Cucsa.372030 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.372030
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionGibberellin 2-oxidase
Locationscaffold03703 : 64862 .. 66526 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACTCTAATCCACCACTATTCGATCAATACGAGTTCCTTTTGCAATCCACAGCTCATTTACAACCATTTAACAATGGAAATCTACCAACTAACTCTGTCATGGAGGAATGTCAGCTTCCTCTTATTGATCTTAAAGGTTTGAAAAGTAGCGATGAAAAGGAGAGGGTAGCTTGTAGAAGAGAGATTTTTGAAGCATCAGAAGAATGGGGATTCTTCCAAGTGATAAACCATGGGATACACACAGAGCTACTGAACAGAATGAACAAAGAACAAATAAAGTTGTTTGGGGTACCCTTTGAGAAAAAATTTACAAGTGGGATTTTGGATAATTCTTACAGATGGGGAACACCCACTGCAACCCACCCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGCCTGCTATGGCGACTTCATTTACTTAAGGTATCAATTCTATCTATCAACATTCATCCAGCTATATACTTCTCTTAAGCATTATCATTATTACATTGTTAGTTTCAATACTTAAGAACACTGTGCCTATATCAAACTATATGGCCTTTTAGACTTGAAAAAGTTTTAGATTAATCAGACTATGCTTTTACTTGTTAATAATATCACATTTACAGAAGTTACCTAATCATCATATCCCATTCATATTTTAGTTTAAAGTTTTAGGAACATGATTGAATGATTTTATAAAGTTCGATTTAAAATCTAATTGTTCAAAGACATCTAGCGAACTTGAAGAGTCTAGCTCTTGTAGACATTTTCACAAACAGTTAGAGCGCAAAAGGTAACGACACTTCATTCTTCCGCAGATATACTAAATTAGCAAACAATAAGAAAAGCCCATTAATGAATTGACTGACCTTACATCATGGAACGGCAGGGAAGTGATGGAGGAAGTGGCAAGCGCGATGTCGAAGGTAGCAAGAAAATTGGCAGGAGTTTTAGTGGAGAGTATGGGGCAGAGGAAGGAATTATTAGAGGATATTTGCGACGAAAGCACATGCTTCCTCCGGTTGAATCACTACCCAATTTGTCCATTTTCCGGCGAAGTTTCAGGGCTGGTGCCACACACGGACAGCGATTTTCTTACAATACTTCATCAAGATTCCGGCGGTGGTCTTCAGGTGATGAAAGGATCGCAATGGTTAGCTGTGAAACCCAATCCACAAGCCCTTGTTGTCAACATTGGAGACCTTCTTCAGGTAAACACTCCAACTCCAATTTCTTTTCATCCAATCATTTACCATTCACTCTTTTTTCAGTTTCCATATTACCTAATCTATTGACATTAATATTGAGAAATTCTTTATGAATATTGTATAAACAAATTTACCTTAAAAAAAAATTAAGATAGATTTTAGTGATCAAAAGTCTATGACTTGAACACTTGAATGTGGTGGACCAAATTTTTAACATATTCTCTAGAACAACACAAAGTATTTTATGTTAAATATGTGTCAAGTGAACATACTTCAAACATACGAATTTATGAACAGCTAAGAAATCTGTATTTTGAATTTCATCTTTTCAATATATATCTTCATTTCAATTATGCAATCTAAGATAGTATAACTATTTATTATTACGATAGTCATTATTACCAATACCCCAAAGTGAACACTTTGGAGAAAAGAAAGAATGA

mRNA sequence

ATGGACTCTAATCCACCACTATTCGATCAATACGAGTTCCTTTTGCAATCCACAGCTCATTTACAACCATTTAACAATGGAAATCTACCAACTAACTCTGTCATGGAGGAATGTCAGCTTCCTCTTATTGATCTTAAAGGTTTGAAAAGTAGCGATGAAAAGGAGAGGGTAGCTTGTAGAAGAGAGATTTTTGAAGCATCAGAAGAATGGGGATTCTTCCAAGTGATAAACCATGGGATACACACAGAGCTACTGAACAGAATGAACAAAGAACAAATAAAGTTGTTTGGGGTACCCTTTGAGAAAAAATTTACAAGTGGGATTTTGGATAATTCTTACAGATGGGGAACACCCACTGCAACCCACCCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGCCCATTAATGAATTGACTGACCTTACATCATGGAACGGCAGGGAAGTGATGGAGGAAGTGGCAAGCGCGATGTCGAAGGTAGCAAGAAAATTGGCAGGAGTTTTAGTGGAGAGTATGGGGCAGAGGAAGGAATTATTAGAGGATATTTGCGACGAAAGCACATGCTTCCTCCGGTTGAATCACTACCCAATTTGTCCATTTTCCGGCGAAGTTTCAGGGCTGGTGCCACACACGGACAGCGATTTTCTTACAATACTTCATCAAGATTCCGGCGGTGGTCTTCAGGTGATGAAAGGATCGCAATGGTTAGCTGTGAAACCCAATCCACAAGCCCTTGTTGTCAACATTGGAGACCTTCTTCAGTCATTATTACCAATACCCCAAAGTGAACACTTTGGAGAAAAGAAAGAATGA

Coding sequence (CDS)

