Carg12293 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg12293
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Iron-sulfur cluster assembly
Location	Cucurbita_argyrosperma_scaffold_074 : 121067 .. 124004 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATGAAGAAAAAGAAGAGACGGTTGCCGCGAATCTATCTTCCTCCTATGCTGTGTTCATCCCTGATTCGAGCTGAAGCCTTCTCTCTGTAATCTCCATCCTTCTTGGTAGGGTTGAATCGCTGCGTTCTTCCTCCGCTTCTCGATTCCTCTTTGGGTTTAATCGAGCGTGGCGGATGTGGGGAAGAAAATGAACTACGGGACCCCTAAACTTCCTTCAAGGGTGGCGATTTTTCCGATAAAGTTATCGATGCTAACGATTAACATTGGCGGAGTTGTGGATTGCTTTTGTGCCTCTGTTTCAAGCGTCTCCAACATGTAAGAACCTTCTATTCACTTTTTTTTTTTTTTTTTTCCTGCTTGTTTTTCCTTCTTTTGCTTACGATTTCCATTTTCCCGAGTGTTCTTTGACGGAAAAGGATGAATCCACAAAGAAGAATGAGTTTTCTGGCTGGTTATTAGCAGTAGCTTTCCCTCCAATTTAAATTGAGAATTTCTGATTACTTGAGTTTGTTGTAGAATTAGCTTTTCTAACAAAACAATTGCTCAGAAACTCGATTCATGCCTCTAAGAAAAACCATTTATTTAATGAAAGAATACTAGGAGCTTACAAGAGGAAAGCCCCTCACAAAAGAGCTCAACTGAAATAATACCTATGGTATAGTTACAAAGAAGTTCTAAAACTAACCACTTGTAACCGCCCAAAGCCACCGCTAACAGATATTGTTCGCTTTGGCCATTACGTATAGCCGTCAACCTCACGGTTTTAAAACACGTCTACTAGAAAGAGGTTTCCACACCCTTGTAAGGAATGTTTCGTTCCACTCTCCAACCGATGTGGGATCTCACAATCCACCCCTTTCGGGGTCCAGCGTTCTCGCTGGCTCACCGCCCCCCTTTGGAGTTCAACCTTCTCGCAATCCACCTTTTTGGAGGTTTCCACACACGTCTACTATAGGTTGACTTAGTGGTGAATAAGGGCTATGTAAATAATAGAGGGATAGGCCAAGCCATGATGACCTCCTACTTAGGATTTAATACCTCACGAGTTATGTTGGCATCTAGTAGGTCAAGCAATTATCCTAGGAGAATAGTTGAGGTGCGTGCAAGTCAGCCTCAACACTCATGAACATAAAAATAAAATGTCGTGGAAACTAGATATGGAATCTATCCGAAACTAGCTTTCATCACTATAGCTATAAGTGTGTTTGGAGTGGGTCAATTCAAAATGTTGCATTTCCATCTTTGTTTCAATTGACTCGGATAGAAGCATTGTCCCCATTAGTCATGTTAATACCCTTAGCACATTGCGTTTTCCGCAGATAAGGATCAAATTTAAAGAAGATAGAGTGAAGAGATGGTCCCCAGATTGCTAAGTCAGCGGCTAGCTCCATACTTGACAGCACGTATCCGAGAGAACCAGAGGCTTCTTAGCTCCTCTGTTTCAGCTCTTCAAGAAGCCTCTTCTTTGTTGACATCTCCAGCTCAATCTCCATCTCCACACACCGATACCATTCACATGACAGATAATTGCATCCGGGTACTCCAACATATTATGTAATCCTTTTTTGTGCATGTACCAAATATGCTTAGGCAATTTTTATCCATTTCACTGTCATAATTTGTTTTAGTTCTTGGTTTCTGGACTATCCTCATTTTGTGCACCATTTCACTGTCACTGTCATAATTTTTGTGCACTATTTTAGTTCTTGGTACTCCACATAAGACGAATTGAAACATCTTTAGATAATTTTGACAGCATTTCTTCTCATCTTCTTTGAAGTATATTGTATGATTAATGTCTGTTGGTCCATATCACTTGTAACCTTGATAATGAACTCTTCATGATAAGATGTATGATGTACTTTCTTTTACCAAAGGGCTAAATTGGTCGATACTCGCTTTATATCATGGATAAAATATATATTAATACCATTGTCCTGCATCTGCATCTGCATCTGCATCTGCATCTTTTAAAAGGGTTCATTCAATCTTCTTTTCTACTTAGTTTTCCTTTTCTTCTGCGTGTATTGTAAGTGTTGAATAATTACTTCTTATGAAGAACGATCTTGATTTACTTTCTGCTGAGTCTTGACATGCCTTGCAGAGAATGAAAGAATTGCAAGAACCCATGGAAGAAAAGATGCTTCGCTTGAGTGTAGAAACTGGGGGGTGTTCTGGATTTCAATATGTTTTCAATTTGGATGACCAAACCAATCCAGATGACAGGTTCATTGAAATACTAGCTCATACTTGCTGCTTATTTTGGGTTTTTCACCTAAAAATTGTCATTGATGTTTCCTTTTACATTTTTAAAAGTTTACCCTTCTCTCTTCTTTCTTGTTTGCAGGGTGTGTGAGAAGGAGGGAGTTAAATTGGTAGTTGATAATATCTCATATGATTTTGTGAAAGGAGCAACAGTTGATTATGTTGAAGAGCTGATTCGTTGCGCTTTCGTTGTGAGTAAACCTTTATCAGTATTGTTGGCTCGCTCCGTTTTTGCTTCCATGCGTGACTTGTTCTATTTCAGTTCTTATACTCTTAAACGTCCAAATTTAGTTCAATAAATCTAAAACGTTGTCCATGAAGTTCTTTTATGGTAACTTTTTTACAATAACTAAAGTGGAATGATACCCAAAGTACAGGAACCAAAATGATATTTTAACTTTACTATATTTTATATTTGTGTGTTCTCTAACAAATCGAGCTTATAAAACATCTGCAAGCGAAAGATTTGGGAGTGAAGTACCCTTAAGTAGTAGAATTCTCCTCATTTTTCTCCATGTGTTCTATGCATTCGTATCTTTCAATAAAGCAAGTGCTGATGCAGCACAAGTTGATTTCTTGTACCATTGTCTGTGTGCAGGTGACCACAAATCCAAGTGCTGTTGGGGGCTGTAGTTGTAAGAGTTCTTTCATGGTGAAACAATGATCACCTC

