CSPI02G03810 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI02G03810
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	E3 ubiquitin-protein ligase Topors
Location	Chr2 : 2665291 .. 2669348 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTGTCGTTCACTGTAAACCCATGTCGGGTAAGTCCATGGAATTGCAGAGCACTGCATCAATGGAGATGGAGACAAGAACTTCCACTTCGGACTATACGAACCCTTGCCCGATCTGCCTTGGACCCATAAATCAATCTTCCTATCTCGACAAGTGCTTTCGTAAGTTTTTATTTCTCTTAATCTGATCTTTCTTTCGTTTTTCTTAAATTTTCCTGTCATCTAAATAAAAATTGGTTTAGAAAAATTATTCATTTTTATGAATTGGGGTTTCTTTTTTCCGATTCCTGTAGTGACCCATTTGGAAATATTCCTTTATGTGTTACTGTATCAACAGAAGAACTAAGAATCACTCTCTTCTCCTTTTATTAGGAGATTTTAGCTTTTAGCTTTTAGCTCTTCCCCCAATTATCAAGGTTTCTGCCACTGTTATTCGTATTTGATTAACGAAATTGTTTGATGATTATAGTATCTTGTTTGATAATTAACTGAATTCATTTGGTATAGAGATGAACTAGCTAATTCCTTAGCTTTGAGCGTATAAAAAGTTCTTCTGGAGATGCTTAATTTGATTTAATATACTCAGTTCTTTAGTGGCTGTATCCTTTAGGCAAGGAATTTTGCGTGTTAATATGGCTTCATCTTCTTCGATGAAATGGAAAACAGAAACAGCAGCGAGAATGCTTTAGTTTTCTTTGATTTGTTGAATTTGTAATCATTGATGAACTCCTTCACTGTCTTTGAGATGAATATGTTCATTTAATTTATTTTTAATATACTTGCTTATATCTAGGCATGAATATTAGTTTGGTTTATGTTGTGTGTAATGCATGGAATATCGAAATGAAAGAGGAAATATCAGATTTTACATTTGGTATCATGTTGATTACCAGTAATTTCCAAAGTTTAAACCTTGAAGTTTTGGTGAATTTGATCATTTAGTTAACTCTAAAGAATCTATTAGTTCATTCATGTTGAGTACAATGTACCATTTAGAAAAAGAGGAATGTTAAGAAGTTTGAATGGTTTTAGCTTTCAACTCTATTTATATGCCTTCCAGATAACTTTTGTTACAATTGCATTGTACAATGGACCAAAGTGGTTTCTGGGAAGCGCTCCTGCACACTTTCCTCCATTAAATGTCCTTTATGCAAGGTTCAATTTTTCTTCCCCCCGTTAATCTTGTTTTATTTTAGCATTCCATACTTCTAGTATTGCATTTCTTATTTTTTTTTTATAACTAATCACCAGACAGAAAGTTCTTCTATAATACATGGATTAGATGGACACAATTTCCAAAGACATTATGTTAATCCGGATTTCCAGGATAGGTATCTTCCTTTTGACATTTCCCCTAGTTCTTCTATATTTATTTTCTCTACCTTAATTGACTACGGCTATTATTAATGTTATTTGCAGCTTTATCTTATCAAAAGCTCATAGGTATAGATTACAGTGCTATTATACTGAACCAGGTTCGTTTTTTTGTTTATCCTTCAACTTATTATGTGATGATGCTCCAACATTTACATCCAGTTACTCGGATTTATGAATTTCCCTAATTGTCTACATGTAGGTTTCTTGAATGACATATTTGATGTACAGAGGTATTGGAAGTTACAGAAGTATCTCCAGGCAAACCAGTGGCTTGAAGTTTGGTTGAAAAGGGAACTTCAAGCTTTGATTCAGGTTAGTGTCTTATTTTCTTTCATCACATTTGTACTTCTATGGGATATTGCTTCCTTTGGCCCCTTTTTTCTATGGAGAATTGTTTGCATCCAATATCAAACAACATTCTAAGAGTCAAGACCAAATAGGTGGATGGGAAAGGGAGGGAGCTCTTCATTCTTACAGCCGGATTCTTGGGGAAATGAATTCTATCACTGCTCCTAGTAGTCTGTTAACGCCCATCAATAAATGGAGCACAAGACAAGCTAGAAAGTATTTTTTCTGCAAATCATCCCAAGGGTTTCTAACGCAGGCATCCAACTATCCAAGGAATTGGTATCCTATTGAAACATAGAAACCATTGGCTTGTGTCGCCTACTTGCTTCAAGCATATATGGAATGGCTGATCATTCATTGAGTTCATGAGTTTTTTAAGACCTCACTAATCAGGCTTACACTTTTGAGCACATAATGAATGAAATTCACCCCGGATGAAGATTGAATCTCCCCTCCGCATAGGCATGAAAAAAGGTAGGACAAGGATGGAGTTTCTCCCATTTGTGGCATAGTGGGCCGTAGGATGGGTTGTCCTTCTAAGGATGAGGGAGGGGGTTTGTTTATTGATTGGGGATTTAAGAACTTTATTTTATTTCAATGGTGTGGAAACCTGTGTGTATTGTGGAAGTAGACAAGATGAGGTAAAGATGTGTCACTTTCTTTAACTGTGAGGAATTGAGAACTCTGTTTTCGTAGAAAATGGATTACTGTATACAAAACGAGATAGCGGTAGTAGATTTATGTTACTGTTTCTATTGCATAGTTTCTTGATTTATCTATTCTGTTTCTCAGCAGTACAGTTCATTGTTGATGATGACTGATAGTGTTTTATTCACTTTTACAGGAAGAAGATGTTGACATAATTATGCATCACTTTCTTGGTTTAATTAACTCATTTTTTCGTAGGTAATACTAATAATCATCCATCATTGCATTCTATACTTTGGTCAAATAAAAAATTGGTTTAAAACTCCAATTTCGTGGATAGTCCCTAAATTTTAGTTTGTAATAAGTCCCTGTGAGCAGTTTTTATAATATAGTGTCCATATCATAAAAGTCTTGTAAAAAATTAATGGAATTTCTTACCCAAGTATTTGCAATTGGTAGCACTTTTGGAGTTAATTGTGTGTATAACAACATTTTTAAAGAATTGCAAAATTTATCAATGCTAGACTCTTTCGACGATCATTGATAGACTCTGATCAATGACATAGTTAAAAGGCACTGAGAGCTTATCTAAATTTTGCTATATCTACAAATTCTTTCGCATTTTGTGCTATATCTTGGGATATTTTAGATCAGATTGTTATATTTGCAACTTGATAAACTAATTAGGGATCAGATGTATAAGTTACAAAGATTAAGTTTAAGTTGTTACCTAATAAAAATTGACGCTTACTTTTACTAAATCGTTATGACAGGTACAAAGACTAAATTGTTACGTACTAAAGATTAAATGACTAAGTTGTTACACGTTTGAAAGTAGATAGACAAAATTGTTACACACTAGAGAGTTAGGGCTGATCTTTTTTCTTTTTCTTCAATGCTATTCATTCTGGCTTGACATGTAGAATTGTTCAAAACTCATTTTCATTCATCTTTCTTACTACGACCTATCCCCTATCCACATGAATGTAAATATGCATGGATATGATAACTGATTACACTCTGAAATCTGCTTCTTGTTCTTTGTTTTTTCTCCTCAAAGAAATGAACCTAAGTATCAAACAGAAACTCCTGAACTGAAGAGGAAGAGGTTTAGCCAAACGATTCTTGATGCAGCAAAGCCATTCCTTTCAGCAAGAGCAGATCGATTCATTCTCGAGTTGGAGCTATTTCTTGCTTCAGGTTTGAACATTGAAGCCTATGATTCAGTTTACTTGCAACGGTTGGGTTGGAACAAGCCTATAGTGCCGAGCGTGGCTAACGAAGAAGATCTTGGACTCAAATCTGTAACTCCATACTTGTATATCTTTGATTGTGACCCTGATGATGGTGATTAAACAAGCTCTTACAAATTGTTATTGTTTTTGTGAAGGCAAGTCTTGTGTTATCTCCCCACCTTCCAGTATGAGTTTAATTCAAAGTTGGAATCTGTCAAGCTGTCTGGTTTAACTTTTCGTTGTTGAATTTGAATTTCGATAATCTTGTAACTACATATTAATACTTCTCTTATTGAGATTGGCTGCTTACTAGACCCATCAACACAGACAGATAAATGGTCAATTCGGGTTTGGTTTTGGCCAAATTCGATTCTATGTCAATTGTATAAAAGAGAAACTAAATAGATATTAAATGGATGAAATTTGTGTAATGATAACCTAATCTTAGAGGC

