Lsi08G008230 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi08G008230
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionEthylene-responsive transcription factor 2
Locationchr08 : 16615372 .. 16616010 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAATAATCCCGATCTCCTCCAAACTTCCGCCGCCGGCGACACCAAGTACAGAGGAGTACGGCGGCGCAAATGGGGGAAATGGGTTGCTGAAATCCGTCTCCCTAATAGCCGCGACCGTATCTGGCTTGGTTCTTACGACTCACCAGAGAAAGCTGCCCGAGCCTTTGACGCTGCCCTTTTCTGTCTCCGTGGACCTCATGCCAAGTTTAACTTCCCTGACCAACCACTGCCCGACATTCTCAACGCTCATTCTCTCACTGCCCATCAGATCCAAGAATTGGCTGCGAAGTTCGCTAATGAATATCATCAAAACGACGACGTTGTGGTTGAGGGTATTGTAGTACCTCCAAATCCACCCACTGAGGACAATTGTGGAAATTCACCTTCCTCCTCCTCCAACAATATTAATAATTATAACAATATGGATTGGTCTTTCCTTGATGCCATTCATGAAGACCTTCCTCATTCTTCTTCTTCTTCCTCCAATTTCTTTCCTCTTTACAATGACCATTTTGATAATATCTTAACCAATGATTTTTATCAATCCACCAACAACAACGACCTTTACGACGTCGTTTCGGGTGATGACATTGACGACACTTTCTCCAATCAACCCTTTCTATGGAATTTTTAA

mRNA sequence

ATGAACAATAATCCCGATCTCCTCCAAACTTCCGCCGCCGGCGACACCAAGTACAGAGGAGTACGGCGGCGCAAATGGGGGAAATGGGTTGCTGAAATCCGTCTCCCTAATAGCCGCGACCGTATCTGGCTTGGTTCTTACGACTCACCAGAGAAAGCTGCCCGAGCCTTTGACGCTGCCCTTTTCTGTCTCCGTGGACCTCATGCCAAGTTTAACTTCCCTGACCAACCACTGCCCGACATTCTCAACGCTCATTCTCTCACTGCCCATCAGATCCAAGAATTGGCTGCGAAGTTCGCTAATGAATATCATCAAAACGACGACGTTGTGGTTGAGGGTATTGTAGTACCTCCAAATCCACCCACTGAGGACAATTGTGGAAATTCACCTTCCTCCTCCTCCAACAATATTAATAATTATAACAATATGGATTGGTCTTTCCTTGATGCCATTCATGAAGACCTTCCTCATTCTTCTTCTTCTTCCTCCAATTTCTTTCCTCTTTACAATGACCATTTTGATAATATCTTAACCAATGATTTTTATCAATCCACCAACAACAACGACCTTTACGACGTCGTTTCGGGTGATGACATTGACGACACTTTCTCCAATCAACCCTTTCTATGGAATTTTTAA

Coding sequence (CDS)

ATGAACAATAATCCCGATCTCCTCCAAACTTCCGCCGCCGGCGACACCAAGTACAGAGGAGTACGGCGGCGCAAATGGGGGAAATGGGTTGCTGAAATCCGTCTCCCTAATAGCCGCGACCGTATCTGGCTTGGTTCTTACGACTCACCAGAGAAAGCTGCCCGAGCCTTTGACGCTGCCCTTTTCTGTCTCCGTGGACCTCATGCCAAGTTTAACTTCCCTGACCAACCACTGCCCGACATTCTCAACGCTCATTCTCTCACTGCCCATCAGATCCAAGAATTGGCTGCGAAGTTCGCTAATGAATATCATCAAAACGACGACGTTGTGGTTGAGGGTATTGTAGTACCTCCAAATCCACCCACTGAGGACAATTGTGGAAATTCACCTTCCTCCTCCTCCAACAATATTAATAATTATAACAATATGGATTGGTCTTTCCTTGATGCCATTCATGAAGACCTTCCTCATTCTTCTTCTTCTTCCTCCAATTTCTTTCCTCTTTACAATGACCATTTTGATAATATCTTAACCAATGATTTTTATCAATCCACCAACAACAACGACCTTTACGACGTCGTTTCGGGTGATGACATTGACGACACTTTCTCCAATCAACCCTTTCTATGGAATTTTTAA

Protein sequence

MNNNPDLLQTSAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAALFCLRGPHAKFNFPDQPLPDILNAHSLTAHQIQELAAKFANEYHQNDDVVVEGIVVPPNPPTEDNCGNSPSSSSNNINNYNNMDWSFLDAIHEDLPHSSSSSSNFFPLYNDHFDNILTNDFYQSTNNNDLYDVVSGDDIDDTFSNQPFLWNF
BLAST of Lsi08G008230 vs. Swiss-Prot
Match: ERF18_ARATH (Ethylene-responsive transcription factor ERF018 OS=Arabidopsis thaliana GN=ERF018 PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 8.4e-31
Identity = 82/172 (47.67%), Postives = 106/172 (61.63%), Query Frame = 1