mRNA sequence

AAATGAAGAAAAAGAAGAGACGGTTGCCGCGAATCTATCTTCCTCCTATGCTGTGTTCATCCCTGATTCGAGCTGAAGCCTTCTCTCTGTAATCTCCATCCTTCTTGGTAGGGTTGAATCGCTGCGTTCTTCCTCCGCTTCTCGATTCCTCTTTGGGTTTAATCGAGCGTGGCGGATGTGGGGAAGAAAATGAACTACGGGACCCCTAAACTTCCTTCAAGGGTGGCGATTTTTCCGATAAAGTTATCGATGCTAACGATTAACATTGGCGGAGTTGTGGATTGCTTTTGTGCCTCTGTTTCAAGCGTCTCCAACATATAAGGATCAAATTTAAAGAAGATAGAGTGAAGAGATGGTCCCCAGATTGCTAAGTCAGCGGCTAGCTCCATACTTGACAGCACGTATCCGAGAGAACCAGAGGCTTCTTAGCTCCTCTGTTTCAGCTCTTCAAGAAGCCTCTTCTTTGTTGACATCTCCAGCTCAATCTCCATCTCCACACACCGATACCATTCACATGACAGATAATTGCATCCGGAGAATGAAAGAATTGCAAGAACCCATGGAAGAAAAGATGCTTCGCTTGAGTGTAGAAACTGGGGGGTGTTCTGGATTTCAATATGTTTTCAATTTGGATGACCAAACCAATCCAGATGACAGGGTGTGTGAGAAGGAGGGAGTTAAATTGGTAGTTGATAATATCTCATATGATTTTGTGAAAGGAGCAACAGTTGATTATGTTGAAGAGCTGATTCGTTGCGCTTTCGTTGTGACCACAAATCCAAGTGCTGTTGGGGGCTGTAGTTGTAAGAGTTCTTTCATGGTGAAACAATGATCACCTC

