Carg18263 (gene) Silver-seed gourd

Overview
NameCarg18263
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionE3 ubiquitin-protein ligase
LocationCucurbita_argyrosperma_scaffold_120 : 282454 .. 285985 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAGTTTTCGGTGGGTGGAGAGGAAGACGGGGAGGACGGATCCAGTACCAGTAGCCCTAGGGCGAAGAGGAAGAGAACCTCCTTTCGCATCAACGCCGTCGTTAATTTTCCCCGGCACAACCAACGGTGTGAAGAAGGGGAAGAGCAGGGTTTCGATTACTGTAAGGAAGAAACTGCGAGCGATGAAGAGCAGAGAAAGCGCCATCGAATTGACGGAATAGAGGAACAGGTTGAAGAGGACGGTACCGAAAACGCACTCTGCTCGGTTAAGTCCTGCTCTAGAAATGTGAAGGAAAATGGCTCCATTTCGATGTTTTTGATCGACCCTGAAGTGTTGGATTGTTCCATTTGTTGTGAACCCTTGAGTGTCCCCATCTTCCAGGTATGTTGTTTTCAATAATCTCCGTCCTGGTTCTGTTCGTAATTAGTTGTGATTTTGTAGTTGACTTTGCTCTGATTCCAAATTGGCTTTTCGTTTGGGGGTTTTCCATAAAGATCAGATAGATCCCAAAATTGAGTTGGGTTGGATTTCGTTCGATTGAACCTTTAATCAAATAAGATGAAATGGAAGCCATTAGTAGTTCGTTCGTCTTGTAAAAACTTAAGAACTGCTGGGTTGATATTTAGCAGAAGCGTTGATATCCTCAATAATCTTCTAGTAATTTAATGCATAATCTATGTATACGTGCTCTGTGTGAGGATGAACCAGATAGGTGGAGTATCATTTCTGTACAAATGTGAGGGTCAAGCAACAATCGATGAACCAAATATACCACCATAACAGTTATGGTTGAGAAACGGTACTCGACCTCATGGATGAATGAGAGATCGTTTGTTGCTTCTCAGACAATGAGATAGAACTGGAACTAAGAACACAACTCTGGAATAAAATCACTTATCAAGTGTGTCCACAAGCTGCTGATAATAGGATGGATCATTGAATGGAGGAGATTTGACCTGTAATTGAAGCTGAATTGACATTGGAGTTTCAAAGCGTTTTGCACCAGTCATGCCTGATTTTTCAAGCAATTAGGTTGATGAAGATATACACTAGAAAATGCTGTATGTATTTCAAGCCCAAGAAACTACTTGAGTTGGCCAGGATCAGAAATCTGAATTTCATAGGTTATTCACCCTTCAGAGTACTAATGGAGTGTTTTTTTAAGTGGGAGTGTTTAGTGATTTCAATAAAAATTGGCAGAGAGAAGGAAAGGTTGGATGGGAATTAGAAAGGAGCAACTTGAGGGTTGATGTTCTGAACAAGATGGATGAAAATCCTTAGCAAATGTGGTCAAAGGATTCATGGGATCAAGAACAGTGTGGATAAGGAATTCCAATGGAAAAATGAAGATGAAGAAAAGGATTGCTATTATGAGAGTTGAGTTTGCTAAAATTGATTGGCACGTACCATTGTGGTCAAGAGACCTCAGTTTCATCATGATTCGTTCACCATCGTTGTGGCACTAAGACAGGTGATCTATGAATATTTTATGTGAATCCTTTTGATGCAAAGGAAGCTTTGATTAGAGGAGTTAAGAAAATGGCTTGTACTTGTAAGATTGATGGATTGGCGGATATGAGGAGATTTTAAAGAAGGGGAAAATTTGTGGGGTTTTATTAACTGTTAACACGCTTGATTTCTTAGAAGCATATTTAGAGATAATAGGTAATTTTTGTGGGTTTATTCCAGCCAAATTTGAGATTTAACATCCAAGTGAACGGAGATTTGTCGTGCAAGCTGTGACCTAGCCATTCTTCGAAACTTTTGGCTAGTAGTGTGAGCAAAATTTCCAACAAAGTTGTTTGGATGATGTTGTTTAGAATGTTCATAACAACTTCATAGTATTGCTTGATTGAGATAGAAGTTGAAGATAAATTCAGCTACTTTGTTTTGCTGTTCAAGGGTTTTTTCTTTGACTTTAAATCATGCCCATAGGATCTTTCTTCGTGCGGTTAATGCACGGAAGAAAGTGTATTTAGTATGGAGGACATTAATAAGTGTATGAATAAGAATCTCCTCAGCCTCAATGTCATCTGAGACCAACTCATTCAACTGTAATTTCTGTAAGATCTTGAACATCTAAAATGTGAGATAATGAAGCTGACTGTTTTAGCAGATTGAAAATTTTCCTCGAAACAGAAAATCGAATAGATTCTTGCAGATGCATTCAACCCTTGTTATTAGTCGATGTAACAGTCTAGAAGGATTTGTATATAAGACTGCAGCTCTTATTGGTTTTTTTAATGTATGATTTGTGCATTAGAAGTAAAAGAAAGAAAAAAAAGTCTTCTCATAATTAAATTTCGATCGATCAGTTAACATATGCTTATTCCTGTTGAATTGTTCAGTTTTGGTGTAAACTGATTGCTCGTGCTCGCTCTTTAACCTCTGCTAATTATTTCAGCTATATTTCACATTTCTACTTGTGTTTCAATAGATAAAGTGGTCATACAGTCGGCACGAGCTAGACATGATCGTCTAACAATTAACTGTTTGGTTTTGTCATATCATCAATATATCAACAGTTTGACTAGATTTTTTTATCATGAATTCAGTGTGAGAATGGGCATATTGCATGCTCTTCCTGCTGCAACAAACTTAAGAATAGATGTGCAACCTGCTCTTGGCCTATCGGCTATAATCGTTGTCGGGCCGTCGAGAAGGTTCTTGAATCCATTAAACTACCCTGCAAACAATCAAAGTACGGATGCAAGGAGATGGTTAGTTATGGTAGTGAAAGTGACCACGAAAAGAAATGCCTACATGCCCCTTGTAAATGCCCTCTTCCTGATTGCAACTTTGTTGCCTCATCTAAGCAATTATCACTACATTTCAGCAGTAAACATACAGACTCTGCTATCAGCTTTCAGTTCAGTTCTAGCTTCACCATTTGCCTAAACGCTAACGATAATTACCGCGTTCTTCAAGAACAAGGTGATGGTATTCTTTTTATTCTCAGCAATAGTTTTGATCATCTGGGAAATGCTGTTAAAGTTTGCTGCCTTAGACCTCCTTCCTTGAAGGAAGCATTTTCTTATGATCTACGAGTAGAAACTCAAGATCTCTCCCTAGTTTTACAATCTTCCATGAAGAACATTCAATCATCATCAGATTGCTGTCCCTCGACGAGGTTCCTTTTGATTCCACGCGATTTGTTCAGTTCTTCAGGCATGATCAAGTTGAATATCTGCATATGGCAAAACACCGGTGCACCACCGTAGATAAAAGGCGTCGGTAAGTCAACAAGTAAATATCGTGCTGTTTATATAAATGTATATGTCAGTGTGCATATTGTGTACATGTCATGGAAAATAAGGTCAGTCTTAGAAGCTCAGTTATGCTGCATAATTTGTATATAGCAGCAGTTTCCGATTTCGTAAGTTTATTCTAACTCGTTCGGTCCATGGTCTGGGAACTCGGTTGTGTTATATTACCACTACTTAAATGAACTCTTTCAGATGGACCCTGTTCGTGTTTGAAGTTTCTTTTGATTTTTTTGTTCATGTTATCCAAAATGTTCATGAAC

