CSPI02G27600 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI02G27600
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein
Location	Chr2 : 23659647 .. 23660104 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCTTTTCTGAGAGCTTCAATGGTGTCAAAGAGAGAGAATTATTATCCCTTCAAAAGAGTGCCAAAAGTGGCTTCATGTTCTTGACTAGAGGCGCTCTGCCCATGCTGCCATTATGGAAGAGGTTCTTTGCTGACCATGACAAACTGTTCTCTATCCATGTGCATGCCCTTCCAGGCTATGAGCTAAATCTGTCCACGAGTTCTGTTTTCTACAGACGGGCAGATCCCCAGTCAGGTATTTCTTTTTCTTTCTTCAATCTGTTTGGTATTGATATTGTACCATGTTGAGTGTGAACACAAGTGGACATTACCTATTGATAGAAACATGTGTGAAGGAGTGATTTGTGAGGTCCACATTTTCTTTCACTTGTTTTTGTTTTGTAGCTATAGAGGTACTCCACATGGGCAGTAACAAGAACTCAATTAGAAAGTCATGCAACACTTGGAGACCTTAA

mRNA sequence

ATGAGCTTTTCTGAGAGCTTCAATGGTGTCAAAGAGAGAGAATTATTATCCCTTCAAAAGAGTGCCAAAAGTGGCTTCATGTTCTTGACTAGAGGCGCTCTGCCCATGCTGCCATTATGGAAGAGGTTCTTTGCTGACCATGACAAACTGTTCTCTATCCATGTGCATGCCCTTCCAGGCTATGAGCTAAATCTGTCCACGAGTTCTGTTTTCTACAGACGGGCAGATCCCCAGTCAGCTATAGAGGTACTCCACATGGGCAGTAACAAGAACTCAATTAGAAAGTCATGCAACACTTGGAGACCTTAA

Coding sequence (CDS)

ATGAGCTTTTCTGAGAGCTTCAATGGTGTCAAAGAGAGAGAATTATTATCCCTTCAAAAGAGTGCCAAAAGTGGCTTCATGTTCTTGACTAGAGGCGCTCTGCCCATGCTGCCATTATGGAAGAGGTTCTTTGCTGACCATGACAAACTGTTCTCTATCCATGTGCATGCCCTTCCAGGCTATGAGCTAAATCTGTCCACGAGTTCTGTTTTCTACAGACGGGCAGATCCCCAGTCAGCTATAGAGGTACTCCACATGGGCAGTAACAAGAACTCAATTAGAAAGTCATGCAACACTTGGAGACCTTAA

Homology

[Close]

BLAST of CSPI02G27600 vs. TrEMBL
Match: A0A0A0LNA6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G435490 PE=4 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 9.4e-51
Identity = 100/102 (98.04%), Postives = 100/102 (98.04%), Query Frame = 1


Query: 1   MSFSESFNGVKERELLSLQKSAKSGFMFLTRGALPMLPLWKRFFADHDKLFSIHVHALPG 60
           MSFSESFNGVKERELLSLQKSAKSGFMFLTRG LPMLPLWKRFFADHDKLFSIHVHALPG
Sbjct: 6   MSFSESFNGVKERELLSLQKSAKSGFMFLTRGPLPMLPLWKRFFADHDKLFSIHVHALPG 65

Query: 61  YELNLSTSSVFYRRADPQSAIEVLHMGSNKNSIRKSCNTWRP 103
           YELNLSTSSVFYRRADPQSAIEVLHM SNKNSIRKSCNTWRP
Sbjct: 66  YELNLSTSSVFYRRADPQSAIEVLHMDSNKNSIRKSCNTWRP 107

[Close]

BLAST of CSPI02G27600 vs. TrEMBL
Match: A0A0A0L2Q4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G110720 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 8.0e-18
Identity = 44/63 (69.84%), Postives = 52/63 (82.54%), Query Frame = 1


