CSPI03G17490 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G17490
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Heavy metal transport/detoxification domain protein
Location	Chr3 : 13124854 .. 13126259 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAAAAAATCCTTCACATTTCTTGAAGGTTGAGGTTAGTTGTTCGTTACCAAGAACATTTGATCATACTCCCTTTAATAGCCGTTTTGTTTTTTGTTGTTTCCAAAAACTTGATTTAAAAATTAAAGAATGGCTTGAAGTTCAAATATGTTTTTAAATAAGATGAAGATGAAGGCAAAACAAAATGGTTATTAGTGGATACCTTTTTTTTTTTCCATTTGTAAATATTGGTTTCTTTAATTTGCTTGTTCACACATTGTTTTAACAGACGCGTGTTCTTAAAGTACACGTTGATTGTCAAGGTTGCCTCCAGAGAGTAAGAAAACTTCTCAACAGGATTGAAGGTATTTATTATTATTTTGTTGCATGTAAAAACTAATCTATAGGTTCTTTTTTTAATTTTTATTTTATACTAAGAAATATGTACACTTTAGTTTGAATAACAGGTTTGTTTGTGACTCATGTGTGAAACACATATGAAAACTACTATGCCAAGAGTTTTATAGATCATATCTTTTGTTTTAACTTAAAAAAAAATGCATCTTTTTGATCCTATGTTTCACTTATTTTCTTCTACATGTTTATTTTTTAAAAGATAAATAAACTGTATTCTTAGTTCATGACTGTTTAGTAGATTAACATAAAAATTTATTTTCCGTAAAAAGAAAAGAAAAAAAAAAAAAAAACTCCTATTCTGTAAAATGAGCTTCTTGTGTTTCCAAAGAAAAAAATGAAGAAAAAAGGAAAAAGAAAACATCTTTTTCTTTAAAATGAGCCTTTTGTGTTTTGAAACAAATGTTTTTAAAATAGTGATTTCAAATATAACATCTTCCATTGGCTAACTTTTATTATTGGAATTAAAATATACAGCCTTATTCTCATTTGATCTTATGGAATTTAGGAGTTTATAAGATAGACATAAATTGTGAACAACAAAAGGTCACAGTGACGGGCAATGTGGATTCAACCATTTTGATAAAGAAGCTTAAAAAATTAGGTAAGCATGCTGAACTTTGGCCTTCAACTTCCAAACATGGTGAAGGTGAAGAATCAAACCTAAGAAAATACATAGACAACCAAATGAAAGATGCAACAGACCCATACTATACCTTCCAAAATCAGCACATGCTTCCTATACTTAATAGAGAATTCAACAATCGGTCGCTATTTGAACGTTACCTCGATCAAGAAAGTGGCATGTCCAACAGTTTCCGGTATCATCCAGTCACTACAACTGCAGCACAGAAGGCAAGAGCTGTCTATGATAATGAAAAGCTGGGAAACCAGATGATATCCATGGCTCATAATGTAGGCATTCAAGATTTTCAGTTTGACGGGGTTCCAGATAATACATGTGTTCGAGACTATCGATTTGGCATCTTTCCAGATTTCCCTTATGCTTGA

mRNA sequence

ATGTCAAAAAATCCTTCACATTTCTTGAAGGTTGAGACGCGTGTTCTTAAAGTACACGTTGATTGTCAAGGTTGCCTCCAGAGAGTAAGAAAACTTCTCAACAGGATTGAAGGAGTTTATAAGATAGACATAAATTGTGAACAACAAAAGGTCACAGTGACGGGCAATGTGGATTCAACCATTTTGATAAAGAAGCTTAAAAAATTAGGTAAGCATGCTGAACTTTGGCCTTCAACTTCCAAACATGGTGAAGGTGAAGAATCAAACCTAAGAAAATACATAGACAACCAAATGAAAGATGCAACAGACCCATACTATACCTTCCAAAATCAGCACATGCTTCCTATACTTAATAGAGAATTCAACAATCGGTCGCTATTTGAACGTTACCTCGATCAAGAAAGTGGCATGTCCAACAGTTTCCGGTATCATCCAGTCACTACAACTGCAGCACAGAAGGCAAGAGCTGTCTATGATAATGAAAAGCTGGGAAACCAGATGATATCCATGGCTCATAATGTAGGCATTCAAGATTTTCAGTTTGACGGGGTTCCAGATAATACATGTGTTCGAGACTATCGATTTGGCATCTTTCCAGATTTCCCTTATGCTTGA