ATGGACTCTAATCCACCACTATTCGATCAATACGAGTTCCTTTTGCAATCCACAGCTCATTTACAACCATTTAACAATGGAAATCTACCAACTAACTCTGTCATGGAGGAATGTCAGCTTCCTCTTATTGATCTTAAAGGTTTGAAAAGTAGCGATGAAAAGGAGAGGGTAGCTTGTAGAAGAGAGATTTTTGAAGCATCAGAAGAATGGGGATTCTTCCAAGTGATAAACCATGGGATACACACAGAGCTACTGAACAGAATGAACAAAGAACAAATAAAGTTGTTTGGGGTACCCTTTGAGAAAAAATTTACAAGTGGGATTTTGGATAATTCTTACAGATGGGGAACACCCACTGCAACCCACCCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTCCCCTCACAAAGCCCATTAATGAATTGACTGACCTTACATCATGGAACGGCAGGGAAGTGATGGAGGAAGTGGCAAGCGCGATGTCGAAGGTAGCAAGAAAATTGGCAGGAGTTTTAGTGGAGAGTATGGGGCAGAGGAAGGAATTATTAGAGGATATTTGCGACGAAAGCACATGCTTCCTCCGGTTGAATCACTACCCAATTTGTCCATTTTCCGGCGAAGTTTCAGGGCTGGTGCCACACACGGACAGCGATTTTCTTACAATACTTCATCAAGATTCCGGCGGTGGTCTTCAGGTGATGAAAGGATCGCAATGGTTAGCTGTGAAACCCAATCCACAAGCCCTTGTTGTCAACATTGGAGACCTTCTTCAGTCATTATTACCAATACCCCAAAGTGAACACTTTGGAGAAAAGAAAGAATGA

Protein sequence

MDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSDEKERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKPINELTDLTSWNGREVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQSLLPIPQSEHFGEKKE*
Homology
[Close]
BLAST of Cucsa.372030 vs. Swiss-Prot
Match: G2OX8_ARATH (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 239.6 bits (610), Expect = 4.1e-62
Identity = 125/262 (47.71%), Postives = 170/262 (64.89%), Query Frame = 1 

Query: 4   NPPLFDQYEFLLQSTAHLQPFNNGNLP----TNSVMEECQLPLIDLKGLKSSDEKERVAC 63
           +PP  + Y  LL +    +  +   +P      +V+EE +LP+ID+  L    E+ER  C
Sbjct: 2   DPPFNEIYNNLLYNQITKKDNDVSEIPFSFSVTAVVEEVELPVIDVSRLIDGAEEEREKC 61

Query: 64  RREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILD-NSYRWGTP 123
           +  I  AS EWGFFQVINHGI  ++L +M +EQI++F  PF+KK  S      SYRWGTP
Sbjct: 62  KEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEKFSAGSYRWGTP 121

Query: 124 TATHPNQFSWSEAFHIPLTKPINELTDLTSWNGREVMEEVASAMSKVARKLAGVLVESMG 183
           +AT   Q SWSEAFH+P+T  I++  D T+ +    ME+ AS    +A  LA VL E  G
Sbjct: 122 SATSIRQLSWSEAFHVPMTD-ISDNKDFTTLSS--TMEKFASESEALAYMLAEVLAEKSG 181

Query: 184 QRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGS 243
           Q     ++ C  +TC+LR+N YP CP   EV GL+PHTDSDFLTIL+QD  GGLQ++K +
Sbjct: 182 QNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLIKDN 241

Query: 244 QWLAVKPNPQALVVNIGDLLQS 261
           +W+AVKPNP+AL++NIGDL Q+
Sbjct: 242 RWIAVKPNPKALIINIGDLFQA 260

[Close]
BLAST of Cucsa.372030 vs. Swiss-Prot
Match: G2OX7_ARATH (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.6e-45
Identity = 101/240 (42.08%), Postives = 147/240 (61.25%), Query Frame = 1 

Query: 25  NNGNLPTNSVMEECQLPLIDLKGLKSSDEKERVACRREIFEASEEWGFFQVINHGIHTEL 84
           N   + T+ +    +LP+IDL  L S +E +R  C +++  A++EWGFFQ++NHGI  ++
Sbjct: 29  NTSTIQTSGI----KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDV 88

Query: 85  LNRMNKEQIKLFGVPFEKKFTSGILD---NSYRWGTPTATHPNQFSWSEAFHIPLTKPIN 144
              M  E+ KLF  PF  K      D   NSYRWG P+AT P Q+S SEAFHI L++ ++
Sbjct: 89  FEMMLLEEKKLFDQPFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSE-VS 148

Query: 145 ELTDLTSWNGREVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYP 204
            ++D  + N R ++E     +++VA+ +  +L + +    E  E+I +    FLRLN Y 
Sbjct: 149 RISDDRN-NLRTIVETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYH 208

Query: 205 ICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQSL 262
              F  EV GLVPHTD+ FLTIL QD  GGL++    QW++VKP  +AL VNIGD+ Q+L
Sbjct: 209 PSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQAL 262

[Close]
BLAST of Cucsa.372030 vs. Swiss-Prot
Match: GAO1B_WHEAT (Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.1e-25
Identity = 82/245 (33.47%), Postives = 118/245 (48.16%), Query Frame = 1 

Query: 27  GNLPTNSVMEECQLPLIDLKGLKSSDEKERVACRREIFEASEEWGFFQVINHGIHTELLN 86
           G  PT    EE  +PLID+ G+ S D +      R + EA E  GFFQV+NHGI  ELL 
Sbjct: 26  GESPTPDAAEELHVPLIDIGGMLSGDPRATAEVTRLVGEACERHGFFQVVNHGIDAELLA 85

