Lsi09G004170 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi09G004170
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	L-lactate dehydrogenase
Location	chr09 : 4441686 .. 4444057 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGAAGAGTTCATCAACATCTTCTTTAGGTCCAGGCGGCCTTGACCTTTCTCAAGCCTTCTTCAAACCAATCCGCGACGCCTCTCCCCCATCGACTTCAAAGCGCAACTCCAAAATCTCCGTCATCGGCGCCGGGAACGTCGGCATGGCCATCGCTCAGACCATCCTCACTCAAGATCTTGTCGAAGAACTCGTCCTCGTCGACGCCAAGCCCGACAAACTCCGCGGCGAAATGCTCGATCTCCAACACGCTGCCGCCTTCCTCCCCCGAACCAAAATCAGCGCCTCCACCGATTACTCCATTACCGCTGGATCCGATCTATGTATCGTCACCGCTGGAGCTCGCCAGATCGCTGGTGAATCTAGGTTGAATCTGCTGCAGAGGAACGTTGCTCTGTTTCAGAAGATCGTGCCGCCGCTGGTTCAGTTCTCTCCCGAGACGATTCTTTTGATTGTTTCGAATCCGGTGGATGTTTTGACTTATGTTGCTTGGAAGCTCTCTGGTTTTCCGTCTAATCGAGTGATTGGATCCGGAACGAATCTGGATTCGTCTAGGTTTCGGTTTCTCATCGCCGATCATCTGGACGTCAATGCTCAGGATGTTCAGGTCAGTTTTCTGATTGACATTTCTTCTACCTTCTTCAATCAATCAGTGTTTATTTGTTAATTTGGTGTTGCTTTTTGAAGAAATTTCAATTTAGGGTTTGAAACCTTTCGATTTTTTCGTTTTTGGTTGATTACTTGATTAGTATCAAATGTGGCTGATAATTTTAGGGAAAATTTCGAACATTTCCCTAGAATTTGGAAGTTGGCAACTCGCCAACAAATCACAATAACTATTTGAAATATCTATCCGGAATATATGTTATGTGGATTAAATCTTATTTTCAACTTTCATATTTATTTAATTTTGATTTCTTAACTTTTTTGATGTTTATTTTAATCATTGAATTTTTTAATAATAAAACCTAAATTTTTTTTGGTTAAATTATAAGTTTAGTACTTTCATTTTTATGTTTGTGATTAACACGTCTTTGAACATTCAATTATATTTCTAATAGGTTTTTAACCTTTAAAATCATCGAATAGATCATTGAACTTTCAGATTTATGTCTAATAGATTATAATATAAATATATAATTTTAAAATGAACAAATCAATTAGCCATAATATAAAATTGATTCAATTACAAATTTAGTTCATAAACTTTGTGACTTCTGTCTATTTGACCTTTGAACTTTGAAATTACAAACTCAGTTTGAATTTTTCTTATATATACTTTTAAATTTCACGAATCTGCTAGACATAAAACTGAAAATTTGTAGTTTAATTAGACATAAAATTCAAATTTATATCAAATAGATTGAAATTTTATGAGCCTATTAGACACAAAAGTAAAAGTTTAGAGATCTATTAGACAATTTCAAAAAATCTTGAATAGGTTTAAGAACTATTTTACTTAAAACTAAATATGTAAGTTTATTAAACACAAAATCTAAAGTTTAGAGAGTTATTAGACACTTTTTAAAGTTGATAAATCTATTAAAATTGAGAGACTTATTTACCACAAACCAAAAAGTACATAGTCTAAACTTAGTAATTTAACTAAAAATGTAAAAATAACCAATTTTCTACCTAATATTTCTTCCCCAATACTCACCTCTGTTAAACTTGTTAATAGCAAAACAAAGATAAAGGACAATTATAGTTATTACTTTTAATGTTGAAGGAGAAAAATTAGAAAAAGAAGAAACAATAAATTCAAAGAATGAAAAAATAAATTCAAAGTTGACCCAAGTAGAATTCAAACCATCATATAGGTGCCTAACCTATGCATCAAACTATAGATGAATTTCTATAAATCAAACTAGCATTGATATTTAAAAGATGAAGCTAAAAAAAAGAGGATGATATATATGTTTGAAGGCATACATAGTGGGAGAGCATGGCGACAGCTCGGTGGCGCTATGGTCAAGCATAAGTGTGGGCGGGGTGCCAATACTGAGCTTTCTAAAGAATCAACAAATTGCCTACGAAAAAGAAACATTAGAGAAAATTCACAAAGAGGTCGTAGGCGGGGCCTATGAAGTCATAAGCCTGAAGGGCTACACATCATGGGCGATTGGGTACTCCGTGGCGAGCTTGGCTCGGTCGCTCCTTCGTGACCAACGGAGGATCCACCCCGTCTCTGTCCTTGCAAAAGGGTTCTACGGCATTGATGGTGGCGAGTTGTTCCTCAGCCTGCCTGCACAGCTCGGGAGGGGCGGAGTATTGGGGATCACCAATGTGCATCTAACGGCGGAGGAGTCGAGACGTTTGAGGGACTCGGCGAACACCATCTTGCAGGTGCAGACTGAATTGGGGATCTGA