Coding sequence (CDS)

ATGTCAAAAAATCCTTCACATTTCTTGAAGGTTGAGACGCGTGTTCTTAAAGTACACGTTGATTGTCAAGGTTGCCTCCAGAGAGTAAGAAAACTTCTCAACAGGATTGAAGGAGTTTATAAGATAGACATAAATTGTGAACAACAAAAGGTCACAGTGACGGGCAATGTGGATTCAACCATTTTGATAAAGAAGCTTAAAAAATTAGGTAAGCATGCTGAACTTTGGCCTTCAACTTCCAAACATGGTGAAGGTGAAGAATCAAACCTAAGAAAATACATAGACAACCAAATGAAAGATGCAACAGACCCATACTATACCTTCCAAAATCAGCACATGCTTCCTATACTTAATAGAGAATTCAACAATCGGTCGCTATTTGAACGTTACCTCGATCAAGAAAGTGGCATGTCCAACAGTTTCCGGTATCATCCAGTCACTACAACTGCAGCACAGAAGGCAAGAGCTGTCTATGATAATGAAAAGCTGGGAAACCAGATGATATCCATGGCTCATAATGTAGGCATTCAAGATTTTCAGTTTGACGGGGTTCCAGATAATACATGTGTTCGAGACTATCGATTTGGCATCTTTCCAGATTTCCCTTATGCTTGA

Homology

[Close]

BLAST of CSPI03G17490 vs. Swiss-Prot
Match: HIP21_ARATH (Heavy metal-associated isoprenylated plant protein 21 OS=Arabidopsis thaliana GN=HIPP21 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.4e-11
Identity = 29/72 (40.28%), Postives = 50/72 (69.44%), Query Frame = 1


Query: 11 VETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDSTILIKKLKKLG 70
          ++T  +KV +DC GC +RVR ++ R++GV  +++N +Q ++TV G+VD   ++K++K  G
Sbjct: 25 LQTVDIKVKMDCDGCERRVRNVVRRMKGVKSVEVNRKQSRITVNGHVDPNKVLKRVKSTG 84

Query: 71 KHAELWPSTSKH 83
          K AE WP   +H
Sbjct: 85 KKAEFWPYIPQH 96

[Close]

BLAST of CSPI03G17490 vs. Swiss-Prot
Match: CCH_ARATH (Copper transport protein CCH OS=Arabidopsis thaliana GN=CCH PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 7.9e-10
Identity = 29/66 (43.94%), Postives = 43/66 (65.15%), Query Frame = 1


Query: 12 ETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDSTILIKKLKKLGK 71
          +T VLKV + CQGC+  V ++L ++EGV   DI+ ++QKVTV GNV+   + + + K GK
Sbjct: 3  QTVVLKVGMSCQGCVGAVNRVLGKMEGVESFDIDIKEQKVTVKGNVEPEAVFQTVSKTGK 62

Query: 72 HAELWP 78
              WP
Sbjct: 63 KTSYWP 68

[Close]

BLAST of CSPI03G17490 vs. Swiss-Prot
Match: HIP20_ARATH (Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana GN=HIPP20 PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 8.7e-09
Identity = 25/67 (37.31%), Postives = 48/67 (71.64%), Query Frame = 1


Query: 11 VETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDSTILIKKLKKLG 70
          ++T  +KV +DC GC +RV+  ++ ++GV  +++N +  KVTV+G V+   ++K++++ G
Sbjct: 27 MQTVNIKVKMDCDGCERRVKNAVSSMKGVKSVEVNRKIHKVTVSGYVEPKKVLKRIERTG 86

Query: 71 KHAELWP 78
          K AE+WP
Sbjct: 87 KKAEIWP 93

[Close]

BLAST of CSPI03G17490 vs. Swiss-Prot
Match: HIP27_ARATH (Heavy metal-associated isoprenylated plant protein 27 OS=Arabidopsis thaliana GN=HIPP27 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.5e-08
Identity = 32/76 (42.11%), Postives = 49/76 (64.47%), Query Frame = 1


Query: 3  KNPSHFLKVETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVD-STI 62
          K    F KVE   +KV +DC+GC +RVRK +  ++GV K+ ++ +Q K+TV G V  S +
Sbjct: 13 KKLKQFQKVE---IKVKMDCEGCERRVRKSVEGMKGVSKVTVDPKQSKLTVEGFVQPSKV 72