mRNA sequence

ATGGCGAAGTTTTCGGTGGGTGGAGAGGAAGACGGGGAGGACGGATCCAGTACCAGTAGCCCTAGGGCGAAGAGGAAGAGAACCTCCTTTCGCATCAACGCCGTCGTTAATTTTCCCCGGCACAACCAACGGTGTGAAGAAGGGGAAGAGCAGGGTTTCGATTACTGTAAGGAAGAAACTGCGAGCGATGAAGAGCAGAGAAAGCGCCATCGAATTGACGGAATAGAGGAACAGGTTGAAGAGGACGGTACCGAAAACGCACTCTGCTCGGTTAAGTCCTGCTCTAGAAATGTGAAGGAAAATGGCTCCATTTCGATGTTTTTGATCGACCCTGAAGTGTTGGATTGTTCCATTTGTTGTGAACCCTTGAGTGTCCCCATCTTCCAGAGACCTCAGTTTCATCATGATTCGTTCACCATCGTTGTGGCACTAAGACAGTGTGAGAATGGGCATATTGCATGCTCTTCCTGCTGCAACAAACTTAAGAATAGATGTGCAACCTGCTCTTGGCCTATCGGCTATAATCGTTGTCGGGCCGTCGAGAAGGTTCTTGAATCCATTAAACTACCCTGCAAACAATCAAAGTACGGATGCAAGGAGATGGTTAGTTATGGTAGTGAAAGTGACCACGAAAAGAAATGCCTACATGCCCCTTGTAAATGCCCTCTTCCTGATTGCAACTTTGTTGCCTCATCTAAGCAATTATCACTACATTTCAGCAGTAAACATACAGACTCTGCTATCAGCTTTCAGTTCAGTTCTAGCTTCACCATTTGCCTAAACGCTAACGATAATTACCGCGTTCTTCAAGAACAAGGTGATGGTATTCTTTTTATTCTCAGCAATAGTTTTGATCATCTGGGAAATGCTGTTAAAGTTTGCTGCCTTAGACCTCCTTCCTTGAAGGAAGCATTTTCTTATGATCTACGAGTAGAAACTCAAGATCTCTCCCTAGTTTTACAATCTTCCATGAAGAACATTCAATCATCATCAGATTGCTGTCCCTCGACGAGGTTCCTTTTGATTCCACGCGATTTGTTCAGTTCTTCAGGCATGATCAAGTTGAATATCTGCATATGGCAAAACACCGGTGCACCACCGTAGATAAAAGGCGTCGGTAAGTCAACAAGTAAATATCGTGCTGTTTATATAAATGTATATGTCAGTGTGCATATTGTGTACATGTCATGGAAAATAAGGTCAGTCTTAGAAGCTCAGTTATGCTGCATAATTTGTATATAGCAGCAGTTTCCGATTTCGTAAGTTTATTCTAACTCGTTCGGTCCATGGTCTGGGAACTCGGTTGTGTTATATTACCACTACTTAAATGAACTCTTTCAGATGGACCCTGTTCGTGTTTGAAGTTTCTTTTGATTTTTTTGTTCATGTTATCCAAAATGTTCATGAAC

Coding sequence (CDS)

ATGGCGAAGTTTTCGGTGGGTGGAGAGGAAGACGGGGAGGACGGATCCAGTACCAGTAGCCCTAGGGCGAAGAGGAAGAGAACCTCCTTTCGCATCAACGCCGTCGTTAATTTTCCCCGGCACAACCAACGGTGTGAAGAAGGGGAAGAGCAGGGTTTCGATTACTGTAAGGAAGAAACTGCGAGCGATGAAGAGCAGAGAAAGCGCCATCGAATTGACGGAATAGAGGAACAGGTTGAAGAGGACGGTACCGAAAACGCACTCTGCTCGGTTAAGTCCTGCTCTAGAAATGTGAAGGAAAATGGCTCCATTTCGATGTTTTTGATCGACCCTGAAGTGTTGGATTGTTCCATTTGTTGTGAACCCTTGAGTGTCCCCATCTTCCAGAGACCTCAGTTTCATCATGATTCGTTCACCATCGTTGTGGCACTAAGACAGTGTGAGAATGGGCATATTGCATGCTCTTCCTGCTGCAACAAACTTAAGAATAGATGTGCAACCTGCTCTTGGCCTATCGGCTATAATCGTTGTCGGGCCGTCGAGAAGGTTCTTGAATCCATTAAACTACCCTGCAAACAATCAAAGTACGGATGCAAGGAGATGGTTAGTTATGGTAGTGAAAGTGACCACGAAAAGAAATGCCTACATGCCCCTTGTAAATGCCCTCTTCCTGATTGCAACTTTGTTGCCTCATCTAAGCAATTATCACTACATTTCAGCAGTAAACATACAGACTCTGCTATCAGCTTTCAGTTCAGTTCTAGCTTCACCATTTGCCTAAACGCTAACGATAATTACCGCGTTCTTCAAGAACAAGGTGATGGTATTCTTTTTATTCTCAGCAATAGTTTTGATCATCTGGGAAATGCTGTTAAAGTTTGCTGCCTTAGACCTCCTTCCTTGAAGGAAGCATTTTCTTATGATCTACGAGTAGAAACTCAAGATCTCTCCCTAGTTTTACAATCTTCCATGAAGAACATTCAATCATCATCAGATTGCTGTCCCTCGACGAGGTTCCTTTTGATTCCACGCGATTTGTTCAGTTCTTCAGGCATGATCAAGTTGAATATCTGCATATGGCAAAACACCGGTGCACCACCGTAG

Protein sequence

MAKFSVGGEEDGEDGSSTSSPRAKRKRTSFRINAVVNFPRHNQRCEEGEEQGFDYCKEETASDEEQRKRHRIDGIEEQVEEDGTENALCSVKSCSRNVKENGSISMFLIDPEVLDCSICCEPLSVPIFQRPQFHHDSFTIVVALRQCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAVEKVLESIKLPCKQSKYGCKEMVSYGSESDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFSSKHTDSAISFQFSSSFTICLNANDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRPPSLKEAFSYDLRVETQDLSLVLQSSMKNIQSSSDCCPSTRFLLIPRDLFSSSGMIKLNICIWQNTGAPP
Homology
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BLAST of Carg18263 vs. NCBI nr
Match: XP_022956630.1 (E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita moschata])

HSP 1 Score: 708.4 bits (1827), Expect = 1.3e-200
Identity = 350/367 (95.37%), Postives = 350/367 (95.37%), Query Frame = 0 

Query: 1   MAKFSVGGEEDGEDGSSTSSPRAKRKRTSFRINAVVNFPRHNQRCEEGEEQGFDYCKEET 60
           MAKFSVGGEEDGEDGSSTSSPRAKRKRTSFRINAVVNFPRHNQRCEEGEEQGFDYCKEET
Sbjct: 1   MAKFSVGGEEDGEDGSSTSSPRAKRKRTSFRINAVVNFPRHNQRCEEGEEQGFDYCKEET 60

Query: 61  ASDEEQRKRHRIDGIEEQVEEDGTENALCSVKSCSRNVKENGSISMFLIDPEVLDCSICC 120
           ASDEEQRKRHRIDGIEEQVEEDGTENALCSVKSCSRNVKENGSISMFLIDPEVLDCSICC
Sbjct: 61  ASDEEQRKRHRIDGIEEQVEEDGTENALCSVKSCSRNVKENGSISMFLIDPEVLDCSICC 120

Query: 121 EPLSVPIFQRPQFHHDSFTIVVALRQCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAV 180
           EPLSVPIF                 QCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAV
Sbjct: 121 EPLSVPIF-----------------QCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAV 180

Query: 181 EKVLESIKLPCKQSKYGCKEMVSYGSESDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFS 240
           EKVLESIKLPCKQSKYGCKEMVSYGSESDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFS
Sbjct: 181 EKVLESIKLPCKQSKYGCKEMVSYGSESDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFS 240

Query: 241 SKHTDSAISFQFSSSFTICLNANDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRPPS 300
           SKHTDSAISFQFSSSFTICLNANDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRPPS
Sbjct: 241 SKHTDSAISFQFSSSFTICLNANDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRPPS 300

Query: 301 LKEAFSYDLRVETQDLSLVLQSSMKNIQSSSDCCPSTRFLLIPRDLFSSSGMIKLNICIW 360
           LKEAFSYDLRVETQDLSLVLQSSMKNIQSSSDCCPSTRFLLIPRDLFSSSGMIKLNICIW
Sbjct: 301 LKEAFSYDLRVETQDLSLVLQSSMKNIQSSSDCCPSTRFLLIPRDLFSSSGMIKLNICIW 350

Query: 361 QNTGAPP 368
           QNTGAPP
Sbjct: 361 QNTGAPP 350

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BLAST of Carg18263 vs. NCBI nr
Match: XP_023534163.1 (E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 695.3 bits (1793), Expect = 1.1e-196
Identity = 344/367 (93.73%), Postives = 346/367 (94.28%), Query Frame = 0 

