Lsi09G004170.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi09G004170.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	L-lactate dehydrogenase
Location	chr09 : 4441686 .. 4444057 (-)
Sequence length	1053

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGAAGAGTTCATCAACATCTTCTTTAGGTCCAGGCGGCCTTGACCTTTCTCAAGCCTTCTTCAAACCAATCCGCGACGCCTCTCCCCCATCGACTTCAAAGCGCAACTCCAAAATCTCCGTCATCGGCGCCGGGAACGTCGGCATGGCCATCGCTCAGACCATCCTCACTCAAGATCTTGTCGAAGAACTCGTCCTCGTCGACGCCAAGCCCGACAAACTCCGCGGCGAAATGCTCGATCTCCAACACGCTGCCGCCTTCCTCCCCCGAACCAAAATCAGCGCCTCCACCGATTACTCCATTACCGCTGGATCCGATCTATGTATCGTCACCGCTGGAGCTCGCCAGATCGCTGGTGAATCTAGGTTGAATCTGCTGCAGAGGAACGTTGCTCTGTTTCAGAAGATCGTGCCGCCGCTGGTTCAGTTCTCTCCCGAGACGATTCTTTTGATTGTTTCGAATCCGGTGGATGTTTTGACTTATGTTGCTTGGAAGCTCTCTGGTTTTCCGTCTAATCGAGTGATTGGATCCGGAACGAATCTGGATTCGTCTAGGTTTCGGTTTCTCATCGCCGATCATCTGGACGTCAATGCTCAGGATGTTCAGGTCAGTTTTCTGATTGACATTTCTTCTACCTTCTTCAATCAATCAGTGTTTATTTGTTAATTTGGTGTTGCTTTTTGAAGAAATTTCAATTTAGGGTTTGAAACCTTTCGATTTTTTCGTTTTTGGTTGATTACTTGATTAGTATCAAATGTGGCTGATAATTTTAGGGAAAATTTCGAACATTTCCCTAGAATTTGGAAGTTGGCAACTCGCCAACAAATCACAATAACTATTTGAAATATCTATCCGGAATATATGTTATGTGGATTAAATCTTATTTTCAACTTTCATATTTATTTAATTTTGATTTCTTAACTTTTTTGATGTTTATTTTAATCATTGAATTTTTTAATAATAAAACCTAAATTTTTTTTGGTTAAATTATAAGTTTAGTACTTTCATTTTTATGTTTGTGATTAACACGTCTTTGAACATTCAATTATATTTCTAATAGGTTTTTAACCTTTAAAATCATCGAATAGATCATTGAACTTTCAGATTTATGTCTAATAGATTATAATATAAATATATAATTTTAAAATGAACAAATCAATTAGCCATAATATAAAATTGATTCAATTACAAATTTAGTTCATAAACTTTGTGACTTCTGTCTATTTGACCTTTGAACTTTGAAATTACAAACTCAGTTTGAATTTTTCTTATATATACTTTTAAATTTCACGAATCTGCTAGACATAAAACTGAAAATTTGTAGTTTAATTAGACATAAAATTCAAATTTATATCAAATAGATTGAAATTTTATGAGCCTATTAGACACAAAAGTAAAAGTTTAGAGATCTATTAGACAATTTCAAAAAATCTTGAATAGGTTTAAGAACTATTTTACTTAAAACTAAATATGTAAGTTTATTAAACACAAAATCTAAAGTTTAGAGAGTTATTAGACACTTTTTAAAGTTGATAAATCTATTAAAATTGAGAGACTTATTTACCACAAACCAAAAAGTACATAGTCTAAACTTAGTAATTTAACTAAAAATGTAAAAATAACCAATTTTCTACCTAATATTTCTTCCCCAATACTCACCTCTGTTAAACTTGTTAATAGCAAAACAAAGATAAAGGACAATTATAGTTATTACTTTTAATGTTGAAGGAGAAAAATTAGAAAAAGAAGAAACAATAAATTCAAAGAATGAAAAAATAAATTCAAAGTTGACCCAAGTAGAATTCAAACCATCATATAGGTGCCTAACCTATGCATCAAACTATAGATGAATTTCTATAAATCAAACTAGCATTGATATTTAAAAGATGAAGCTAAAAAAAAGAGGATGATATATATGTTTGAAGGCATACATAGTGGGAGAGCATGGCGACAGCTCGGTGGCGCTATGGTCAAGCATAAGTGTGGGCGGGGTGCCAATACTGAGCTTTCTAAAGAATCAACAAATTGCCTACGAAAAAGAAACATTAGAGAAAATTCACAAAGAGGTCGTAGGCGGGGCCTATGAAGTCATAAGCCTGAAGGGCTACACATCATGGGCGATTGGGTACTCCGTGGCGAGCTTGGCTCGGTCGCTCCTTCGTGACCAACGGAGGATCCACCCCGTCTCTGTCCTTGCAAAAGGGTTCTACGGCATTGATGGTGGCGAGTTGTTCCTCAGCCTGCCTGCACAGCTCGGGAGGGGCGGAGTATTGGGGATCACCAATGTGCATCTAACGGCGGAGGAGTCGAGACGTTTGAGGGACTCGGCGAACACCATCTTGCAGGTGCAGACTGAATTGGGGATCTGA