Query: 19  QKSAKSGFMFLTRGALPMLPLWKRFFADHDKLFSIHVHALPGYELNLSTSSVFYRRADPQ 78
           ++  K  FMFLTRG LPMLPLW+RFFA H+KLFSI+VHALPGY+LN+STSSVFYRR  P 
Sbjct: 121 ERVPKVAFMFLTRGPLPMLPLWERFFAGHEKLFSIYVHALPGYKLNVSTSSVFYRRQIPS 180

Query: 79  SAI 82
             +
Sbjct: 181 QRV 183

[Close]

BLAST of CSPI02G27600 vs. TrEMBL
Match: W9RJ51_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_010966 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.3e-15
Identity = 41/72 (56.94%), Postives = 54/72 (75.00%), Query Frame = 1


Query: 10  VKERELLSLQKSAKSGFMFLTRGALPMLPLWKRFFADHDKLFSIHVHALPGYELNLSTSS 69
           V ++E    ++  K  FMFLTRGALP+LPLW+R F  H+KLFSI+VHALPGY+LN+ST+S
Sbjct: 113 VPQKEEYPYKRVPKVAFMFLTRGALPLLPLWERLFRGHEKLFSIYVHALPGYKLNVSTTS 172

Query: 70  VFYRRADPQSAI 82
            F+RR  P   +
Sbjct: 173 PFHRREIPSQHV 184

[Close]

BLAST of CSPI02G27600 vs. TrEMBL
Match: F6HK07_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0035g01710 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 2.4e-14
Identity = 38/73 (52.05%), Postives = 50/73 (68.49%), Query Frame = 1


Query: 10  VKERELLSLQKSAKSGFMFLTRGALPMLPLWKRFFADHDKLFSIHVHALPGYELNLSTSS 69
           V  R     ++  K  FMFLTRG LP++PLW+RFF  H K FS++VHALPG+ELN+S  S
Sbjct: 94  VPRRAEYPFKRVPKVAFMFLTRGPLPLMPLWERFFEGHRKYFSVYVHALPGFELNVSMHS 153

Query: 70  VFYRRADPQSAIE 83
            FY+R  P  ++E
Sbjct: 154 AFYKRQIPSKSVE 166

[Close]

BLAST of CSPI02G27600 vs. TrEMBL
Match: V4T5B9_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10001419mg PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 5.4e-14
Identity = 38/68 (55.88%), Postives = 50/68 (73.53%), Query Frame = 1


Query: 10  VKERELLSLQKSAKSGFMFLTRGALPMLPLWKRFFADHDKLFSIHVHALPGYELNLSTSS 69
           V  +E    +++ +  FMFLTRG LP+LPLW+RFF  H+K FSI+VHALPGY+LN+S+ S
Sbjct: 110 VPYKEEYPFERTPRVAFMFLTRGPLPLLPLWERFFKGHEKYFSIYVHALPGYKLNVSSDS 169

Query: 70  VFYRRADP 78
            FY R  P
Sbjct: 170 PFYGRQIP 177

[Close]

BLAST of CSPI02G27600 vs. TAIR10
Match: AT1G10280.1 (AT1G10280.1 Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein)

HSP 1 Score: 75.9 bits (185), Expect = 1.7e-14
Identity = 34/73 (46.58%), Postives = 46/73 (63.01%), Query Frame = 1


Query: 10  VKERELLSLQKSAKSGFMFLTRGALPMLPLWKRFFADHDKLFSIHVHALPGYELNLSTSS 69
           V  +E     +  K  FMFLTRG LPMLPLW++FF  ++K  S++VH  PGY++N+S  S
Sbjct: 130 VPVKEEYPYDRVPKVAFMFLTRGPLPMLPLWEKFFKGNEKYLSVYVHTPPGYDMNVSRDS 189

Query: 70  VFYRRADPQSAIE 83
            FY R  P   +E
Sbjct: 190 PFYDRQIPSQRVE 202

[Close]

BLAST of CSPI02G27600 vs. TAIR10
Match: AT3G21310.1 (AT3G21310.1 Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein)

HSP 1 Score: 72.8 bits (177), Expect = 1.4e-13
Identity = 31/59 (52.54%), Postives = 39/59 (66.10%), Query Frame = 1