Coding sequence (CDS)

ATGGTCCCCAGATTGCTAAGTCAGCGGCTAGCTCCATACTTGACAGCACGTATCCGAGAGAACCAGAGGCTTCTTAGCTCCTCTGTTTCAGCTCTTCAAGAAGCCTCTTCTTTGTTGACATCTCCAGCTCAATCTCCATCTCCACACACCGATACCATTCACATGACAGATAATTGCATCCGGAGAATGAAAGAATTGCAAGAACCCATGGAAGAAAAGATGCTTCGCTTGAGTGTAGAAACTGGGGGGTGTTCTGGATTTCAATATGTTTTCAATTTGGATGACCAAACCAATCCAGATGACAGGGTGTGTGAGAAGGAGGGAGTTAAATTGGTAGTTGATAATATCTCATATGATTTTGTGAAAGGAGCAACAGTTGATTATGTTGAAGAGCTGATTCGTTGCGCTTTCGTTGTGACCACAAATCCAAGTGCTGTTGGGGGCTGTAGTTGTAAGAGTTCTTTCATGGTGAAACAATGA

Protein sequence

MVPRLLSQRLAPYLTARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCIRRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDFVKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ

Homology

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BLAST of Carg12293 vs. NCBI nr
Match: XP_022948821.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita moschata])

HSP 1 Score: 314.7 bits (805), Expect = 1.8e-82
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 1   MVPRLLSQRLAPYLTARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCI 60
           MVPRLLSQRLAPYLTARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCI
Sbjct: 1   MVPRLLSQRLAPYLTARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCI 60

Query: 61  RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 120
           RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF
Sbjct: 61  RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 120

Query: 121 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 160
           VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 159

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BLAST of Carg12293 vs. NCBI nr
Match: XP_022998904.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita maxima])

HSP 1 Score: 312.8 bits (800), Expect = 6.9e-82
Identity = 158/159 (99.37%), Postives = 158/159 (99.37%), Query Frame = 0


Query: 1   MVPRLLSQRLAPYLTARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCI 60
           MVPRLLSQRLAPYL ARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCI
Sbjct: 1   MVPRLLSQRLAPYLAARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCI 60

Query: 61  RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 120
           RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF
Sbjct: 61  RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 120

Query: 121 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 160
           VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of Carg12293 vs. NCBI nr
Match: XP_023523533.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 305.8 bits (782), Expect = 8.4e-80
Identity = 155/159 (97.48%), Postives = 156/159 (98.11%), Query Frame = 0


Query: 1   MVPRLLSQRLAPYLTARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCI 60
           MVPRLLSQRLAPYL ARIRENQRLLSSSVSALQEASSLLTSP+QSPSP  DTIHMTDNCI
Sbjct: 1   MVPRLLSQRLAPYLAARIRENQRLLSSSVSALQEASSLLTSPSQSPSPDIDTIHMTDNCI 60

Query: 61  RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 120
           RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF
Sbjct: 61  RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 120

Query: 121 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 160
           VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 159

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BLAST of Carg12293 vs. NCBI nr
Match: XP_022152826.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial [Momordica charantia])

HSP 1 Score: 266.9 bits (681), Expect = 4.3e-68
Identity = 137/159 (86.16%), Postives = 144/159 (90.57%), Query Frame = 0


Query: 1   MVPRLLSQRLAPYLTARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCI 60
           MVPRLL QRL P+  ARIRENQRLLSSSVSA QE  SLLTSP+ S S   DTIHMTDNCI
Sbjct: 1   MVPRLLIQRLTPHFIARIRENQRLLSSSVSAHQEVPSLLTSPSPSSSVLIDTIHMTDNCI 60