Query: 63 LIKKLKKLGKHAELWP 78
          + + + + GK AELWP
Sbjct: 73 VHRVMHRTGKKAELWP 85

[Close]

BLAST of CSPI03G17490 vs. Swiss-Prot
Match: ATOX1_ARATH (Copper transport protein ATX1 OS=Arabidopsis thaliana GN=ATX1 PE=1 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 3.3e-08
Identity = 25/65 (38.46%), Postives = 43/65 (66.15%), Query Frame = 1


Query: 12 ETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDSTILIKKLKKLGK 71
          +T VL+V + C+GC+  V+++L ++EGV   D++ ++QKVTV GNV    +++ + K GK
Sbjct: 3  QTVVLRVAMTCEGCVGAVKRVLGKMEGVESFDVDIKEQKVTVKGNVQPDAVLQTVTKTGK 62

Query: 72 HAELW 77
              W
Sbjct: 63 KTAFW 67

[Close]

BLAST of CSPI03G17490 vs. TrEMBL
Match: A0A0A0L6R9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G185130 PE=4 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 9.8e-116
Identity = 204/204 (100.00%), Postives = 204/204 (100.00%), Query Frame = 1


Query: 1   MSKNPSHFLKVETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDST 60
           MSKNPSHFLKVETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDST
Sbjct: 1   MSKNPSHFLKVETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDST 60

Query: 61  ILIKKLKKLGKHAELWPSTSKHGEGEESNLRKYIDNQMKDATDPYYTFQNQHMLPILNRE 120
           ILIKKLKKLGKHAELWPSTSKHGEGEESNLRKYIDNQMKDATDPYYTFQNQHMLPILNRE
Sbjct: 61  ILIKKLKKLGKHAELWPSTSKHGEGEESNLRKYIDNQMKDATDPYYTFQNQHMLPILNRE 120

Query: 121 FNNRSLFERYLDQESGMSNSFRYHPVTTTAAQKARAVYDNEKLGNQMISMAHNVGIQDFQ 180
           FNNRSLFERYLDQESGMSNSFRYHPVTTTAAQKARAVYDNEKLGNQMISMAHNVGIQDFQ
Sbjct: 121 FNNRSLFERYLDQESGMSNSFRYHPVTTTAAQKARAVYDNEKLGNQMISMAHNVGIQDFQ 180

Query: 181 FDGVPDNTCVRDYRFGIFPDFPYA 205
           FDGVPDNTCVRDYRFGIFPDFPYA
Sbjct: 181 FDGVPDNTCVRDYRFGIFPDFPYA 204

[Close]

BLAST of CSPI03G17490 vs. TrEMBL
Match: A0A067JFP2_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02499 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 4.8e-22
Identity = 71/188 (37.77%), Postives = 106/188 (56.38%), Query Frame = 1


Query: 1   MSKNPSHFLKVETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDST 60
           M +N    LK+ET VLKVH++C+GC ++VRKLL +IEGVY +DI+ EQQ V V+G VDS+
Sbjct: 1   MLRNGHGILKIETHVLKVHINCEGCKEKVRKLLKKIEGVYSVDIDTEQQMVKVSGCVDSS 60

Query: 61  ILIKKLKKLGKHAELWPSTSKHGEGEES---NLRKYIDNQMKDATDPYYTFQNQHMLPIL 120
           ILIKKL K GK AE+W   SK+   EE    N ++++ N   +A+  +Y F    +    
Sbjct: 61  ILIKKLIKSGKKAEVWSPVSKYKLNEEQANINPKQFLAND-SNASKNHYMFPKSFI---- 120

Query: 121 NREFNNRSLFERYLDQESGMSNSFRYHPVTTTAAQKARAVYDNEKLGNQMI--SMAHNVG 180
             E  ++  FE++  Q   M     + PV          +YD+  +G+  +   MA  + 
Sbjct: 121 -NEIEDKGSFEKFPSQNIKMKAVDNHDPVVAKRMGN-NYMYDDIFIGDSRLEDDMAPLID 180

Query: 181 IQDFQFDG 184
             D+Q +G
Sbjct: 181 HGDYQENG 181

[Close]

