CSPI06G05810 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI06G05810
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	EF hand calcium-binding family protein
Location	Chr6 : 5196446 .. 5197472 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTAAGAGCCGTAGGACTTTAAACTTCTCTTCTACTTACATAAACTGTTTCTTTTCACAATTTCTCTTATAGAAAAATTTCACTTCTCTTAGTTATGTTCAATCTTACCTCTTCCTTATTCCCTACTTTCTTCCATAGGAATATTTCCTCAAAACCCTTCTTCCAAAAATCCCTAAACAACCAAAAGTTACCATCTTTCACCACTTTTAACCACTTCCAGCTCCTCTTCAACCTCCTCGACTCCGATGGTGACGGCAAGATCTCGATGAAAGAGCTCAGTCAATTTCTCTATCGTCTAGGATATAAAAAGCTGAAAGCTACAATGGAAGCTGAAGAAATGGTGAAGGAAATGGATTCAGACCGTGATGGATTCATTGAAATGGATGAATTTTTAGAGTGAGTATGACTTACATATTAGTTTAAGCACTTTTTATAAAACGTGCTTCAACTTTTGATCTAACATAGACCATTTAAATACTACTTTGATTTCATTTTAATCTTTATACTTTCAAATTTGGCTTTTTTTTTTTTTTTGACTGTACTTGTATATTTTGGAAAATTTAGAGAGACGACTGGGTTAGGCGTAACGGATGGTAAACACATTCTTAGCTTTTAATTTTGATGACGAAACGTTTTAATATAGGGTTTTGCAGAAGGGGGAGGAAGAAGAAGAAATTCTTAGGGAAGCTTTTCTGATATTTGATGGTGATAAGAATGGATTGATTTGTGCTGAGAAATTAAAGAGAACATTGAGCAAATTTGGATGGAAAAAATGTGACCTCAAACAATGTAAAAAGATGATTGAAGGAGTTGATCATGATGGCGATGGCTTTGTGAATTTTCAAGACTTTCGGCTCATGATGACACAGAAAAGATAATCATATATATAATATAGAGATTATTATCTGTAATTTTATATTTGATTTATCTCTAAGAAATGTAATGTCGTCTTTAATTAATGCTTATGATTATGACTTGTAATAATATATCTTCTTTTTCTTTTCTTTTTTTAAAAATTGTAATGGG

mRNA sequence

ATGTTCAATCTTACCTCTTCCTTATTCCCTACTTTCTTCCATAGGAATATTTCCTCAAAACCCTTCTTCCAAAAATCCCTAAACAACCAAAAGTTACCATCTTTCACCACTTTTAACCACTTCCAGCTCCTCTTCAACCTCCTCGACTCCGATGGTGACGGCAAGATCTCGATGAAAGAGCTCAGTCAATTTCTCTATCGTCTAGGATATAAAAAGCTGAAAGCTACAATGGAAGCTGAAGAAATGGTGAAGGAAATGGATTCAGACCGTGATGGATTCATTGAAATGGATGAATTTTTAGAGGTTTTGCAGAAGGGGGAGGAAGAAGAAGAAATTCTTAGGGAAGCTTTTCTGATATTTGATGGTGATAAGAATGGATTGATTTGTGCTGAGAAATTAAAGAGAACATTGAGCAAATTTGGATGGAAAAAATGTGACCTCAAACAATGTAAAAAGATGATTGAAGGAGTTGATCATGATGGCGATGGCTTTGTGAATTTTCAAGACTTTCGGCTCATGATGACACAGAAAAGATAA

Coding sequence (CDS)

ATGTTCAATCTTACCTCTTCCTTATTCCCTACTTTCTTCCATAGGAATATTTCCTCAAAACCCTTCTTCCAAAAATCCCTAAACAACCAAAAGTTACCATCTTTCACCACTTTTAACCACTTCCAGCTCCTCTTCAACCTCCTCGACTCCGATGGTGACGGCAAGATCTCGATGAAAGAGCTCAGTCAATTTCTCTATCGTCTAGGATATAAAAAGCTGAAAGCTACAATGGAAGCTGAAGAAATGGTGAAGGAAATGGATTCAGACCGTGATGGATTCATTGAAATGGATGAATTTTTAGAGGTTTTGCAGAAGGGGGAGGAAGAAGAAGAAATTCTTAGGGAAGCTTTTCTGATATTTGATGGTGATAAGAATGGATTGATTTGTGCTGAGAAATTAAAGAGAACATTGAGCAAATTTGGATGGAAAAAATGTGACCTCAAACAATGTAAAAAGATGATTGAAGGAGTTGATCATGATGGCGATGGCTTTGTGAATTTTCAAGACTTTCGGCTCATGATGACACAGAAAAGATAA