Query: 61  RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 120
           ++MKELQEPMEEKMLRLSVETGGCSGFQYVFNLDD+TNPDDRV EK+GVKLVVDNISYDF
Sbjct: 61  QKMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDKTNPDDRVYEKDGVKLVVDNISYDF 120

Query: 121 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 160
           VKGAT+DY EELIR AFVVTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATIDYAEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 159

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BLAST of Carg12293 vs. NCBI nr
Match: XP_004147288.1 (PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X1 [Cucumis sativus])

HSP 1 Score: 263.5 bits (672), Expect = 4.8e-67
Identity = 136/159 (85.53%), Postives = 142/159 (89.31%), Query Frame = 0


Query: 1   MVPRLLSQRLAPYLTARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCI 60
           MVPR L QRL PYL A IR+NQRLLSSSVSA QEASS L     +PS   DTIHM+DNCI
Sbjct: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLI----TPSAGIDTIHMSDNCI 60

Query: 61  RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 120
           RRMKELQEPMEEKMLRLSVE GGCSGFQYVFNLDD+TNPDDRVCEKEGVKLVVDNISYDF
Sbjct: 61  RRMKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDF 120

Query: 121 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 160
           VKGAT+DYVEELIR AF+VTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 155

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BLAST of Carg12293 vs. TAIR10
Match: AT5G03905.1 (Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 194.1 bits (492), Expect = 6.4e-50
Identity = 107/158 (67.72%), Postives = 120/158 (75.95%), Query Frame = 0


Query: 4   RLLSQRLAPYLTARIRENQRLLS-SSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCIRR 63
           R L +R+APYL  RIREN RLL+ SS SA++                 D +H++DNCIRR
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIK--XXXXXXXXXXXXXXXDVVHLSDNCIRR 62

Query: 64  MKELQ--EPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 123
           MKELQ  EP E+KMLRL VETGGCSGFQY F LD++TNPDDRV EK GVKLVVDN+SYD 
Sbjct: 63  MKELQSSEP-EKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYDL 122

Query: 124 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVK 159
           VKGAT+DY EELIR AFVV  NPSAVGGCSCKSSFMVK
Sbjct: 123 VKGATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMVK 157

[Close]

BLAST of Carg12293 vs. TAIR10
Match: AT2G36260.1 (Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 68.2 bits (165), Expect = 5.3e-12
Identity = 36/101 (35.64%), Postives = 58/101 (57.43%), Query Frame = 0


Query: 55  MTDNCIRRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVD 114
           ++D    R+++L +  ++  LRL+VE  GC+G  YV N   +    D V E++GVK++VD
Sbjct: 8   LSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKGVKILVD 67

Query: 115 NISYDFVKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSF 156
             +   V G  +D+V++ +R  FV   NP+A   C C  SF
Sbjct: 68  PKAVMHVIGTEMDFVDDKLRSEFVF-VNPNAT-KCGCGESF 106

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BLAST of Carg12293 vs. Swiss-Prot
Match: sp|Q8LCY2|ISAM2_ARATH (Iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g03905 PE=1 SV=2)

HSP 1 Score: 194.1 bits (492), Expect = 1.2e-48
Identity = 107/158 (67.72%), Postives = 120/158 (75.95%), Query Frame = 0


Query: 4   RLLSQRLAPYLTARIRENQRLLS-SSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCIRR 63
           R L +R+APYL  RIREN RLL+ SS SA++                 D +H++DNCIRR
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIK--XXXXXXXXXXXXXXXDVVHLSDNCIRR 62

Query: 64  MKELQ--EPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 123
           MKELQ  EP E+KMLRL VETGGCSGFQY F LD++TNPDDRV EK GVKLVVDN+SYD 
Sbjct: 63  MKELQSSEP-EKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYDL 122