mRNA sequence

ATGTCGGGTAAGTCCATGGAATTGCAGAGCACTGCATCAATGGAGATGGAGACAAGAACTTCCACTTCGGACTATACGAACCCTTGCCCGATCTGCCTTGGACCCATAAATCAATCTTCCTATCTCGACAAGTGCTTTCATAACTTTTGTTACAATTGCATTGTACAATGGACCAAAGTGGTTTCTGGGAAGCGCTCCTGCACACTTTCCTCCATTAAATGTCCTTTATGCAAGACAGAAAGTTCTTCTATAATACATGGATTAGATGGACACAATTTCCAAAGACATTATGTTAATCCGGATTTCCAGGATAGCTTTATCTTATCAAAAGCTCATAGGTATAGATTACAGTGCTATTATACTGAACCAGGTTTCTTGAATGACATATTTGATGTACAGAGGTATTGGAAGTTACAGAAGTATCTCCAGGCAAACCAGTGGCTTGAAGTTTGGTTGAAAAGGGAACTTCAAGCTTTGATTCAGGAAGAAGATGTTGACATAATTATGCATCACTTTCTTGGTTTAATTAACTCATTTTTTCGTAGAAATGAACCTAAGTATCAAACAGAAACTCCTGAACTGAAGAGGAAGAGGTTTAGCCAAACGATTCTTGATGCAGCAAAGCCATTCCTTTCAGCAAGAGCAGATCGATTCATTCTCGAGTTGGAGCTATTTCTTGCTTCAGGTTTGAACATTGAAGCCTATGATTCAGTTTACTTGCAACGGTTGGGTTGGAACAAGCCTATAGTGCCGAGCGTGGCTAACGAAGAAGATCTTGGACTCAAATCTGTAACTCCATACTTGTATATCTTTGATTGTGACCCTGATGATGGTGATTAA

Coding sequence (CDS)