Query: 1   MAKFSVGGEEDGEDGSSTSSPRAKRKRTSFRINAVVNFPRHNQRCEEGEEQGFDYCKEET 60
           MAKFSVGGEEDGEDGSSTSSPRAKRKRTSFRINAVVNFPRHNQRCEEGEEQGFDYCKEET
Sbjct: 30  MAKFSVGGEEDGEDGSSTSSPRAKRKRTSFRINAVVNFPRHNQRCEEGEEQGFDYCKEET 89

Query: 61  ASDEEQRKRHRIDGIEEQVEEDGTENALCSVKSCSRNVKENGSISMFLIDPEVLDCSICC 120
           ASDEEQRKRHRIDGIEEQV EDGTE ALCSVKSCS+NVKENGSISMFLIDPEVLDCSICC
Sbjct: 90  ASDEEQRKRHRIDGIEEQVGEDGTEKALCSVKSCSKNVKENGSISMFLIDPEVLDCSICC 149

Query: 121 EPLSVPIFQRPQFHHDSFTIVVALRQCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAV 180
           EPLSVPIF                 QCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAV
Sbjct: 150 EPLSVPIF-----------------QCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAV 209

Query: 181 EKVLESIKLPCKQSKYGCKEMVSYGSESDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFS 240
           EKVLESIKLPCKQSKYGCKE+VSYGSESDHEKKCLHAPCKCPL DCNFVASSKQLSLHFS
Sbjct: 210 EKVLESIKLPCKQSKYGCKEIVSYGSESDHEKKCLHAPCKCPLSDCNFVASSKQLSLHFS 269

Query: 241 SKHTDSAISFQFSSSFTICLNANDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRPPS 300
           SKHTDSAISFQFSSSFTICLNANDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRPPS
Sbjct: 270 SKHTDSAISFQFSSSFTICLNANDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRPPS 329

Query: 301 LKEAFSYDLRVETQDLSLVLQSSMKNIQSSSDCCPSTRFLLIPRDLFSSSGMIKLNICIW 360
           LKEAFSYDLRVETQDLSLVLQSSMKNIQSSSDCCPSTRFLLIPRDLFSSSG IKLNICIW
Sbjct: 330 LKEAFSYDLRVETQDLSLVLQSSMKNIQSSSDCCPSTRFLLIPRDLFSSSGKIKLNICIW 379

Query: 361 QNTGAPP 368
           QNTGAPP
Sbjct: 390 QNTGAPP 379

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BLAST of Carg18263 vs. NCBI nr
Match: XP_022990964.1 (E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita maxima])

HSP 1 Score: 665.6 bits (1716), Expect = 9.6e-188
Identity = 331/367 (90.19%), Postives = 339/367 (92.37%), Query Frame = 0 

Query: 1   MAKFSVGGEEDGEDGSSTSSPRAKRKRTSFRINAVVNFPRHNQRCEEGEEQGFDYCKEET 60
           MAKFSVGGEEDGEDGSSTSSPRAKRKRTSFRINAVVNFPRHN++ EEGEEQGFDYCKEET
Sbjct: 1   MAKFSVGGEEDGEDGSSTSSPRAKRKRTSFRINAVVNFPRHNRQREEGEEQGFDYCKEET 60

Query: 61  ASDEEQRKRHRIDGIEEQVEEDGTENALCSVKSCSRNVKENGSISMFLIDPEVLDCSICC 120
           ASDEEQRKRHRID IEEQV EDGTE ALCSVKSCS+NVKENGSISMFLIDPEVLDCSICC
Sbjct: 61  ASDEEQRKRHRIDEIEEQVGEDGTEKALCSVKSCSKNVKENGSISMFLIDPEVLDCSICC 120

Query: 121 EPLSVPIFQRPQFHHDSFTIVVALRQCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAV 180
           EPLSVPIF                 QCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAV
Sbjct: 121 EPLSVPIF-----------------QCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAV 180

Query: 181 EKVLESIKLPCKQSKYGCKEMVSYGSESDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFS 240
           EKVLESI+LPCKQSKYGCKE+VSYGSESDH+KKCLHAPCKCPL DCNFVASSKQLSLHFS
Sbjct: 181 EKVLESIRLPCKQSKYGCKEIVSYGSESDHDKKCLHAPCKCPLSDCNFVASSKQLSLHFS 240

Query: 241 SKHTDSAISFQFSSSFTICLNANDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRPPS 300
           SKHTDSAISFQFSSSFTICLNANDNY VLQEQGDGILFILSNSFDHLGNAVKVCC+RPPS
Sbjct: 241 SKHTDSAISFQFSSSFTICLNANDNYCVLQEQGDGILFILSNSFDHLGNAVKVCCIRPPS 300

Query: 301 LKEAFSYDLRVETQDLSLVLQSSMKNIQSSSDCCPSTRFLLIPRDLFSSSGMIKLNICIW 360
           LKEAFSYDLRVETQDLSLVLQSSMKNI+SSSDC PSTRFLLIPRDLFSSS  IKLNICIW
Sbjct: 301 LKEAFSYDLRVETQDLSLVLQSSMKNIRSSSDCYPSTRFLLIPRDLFSSSSKIKLNICIW 350

Query: 361 QNTGAPP 368
           QNTG  P
Sbjct: 361 QNTGGAP 350

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BLAST of Carg18263 vs. NCBI nr
Match: XP_008460511.1 (PREDICTED: E3 ubiquitin-protein ligase SINA-like 7 [Cucumis melo])

HSP 1 Score: 522.7 bits (1345), Expect = 1.0e-144
Identity = 262/366 (71.58%), Postives = 294/366 (80.33%), Query Frame = 0 

Query: 1   MAKFSVGGEEDGEDGSSTSSPRAKRKRTSFRINAVVNFPRHNQRCEEGEEQGFDYCKEET 60
           MAKFSV GEED  DG   SSPR KRKR+SFRINA V+FPR +++ E+GEEQGF+  K   
Sbjct: 1   MAKFSVDGEEDAGDG--PSSPRGKRKRSSFRINATVDFPRRSEQREQGEEQGFNSDKXXX 60

Query: 61  ASDEEQRKRHRIDGIEEQVEEDGTENALCSVKSCSRNVKENGSISMFLIDPEVLDCSICC 120
                     R D IEE + EDG + AL S KSC++N K NGS+SMFLIDPEVLDCSICC
Sbjct: 61  XXXXXXXXXXRNDEIEEHLREDGGKRALWSGKSCTKNAKPNGSVSMFLIDPEVLDCSICC 120

Query: 121 EPLSVPIFQRPQFHHDSFTIVVALRQCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAV 180
           EPLS+PIF                 QCENGHIACSSCCNKLKN+CATCSWPIGYNRCRAV
Sbjct: 121 EPLSIPIF-----------------QCENGHIACSSCCNKLKNKCATCSWPIGYNRCRAV 180

Query: 181 EKVLESIKLPCKQSKYGCKEMVSYGSESDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFS 240
           EKVLESIKLPCKQSK+GCKE++ YG+ESDHEK CL+APC CP  DC F+AS+KQLSLHFS
Sbjct: 181 EKVLESIKLPCKQSKFGCKEIIGYGNESDHEKTCLYAPCLCPYSDCKFIASAKQLSLHFS 240

Query: 241 SKHTDSAISFQFSSSFTICLNANDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRPPS 300
           +KHT+SA +F FSSSFTICL  ND Y VLQEQ DG+LFILSNS ++LG+ VK+CCL+PPS
Sbjct: 241 NKHTNSATNFHFSSSFTICLKTNDTYHVLQEQ-DGVLFILSNSSENLGSVVKICCLQPPS 300

Query: 301 LKEAFSYDLRVETQDLSLVLQSSMKNIQSSSDCCPSTRFLLIPRDLFSSSGMIKLNICIW 360
           LKE FSYDLR ETQDLSLVLQSSMKNIQSSSDCC STRFL+IPRDLFSSSGMIKLNICIW
Sbjct: 301 LKETFSYDLRAETQDLSLVLQSSMKNIQSSSDCCHSTRFLMIPRDLFSSSGMIKLNICIW 346