Query: 124 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVK 159
           VKGAT+DY EELIR AFVV  NPSAVGGCSCKSSFMVK
Sbjct: 123 VKGATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMVK 157

[Close]

BLAST of Carg12293 vs. Swiss-Prot
Match: sp|Q2TBG7|ISCA2_BOVIN (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Bos taurus OX=9913 GN=ISCA2 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 8.7e-28
Identity = 73/141 (51.77%), Postives = 90/141 (63.83%), Query Frame = 0


Query: 20  ENQRLLSSS--VSALQEASSLLTSPAQSPSPHTDTIHMTDNCIRRMKELQEPMEEKMLRL 79
           +  RLL++S    AL+E S        SP      IH+TD+C++R+ E+ E  E   LRL
Sbjct: 19  QRSRLLAASRGPRALREVS------FSSPEAGEGQIHLTDSCVQRLLEITEGSE--FLRL 78

Query: 80  SVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDFVKGATVDYVEELIRCAF 139
            VE GGCSGFQY F+LD   NPDDRV EK G ++VVD+ S  FVKGA VD+ +ELIR +F
Sbjct: 79  EVEGGGCSGFQYKFSLDTVINPDDRVFEKGGARVVVDSDSLAFVKGAHVDFSQELIRSSF 138

Query: 140 VVTTNPSAVGGCSCKSSFMVK 159
            V  NP A  GCSC SSF VK
Sbjct: 139 QVLNNPQAQQGCSCGSSFSVK 151

[Close]

BLAST of Carg12293 vs. Swiss-Prot
Match: sp|Q86U28|ISCA2_HUMAN (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Homo sapiens OX=9606 GN=ISCA2 PE=1 SV=2)

HSP 1 Score: 122.1 bits (305), Expect = 5.6e-27
Identity = 71/138 (51.45%), Postives = 90/138 (65.22%), Query Frame = 0


Query: 23  RLLSSSV--SALQEASSLLTSPAQSPSPHTDTIHMTDNCIRRMKELQEPMEEKMLRLSVE 82
           RLL++S+   A +EASS       SP      I +TD+C++R+ E+ E  E   LRL VE
Sbjct: 24  RLLTASLGPQARREASS------SSPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVE 83

Query: 83  TGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDFVKGATVDYVEELIRCAFVVT 142
            GGCSGFQY F+LD   NPDDRV E+ G ++VVD+ S  FVKGA VD+ +ELIR +F V 
Sbjct: 84  GGGCSGFQYKFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVL 143

Query: 143 TNPSAVGGCSCKSSFMVK 159
            NP A  GCSC SSF +K
Sbjct: 144 NNPQAQQGCSCGSSFSIK 153

[Close]

BLAST of Carg12293 vs. Swiss-Prot
Match: sp|Q5R788|ISCA2_PONAB (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Pongo abelii OX=9601 GN=ISCA2 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 5.6e-27
Identity = 71/138 (51.45%), Postives = 90/138 (65.22%), Query Frame = 0


Query: 23  RLLSSSV--SALQEASSLLTSPAQSPSPHTDTIHMTDNCIRRMKELQEPMEEKMLRLSVE 82
           RLL++S+   A +EASS       SP      I +TD+C++R+ E+ E  E   LRL VE
Sbjct: 24  RLLAASLGPQARREASS------SSPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVE 83

Query: 83  TGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDFVKGATVDYVEELIRCAFVVT 142
            GGCSGFQY F+LD   NPDDRV E+ G ++VVD+ S  FVKGA VD+ +ELIR +F V 
Sbjct: 84  GGGCSGFQYKFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVL 143

Query: 143 TNPSAVGGCSCKSSFMVK 159
            NP A  GCSC SSF +K
Sbjct: 144 NNPQAQQGCSCGSSFSIK 153

[Close]

BLAST of Carg12293 vs. Swiss-Prot
Match: sp|Q9DCB8|ISCA2_MOUSE (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Mus musculus OX=10090 GN=Isca2 PE=1 SV=2)