ATGTCGGGTAAGTCCATGGAATTGCAGAGCACTGCATCAATGGAGATGGAGACAAGAACTTCCACTTCGGACTATACGAACCCTTGCCCGATCTGCCTTGGACCCATAAATCAATCTTCCTATCTCGACAAGTGCTTTCATAACTTTTGTTACAATTGCATTGTACAATGGACCAAAGTGGTTTCTGGGAAGCGCTCCTGCACACTTTCCTCCATTAAATGTCCTTTATGCAAGACAGAAAGTTCTTCTATAATACATGGATTAGATGGACACAATTTCCAAAGACATTATGTTAATCCGGATTTCCAGGATAGCTTTATCTTATCAAAAGCTCATAGGTATAGATTACAGTGCTATTATACTGAACCAGGTTTCTTGAATGACATATTTGATGTACAGAGGTATTGGAAGTTACAGAAGTATCTCCAGGCAAACCAGTGGCTTGAAGTTTGGTTGAAAAGGGAACTTCAAGCTTTGATTCAGGAAGAAGATGTTGACATAATTATGCATCACTTTCTTGGTTTAATTAACTCATTTTTTCGTAGAAATGAACCTAAGTATCAAACAGAAACTCCTGAACTGAAGAGGAAGAGGTTTAGCCAAACGATTCTTGATGCAGCAAAGCCATTCCTTTCAGCAAGAGCAGATCGATTCATTCTCGAGTTGGAGCTATTTCTTGCTTCAGGTTTGAACATTGAAGCCTATGATTCAGTTTACTTGCAACGGTTGGGTTGGAACAAGCCTATAGTGCCGAGCGTGGCTAACGAAGAAGATCTTGGACTCAAATCTGTAACTCCATACTTGTATATCTTTGATTGTGACCCTGATGATGGTGATTAA

Homology

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BLAST of CSPI02G03810 vs. Swiss-Prot
Match: TOPRS_MOUSE (E3 ubiquitin-protein ligase Topors OS=Mus musculus GN=Topors PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 3.4e-11
Identity = 31/94 (32.98%), Postives = 48/94 (51.06%), Query Frame = 1


Query: 29  CPICLGPINQSSYLDKCFHNFCYNCIVQWTKVVSGKRSCTLSSIKCPLCKTESSSIIHGL 88
           CPICL   +  SYLD+C H FC+ C+ +W+K          +  +CPLCK    SI H +
Sbjct: 104 CPICLDRFDNVSYLDRCLHKFCFRCVQEWSK----------NKAECPLCKQPFDSIFHSV 163

Query: 89  DGH-NFQRHYVNPDFQDSFILSKAHRYRLQCYYT 122
               +F+ + + P +  SF   +  R+R +   T
Sbjct: 164 RAEDDFKEYVLRPSYNGSFTNPEVRRFRYRTTMT 187

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BLAST of CSPI02G03810 vs. Swiss-Prot
Match: TOPRS_HUMAN (E3 ubiquitin-protein ligase Topors OS=Homo sapiens GN=TOPORS PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 7.5e-11
Identity = 31/89 (34.83%), Postives = 47/89 (52.81%), Query Frame = 1


Query: 29  CPICLGPINQSSYLDKCFHNFCYNCIVQWTKVVSGKRSCTLSSIKCPLCKTESSSIIHGL 88
           CPICL   +  SYLD+C H FC+ C+ +W+K          +  +CPLCK    SI H +
Sbjct: 103 CPICLDRFDNVSYLDRCLHKFCFRCVQEWSK----------NKAECPLCKQPFDSIFHSV 162

Query: 89  DGH-NFQRHYVNPDFQDSFIL-SKAHRYR 116
               +F+ + + P +  SF+   +  RYR
Sbjct: 163 RAEDDFKEYVLRPSYNGSFVTPDRRFRYR 181

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BLAST of CSPI02G03810 vs. TrEMBL
Match: A0A0A0LLN5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021620 PE=4 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 6.5e-163
Identity = 278/279 (99.64%), Postives = 279/279 (100.00%), Query Frame = 1


Query: 1   MSGKSMELQSTASMEMETRTSTSDYTNPCPICLGPINQSSYLDKCFHNFCYNCIVQWTKV 60
           MSGKSMELQSTASMEMETRTSTSDYTNPCPICLGPINQSSYLDKCFHNFCYNCIVQWTKV
Sbjct: 1   MSGKSMELQSTASMEMETRTSTSDYTNPCPICLGPINQSSYLDKCFHNFCYNCIVQWTKV 60

Query: 61  VSGKRSCTLSSIKCPLCKTESSSIIHGLDGHNFQRHYVNPDFQDSFILSKAHRYRLQCYY 120
           VSGKRSCTLSSIKCPLCKTESSSIIHGLDGHNFQRHYVNPDFQDSFILSKAHRYRLQCYY
Sbjct: 61  VSGKRSCTLSSIKCPLCKTESSSIIHGLDGHNFQRHYVNPDFQDSFILSKAHRYRLQCYY 120

Query: 121 TEPGFLNDIFDVQRYWKLQKYLQANQWLEVWLKRELQALIQEEDVDIIMHHFLGLINSFF 180
           TEPGFLNDIFDVQRYWKLQKYLQANQWLEVWLKRELQALIQEEDVDIIMHHFLGLINSFF
Sbjct: 121 TEPGFLNDIFDVQRYWKLQKYLQANQWLEVWLKRELQALIQEEDVDIIMHHFLGLINSFF 180

Query: 181 RRNEPKYQTETPELKRKRFSQTILDAAKPFLSARADRFILELELFLASGLNIEAYDSVYL 240
           RRNEP+YQTETPELKRKRFSQTILDAAKPFLSARADRFILELELFLASGLNIEAYDSVYL
Sbjct: 181 RRNEPEYQTETPELKRKRFSQTILDAAKPFLSARADRFILELELFLASGLNIEAYDSVYL 240

Query: 241 QRLGWNKPIVPSVANEEDLGLKSVTPYLYIFDCDPDDGD 280
           QRLGWNKPIVPSVANEEDLGLKSVTPYLYIFDCDPDDGD
Sbjct: 241 QRLGWNKPIVPSVANEEDLGLKSVTPYLYIFDCDPDDGD 279

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BLAST of CSPI02G03810 vs. TrEMBL
Match: M5WCB8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009982mg PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 3.6e-97
Identity = 172/265 (64.91%), Postives = 211/265 (79.62%), Query Frame = 1