mRNA sequence

ATGCAGAAGAGTTCATCAACATCTTCTTTAGGTCCAGGCGGCCTTGACCTTTCTCAAGCCTTCTTCAAACCAATCCGCGACGCCTCTCCCCCATCGACTTCAAAGCGCAACTCCAAAATCTCCGTCATCGGCGCCGGGAACGTCGGCATGGCCATCGCTCAGACCATCCTCACTCAAGATCTTGTCGAAGAACTCGTCCTCGTCGACGCCAAGCCCGACAAACTCCGCGGCGAAATGCTCGATCTCCAACACGCTGCCGCCTTCCTCCCCCGAACCAAAATCAGCGCCTCCACCGATTACTCCATTACCGCTGGATCCGATCTATGTATCGTCACCGCTGGAGCTCGCCAGATCGCTGGTGAATCTAGGTTGAATCTGCTGCAGAGGAACGTTGCTCTGTTTCAGAAGATCGTGCCGCCGCTGGTTCAGTTCTCTCCCGAGACGATTCTTTTGATTGTTTCGAATCCGGTGGATGTTTTGACTTATGTTGCTTGGAAGCTCTCTGGTTTTCCGTCTAATCGAGTGATTGGATCCGGAACGAATCTGGATTCGTCTAGGTTTCGGTTTCTCATCGCCGATCATCTGGACGTCAATGCTCAGGATGTTCAGGCATACATAGTGGGAGAGCATGGCGACAGCTCGGTGGCGCTATGGTCAAGCATAAGTGTGGGCGGGGTGCCAATACTGAGCTTTCTAAAGAATCAACAAATTGCCTACGAAAAAGAAACATTAGAGAAAATTCACAAAGAGGTCGTAGGCGGGGCCTATGAAGTCATAAGCCTGAAGGGCTACACATCATGGGCGATTGGGTACTCCGTGGCGAGCTTGGCTCGGTCGCTCCTTCGTGACCAACGGAGGATCCACCCCGTCTCTGTCCTTGCAAAAGGGTTCTACGGCATTGATGGTGGCGAGTTGTTCCTCAGCCTGCCTGCACAGCTCGGGAGGGGCGGAGTATTGGGGATCACCAATGTGCATCTAACGGCGGAGGAGTCGAGACGTTTGAGGGACTCGGCGAACACCATCTTGCAGGTGCAGACTGAATTGGGGATCTGA

Coding sequence (CDS)

ATGCAGAAGAGTTCATCAACATCTTCTTTAGGTCCAGGCGGCCTTGACCTTTCTCAAGCCTTCTTCAAACCAATCCGCGACGCCTCTCCCCCATCGACTTCAAAGCGCAACTCCAAAATCTCCGTCATCGGCGCCGGGAACGTCGGCATGGCCATCGCTCAGACCATCCTCACTCAAGATCTTGTCGAAGAACTCGTCCTCGTCGACGCCAAGCCCGACAAACTCCGCGGCGAAATGCTCGATCTCCAACACGCTGCCGCCTTCCTCCCCCGAACCAAAATCAGCGCCTCCACCGATTACTCCATTACCGCTGGATCCGATCTATGTATCGTCACCGCTGGAGCTCGCCAGATCGCTGGTGAATCTAGGTTGAATCTGCTGCAGAGGAACGTTGCTCTGTTTCAGAAGATCGTGCCGCCGCTGGTTCAGTTCTCTCCCGAGACGATTCTTTTGATTGTTTCGAATCCGGTGGATGTTTTGACTTATGTTGCTTGGAAGCTCTCTGGTTTTCCGTCTAATCGAGTGATTGGATCCGGAACGAATCTGGATTCGTCTAGGTTTCGGTTTCTCATCGCCGATCATCTGGACGTCAATGCTCAGGATGTTCAGGCATACATAGTGGGAGAGCATGGCGACAGCTCGGTGGCGCTATGGTCAAGCATAAGTGTGGGCGGGGTGCCAATACTGAGCTTTCTAAAGAATCAACAAATTGCCTACGAAAAAGAAACATTAGAGAAAATTCACAAAGAGGTCGTAGGCGGGGCCTATGAAGTCATAAGCCTGAAGGGCTACACATCATGGGCGATTGGGTACTCCGTGGCGAGCTTGGCTCGGTCGCTCCTTCGTGACCAACGGAGGATCCACCCCGTCTCTGTCCTTGCAAAAGGGTTCTACGGCATTGATGGTGGCGAGTTGTTCCTCAGCCTGCCTGCACAGCTCGGGAGGGGCGGAGTATTGGGGATCACCAATGTGCATCTAACGGCGGAGGAGTCGAGACGTTTGAGGGACTCGGCGAACACCATCTTGCAGGTGCAGACTGAATTGGGGATCTGA

Protein sequence

MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQDLVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYEKETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGIDGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI

Homology

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BLAST of Lsi09G004170 vs. Swiss-Prot
Match: LDHA_HORVU (L-lactate dehydrogenase A OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 8.5e-129
Identity = 243/348 (69.83%), Postives = 287/348 (82.47%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASP--PSTS----KRNSKISVIGAGNVGMAIAQ 60
           M K+SS S LG      S  FF+P+ D  P  P++S    +R +KISVIGAGNVGMAIAQ
Sbjct: 1   MHKASSLSELGFDAGGASSGFFRPVADGCPATPTSSAVPHRRLTKISVIGAGNVGMAIAQ 60

Query: 61  TILTQDLVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAG 120
           TILTQ+L +E+ LVDA PDKLRGE LDLQHAAAFLPR +IS  TD ++T  SDL IVTAG
Sbjct: 61  TILTQNLADEIALVDALPDKLRGEALDLQHAAAFLPRVRIS-GTDAAVTKNSDLVIVTAG 120

Query: 121 ARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNR 180
           ARQI GE+RLNLLQRNVAL++KIVPP+ + SP+ +LL+VSNPVDVLTYVAWKLSGFP++R
Sbjct: 121 ARQIPGETRLNLLQRNVALYRKIVPPVAEHSPDALLLVVSNPVDVLTYVAWKLSGFPASR 180