Query: 11  SAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAALFCLRGPHAK 70
           + A  +KY+GVR+RKWGKWV+EIRLP+SR+RIWLGSYD+PEKAARAFDAA FCLRG  A 
Sbjct: 14  NTAMQSKYKGVRKRKWGKWVSEIRLPHSRERIWLGSYDTPEKAARAFDAAQFCLRGGDAN 73

Query: 71  FNFPDQPLPDILNAHSLTAHQIQELAAKFANEYHQNDDVVVEGIVVPPNPPTEDNCGNSP 130
           FNFP+ P P I    SLT  +IQE AA+FAN +      +V+G       P  +    SP
Sbjct: 74  FNFPNNP-PSISVEKSLTPPEIQEAAARFANTFQD----IVKGEEESGLVPGSEIRPESP 133

Query: 131 SSSSNNINNYNNMDWSFLDAIHEDLPHSSSSSSNFFPLYNDHFDNILTNDFY 183
           S+S++   +  + D+SFLD +  +    S S         D F  IL  + Y
Sbjct: 134 STSASVATSTVDYDFSFLDLLPMNFGFDSFSDDFSGFSGGDRFTEILPIEDY 180

BLAST of Lsi08G008230 vs. Swiss-Prot
Match: ERF17_ARATH (Ethylene-responsive transcription factor ERF017 OS=Arabidopsis thaliana GN=ERF017 PE=2 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 3.5e-29
Identity = 80/203 (39.41%), Postives = 113/203 (55.67%), Query Frame = 1

Query: 10  TSAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAALFCLRGPHA 69
           +S++  +KY+GVR+RKWGKWV+EIRLPNSR+RIWLGSYD+PEKAARAFDAAL+CLRG +A
Sbjct: 4   SSSSMQSKYKGVRKRKWGKWVSEIRLPNSRERIWLGSYDTPEKAARAFDAALYCLRGNNA 63

Query: 70  KFNFPDQPLPDILNAHSLTAHQIQELAAKFANEYHQNDDVVVEGIVVPPNPPTEDNCGNS 129
           KFNFPD P P I    +L+  +I+E AA+FAN                 +   + + G  
Sbjct: 64  KFNFPDNP-PVISGGRNLSRSEIREAAARFAN-----------------SAEDDSSGGAG 123

Query: 130 PSSSSNNINNYNNMDWSFLDAIHEDLPHSSSSSSNFFPLYNDHFDNILTNDFYQSTNNND 189
                 + +   ++D  FL  +      + +S    FP ++D  D    + F +  +   
Sbjct: 124 YEIRQESASTSMDVDSEFLSMLPTVGSGNFASEFGLFPGFDDFSDEYSGDRFREQLSPTQ 183

Query: 190 LYDVVSGDDIDDTFSNQPFLWNF 213
            Y  +  +   D      FLWNF
Sbjct: 184 DYYQLGEETYAD---GSMFLWNF 185

BLAST of Lsi08G008230 vs. Swiss-Prot
Match: ERF16_ARATH (Ethylene-responsive transcription factor ERF016 OS=Arabidopsis thaliana GN=ERF016 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.0e-28
Identity = 91/221 (41.18%), Postives = 119/221 (53.85%), Query Frame = 1

Query: 17  KYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAALFCLRGPHAKFNFPDQ 76
           KY GVR+RKWGKWVAEIRLPNSRDRIWLGS+DS EKAARAFDAAL+CLRGP A+FNFPD 
Sbjct: 6   KYTGVRKRKWGKWVAEIRLPNSRDRIWLGSFDSAEKAARAFDAALYCLRGPGARFNFPDN 65

Query: 77  PLPDILNAHSLTAHQIQELAAKFA-----------------NEYHQNDDVVVEG---IVV 136
           P P+I    SLT  QIQ +A++FA                  +++  ++V++     I  
Sbjct: 66  P-PEIPGGRSLTPQQIQVVASRFACEEELLPPEQHHPSPPRGDHNTEEEVIISARGEINS 125

Query: 137 PPNPPTEDNCG--NSPSSSSNNINNYNNMDWSFLDAIHEDLPHSSSSSSNFFPLYNDHFD 196
               PT    G  N+   +SN+ ++Y  + W       E+      +S + F  + D   
Sbjct: 126 GSGGPTLGQVGEDNNNEGNSNDTSSYWPLIW------EEENFVGPPNSDHEFGFFTDDST 185

Query: 197 NIL--TNDFYQSTNNNDL-YDVVSGDDIDDTFSNQPFLWNF 213
           N+   T    Q   ++D  YD    DD      N   LWNF
Sbjct: 186 NLYFPTQQQQQHQLSSDFYYDGACEDDFSHYNIN---LWNF 216

BLAST of Lsi08G008230 vs. Swiss-Prot
Match: ERF15_ARATH (Ethylene-responsive transcription factor ERF015 OS=Arabidopsis thaliana GN=ERF015 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 2.7e-21
Identity = 47/86 (54.65%), Postives = 61/86 (70.93%), Query Frame = 1