Query: 13  SMEMETRTSTSDYTNPCPICLGPINQSSYLDKCFHNFCYNCIVQWTKVVSGKRSCTLSSI 72
           ++   T +S+S+ +NPCPICLGPI Q SYLDKCFH FCYNCI++WTKVV+ K+SC  +S+
Sbjct: 5   NVNKNTSSSSSEDSNPCPICLGPIIQDSYLDKCFHKFCYNCILRWTKVVAHKQSCLPTSV 64

Query: 73  KCPLCKTESSSIIHGLDGHNFQRHYVNPDFQDSFILSKAHRYRLQCYYTEPGFLNDIFDV 132
           KCPLCKTE+ SIIHG DG +FQ+HY+N D    FILSK H+YRLQCYYTEPGFLNDIFDV
Sbjct: 65  KCPLCKTENLSIIHGYDGSSFQKHYINQD--SGFILSKDHKYRLQCYYTEPGFLNDIFDV 124

Query: 133 QRYWKLQKYLQANQWLEVWLKRELQALIQEEDVDIIMHHFLGLINSFFRRNEPKYQTETP 192
            RYWK  KYLQ N+WL+ WL+RE+QAL+QEEDVDIIMHH  GLI+S   R+E K QT+TP
Sbjct: 125 LRYWKSHKYLQPNRWLQSWLRREIQALMQEEDVDIIMHHIHGLIHSSLTRHEQKGQTKTP 184

Query: 193 ELKRKRFSQTILDAAKPFLSARADRFILELELFLASGLNIEAYDSVYLQRLGWNKPIVPS 252
           E K++ F ++I DAA+PFL+AR DRF+ E+ELFLASGLNIEAYD+V +QRLGW+ P V +
Sbjct: 185 EAKQREFQESISDAARPFLAARTDRFVNEVELFLASGLNIEAYDAVCMQRLGWSAPGVTT 244

Query: 253 VANEEDLG-LKSVTPYLYIF---DC 274
              E +L   + V PYLYIF   DC
Sbjct: 245 EPTEGELAEHRPVIPYLYIFYESDC 267

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BLAST of CSPI02G03810 vs. TrEMBL
Match: F6HYY8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0128g00310 PE=4 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 2.6e-95
Identity = 163/267 (61.05%), Postives = 199/267 (74.53%), Query Frame = 1


Query: 13  SMEMETRTSTSDYTNPCPICLGPINQSSYLDKCFHNFCYNCIVQWTKVVSGKRSCTLSSI 72
           S +     S S+  NPCPICLGP+ + SYLD+CFH FCY+CIV+WTKVV+ K     S+I
Sbjct: 14  SKDTSCNDSPSEGPNPCPICLGPVVKDSYLDQCFHKFCYDCIVRWTKVVASKHCRLSSTI 73

Query: 73  KCPLCKTESSSIIHGLDGHNFQRHYVNPDFQDSFILSKAHRYRLQCYYTEPGFLNDIFDV 132
           KCP CKTE+ SI+HG DG +FQ+HY+N DF  SF  SKAH+YRLQCYYTEPG LND F+V
Sbjct: 74  KCPFCKTENFSIVHGYDGSSFQQHYINQDFGKSFSFSKAHKYRLQCYYTEPGILNDKFNV 133

Query: 133 QRYWKLQKYLQANQWLEVWLKRELQALIQEEDVDIIMHHFLGLINSFFRRNEPKYQTETP 192
            RYWK  KY Q NQWL+ WL+RE+QALIQEEDVDII+HH LG+++S  +R E K QT TP
Sbjct: 134 SRYWKFHKYRQPNQWLQAWLRREIQALIQEEDVDIIVHHILGVLDSVIKRTEQKGQTITP 193

Query: 193 ELKRKRFSQTILDAAKPFLSARADRFILELELFLASGLNIEAYDSVYLQRLGWNKPIVPS 252
           E K++ F  ++ DAA+PFL+ R DRF+ E+ELFLASGLNIEAYD VYLQ+LGW  P+  +
Sbjct: 194 ERKQEEFKASVSDAARPFLTGRTDRFVNEVELFLASGLNIEAYDEVYLQQLGWTNPMATT 253

Query: 253 VANEEDLGLKSVTPYLYIFDCDPDDGD 280
              EE  G   V PYLY+FD D D  D
Sbjct: 254 EGVEEQNGHTHVVPYLYLFDYDSDGTD 280

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BLAST of CSPI02G03810 vs. TrEMBL
Match: A0A067F053_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g045854mg PE=4 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 9.9e-95
Identity = 163/259 (62.93%), Postives = 201/259 (77.61%), Query Frame = 1


Query: 22  TSDYTNPCPICLGPINQSSYLDKCFHNFCYNCIVQWTKVVSGKRSCTLSSIKCPLCKTES 81
           +S+  NPCPICLGP+ + SYLDKCFH FCYNCIV WTKVV+ K S  LSS+KCPLCKTE+
Sbjct: 14  SSENPNPCPICLGPVVEDSYLDKCFHKFCYNCIVHWTKVVASKHSSLLSSVKCPLCKTEN 73

Query: 82  SSIIHGLDGHNFQRHYVNPDFQDSFILSKAHRYRLQCYYTEPGFLNDIFDVQRYWKLQKY 141
            SIIHG DG  FQR+Y++  F DSF  SKAHRYRLQ YYTEPG LND+F+V RYWK +KY
Sbjct: 74  VSIIHGYDGTYFQRNYISQIFGDSFFFSKAHRYRLQSYYTEPGILNDVFNVSRYWKSRKY 133