Query: 87  RMNKEQIKLFGVPF-EKKFTSGILDNSYRWGTP-TATHPNQFSWSE--AFHIPLTKPINE 146
             ++     F +P  EK+        S  + +  T    ++  W E  +F    + P   
Sbjct: 86  DAHRCVDAFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKETLSFRSCPSDPALV 145

Query: 147 LTDLTSWNGR------EVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLR 206
           +  + +  G       EV     S MS+++ ++  VL ES+G  +       + +   +R
Sbjct: 146 VDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAHYRRFFEGNDSIMR 205

Query: 207 LNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGD 262
           LN+YP C    E  G  PH D   LTILHQD+ GGLQV    +W +++P   A VVNIGD
Sbjct: 206 LNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPRADAFVVNIGD 265

[Close]
BLAST of Cucsa.372030 vs. Swiss-Prot
Match: GAO1D_WHEAT (Gibberellin 20 oxidase 1-D OS=Triticum aestivum GN=GA20ox1D PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.4e-25
Identity = 82/245 (33.47%), Postives = 117/245 (47.76%), Query Frame = 1 

Query: 27  GNLPTNSVMEECQLPLIDLKGLKSSDEKERVACRREIFEASEEWGFFQVINHGIHTELLN 86
           G  PT    EE  +PLID+ G+ S D        R + EA E  GFFQV+NHGI  ELL 
Sbjct: 26  GESPTPDAAEELHVPLIDIGGMLSGDPAAAAEVTRLVGEACERHGFFQVVNHGIDAELLA 85

Query: 87  RMNKEQIKLFGVPF-EKKFTSGILDNSYRWGTP-TATHPNQFSWSE--AFHIPLTKPINE 146
             ++     F +P  EK+        S  + +  T    ++  W E  +F    + P   
Sbjct: 86  DAHRCVDNFFTMPLPEKQRALRHPGESCGYASSFTGRFASKLPWKETLSFRSCPSDPALV 145

Query: 147 LTDLTSWNGR------EVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLR 206
           +  + +  G       EV     S MS+++ ++  VL ES+G  +       + +   +R
Sbjct: 146 VDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAHYRRFFEGNDSIMR 205

Query: 207 LNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGD 262
           LN+YP C    E  G  PH D   LTILHQD+ GGLQV    +W +++P   A VVNIGD
Sbjct: 206 LNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPRADAFVVNIGD 265

[Close]
BLAST of Cucsa.372030 vs. Swiss-Prot
Match: GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 115.9 bits (289), Expect = 6.9e-25
Identity = 77/239 (32.22%), Postives = 120/239 (50.21%), Query Frame = 1 

Query: 37  ECQLPLIDLKGLKSSDEKERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLF 96
           E  +PLIDL+ L S D    +   R I EA ++ GFF V+NHGI  EL++  ++   + F
Sbjct: 58  ELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLVVNHGISEELISDAHEYTSRFF 117

Query: 97  GVPFEKKFTSGILDNSYRWGTPTATHPNQFS----WSEAFHIPLTKPINELTDLTSW--- 156
            +P  +K    +L  S       ++   +FS    W E         ++    +  +   
Sbjct: 118 DMPLSEK--QRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRFCDDMSRSKSVQDYFCD 177

Query: 157 ---NGRE----VMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPI 216
              +G +    V +E   AMS ++ K+  +L  S+G +++   +  +E+   +RLN+YP 
Sbjct: 178 ALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFREFFEENDSIMRLNYYPP 237

Query: 217 CPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQSL 262
           C       G  PH D   LTILHQD   GLQV   +QW +++PNP+A VVNIGD   +L
Sbjct: 238 CIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNPKAFVVNIGDTFMAL 293

[Close]
BLAST of Cucsa.372030 vs. TrEMBL
Match: A0A0A0KIW0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G052540 PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 2.1e-137
Identity = 246/260 (94.62%), Postives = 247/260 (95.00%), Query Frame = 1 

Query: 1   MDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSDEKERVACR 60
           MDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSDEKERVACR
Sbjct: 10  MDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSDEKERVACR 69

Query: 61  REIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSYRWGTPTA 120
           REIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSYRWGTPTA
Sbjct: 70  REIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSYRWGTPTA 129

Query: 121 THPNQFSWSEAFHIPLTKPINELTDLTSWNGREVMEEVASAMSKVARKLAGVLVESMGQR 180
           THPNQFSWSEAFHIPLTK             REVMEEVASAMSKVARKLAGVLVESMGQR
Sbjct: 130 THPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLVESMGQR 189

Query: 181 KELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQW 240
           KELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQW
Sbjct: 190 KELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQW 249

Query: 241 LAVKPNPQALVVNIGDLLQS 261
           LAVKPNPQALVVNIGDLLQ+
Sbjct: 250 LAVKPNPQALVVNIGDLLQA 269

[Close]
BLAST of Cucsa.372030 vs. TrEMBL
Match: W9SYI1_9ROSA (Gibberellin 2-beta-dioxygenase 8 OS=Morus notabilis GN=L484_014953 PE=3 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 1.0e-83
Identity = 158/260 (60.77%), Postives = 190/260 (73.08%), Query Frame = 1 