Query: 18  YRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAALFCLRGPHAKFNFPDQP 77
           YRGVR+R WGKWV+EIR+P +  RIWLGSYD+PEKAARA+DAALFC+RG    +NFP   
Sbjct: 27  YRGVRKRSWGKWVSEIRVPKTGRRIWLGSYDAPEKAARAYDAALFCIRGEKGVYNFPTDK 86

Query: 78  LPDIL--NAHSLTAHQIQELAAKFAN 102
            P +   +   L+   IQ +A  +A+
Sbjct: 87  KPQLPEGSVRPLSKLDIQTIATNYAS 112

BLAST of Lsi08G008230 vs. Swiss-Prot
Match: RA210_ARATH (Ethylene-responsive transcription factor RAP2-10 OS=Arabidopsis thaliana GN=RAP2-10 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 6.7e-20
Identity = 42/61 (68.85%), Postives = 49/61 (80.33%), Query Frame = 1

Query: 15 DTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAALFCLRGPHAKFNFP 74
          D  Y+G+R RKWGKWVAEIR PN R RIWLGSY +PE AARA+D A+F LRGP A+ NFP
Sbjct: 27 DKPYKGIRMRKWGKWVAEIREPNKRSRIWLGSYSTPEAAARAYDTAVFYLRGPSARLNFP 86

Query: 75 D 76
          +
Sbjct: 87 E 87

BLAST of Lsi08G008230 vs. TrEMBL
Match: A0A0A0KIJ3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G361330 PE=4 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 6.6e-91
Identity = 182/223 (81.61%), Postives = 188/223 (84.30%), Query Frame = 1

Query: 1   MNNNPDLLQTSAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAA 60
           MNNN  + QTSAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAA
Sbjct: 1   MNNN--ISQTSAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAA 60

Query: 61  LFCLRGPHAKFNFPDQPLPDILNAHSLTAHQIQELAAKFANEY--HQNDDVVVEGIVVPP 120
           LFCLRGPHAKFNFPDQPLPDILNAHSLTAHQIQE AAKFANEY  HQNDDVV   +  PP
Sbjct: 61  LFCLRGPHAKFNFPDQPLPDILNAHSLTAHQIQEFAAKFANEYECHQNDDVV--DVAPPP 120

Query: 121 NPPT-EDNCGNSPSSSSNNINNYNNMDWSFLDAIHE-----DLPHSSSSSSNFFPLYNDH 180
              T E+ CG SPSSS     NYNNMDWSFLD IH+       PHSSSSSSNFFPLYNDH
Sbjct: 121 TTTTAEEKCGISPSSS-----NYNNMDWSFLDEIHDHHYPNPNPHSSSSSSNFFPLYNDH 180

Query: 181 FDNILTNDFYQST---NNNDLYDVVSGDDIDDTFSNQPFLWNF 213
           FDNILTNDFYQS+   NN+DLYD VSGDD DDTFSNQPFLWNF
Sbjct: 181 FDNILTNDFYQSSTTNNNDDLYDAVSGDD-DDTFSNQPFLWNF 213

BLAST of Lsi08G008230 vs. TrEMBL
Match: B9IKF6_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0018s08320g PE=4 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.2e-36
Identity = 98/208 (47.12%), Postives = 129/208 (62.02%), Query Frame = 1

Query: 9   QTSAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAALFCLRGPH 68
           Q+    + KY+GVR+RKWGKWV+EIRLPNSR+RIWLGSYD+P KAARAFDAAL+CLRG  
Sbjct: 11  QSDRNHEPKYKGVRKRKWGKWVSEIRLPNSRERIWLGSYDTPLKAARAFDAALYCLRGSG 70

Query: 69  AKFNFPDQPLPDILNAHSLTAHQIQELAAKFANEYHQNDDVVVEGIVVPPNPPTEDNCGN 128
           AKFNFPD P PDI+   SL++ +IQE+AA+FANE   +      G        + ++  N
Sbjct: 71  AKFNFPDNP-PDIVGGQSLSSQEIQEVAARFANEEPTSSSTATRG-------ESSEHVEN 130

Query: 129 SPSSSSNNINNYNNMDWSFLDAIHEDLPHSSSSSSNFFPLYN--DHFDNILTNDFYQSTN 188
             SSSS+     + +DWSFL+ +       SS  ++ F LY+  DH    +  D+Y    
Sbjct: 131 DTSSSSDG-GAGDTIDWSFLNLL------DSSEGASDFGLYHGLDH----MGGDYYPPPP 190

Query: 189 NNDLYDVVSGDDI--DDTFSNQPFLWNF 213
               Y  + GDD   D+ +S Q FLWNF
Sbjct: 191 PPSEYS-IGGDDHNGDEAYSQQSFLWNF 198

BLAST of Lsi08G008230 vs. TrEMBL
Match: A9PLA3_POPTR (RAP2-like protein OS=Populus trichocarpa GN=RAP2L13 PE=4 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 6.1e-36
Identity = 98/208 (47.12%), Postives = 129/208 (62.02%), Query Frame = 1