BLAST of CSPI03G17490 vs. TrEMBL
Match: D7TST6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0006g01780 PE=4 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.8e-21
Identity = 63/127 (49.61%), Postives = 81/127 (63.78%), Query Frame = 1


Query: 12  ETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDSTILIKKLKKLGK 71
           +T VLKVHV+C GC Q+V+KLL+RIEGVY + I+ EQQKVTVTGNVD+  LI KL + GK
Sbjct: 7   QTCVLKVHVNCNGCKQKVKKLLSRIEGVYSVSIDAEQQKVTVTGNVDAATLINKLVRRGK 66

Query: 72  HAELWPSTSKHGEGEE-SNLRKYIDNQMKDATDPYYTFQNQHMLP-ILNREFNNRSLFER 131
           HAELWP ++   + ++ SN  K  D+ +      Y    NQHMLP     E  +R     
Sbjct: 67  HAELWPPSNHQNQNQQHSNFMKDDDHSIHPM--HYRINDNQHMLPSFYAMEDQDRWARGM 126

Query: 132 YLDQESG 137
           YL+Q  G
Sbjct: 127 YLNQSMG 131

[Close]

BLAST of CSPI03G17490 vs. TrEMBL
Match: M5XDU3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007015mg PE=4 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 5.3e-21
Identity = 70/175 (40.00%), Postives = 101/175 (57.71%), Query Frame = 1


Query: 9   LKVETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDSTILIKKLKK 68
           LK++T VLKV++ C GC Q+V+KLL RIEGVY+++I+ +QQKVTV+GNVDS  LIKKL +
Sbjct: 10  LKIQTCVLKVNIHCDGCKQKVKKLLQRIEGVYQVNIDADQQKVTVSGNVDSATLIKKLTR 69

Query: 69  LGKHAELWPSTSKHGEGEESNLRKYIDN---QMKDATDPYYTFQNQHMLPILNREFNNRS 128
            GKHAELW   S   + +++N  K   N   Q +        F+NQ   P  + E ++  
Sbjct: 70  AGKHAELWSQKSNQSQKQKNNCIKDDKNNKGQRQGIIKNLEAFKNQQKFPSFSSEEDD-- 129

Query: 129 LFERYLDQESGMSNSFRY--HPVTTTAAQKARAV-YDNEKLGNQMI--SMAHNVG 176
             E + D E    + FR     V      + +A+  +N K G  +I  + A+NVG
Sbjct: 130 --EYFDDDEEDEEDEFRLIREKVNQLGMLRQQAIDANNGKKGAGVINAAAANNVG 180

[Close]

BLAST of CSPI03G17490 vs. TrEMBL
Match: F6HFJ4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0011g04710 PE=4 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.6e-20
Identity = 70/194 (36.08%), Postives = 104/194 (53.61%), Query Frame = 1


Query: 9   LKVETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDSTILIKKLKK 68
           LK++T VLKV++ C GC Q+V+KLL RIEGVY ++I+ EQQ+VTV+G+VDS  LIKKL K
Sbjct: 10  LKIQTCVLKVNIHCDGCKQKVKKLLQRIEGVYTVNIDAEQQRVTVSGSVDSGTLIKKLVK 69

Query: 69  LGKHAELWPSTSKHGEGEESNLRKYIDN---QMKDATDPYYTFQNQHMLPILNREFNNRS 128
            GKHAELW   S   + +++N  K   N   Q +        F+ Q   P+ + E +   
Sbjct: 70  AGKHAELWSQKSNQNQKQKTNCIKDDKNNKGQKQGLIKGLEAFKTQQKFPVFSSEEDEDD 129

Query: 129 LFE----------RYLDQESGMSNSFRYHPVTTTAAQK---ARAVYDNEKLGNQMISMAH 187
             +          R+L +++   +  R   +  + A+K   A A  +N K+ N       
Sbjct: 130 FDDDEEDYEEEELRFLQEKANQLSLLRQQALDASNAKKGFGAIAASNNGKINN------- 189

[Close]

BLAST of CSPI03G17490 vs. TAIR10
Match: AT3G06130.1 (AT3G06130.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 92.0 bits (227), Expect = 4.4e-19
Identity = 44/90 (48.89%), Postives = 64/90 (71.11%), Query Frame = 1


Query: 1  MSKNPSHFLKVETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDST 60
          MSK    F+K++T VLKV++ C GC Q+V+K+L +IEGV+   I+ EQ KVTV+G+VD +
Sbjct: 1  MSKE--EFMKIQTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDSEQGKVTVSGSVDPS 60

Query: 61 ILIKKLKKLGKHAELWPSTSKHGEGEESNL 91
          +LIKKL K GKHAE+W +   +    +S +
Sbjct: 61 VLIKKLAKSGKHAEIWGAPKGNNNPNQSQM 88

[Close]

BLAST of CSPI03G17490 vs. TAIR10
Match: AT5G19090.1 (AT5G19090.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 91.3 bits (225), Expect = 7.6e-19
Identity = 49/99 (49.49%), Postives = 66/99 (66.67%), Query Frame = 1


Query: 1   MSKNPSHFLKVETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDST 60
           MSK    F+K++T VLKV++ C GC Q+V+K+L +IEGV+   I+ E  KVTV+GNVD +
Sbjct: 1   MSKE--EFMKIQTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPS 60

Query: 61  ILIKKLKKLGKHAELWPSTSKHGEGEESNLRKYIDNQMK 100
           +LIKKL K GKHAE+W    K G     N +  + NQ K
Sbjct: 61  VLIKKLLKSGKHAEIW-GAPKGGSNNNQN-QPNLANQFK 95

[Close]

BLAST of CSPI03G17490 vs. TAIR10
Match: AT5G27690.1 (AT5G27690.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 90.5 bits (223), Expect = 1.3e-18
Identity = 39/84 (46.43%), Postives = 63/84 (75.00%), Query Frame = 1


Query: 9   LKVETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDSTILIKKLKK 68
           L+  T VL+V + C+GC ++++K+L++I+GVY  +I+ +QQKVTV GNV+  ILIKK+ K
Sbjct: 27  LRYTTWVLRVSIHCEGCKRKIKKILSKIDGVYTTNIDVKQQKVTVIGNVEPEILIKKIMK 86

Query: 69  LGKHAELWPSTSKHGEGEESNLRK 93
            G+HAELWP++ ++    + N +K
Sbjct: 87  AGRHAELWPTSMENNINNDCNYQK 110

[Close]

BLAST of CSPI03G17490 vs. TAIR10
Match: AT1G23000.1 (AT1G23000.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 89.0 bits (219), Expect = 3.8e-18
Identity = 42/88 (47.73%), Postives = 58/88 (65.91%), Query Frame = 1


Query: 9  LKVETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDSTILIKKLKK 68
          LK++T  L+V++ C+GC ++V+KLL RIEGV  + I  E QKVTV+G+VDS  LI KL K
Sbjct: 10 LKIQTFSLRVNIHCEGCNKKVKKLLQRIEGVCHVKIEAEHQKVTVSGSVDSATLINKLVK 69

Query: 69 LGKHAELWPSTSKHGEGEESNLRKYIDN 97
           GKHAELW       + ++     +I N
Sbjct: 70 AGKHAELWSPNPNQNQPQKPKTNDFIKN 97

[Close]

BLAST of CSPI03G17490 vs. TAIR10
Match: AT5G37860.1 (AT5G37860.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 85.5 bits (210), Expect = 4.2e-17
Identity = 41/102 (40.20%), Postives = 64/102 (62.75%), Query Frame = 1


Query: 11  VETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDSTILIKKLKKLG 70
           ++T  LKV+++CQGC  +V+K L +IEGVY +DI+ +Q+ V V GN+D  IL+KKL K G
Sbjct: 9   LKTYFLKVNINCQGCKMKVKKTLRKIEGVYSVDIDTDQEAVIVRGNLDPEILVKKLNKRG 68

Query: 71  KHAELWPSTSKHGEGEESNLRKYIDNQMKDATDPYYTFQNQH 113
           KHA+L   T  H +    N +  +++  +   +  Y F + H
Sbjct: 69  KHAQLMFLTPYHKDQYFGNHQAVLNHDNRSLGNTQYNFGSNH 110

[Close]

BLAST of CSPI03G17490 vs. NCBI nr
Match: gi|700202300|gb|KGN57433.1| (hypothetical protein Csa_3G185130 [Cucumis sativus])

HSP 1 Score: 424.1 bits (1089), Expect = 1.4e-115
Identity = 204/204 (100.00%), Postives = 204/204 (100.00%), Query Frame = 1


Query: 1   MSKNPSHFLKVETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDST 60
           MSKNPSHFLKVETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDST
Sbjct: 1   MSKNPSHFLKVETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDST 60