HSP 1 Score: 119.4 bits (298), Expect = 3.6e-26
Identity = 62/113 (54.87%), Postives = 76/113 (67.26%), Query Frame = 0


Query: 46  PSPHTDTIHMTDNCIRRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCE 105
           P      I +TD+C++R+ E+ E  E   LRL VE GGCSGFQY F+LD   NPDDRV E
Sbjct: 43  PEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGGGCSGFQYKFSLDTVINPDDRVFE 102

Query: 106 KEGVKLVVDNISYDFVKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVK 159
           + G ++VVD+ S  FVKGA VD+ +ELIR +F V  NP A  GCSC SSF VK
Sbjct: 103 QGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQAQQGCSCGSSFSVK 153

[Close]

BLAST of Carg12293 vs. TrEMBL
Match: tr|A0A0A0LXX8|A0A0A0LXX8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G496290 PE=4 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 4.3e-64
Identity = 131/159 (82.39%), Postives = 140/159 (88.05%), Query Frame = 0


Query: 1   MVPRLLSQRLAPYLTARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCI 60
           M PR L QR+ PYL ARI+ENQRLLSSS SA QEA  L  SP+ SPS   DTIHMTD+CI
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALPL--SPSPSPSAPVDTIHMTDSCI 60

Query: 61  RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 120
           RRMKELQ+P EEKMLRLSVETGGCSGFQYVFNLD +TNPDDR+ EKEGVKLVVDNISYDF
Sbjct: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDF 120

Query: 121 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 160
           VKGAT+DYVEELIR AFVV+TNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 157

[Close]

BLAST of Carg12293 vs. TrEMBL
Match: tr|A0A1S3C5P3|A0A1S3C5P3_CUCME (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103497193 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.3e-60
Identity = 124/159 (77.99%), Postives = 133/159 (83.65%), Query Frame = 0


Query: 1   MVPRLLSQRLAPYLTARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCI 60
           M PR L QR+ PYL ARI+ENQRLLSSS +A QEAS              DTIHMTD+CI
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSATAYQEASXXXXXXXXXXXXPVDTIHMTDSCI 60

Query: 61  RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 120
           RRMKELQ+P EEKMLRLSVETGGCSGFQYVFNLD +T PDDR+ EKEGVKLVVDNISYDF
Sbjct: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTTPDDRIYEKEGVKLVVDNISYDF 120

Query: 121 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 160
           VKGAT+DYVEELIR AFVV+TNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of Carg12293 vs. TrEMBL
Match: tr|A0A1R3HDF4|A0A1R3HDF4_9ROSI (FeS cluster biogenesis OS=Corchorus olitorius OX=93759 GN=COLO4_29735 PE=4 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.5e-56
Identity = 122/160 (76.25%), Postives = 136/160 (85.00%), Query Frame = 0


Query: 4   RLLSQRLAPYLTARIRENQRLL-SSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCIRR 63
           R L QR++P+  ARI++N +LL SSS SAL EASS     A SP+P  D IH+TDNC++R
Sbjct: 3   RSLIQRISPFFIARIKQNHKLLSSSSSSALHEASS---ESAPSPAPSLDAIHLTDNCVKR 62

Query: 64  MKELQ---EPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYD 123
           MKELQ   E  EEKMLRLSVETGGCSGFQYVFNLDD+TNP+DRV EKEGVKLVVD+ISYD
Sbjct: 63  MKELQSSEESSEEKMLRLSVETGGCSGFQYVFNLDDKTNPEDRVFEKEGVKLVVDDISYD 122

Query: 124 FVKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 160
           FVKGATVD+VEELIR AFVVTTNPSAVGGCSCKSSFMVKQ
Sbjct: 123 FVKGATVDFVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of Carg12293 vs. TrEMBL
Match: tr|A0A1R3II09|A0A1R3II09_COCAP (FeS cluster biogenesis OS=Corchorus capsularis OX=210143 GN=CCACVL1_12023 PE=4 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 2.5e-56
Identity = 121/160 (75.62%), Postives = 136/160 (85.00%), Query Frame = 0