Homology

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BLAST of CSPI06G05810 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.2e-22
Identity = 59/161 (36.65%), Postives = 97/161 (60.25%), Query Frame = 1


Query: 18  SSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATM 77
           +  P+ QK+ + +     T     + +F   D +GDGKIS KEL   +  LG++  +   
Sbjct: 20  ADSPYLQKARSGK-----TEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEE-- 79

Query: 78  EAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEIL---REAFLIFDGDKNGLICAEKLK 137
           E E+ + E+D   DG+I  +EF+E+  KG ++ ++L   ++AF ++D D NG I AE+L 
Sbjct: 80  ELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELH 139

Query: 138 RTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
             L   G  +C + +C+KMI GVD DGDG ++F++F++MMT
Sbjct: 140 EVLRSLG-DECSIAECRKMIGGVDKDGDGTIDFEEFKIMMT 172

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BLAST of CSPI06G05810 vs. Swiss-Prot
Match: CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 8.7e-21
Identity = 56/132 (42.42%), Postives = 84/132 (63.64%), Query Frame = 1


Query: 44  LFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVL 103
           +F+  DS+GDGKIS+ EL      +G    +   E   +++E+D+DRDG+I +DEF  + 
Sbjct: 27  VFDQFDSNGDGKISVLELGGVFKAMGTSYTET--ELNRVLEEVDTDRDGYINLDEFSTLC 86

Query: 104 QKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDG 163
           +      EI R+AF ++D DKNGLI A +L + L++ G   C ++ C +MI  VD DGDG
Sbjct: 87  RSSSSAAEI-RDAFDLYDQDKNGLISASELHQVLNRLGMS-CSVEDCTRMIGPVDADGDG 146

Query: 164 FVNFQDFRLMMT 176
            VNF++F+ MMT
Sbjct: 147 NVNFEEFQKMMT 154

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BLAST of CSPI06G05810 vs. Swiss-Prot
Match: CALM_PYUSP (Calmodulin OS=Pyuridae sp. PE=1 SV=2)

HSP 1 Score: 100.5 bits (249), Expect = 1.9e-20
Identity = 58/140 (41.43%), Postives = 84/140 (60.00%), Query Frame = 1


Query: 41  FQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFL 100
           F+  F+L D DGDG I+ KEL   +  LG    +A  E ++M+ E+D+D DG I+  EFL
Sbjct: 13  FKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA--ELQDMINEVDADGDGTIDFPEFL 72

Query: 101 EVL---QKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGV 160
            ++    K  + EE +REAF +FD D NG I A +L+  ++  G K  D ++  +MI   
Sbjct: 73  TMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTD-EEVDEMIREA 132

Query: 161 DHDGDGFVNFQDFRLMMTQK 178
           D DGDG VN+++F  MMT K
Sbjct: 133 DIDGDGQVNYEEFVTMMTSK 149

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BLAST of CSPI06G05810 vs. Swiss-Prot
Match: CALM_PATSP (Calmodulin OS=Patinopecten sp. PE=1 SV=2)

HSP 1 Score: 98.6 bits (244), Expect = 7.4e-20
Identity = 57/140 (40.71%), Postives = 84/140 (60.00%), Query Frame = 1


Query: 41  FQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFL 100
           F+  F+L D DGDG I+ KEL   +  LG    +A  E ++M+ E+D+D DG I+  EFL
Sbjct: 13  FKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA--ELQDMINEVDADGDGTIDFPEFL 72

Query: 101 EVL---QKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGV 160
            ++    K  + EE +REAF +FD D +G I A +L+  ++  G K  D ++  +MI   
Sbjct: 73  TMMARKMKDTDSEEEIREAFRVFDKDGDGFISAAELRHVMTNLGEKLTD-EEVDEMIREA 132