Query: 1   MDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSDEKERVACR 60
           +DSNPPL D Y+ LL  ++ LQ             EECQLP+IDL GLKS +  ERV C 
Sbjct: 3   IDSNPPLLDHYKALLDHSSTLQTAECDGHFDGVAAEECQLPMIDLNGLKSDEALERVECA 62

Query: 61  REIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSYRWGTPTA 120
           + I +A+ EWGFFQV+NHGI  EL+ +M KEQ KLF  PFE K TS +L+NSYRWGTPTA
Sbjct: 63  KAISKAASEWGFFQVVNHGISLELVKKMRKEQTKLFEAPFEDKATSRLLNNSYRWGTPTA 122

Query: 121 THPNQFSWSEAFHIPLTKPINELTDLTSWNGREVMEEVASAMSKVARKLAGVLVESMGQR 180
           T P+QFSWSEAFHIPLTK   E       + RE  EE A+AMSK+AR LAGVL E++G R
Sbjct: 123 TRPSQFSWSEAFHIPLTKVSEEDCYGDFISLREPTEEFAAAMSKLARSLAGVLAENLGHR 182

Query: 181 KELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQW 240
           +++ E+ICDESTCFLRLN YP C  S E+ GLVPHTDS FLTIL QD  GGLQ+MK  +W
Sbjct: 183 RQVFEEICDESTCFLRLNRYPACAVSSEIFGLVPHTDSGFLTILRQDHVGGLQLMKDYKW 242

Query: 241 LAVKPNPQALVVNIGDLLQS 261
           ++VKPNP+AL+VNIGDL Q+
Sbjct: 243 VSVKPNPEALIVNIGDLFQA 262

[Close]
BLAST of Cucsa.372030 vs. TrEMBL
Match: M5XWN4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008125mg PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 1.9e-82
Identity = 162/268 (60.45%), Postives = 192/268 (71.64%), Query Frame = 1 

Query: 1   MDSNPPLFDQYEFLLQSTAHL-----QPFNNGNLPTNSVMEECQLPLIDLKGLKSSDEKE 60
           ++SNPPL   Y  L+++ ++L     Q    G +P     EECQLPLIDL GLK  D +E
Sbjct: 2   IESNPPLLHHYGTLVRNPSNLVQVDQQRHIGGLVPA----EECQLPLIDLHGLKIPDTRE 61

Query: 61  RVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSYRW 120
            + C R I  AS EWGFFQV+NHGI  ELL  M +EQ+KLF  PFE+K T G+L+NSYRW
Sbjct: 62  SLECARAICRASSEWGFFQVLNHGISPELLQSMRREQLKLFAAPFERKATCGLLNNSYRW 121

Query: 121 GTPTATHPNQFSWSEAFHIPLTKPINELT---DLTSWNGREVMEEVASAMSKVARKLAGV 180
           GTPTAT P Q+SWSEAFHIP+TK   E +   D  S  G  VMEE A+AMS +A  LAG+
Sbjct: 122 GTPTATRPTQYSWSEAFHIPVTKISEEASYGEDFGSLRG--VMEEFAAAMSNLANLLAGI 181

Query: 181 LVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGL 240
           L  ++G RKE LEDICD STCFLRLN YP CP S E+ GLVPHTDSDFLTIL QD  GGL
Sbjct: 182 LANNLGHRKESLEDICDSSTCFLRLNRYPACPISPEMFGLVPHTDSDFLTILCQDQVGGL 241

Query: 241 QVMKGSQWLAVKPNPQALVVNIGDLLQS 261
           Q+MK S+W+AVKPNP AL+VNIGDL Q+
Sbjct: 242 QLMKDSKWVAVKPNPDALIVNIGDLFQA 263

[Close]
BLAST of Cucsa.372030 vs. TrEMBL
Match: F6HG07_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g01650 PE=3 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 7.3e-82
Identity = 159/261 (60.92%), Postives = 189/261 (72.41%), Query Frame = 1 

Query: 2   DSNPPLFDQYEFLLQ--STAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSDEKERVAC 61
           +SNPPL   Y  L Q   T  +Q  + GN    + MEECQLPLIDL+GL S  E+ER+AC
Sbjct: 26  NSNPPLLQHYGVLCQHGETPAVQQRDCGN--NGAAMEECQLPLIDLEGLWSESEEERLAC 85

Query: 62  RREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSYRWGTPT 121
              I  AS +WGFFQV+NHGI  ELL+ M +EQ+KLF  PFE+K    +LDNSYRWGTPT
Sbjct: 86  ASAIGRASSKWGFFQVVNHGIRPELLSEMRREQVKLFETPFERKAACQLLDNSYRWGTPT 145

Query: 122 ATHPNQFSWSEAFHIPLTKPINELTDLTSWNGREVMEEVASAMSKVARKLAGVLVESMGQ 181
           AT P + SWSEAFHIPLTK   E       + REVM+E A AMS +AR LAGVL ES+G 
Sbjct: 146 ATCPKELSWSEAFHIPLTKVSEEACYGEFCSLREVMQEFAKAMSNLARLLAGVLAESLGH 205

Query: 182 RKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQ 241
           +K + ++ICDE+TCFLRLN YP CP S EV GLVPHTDSDFLTIL+QD  GGLQ+MK S+
Sbjct: 206 QKGVFDEICDENTCFLRLNRYPPCPVSPEVFGLVPHTDSDFLTILYQDEVGGLQLMKDSK 265