Query: 181 VIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKN 240
           VIGSGTNLDSSRFRFLIA+HLDVNAQDVQAY+VGEHGDSSVA+WSSISVGG+P    L++
Sbjct: 181 VIGSGTNLDSSRFRFLIAEHLDVNAQDVQAYMVGEHGDSSVAIWSSISVGGMPAFKSLRD 240

Query: 241 QQIAYEKETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLA 300
              ++++  LE I + VVGGAYEVI LKGYTSWAIGYSVASLA SLLRDQRR+HPVSVLA
Sbjct: 241 SHRSFDEAALEGIRRAVVGGAYEVIGLKGYTSWAIGYSVASLAASLLRDQRRVHPVSVLA 300

Query: 301 KGFYGI-DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTI 342
            GF+GI DG E+FLSLPA+LGRGG+LG+  + LT  E+ +LR SA T+
Sbjct: 301 SGFHGISDGHEVFLSLPARLGRGGILGVAEMDLTEAEAAQLRRSAKTL 347

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BLAST of Lsi09G004170 vs. Swiss-Prot
Match: LDHB_HORVU (L-lactate dehydrogenase B (Fragment) OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.1e-125
Identity = 232/342 (67.84%), Postives = 280/342 (81.87%), Query Frame = 1


Query: 16  DLSQAFFKPIRDASP------PSTSKRNSKISVIGAGNVGMAIAQTILTQDLVEELVLVD 75
           D S  FF+ + D  P       +  +R +K+SVIGAGNVGMAIAQTILTQ+L +E+ LVD
Sbjct: 3   DASSGFFRAVADGCPITHTSCSAPHRRLTKVSVIGAGNVGMAIAQTILTQNLADEIALVD 62

Query: 76  AKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAGESRLNLLQR 135
           A PDKLRGE LDLQHAAAFLPR +I + TD ++T  SDL +VTAGARQI GE+RLNLLQR
Sbjct: 63  ALPDKLRGEALDLQHAAAFLPRVRIVSGTDAAVTKNSDLIVVTAGARQIPGETRLNLLQR 122

Query: 136 NVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGTNLDSSRFRF 195
           NVAL++KIVPP+ + SP+ +LL+VSNPVDVLTYVAWKLSGFP++RVIGSGTNLDSSRFRF
Sbjct: 123 NVALYRKIVPPVAEHSPDALLLVVSNPVDVLTYVAWKLSGFPASRVIGSGTNLDSSRFRF 182

Query: 196 LIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYEKETLEKIHK 255
           L+A+HLDV+AQDVQAY+VGEHGDSSVA+WSSISVGG+P L  L++   ++++  LE I +
Sbjct: 183 LVAEHLDVSAQDVQAYMVGEHGDSSVAIWSSISVGGMPALKSLRDSHRSFDEAALEGIRR 242

Query: 256 EVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI-DGGELFLS 315
            VVGGAYEVI LKGYTSWAIGYSVASLA SLLRDQRR+HPVSVLA GF+GI DG E+FLS
Sbjct: 243 AVVGGAYEVIGLKGYTSWAIGYSVASLATSLLRDQRRVHPVSVLAAGFHGISDGHEVFLS 302

Query: 316 LPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           LPA+LGR GVLG+  + LT  E+ +LR SA T+ +    LG+
Sbjct: 303 LPARLGRAGVLGVAEMDLTEAEAAQLRRSAKTLWENCQLLGL 344

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BLAST of Lsi09G004170 vs. Swiss-Prot
Match: LDH_MAIZE (L-lactate dehydrogenase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 2.9e-121
Identity = 236/355 (66.48%), Postives = 284/355 (80.00%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIR-DASPPSTS---KRNSKISVIGAGNVGMAIAQTI 60
           M+K++S S LG    D S  FF+P+  D+S P++    +R +K+SVIGAGNVGMAIAQTI
Sbjct: 1   MKKATSLSELGFDAGDASSGFFRPVSGDSSTPTSQHHRRRLTKVSVIGAGNVGMAIAQTI 60

Query: 61  LTQDLVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGAR 120
           LT+DL +E+ LVDA PDKLRGEMLDLQHAAAFLPRT++ + TD S+T GSDL IVTAGAR
Sbjct: 61  LTRDLADEIALVDAVPDKLRGEMLDLQHAAAFLPRTRLVSGTDMSVTRGSDLVIVTAGAR 120

Query: 121 QIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVI 180
           QI GE+RL+LLQRNVALF+KIVPPL + S + +LL+VSNPVDVLTYVAWKLSGFP++RVI
Sbjct: 121 QIQGETRLDLLQRNVALFRKIVPPLAEQSHDALLLVVSNPVDVLTYVAWKLSGFPASRVI 180

Query: 181 GSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQ 240
           GSGTNLDSSRFRFL+A+HLDVNAQDVQAY+VGEHGDSSVA+WSS+SV G+P+L  L+   
Sbjct: 181 GSGTNLDSSRFRFLLAEHLDVNAQDVQAYMVGEHGDSSVAVWSSVSVAGMPVLKSLQESH 240

Query: 241 IAYEKETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKG 300
             +++E LE I + VV  AYEVISLKGYTSWAIGYSVASLA SLLRDQRRIHPVSVLA+G
Sbjct: 241 RCFDEEALEGIRRAVVDSAYEVISLKGYTSWAIGYSVASLAASLLRDQRRIHPVSVLARG 300

Query: 301 FYGI-DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           F+GI DG     + P +  R    G   + LT EE++RLR SA TI +    LG+
Sbjct: 301 FHGIPDGTTSSSACPPRRPRRRP-GRREMELTEEEAKRLRRSAKTIWENCQLLGL 354

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BLAST of Lsi09G004170 vs. Swiss-Prot
Match: LDHA_MOUSE (L-lactate dehydrogenase A chain OS=Mus musculus GN=Ldha PE=1 SV=3)