Query: 161 DHDGDGFVNFQDFRLMMTQK 178
           D DGDG VN+++F  MMT K
Sbjct: 133 DIDGDGQVNYEEFVTMMTSK 149

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BLAST of CSPI06G05810 vs. Swiss-Prot
Match: CML2_ARATH (Calmodulin-like protein 2 OS=Arabidopsis thaliana GN=CML2 PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 7.4e-20
Identity = 57/135 (42.22%), Postives = 84/135 (62.22%), Query Frame = 1


Query: 44  LFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVL 103
           +F + D +GDGKI+  EL  F   +G   +    E  EM+ +MD + DG +++DEF  + 
Sbjct: 9   VFQMFDKNGDGKIAKNELKDFFKSVGI--MVPENEINEMIAKMDVNGDGAMDIDEFGSLY 68

Query: 104 QK---GEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKC-DLKQCKKMIEGVDH 163
           Q+    +EEEE +REAF +FD + +G I  E+L+  L+  G K+   L+ CKKMI  VD 
Sbjct: 69  QEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDV 128

Query: 164 DGDGFVNFQDFRLMM 175
           DGDG VNF++F+ MM
Sbjct: 129 DGDGMVNFKEFKQMM 141

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BLAST of CSPI06G05810 vs. TrEMBL
Match: A0A0A0K8Z4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G076830 PE=4 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 1.1e-94
Identity = 175/178 (98.31%), Postives = 177/178 (99.44%), Query Frame = 1


Query: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKE 60
           MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKIS KE
Sbjct: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISTKE 60

Query: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120
           LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF
Sbjct: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120

Query: 121 DGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR 179
           DGDKNGLICAEKLKRTLSKFGW+KCDLKQCKKMIEGVDHDGDG+VNFQDFRLMMTQKR
Sbjct: 121 DGDKNGLICAEKLKRTLSKFGWQKCDLKQCKKMIEGVDHDGDGYVNFQDFRLMMTQKR 178

[Close]

BLAST of CSPI06G05810 vs. TrEMBL
Match: A0A067JXS4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_14512 PE=4 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 5.6e-35
Identity = 83/164 (50.61%), Postives = 115/164 (70.12%), Query Frame = 1


Query: 15  RNISSKPFFQKSLNNQKLPSFTTF---NHFQLLFNLLDSDGDGKISMKELSQFLYRLGYK 74
           RN+SS  F     +    PSF +    N F+ +FNL+D++GDGKIS  +L + L  LG  
Sbjct: 25  RNLSSSTF-----DFSDTPSFASMEIPNQFKQVFNLIDANGDGKISSYDLIELLLCLGCD 84

Query: 75  KLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIFDGDKNGLICAE 134
           K KA  EAE MVKE+D + DGF++ DEF  +L    +EE+ L +AF+IFD DKNGLI A+
Sbjct: 85  KSKAKAEAEGMVKELDRNGDGFVDFDEFFYILNN-YDEEDYLMDAFMIFDADKNGLISAK 144

Query: 135 KLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
           +L++ LS+ G++KC LK+C+ MI+GVD DGDGFV+F++FR MMT
Sbjct: 145 ELQKVLSRLGFEKCSLKECRIMIKGVDKDGDGFVDFEEFRSMMT 182

[Close]

BLAST of CSPI06G05810 vs. TrEMBL
Match: B9RDE4_RICCO (Calmodulin, putative OS=Ricinus communis GN=RCOM_1612330 PE=4 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 7.4e-35
Identity = 81/154 (52.60%), Postives = 107/154 (69.48%), Query Frame = 1


Query: 33  PSFTTF---NHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSD 92
           PSF      N F+ +F L+D++GDGKIS  ELS+ L  LG+ K KAT EAE MV+EMD +
Sbjct: 24  PSFAAMEVHNQFKQVFKLIDANGDGKISSCELSELLLCLGFDKSKATSEAEGMVREMDCN 83

Query: 93  RDGFIEMDEFLEVLQK--------GEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFG 152
            DGF++MDEF+  L          G+ +++ L +AFLIFD DKNGLI AE+LK+ L+  G
Sbjct: 84  GDGFVDMDEFIYTLNDDGKLRGVGGDNKKDYLMDAFLIFDADKNGLISAEELKKVLTNLG 143