Query: 361 QNTGAP 367
           QN  +P
Sbjct: 361 QNGSSP 346

[Close]
BLAST of Carg18263 vs. NCBI nr
Match: XP_022141698.1 (E3 ubiquitin-protein ligase SINA-like 10 [Momordica charantia])

HSP 1 Score: 454.5 bits (1168), Expect = 3.4e-124
Identity = 238/369 (64.50%), Postives = 274/369 (74.25%), Query Frame = 0 

Query: 1   MAKFSVGGEEDGEDGSSTSSPRAKRKRTSFRINAVVNFPRHNQRCEEGEEQGFDYCKEET 60
           MAKFSVGGE+DGEDG   SSP  KR+R S RINA V++P  N+       + FD  KE+T
Sbjct: 29  MAKFSVGGEDDGEDG-GPSSPTGKRRRPSSRINATVDYPHQNE-----PREAFDSDKEQT 88

Query: 61  ASDE-EQRKRHRIDGIEEQVEEDGTENALCSVKSCSRNVKEN--GSISMFLIDPEVLDCS 120
            ++E +QRKRHRI  I +QV E  T+ AL SV SCS+ +K N  GS+ MFLIDPEVLDCS
Sbjct: 89  ETEELDQRKRHRIHEIHQQVPEGATKKALWSVTSCSKTLKTNGGGSVPMFLIDPEVLDCS 148

Query: 121 ICCEPLSVPIFQRPQFHHDSFTIVVALRQCENGHIACSSCCNKLKNRCATCSWPIGYNRC 180
           ICCEPL+                                     KN+CA+CSWPIGYNRC
Sbjct: 149 ICCEPLT-----------------XXXXXXXXXXXXXXXXXXXXKNKCASCSWPIGYNRC 208

Query: 181 RAVEKVLESIKLPCKQSKYGCKEMVSYGSESDHEKKCLHAPCKCPLPDCNFVASSKQLSL 240
           RAVEKVLESIKLPCKQS+YGCKEMVSYG+ES+HEK CL+APC CPL DCNF ASSKQLSL
Sbjct: 209 RAVEKVLESIKLPCKQSQYGCKEMVSYGNESEHEKTCLYAPCLCPLSDCNFTASSKQLSL 268

Query: 241 HFSSKHTDSAISFQFSSSFTICLNANDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLR 300
           HFSSKH +SA +FQFSSSFTICL  +D+Y +LQEQ DGILFILSNSF+HLG+AVKVCCLR
Sbjct: 269 HFSSKHKNSATNFQFSSSFTICLKTDDSYHILQEQDDGILFILSNSFEHLGSAVKVCCLR 328

Query: 301 PPSLKEAFSYDLRVETQDLSLVLQSSMKNIQSSSDCCPSTRFLLIPRDLFSSSGMIKLNI 360
           P  LKEAF+YDL+ ETQDLSL L SSMKNI+SSSDCC STRFLLIP DLF S G IKL+I
Sbjct: 329 PNFLKEAFTYDLKAETQDLSLFLHSSMKNIKSSSDCCHSTRFLLIPSDLFDSRGQIKLDI 374

Query: 361 CIWQNTGAP 367
           CIW++  +P
Sbjct: 389 CIWRDGSSP 374

[Close]
BLAST of Carg18263 vs. TAIR10
Match: AT5G37930.1 (Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 203.0 bits (515), Expect = 3.2e-52
Identity = 110/276 (39.86%), Postives = 156/276 (56.52%), Query Frame = 0 

Query: 91  VKSCSRNVKENGSISMFLIDPEVLDCSICCEPLSVPIFQRPQFHHDSFTIVVALRQCENG 150
           V S S +  ++  +S+ L+DP+VLDC ICCEPL +PIF                 QC+NG
Sbjct: 88  VNSQSSSSSKDSPLSVTLLDPDVLDCPICCEPLKIPIF-----------------QCDNG 147

Query: 151 HIACSSCCNKLKNRCATCSWPIGYNRCRAVEKVLESIKLPCKQSKYGCKEMVSYGSESDH 210
           H+AC+ CC K++NRC +C+ PIGY RCRA+EKV+E+ ++ C  +KYGCKE  SYG+   H
Sbjct: 148 HLACTLCCTKVRNRCPSCTLPIGYVRCRAMEKVIEASRVSCLNAKYGCKESTSYGNRFSH 207

Query: 211 EKKCLHAPCKCPLPDCNFVASSKQLSLHFSSKHTDSAISFQFSSSFTICLNANDNYRVLQ 270
           E+ C+  PC CP+ DC++    K L+ H  ++H D  ISF +++  TI L+ N    +LQ
Sbjct: 208 EQVCVFTPCSCPILDCHYTGYYKDLNNHVRAEHKDDLISFVWNTRLTISLDLNKKTTILQ 267

Query: 271 EQGDGILFILSNSFDHLGNAVKVCCLRP--PSLKEAFSYDLRVETQDLSLVLQSSMKNIQ 330
           E+ DG + ++          V V C+ P  P +    S  L   T D  L     +KNIQ
Sbjct: 268 EENDGHVIVVQVFRALHAVYVSVSCIAPLTPGVGR-LSCRLAKITVDSLLKQGFMVKNIQ 327

Query: 331 SSSDCCPSTRFLLIPRDLFSSSGMIKLNICIWQNTG 365
             ++  P   F+LIP  LF  SG   LN+ IW   G
Sbjct: 328 KVTNEHPEDGFMLIPSYLF--SGNDNLNLQIWIGHG 343

[Close]
BLAST of Carg18263 vs. TAIR10
Match: AT5G37890.1 (Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 168.3 bits (425), Expect = 8.7e-42
Identity = 96/263 (36.50%), Postives = 137/263 (52.09%), Query Frame = 0 

Query: 88  LCSVKSCSRNVKENGSISMFLIDPEVLDCSICCEPLSVPIFQRPQFHHDSFTIVVALRQC 147
           L S  +  R+ K+    S  L+D E+LDC IC E  ++PIF                 QC
Sbjct: 26  LSSSDAAKRDAKKR---STMLMDLEILDCPICYEAFTIPIF-----------------QC 85

Query: 148 ENGHIACSSCCNKLKNRCATCSWPIGYNRCRAVEKVLESIKLPCKQSKYGCKEMVSYGSE 207
           +NGH+ACSSCC KL N+C  C+ P+G+NRCRA+E VLESI +PC  +K GCK+ VSYG E
Sbjct: 86  DNGHLACSSCCPKLNNKCPACTSPVGHNRCRAMESVLESILIPCPNAKLGCKKNVSYGKE 145

Query: 208 SDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFSSKHTD--SAISFQFSSSFTICLNANDN 267
             HEK+C+ + C CP  DCN+ +S K L  H+   H +     +F      ++ +N +  
Sbjct: 146 LTHEKECMFSHCACPALDCNYTSSYKDLYTHYRITHMEINQINTFICDIPLSVRMNISKK 205

Query: 268 YRVLQEQGDGILFILSNSFDHLGNAVKVCCLRPPSLK-EAFSYDLRVETQDLSLVLQS-S 327
             +  E     LF +    +  G  V V C+ P S +   +SY L       +++ QS  
Sbjct: 206 ILIRTEHLTNHLFAVQCFREPYGVYVTVSCIAPSSPELSQYSYALSYTVDGHTVIYQSPE 265

Query: 328 MKNIQSSSDCCPSTRFLLIPRDL 347
           +K +   S   P   F+LIP  L
Sbjct: 266 VKRVLKLSFQTPQENFMLIPNSL 268

[Close]
BLAST of Carg18263 vs. TAIR10
Match: AT5G37870.1 (Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 163.3 bits (412), Expect = 2.8e-40
Identity = 95/287 (33.10%), Postives = 151/287 (52.61%), Query Frame = 0 

Query: 80  EEDGTENALC-SVKSCSRNVKENGSISMFLIDPEVLDCSICCEPLSVPIFQRPQFHHDSF 139
           E +G++   C S+ S     + + + S  L D ++LDC IC + L +P+F          
Sbjct: 8   ERNGSQKRHCSSISSDDGRKRVDKTRSAMLTDLDILDCPICYQALKIPVF---------- 67

Query: 140 TIVVALRQCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAVEKVLESIKLPCKQSKYGC 199
                  QC NGH+ACSSCC KL+N+C  C+ P+G+ RCRA+E+VLES+ +PC+ +  GC
Sbjct: 68  -------QCGNGHLACSSCCPKLRNKCPACALPVGHIRCRAMERVLESVLVPCRYADLGC 127