Query: 23  KSGFMFLTRGALPMLPLWKRFFADHDKLFSIHVHALPGYELNLSTSSVFYRRADPQSAI 82
           K  FMFLT+G LP  PLW+RFF  H+  +SI+VH LP Y  +  +SSVFYRR  P   +
Sbjct: 115 KMAFMFLTKGPLPFAPLWERFFKGHEGFYSIYVHTLPNYRSDFPSSSVFYRRQIPSQHV 173

[Close]

BLAST of CSPI02G27600 vs. TAIR10
Match: AT1G51770.1 (AT1G51770.1 Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein)

HSP 1 Score: 72.0 bits (175), Expect = 2.4e-13
Identity = 32/70 (45.71%), Postives = 45/70 (64.29%), Query Frame = 1


Query: 12  ERELLSLQKSAKSGFMFLTRGALPMLPLWKRFFADHDKLFSIHVHALPGYELNLSTSSVF 71
           +R     ++  K  FMFL +G LP  PLW++F   H+ L+SI+VH+LP Y+ + S SSVF
Sbjct: 113 QRNGYPFRRVPKLAFMFLAKGPLPFAPLWEKFCKGHEGLYSIYVHSLPSYKSDFSRSSVF 172

Query: 72  YRRADPQSAI 82
           YRR  P  A+
Sbjct: 173 YRRYIPSQAV 182

[Close]

BLAST of CSPI02G27600 vs. TAIR10
Match: AT1G10880.1 (AT1G10880.1 Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein)

HSP 1 Score: 62.8 bits (151), Expect = 1.5e-10
Identity = 28/60 (46.67%), Postives = 37/60 (61.67%), Query Frame = 1


Query: 23  KSGFMFLTRGALPMLPLWKRFFADHDKLFSIHVHALPGYELNLSTSSVFYRRADPQSAIE 82
           K  FMFLTR  LP+ PLW+ FF  H+  +SI+VH  P +      SSVFY++  P  A+E
Sbjct: 118 KVAFMFLTRWNLPLSPLWEMFFKGHEGFYSIYVHTSPEFTQEPPESSVFYKKRIPSKAVE 177

[Close]

BLAST of CSPI02G27600 vs. TAIR10
Match: AT1G68380.1 (AT1G68380.1 Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein)

HSP 1 Score: 62.8 bits (151), Expect = 1.5e-10
Identity = 28/60 (46.67%), Postives = 37/60 (61.67%), Query Frame = 1


Query: 23  KSGFMFLTRGALPMLPLWKRFFADHDKLFSIHVHALPGYELNLSTSSVFYRRADPQSAIE 82
           K  FMFLT G LP+ PLW+RFF  H+ LF+I+VH    Y+  +   SVFY R  P   ++
Sbjct: 124 KVAFMFLTWGPLPLAPLWERFFRGHEGLFTIYVHTNSSYDEFMPQDSVFYGRRIPSKRVD 183

[Close]

BLAST of CSPI02G27600 vs. NCBI nr
Match: gi|700208266|gb|KGN63385.1| (hypothetical protein Csa_2G435490 [Cucumis sativus])

HSP 1 Score: 207.2 bits (526), Expect = 1.4e-50
Identity = 100/102 (98.04%), Postives = 100/102 (98.04%), Query Frame = 1


Query: 1   MSFSESFNGVKERELLSLQKSAKSGFMFLTRGALPMLPLWKRFFADHDKLFSIHVHALPG 60
           MSFSESFNGVKERELLSLQKSAKSGFMFLTRG LPMLPLWKRFFADHDKLFSIHVHALPG
Sbjct: 6   MSFSESFNGVKERELLSLQKSAKSGFMFLTRGPLPMLPLWKRFFADHDKLFSIHVHALPG 65

Query: 61  YELNLSTSSVFYRRADPQSAIEVLHMGSNKNSIRKSCNTWRP 103
           YELNLSTSSVFYRRADPQSAIEVLHM SNKNSIRKSCNTWRP
Sbjct: 66  YELNLSTSSVFYRRADPQSAIEVLHMDSNKNSIRKSCNTWRP 107