Query: 153 WKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
              C LK+C++MI+GVD DGDG VNF++FR MMT
Sbjct: 144 CDNCSLKKCRRMIKGVDKDGDGSVNFEEFRSMMT 177

[Close]

BLAST of CSPI06G05810 vs. TrEMBL
Match: W9QZX9_9ROSA (Putative calcium-binding protein CML25 OS=Morus notabilis GN=L484_022442 PE=4 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.4e-33
Identity = 77/149 (51.68%), Postives = 103/149 (69.13%), Query Frame = 1


Query: 39  NHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDE 98
           N F+ +F ++D +GDGKIS  ELSQ L  LGYKK  AT EAE MV+EMD + DGF+++DE
Sbjct: 55  NQFKQVFEVIDENGDGKISPSELSQVLLCLGYKKSIATKEAEGMVREMDGNGDGFVDLDE 114

Query: 99  FLEVL-----------QKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDL 158
           F++V+             G EE E L +AFL+FD DKNG I  E+L+R L K G   C L
Sbjct: 115 FMDVVINAAASDGISSSGGSEENEHLMDAFLVFDIDKNGKISPEELRRVLVKLGCDNCSL 174

Query: 159 KQCKKMIEGVDHDGDGFVNFQDFRLMMTQ 177
           ++C++MI G D +GDGFV+F++FRLMMT+
Sbjct: 175 RECRRMINGADKNGDGFVDFEEFRLMMTK 203

[Close]

BLAST of CSPI06G05810 vs. TrEMBL
Match: B9IC08_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0014s06640g PE=4 SV=2)

HSP 1 Score: 149.8 bits (377), Expect = 3.1e-33
Identity = 75/145 (51.72%), Postives = 101/145 (69.66%), Query Frame = 1


Query: 39  NHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDE 98
           N F+ +F ++D++GDGKIS  ELS+ L  LGY+K KA  EAE MV+EMD + DGFI++DE
Sbjct: 59  NQFKQVFKVIDANGDGKISCHELSEVLLCLGYEKSKAAWEAERMVREMDCNGDGFIDLDE 118

Query: 99  FLEVLQK-----GEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKM 158
           F+  +          +E+ L + FLIFD DKNGLI A +L+  L+  G KKC L+ C++M
Sbjct: 119 FINAVNDDGNFGSGNKEDYLMDVFLIFDTDKNGLISARELQTVLTSLGCKKCSLEDCRRM 178

Query: 159 IEGVDHDGDGFVNFQDFRLMMTQKR 179
           I+GVD DGDGFV+F +FR MMT  R
Sbjct: 179 IKGVDKDGDGFVDFHEFRSMMTTNR 203

[Close]

BLAST of CSPI06G05810 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 107.8 bits (268), Expect = 6.8e-24
Identity = 59/161 (36.65%), Postives = 97/161 (60.25%), Query Frame = 1


Query: 18  SSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATM 77
           +  P+ QK+ + +     T     + +F   D +GDGKIS KEL   +  LG++  +   
Sbjct: 20  ADSPYLQKARSGK-----TEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEE-- 79

Query: 78  EAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEIL---REAFLIFDGDKNGLICAEKLK 137
           E E+ + E+D   DG+I  +EF+E+  KG ++ ++L   ++AF ++D D NG I AE+L 
Sbjct: 80  ELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELH 139

Query: 138 RTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
             L   G  +C + +C+KMI GVD DGDG ++F++F++MMT
Sbjct: 140 EVLRSLG-DECSIAECRKMIGGVDKDGDGTIDFEEFKIMMT 172

[Close]

BLAST of CSPI06G05810 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 101.7 bits (252), Expect = 4.9e-22
Identity = 56/132 (42.42%), Postives = 84/132 (63.64%), Query Frame = 1


Query: 44  LFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVL 103
           +F+  DS+GDGKIS+ EL      +G    +   E   +++E+D+DRDG+I +DEF  + 
Sbjct: 27  VFDQFDSNGDGKISVLELGGVFKAMGTSYTET--ELNRVLEEVDTDRDGYINLDEFSTLC 86