Query: 9   QTSAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAALFCLRGPH 68
           Q+    + KY+GVR+RKWGKWV+EIRLPNSR+RIWLGSYD+PEKAARAFDAAL+CLRG  
Sbjct: 16  QSDRTHEPKYKGVRKRKWGKWVSEIRLPNSRERIWLGSYDTPEKAARAFDAALYCLRGSG 75

Query: 69  AKFNFPDQPLPDILNAHSLTAHQIQELAAKFANEYHQNDDVVVEGIVVPPNPPTEDNCGN 128
           AKFNFPD P PDI+   SLT  ++QE+AA+FANE        + G     +    +N  +
Sbjct: 76  AKFNFPDNP-PDIVGGRSLTPQEVQEVAARFANE-EPTSSTAMGG----ESSSHVENYTS 135

Query: 129 SPSSSSNNINNYNNMDWSFLDAIHEDLPHSSSSSSNFFPLYN--DHFDNILTNDFYQSTN 188
           S S       + + +DWSFL+ +       S+  ++ F LY+  DH    +  D+Y    
Sbjct: 136 SSSDCGAGQMDSDTIDWSFLNLL------DSNEGASDFGLYHGLDH----MGGDYYPPPT 195

Query: 189 NNDLYDVVSGDDI--DDTFSNQPFLWNF 213
                D  +GDD   D+TFS+Q FLWNF
Sbjct: 196 PPPPPD-YNGDDNNGDETFSHQSFLWNF 206

BLAST of Lsi08G008230 vs. TrEMBL
Match: A0A068VAW6_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00011870001 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 5.7e-34
Identity = 95/214 (44.39%), Postives = 127/214 (59.35%), Query Frame = 1

Query: 11  SAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAALFCLRGPHAK 70
           S     KY+GVR+RKWGKWV+EIRLPNSR+RIWLGSYDS EKAARAFDAALFCLRG +AK
Sbjct: 18  SQEAQVKYKGVRKRKWGKWVSEIRLPNSRERIWLGSYDSAEKAARAFDAALFCLRGKNAK 77

Query: 71  FNFPDQPLPDILNAHSLTAHQIQELAAKFANEYHQNDDVVVEG---IVVPPNPPTEDNCG 130
           FNF D P PD++   SLT  +IQ +A+++AN Y   +    +G   I    + P + +  
Sbjct: 78  FNFADIP-PDLVGGRSLTPAEIQAVASEYANRYGNKEKQQQQGEGLIGEKEDDPLQFDQD 137

Query: 131 NSPSSSSNNINNYNNMDWSFLDAIHED-LPHSSSSSSNFFPLYNDHFDNILTNDFYQSTN 190
            SP  SSN+     N+DWSFL  +  +     ++S++  + L     DN L  D Y + +
Sbjct: 138 TSP-ISSNSDGALQNVDWSFLGMLEPNGAATGATSNAPDYDLMLSSLDN-LHEDMYMTPH 197

Query: 191 NNDLYDVVSG--------DDIDDTFSNQPFLWNF 213
                DV             +D+ +S+Q FLWNF
Sbjct: 198 VGTKVDVDHNFNGQHEDYQHLDENYSHQSFLWNF 228

BLAST of Lsi08G008230 vs. TrEMBL
Match: A0A072VD25_MEDTR (Ethylene-responsive transcription factor ERF018 OS=Medicago truncatula GN=MTR_1g014780 PE=4 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.6e-33
Identity = 99/215 (46.05%), Postives = 131/215 (60.93%), Query Frame = 1

Query: 9   QTSAAGDTK-YRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAALFCLRGP 68
           Q   + D K YRGVR+R WGKWV+EIRLPNSR+RIWLGSYD+ EKAARAFDAAL+CLRGP
Sbjct: 28  QNPNSSDNKIYRGVRKRNWGKWVSEIRLPNSRERIWLGSYDTQEKAARAFDAALYCLRGP 87

Query: 69  HAKFNFPDQPLP-DILNAH-----SLTAHQIQELAAKFANEYHQNDDVV-VEGIVVPPNP 128
           HA FNFP+ PL  ++   H     SL+  +IQE+AAKFANE     ++   E   VP +P
Sbjct: 88  HASFNFPNTPLTINVAVNHGVGHQSLSPEEIQEIAAKFANESSPPIELTQEEDHQVPSDP 147

Query: 129 PTEDNCGNSPSSS---SNNINNYNNMDWSFLDAIHEDLPHSSSSSSNFFPLYNDHFDNIL 188
               N  NS + S    N+I +   MDW+F D + ED+ +  ++ S+F+ L N  +    
Sbjct: 148 LESQNDTNSNAYSYPLDNDIGDLRRMDWTF-DDMFEDM-NGVANGSDFYGLQNMQYS--- 207

Query: 189 TNDFYQSTNNNDLYDVVSGDDIDDTFSNQPFLWNF 213
           T  F +        D V   + +DTF+N   LWN+
Sbjct: 208 TPPFEE--------DNVDQIECEDTFTNHSTLWNW 229

BLAST of Lsi08G008230 vs. TAIR10
Match: AT1G74930.1 (AT1G74930.1 Integrase-type DNA-binding superfamily protein)