Query: 242 WLAVKPNPQALVVNIGDLLQS 261
           W+AVKPN   L+VNIGDL Q+
Sbjct: 266 WVAVKPNKDTLIVNIGDLFQA 284

[Close]
BLAST of Cucsa.372030 vs. TrEMBL
Match: A0A061E9S8_THECC (Gibberellin 2-oxidase 8 OS=Theobroma cacao GN=TCM_011301 PE=3 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 7.3e-82
Identity = 158/259 (61.00%), Postives = 190/259 (73.36%), Query Frame = 1 

Query: 3   SNPPLFDQYEFLL-QSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSDEKERVACRR 62
           S+PPL + Y  LL +ST   +P       +  +MEECQLPLIDL+GL S DE  R AC  
Sbjct: 4   SDPPLLNHYGALLCRSTQCDEP------QSRLIMEECQLPLIDLRGLSSRDEMVRKACAA 63

Query: 63  EIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSYRWGTPTAT 122
            I+ AS EWGFFQV+NHGI   LL +M  EQ+KLF  PFE+K +  +L+NSY WGTPTAT
Sbjct: 64  AIYRASSEWGFFQVVNHGISPRLLEKMRSEQVKLFQTPFERKASCRLLNNSYTWGTPTAT 123

Query: 123 HPNQFSWSEAFHIPLTKPINELTDLTSWNGREVMEEVASAMSKVARKLAGVLVESMGQRK 182
            P QFSWSEAFH+PLTK  +E       + REVM E A+AMSK+AR LAGVL E++G  K
Sbjct: 124 CPKQFSWSEAFHVPLTKVSDEACYGEFTSLREVMTEFAAAMSKLARLLAGVLTENLGHGK 183

Query: 183 ELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQWL 242
           E++++ICDESTCFLRLNHYP CP S E+SGLVPHTDSDFLTIL QD  GGLQ+ K S+W+
Sbjct: 184 EVIDNICDESTCFLRLNHYPACPISPEISGLVPHTDSDFLTILCQDQVGGLQLTKDSKWV 243

Query: 243 AVKPNPQALVVNIGDLLQS 261
            VKPN  AL+VNIGDL Q+
Sbjct: 244 TVKPNQDALIVNIGDLFQA 256

[Close]
BLAST of Cucsa.372030 vs. TAIR10
Match: AT4G21200.1 (AT4G21200.1 gibberellin 2-oxidase 8)

HSP 1 Score: 239.6 bits (610), Expect = 2.3e-63
Identity = 125/262 (47.71%), Postives = 170/262 (64.89%), Query Frame = 1 

Query: 4   NPPLFDQYEFLLQSTAHLQPFNNGNLP----TNSVMEECQLPLIDLKGLKSSDEKERVAC 63
           +PP  + Y  LL +    +  +   +P      +V+EE +LP+ID+  L    E+ER  C
Sbjct: 2   DPPFNEIYNNLLYNQITKKDNDVSEIPFSFSVTAVVEEVELPVIDVSRLIDGAEEEREKC 61

Query: 64  RREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILD-NSYRWGTP 123
           +  I  AS EWGFFQVINHGI  ++L +M +EQI++F  PF+KK  S      SYRWGTP
Sbjct: 62  KEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEKFSAGSYRWGTP 121

Query: 124 TATHPNQFSWSEAFHIPLTKPINELTDLTSWNGREVMEEVASAMSKVARKLAGVLVESMG 183
           +AT   Q SWSEAFH+P+T  I++  D T+ +    ME+ AS    +A  LA VL E  G
Sbjct: 122 SATSIRQLSWSEAFHVPMTD-ISDNKDFTTLSS--TMEKFASESEALAYMLAEVLAEKSG 181

Query: 184 QRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGS 243
           Q     ++ C  +TC+LR+N YP CP   EV GL+PHTDSDFLTIL+QD  GGLQ++K +
Sbjct: 182 QNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLIKDN 241

Query: 244 QWLAVKPNPQALVVNIGDLLQS 261
           +W+AVKPNP+AL++NIGDL Q+
Sbjct: 242 RWIAVKPNPKALIINIGDLFQA 260

[Close]
BLAST of Cucsa.372030 vs. TAIR10
Match: AT1G50960.1 (AT1G50960.1 gibberellin 2-oxidase 7)

HSP 1 Score: 184.5 bits (467), Expect = 8.9e-47
Identity = 101/240 (42.08%), Postives = 147/240 (61.25%), Query Frame = 1 

Query: 25  NNGNLPTNSVMEECQLPLIDLKGLKSSDEKERVACRREIFEASEEWGFFQVINHGIHTEL 84
           N   + T+ +    +LP+IDL  L S +E +R  C +++  A++EWGFFQ++NHGI  ++
Sbjct: 29  NTSTIQTSGI----KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDV 88

Query: 85  LNRMNKEQIKLFGVPFEKKFTSGILD---NSYRWGTPTATHPNQFSWSEAFHIPLTKPIN 144
              M  E+ KLF  PF  K      D   NSYRWG P+AT P Q+S SEAFHI L++ ++
Sbjct: 89  FEMMLLEEKKLFDQPFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSE-VS 148

Query: 145 ELTDLTSWNGREVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYP 204
            ++D  + N R ++E     +++VA+ +  +L + +    E  E+I +    FLRLN Y 
Sbjct: 149 RISDDRN-NLRTIVETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYH 208