Query: 4   RLLSQRLAPYLTARIRENQRLL-SSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCIRR 63
           R L QR++P+  ARI++N +LL SSS SAL EASS     A SP+P  D IH+TDNC++R
Sbjct: 3   RSLIQRISPFFIARIKQNHKLLSSSSSSALHEASS---ESAPSPAPSLDAIHLTDNCVKR 62

Query: 64  MKELQ---EPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYD 123
           MKELQ   E  EEKMLRLSVETGGCSGFQYVFNLDD+TNP+DRV E+EGVKLVVD+ISYD
Sbjct: 63  MKELQSSEESSEEKMLRLSVETGGCSGFQYVFNLDDKTNPEDRVFEREGVKLVVDDISYD 122

Query: 124 FVKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 160
           FVKGATVDYVEELIR AF+VTTNPSAVGGCSCKSSFMVKQ
Sbjct: 123 FVKGATVDYVEELIRSAFLVTTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of Carg12293 vs. TrEMBL
Match: tr|A0A0B0MTF5|A0A0B0MTF5_GOSAR (Uncharacterized protein OS=Gossypium arboreum OX=29729 GN=F383_28491 PE=4 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.1e-54
Identity = 114/159 (71.70%), Postives = 135/159 (84.91%), Query Frame = 0


Query: 4   RLLSQRLAPYLTARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCIRRM 63
           R L QR++P+  ARI++N +LLSSS S+   +S+L  + ++SPSP  D +HMTDNCI+RM
Sbjct: 11  RSLIQRISPFFIARIKQNHKLLSSSFSS--SSSALHEASSESPSPSLDAVHMTDNCIKRM 70

Query: 64  KELQ---EPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 123
           KELQ   +  + KMLRLSVETGGCSGFQYVF+LDD+TNPDDRV  +EGVKLVVD+ISYDF
Sbjct: 71  KELQASEQSSDHKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVFVREGVKLVVDDISYDF 130

Query: 124 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 160
           VKGAT+DYVEELIR AFVVTTNPSAVGGCSCKSSFMVKQ
Sbjct: 131 VKGATIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 167

The following BLAST results are available for this feature:

BLAST of Carg12293 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022948821.1	1.8e-82	100.00	iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita moschata]	[more]
XP_022998904.1	6.9e-82	99.37	iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita maxima]	[more]
XP_023523533.1	8.4e-80	97.48	iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita pepo subsp. p...	[more]
XP_022152826.1	4.3e-68	86.16	iron-sulfur assembly protein IscA-like 2, mitochondrial [Momordica charantia]	[more]
XP_004147288.1	4.8e-67	85.53	PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X1 [C...	[more]

BLAST of Carg12293 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G03905.1	6.4e-50	67.72	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G36260.1	5.3e-12	35.64	Iron-sulphur cluster biosynthesis family protein	[more]

BLAST of Carg12293 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q8LCY2\|ISAM2_ARATH	1.2e-48	67.72	Iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Arabidopsis thaliana ...	[more]
sp\|Q2TBG7\|ISCA2_BOVIN	8.7e-28	51.77	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Bos taurus OX=9913 GN=I...	[more]
sp\|Q86U28\|ISCA2_HUMAN	5.6e-27	51.45	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Homo sapiens OX=9606 GN...	[more]
sp\|Q5R788\|ISCA2_PONAB	5.6e-27	51.45	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Pongo abelii OX=9601 GN...	[more]
sp\|Q9DCB8\|ISCA2_MOUSE	3.6e-26	54.87	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Mus musculus OX=10090 G...	[more]