Query: 142 LQANQWLEVWLKRELQALIQEEDVDIIMHHFLGLINSFFRRNEPKYQTETPELKRKRFSQ 201
           LQ+NQWL+ WL+RE+QA++QEEDV+I++HH LG+++SF +RN+ + Q  TPE K + F  
Sbjct: 134 LQSNQWLQSWLRREIQAVMQEEDVEIVVHHILGVVDSFLKRNKQRCQMGTPETKEEDFKA 193

Query: 202 TILDAAKPFLSARADRFILELELFLASGLNIEAYDSVYLQRLGWNKPIVPSVANE-EDLG 261
            + DAA+PFL AR DRF+ E++LFLAS LNIEAYD+VY+QRLGWN P V   + E E  G
Sbjct: 194 LVSDAARPFLMARTDRFVNEMQLFLASALNIEAYDAVYMQRLGWNTPRVTMESGEGETSG 253

Query: 262 LKSVTPYLYIFDCDPDDGD 280
              V PYL+IFD D D  D
Sbjct: 254 QTPVIPYLHIFDEDSDGTD 272

[Close]

BLAST of CSPI02G03810 vs. TrEMBL
Match: V4V3R0_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10002145mg PE=4 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 9.9e-95
Identity = 163/259 (62.93%), Postives = 201/259 (77.61%), Query Frame = 1


Query: 22  TSDYTNPCPICLGPINQSSYLDKCFHNFCYNCIVQWTKVVSGKRSCTLSSIKCPLCKTES 81
           +S+  NPCPICLGP+ + SYLDKCFH FCYNCIV WTKVV+ K S  LSS+KCPLCKTE+
Sbjct: 14  SSENPNPCPICLGPVVEDSYLDKCFHKFCYNCIVHWTKVVASKHSSLLSSVKCPLCKTEN 73

Query: 82  SSIIHGLDGHNFQRHYVNPDFQDSFILSKAHRYRLQCYYTEPGFLNDIFDVQRYWKLQKY 141
            SIIHG DG  FQR+Y++  F DSF  SKAHRYRLQ YYTEPG LND+F+V RYWK +KY
Sbjct: 74  VSIIHGYDGTYFQRNYISQIFGDSFFFSKAHRYRLQSYYTEPGILNDVFNVSRYWKSRKY 133

Query: 142 LQANQWLEVWLKRELQALIQEEDVDIIMHHFLGLINSFFRRNEPKYQTETPELKRKRFSQ 201
           LQ+NQWL+ WL+RE+QA++QEEDV+I++HH LG+++SF +RN+ + Q  TPE K + F  
Sbjct: 134 LQSNQWLQSWLRREIQAVMQEEDVEIVVHHILGVVDSFLKRNKQRCQMGTPETKEEDFKA 193

Query: 202 TILDAAKPFLSARADRFILELELFLASGLNIEAYDSVYLQRLGWNKPIVPSVANE-EDLG 261
            + DAA+PFL AR DRF+ E++LFLAS LNIEAYD+VY+QRLGWN P V   + E E  G
Sbjct: 194 LVSDAARPFLMARTDRFVNEMQLFLASALNIEAYDAVYMQRLGWNTPRVTMESGEGETSG 253

Query: 262 LKSVTPYLYIFDCDPDDGD 280
              V PYL+IFD D D  D
Sbjct: 254 QTPVIPYLHIFDEDSDGTD 272

[Close]

BLAST of CSPI02G03810 vs. TAIR10
Match: AT3G05250.1 (AT3G05250.1 RING/U-box superfamily protein)

HSP 1 Score: 298.9 bits (764), Expect = 3.3e-81
Identity = 144/263 (54.75%), Postives = 190/263 (72.24%), Query Frame = 1


Query: 23  SDYTNPCPICLGPINQSSYLDKCFHNFCYNCIVQWTKVVSGKRSCTLSSIKCPLCKTESS 82
           SD+ +PCPICLG   + SYLD CFH FC+NCI QW KVVS K S   SS+ CPLCKTE+ 
Sbjct: 17  SDF-DPCPICLGQFLRESYLDTCFHKFCFNCIKQWIKVVSSKASKQRSSVTCPLCKTENL 76

Query: 83  SIIHGLDGHNFQRHYVNPDFQDSFILSKAHRYRLQCYYTEPGFLNDIFDVQRYWKLQKYL 142
           SIIH  DG +F+RHY++P+  D F+L+K  RYRLQCYYTE GFL D+FDV R+WKLQK+L
Sbjct: 77  SIIHNYDGCSFERHYIDPNIPDGFVLTKEQRYRLQCYYTESGFLADVFDVARFWKLQKFL 136

Query: 143 QANQWLEVWLKRELQALIQEEDVDIIMHHFLGLINSFFRRNEPKYQTETPELK---RKRF 202
           Q N+ LE WL+RELQAL+QEEDVDI++HH +G++ SF +R + + + ET   +   +++F
Sbjct: 137 QPNRCLEAWLRRELQALMQEEDVDIVLHHLVGVMESFCKRIKQRRKQETRSAETTNQEQF 196

Query: 203 SQTILDAAKPFLSARADRFILELELFLASGLNIEAYDSVYLQRLGWNKPIVPSVANEEDL 262
              + +AA+PF+ AR DRF+ ELELFLA+GLN+EAYD++Y Q L  N       A+EE  
Sbjct: 197 KAVVSEAARPFVMARTDRFVDELELFLAAGLNLEAYDAIYKQGLEGNNRREIGAASEERE 256

Query: 263 GLKS------VTPYLYIFDCDPD 277
            ++       VTPYL+IF+ D D
Sbjct: 257 EVEEHNVRTRVTPYLFIFEEDSD 278

[Close]

BLAST of CSPI02G03810 vs. NCBI nr
Match: gi|778666760|ref|XP_004139186.2| (PREDICTED: uncharacterized protein LOC101211581 isoform X1 [Cucumis sativus])