Query: 205 ICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQSL 262
              F  EV GLVPHTD+ FLTIL QD  GGL++    QW++VKP  +AL VNIGD+ Q+L
Sbjct: 209 PSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQAL 262

[Close]
BLAST of Cucsa.372030 vs. TAIR10
Match: AT3G19000.1 (AT3G19000.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 121.3 bits (303), Expect = 9.2e-28
Identity = 86/240 (35.83%), Postives = 121/240 (50.42%), Query Frame = 1 

Query: 39  QLPLIDLKGLKSSDEKERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGV 98
           ++P IDL  L+ +   ++ A  +EI EA + WGFFQVINHG+ + L +R+ K   + F +
Sbjct: 31  EIPTIDLSSLEDTHH-DKTAIAKEIAEACKRWGFFQVINHGLPSALRHRVEKTAAEFFNL 90

Query: 99  PFEKKFTSGILDNSYRWGTPTATHP-NQFSWSEAFHIPL-------TKPINELTDL---- 158
             E+K      D     G     H  N   W E F   L         P  E T+L    
Sbjct: 91  TTEEK-RKVKRDEVNPMGYHDEEHTKNVRDWKEIFDFFLQDSTIVPASPEPEDTELRKLT 150

Query: 159 TSW-----NGREVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYP 218
             W     + REV +E A  + K+A +L  ++  S+G   + L    +E T FLR NHYP
Sbjct: 151 NQWPQNPSHFREVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNEQTSFLRFNHYP 210

Query: 219 ICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGS--QWLAVKPNPQALVVNIGDLLQ 260
            CP      G+  H D   LT+L QDS GGLQV + S  QW+ VKP   AL++N+G+ +Q
Sbjct: 211 PCPNPELALGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIINMGNCIQ 268

[Close]
BLAST of Cucsa.372030 vs. TAIR10
Match: AT4G25420.1 (AT4G25420.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 115.9 bits (289), Expect = 3.9e-26
Identity = 77/239 (32.22%), Postives = 120/239 (50.21%), Query Frame = 1 

Query: 37  ECQLPLIDLKGLKSSDEKERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLF 96
           E  +PLIDL+ L S D    +   R I EA ++ GFF V+NHGI  EL++  ++   + F
Sbjct: 58  ELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLVVNHGISEELISDAHEYTSRFF 117

Query: 97  GVPFEKKFTSGILDNSYRWGTPTATHPNQFS----WSEAFHIPLTKPINELTDLTSW--- 156
            +P  +K    +L  S       ++   +FS    W E         ++    +  +   
Sbjct: 118 DMPLSEK--QRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRFCDDMSRSKSVQDYFCD 177

Query: 157 ---NGRE----VMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPI 216
              +G +    V +E   AMS ++ K+  +L  S+G +++   +  +E+   +RLN+YP 
Sbjct: 178 ALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFREFFEENDSIMRLNYYPP 237

Query: 217 CPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQSL 262
           C       G  PH D   LTILHQD   GLQV   +QW +++PNP+A VVNIGD   +L
Sbjct: 238 CIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNPKAFVVNIGDTFMAL 293

[Close]
BLAST of Cucsa.372030 vs. TAIR10
Match: AT4G25300.1 (AT4G25300.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 114.4 bits (285), Expect = 1.1e-25
Identity = 73/237 (30.80%), Postives = 124/237 (52.32%), Query Frame = 1 

Query: 39  QLPLIDLKGLKSSDEKERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGV 98
           Q+P+ID+  L SS   +    + +   A +EWGFFQ++NHG+ +  LN++  E    F +
Sbjct: 51  QIPIIDMSLLCSSTSMDSEIDKLD--SACKEWGFFQLVNHGMESSFLNKVKSEVQDFFNL 110

Query: 99  PFEKKFTSGILDNSYRWGTP----------TATHPNQFSWSEAFHIPLTKPIN----ELT 158
           P E+K           W  P            +   +  W++ F + + +P+      L 
Sbjct: 111 PMEEK--------KNLWQQPDEIEGFGQVFVVSEEQKLDWADMFFLTM-QPVRLRKPHLF 170

Query: 159 DLTSWNGREVMEEVASAMSKVARKLAGVLVESMGQRKELLEDIC-DESTCFLRLNHYPIC 218
                  R+ ++  ++ +  +A+ L G +  ++  + E ++ +  DE    +RLN+YP C
Sbjct: 171 PKLPLPFRDTLDMYSAEVKSIAKILLGKIAVALKIKPEEMDKLFDDELGQRIRLNYYPRC 230

Query: 219 PFSGEVSGLVPHTDSDFLTILHQ-DSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQ 260
           P   +V GL PH+DS  LTIL Q +   GLQ+ K ++W++VKP P ALVVN+GD+L+
Sbjct: 231 PEPDKVIGLTPHSDSTGLTILLQANEVEGLQIKKNAKWVSVKPLPNALVVNVGDILE 276

[Close]
BLAST of Cucsa.372030 vs. NCBI nr
Match: gi|778698231|ref|XP_004142323.2| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus])

HSP 1 Score: 496.5 bits (1277), Expect = 3.0e-137
Identity = 246/260 (94.62%), Postives = 247/260 (95.00%), Query Frame = 1 

Query: 1   MDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSDEKERVACR 60
           MDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSDEKERVACR
Sbjct: 10  MDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSDEKERVACR 69

Query: 61  REIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSYRWGTPTA 120
           REIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSYRWGTPTA
Sbjct: 70  REIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSYRWGTPTA 129