HSP 1 Score: 135.2 bits (339), Expect = 4.7e-32
Identity = 82/172 (47.67%), Postives = 106/172 (61.63%), Query Frame = 1

Query: 11  SAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAALFCLRGPHAK 70
           + A  +KY+GVR+RKWGKWV+EIRLP+SR+RIWLGSYD+PEKAARAFDAA FCLRG  A 
Sbjct: 14  NTAMQSKYKGVRKRKWGKWVSEIRLPHSRERIWLGSYDTPEKAARAFDAAQFCLRGGDAN 73

Query: 71  FNFPDQPLPDILNAHSLTAHQIQELAAKFANEYHQNDDVVVEGIVVPPNPPTEDNCGNSP 130
           FNFP+ P P I    SLT  +IQE AA+FAN +      +V+G       P  +    SP
Sbjct: 74  FNFPNNP-PSISVEKSLTPPEIQEAAARFANTFQD----IVKGEEESGLVPGSEIRPESP 133

Query: 131 SSSSNNINNYNNMDWSFLDAIHEDLPHSSSSSSNFFPLYNDHFDNILTNDFY 183
           S+S++   +  + D+SFLD +  +    S S         D F  IL  + Y
Sbjct: 134 STSASVATSTVDYDFSFLDLLPMNFGFDSFSDDFSGFSGGDRFTEILPIEDY 180

BLAST of Lsi08G008230 vs. TAIR10
Match: AT1G19210.1 (AT1G19210.1 Integrase-type DNA-binding superfamily protein)

HSP 1 Score: 129.8 bits (325), Expect = 2.0e-30
Identity = 80/203 (39.41%), Postives = 113/203 (55.67%), Query Frame = 1

Query: 10  TSAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAALFCLRGPHA 69
           +S++  +KY+GVR+RKWGKWV+EIRLPNSR+RIWLGSYD+PEKAARAFDAAL+CLRG +A
Sbjct: 4   SSSSMQSKYKGVRKRKWGKWVSEIRLPNSRERIWLGSYDTPEKAARAFDAALYCLRGNNA 63

Query: 70  KFNFPDQPLPDILNAHSLTAHQIQELAAKFANEYHQNDDVVVEGIVVPPNPPTEDNCGNS 129
           KFNFPD P P I    +L+  +I+E AA+FAN                 +   + + G  
Sbjct: 64  KFNFPDNP-PVISGGRNLSRSEIREAAARFAN-----------------SAEDDSSGGAG 123

Query: 130 PSSSSNNINNYNNMDWSFLDAIHEDLPHSSSSSSNFFPLYNDHFDNILTNDFYQSTNNND 189
                 + +   ++D  FL  +      + +S    FP ++D  D    + F +  +   
Sbjct: 124 YEIRQESASTSMDVDSEFLSMLPTVGSGNFASEFGLFPGFDDFSDEYSGDRFREQLSPTQ 183

Query: 190 LYDVVSGDDIDDTFSNQPFLWNF 213
            Y  +  +   D      FLWNF
Sbjct: 184 DYYQLGEETYAD---GSMFLWNF 185

BLAST of Lsi08G008230 vs. TAIR10
Match: AT5G21960.1 (AT5G21960.1 Integrase-type DNA-binding superfamily protein)

HSP 1 Score: 128.3 bits (321), Expect = 5.8e-30
Identity = 91/221 (41.18%), Postives = 119/221 (53.85%), Query Frame = 1

Query: 17  KYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAALFCLRGPHAKFNFPDQ 76
           KY GVR+RKWGKWVAEIRLPNSRDRIWLGS+DS EKAARAFDAAL+CLRGP A+FNFPD 
Sbjct: 6   KYTGVRKRKWGKWVAEIRLPNSRDRIWLGSFDSAEKAARAFDAALYCLRGPGARFNFPDN 65

Query: 77  PLPDILNAHSLTAHQIQELAAKFA-----------------NEYHQNDDVVVEG---IVV 136
           P P+I    SLT  QIQ +A++FA                  +++  ++V++     I  
Sbjct: 66  P-PEIPGGRSLTPQQIQVVASRFACEEELLPPEQHHPSPPRGDHNTEEEVIISARGEINS 125

Query: 137 PPNPPTEDNCG--NSPSSSSNNINNYNNMDWSFLDAIHEDLPHSSSSSSNFFPLYNDHFD 196
               PT    G  N+   +SN+ ++Y  + W       E+      +S + F  + D   
Sbjct: 126 GSGGPTLGQVGEDNNNEGNSNDTSSYWPLIW------EEENFVGPPNSDHEFGFFTDDST 185

Query: 197 NIL--TNDFYQSTNNNDL-YDVVSGDDIDDTFSNQPFLWNF 213
           N+   T    Q   ++D  YD    DD      N   LWNF
Sbjct: 186 NLYFPTQQQQQHQLSSDFYYDGACEDDFSHYNIN---LWNF 216

BLAST of Lsi08G008230 vs. TAIR10
Match: AT4G31060.1 (AT4G31060.1 Integrase-type DNA-binding superfamily protein)