mRNA sequence

ATGCAGAAGAGTTCATCAACATCTTCTTTAGGTCCAGGCGGCCTTGACCTTTCTCAAGCCTTCTTCAAACCAATCCGCGACGCCTCTCCCCCATCGACTTCAAAGCGCAACTCCAAAATCTCCGTCATCGGCGCCGGGAACGTCGGCATGGCCATCGCTCAGACCATCCTCACTCAAGATCTTGTCGAAGAACTCGTCCTCGTCGACGCCAAGCCCGACAAACTCCGCGGCGAAATGCTCGATCTCCAACACGCTGCCGCCTTCCTCCCCCGAACCAAAATCAGCGCCTCCACCGATTACTCCATTACCGCTGGATCCGATCTATGTATCGTCACCGCTGGAGCTCGCCAGATCGCTGGTGAATCTAGGTTGAATCTGCTGCAGAGGAACGTTGCTCTGTTTCAGAAGATCGTGCCGCCGCTGGTTCAGTTCTCTCCCGAGACGATTCTTTTGATTGTTTCGAATCCGGTGGATGTTTTGACTTATGTTGCTTGGAAGCTCTCTGGTTTTCCGTCTAATCGAGTGATTGGATCCGGAACGAATCTGGATTCGTCTAGGTTTCGGTTTCTCATCGCCGATCATCTGGACGTCAATGCTCAGGATGTTCAGGCATACATAGTGGGAGAGCATGGCGACAGCTCGGTGGCGCTATGGTCAAGCATAAGTGTGGGCGGGGTGCCAATACTGAGCTTTCTAAAGAATCAACAAATTGCCTACGAAAAAGAAACATTAGAGAAAATTCACAAAGAGGTCGTAGGCGGGGCCTATGAAGTCATAAGCCTGAAGGGCTACACATCATGGGCGATTGGGTACTCCGTGGCGAGCTTGGCTCGGTCGCTCCTTCGTGACCAACGGAGGATCCACCCCGTCTCTGTCCTTGCAAAAGGGTTCTACGGCATTGATGGTGGCGAGTTGTTCCTCAGCCTGCCTGCACAGCTCGGGAGGGGCGGAGTATTGGGGATCACCAATGTGCATCTAACGGCGGAGGAGTCGAGACGTTTGAGGGACTCGGCGAACACCATCTTGCAGGTGCAGACTGAATTGGGGATCTGA

Coding sequence (CDS)