Query: 61  ILIKKLKKLGKHAELWPSTSKHGEGEESNLRKYIDNQMKDATDPYYTFQNQHMLPILNRE 120
           ILIKKLKKLGKHAELWPSTSKHGEGEESNLRKYIDNQMKDATDPYYTFQNQHMLPILNRE
Sbjct: 61  ILIKKLKKLGKHAELWPSTSKHGEGEESNLRKYIDNQMKDATDPYYTFQNQHMLPILNRE 120

Query: 121 FNNRSLFERYLDQESGMSNSFRYHPVTTTAAQKARAVYDNEKLGNQMISMAHNVGIQDFQ 180
           FNNRSLFERYLDQESGMSNSFRYHPVTTTAAQKARAVYDNEKLGNQMISMAHNVGIQDFQ
Sbjct: 121 FNNRSLFERYLDQESGMSNSFRYHPVTTTAAQKARAVYDNEKLGNQMISMAHNVGIQDFQ 180

Query: 181 FDGVPDNTCVRDYRFGIFPDFPYA 205
           FDGVPDNTCVRDYRFGIFPDFPYA
Sbjct: 181 FDGVPDNTCVRDYRFGIFPDFPYA 204

[Close]

BLAST of CSPI03G17490 vs. NCBI nr
Match: gi|659114514|ref|XP_008457088.1| (PREDICTED: uncharacterized protein LOC103496852 [Cucumis melo])

HSP 1 Score: 392.1 bits (1006), Expect = 5.9e-106
Identity = 187/204 (91.67%), Postives = 193/204 (94.61%), Query Frame = 1


Query: 1   MSKNPSHFLKVETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDST 60
           MSKN SHFLKVET VLKVH+DCQGCL RVRKLLNRIEGVYKIDINCEQQKVTVTGNVDST
Sbjct: 1   MSKNCSHFLKVETHVLKVHIDCQGCLHRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDST 60

Query: 61  ILIKKLKKLGKHAELWPSTSKHGEGEESNLRKYIDNQMKDATDPYYTFQNQHMLPILNRE 120
           ILIKKL+KLGKHAELWPSTS HGEGEESN RKY+DNQMKD TDPYYTFQNQHM+PI NRE
Sbjct: 61  ILIKKLQKLGKHAELWPSTSTHGEGEESNPRKYLDNQMKDTTDPYYTFQNQHMIPIYNRE 120

Query: 121 FNNRSLFERYLDQESGMSNSFRYHPVTTTAAQKARAVYDNEKLGNQMISMAHNVGIQDFQ 180
           FNNRSLFERYLDQE GMSNSFRYHPVTTTAAQKARA YDNEKLGNQMISMAHNVGIQDFQ
Sbjct: 121 FNNRSLFERYLDQEGGMSNSFRYHPVTTTAAQKARAYYDNEKLGNQMISMAHNVGIQDFQ 180

Query: 181 FDGVPDNTCVRDYRFGIFPDFPYA 205
           FDGVPDN CVR ++FGIFPDFPYA
Sbjct: 181 FDGVPDNACVRVHQFGIFPDFPYA 204

[Close]

BLAST of CSPI03G17490 vs. NCBI nr
Match: gi|731417558|ref|XP_010660345.1| (PREDICTED: uncharacterized protein LOC104881553 [Vitis vinifera])

HSP 1 Score: 117.9 bits (294), Expect = 2.1e-23
Identity = 67/138 (48.55%), Postives = 87/138 (63.04%), Query Frame = 1


Query: 1   MSKNPSHFLKVETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDST 60
           MS +    LK++T VLKVHV+C GC Q+V+KLL+RIEGVY + I+ EQQKVTVTGNVD+ 
Sbjct: 1   MSTSGFQLLKIQTCVLKVHVNCNGCKQKVKKLLSRIEGVYSVSIDAEQQKVTVTGNVDAA 60

Query: 61  ILIKKLKKLGKHAELWPSTSKHGEGEE-SNLRKYIDNQMKDATDPYYTFQNQHMLP-ILN 120
            LI KL + GKHAELWP ++   + ++ SN  K  D+ +      Y    NQHMLP    
Sbjct: 61  TLINKLVRRGKHAELWPPSNHQNQNQQHSNFMKDDDHSIHPM--HYRINDNQHMLPSFYA 120

