 bits (1302), Expect = 3.8e-140
Identity = 243/271 (89.67%), Postives = 259/271 (95.57%), Query Frame = 1


Query: 7   TMMDSNPPLFHQYEFLLQSTAHLQPFNNGNDPTNSVMEECQLPLIDLKGLRSSNEKERVA 66
           TMMDSNPPLF QYEFLLQSTAHLQPFNNGN PTNSVMEECQLPLIDLKGL+SSNEKERVA
Sbjct: 49  TMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSNEKERVA 108

Query: 67  CRREIFEASEEWGFFQVINHGICTELLSRMNKEQMKLFGVAFEKKFTSGILDNSYRWGTP 126
           CRREIFEASEEWGFFQVINHGI TELL+RMNKEQ+KLFGV FEKK TSGILDNSYRWGTP
Sbjct: 109 CRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKITSGILDNSYRWGTP 168

Query: 127 TATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVESLG 186
           TATHPNQ SWSEAFHIPLTKVSEAACYG+FIYLREVMEE+ASAMSK+A+ LAGVLVES+G
Sbjct: 169 TATHPNQLSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLVESMG 228

Query: 187 HRKELLDNICDESTCFLRLNHYPICPFSGEISGLVPHTDSDFLTILHQDCGGGLQLMKGS 246
            RKELL++ICDESTCFLRLNHYPICPFSGE+SGLVPHTDSDFLTILHQD GGGLQ+MKGS
Sbjct: 229 QRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGS 288

Query: 247 QWVAVKPNPEALVVNIGDLLQVHTPNSFALV 278
           QW+AVKPNP+ALVVNIGDLLQV TP SF ++
Sbjct: 289 QWLAVKPNPQALVVNIGDLLQVQTPISFHII 319

[Close]

BLAST of Cla007482 vs. NCBI nr
Match: gi|778698231|ref|XP_004142323.2| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus])

HSP 1 Score: 503.4 bits (1295), Expect = 2.5e-139
Identity = 243/275 (88.36%), Postives = 263/275 (95.64%), Query Frame = 1


Query: 1   MFKEDNTMM-DSNPPLFHQYEFLLQSTAHLQPFNNGNDPTNSVMEECQLPLIDLKGLRSS 60
           MFKE+ TMM DSNPPLF QYEFLLQSTAHLQPFNNGN PTNSVMEECQLPLIDLKGL+SS
Sbjct: 1   MFKENTTMMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSS 60

Query: 61  NEKERVACRREIFEASEEWGFFQVINHGICTELLSRMNKEQMKLFGVAFEKKFTSGILDN 120
           +EKERVACRREIFEASEEWGFFQVINHGI TELL+RMNKEQ+KLFGV FEKKFTSGILDN
Sbjct: 61  DEKERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDN 120

Query: 121 SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAG 180
           SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYG+FIYLREVMEE+ASAMSK+A+ LAG
Sbjct: 121 SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAG 180

Query: 181 VLVESLGHRKELLDNICDESTCFLRLNHYPICPFSGEISGLVPHTDSDFLTILHQDCGGG 240
           VLVES+G RKELL++ICDESTCFLRLNHYPICPFSGE+SGLVPHTDSDFLTILHQD GGG
Sbjct: 181 VLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGG 240

Query: 241 LQLMKGSQWVAVKPNPEALVVNIGDLLQVHTPNSF 275
           LQ+MKGSQW+AVKPNP+ALVVNIGDLLQ  + +++
Sbjct: 241 LQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTY 275

[Close]

BLAST of Cla007482 vs. NCBI nr
Match: gi|297738275|emb|CBI27476.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 346.3 bits (887), Expect = 5.0e-92
Identity = 176/273 (64.47%), Postives = 204/273 (74.73%), Query Frame = 1


Query: 7   TMMDSNPPLFHQYEFLLQ--STAHLQPFNNGNDPTNSVMEECQLPLIDLKGLRSSNEKER 66
           TM +SNPPL   Y  L Q   T  +Q  + GN+   + MEECQLPLIDL+GL S +E+ER
Sbjct: 50  TMTNSNPPLLQHYGVLCQHGETPAVQQRDCGNN--GAAMEECQLPLIDLEGLWSESEEER 109

Query: 67  VACRREIFEASEEWGFFQVINHGICTELLSRMNKEQMKLFGVAFEKKFTSGILDNSYRWG 126
           +AC   I  AS +WGFFQV+NHGI  ELLS M +EQ+KLF   FE+K    +LDNSYRWG
Sbjct: 110 LACASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWG 169

Query: 127 TPTATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVES 186
           TPTAT P + SWSEAFHIPLTKVSE ACYGEF  LREVM+E A AMS LA+ LAGVL ES
Sbjct: 170 TPTATCPKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAES 229

Query: 187 LGHRKELLDNICDESTCFLRLNHYPICPFSGEISGLVPHTDSDFLTILHQDCGGGLQLMK 246
           LGH+K + D ICDE+TCFLRLN YP CP S E+ GLVPHTDSDFLTIL+QD  GGLQLMK
Sbjct: 230 LGHQKGVFDEICDENTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMK 289

Query: 247 GSQWVAVKPNPEALVVNIGDLLQVHTPNSFALV 278
            S+WVAVKPN + L+VNIGDL Q  + N +  V
Sbjct: 290 DSKWVAVKPNKDTLIVNIGDLFQAWSNNEYKSV 320

[Close]

BLAST of Cla007482 vs. NCBI nr
Match: gi|703159524|ref|XP_010112271.1| (Gibberellin 2-beta-dioxygenase 8 [Morus notabilis])