Query: 104 QKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDG 163
           +      EI R+AF ++D DKNGLI A +L + L++ G   C ++ C +MI  VD DGDG
Sbjct: 87  RSSSSAAEI-RDAFDLYDQDKNGLISASELHQVLNRLGMS-CSVEDCTRMIGPVDADGDG 146

Query: 164 FVNFQDFRLMMT 176
            VNF++F+ MMT
Sbjct: 147 NVNFEEFQKMMT 154

[Close]

BLAST of CSPI06G05810 vs. TAIR10
Match: AT4G12860.1 (AT4G12860.1 EF hand calcium-binding protein family)

HSP 1 Score: 98.6 bits (244), Expect = 4.1e-21
Identity = 57/135 (42.22%), Postives = 84/135 (62.22%), Query Frame = 1


Query: 44  LFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVL 103
           +F + D +GDGKI+  EL  F   +G   +    E  EM+ +MD + DG +++DEF  + 
Sbjct: 9   VFQMFDKNGDGKIAKNELKDFFKSVGI--MVPENEINEMIAKMDVNGDGAMDIDEFGSLY 68

Query: 104 QK---GEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKC-DLKQCKKMIEGVDH 163
           Q+    +EEEE +REAF +FD + +G I  E+L+  L+  G K+   L+ CKKMI  VD 
Sbjct: 69  QEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDV 128

Query: 164 DGDGFVNFQDFRLMM 175
           DGDG VNF++F+ MM
Sbjct: 129 DGDGMVNFKEFKQMM 141

[Close]

BLAST of CSPI06G05810 vs. TAIR10
Match: AT1G05990.1 (AT1G05990.1 EF hand calcium-binding protein family)

HSP 1 Score: 97.4 bits (241), Expect = 9.2e-21
Identity = 57/136 (41.91%), Postives = 87/136 (63.97%), Query Frame = 1


Query: 44  LFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLE-- 103
           +F + D +GDG I+ KELS+ L  LG        E  +M++++D + DG +++DEF E  
Sbjct: 9   VFQMFDKNGDGTITGKELSETLRSLGI--YIPDKELTQMIEKIDVNGDGCVDIDEFGELY 68

Query: 104 --VLQKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCD-LKQCKKMIEGVD 163
             ++ + +EEEE ++EAF +FD + +G I  ++LK  LS  G K+   L  CKKMI+ VD
Sbjct: 69  KTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVD 128

Query: 164 HDGDGFVNFQDFRLMM 175
            DGDG VN+++FR MM
Sbjct: 129 VDGDGRVNYKEFRQMM 142

[Close]

BLAST of CSPI06G05810 vs. TAIR10
Match: AT3G10190.1 (AT3G10190.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 97.4 bits (241), Expect = 9.2e-21
Identity = 59/150 (39.33%), Postives = 81/150 (54.00%), Query Frame = 1


Query: 31  KLPSFTTFNHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSDR 90
           ++PS  ++      F L+D D DG +S  +L   L RLG   L    E   M+KE+D D 
Sbjct: 61  EVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEE-EINVMLKEVDCDG 120

Query: 91  DGFIEMDEFLE--VLQKGEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFGWKKCDLK 150
           DG I ++E     V      +   L+E F  FD D++GLI A++L R  S  G ++C L 
Sbjct: 121 DGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCTLD 180

Query: 151 QCKKMIEGVDHDGDGFVNFQDFRLMMTQKR 179
            CK+MI  VD DGDGFV F +F  MM  +R
Sbjct: 181 DCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of CSPI06G05810 vs. NCBI nr
Match: gi|449470826|ref|XP_004153117.1| (PREDICTED: calmodulin-beta-like [Cucumis sativus])

HSP 1 Score: 354.0 bits (907), Expect = 1.6e-94
Identity = 175/178 (98.31%), Postives = 177/178 (99.44%), Query Frame = 1


Query: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKE 60
           MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKIS KE
Sbjct: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISTKE 60

Query: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120
           LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF
Sbjct: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120

Query: 121 DGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR 179
           DGDKNGLICAEKLKRTLSKFGW+KCDLKQCKKMIEGVDHDGDG+VNFQDFRLMMTQKR
Sbjct: 121 DGDKNGLICAEKLKRTLSKFGWQKCDLKQCKKMIEGVDHDGDGYVNFQDFRLMMTQKR 178