Query: 121 REFNNRSLFERYLDQESG 137
            E  +R     YL+Q  G
Sbjct: 121 MEDQDRWARGMYLNQSMG 136

[Close]

BLAST of CSPI03G17490 vs. NCBI nr
Match: gi|802762043|ref|XP_012089937.1| (PREDICTED: uncharacterized protein LOC105648226 [Jatropha curcas])

HSP 1 Score: 112.8 bits (281), Expect = 6.9e-22
Identity = 71/188 (37.77%), Postives = 106/188 (56.38%), Query Frame = 1


Query: 1   MSKNPSHFLKVETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDST 60
           M +N    LK+ET VLKVH++C+GC ++VRKLL +IEGVY +DI+ EQQ V V+G VDS+
Sbjct: 1   MLRNGHGILKIETHVLKVHINCEGCKEKVRKLLKKIEGVYSVDIDTEQQMVKVSGCVDSS 60

Query: 61  ILIKKLKKLGKHAELWPSTSKHGEGEES---NLRKYIDNQMKDATDPYYTFQNQHMLPIL 120
           ILIKKL K GK AE+W   SK+   EE    N ++++ N   +A+  +Y F    +    
Sbjct: 61  ILIKKLIKSGKKAEVWSPVSKYKLNEEQANINPKQFLAND-SNASKNHYMFPKSFI---- 120

Query: 121 NREFNNRSLFERYLDQESGMSNSFRYHPVTTTAAQKARAVYDNEKLGNQMI--SMAHNVG 180
             E  ++  FE++  Q   M     + PV          +YD+  +G+  +   MA  + 
Sbjct: 121 -NEIEDKGSFEKFPSQNIKMKAVDNHDPVVAKRMGN-NYMYDDIFIGDSRLEDDMAPLID 180

Query: 181 IQDFQFDG 184
             D+Q +G
Sbjct: 181 HGDYQENG 181

[Close]

BLAST of CSPI03G17490 vs. NCBI nr
Match: gi|296087184|emb|CBI33558.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 110.9 bits (276), Expect = 2.6e-21
Identity = 63/127 (49.61%), Postives = 81/127 (63.78%), Query Frame = 1


Query: 12  ETRVLKVHVDCQGCLQRVRKLLNRIEGVYKIDINCEQQKVTVTGNVDSTILIKKLKKLGK 71
           +T VLKVHV+C GC Q+V+KLL+RIEGVY + I+ EQQKVTVTGNVD+  LI KL + GK
Sbjct: 7   QTCVLKVHVNCNGCKQKVKKLLSRIEGVYSVSIDAEQQKVTVTGNVDAATLINKLVRRGK 66

Query: 72  HAELWPSTSKHGEGEE-SNLRKYIDNQMKDATDPYYTFQNQHMLP-ILNREFNNRSLFER 131
           HAELWP ++   + ++ SN  K  D+ +      Y    NQHMLP     E  +R     
Sbjct: 67  HAELWPPSNHQNQNQQHSNFMKDDDHSIHPM--HYRINDNQHMLPSFYAMEDQDRWARGM 126

Query: 132 YLDQESG 137
           YL+Q  G
Sbjct: 127 YLNQSMG 131

The following BLAST results are available for this feature:

BLAST of CSPI03G17490 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HIP21_ARATH	1.4e-11	40.28	Heavy metal-associated isoprenylated plant protein 21 OS=Arabidopsis thaliana GN...	[more]
CCH_ARATH	7.9e-10	43.94	Copper transport protein CCH OS=Arabidopsis thaliana GN=CCH PE=1 SV=1	[more]
HIP20_ARATH	8.7e-09	37.31	Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana GN...	[more]
HIP27_ARATH	2.5e-08	42.11	Heavy metal-associated isoprenylated plant protein 27 OS=Arabidopsis thaliana GN...	[more]
ATOX1_ARATH	3.3e-08	38.46	Copper transport protein ATX1 OS=Arabidopsis thaliana GN=ATX1 PE=1 SV=2	[more]

BLAST of CSPI03G17490 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L6R9_CUCSA	9.8e-116	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G185130 PE=4 SV=1	[more]
A0A067JFP2_JATCU	4.8e-22	37.77	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02499 PE=4 SV=1	[more]
D7TST6_VITVI	1.8e-21	49.61	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0006g01780 PE=4 SV=...	[more]
M5XDU3_PRUPE	5.3e-21	40.00	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007015mg PE=4 SV=1	[more]
F6HFJ4_VITVI	2.6e-20	36.08	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0011g04710 PE=4 SV=...	[more]