Query: 121 THPNQFSWSEAFHIPLTKPINELTDLTSWNGREVMEEVASAMSKVARKLAGVLVESMGQR 180
           THPNQFSWSEAFHIPLTK             REVMEEVASAMSKVARKLAGVLVESMGQR
Sbjct: 130 THPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLVESMGQR 189

Query: 181 KELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQW 240
           KELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQW
Sbjct: 190 KELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQW 249

Query: 241 LAVKPNPQALVVNIGDLLQS 261
           LAVKPNPQALVVNIGDLLQ+
Sbjct: 250 LAVKPNPQALVVNIGDLLQA 269

[Close]
BLAST of Cucsa.372030 vs. NCBI nr
Match: gi|659109414|ref|XP_008454707.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X2 [Cucumis melo])

HSP 1 Score: 491.9 bits (1265), Expect = 7.4e-136
Identity = 245/264 (92.80%), Postives = 246/264 (93.18%), Query Frame = 1 

Query: 1   MDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSDEKERVACR 60
           MDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSS+EKERVACR
Sbjct: 10  MDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSNEKERVACR 69

Query: 61  REIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSYRWGTPTA 120
           REIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKK TSGILDNSYRWGTPTA
Sbjct: 70  REIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKITSGILDNSYRWGTPTA 129

Query: 121 THPNQFSWSEAFHIPLTKPINELTDLTSWNGREVMEEVASAMSKVARKLAGVLVESMGQR 180
           THPNQ SWSEAFHIPLTK             REVMEEVASAMSKVARKLAGVLVESMGQR
Sbjct: 130 THPNQLSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLVESMGQR 189

Query: 181 KELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQW 240
           KELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQW
Sbjct: 190 KELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQW 249

Query: 241 LAVKPNPQALVVNIGDLLQSLLPI 265
           LAVKPNPQALVVNIGDLLQ   PI
Sbjct: 250 LAVKPNPQALVVNIGDLLQVQTPI 273

[Close]
BLAST of Cucsa.372030 vs. NCBI nr
Match: gi|659109410|ref|XP_008454705.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X1 [Cucumis melo])

HSP 1 Score: 491.9 bits (1265), Expect = 7.4e-136
Identity = 245/264 (92.80%), Postives = 246/264 (93.18%), Query Frame = 1 

Query: 1   MDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSDEKERVACR 60
           MDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSS+EKERVACR
Sbjct: 51  MDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSNEKERVACR 110

Query: 61  REIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSYRWGTPTA 120
           REIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKK TSGILDNSYRWGTPTA
Sbjct: 111 REIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKITSGILDNSYRWGTPTA 170

Query: 121 THPNQFSWSEAFHIPLTKPINELTDLTSWNGREVMEEVASAMSKVARKLAGVLVESMGQR 180
           THPNQ SWSEAFHIPLTK             REVMEEVASAMSKVARKLAGVLVESMGQR
Sbjct: 171 THPNQLSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLVESMGQR 230

Query: 181 KELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQW 240
           KELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQW
Sbjct: 231 KELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQW 290

Query: 241 LAVKPNPQALVVNIGDLLQSLLPI 265
           LAVKPNPQALVVNIGDLLQ   PI
Sbjct: 291 LAVKPNPQALVVNIGDLLQVQTPI 314

[Close]
BLAST of Cucsa.372030 vs. NCBI nr
Match: gi|1021496768|ref|XP_016191436.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Arachis ipaensis])

HSP 1 Score: 319.7 bits (818), Expect = 5.0e-84
Identity = 161/272 (59.19%), Postives = 198/272 (72.79%), Query Frame = 1 

Query: 1   MDSNPPLFDQYEFLLQSTAHLQPFNNGN-------LPTNSVMEECQLPLIDLKGLKSSDE 60
           +DSNPPL   Y+ LL+ +A LQ  NN N       +  + +++EC+LPLIDL GL+S + 
Sbjct: 3   IDSNPPLLHHYQALLRKSAKLQHANNNNNNNRTIEIHNDPIVDECELPLIDLSGLESCNA 62

Query: 61  KERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSY 120
            ERVAC   I +A+ EWGFFQV+NHGI TELL  M +EQ+KLF VPF++K TS IL+N Y
Sbjct: 63  TERVACTAAICKAASEWGFFQVVNHGISTELLRNMKEEQMKLFKVPFDRKVTSEILNNPY 122

Query: 121 RWGTPTATHPNQFSWSEAFHIPLTKPINELTDLTSWNG----REVMEEVASAMSKVARKL 180
           RWGTPTAT  NQFSWSEAFHIPLTK    ++D  SW      RE + E A AM KV+R L
Sbjct: 123 RWGTPTATRSNQFSWSEAFHIPLTK----ISDAASWGEFTSLREAINEFAPAMLKVSRLL 182

Query: 181 AGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFS-GEVSGLVPHTDSDFLTILHQDS 240
           A +L E++G   + +E +CD STCFLRLNHYP CP S  E+ GLVPHTDSDFLTIL+QD 
Sbjct: 183 ASILAENLGYPNDAIEKLCDASTCFLRLNHYPSCPKSQDEIFGLVPHTDSDFLTILYQDQ 242

Query: 241 GGGLQVMKGSQWLAVKPNPQALVVNIGDLLQS 261
            GGLQ+MK S+W+AVKPNP AL+VNIGDL Q+
Sbjct: 243 VGGLQLMKDSKWVAVKPNPDALIVNIGDLFQA 270