HSP 1 Score: 342.4 bits (877), Expect = 5.7e-93
Identity = 173/311 (55.63%), Postives = 229/311 (73.63%), Query Frame = 1


Query: 38  SKISVIGAGNVGMAIAQTILTQDLVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISAS 97
           +KI+V+G G VGMA A +IL +DL +EL LVD   DKL+GEM+DLQH + FL   KI +S
Sbjct: 21  NKITVVGVGAVGMACAISILMKDLADELALVDVMEDKLKGEMMDLQHGSLFLKTPKIVSS 80

Query: 98  TDYSITAGSDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPV 157
            DY +TA S L I+TAGARQ  GESRLNL+QRNV +F+ I+P +V++SP   LLIVSNPV
Sbjct: 81  KDYCVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNIVKYSPHCKLLIVSNPV 140

Query: 158 DVLTYVAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVAL 217
           D+LTYVAWK+SGFP NRVIGSG NLDS+RFR+L+ + L V+A     +++GEHGDSSV +
Sbjct: 141 DILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHALSCHGWVLGEHGDSSVPV 200

Query: 218 WSSISVGGVPILSFLKNQQIAYEKETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLA 277
           WS ++V GV + S         +KE  +++HK+VV  AYEVI LKGYTSWAIG SVA LA
Sbjct: 201 WSGVNVAGVSLKSLNPELGTDADKEQWKEVHKQVVDSAYEVIKLKGYTSWAIGLSVADLA 260

Query: 278 RSLLRDQRRIHPVSVLAKGFYGIDGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDS 337
            S++++ RR+HP+S + KG YGI+  ++FLS+P  LG+ G+  +  V LT EE  RL+ S
Sbjct: 261 ESIMKNLRRVHPISTMIKGLYGIN-EDVFLSVPCILGQNGISDVVKVTLTPEEEARLKKS 320

Query: 338 ANTILQVQTEL 349
           A+T+  +Q EL
Sbjct: 321 ADTLWGIQKEL 330

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BLAST of Lsi09G004170 vs. Swiss-Prot
Match: LDHA_RAT (L-lactate dehydrogenase A chain OS=Rattus norvegicus GN=Ldha PE=1 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 4.8e-92
Identity = 172/311 (55.31%), Postives = 228/311 (73.31%), Query Frame = 1


Query: 38  SKISVIGAGNVGMAIAQTILTQDLVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISAS 97
           +KI+V+G G VGMA A +IL +DL +EL LVD   DKL+GEM+DLQH + FL   KI +S
Sbjct: 21  NKITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGSLFLKTPKIVSS 80

Query: 98  TDYSITAGSDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPV 157
            DYS+TA S L I+TAGARQ  GESRLNL+QRNV +F+ I+P +V++SP+  LLIVSNPV
Sbjct: 81  KDYSVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNVVKYSPQCKLLIVSNPV 140

Query: 158 DVLTYVAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVAL 217
           D+LTYVAWK+SGFP NRVIGSG NLDS+RFR+L+ + L V+      +++GEHGDSSV +
Sbjct: 141 DILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHPLSCHGWVLGEHGDSSVPV 200

Query: 218 WSSISVGGVPILSFLKNQQIAYEKETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLA 277
           WS ++V GV + S         +KE  + +HK+VV  AYEVI LKGYTSWAIG SVA LA
Sbjct: 201 WSGVNVAGVSLKSLNPQLGTDADKEQWKDVHKQVVDSAYEVIKLKGYTSWAIGLSVADLA 260

Query: 278 RSLLRDQRRIHPVSVLAKGFYGIDGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDS 337
            S++++ RR+HP+S + KG YGI   ++FLS+P  LG+ G+  +  V LT +E  RL+ S
Sbjct: 261 ESIMKNLRRVHPISTMIKGLYGIK-EDVFLSVPCILGQNGISDVVKVTLTPDEEARLKKS 320

Query: 338 ANTILQVQTEL 349
           A+T+  +Q EL
Sbjct: 321 ADTLWGIQKEL 330

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BLAST of Lsi09G004170 vs. TrEMBL
Match: A0A0A0KKU9_CUCSA (L-lactate dehydrogenase OS=Cucumis sativus GN=Csa_5G161920 PE=3 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 2.0e-177
Identity = 327/350 (93.43%), Postives = 337/350 (96.29%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS STSSLGPGGLDLSQAFFKPIRDASP STS  N+KISVIG GNVGMAIAQTILTQD
Sbjct: 63  MQKSPSTSSLGPGGLDLSQAFFKPIRDASPLSTSNCNTKISVIGTGNVGMAIAQTILTQD 122

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LV+ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITA SDLCIVTAGARQIAG
Sbjct: 123 LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITARSDLCIVTAGARQIAG 182

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNV LFQKIVP LVQFSPETILLIVSNPVDVLTYVAWKLSGF SNRVIGSGT
Sbjct: 183 ESRLNLLQRNVTLFQKIVPLLVQFSPETILLIVSNPVDVLTYVAWKLSGFSSNRVIGSGT 242

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFLKNQQI YE
Sbjct: 243 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 302

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEV+GGAYEVI+LKGYTSWAIGYSVASLARS+LRDQRRIHPVSV+AKGFYGI
Sbjct: 303 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVIAKGFYGI 362

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           +GG+LFLSLPAQLGRGGVLGITNVHLT EES+RLRDSANTIL VQ ELGI
Sbjct: 363 EGGDLFLSLPAQLGRGGVLGITNVHLTTEESKRLRDSANTILHVQAELGI 412

[Close]