HSP 1 Score: 581.3 bits (1497), Expect = 9.4e-163
Identity = 278/279 (99.64%), Postives = 279/279 (100.00%), Query Frame = 1


Query: 1   MSGKSMELQSTASMEMETRTSTSDYTNPCPICLGPINQSSYLDKCFHNFCYNCIVQWTKV 60
           MSGKSMELQSTASMEMETRTSTSDYTNPCPICLGPINQSSYLDKCFHNFCYNCIVQWTKV
Sbjct: 1   MSGKSMELQSTASMEMETRTSTSDYTNPCPICLGPINQSSYLDKCFHNFCYNCIVQWTKV 60

Query: 61  VSGKRSCTLSSIKCPLCKTESSSIIHGLDGHNFQRHYVNPDFQDSFILSKAHRYRLQCYY 120
           VSGKRSCTLSSIKCPLCKTESSSIIHGLDGHNFQRHYVNPDFQDSFILSKAHRYRLQCYY
Sbjct: 61  VSGKRSCTLSSIKCPLCKTESSSIIHGLDGHNFQRHYVNPDFQDSFILSKAHRYRLQCYY 120

Query: 121 TEPGFLNDIFDVQRYWKLQKYLQANQWLEVWLKRELQALIQEEDVDIIMHHFLGLINSFF 180
           TEPGFLNDIFDVQRYWKLQKYLQANQWLEVWLKRELQALIQEEDVDIIMHHFLGLINSFF
Sbjct: 121 TEPGFLNDIFDVQRYWKLQKYLQANQWLEVWLKRELQALIQEEDVDIIMHHFLGLINSFF 180

Query: 181 RRNEPKYQTETPELKRKRFSQTILDAAKPFLSARADRFILELELFLASGLNIEAYDSVYL 240
           RRNEP+YQTETPELKRKRFSQTILDAAKPFLSARADRFILELELFLASGLNIEAYDSVYL
Sbjct: 181 RRNEPEYQTETPELKRKRFSQTILDAAKPFLSARADRFILELELFLASGLNIEAYDSVYL 240

Query: 241 QRLGWNKPIVPSVANEEDLGLKSVTPYLYIFDCDPDDGD 280
           QRLGWNKPIVPSVANEEDLGLKSVTPYLYIFDCDPDDGD
Sbjct: 241 QRLGWNKPIVPSVANEEDLGLKSVTPYLYIFDCDPDDGD 279

[Close]

BLAST of CSPI02G03810 vs. NCBI nr
Match: gi|659070388|ref|XP_008454833.1| (PREDICTED: uncharacterized protein LOC103495144 [Cucumis melo])

HSP 1 Score: 548.1 bits (1411), Expect = 8.8e-153
Identity = 263/277 (94.95%), Postives = 271/277 (97.83%), Query Frame = 1


Query: 1   MSGKSMELQSTASMEMETRTSTSDYTNPCPICLGPINQSSYLDKCFHNFCYNCIVQWTKV 60
           MS KSMELQS ASME++T TSTSDYT+PCPICLGPINQSSYLDKCFHNFCYNCIVQWTKV
Sbjct: 1   MSEKSMELQSAASMEIKTSTSTSDYTDPCPICLGPINQSSYLDKCFHNFCYNCIVQWTKV 60

Query: 61  VSGKRSCTLSSIKCPLCKTESSSIIHGLDGHNFQRHYVNPDFQDSFILSKAHRYRLQCYY 120
           VSGKRSC LSSIKCPLCKTESSSIIHGLDGH+FQRHYVNPDFQDSFILSKAH+YRLQCYY
Sbjct: 61  VSGKRSCRLSSIKCPLCKTESSSIIHGLDGHHFQRHYVNPDFQDSFILSKAHKYRLQCYY 120

Query: 121 TEPGFLNDIFDVQRYWKLQKYLQANQWLEVWLKRELQALIQEEDVDIIMHHFLGLINSFF 180
           TEPGFLNDIFDVQRYWKLQKYLQANQWLEVWLKRELQALIQEEDVDIIMHHF+GLINSFF
Sbjct: 121 TEPGFLNDIFDVQRYWKLQKYLQANQWLEVWLKRELQALIQEEDVDIIMHHFVGLINSFF 180

Query: 181 RRNEPKYQTETPELKRKRFSQTILDAAKPFLSARADRFILELELFLASGLNIEAYDSVYL 240
           RRNEPKYQTETPELKRKRFSQTILDAAKPFLSARADRF+LELELFLASGLNIEAYDSVYL
Sbjct: 181 RRNEPKYQTETPELKRKRFSQTILDAAKPFLSARADRFMLELELFLASGLNIEAYDSVYL 240

Query: 241 QRLGWNKPIVPSVANEEDLGLKSVTPYLYIFDCDPDD 278
           QRLGWN+PIV SVANEEDLGLKSVTPYLYIFD DPDD
Sbjct: 241 QRLGWNEPIVLSVANEEDLGLKSVTPYLYIFDNDPDD 277

[Close]

BLAST of CSPI02G03810 vs. NCBI nr
Match: gi|778666763|ref|XP_011648804.1| (PREDICTED: uncharacterized protein LOC101211581 isoform X2 [Cucumis sativus])

HSP 1 Score: 415.6 bits (1067), Expect = 6.8e-113
Identity = 199/200 (99.50%), Postives = 200/200 (100.00%), Query Frame = 1


Query: 80  ESSSIIHGLDGHNFQRHYVNPDFQDSFILSKAHRYRLQCYYTEPGFLNDIFDVQRYWKLQ 139
           ESSSIIHGLDGHNFQRHYVNPDFQDSFILSKAHRYRLQCYYTEPGFLNDIFDVQRYWKLQ
Sbjct: 6   ESSSIIHGLDGHNFQRHYVNPDFQDSFILSKAHRYRLQCYYTEPGFLNDIFDVQRYWKLQ 65