Query: 200 KEMVSYGSESDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFSSKHTDSAISFQFS--SSF 259
            + + YG ES HEK C  +PC CP+  CN+  S K L  H+   H+  + ++ F+  S  
Sbjct: 128 TKTIYYGRESTHEKICNFSPCSCPVQGCNYTGSYKDLYEHYDLTHSTGSTAYSFNGVSYI 187

Query: 260 TICLNANDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRP--PSLKEAFSYDLRVET- 319
              +  +D   + +     +LF++    +  G  V V C+ P  P + E FSY L   T 
Sbjct: 188 AAMMFISDKILIERVYEKKLLFVVQCFEEPCGVYVSVSCIAPSAPEVGE-FSYGLLYTTW 247

Query: 320 QDLSLVLQS-SMKNIQSSSDCCPSTRFLLIPRDLFSSSGMIKLNICI 360
           + +++  QS  +K +   S   P   F+LIP  L     ++ + +CI
Sbjct: 248 EGVTMTYQSPKVKKVLKVSSQRPKDSFMLIPHSLLCGP-LLGMMLCI 275

[Close]
BLAST of Carg18263 vs. TAIR10
Match: AT5G37910.1 (Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 153.3 bits (386), Expect = 2.9e-37
Identity = 90/283 (31.80%), Postives = 141/283 (49.82%), Query Frame = 0 

Query: 87  ALCSVKSCSRNVKENGSISMFLIDPEVLDCSICCEPLSVPIFQRPQFHHDSFTIVVALRQ 146
           AL S       V +    ++ L+D ++LDC ICCE L+ PIF                 Q
Sbjct: 9   ALISQGDGGERVAKRQRSAIVLLDLDILDCPICCEALTSPIF-----------------Q 68

Query: 147 CENGHIACSSCCNKLKNRCATCSWPIGYNRCRAVEKVLESIKLPCKQSKYGCKEMVSYGS 206
           C+NGH+AC SCC KL N+C  C+ P+G++R RA+E VLESI +PC   ++GC +   YG 
Sbjct: 69  CDNGHLACGSCCPKLSNKCPACTLPVGHSRSRAMESVLESILIPCPNVRFGCTKSFFYGK 128

Query: 207 ESDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFSSKHTDSAI----SFQFSSSFTICLNA 266
           ES HEK+C+ + C CP   C++  S K L  H+   H+ +       F+ ++ FT  +  
Sbjct: 129 ESAHEKECIFSQCSCPSSVCDYTGSYKDLYAHYKLTHSTNIFWNIKRFRCANFFTTSMLI 188

Query: 267 NDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRP--PSLKEAFSYDLRVETQDLSLVL 326
           +D   + +     +L  +    +  G  V V  + P  P + E FSY L       ++  
Sbjct: 189 SDKILIKRVHEKKLLLAVQCFREPCGVYVTVSFIAPSAPEVGE-FSYQLSYNVDGHTVTY 248

Query: 327 QS-SMKNIQSSSDCCPSTRFLLIPRDLFSSSGMIKLNICIWQN 363
           +S  +K +   S   P   F+LIP  L     ++++ + I +N
Sbjct: 249 ESPEVKRVCKVSIETPQENFMLIPHSLLRGD-LLEMQVFIIEN 272

[Close]
BLAST of Carg18263 vs. TAIR10
Match: AT1G66620.1 (Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 151.4 bits (381), Expect = 1.1e-36
Identity = 100/299 (33.44%), Postives = 138/299 (46.15%), Query Frame = 0 

Query: 66  QRKRHRIDGIEEQVEEDGTENALCSVKSCSRNVKENGSISMFLIDPEVLDCSICCEPLSV 125
           +RKR R+    E VE  G +            V  +G+    L + ++LDC ICC  L+ 
Sbjct: 9   RRKRQRVPSSVESVENGGGD-----------AVARSGT----LFELDLLDCPICCHALTS 68

Query: 126 PIFQRPQFHHDSFTIVVALRQCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAVEKVLE 185
           PIF                 QC+NGHIACSSCC KL+N+C +C+ PIG  R R +E+V+E
Sbjct: 69  PIF-----------------QCDNGHIACSSCCTKLRNKCPSCALPIGNFRSRIMERVVE 128

Query: 186 SIKLPCKQSKYGCKEMVSYGSESDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFSSKHTD 245
           ++ + C   K+GC E  SYG E  HEK C  A C CP P+CN+    K L  HF   H D
Sbjct: 129 AVMVTCPNVKHGCTEKFSYGKELIHEKDCRFALCYCPAPNCNYSGVYKDLYSHFYVNHYD 188

Query: 246 SAISFQFSSSFTICLNANDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRP--PSLKE 305
           +       +     L  ++   VLQ  G G L  +    +  G  V V C+ P  P + E
Sbjct: 189 TWNQIGCGNFAGAWLRISEKILVLQ-YGQGPLIAVQCFKETQGMYVTVNCIAPCAPGVGE 248

Query: 306 ---AFSYDLRVETQDLSLVLQSSMKNIQSSSDCCPSTRFLLIPRDLFSSSGMIKLNICI 360
                SY + +      +     M  IQ  S   P   F+L+P         +K+ ICI
Sbjct: 249 LSFELSYKMPMGGNSTMMFKSEEMNRIQKVSFQTPEKDFMLVPYYFLGDFSTLKMEICI 274

[Close]
BLAST of Carg18263 vs. Swiss-Prot
Match: sp|Q84K34|SIL10_ARATH (E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g37930 PE=2 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 5.8e-51
Identity = 110/276 (39.86%), Postives = 156/276 (56.52%), Query Frame = 0 

Query: 91  VKSCSRNVKENGSISMFLIDPEVLDCSICCEPLSVPIFQRPQFHHDSFTIVVALRQCENG 150
           V S S +  ++  +S+ L+DP+VLDC ICCEPL +PIF                 QC+NG
Sbjct: 88  VNSQSSSSSKDSPLSVTLLDPDVLDCPICCEPLKIPIF-----------------QCDNG 147

Query: 151 HIACSSCCNKLKNRCATCSWPIGYNRCRAVEKVLESIKLPCKQSKYGCKEMVSYGSESDH 210
           H+AC+ CC K++NRC +C+ PIGY RCRA+EKV+E+ ++ C  +KYGCKE  SYG+   H
Sbjct: 148 HLACTLCCTKVRNRCPSCTLPIGYVRCRAMEKVIEASRVSCLNAKYGCKESTSYGNRFSH 207

Query: 211 EKKCLHAPCKCPLPDCNFVASSKQLSLHFSSKHTDSAISFQFSSSFTICLNANDNYRVLQ 270
           E+ C+  PC CP+ DC++    K L+ H  ++H D  ISF +++  TI L+ N    +LQ
Sbjct: 208 EQVCVFTPCSCPILDCHYTGYYKDLNNHVRAEHKDDLISFVWNTRLTISLDLNKKTTILQ 267

Query: 271 EQGDGILFILSNSFDHLGNAVKVCCLRP--PSLKEAFSYDLRVETQDLSLVLQSSMKNIQ 330
           E+ DG + ++          V V C+ P  P +    S  L   T D  L     +KNIQ
Sbjct: 268 EENDGHVIVVQVFRALHAVYVSVSCIAPLTPGVGR-LSCRLAKITVDSLLKQGFMVKNIQ 327

Query: 331 SSSDCCPSTRFLLIPRDLFSSSGMIKLNICIWQNTG 365
             ++  P   F+LIP  LF  SG   LN+ IW   G
Sbjct: 328 KVTNEHPEDGFMLIPSYLF--SGNDNLNLQIWIGHG 343

[Close]
BLAST of Carg18263 vs. Swiss-Prot
Match: sp|Q9FKD7|SINL7_ARATH (E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g37890 PE=2 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.6e-40
Identity = 96/263 (36.50%), Postives = 137/263 (52.09%), Query Frame = 0 

Query: 88  LCSVKSCSRNVKENGSISMFLIDPEVLDCSICCEPLSVPIFQRPQFHHDSFTIVVALRQC 147
           L S  +  R+ K+    S  L+D E+LDC IC E  ++PIF                 QC
Sbjct: 26  LSSSDAAKRDAKKR---STMLMDLEILDCPICYEAFTIPIF-----------------QC 85