[Close]

BLAST of CSPI06G05810 vs. NCBI nr
Match: gi|659120319|ref|XP_008460131.1| (PREDICTED: calmodulin-beta-like [Cucumis melo])

HSP 1 Score: 339.3 bits (869), Expect = 4.0e-90
Identity = 168/178 (94.38%), Postives = 171/178 (96.07%), Query Frame = 1


Query: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISMKE 60
           M NLTSSLF TFFHRNI+SKPFFQKSLNNQKLPSFTTFNHFQLLFN+LDS GDGKIS KE
Sbjct: 1   MLNLTSSLFSTFFHRNITSKPFFQKSLNNQKLPSFTTFNHFQLLFNILDSGGDGKISTKE 60

Query: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120
           LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF
Sbjct: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120

Query: 121 DGDKNGLICAEKLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR 179
           DGDKNGLICAE+LKRT  KFGW KCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR
Sbjct: 121 DGDKNGLICAEELKRTFRKFGWTKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQKR 178

[Close]

BLAST of CSPI06G05810 vs. NCBI nr
Match: gi|802699492|ref|XP_012083557.1| (PREDICTED: probable calcium-binding protein CML27 [Jatropha curcas])

HSP 1 Score: 155.6 bits (392), Expect = 8.1e-35
Identity = 83/164 (50.61%), Postives = 115/164 (70.12%), Query Frame = 1


Query: 15  RNISSKPFFQKSLNNQKLPSFTTF---NHFQLLFNLLDSDGDGKISMKELSQFLYRLGYK 74
           RN+SS  F     +    PSF +    N F+ +FNL+D++GDGKIS  +L + L  LG  
Sbjct: 25  RNLSSSTF-----DFSDTPSFASMEIPNQFKQVFNLIDANGDGKISSYDLIELLLCLGCD 84

Query: 75  KLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIFDGDKNGLICAE 134
           K KA  EAE MVKE+D + DGF++ DEF  +L    +EE+ L +AF+IFD DKNGLI A+
Sbjct: 85  KSKAKAEAEGMVKELDRNGDGFVDFDEFFYILNN-YDEEDYLMDAFMIFDADKNGLISAK 144

Query: 135 KLKRTLSKFGWKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
           +L++ LS+ G++KC LK+C+ MI+GVD DGDGFV+F++FR MMT
Sbjct: 145 ELQKVLSRLGFEKCSLKECRIMIKGVDKDGDGFVDFEEFRSMMT 182

[Close]

BLAST of CSPI06G05810 vs. NCBI nr
Match: gi|223548913|gb|EEF50402.1| (calmodulin, putative [Ricinus communis])

HSP 1 Score: 155.2 bits (391), Expect = 1.1e-34
Identity = 81/154 (52.60%), Postives = 107/154 (69.48%), Query Frame = 1


Query: 33  PSFTTF---NHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSD 92
           PSF      N F+ +F L+D++GDGKIS  ELS+ L  LG+ K KAT EAE MV+EMD +
Sbjct: 24  PSFAAMEVHNQFKQVFKLIDANGDGKISSCELSELLLCLGFDKSKATSEAEGMVREMDCN 83

Query: 93  RDGFIEMDEFLEVLQK--------GEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFG 152
            DGF++MDEF+  L          G+ +++ L +AFLIFD DKNGLI AE+LK+ L+  G
Sbjct: 84  GDGFVDMDEFIYTLNDDGKLRGVGGDNKKDYLMDAFLIFDADKNGLISAEELKKVLTNLG 143

Query: 153 WKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
              C LK+C++MI+GVD DGDG VNF++FR MMT
Sbjct: 144 CDNCSLKKCRRMIKGVDKDGDGSVNFEEFRSMMT 177

[Close]

BLAST of CSPI06G05810 vs. NCBI nr
Match: gi|1000981875|ref|XP_002511733.2| (PREDICTED: probable calcium-binding protein CML18 [Ricinus communis])

HSP 1 Score: 155.2 bits (391), Expect = 1.1e-34
Identity = 81/154 (52.60%), Postives = 107/154 (69.48%), Query Frame = 1