Query: 140 KYLQANQWLEVWLKRELQALIQEEDVDIIMHHFLGLINSFFRRNEPKYQTETPELKRKRF 199
           KYLQANQWLEVWLKRELQALIQEEDVDIIMHHFLGLINSFFRRNEP+YQTETPELKRKRF
Sbjct: 66  KYLQANQWLEVWLKRELQALIQEEDVDIIMHHFLGLINSFFRRNEPEYQTETPELKRKRF 125

Query: 200 SQTILDAAKPFLSARADRFILELELFLASGLNIEAYDSVYLQRLGWNKPIVPSVANEEDL 259
           SQTILDAAKPFLSARADRFILELELFLASGLNIEAYDSVYLQRLGWNKPIVPSVANEEDL
Sbjct: 126 SQTILDAAKPFLSARADRFILELELFLASGLNIEAYDSVYLQRLGWNKPIVPSVANEEDL 185

Query: 260 GLKSVTPYLYIFDCDPDDGD 280
           GLKSVTPYLYIFDCDPDDGD
Sbjct: 186 GLKSVTPYLYIFDCDPDDGD 205

[Close]

BLAST of CSPI02G03810 vs. NCBI nr
Match: gi|595827598|ref|XP_007205719.1| (hypothetical protein PRUPE_ppa009982mg [Prunus persica])

HSP 1 Score: 362.8 bits (930), Expect = 5.2e-97
Identity = 172/265 (64.91%), Postives = 211/265 (79.62%), Query Frame = 1


Query: 13  SMEMETRTSTSDYTNPCPICLGPINQSSYLDKCFHNFCYNCIVQWTKVVSGKRSCTLSSI 72
           ++   T +S+S+ +NPCPICLGPI Q SYLDKCFH FCYNCI++WTKVV+ K+SC  +S+
Sbjct: 5   NVNKNTSSSSSEDSNPCPICLGPIIQDSYLDKCFHKFCYNCILRWTKVVAHKQSCLPTSV 64

Query: 73  KCPLCKTESSSIIHGLDGHNFQRHYVNPDFQDSFILSKAHRYRLQCYYTEPGFLNDIFDV 132
           KCPLCKTE+ SIIHG DG +FQ+HY+N D    FILSK H+YRLQCYYTEPGFLNDIFDV
Sbjct: 65  KCPLCKTENLSIIHGYDGSSFQKHYINQD--SGFILSKDHKYRLQCYYTEPGFLNDIFDV 124

Query: 133 QRYWKLQKYLQANQWLEVWLKRELQALIQEEDVDIIMHHFLGLINSFFRRNEPKYQTETP 192
            RYWK  KYLQ N+WL+ WL+RE+QAL+QEEDVDIIMHH  GLI+S   R+E K QT+TP
Sbjct: 125 LRYWKSHKYLQPNRWLQSWLRREIQALMQEEDVDIIMHHIHGLIHSSLTRHEQKGQTKTP 184

Query: 193 ELKRKRFSQTILDAAKPFLSARADRFILELELFLASGLNIEAYDSVYLQRLGWNKPIVPS 252
           E K++ F ++I DAA+PFL+AR DRF+ E+ELFLASGLNIEAYD+V +QRLGW+ P V +
Sbjct: 185 EAKQREFQESISDAARPFLAARTDRFVNEVELFLASGLNIEAYDAVCMQRLGWSAPGVTT 244

Query: 253 VANEEDLG-LKSVTPYLYIF---DC 274
              E +L   + V PYLYIF   DC
Sbjct: 245 EPTEGELAEHRPVIPYLYIFYESDC 267

[Close]

BLAST of CSPI02G03810 vs. NCBI nr
Match: gi|1009117159|ref|XP_015875165.1| (PREDICTED: uncharacterized protein LOC107411990 [Ziziphus jujuba])

HSP 1 Score: 359.0 bits (920), Expect = 7.6e-96
Identity = 168/261 (64.37%), Postives = 204/261 (78.16%), Query Frame = 1


Query: 20  TSTSDYTNPCPICLGPINQSSYLDKCFHNFCYNCIVQWTKVVSGKRSCTLSSIKCPLCKT 79
           TS+S+ +NPCPICLGPI Q SYLDKC H FCYNCIV WTK+V+ K S  LSS+KCPLCK 
Sbjct: 13  TSSSEDSNPCPICLGPIVQDSYLDKCLHKFCYNCIVHWTKLVTSKHSYKLSSVKCPLCKM 72

Query: 80  ESSSIIHGLDGHNFQRHYVNPDFQDS-FILSKAHRYRLQCYYTEPGFLNDIFDVQRYWKL 139
           E+ S+IHG DG +FQ++Y+N +F++  FIL+KAH+YRLQCYYTEPGFL DIF V RYWK 
Sbjct: 73  ENFSVIHGFDGSSFQQYYINQNFENGGFILTKAHQYRLQCYYTEPGFLGDIFKVSRYWKS 132

Query: 140 QKYLQANQWLEVWLKRELQALIQEEDVDIIMHHFLGLINSFFRRNEPKYQTETPELKRKR 199
            KYLQ N WL+ WLKRE+QALIQEEDV++IMHH LG+INSF RRN+   +++TPE K + 
Sbjct: 133 FKYLQPNCWLQSWLKREIQALIQEEDVEVIMHHILGVINSFMRRNDQHSRSKTPEAKEEE 192