HSP 1 Score: 103.6 bits (257), Expect = 1.5e-22
Identity = 47/86 (54.65%), Postives = 61/86 (70.93%), Query Frame = 1

Query: 18  YRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAALFCLRGPHAKFNFPDQP 77
           YRGVR+R WGKWV+EIR+P +  RIWLGSYD+PEKAARA+DAALFC+RG    +NFP   
Sbjct: 27  YRGVRKRSWGKWVSEIRVPKTGRRIWLGSYDAPEKAARAYDAALFCIRGEKGVYNFPTDK 86

Query: 78  LPDIL--NAHSLTAHQIQELAAKFAN 102
            P +   +   L+   IQ +A  +A+
Sbjct: 87  KPQLPEGSVRPLSKLDIQTIATNYAS 112

BLAST of Lsi08G008230 vs. TAIR10
Match: AT4G36900.1 (AT4G36900.1 related to AP2 10)

HSP 1 Score: 99.0 bits (245), Expect = 3.8e-21
Identity = 42/61 (68.85%), Postives = 49/61 (80.33%), Query Frame = 1

Query: 15 DTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAALFCLRGPHAKFNFP 74
          D  Y+G+R RKWGKWVAEIR PN R RIWLGSY +PE AARA+D A+F LRGP A+ NFP
Sbjct: 27 DKPYKGIRMRKWGKWVAEIREPNKRSRIWLGSYSTPEAAARAYDTAVFYLRGPSARLNFP 86

Query: 75 D 76
          +
Sbjct: 87 E 87

BLAST of Lsi08G008230 vs. NCBI nr
Match: gi|449453065|ref|XP_004144279.1| (PREDICTED: ethylene-responsive transcription factor ERF017 [Cucumis sativus])

HSP 1 Score: 341.7 bits (875), Expect = 9.4e-91
Identity = 182/223 (81.61%), Postives = 188/223 (84.30%), Query Frame = 1

Query: 1   MNNNPDLLQTSAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAA 60
           MNNN  + QTSAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAA
Sbjct: 1   MNNN--ISQTSAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAA 60

Query: 61  LFCLRGPHAKFNFPDQPLPDILNAHSLTAHQIQELAAKFANEY--HQNDDVVVEGIVVPP 120
           LFCLRGPHAKFNFPDQPLPDILNAHSLTAHQIQE AAKFANEY  HQNDDVV   +  PP
Sbjct: 61  LFCLRGPHAKFNFPDQPLPDILNAHSLTAHQIQEFAAKFANEYECHQNDDVV--DVAPPP 120

Query: 121 NPPT-EDNCGNSPSSSSNNINNYNNMDWSFLDAIHE-----DLPHSSSSSSNFFPLYNDH 180
              T E+ CG SPSSS     NYNNMDWSFLD IH+       PHSSSSSSNFFPLYNDH
Sbjct: 121 TTTTAEEKCGISPSSS-----NYNNMDWSFLDEIHDHHYPNPNPHSSSSSSNFFPLYNDH 180

Query: 181 FDNILTNDFYQST---NNNDLYDVVSGDDIDDTFSNQPFLWNF 213
           FDNILTNDFYQS+   NN+DLYD VSGDD DDTFSNQPFLWNF
Sbjct: 181 FDNILTNDFYQSSTTNNNDDLYDAVSGDD-DDTFSNQPFLWNF 213

BLAST of Lsi08G008230 vs. NCBI nr
Match: gi|659133674|ref|XP_008466852.1| (PREDICTED: ethylene-responsive transcription factor ERF017-like [Cucumis melo])

HSP 1 Score: 337.0 bits (863), Expect = 2.3e-89
Identity = 178/222 (80.18%), Postives = 185/222 (83.33%), Query Frame = 1

Query: 1   MNNNPDLLQTSAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAA 60
           MNNN  L QTSAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAA
Sbjct: 1   MNNN--LSQTSAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAA 60

Query: 61  LFCLRGPHAKFNFPDQPLPDILNAHSLTAHQIQELAAKFANEY--HQNDDVVVEGIVVPP 120
           LFCLRGPHAKFNFP+QPLPDILNAHSLTAHQIQE AAKFANEY  HQND  VV+ +  PP
Sbjct: 61  LFCLRGPHAKFNFPEQPLPDILNAHSLTAHQIQEFAAKFANEYDCHQND--VVDDVAPPP 120

Query: 121 NPPT-----EDNCGNSPSSSSNNINNYNNMDWSFLDAIHEDLPHSSSSSSNFFPLYNDHF 180
              T     EDNCGNSPSSS     NYNN+DWSFLD IH+    +  SSSNFFPLYNDHF
Sbjct: 121 TTTTTTTTAEDNCGNSPSSS-----NYNNVDWSFLDEIHDHHNPNPHSSSNFFPLYNDHF 180

Query: 181 DNILTNDFYQST---NNNDLYDVVSGDDIDDTFSNQPFLWNF 213
           DNILTNDFYQS+   N NDLYD VSGDD DDTFSNQPFLWNF
Sbjct: 181 DNILTNDFYQSSTTNNTNDLYDAVSGDD-DDTFSNQPFLWNF 212