HSP 1 Score: 344.7 bits (883), Expect = 1.5e-91
Identity = 171/270 (63.33%), Postives = 200/270 (74.07%), Query Frame = 1


Query: 8   MMDSNPPLFHQYEFLLQSTAHLQPFNNGNDPTNSVMEECQLPLIDLKGLRSSNEKERVAC 67
           ++DSNPPL   Y+ LL  ++ LQ             EECQLP+IDL GL+S    ERV C
Sbjct: 2   IIDSNPPLLDHYKALLDHSSTLQTAECDGHFDGVAAEECQLPMIDLNGLKSDEALERVEC 61

Query: 68  RREIFEASEEWGFFQVINHGICTELLSRMNKEQMKLFGVAFEKKFTSGILDNSYRWGTPT 127
            + I +A+ EWGFFQV+NHGI  EL+ +M KEQ KLF   FE K TS +L+NSYRWGTPT
Sbjct: 62  AKAISKAASEWGFFQVVNHGISLELVKKMRKEQTKLFEAPFEDKATSRLLNNSYRWGTPT 121

Query: 128 ATHPNQFSWSEAFHIPLTKVSEAACYGEFIYLREVMEELASAMSKLAKSLAGVLVESLGH 187
           AT P+QFSWSEAFHIPLTKVSE  CYG+FI LRE  EE A+AMSKLA+SLAGVL E+LGH
Sbjct: 122 ATRPSQFSWSEAFHIPLTKVSEEDCYGDFISLREPTEEFAAAMSKLARSLAGVLAENLGH 181

Query: 188 RKELLDNICDESTCFLRLNHYPICPFSGEISGLVPHTDSDFLTILHQDCGGGLQLMKGSQ 247
           R+++ + ICDESTCFLRLN YP C  S EI GLVPHTDS FLTIL QD  GGLQLMK  +
Sbjct: 182 RRQVFEEICDESTCFLRLNRYPACAVSSEIFGLVPHTDSGFLTILRQDHVGGLQLMKDYK 241

Query: 248 WVAVKPNPEALVVNIGDLLQVHTPNSFALV 278
           WV+VKPNPEAL+VNIGDL Q  + + F  V
Sbjct: 242 WVSVKPNPEALIVNIGDLFQAWSNDVFKSV 271

The following BLAST results are available for this feature:

BLAST of Cla007482 vs. Swiss-Prot


Match Name	E-value	Identity	Description
G2OX8_ARATH	1.3e-60	51.32	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2	[more]
G2OX7_ARATH	1.0e-44	39.31	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1	[more]
ACCO2_DORSP	4.1e-25	34.01	1-aminocyclopropane-1-carboxylate oxidase 2 OS=Doritaenopsis sp. GN=ACO2 PE=2 SV...	[more]
ACCO_PRUMU	4.1e-25	33.05	1-aminocyclopropane-1-carboxylate oxidase OS=Prunus mume GN=ACO1 PE=2 SV=1	[more]
ACCO_DIACA	6.9e-25	33.47	Probable 1-aminocyclopropane-1-carboxylate oxidase OS=Dianthus caryophyllus GN=A...	[more]

BLAST of Cla007482 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KIW0_CUCSA	1.7e-139	88.36	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G052540 PE=3 SV=1	[more]
F6HG07_VITVI	3.5e-92	64.47	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g01650 PE=3 SV=...	[more]
W9SYI1_9ROSA	1.0e-91	63.33	Gibberellin 2-beta-dioxygenase 8 OS=Morus notabilis GN=L484_014953 PE=3 SV=1	[more]
A5BF50_VITVI	3.9e-91	64.34	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_025459 PE=3 SV=1	[more]
A0A061E9S8_THECC	1.1e-90	65.90	Gibberellin 2-oxidase 8 OS=Theobroma cacao GN=TCM_011301 PE=3 SV=1	[more]

BLAST of Cla007482 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659109414\|ref\|XP_008454707.1\|	3.8e-140	89.67	PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X2 [Cucumis melo]	[more]
gi\|659109410\|ref\|XP_008454705.1\|	3.8e-140	89.67	PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X1 [Cucumis melo]	[more]
gi\|778698231\|ref\|XP_004142323.2\|	2.5e-139	88.36	PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus]	[more]
gi\|297738275\|emb\|CBI27476.3\|	5.0e-92	64.47	unnamed protein product [Vitis vinifera]	[more]
gi\|703159524\|ref\|XP_010112271.1\|	1.5e-91	63.33	Gibberellin 2-beta-dioxygenase 8 [Morus notabilis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR005123	Oxoglu/Fe-dep_dioxygenase
IPR026992	DIOX_N
IPR027443	IPNS-like

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0051213	dioxygenase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla007482	Cla007482.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 202..272 score: 1.2
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROFILE	PS51471	FE2OG_OXY	coord: 197..279 score: 8
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 48..142 score: 3.4
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 38..272 score: 4.3
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 1..267 score: 1.0E
None	No IPR available	PANTHER	PTHR10209:SF177	SUBFAMILY NOT NAMED	coord: 1..267 score: 1.0E
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 37..272 score: 6.73

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cla007482	Csa5G052540	Cucumber (Chinese Long) v2	cuwmB389
Cla007482	ClCG07G007000	Watermelon (Charleston Gray)	wcgwmB376
Cla007482	CSPI05G01270	Wild cucumber (PI 183967)	cpiwmB397
Cla007482	Lsi04G011840	Bottle gourd (USVL1VR-Ls)	lsiwmB308
Cla007482	CsGy5G011470	Cucumber (Gy14) v2	cgybwmB361
Cla007482	CsaV3_5G015180	Cucumber (Chinese Long) v3	cucwmB412

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla007482	Watermelon (97103) v2	wmwmbB178