Query: 200 FSQTILDAAKPFLSARADRFILELELFLASGLNIEAYDSVYLQRLGWNKPIVPSVANEED 259
           F   I DAA+PFL+AR +RF+ E+ELFLASGLNIEAYD+VY QRLGW+ P V S  N   
Sbjct: 193 FQALISDAARPFLAARTERFVNEMELFLASGLNIEAYDAVYKQRLGWSAPGVTSETNGGL 252

Query: 260 LGLKSVTPYLYIFDCDPDDGD 280
              + + PYLYIFD D D  D
Sbjct: 253 SEQRPIIPYLYIFDEDSDGTD 273

The following BLAST results are available for this feature:

BLAST of CSPI02G03810 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TOPRS_MOUSE	3.4e-11	32.98	E3 ubiquitin-protein ligase Topors OS=Mus musculus GN=Topors PE=1 SV=1	[more]
TOPRS_HUMAN	7.5e-11	34.83	E3 ubiquitin-protein ligase Topors OS=Homo sapiens GN=TOPORS PE=1 SV=1	[more]

BLAST of CSPI02G03810 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LLN5_CUCSA	6.5e-163	99.64	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021620 PE=4 SV=1	[more]
M5WCB8_PRUPE	3.6e-97	64.91	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009982mg PE=4 SV=1	[more]
F6HYY8_VITVI	2.6e-95	61.05	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0128g00310 PE=4 SV=...	[more]
A0A067F053_CITSI	9.9e-95	62.93	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g045854mg PE=4 SV=1	[more]
V4V3R0_9ROSI	9.9e-95	62.93	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10002145mg PE=4 SV=1	[more]

BLAST of CSPI02G03810 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G05250.1	3.3e-81	54.75	RING/U-box superfamily protein	[more]

BLAST of CSPI02G03810 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778666760\|ref\|XP_004139186.2\|	9.4e-163	99.64	PREDICTED: uncharacterized protein LOC101211581 isoform X1 [Cucumis sativus]	[more]
gi\|659070388\|ref\|XP_008454833.1\|	8.8e-153	94.95	PREDICTED: uncharacterized protein LOC103495144 [Cucumis melo]	[more]
gi\|778666763\|ref\|XP_011648804.1\|	6.8e-113	99.50	PREDICTED: uncharacterized protein LOC101211581 isoform X2 [Cucumis sativus]	[more]
gi\|595827598\|ref\|XP_007205719.1\|	5.2e-97	64.91	hypothetical protein PRUPE_ppa009982mg [Prunus persica]	[more]
gi\|1009117159\|ref\|XP_015875165.1\|	7.6e-96	64.37	PREDICTED: uncharacterized protein LOC107411990 [Ziziphus jujuba]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD
IPR017907	Znf_RING_CS
IPR018957	Znf_C3HC4_RING-type
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD
IPR017907	Znf_RING_CS
IPR018957	Znf_C3HC4_RING-type
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD
IPR017907	Znf_RING_CS
IPR018957	Znf_C3HC4_RING-type

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0046872	metal ion binding
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0046872	metal ion binding
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0046872	metal ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009755	hormone-mediated signaling pathway
biological_process	GO:0008152	metabolic process
biological_process	GO:0007165	signal transduction
biological_process	GO:0008299	isoprenoid biosynthetic process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0006694	steroid biosynthetic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004496	mevalonate kinase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G03810.1	CSPI02G03810.1	mRNA
CSPI02G03810.2	CSPI02G03810.2	mRNA
CSPI02G03810.3	CSPI02G03810.3	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 29..77 score: 7.
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 29..78 score: 11
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 27..83 score: 1.5
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 45..54 scor
IPR018957	Zinc finger, C3HC4 RING-type	PFAM	PF00097	zf-C3HC4	coord: 29..77 score: 2.
None	No IPR available	PANTHER	PTHR22937	RING FINGER CONTAINING PROTEIN	coord: 4..122 score: 7.6
None	No IPR available	PANTHER	PTHR22937:SF39	E3 UBIQUITIN-PROTEIN LIGASE TOPORS-RELATED	coord: 4..122 score: 7.6
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 25..87 score: 4.78

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI02G03810	Cla019879	Watermelon (97103) v1	cpiwmB149
CSPI02G03810	Csa2G021620	Cucumber (Chinese Long) v2	cpicuB065
CSPI02G03810	MELO3C004279	Melon (DHL92) v3.5.1	cpimeB139
CSPI02G03810	ClCG02G015910	Watermelon (Charleston Gray)	cpiwcgB128
CSPI02G03810	Lsi11G005300	Bottle gourd (USVL1VR-Ls)	cpilsiB096
CSPI02G03810	MELO3C004279.2	Melon (DHL92) v3.6.1	cpimedB132
CSPI02G03810	CsaV3_2G005130	Cucumber (Chinese Long) v3	cpicucB080
CSPI02G03810	Cla97C02G041680	Watermelon (97103) v2	cpiwmbB120
CSPI02G03810	Bhi05G001654	Wax gourd	cpiwgoB149
CSPI02G03810	Cucsa.091610	Cucumber (Gy14) v1	cgycpiB097
CSPI02G03810	CmaCh05G011480	Cucurbita maxima (Rimu)	cmacpiB800
CSPI02G03810	CmoCh05G011590	Cucurbita moschata (Rifu)	cmocpiB787
CSPI02G03810	Cp4.1LG11g09450	Cucurbita pepo (Zucchini)	cpecpiB100
CSPI02G03810	CsGy2G003900	Cucumber (Gy14) v2	cgybcpiB056
CSPI02G03810	Carg12894	Silver-seed gourd	carcpiB0938

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI02G03810	Cucurbita maxima (Rimu)	cmacpiB174
CSPI02G03810	Cucurbita moschata (Rifu)	cmocpiB159
CSPI02G03810	Cucurbita pepo (Zucchini)	cpecpiB821
CSPI02G03810	Silver-seed gourd	carcpiB0751