BLAST of CSPI03G17490 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G06130.1	4.4e-19	48.89	Heavy metal transport/detoxification superfamily protein	[more]
AT5G19090.1	7.6e-19	49.49	Heavy metal transport/detoxification superfamily protein	[more]
AT5G27690.1	1.3e-18	46.43	Heavy metal transport/detoxification superfamily protein	[more]
AT1G23000.1	3.8e-18	47.73	Heavy metal transport/detoxification superfamily protein	[more]
AT5G37860.1	4.2e-17	40.20	Heavy metal transport/detoxification superfamily protein	[more]

BLAST of CSPI03G17490 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700202300\|gb\|KGN57433.1\|	1.4e-115	100.00	hypothetical protein Csa_3G185130 [Cucumis sativus]	[more]
gi\|659114514\|ref\|XP_008457088.1\|	5.9e-106	91.67	PREDICTED: uncharacterized protein LOC103496852 [Cucumis melo]	[more]
gi\|731417558\|ref\|XP_010660345.1\|	2.1e-23	48.55	PREDICTED: uncharacterized protein LOC104881553 [Vitis vinifera]	[more]
gi\|802762043\|ref\|XP_012089937.1\|	6.9e-22	37.77	PREDICTED: uncharacterized protein LOC105648226 [Jatropha curcas]	[more]
gi\|296087184\|emb\|CBI33558.3\|	2.6e-21	49.61	unnamed protein product [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006121	HMA_dom

Vocabulary: **Biological Process**
Term	Definition
GO:0030001	metal ion transport

Vocabulary: **Molecular Function**
Term	Definition
GO:0046872	metal ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030001	metal ion transport
biological_process	GO:0009908	flower development
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046872	metal ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G17490.1	CSPI03G17490.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 17..72 score: 4.2
IPR006121	Heavy metal-associated domain, HMA	PROFILE	PS50846	HMA_2	coord: 20..75 score: 12
IPR006121	Heavy metal-associated domain, HMA	unknown	SSF55008	HMA, heavy metal-associated domain	coord: 11..77 score: 9.82
None	No IPR available	GENE3D	G3DSA:3.30.70.100		coord: 10..76 score: 6.9
None	No IPR available	PANTHER	PTHR22814	COPPER TRANSPORT PROTEIN ATOX1-RELATED	coord: 159..192 score: 4.2E-36coord: 1..101 score: 4.2
None	No IPR available	PANTHER	PTHR22814:SF150	SUBFAMILY NOT NAMED	coord: 1..101 score: 4.2E-36coord: 159..192 score: 4.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI03G17490	Cla013162	Watermelon (97103) v1	cpiwmB242
CSPI03G17490	Csa3G185130	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G17490	MELO3C020302	Melon (DHL92) v3.5.1	cpimeB241
CSPI03G17490	ClCG05G010850	Watermelon (Charleston Gray)	cpiwcgB237
CSPI03G17490	Lsi05G002940	Bottle gourd (USVL1VR-Ls)	cpilsiB209
CSPI03G17490	MELO3C020302.2	Melon (DHL92) v3.6.1	cpimedB239
CSPI03G17490	CsaV3_3G017660	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G17490	Cla97C05G093160	Watermelon (97103) v2	cpiwmbB221
CSPI03G17490	Bhi01G001338	Wax gourd	cpiwgoB283
CSPI03G17490	Cucsa.108340	Cucumber (Gy14) v1	cgycpiB133
CSPI03G17490	CmaCh01G003500	Cucurbita maxima (Rimu)	cmacpiB461
CSPI03G17490	CmoCh01G003680	Cucurbita moschata (Rifu)	cmocpiB449
CSPI03G17490	Cp4.1LG02g16840	Cucurbita pepo (Zucchini)	cpecpiB551
CSPI03G17490	CsGy3G017520	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G17490	Carg07440	Silver-seed gourd	carcpiB0799

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G17490	Silver-seed gourd	carcpiB0203
CSPI03G17490	Cucurbita maxima (Rimu)	cmacpiB247
CSPI03G17490	Cucurbita moschata (Rifu)	cmocpiB235
CSPI03G17490	Cucurbita pepo (Zucchini)	cpecpiB605