Query: 33  PSFTTF---NHFQLLFNLLDSDGDGKISMKELSQFLYRLGYKKLKATMEAEEMVKEMDSD 92
           PSF      N F+ +F L+D++GDGKIS  ELS+ L  LG+ K KAT EAE MV+EMD +
Sbjct: 45  PSFAAMEVHNQFKQVFKLIDANGDGKISSCELSELLLCLGFDKSKATSEAEGMVREMDCN 104

Query: 93  RDGFIEMDEFLEVLQK--------GEEEEEILREAFLIFDGDKNGLICAEKLKRTLSKFG 152
            DGF++MDEF+  L          G+ +++ L +AFLIFD DKNGLI AE+LK+ L+  G
Sbjct: 105 GDGFVDMDEFIYTLNDDGKLRGVGGDNKKDYLMDAFLIFDADKNGLISAEELKKVLTNLG 164

Query: 153 WKKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMT 176
              C LK+C++MI+GVD DGDG VNF++FR MMT
Sbjct: 165 CDNCSLKKCRRMIKGVDKDGDGSVNFEEFRSMMT 198

The following BLAST results are available for this feature:

BLAST of CSPI06G05810 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML25_ARATH	1.2e-22	36.65	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=...	[more]
CML27_ARATH	8.7e-21	42.42	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=...	[more]
CALM_PYUSP	1.9e-20	41.43	Calmodulin OS=Pyuridae sp. PE=1 SV=2	[more]
CALM_PATSP	7.4e-20	40.71	Calmodulin OS=Patinopecten sp. PE=1 SV=2	[more]
CML2_ARATH	7.4e-20	42.22	Calmodulin-like protein 2 OS=Arabidopsis thaliana GN=CML2 PE=2 SV=1	[more]

BLAST of CSPI06G05810 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K8Z4_CUCSA	1.1e-94	98.31	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G076830 PE=4 SV=1	[more]
A0A067JXS4_JATCU	5.6e-35	50.61	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_14512 PE=4 SV=1	[more]
B9RDE4_RICCO	7.4e-35	52.60	Calmodulin, putative OS=Ricinus communis GN=RCOM_1612330 PE=4 SV=1	[more]
W9QZX9_9ROSA	1.4e-33	51.68	Putative calcium-binding protein CML25 OS=Morus notabilis GN=L484_022442 PE=4 SV...	[more]
B9IC08_POPTR	3.1e-33	51.72	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0014s06640g PE=4 SV=2	[more]

BLAST of CSPI06G05810 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G24620.1	6.8e-24	36.65	EF hand calcium-binding protein family	[more]
AT1G18210.1	4.9e-22	42.42	Calcium-binding EF-hand family protein	[more]
AT4G12860.1	4.1e-21	42.22	EF hand calcium-binding protein family	[more]
AT1G05990.1	9.2e-21	41.91	EF hand calcium-binding protein family	[more]
AT3G10190.1	9.2e-21	39.33	Calcium-binding EF-hand family protein	[more]

BLAST of CSPI06G05810 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449470826\|ref\|XP_004153117.1\|	1.6e-94	98.31	PREDICTED: calmodulin-beta-like [Cucumis sativus]	[more]
gi\|659120319\|ref\|XP_008460131.1\|	4.0e-90	94.38	PREDICTED: calmodulin-beta-like [Cucumis melo]	[more]
gi\|802699492\|ref\|XP_012083557.1\|	8.1e-35	50.61	PREDICTED: probable calcium-binding protein CML27 [Jatropha curcas]	[more]
gi\|223548913\|gb\|EEF50402.1\|	1.1e-34	52.60	calmodulin, putative [Ricinus communis]	[more]
gi\|1000981875\|ref\|XP_002511733.2\|	1.1e-34	52.60	PREDICTED: probable calcium-binding protein CML18 [Ricinus communis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G05810.1	CSPI06G05810.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 42..103 score: 4.3E-12coord: 113..174 score: 2.
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 40..68 score: 0.0026coord: 112..140 score: 0.91coord: 149..177 score: 0.0015coord: 78..106 score: 0
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 145..178 score: 11.584coord: 110..143 score: 10.245coord: 36..71 score: 13.37coord: 74..109 score: 12
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 91..175 score: 1.6E-20coord: 40..90 score: 4.9
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 37..175 score: 2.0
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 49..61 score: -coord: 158..170 score: -coord: 87..99 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 41..176 score: 6.6
None	No IPR available	PANTHER	PTHR10891:SF573	CALCIUM-BINDING PROTEIN CML24-RELATED	coord: 41..176 score: 6.6