BLAST of Lsi09G004170 vs. TrEMBL
Match: A0A067KUG8_JATCU (L-lactate dehydrogenase OS=Jatropha curcas GN=JCGZ_03382 PE=3 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 1.7e-168
Identity = 301/350 (86.00%), Postives = 331/350 (94.57%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS S S+LGPGGLDL+QAFFKPI++ +PPS +KR++KISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASTLGPGGLDLTQAFFKPIQNTAPPSPTKRHTKISVIGTGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L +EL LVDAKPDKLRGEMLDLQHAAAFLPRTKI ASTDYS+T GSDLCIVTAGARQI G
Sbjct: 61  LADELALVDAKPDKLRGEMLDLQHAAAFLPRTKIIASTDYSVTVGSDLCIVTAGARQIPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF+KI+PPLV +SPE+IL+IVSNPVDVLTY+AWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFEKIIPPLVNYSPESILMIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFL+ QQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLEKQQIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLE IHK V+  AYEVI+LKGYTSWAIGYS A+LARSLLRDQR+IHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKAVIDSAYEVINLKGYTSWAIGYSAANLARSLLRDQRKIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGGE+FLSLPAQLGRGGVLG+TNVHLT EE+RRLR+SA T+L+VQ++LGI
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNVHLTEEEARRLRESAKTLLEVQSQLGI 350

[Close]

BLAST of Lsi09G004170 vs. TrEMBL
Match: A0A0D2UW00_GOSRA (L-lactate dehydrogenase OS=Gossypium raimondii GN=B456_009G297700 PE=3 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 6.0e-166
Identity = 298/350 (85.14%), Postives = 328/350 (93.71%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDL+QAFFKPI++  PPS++KR++KISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASSLGPGGLDLAQAFFKPIQNCDPPSSTKRHTKISVIGVGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L +EL LVDAK DKLRGEMLDLQHAAAFLPRTKI+AS DYS+TAGSDLCIVTAGARQ  G
Sbjct: 61  LADELALVDAKSDKLRGEMLDLQHAAAFLPRTKINASVDYSVTAGSDLCIVTAGARQNPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF  I+PPL ++SP++ILLIVSNPVDVLTYV+WKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFSNIIPPLAKYSPDSILLIVSNPVDVLTYVSWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSIS+GGVP+LSFLKNQQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISIGGVPVLSFLKNQQIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           K+TLE IHK VV  AYEVISLKGYTSWAIGYSVA+LARSLLRDQR+IHPVSVLAKGFYGI
Sbjct: 241 KQTLENIHKAVVESAYEVISLKGYTSWAIGYSVANLARSLLRDQRKIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGGE+FLSLPAQLGRGGVLG+TN+HLT EE +RLR SA TIL+VQ++LG+
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNIHLTDEEVQRLRKSAETILEVQSQLGL 350

[Close]

BLAST of Lsi09G004170 vs. TrEMBL
Match: A0A151TJG6_CAJCA (L-lactate dehydrogenase OS=Cajanus cajan GN=KK1_013505 PE=3 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 7.9e-166
Identity = 299/350 (85.43%), Postives = 330/350 (94.29%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           M KS S+S+LGPGGLDL+QAFFK I +A+P S +KR++KISV+GAGNVGMAIAQTILTQD
Sbjct: 1   MHKSGSSSALGPGGLDLTQAFFKSIMNAAPSSPTKRHNKISVVGAGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L +EL LVDA P+KLRGEMLDLQHAAAFLPRTKI ASTDY++TAGSDLCIVTAGARQI G
Sbjct: 61  LTDELALVDAIPEKLRGEMLDLQHAAAFLPRTKIHASTDYAVTAGSDLCIVTAGARQING 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNV LFQKI+PPLV  SP+TILLIVSNPVDVL+YVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVTLFQKIIPPLVGHSPDTILLIVSNPVDVLSYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVP+LSFL+ Q+IAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPVLSFLEKQEIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           +E LEKIHKEV+ GAYEVISLKGYTSWAIGYSVA+LARS+LR+QRR+HPVSVLAKGFYGI
Sbjct: 241 REMLEKIHKEVIQGAYEVISLKGYTSWAIGYSVANLARSILRNQRRVHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGGE+FLSLPAQLGRGGVLG+TNVHLT EES+RLRDSANTIL+VQ +L I
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNVHLTEEESQRLRDSANTILEVQNQLAI 350

[Close]

BLAST of Lsi09G004170 vs. TrEMBL
Match: A0A0B0P6A6_GOSAR (L-lactate dehydrogenase OS=Gossypium arboreum GN=F383_25947 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 1.3e-165
Identity = 298/350 (85.14%), Postives = 327/350 (93.43%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDL+QAFFKPI +  PPS++KR++KISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASSLGPGGLDLAQAFFKPILNTDPPSSTKRHTKISVIGVGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L +EL LVDAK DKLRGEMLDLQHAAAFLPRTKI+AS DYS+TAGSDLCIVTAGARQ  G
Sbjct: 61  LADELALVDAKSDKLRGEMLDLQHAAAFLPRTKINASVDYSVTAGSDLCIVTAGARQNPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF  I+PPL ++SP++ILLIVSNPVDVLTYV+WKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFSNIIPPLAKYSPDSILLIVSNPVDVLTYVSWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSIS+GGVP+LSFLKNQQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISIGGVPVLSFLKNQQIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           K+TLE IHK VV  AYEVISLKGYTSWAIGYSVA+LARSLLRDQR+IHPVSVLAKGFYGI
Sbjct: 241 KQTLENIHKAVVESAYEVISLKGYTSWAIGYSVANLARSLLRDQRKIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGGE+FLSLPAQLGRGGVLG+TN+HLT EE +RLR SA TIL+VQ++LG+
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNIHLTDEEVQRLRKSAETILEVQSQLGL 350