[Close]

BLAST of CSPI02G27600 vs. NCBI nr
Match: gi|659102622|ref|XP_008452226.1| (PREDICTED: uncharacterized protein LOC103493307 isoform X2 [Cucumis melo])

HSP 1 Score: 99.0 bits (245), Expect = 5.2e-18
Identity = 46/71 (64.79%), Postives = 52/71 (73.24%), Query Frame = 1


Query: 11  KERELLSLQKSAKSGFMFLTRGALPMLPLWKRFFADHDKLFSIHVHALPGYELNLSTSSV 70
           K       ++  K  FMFLTRG LPMLPLW+RFFA HD LFSI+VHALPGYELN+STSSV
Sbjct: 108 KRENYYPFKRVPKVAFMFLTRGPLPMLPLWERFFAGHDNLFSIYVHALPGYELNVSTSSV 167

Query: 71  FYRRADPQSAI 82
           FYRR  P   +
Sbjct: 168 FYRRQIPSQRV 178

[Close]

BLAST of CSPI02G27600 vs. NCBI nr
Match: gi|659102620|ref|XP_008452225.1| (PREDICTED: uncharacterized protein LOC103493307 isoform X1 [Cucumis melo])

HSP 1 Score: 99.0 bits (245), Expect = 5.2e-18
Identity = 46/71 (64.79%), Postives = 52/71 (73.24%), Query Frame = 1


Query: 11  KERELLSLQKSAKSGFMFLTRGALPMLPLWKRFFADHDKLFSIHVHALPGYELNLSTSSV 70
           K       ++  K  FMFLTRG LPMLPLW+RFFA HD LFSI+VHALPGYELN+STSSV
Sbjct: 113 KRENYYPFKRVPKVAFMFLTRGPLPMLPLWERFFAGHDNLFSIYVHALPGYELNVSTSSV 172

Query: 71  FYRRADPQSAI 82
           FYRR  P   +
Sbjct: 173 FYRRQIPSQRV 183

[Close]

BLAST of CSPI02G27600 vs. NCBI nr
Match: gi|778676402|ref|XP_011650576.1| (PREDICTED: uncharacterized protein LOC101215808 isoform X1 [Cucumis sativus])

HSP 1 Score: 97.8 bits (242), Expect = 1.2e-17
Identity = 44/63 (69.84%), Postives = 52/63 (82.54%), Query Frame = 1


Query: 19  QKSAKSGFMFLTRGALPMLPLWKRFFADHDKLFSIHVHALPGYELNLSTSSVFYRRADPQ 78
           ++  K  FMFLTRG LPMLPLW+RFFA H+KLFSI+VHALPGY+LN+STSSVFYRR  P 
Sbjct: 121 ERVPKVAFMFLTRGPLPMLPLWERFFAGHEKLFSIYVHALPGYKLNVSTSSVFYRRQIPS 180

Query: 79  SAI 82
             +
Sbjct: 181 QRV 183

[Close]

BLAST of CSPI02G27600 vs. NCBI nr
Match: gi|778676405|ref|XP_011650577.1| (PREDICTED: uncharacterized protein LOC101215808 isoform X2 [Cucumis sativus])

HSP 1 Score: 97.8 bits (242), Expect = 1.2e-17
Identity = 44/63 (69.84%), Postives = 52/63 (82.54%), Query Frame = 1


Query: 19  QKSAKSGFMFLTRGALPMLPLWKRFFADHDKLFSIHVHALPGYELNLSTSSVFYRRADPQ 78
           ++  K  FMFLTRG LPMLPLW+RFFA H+KLFSI+VHALPGY+LN+STSSVFYRR  P 
Sbjct: 116 ERVPKVAFMFLTRGPLPMLPLWERFFAGHEKLFSIYVHALPGYKLNVSTSSVFYRRQIPS 175

Query: 79  SAI 82
             +
Sbjct: 176 QRV 178

The following BLAST results are available for this feature:

BLAST of CSPI02G27600 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of CSPI02G27600 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LNA6_CUCSA	9.4e-51	98.04	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G435490 PE=4 SV=1	[more]
A0A0A0L2Q4_CUCSA	8.0e-18	69.84	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G110720 PE=4 SV=1	[more]
W9RJ51_9ROSA	1.3e-15	56.94	Uncharacterized protein OS=Morus notabilis GN=L484_010966 PE=4 SV=1	[more]
F6HK07_VITVI	2.4e-14	52.05	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0035g01710 PE=4 SV=...	[more]
V4T5B9_9ROSI	5.4e-14	55.88	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10001419mg PE=4 SV=1	[more]

BLAST of CSPI02G27600 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G10280.1	1.7e-14	46.58	Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family p...	[more]
AT3G21310.1	1.4e-13	52.54	Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family p...	[more]
AT1G51770.1	2.4e-13	45.71	Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family p...	[more]
AT1G10880.1	1.5e-10	46.67	Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family p...	[more]
AT1G68380.1	1.5e-10	46.67	Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family p...	[more]

BLAST of CSPI02G27600 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700208266\|gb\|KGN63385.1\|	1.4e-50	98.04	hypothetical protein Csa_2G435490 [Cucumis sativus]	[more]
gi\|659102622\|ref\|XP_008452226.1\|	5.2e-18	64.79	PREDICTED: uncharacterized protein LOC103493307 isoform X2 [Cucumis melo]	[more]
gi\|659102620\|ref\|XP_008452225.1\|	5.2e-18	64.79	PREDICTED: uncharacterized protein LOC103493307 isoform X1 [Cucumis melo]	[more]
gi\|778676402\|ref\|XP_011650576.1\|	1.2e-17	69.84	PREDICTED: uncharacterized protein LOC101215808 isoform X1 [Cucumis sativus]	[more]
gi\|778676405\|ref\|XP_011650577.1\|	1.2e-17	69.84	PREDICTED: uncharacterized protein LOC101215808 isoform X2 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR003406	Glyco_trans_14

Vocabulary: **Molecular Function**
Term	Definition
GO:0008375	acetylglucosaminyltransferase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0016020	membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008375	acetylglucosaminyltransferase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G27600.1	CSPI02G27600.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003406	Glycosyl transferase, family 14	PFAM	PF02485	Branch	coord: 26..79 score: 1.2
None	No IPR available	PANTHER	PTHR31042	FAMILY NOT NAMED	coord: 26..82 score: 1.1
None	No IPR available	PANTHER	PTHR31042:SF3	SUBFAMILY NOT NAMED	coord: 26..82 score: 1.1

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI02G27600	Csa2G435490	Cucumber (Chinese Long) v2	cpicuB065
CSPI02G27600	CsaV3_2G036250	Cucumber (Chinese Long) v3	cpicucB081
CSPI02G27600	CsGy2G027040	Cucumber (Gy14) v2	cgybcpiB057

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI02G27600	Melon (DHL92) v3.6.1	cpimedB088
CSPI02G27600	Silver-seed gourd	carcpiB0457
CSPI02G27600	Silver-seed gourd	carcpiB1089
CSPI02G27600	Watermelon (97103) v2	cpiwmbB170
CSPI02G27600	Wax gourd	cpiwgoB171
CSPI02G27600	Cucumber (Gy14) v1	cgycpiB481
CSPI02G27600	Cucurbita maxima (Rimu)	cmacpiB549
CSPI02G27600	Cucurbita maxima (Rimu)	cmacpiB595
CSPI02G27600	Cucurbita moschata (Rifu)	cmocpiB538
CSPI02G27600	Cucurbita moschata (Rifu)	cmocpiB584
CSPI02G27600	Melon (DHL92) v3.5.1	cpimeB096
CSPI02G27600	Watermelon (Charleston Gray)	cpiwcgB098
CSPI02G27600	Watermelon (97103) v1	cpiwmB107
CSPI02G27600	Cucurbita pepo (Zucchini)	cpecpiB272
CSPI02G27600	Cucurbita pepo (Zucchini)	cpecpiB708
CSPI02G27600	Bottle gourd (USVL1VR-Ls)	cpilsiB092