BLAST of Lsi08G008230 vs. NCBI nr
Match: gi|1012197059|ref|XP_015972153.1| (PREDICTED: ethylene-responsive transcription factor ERF017-like [Arachis duranensis])

HSP 1 Score: 164.1 bits (414), Expect = 2.7e-37
Identity = 96/230 (41.74%), Postives = 137/230 (59.57%), Query Frame = 1

Query: 7   LLQTSAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAALFCLRG 66
           ++ +S++    Y+GVR+RKWGKWV+EIRLPNSR+RIWLGSYD+ +KAARAFDAAL+CLRG
Sbjct: 23  VVDSSSSSCKMYKGVRKRKWGKWVSEIRLPNSRERIWLGSYDTQQKAARAFDAALYCLRG 82

Query: 67  PHAKFNFPDQPL----PDILNAHSLTAHQIQELAAKFANEYHQNDDVVVEGIVVPPNPPT 126
            HA FNFPD PL     ++ + HSL+ H+IQ++A+KFAN +   ++            P+
Sbjct: 83  RHASFNFPDTPLHLEINNVSHYHSLSHHEIQQVASKFANNFDAEEE----------EEPS 142

Query: 127 EDNCGNSPS-------SSSNNINNYNN-----------MDWSFLDAIHEDLPHSSSSSSN 186
             N  NS S       S++NN NN NN           MDW+FL+ + +    +  ++  
Sbjct: 143 SQNGANSASSVCDYDGSNNNNDNNNNNDNNNVQVDHGDMDWTFLNVLDDQNDSNFDNTIG 202

Query: 187 F-FPLYNDHFDNILTNDF-YQSTNNNDLYDVVSGDDIDDTFSNQPFLWNF 213
           +   LY    D + + +F Y +T      D  +GD  DD FSN  FLW++
Sbjct: 203 YDVGLYYGGIDKVYSGEFLYTTTPPTTFEDNNNGDADDDAFSNHSFLWSW 242

BLAST of Lsi08G008230 vs. NCBI nr
Match: gi|224142933|ref|XP_002324785.1| (hypothetical protein POPTR_0018s08320g [Populus trichocarpa])

HSP 1 Score: 161.4 bits (407), Expect = 1.7e-36
Identity = 98/208 (47.12%), Postives = 129/208 (62.02%), Query Frame = 1

Query: 9   QTSAAGDTKYRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAALFCLRGPH 68
           Q+    + KY+GVR+RKWGKWV+EIRLPNSR+RIWLGSYD+P KAARAFDAAL+CLRG  
Sbjct: 11  QSDRNHEPKYKGVRKRKWGKWVSEIRLPNSRERIWLGSYDTPLKAARAFDAALYCLRGSG 70

Query: 69  AKFNFPDQPLPDILNAHSLTAHQIQELAAKFANEYHQNDDVVVEGIVVPPNPPTEDNCGN 128
           AKFNFPD P PDI+   SL++ +IQE+AA+FANE   +      G        + ++  N
Sbjct: 71  AKFNFPDNP-PDIVGGQSLSSQEIQEVAARFANEEPTSSSTATRG-------ESSEHVEN 130

Query: 129 SPSSSSNNINNYNNMDWSFLDAIHEDLPHSSSSSSNFFPLYN--DHFDNILTNDFYQSTN 188
             SSSS+     + +DWSFL+ +       SS  ++ F LY+  DH    +  D+Y    
Sbjct: 131 DTSSSSDG-GAGDTIDWSFLNLL------DSSEGASDFGLYHGLDH----MGGDYYPPPP 190

Query: 189 NNDLYDVVSGDDI--DDTFSNQPFLWNF 213
               Y  + GDD   D+ +S Q FLWNF
Sbjct: 191 PPSEYS-IGGDDHNGDEAYSQQSFLWNF 198

BLAST of Lsi08G008230 vs. NCBI nr
Match: gi|1021528855|ref|XP_016207850.1| (PREDICTED: ethylene-responsive transcription factor ERF016-like [Arachis ipaensis])

HSP 1 Score: 161.0 bits (406), Expect = 2.3e-36
Identity = 97/219 (44.29%), Postives = 133/219 (60.73%), Query Frame = 1

Query: 18  YRGVRRRKWGKWVAEIRLPNSRDRIWLGSYDSPEKAARAFDAALFCLRGPHAKFNFPDQP 77
           Y+GVR+RKWGKWV+EIRLPNSR+RIWLGSYD+ +KAARAFDAAL+CLRG HA FNFPD P
Sbjct: 31  YKGVRKRKWGKWVSEIRLPNSRERIWLGSYDTQQKAARAFDAALYCLRGRHASFNFPDTP 90

Query: 78  L----PDILNAHSLTAHQIQELAAKFANEYHQNDDVVVEGIVVPPNPPTEDNCGNSPSS- 137
           L     ++ + HSL+ H+IQ++A+KFAN +   ++            P+  N  NS SS 
Sbjct: 91  LHLEINNVSHYHSLSHHEIQQVASKFANNFDAEEE----------EEPSSQNGANSASSV 150