[Close]

BLAST of Lsi09G004170 vs. TAIR10
Match: AT4G17260.1 (AT4G17260.1 Lactate/malate dehydrogenase family protein)

HSP 1 Score: 546.6 bits (1407), Expect = 1.1e-155
Identity = 271/353 (76.77%), Postives = 317/353 (89.80%), Query Frame = 1


Query: 1   MQKSSSTSSL---GPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTIL 60
           M+K++STSSL   GP GLDL+ AFFKPI ++ P   S R +K+SV+G GNVGMAIAQTIL
Sbjct: 1   MEKNASTSSLKDLGPSGLDLTSAFFKPIHNSDPSLPSNRRTKVSVVGVGNVGMAIAQTIL 60

Query: 61  TQDLVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQ 120
           TQDL +E+ LVDAKPDKLRGEMLDLQHAAAFLPRTKI+AS DY +TAGSDLCIVTAGARQ
Sbjct: 61  TQDLADEIALVDAKPDKLRGEMLDLQHAAAFLPRTKITASVDYEVTAGSDLCIVTAGARQ 120

Query: 121 IAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIG 180
             GESRLNLLQRNVALF+ I+PPL + SP++IL+IVSNPVDVLTYVAWKLSGFP NRV+G
Sbjct: 121 NPGESRLNLLQRNVALFRHIIPPLAKASPDSILIIVSNPVDVLTYVAWKLSGFPVNRVLG 180

Query: 181 SGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQI 240
           SGTNLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGG+P+LSFL+  QI
Sbjct: 181 SGTNLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGIPVLSFLEKNQI 240

Query: 241 AYEKETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGF 300
           AYEK+TLE IH+ VVG AYEVI LKGYTSWAIGYSVA+LAR++LRDQR+IHPV+VLA+GF
Sbjct: 241 AYEKQTLEDIHQAVVGSAYEVIGLKGYTSWAIGYSVANLARTILRDQRKIHPVTVLARGF 300

Query: 301 YGIDGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           YG+DGG++FLSLPA LGR GV+ +TNVH+T EE+ +L+ SA TIL++Q++LG+
Sbjct: 301 YGVDGGDVFLSLPALLGRNGVVAVTNVHMTDEEAEKLQKSAKTILEMQSQLGL 353

[Close]

BLAST of Lsi09G004170 vs. NCBI nr
Match: gi|659073513|ref|XP_008437100.1| (PREDICTED: L-lactate dehydrogenase B [Cucumis melo])

HSP 1 Score: 656.8 bits (1693), Expect = 2.2e-185
Identity = 339/350 (96.86%), Postives = 347/350 (99.14%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS STSSLGPGGLDLSQAFFKPIRDASPPSTS RN+KISVIGAGNVGMAIAQTILTQD
Sbjct: 83  MQKSPSTSSLGPGGLDLSQAFFKPIRDASPPSTSNRNTKISVIGAGNVGMAIAQTILTQD 142

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LV+ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYS TAGS+LCIVTAGARQIAG
Sbjct: 143 LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSTTAGSNLCIVTAGARQIAG 202

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 203 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 262

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE
Sbjct: 263 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 322

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEV+GGAYEVI+LKGYTSWAIGYSVASLARS+LRDQRRIHPVSVLAKGFYGI
Sbjct: 323 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 382

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGG+LFLSLPAQLGRGGVLGITNVHLTAEES+RLRDSANTILQVQTELGI
Sbjct: 383 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 432

[Close]

BLAST of Lsi09G004170 vs. NCBI nr
Match: gi|778700018|ref|XP_004151663.2| (PREDICTED: LOW QUALITY PROTEIN: L-lactate dehydrogenase A-like [Cucumis sativus])

HSP 1 Score: 651.0 bits (1678), Expect = 1.2e-183
Identity = 335/350 (95.71%), Postives = 345/350 (98.57%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKSSS SSLGPGGLDLSQAFFKPIRDASPPST+ RN+KISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LV+ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVT GARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTY+AWKLSGFPSNRVI SGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIXSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NL+SSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQI YE
Sbjct: 181 NLNSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEV+GGAYEVI+LKGYTSWAIGYSVASLARS+LRDQRRIHPVSVLAKGFYGI
Sbjct: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGG+LFLSLPAQLGRGGVLGITNVHLTAEES+RLRDSANTILQVQTELGI
Sbjct: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 350

[Close]

BLAST of Lsi09G004170 vs. NCBI nr
Match: gi|778700014|ref|XP_011654796.1| (PREDICTED: L-lactate dehydrogenase B-like [Cucumis sativus])

HSP 1 Score: 629.8 bits (1623), Expect = 2.9e-177
Identity = 327/350 (93.43%), Postives = 337/350 (96.29%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS STSSLGPGGLDLSQAFFKPIRDASP STS  N+KISVIG GNVGMAIAQTILTQD
Sbjct: 63  MQKSPSTSSLGPGGLDLSQAFFKPIRDASPLSTSNCNTKISVIGTGNVGMAIAQTILTQD 122

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LV+ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITA SDLCIVTAGARQIAG
Sbjct: 123 LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITARSDLCIVTAGARQIAG 182

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNV LFQKIVP LVQFSPETILLIVSNPVDVLTYVAWKLSGF SNRVIGSGT
Sbjct: 183 ESRLNLLQRNVTLFQKIVPLLVQFSPETILLIVSNPVDVLTYVAWKLSGFSSNRVIGSGT 242

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFLKNQQI YE
Sbjct: 243 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 302

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEV+GGAYEVI+LKGYTSWAIGYSVASLARS+LRDQRRIHPVSV+AKGFYGI
Sbjct: 303 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVIAKGFYGI 362