Query: 148 ENGHIACSSCCNKLKNRCATCSWPIGYNRCRAVEKVLESIKLPCKQSKYGCKEMVSYGSE 207
           +NGH+ACSSCC KL N+C  C+ P+G+NRCRA+E VLESI +PC  +K GCK+ VSYG E
Sbjct: 86  DNGHLACSSCCPKLNNKCPACTSPVGHNRCRAMESVLESILIPCPNAKLGCKKNVSYGKE 145

Query: 208 SDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFSSKHTD--SAISFQFSSSFTICLNANDN 267
             HEK+C+ + C CP  DCN+ +S K L  H+   H +     +F      ++ +N +  
Sbjct: 146 LTHEKECMFSHCACPALDCNYTSSYKDLYTHYRITHMEINQINTFICDIPLSVRMNISKK 205

Query: 268 YRVLQEQGDGILFILSNSFDHLGNAVKVCCLRPPSLK-EAFSYDLRVETQDLSLVLQS-S 327
             +  E     LF +    +  G  V V C+ P S +   +SY L       +++ QS  
Sbjct: 206 ILIRTEHLTNHLFAVQCFREPYGVYVTVSCIAPSSPELSQYSYALSYTVDGHTVIYQSPE 265

Query: 328 MKNIQSSSDCCPSTRFLLIPRDL 347
           +K +   S   P   F+LIP  L
Sbjct: 266 VKRVLKLSFQTPQENFMLIPNSL 268

[Close]
BLAST of Carg18263 vs. Swiss-Prot
Match: sp|Q9FKD9|SINL6_ARATH (Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702 GN=At5g37870 PE=3 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 5.1e-39
Identity = 95/287 (33.10%), Postives = 151/287 (52.61%), Query Frame = 0 

Query: 80  EEDGTENALC-SVKSCSRNVKENGSISMFLIDPEVLDCSICCEPLSVPIFQRPQFHHDSF 139
           E +G++   C S+ S     + + + S  L D ++LDC IC + L +P+F          
Sbjct: 8   ERNGSQKRHCSSISSDDGRKRVDKTRSAMLTDLDILDCPICYQALKIPVF---------- 67

Query: 140 TIVVALRQCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAVEKVLESIKLPCKQSKYGC 199
                  QC NGH+ACSSCC KL+N+C  C+ P+G+ RCRA+E+VLES+ +PC+ +  GC
Sbjct: 68  -------QCGNGHLACSSCCPKLRNKCPACALPVGHIRCRAMERVLESVLVPCRYADLGC 127

Query: 200 KEMVSYGSESDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFSSKHTDSAISFQFS--SSF 259
            + + YG ES HEK C  +PC CP+  CN+  S K L  H+   H+  + ++ F+  S  
Sbjct: 128 TKTIYYGRESTHEKICNFSPCSCPVQGCNYTGSYKDLYEHYDLTHSTGSTAYSFNGVSYI 187

Query: 260 TICLNANDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRP--PSLKEAFSYDLRVET- 319
              +  +D   + +     +LF++    +  G  V V C+ P  P + E FSY L   T 
Sbjct: 188 AAMMFISDKILIERVYEKKLLFVVQCFEEPCGVYVSVSCIAPSAPEVGE-FSYGLLYTTW 247

Query: 320 QDLSLVLQS-SMKNIQSSSDCCPSTRFLLIPRDLFSSSGMIKLNICI 360
           + +++  QS  +K +   S   P   F+LIP  L     ++ + +CI
Sbjct: 248 EGVTMTYQSPKVKKVLKVSSQRPKDSFMLIPHSLLCGP-LLGMMLCI 275

[Close]
BLAST of Carg18263 vs. Swiss-Prot
Match: sp|Q9FKD5|SINL9_ARATH (Putative E3 ubiquitin-protein ligase SINA-like 9 OS=Arabidopsis thaliana OX=3702 GN=At5g37910 PE=3 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 5.2e-36
Identity = 90/283 (31.80%), Postives = 141/283 (49.82%), Query Frame = 0 

Query: 87  ALCSVKSCSRNVKENGSISMFLIDPEVLDCSICCEPLSVPIFQRPQFHHDSFTIVVALRQ 146
           AL S       V +    ++ L+D ++LDC ICCE L+ PIF                 Q
Sbjct: 9   ALISQGDGGERVAKRQRSAIVLLDLDILDCPICCEALTSPIF-----------------Q 68

Query: 147 CENGHIACSSCCNKLKNRCATCSWPIGYNRCRAVEKVLESIKLPCKQSKYGCKEMVSYGS 206
           C+NGH+AC SCC KL N+C  C+ P+G++R RA+E VLESI +PC   ++GC +   YG 
Sbjct: 69  CDNGHLACGSCCPKLSNKCPACTLPVGHSRSRAMESVLESILIPCPNVRFGCTKSFFYGK 128

Query: 207 ESDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFSSKHTDSAI----SFQFSSSFTICLNA 266
           ES HEK+C+ + C CP   C++  S K L  H+   H+ +       F+ ++ FT  +  
Sbjct: 129 ESAHEKECIFSQCSCPSSVCDYTGSYKDLYAHYKLTHSTNIFWNIKRFRCANFFTTSMLI 188

Query: 267 NDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRP--PSLKEAFSYDLRVETQDLSLVL 326
           +D   + +     +L  +    +  G  V V  + P  P + E FSY L       ++  
Sbjct: 189 SDKILIKRVHEKKLLLAVQCFREPCGVYVTVSFIAPSAPEVGE-FSYQLSYNVDGHTVTY 248

Query: 327 QS-SMKNIQSSSDCCPSTRFLLIPRDLFSSSGMIKLNICIWQN 363
           +S  +K +   S   P   F+LIP  L     ++++ + I +N
Sbjct: 249 ESPEVKRVCKVSIETPQENFMLIPHSLLRGD-LLEMQVFIIEN 272

[Close]
BLAST of Carg18263 vs. Swiss-Prot
Match: sp|Q9C6H3|SINL2_ARATH (E3 ubiquitin-protein ligase SINA-like 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66620 PE=2 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.0e-35
Identity = 100/299 (33.44%), Postives = 138/299 (46.15%), Query Frame = 0 

Query: 66  QRKRHRIDGIEEQVEEDGTENALCSVKSCSRNVKENGSISMFLIDPEVLDCSICCEPLSV 125
           +RKR R+    E VE  G +            V  +G+    L + ++LDC ICC  L+ 
Sbjct: 9   RRKRQRVPSSVESVENGGGD-----------AVARSGT----LFELDLLDCPICCHALTS 68

Query: 126 PIFQRPQFHHDSFTIVVALRQCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAVEKVLE 185
           PIF                 QC+NGHIACSSCC KL+N+C +C+ PIG  R R +E+V+E
Sbjct: 69  PIF-----------------QCDNGHIACSSCCTKLRNKCPSCALPIGNFRSRIMERVVE 128

Query: 186 SIKLPCKQSKYGCKEMVSYGSESDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFSSKHTD 245
           ++ + C   K+GC E  SYG E  HEK C  A C CP P+CN+    K L  HF   H D
Sbjct: 129 AVMVTCPNVKHGCTEKFSYGKELIHEKDCRFALCYCPAPNCNYSGVYKDLYSHFYVNHYD 188

Query: 246 SAISFQFSSSFTICLNANDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRP--PSLKE 305
           +       +     L  ++   VLQ  G G L  +    +  G  V V C+ P  P + E
Sbjct: 189 TWNQIGCGNFAGAWLRISEKILVLQ-YGQGPLIAVQCFKETQGMYVTVNCIAPCAPGVGE 248

Query: 306 ---AFSYDLRVETQDLSLVLQSSMKNIQSSSDCCPSTRFLLIPRDLFSSSGMIKLNICI 360
                SY + +      +     M  IQ  S   P   F+L+P         +K+ ICI
Sbjct: 249 LSFELSYKMPMGGNSTMMFKSEEMNRIQKVSFQTPEKDFMLVPYYFLGDFSTLKMEICI 274

[Close]
BLAST of Carg18263 vs. TrEMBL
Match: tr|A0A1S3CD49|A0A1S3CD49_CUCME (E3 ubiquitin-protein ligase OS=Cucumis melo OX=3656 GN=LOC103499310 PE=3 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 6.7e-145
Identity = 262/366 (71.58%), Postives = 294/366 (80.33%), Query Frame = 0 