BLAST of Carg12293 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LXX8\|A0A0A0LXX8_CUCSA	4.3e-64	82.39	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G496290 PE=4 SV=1	[more]
tr\|A0A1S3C5P3\|A0A1S3C5P3_CUCME	1.3e-60	77.99	iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucumis melo OX=3656 ...	[more]
tr\|A0A1R3HDF4\|A0A1R3HDF4_9ROSI	1.5e-56	76.25	FeS cluster biogenesis OS=Corchorus olitorius OX=93759 GN=COLO4_29735 PE=4 SV=1	[more]
tr\|A0A1R3II09\|A0A1R3II09_COCAP	2.5e-56	75.63	FeS cluster biogenesis OS=Corchorus capsularis OX=210143 GN=CCACVL1_12023 PE=4 S...	[more]
tr\|A0A0B0MTF5\|A0A0B0MTF5_GOSAR	1.1e-54	71.70	Uncharacterized protein OS=Gossypium arboreum OX=29729 GN=F383_28491 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005198	structural molecule activity
GO:0051536	iron-sulfur cluster binding

Vocabulary: **Biological Process**
Term	Definition
GO:0097428	protein maturation by iron-sulfur cluster transfer

Vocabulary: **INTERPRO**
Term	Definition
IPR016092	FeS_cluster_insertion
IPR000361	FeS_biogenesis
IPR035903	HesB-like_dom_sf

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0097428	protein maturation by iron-sulfur cluster transfer
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0005198	structural molecule activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12293-RA	Carg12293-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035903	HesB-like domain superfamily	GENE3D	G3DSA:2.60.300.12		coord: 52..159 e-value: 5.0E-32 score: 112.1
IPR035903	HesB-like domain superfamily	SUPERFAMILY	SSF89360	HesB-like domain	coord: 52..145
IPR000361	FeS cluster biogenesis	PFAM	PF01521	Fe-S_biosyn	coord: 53..152 e-value: 1.7E-14 score: 53.9
IPR016092	FeS cluster insertion protein	TIGRFAM	TIGR00049	TIGR00049	coord: 53..157 e-value: 5.9E-33 score: 111.2
None	No IPR available	PANTHER	PTHR43011	FAMILY NOT NAMED	coord: 17..159

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg12293	Cla005089	Watermelon (97103) v1	carwmB0440
Carg12293	Csa1G108280	Cucumber (Chinese Long) v2	carcuB0472
Carg12293	ClCG03G002740	Watermelon (Charleston Gray)	carwcgB0436
Carg12293	CSPI01G13300	Wild cucumber (PI 183967)	carcpiB0477
Carg12293	Cucsa.292560	Cucumber (Gy14) v1	carcgyB0514
Carg12293	CmaCh01G005170	Cucurbita maxima (Rimu)	carcmaB0613
Carg12293	CmoCh01G005490	Cucurbita moschata (Rifu)	carcmoB0596
Carg12293	Cp4.1LG02g12660	Cucurbita pepo (Zucchini)	carcpeB0622
Carg12293	CsGy1G013410	Cucumber (Gy14) v2	carcgybB0449
Carg12293	CsaV3_1G013480	Cucumber (Chinese Long) v3	carcucB0474
Carg12293	Cla97C03G053770	Watermelon (97103) v2	carwmbB0467
Carg12293	Bhi08G000209	Wax gourd	carwgoB0532

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg12293	Silver-seed gourd	carcarB448
Carg12293	Silver-seed gourd	carcarB450
Carg12293	Cucumber (Gy14) v2	carcgybB0451
Carg12293	Cucurbita maxima (Rimu)	carcmaB0611
Carg12293	Cucurbita moschata (Rifu)	carcmoB0594
Carg12293	Cucurbita pepo (Zucchini)	carcpeB0620
Carg12293	Wild cucumber (PI 183967)	carcpiB0479
Carg12293	Cucumber (Chinese Long) v2	carcuB0474
Carg12293	Cucumber (Chinese Long) v3	carcucB0476
Carg12293	Bottle gourd (USVL1VR-Ls)	carlsiB404
Carg12293	Melon (DHL92) v3.5.1	carmeB0449
Carg12293	Melon (DHL92) v3.6.1	carmedB0452
Carg12293	Watermelon (Charleston Gray)	carwcgB0432
Carg12293	Watermelon (97103) v1	carwmB0439
Carg12293	Wax gourd	carwgoB0528