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           +GG+LFLSLPAQLGRGGVLGITNVHLT EES+RLRDSANTIL VQ ELGI
Sbjct: 363 EGGDLFLSLPAQLGRGGVLGITNVHLTTEESKRLRDSANTILHVQAELGI 412

[Close]

BLAST of Lsi09G004170 vs. NCBI nr
Match: gi|802581859|ref|XP_012069951.1| (PREDICTED: L-lactate dehydrogenase B [Jatropha curcas])

HSP 1 Score: 600.1 bits (1546), Expect = 2.4e-168
Identity = 301/350 (86.00%), Postives = 331/350 (94.57%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS S S+LGPGGLDL+QAFFKPI++ +PPS +KR++KISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASTLGPGGLDLTQAFFKPIQNTAPPSPTKRHTKISVIGTGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L +EL LVDAKPDKLRGEMLDLQHAAAFLPRTKI ASTDYS+T GSDLCIVTAGARQI G
Sbjct: 61  LADELALVDAKPDKLRGEMLDLQHAAAFLPRTKIIASTDYSVTVGSDLCIVTAGARQIPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF+KI+PPLV +SPE+IL+IVSNPVDVLTY+AWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFEKIIPPLVNYSPESILMIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFL+ QQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLEKQQIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLE IHK V+  AYEVI+LKGYTSWAIGYS A+LARSLLRDQR+IHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKAVIDSAYEVINLKGYTSWAIGYSAANLARSLLRDQRKIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGGE+FLSLPAQLGRGGVLG+TNVHLT EE+RRLR+SA T+L+VQ++LGI
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNVHLTEEEARRLRESAKTLLEVQSQLGI 350

[Close]

BLAST of Lsi09G004170 vs. NCBI nr
Match: gi|823228917|ref|XP_012447203.1| (PREDICTED: L-lactate dehydrogenase B-like [Gossypium raimondii])

HSP 1 Score: 591.7 bits (1524), Expect = 8.7e-166
Identity = 298/350 (85.14%), Postives = 328/350 (93.71%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDL+QAFFKPI++  PPS++KR++KISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASSLGPGGLDLAQAFFKPIQNCDPPSSTKRHTKISVIGVGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L +EL LVDAK DKLRGEMLDLQHAAAFLPRTKI+AS DYS+TAGSDLCIVTAGARQ  G
Sbjct: 61  LADELALVDAKSDKLRGEMLDLQHAAAFLPRTKINASVDYSVTAGSDLCIVTAGARQNPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF  I+PPL ++SP++ILLIVSNPVDVLTYV+WKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFSNIIPPLAKYSPDSILLIVSNPVDVLTYVSWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSIS+GGVP+LSFLKNQQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISIGGVPVLSFLKNQQIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           K+TLE IHK VV  AYEVISLKGYTSWAIGYSVA+LARSLLRDQR+IHPVSVLAKGFYGI
Sbjct: 241 KQTLENIHKAVVESAYEVISLKGYTSWAIGYSVANLARSLLRDQRKIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGGE+FLSLPAQLGRGGVLG+TN+HLT EE +RLR SA TIL+VQ++LG+
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNIHLTDEEVQRLRKSAETILEVQSQLGL 350

The following BLAST results are available for this feature:

BLAST of Lsi09G004170 vs. Swiss-Prot


Match Name	E-value	Identity	Description
LDHA_HORVU	8.5e-129	69.83	L-lactate dehydrogenase A OS=Hordeum vulgare PE=1 SV=1	[more]
LDHB_HORVU	1.1e-125	67.84	L-lactate dehydrogenase B (Fragment) OS=Hordeum vulgare PE=1 SV=1	[more]
LDH_MAIZE	2.9e-121	66.48	L-lactate dehydrogenase OS=Zea mays PE=2 SV=1	[more]
LDHA_MOUSE	5.7e-93	55.63	L-lactate dehydrogenase A chain OS=Mus musculus GN=Ldha PE=1 SV=3	[more]
LDHA_RAT	4.8e-92	55.31	L-lactate dehydrogenase A chain OS=Rattus norvegicus GN=Ldha PE=1 SV=1	[more]

BLAST of Lsi09G004170 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KKU9_CUCSA	2.0e-177	93.43	L-lactate dehydrogenase OS=Cucumis sativus GN=Csa_5G161920 PE=3 SV=1	[more]
A0A067KUG8_JATCU	1.7e-168	86.00	L-lactate dehydrogenase OS=Jatropha curcas GN=JCGZ_03382 PE=3 SV=1	[more]
A0A0D2UW00_GOSRA	6.0e-166	85.14	L-lactate dehydrogenase OS=Gossypium raimondii GN=B456_009G297700 PE=3 SV=1	[more]
A0A151TJG6_CAJCA	7.9e-166	85.43	L-lactate dehydrogenase OS=Cajanus cajan GN=KK1_013505 PE=3 SV=1	[more]
A0A0B0P6A6_GOSAR	1.3e-165	85.14	L-lactate dehydrogenase OS=Gossypium arboreum GN=F383_25947 PE=3 SV=1	[more]

BLAST of Lsi09G004170 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G17260.1	1.1e-155	76.77	Lactate/malate dehydrogenase family protein	[more]