Query: 1   MAKFSVGGEEDGEDGSSTSSPRAKRKRTSFRINAVVNFPRHNQRCEEGEEQGFDYCKEET 60
           MAKFSV GEED  DG   SSPR KRKR+SFRINA V+FPR +++ E+GEEQGF+  K   
Sbjct: 1   MAKFSVDGEEDAGDG--PSSPRGKRKRSSFRINATVDFPRRSEQREQGEEQGFNSDKXXX 60

Query: 61  ASDEEQRKRHRIDGIEEQVEEDGTENALCSVKSCSRNVKENGSISMFLIDPEVLDCSICC 120
                     R D IEE + EDG + AL S KSC++N K NGS+SMFLIDPEVLDCSICC
Sbjct: 61  XXXXXXXXXXRNDEIEEHLREDGGKRALWSGKSCTKNAKPNGSVSMFLIDPEVLDCSICC 120

Query: 121 EPLSVPIFQRPQFHHDSFTIVVALRQCENGHIACSSCCNKLKNRCATCSWPIGYNRCRAV 180
           EPLS+PIF                 QCENGHIACSSCCNKLKN+CATCSWPIGYNRCRAV
Sbjct: 121 EPLSIPIF-----------------QCENGHIACSSCCNKLKNKCATCSWPIGYNRCRAV 180

Query: 181 EKVLESIKLPCKQSKYGCKEMVSYGSESDHEKKCLHAPCKCPLPDCNFVASSKQLSLHFS 240
           EKVLESIKLPCKQSK+GCKE++ YG+ESDHEK CL+APC CP  DC F+AS+KQLSLHFS
Sbjct: 181 EKVLESIKLPCKQSKFGCKEIIGYGNESDHEKTCLYAPCLCPYSDCKFIASAKQLSLHFS 240

Query: 241 SKHTDSAISFQFSSSFTICLNANDNYRVLQEQGDGILFILSNSFDHLGNAVKVCCLRPPS 300
           +KHT+SA +F FSSSFTICL  ND Y VLQEQ DG+LFILSNS ++LG+ VK+CCL+PPS
Sbjct: 241 NKHTNSATNFHFSSSFTICLKTNDTYHVLQEQ-DGVLFILSNSSENLGSVVKICCLQPPS 300

Query: 301 LKEAFSYDLRVETQDLSLVLQSSMKNIQSSSDCCPSTRFLLIPRDLFSSSGMIKLNICIW 360
           LKE FSYDLR ETQDLSLVLQSSMKNIQSSSDCC STRFL+IPRDLFSSSGMIKLNICIW
Sbjct: 301 LKETFSYDLRAETQDLSLVLQSSMKNIQSSSDCCHSTRFLMIPRDLFSSSGMIKLNICIW 346

Query: 361 QNTGAP 367
           QN  +P
Sbjct: 361 QNGSSP 346

[Close]
BLAST of Carg18263 vs. TrEMBL
Match: tr|A0A2P4HMZ2|A0A2P4HMZ2_QUESU (E3 ubiquitin-protein ligase OS=Quercus suber OX=58331 GN=CFP56_61552 PE=3 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 8.9e-81
Identity = 179/384 (46.61%), Postives = 220/384 (57.29%), Query Frame = 0 

Query: 1   MAKFSVGGEEDGEDGSSTSSPRAKRKRTSFRINAV-VNFPRHNQRCEEGEEQGFDYCKEE 60
           MAKFSVGGEEDGE  S  SSPR KR R S + N +  N P+                   
Sbjct: 1   MAKFSVGGEEDGEGPS--SSPRPKRLRISVQTNFLDQNDPQRXXXXXXXXXXXXXXXXXX 60

Query: 61  TASDEEQRKRHRIDGIEEQVE--------------------EDGTENALCSVKSCSRNVK 120
                                                     D T     ++ + S  + 
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQGSDVTVGPRSTIGNDSHVIH 120

Query: 121 -ENGSISMFLIDPEVLDCSICCEPLSVPIFQRPQFHHDSFTIVVALRQCENGHIACSSCC 180
             +GSIS+ L DPEVLDCSIC E LS P+F                 QCENGHIACSSCC
Sbjct: 121 VGDGSISVTLTDPEVLDCSICLEHLSPPVF-----------------QCENGHIACSSCC 180

Query: 181 NKLKNRCATCSWPIGYNRCRAVEKVLESIKLPCKQSKYGCKEMVSYGSESDHEKKCLHAP 240
           NKLKN+C +C WPIGYNRCRA+EKVLE++K+PC+ SKYGCKE+VSY  +SDHEK C++AP
Sbjct: 181 NKLKNKCPSCFWPIGYNRCRAIEKVLEAVKIPCQNSKYGCKEVVSYSRKSDHEKTCIYAP 240

Query: 241 CKCPLPDCNFVASSKQLSLHFSSKHTDSAISFQFSSSFTICLNANDNYRVLQEQGDGILF 300
           C CPL  C F+ASSKQ+ +HFSSKHT+SAI F++ S+F + L   D + VLQEQ +G+LF
Sbjct: 241 CSCPLSHCKFIASSKQIYVHFSSKHTNSAIGFKYDSNFFVSLTLTDKFLVLQEQSNGVLF 300

Query: 301 ILSNSFDHLGNAVKVCCLRPPSLKEAFSYDLRVETQDLSLVLQSSMKNIQSSSDCCPSTR 360
           +L+N  DH GN V + C+ P S    FSY L V+TQ  SL L+S  KN Q   D  PST 
Sbjct: 301 VLNNCVDHRGNVVAINCVGPSSTTGGFSYGLAVKTQGSSLRLESFTKNSQHRVDSPPSTG 360

Query: 361 FLLIPRDLFSSSGMIKLNICIWQN 363
           FLLIP D F S G +KL + I +N
Sbjct: 361 FLLIPTDFFGSCGQLKLEVYINRN 365

[Close]
BLAST of Carg18263 vs. TrEMBL
Match: tr|A0A2P6RGT3|A0A2P6RGT3_ROSCH (E3 ubiquitin-protein ligase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr3g0493571 PE=3 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 8.9e-73
Identity = 136/260 (52.31%), Postives = 179/260 (68.85%), Query Frame = 0 

Query: 103 SISMFLIDPEVLDCSICCEPLSVPIFQRPQFHHDSFTIVVALRQCENGHIACSSCCNKLK 162
           SIS+ L DP+VLDC ICCE L+VP+F                 QCENGHIACSSC  K+K
Sbjct: 111 SISITLTDPDVLDCPICCESLTVPVF-----------------QCENGHIACSSCSTKIK 170

Query: 163 NRCATCSWPIGYNRCRAVEKVLESIKLPCKQSKYGCKEMVSYGSESDHEKKCLHAPCKCP 222
           N+C +CSWPIGYNRCRA+EKVLESI++ C+  KYGCKE ++  ++++HEK C+H+PC CP
Sbjct: 171 NKCPSCSWPIGYNRCRAIEKVLESIRMSCQNIKYGCKERMTCNNKNEHEKACMHSPCSCP 230

Query: 223 LPDCNFVASSKQLSLHFSSKHTDSAISFQFSSSFTICLNANDNYRVLQEQGDGILFILSN 282
              CNFV+S+K+L  HFSS H +SA  F ++SSF+I +N ND + VLQEQ DGILFIL N
Sbjct: 231 HSGCNFVSSTKKLYQHFSSNHLNSATRFLYNSSFSITINFNDKFLVLQEQNDGILFILDN 290

Query: 283 SFDHLGNAVKVCCLRPPSLKEAFSYDLRVETQDLSLVLQSSMKNIQSSSDCCPSTRFLLI 342
           + + LGN V++ C++ P     F YDL  +T+   L LQS   +  S      S+R L+I
Sbjct: 291 TTEILGNMVRLSCIQ-PRFMGGFFYDLTAKTKGCLLKLQSFTNSTPSHCHSPTSSRSLII 350

Query: 343 PRDLFSSSGMIKLNICIWQN 363
           P D FSS G +K++ICIW+N
Sbjct: 351 PGDFFSSCGQLKMDICIWRN 352

[Close]
BLAST of Carg18263 vs. TrEMBL
Match: tr|A0A2R6RKR1|A0A2R6RKR1_ACTCH (E3 ubiquitin-protein ligase OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc05900 PE=3 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 5.8e-72
Identity = 132/261 (50.57%), Postives = 171/261 (65.52%), Query Frame = 0 