[Close]
BLAST of Cucsa.372030 vs. NCBI nr
Match: gi|703159524|ref|XP_010112271.1| (Gibberellin 2-beta-dioxygenase 8 [Morus notabilis])

HSP 1 Score: 318.2 bits (814), Expect = 1.5e-83
Identity = 158/260 (60.77%), Postives = 190/260 (73.08%), Query Frame = 1 

Query: 1   MDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSDEKERVACR 60
           +DSNPPL D Y+ LL  ++ LQ             EECQLP+IDL GLKS +  ERV C 
Sbjct: 3   IDSNPPLLDHYKALLDHSSTLQTAECDGHFDGVAAEECQLPMIDLNGLKSDEALERVECA 62

Query: 61  REIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSYRWGTPTA 120
           + I +A+ EWGFFQV+NHGI  EL+ +M KEQ KLF  PFE K TS +L+NSYRWGTPTA
Sbjct: 63  KAISKAASEWGFFQVVNHGISLELVKKMRKEQTKLFEAPFEDKATSRLLNNSYRWGTPTA 122

Query: 121 THPNQFSWSEAFHIPLTKPINELTDLTSWNGREVMEEVASAMSKVARKLAGVLVESMGQR 180
           T P+QFSWSEAFHIPLTK   E       + RE  EE A+AMSK+AR LAGVL E++G R
Sbjct: 123 TRPSQFSWSEAFHIPLTKVSEEDCYGDFISLREPTEEFAAAMSKLARSLAGVLAENLGHR 182

Query: 181 KELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQW 240
           +++ E+ICDESTCFLRLN YP C  S E+ GLVPHTDS FLTIL QD  GGLQ+MK  +W
Sbjct: 183 RQVFEEICDESTCFLRLNRYPACAVSSEIFGLVPHTDSGFLTILRQDHVGGLQLMKDYKW 242

Query: 241 LAVKPNPQALVVNIGDLLQS 261
           ++VKPNP+AL+VNIGDL Q+
Sbjct: 243 VSVKPNPEALIVNIGDLFQA 262

The following BLAST results are available for this feature:
BLAST of Cucsa.372030 vs. Swiss-Prot
Match NameE-valueIdentityDescription
G2OX8_ARATH4.1e-6247.71Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2[more]
G2OX7_ARATH1.6e-4542.08Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1[more]
GAO1B_WHEAT1.1e-2533.47Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1[more]
GAO1D_WHEAT2.4e-2533.47Gibberellin 20 oxidase 1-D OS=Triticum aestivum GN=GA20ox1D PE=1 SV=1[more]
GAOX1_ARATH6.9e-2532.22Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2[more]
BLAST of Cucsa.372030 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KIW0_CUCSA2.1e-13794.62Uncharacterized protein OS=Cucumis sativus GN=Csa_5G052540 PE=3 SV=1[more]
W9SYI1_9ROSA1.0e-8360.77Gibberellin 2-beta-dioxygenase 8 OS=Morus notabilis GN=L484_014953 PE=3 SV=1[more]
M5XWN4_PRUPE1.9e-8260.45Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008125mg PE=3 SV=1[more]
F6HG07_VITVI7.3e-8260.92Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g01650 PE=3 SV=... [more]
A0A061E9S8_THECC7.3e-8261.00Gibberellin 2-oxidase 8 OS=Theobroma cacao GN=TCM_011301 PE=3 SV=1[more]
BLAST of Cucsa.372030 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G21200.12.3e-6347.71 gibberellin 2-oxidase 8[more]
AT1G50960.18.9e-4742.08 gibberellin 2-oxidase 7[more]
AT3G19000.19.2e-2835.83 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G25420.13.9e-2632.22 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G25300.11.1e-2530.80 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
BLAST of Cucsa.372030 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778698231|ref|XP_004142323.2|3.0e-13794.62PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis sativus][more]
gi|659109414|ref|XP_008454707.1|7.4e-13692.80PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X2 [Cucumis melo][more]
gi|659109410|ref|XP_008454705.1|7.4e-13692.80PREDICTED: gibberellin 2-beta-dioxygenase 8-like isoform X1 [Cucumis melo][more]
gi|1021496768|ref|XP_016191436.1|5.0e-8459.19PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Arachis ipaensis][more]
gi|703159524|ref|XP_010112271.1|1.5e-8360.77Gibberellin 2-beta-dioxygenase 8 [Morus notabilis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR026992DIOX_N
IPR027443IPNS-like
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0016491 oxidoreductase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.372030.1Cucsa.372030.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 194..261
score: 6.3
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 189..275
score: 10
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 40..136
score: 3.0
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 30..260
score: 2.9
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 28..261
score: 4.7E
NoneNo IPR availablePANTHERPTHR10209:SF177SUBFAMILY NOT NAMEDcoord: 28..261
score: 4.7E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 27..260
score: 3.68

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.372030Csa5G052540Cucumber (Chinese Long) v2cgycuB520
Cucsa.372030ClCG07G007000Watermelon (Charleston Gray)cgywcgB635
Cucsa.372030CSPI05G01270Wild cucumber (PI 183967)cgycpiB549
Cucsa.372030Lsi04G011840Bottle gourd (USVL1VR-Ls)cgylsiB602
Cucsa.372030CsaV3_5G015180Cucumber (Chinese Long) v3cgycucB564
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None