BLAST of Lsi09G004170 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659073513\|ref\|XP_008437100.1\|	2.2e-185	96.86	PREDICTED: L-lactate dehydrogenase B [Cucumis melo]	[more]
gi\|778700018\|ref\|XP_004151663.2\|	1.2e-183	95.71	PREDICTED: LOW QUALITY PROTEIN: L-lactate dehydrogenase A-like [Cucumis sativus]	[more]
gi\|778700014\|ref\|XP_011654796.1\|	2.9e-177	93.43	PREDICTED: L-lactate dehydrogenase B-like [Cucumis sativus]	[more]
gi\|802581859\|ref\|XP_012069951.1\|	2.4e-168	86.00	PREDICTED: L-lactate dehydrogenase B [Jatropha curcas]	[more]
gi\|823228917\|ref\|XP_012447203.1\|	8.7e-166	85.14	PREDICTED: L-lactate dehydrogenase B-like [Gossypium raimondii]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process
GO:0019752	carboxylic acid metabolic process
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0004459	L-lactate dehydrogenase activity
GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0016491	oxidoreductase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **INTERPRO**
Term	Definition
IPR022383	Lactate/malate_DH_C
IPR018177	L-lactate_DH_AS
IPR016040	NAD(P)-bd_dom
IPR015955	Lactate_DH/Glyco_Ohase_4_C
IPR011304	L-lactate_DH
IPR001557	L-lactate/malate_DH
IPR001236	Lactate/malate_DH_N

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006534	cysteine metabolic process
biological_process	GO:0006094	gluconeogenesis
biological_process	GO:0006096	glycolytic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0019752	carboxylic acid metabolic process
biological_process	GO:0009737	response to abscisic acid
biological_process	GO:0009651	response to salt stress
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004459	L-lactate dehydrogenase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G004170.1	Lsi09G004170.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001236	Lactate/malate dehydrogenase, N-terminal	PFAM	PF00056	Ldh_1_N	coord: 39..177 score: 3.8
IPR001557	L-lactate/malate dehydrogenase	PRINTS	PR00086	LLDHDRGNASE	coord: 39..63 score: 8.9E-54coord: 64..88 score: 8.9E-54coord: 205..218 score: 8.9E-54coord: 175..193 score: 8.9E-54coord: 151..171 score: 8.9
IPR001557	L-lactate/malate dehydrogenase	PIR	PIRSF000102	Lac_mal_DH	coord: 34..350 score: 2.0E
IPR001557	L-lactate/malate dehydrogenase	PANTHER	PTHR11540	MALATE AND LACTATE DEHYDROGENASE	coord: 10..348 score: 6.7E
IPR011304	L-lactate dehydrogenase	HAMAP	MF_00488	Lactate_dehydrog	coord: 37..349 score: 37
IPR011304	L-lactate dehydrogenase	TIGRFAMs	TIGR01771	TIGR01771	coord: 42..341 score: 5.2E
IPR015955	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal	GENE3D	G3DSA:3.90.110.10		coord: 180..348 score: 6.6
IPR015955	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal	unknown	SSF56327	LDH C-terminal domain-like	coord: 178..346 score: 1.73
IPR016040	NAD(P)-binding domain	GENE3D	G3DSA:3.40.50.720		coord: 29..179 score: 1.1
IPR016040	NAD(P)-binding domain	unknown	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 24..177 score: 3.47
IPR018177	L-lactate dehydrogenase, active site	PROSITE	PS00064	L_LDH	coord: 207..213 scor
IPR022383	Lactate/malate dehydrogenase, C-terminal	PFAM	PF02866	Ldh_1_C	coord: 180..343 score: 1.9
None	No IPR available	PANTHER	PTHR11540:SF3	L-LACTATE DEHYDROGENASE	coord: 10..348 score: 6.7E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi09G004170	Csa5G161910	Cucumber (Chinese Long) v2	culsiB322
Lsi09G004170	CSPI05G06740	Wild cucumber (PI 183967)	cpilsiB322
Lsi09G004170	Cucsa.301890	Cucumber (Gy14) v1	cgylsiB487
Lsi09G004170	CmaCh15G011280	Cucurbita maxima (Rimu)	cmalsiB267
Lsi09G004170	CmaCh02G015450	Cucurbita maxima (Rimu)	cmalsiB537
Lsi09G004170	CmoCh02G015870	Cucurbita moschata (Rifu)	cmolsiB522
Lsi09G004170	CmoCh15G011870	Cucurbita moschata (Rifu)	cmolsiB255
Lsi09G004170	Cla011577	Watermelon (97103) v1	lsiwmB027
Lsi09G004170	MELO3C005539	Melon (DHL92) v3.5.1	lsimeB012
Lsi09G004170	ClCG01G003970	Watermelon (Charleston Gray)	lsiwcgB008
Lsi09G004170	MELO3C005539.2	Melon (DHL92) v3.6.1	lsimedB012
Lsi09G004170	Cla97C01G004140	Watermelon (97103) v2	lsiwmbB007
Lsi09G004170	Bhi12G000410	Wax gourd	lsiwgoB010
Lsi09G004170	Cp4.1LG05g02440	Cucurbita pepo (Zucchini)	cpelsiB580
Lsi09G004170	Cp4.1LG13g02350	Cucurbita pepo (Zucchini)	cpelsiB131
Lsi09G004170	CsGy5G004080	Cucumber (Gy14) v2	cgyblsiB294
Lsi09G004170	Carg23210	Silver-seed gourd	carlsiB493
Lsi09G004170	Carg02443	Silver-seed gourd	carlsiB834
Lsi09G004170	CsaV3_5G004150	Cucumber (Chinese Long) v3	cuclsiB345

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi09G004170	Cucumber (Chinese Long) v3	cuclsiB172
Lsi09G004170	Bottle gourd (USVL1VR-Ls)	lsilsiB019
Lsi09G004170	Cucumber (Gy14) v1	cgylsiB526
Lsi09G004170	Wild cucumber (PI 183967)	cpilsiB154
Lsi09G004170	Cucumber (Chinese Long) v2	culsiB155
Lsi09G004170	Watermelon (Charleston Gray)	lsiwcgB007
Lsi09G004170	Watermelon (97103) v1	lsiwmB008