Query: 101 NGSISMFLIDPEVLDCSICCEPLSVPIFQRPQFHHDSFTIVVALRQCENGHIACSSCCNK 160
           +GSIS+ L DP+VLDC IC EPLS P+F                 QCENGH+ACSSCC K
Sbjct: 96  DGSISVTLTDPDVLDCPICLEPLSSPVF-----------------QCENGHVACSSCCIK 155

Query: 161 LKNRCATCSWPIGYNRCRAVEKVLESIKLPCKQSKYGCKEMVSYGSESDHEKKCLHAPCK 220
           L+N+C +CSWPIGYNRCRA+EKV+ES+K+ C+  KYGCKE V Y  +  HEK C  APC 
Sbjct: 156 LRNKCPSCSWPIGYNRCRAIEKVIESVKISCENLKYGCKETVIYSKKHGHEKACTFAPCS 215

Query: 221 CPLPDCNFVASSKQLSLHFSSKHTDSAISFQFSSSFTICLNANDNYRVLQEQGDGILFIL 280
           CPL  C F+ SS QLSLHFSS H +S   F+++  F I L       +L+EQ +G +F+L
Sbjct: 216 CPLSACTFLGSSNQLSLHFSSTHPNSGKHFRYNCLFPINLEMRQKCLILKEQSEGKIFVL 275

Query: 281 SNSFDHLGNAVKVCCLRPPSLKEAFSYDLRVETQDLSLVLQSSMKNIQSSSDCCPSTRFL 340
           +N+F+ +GNAV + C+ P S K +FSYDL       SL LQS  KN+    +  P+ RFL
Sbjct: 276 NNAFEIMGNAVNISCIGPSSSKSSFSYDLIARVGGSSLKLQSFTKNVPRWVEHTPAERFL 335

Query: 341 LIPRDLFSSSGMIKLNICIWQ 362
           L+P + F S G +KL +CIW+
Sbjct: 336 LVPNNFFGSCGQLKLELCIWR 339

[Close]
BLAST of Carg18263 vs. TrEMBL
Match: tr|M5XIS0|M5XIS0_PRUPE (E3 ubiquitin-protein ligase OS=Prunus persica OX=3760 GN=PRUPE_3G152000 PE=3 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.4e-70
Identity = 133/266 (50.00%), Postives = 173/266 (65.04%), Query Frame = 0 

Query: 101 NGSISMFLIDPEVLDCSICCEPLSVPIFQRPQFHHDSFTIVVALRQCENGHIACSSCCNK 160
           +GS+   L DPEVLDC IC EPL++P+F                 QCENGHIACSSCC K
Sbjct: 165 DGSLVFTLADPEVLDCLICYEPLTIPVF-----------------QCENGHIACSSCCTK 224

Query: 161 LKNRCATCSWPIGYNRCRAVEKVLESIKLPCKQSKYGCKEMVSYGSESDHEKKCLHAPCK 220
            KN+C +CSWPIGYNRCRA+EK+LESI++ C+  K+GCKEMV+Y  +++HEK C+++PC 
Sbjct: 225 SKNKCPSCSWPIGYNRCRAIEKILESIRISCRNIKHGCKEMVTYNKKNEHEKACMYSPCS 284

Query: 221 CPLPDCNFVASSKQLSLHFSSKHTDSAISFQFSSSFTICLNANDNYRVLQEQGDGILFIL 280
           CPL  CNF++SSKQL LHF+  H D A  F +   F+I LN  D + VLQE+ +GILFIL
Sbjct: 285 CPLSGCNFISSSKQLYLHFTVSHVDYATRFLYGIDFSITLNIKDKFLVLQEKREGILFIL 344

Query: 281 SNSFDHLGNAVKVCCLRPPSLKEAFSYDLRVETQDLSLVLQSSMKNIQSSSDCCPSTRFL 340
            N  + LGN V + C++ P  K  F YDL  +T   SL  QS  KN         ST + 
Sbjct: 345 DNHAEKLGNLVSLRCIQ-PIFKGGFYYDLLAKTNGSSLRFQSFTKNSAGQVISPSSTGYF 404

Query: 341 LIPRDLFSSSGMIKLNICIWQNTGAP 367
           +IP D F SSG + +++CIW+N   P
Sbjct: 405 IIPSDFF-SSGKLNMDLCIWRNGQCP 411

The following BLAST results are available for this feature:
BLAST of Carg18263 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022956630.11.3e-20095.37E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita moschata][more]
XP_023534163.11.1e-19693.73E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita pepo subsp. pepo][more]
XP_022990964.19.6e-18890.19E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita maxima][more]
XP_008460511.11.0e-14471.58PREDICTED: E3 ubiquitin-protein ligase SINA-like 7 [Cucumis melo][more]
XP_022141698.13.4e-12464.50E3 ubiquitin-protein ligase SINA-like 10 [Momordica charantia][more]
BLAST of Carg18263 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G37930.13.2e-5239.86Protein with RING/U-box and TRAF-like domains[more]
AT5G37890.18.7e-4236.50Protein with RING/U-box and TRAF-like domains[more]
AT5G37870.12.8e-4033.10Protein with RING/U-box and TRAF-like domains[more]
AT5G37910.12.9e-3731.80Protein with RING/U-box and TRAF-like domains[more]
AT1G66620.11.1e-3633.44Protein with RING/U-box and TRAF-like domains[more]
BLAST of Carg18263 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q84K34|SIL10_ARATH5.8e-5139.86E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g... [more]
sp|Q9FKD7|SINL7_ARATH1.6e-4036.50E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g3... [more]
sp|Q9FKD9|SINL6_ARATH5.1e-3933.10Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702... [more]
sp|Q9FKD5|SINL9_ARATH5.2e-3631.80Putative E3 ubiquitin-protein ligase SINA-like 9 OS=Arabidopsis thaliana OX=3702... [more]
sp|Q9C6H3|SINL2_ARATH2.0e-3533.44E3 ubiquitin-protein ligase SINA-like 2 OS=Arabidopsis thaliana OX=3702 GN=At1g6... [more]
BLAST of Carg18263 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3CD49|A0A1S3CD49_CUCME6.7e-14571.58E3 ubiquitin-protein ligase OS=Cucumis melo OX=3656 GN=LOC103499310 PE=3 SV=1[more]
tr|A0A2P4HMZ2|A0A2P4HMZ2_QUESU8.9e-8146.61E3 ubiquitin-protein ligase OS=Quercus suber OX=58331 GN=CFP56_61552 PE=3 SV=1[more]
tr|A0A2P6RGT3|A0A2P6RGT3_ROSCH8.9e-7352.31E3 ubiquitin-protein ligase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr3g0493571 ... [more]
tr|A0A2R6RKR1|A0A2R6RKR1_ACTCH5.8e-7250.57E3 ubiquitin-protein ligase OS=Actinidia chinensis var. chinensis OX=1590841 GN=... [more]
tr|M5XIS0|M5XIS0_PRUPE1.4e-7050.00E3 ubiquitin-protein ligase OS=Prunus persica OX=3760 GN=PRUPE_3G152000 PE=3 SV=... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO:0008270zinc ion binding
Vocabulary: Biological Process
TermDefinition
GO:0006511ubiquitin-dependent protein catabolic process
GO:0007275multicellular organism development
Vocabulary: Cellular Component
TermDefinition
GO:0005634nucleus
Vocabulary: INTERPRO
TermDefinition
IPR008974TRAF-like
IPR013010Znf_SIAH
IPR004162SINA-like
IPR013083Znf_RING/FYVE/PHD
IPR0181217-in-absentia-prot_TRAF-dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg18263-RACarg18263-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 176..344
e-value: 1.4E-19
score: 70.7
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 181..239
e-value: 1.8E-15
score: 58.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availablePANTHERPTHR10315:SF43E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 1-RELATEDcoord: 86..362
IPR004162E3 ubiquitin-protein ligase SIN-likePANTHERPTHR10315E3 UBIQUITIN PROTEIN LIGASE SIAHcoord: 86..362
IPR013010Zinc finger, SIAH-typePROSITEPS51081ZF_SIAHcoord: 186..244
score: 14.177
IPR008974TRAF-likeSUPERFAMILYSSF49599TRAF domain-likecoord: 186..359