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI06G05810	Cla006954	Watermelon (97103) v1	cpiwmB556
CSPI06G05810	Csa6G076830	Cucumber (Chinese Long) v2	cpicuB314
CSPI06G05810	MELO3C022506	Melon (DHL92) v3.5.1	cpimeB433
CSPI06G05810	ClCG06G000330	Watermelon (Charleston Gray)	cpiwcgB503
CSPI06G05810	Lsi09G019020	Bottle gourd (USVL1VR-Ls)	cpilsiB404
CSPI06G05810	MELO3C022506.2	Melon (DHL92) v3.6.1	cpimedB431
CSPI06G05810	CsaV3_6G006120	Cucumber (Chinese Long) v3	cpicucB364
CSPI06G05810	Cla97C06G109590	Watermelon (97103) v2	cpiwmbB495
CSPI06G05810	Cucsa.362850	Cucumber (Gy14) v1	cgycpiB540
CSPI06G05810	CsGy6G005790	Cucumber (Gy14) v2	cgybcpiB288

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI06G05810	Wild cucumber (PI 183967)	cpicpiB126
CSPI06G05810	Wild cucumber (PI 183967)	cpicpiB139
CSPI06G05810	Cucumber (Gy14) v1	cgycpiB391
CSPI06G05810	Cucumber (Gy14) v1	cgycpiB393
CSPI06G05810	Cucurbita maxima (Rimu)	cmacpiB273
CSPI06G05810	Cucurbita maxima (Rimu)	cmacpiB350
CSPI06G05810	Cucurbita maxima (Rimu)	cmacpiB373
CSPI06G05810	Cucurbita maxima (Rimu)	cmacpiB856
CSPI06G05810	Cucurbita maxima (Rimu)	cmacpiB915
CSPI06G05810	Cucurbita moschata (Rifu)	cmocpiB263
CSPI06G05810	Cucurbita moschata (Rifu)	cmocpiB362
CSPI06G05810	Cucurbita moschata (Rifu)	cmocpiB343
CSPI06G05810	Cucurbita moschata (Rifu)	cmocpiB839
CSPI06G05810	Cucurbita moschata (Rifu)	cmocpiB893
CSPI06G05810	Cucumber (Chinese Long) v2	cpicuB283
CSPI06G05810	Melon (DHL92) v3.5.1	cpimeB496
CSPI06G05810	Watermelon (Charleston Gray)	cpiwcgB494
CSPI06G05810	Watermelon (97103) v1	cpiwmB508
CSPI06G05810	Watermelon (97103) v1	cpiwmB517
CSPI06G05810	Cucurbita pepo (Zucchini)	cpecpiB145
CSPI06G05810	Cucurbita pepo (Zucchini)	cpecpiB324
CSPI06G05810	Cucurbita pepo (Zucchini)	cpecpiB633
CSPI06G05810	Cucurbita pepo (Zucchini)	cpecpiB879
CSPI06G05810	Bottle gourd (USVL1VR-Ls)	cpilsiB470
CSPI06G05810	Bottle gourd (USVL1VR-Ls)	cpilsiB469
CSPI06G05810	Melon (DHL92) v3.6.1	cpimedB483
CSPI06G05810	Melon (DHL92) v3.6.1	cpimedB488
CSPI06G05810	Cucumber (Gy14) v2	cgybcpiB141
CSPI06G05810	Silver-seed gourd	carcpiB0263
CSPI06G05810	Silver-seed gourd	carcpiB0653
CSPI06G05810	Silver-seed gourd	carcpiB1118
CSPI06G05810	Cucumber (Chinese Long) v3	cpicucB335
CSPI06G05810	Watermelon (97103) v2	cpiwmbB485
CSPI06G05810	Watermelon (97103) v2	cpiwmbB491
CSPI06G05810	Wax gourd	cpiwgoB559
CSPI06G05810	Wax gourd	cpiwgoB544
CSPI06G05810	Wax gourd	cpiwgoB548