Query: 138 -----SSNNINNYNN-----------MDWSFLDAIHEDLPHSSSSSSNF-FPLYNDHFDN 197
                S+NN NN +N           MDW+FL+ + +    +  ++  + F LY    D 
Sbjct: 151 CDYDGSNNNNNNDDNTNNNVQVDHGDMDWTFLNVLDDQNDSNFDNTIGYDFGLYYGGIDK 210

Query: 198 ILTNDF-YQSTNNNDLYDVVSGD-DIDDTFSNQPFLWNF 213
           + + +F Y +T      D  +GD D DD FSN  FLW++
Sbjct: 211 VHSGEFLYTTTPPTTFEDNNNGDADDDDAFSNHSFLWSW 239

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ERF18_ARATH8.4e-3147.67Ethylene-responsive transcription factor ERF018 OS=Arabidopsis thaliana GN=ERF01... [more]
ERF17_ARATH3.5e-2939.41Ethylene-responsive transcription factor ERF017 OS=Arabidopsis thaliana GN=ERF01... [more]
ERF16_ARATH1.0e-2841.18Ethylene-responsive transcription factor ERF016 OS=Arabidopsis thaliana GN=ERF01... [more]
ERF15_ARATH2.7e-2154.65Ethylene-responsive transcription factor ERF015 OS=Arabidopsis thaliana GN=ERF01... [more]
RA210_ARATH6.7e-2068.85Ethylene-responsive transcription factor RAP2-10 OS=Arabidopsis thaliana GN=RAP2... [more]
Match NameE-valueIdentityDescription
A0A0A0KIJ3_CUCSA6.6e-9181.61Uncharacterized protein OS=Cucumis sativus GN=Csa_6G361330 PE=4 SV=1[more]
B9IKF6_POPTR1.2e-3647.12Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0018s08320g PE=4 SV=1[more]
A9PLA3_POPTR6.1e-3647.12RAP2-like protein OS=Populus trichocarpa GN=RAP2L13 PE=4 SV=1[more]
A0A068VAW6_COFCA5.7e-3444.39Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00011870001 PE=4 SV=1[more]
A0A072VD25_MEDTR1.6e-3346.05Ethylene-responsive transcription factor ERF018 OS=Medicago truncatula GN=MTR_1g... [more]
Match NameE-valueIdentityDescription
AT1G74930.14.7e-3247.67 Integrase-type DNA-binding superfamily protein[more]
AT1G19210.12.0e-3039.41 Integrase-type DNA-binding superfamily protein[more]
AT5G21960.15.8e-3041.18 Integrase-type DNA-binding superfamily protein[more]
AT4G31060.11.5e-2254.65 Integrase-type DNA-binding superfamily protein[more]
AT4G36900.13.8e-2168.85 related to AP2 10[more]
Match NameE-valueIdentityDescription
gi|449453065|ref|XP_004144279.1|9.4e-9181.61PREDICTED: ethylene-responsive transcription factor ERF017 [Cucumis sativus][more]
gi|659133674|ref|XP_008466852.1|2.3e-8980.18PREDICTED: ethylene-responsive transcription factor ERF017-like [Cucumis melo][more]
gi|1012197059|ref|XP_015972153.1|2.7e-3741.74PREDICTED: ethylene-responsive transcription factor ERF017-like [Arachis duranen... [more]
gi|224142933|ref|XP_002324785.1|1.7e-3647.12hypothetical protein POPTR_0018s08320g [Populus trichocarpa][more]
gi|1021528855|ref|XP_016207850.1|2.3e-3644.29PREDICTED: ethylene-responsive transcription factor ERF016-like [Arachis ipaensi... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003677DNA binding
GO:0003700transcription factor activity, sequence-specific DNA binding
Vocabulary: Biological Process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
Vocabulary: INTERPRO
TermDefinition
IPR016177DNA-bd_dom_sf
IPR001471AP2/ERF_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0051301 cell division
biological_process GO:0002213 defense response to insect
biological_process GO:0010087 phloem or xylem histogenesis
biological_process GO:0009611 response to wounding
cellular_component GO:0005634 nucleus
cellular_component GO:0005667 transcription factor complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 transcription factor activity, sequence-specific DNA binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G008230.1Lsi08G008230.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 18..29
score: 2.1E-9coord: 40..56
score: 2.
IPR001471AP2/ERF domainGENE3DG3DSA:3.30.730.10coord: 17..75
score: 1.1
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 16..66
score: 2.9
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 17..80
score: 3.7
IPR001471AP2/ERF domainPROFILEPS51032AP2_ERFcoord: 17..74
score: 22
IPR016177DNA-binding domainunknownSSF54171DNA-binding domaincoord: 17..75
score: 1.57
NoneNo IPR availablePANTHERPTHR31985FAMILY NOT NAMEDcoord: 15..148
score: 8.8E-68coord: 164..212
score: 8.8
NoneNo IPR availablePANTHERPTHR31985:SF34ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF018coord: 164..212
score: 8.8E-68coord: 15..148
score: 8.8