ATGCAGAAGAGTTCATCAACATCTTCTTTAGGTCCAGGCGGCCTTGACCTTTCTCAAGCCTTCTTCAAACCAATCCGCGACGCCTCTCCCCCATCGACTTCAAAGCGCAACTCCAAAATCTCCGTCATCGGCGCCGGGAACGTCGGCATGGCCATCGCTCAGACCATCCTCACTCAAGATCTTGTCGAAGAACTCGTCCTCGTCGACGCCAAGCCCGACAAACTCCGCGGCGAAATGCTCGATCTCCAACACGCTGCCGCCTTCCTCCCCCGAACCAAAATCAGCGCCTCCACCGATTACTCCATTACCGCTGGATCCGATCTATGTATCGTCACCGCTGGAGCTCGCCAGATCGCTGGTGAATCTAGGTTGAATCTGCTGCAGAGGAACGTTGCTCTGTTTCAGAAGATCGTGCCGCCGCTGGTTCAGTTCTCTCCCGAGACGATTCTTTTGATTGTTTCGAATCCGGTGGATGTTTTGACTTATGTTGCTTGGAAGCTCTCTGGTTTTCCGTCTAATCGAGTGATTGGATCCGGAACGAATCTGGATTCGTCTAGGTTTCGGTTTCTCATCGCCGATCATCTGGACGTCAATGCTCAGGATGTTCAGGCATACATAGTGGGAGAGCATGGCGACAGCTCGGTGGCGCTATGGTCAAGCATAAGTGTGGGCGGGGTGCCAATACTGAGCTTTCTAAAGAATCAACAAATTGCCTACGAAAAAGAAACATTAGAGAAAATTCACAAAGAGGTCGTAGGCGGGGCCTATGAAGTCATAAGCCTGAAGGGCTACACATCATGGGCGATTGGGTACTCCGTGGCGAGCTTGGCTCGGTCGCTCCTTCGTGACCAACGGAGGATCCACCCCGTCTCTGTCCTTGCAAAAGGGTTCTACGGCATTGATGGTGGCGAGTTGTTCCTCAGCCTGCCTGCACAGCTCGGGAGGGGCGGAGTATTGGGGATCACCAATGTGCATCTAACGGCGGAGGAGTCGAGACGTTTGAGGGACTCGGCGAACACCATCTTGCAGGTGCAGACTGAATTGGGGATCTGA

Protein sequence

MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQDLVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYEKETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGIDGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI

Homology

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BLAST of Lsi09G004170.1 vs. Swiss-Prot
Match: LDHA_HORVU (L-lactate dehydrogenase A OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 8.5e-129
Identity = 243/348 (69.83%), Postives = 287/348 (82.47%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASP--PSTS----KRNSKISVIGAGNVGMAIAQ 60
           M K+SS S LG      S  FF+P+ D  P  P++S    +R +KISVIGAGNVGMAIAQ
Sbjct: 1   MHKASSLSELGFDAGGASSGFFRPVADGCPATPTSSAVPHRRLTKISVIGAGNVGMAIAQ 60

Query: 61  TILTQDLVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAG 120
           TILTQ+L +E+ LVDA PDKLRGE LDLQHAAAFLPR +IS  TD ++T  SDL IVTAG
Sbjct: 61  TILTQNLADEIALVDALPDKLRGEALDLQHAAAFLPRVRIS-GTDAAVTKNSDLVIVTAG 120

Query: 121 ARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNR 180
           ARQI GE+RLNLLQRNVAL++KIVPP+ + SP+ +LL+VSNPVDVLTYVAWKLSGFP++R
Sbjct: 121 ARQIPGETRLNLLQRNVALYRKIVPPVAEHSPDALLLVVSNPVDVLTYVAWKLSGFPASR 180

Query: 181 VIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKN 240
           VIGSGTNLDSSRFRFLIA+HLDVNAQDVQAY+VGEHGDSSVA+WSSISVGG+P    L++
Sbjct: 181 VIGSGTNLDSSRFRFLIAEHLDVNAQDVQAYMVGEHGDSSVAIWSSISVGGMPAFKSLRD 240

Query: 241 QQIAYEKETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLA 300
              ++++  LE I + VVGGAYEVI LKGYTSWAIGYSVASLA SLLRDQRR+HPVSVLA
Sbjct: 241 SHRSFDEAALEGIRRAVVGGAYEVIGLKGYTSWAIGYSVASLAASLLRDQRRVHPVSVLA 300

Query: 301 KGFYGI-DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTI 342
            GF+GI DG E+FLSLPA+LGRGG+LG+  + LT  E+ +LR SA T+
Sbjct: 301 SGFHGISDGHEVFLSLPARLGRGGILGVAEMDLTEAEAAQLRRSAKTL 347

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BLAST of Lsi09G004170.1 vs. Swiss-Prot
Match: LDHB_HORVU (L-lactate dehydrogenase B (Fragment) OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.1e-125
Identity = 232/342 (67.84%), Postives = 280/342 (81.87%), Query Frame = 1


Query: 16  DLSQAFFKPIRDASP------PSTSKRNSKISVIGAGNVGMAIAQTILTQDLVEELVLVD 75
           D S  FF+ + D  P       +  +R +K+SVIGAGNVGMAIAQTILTQ+L +E+ LVD
Sbjct: 3   DASSGFFRAVADGCPITHTSCSAPHRRLTKVSVIGAGNVGMAIAQTILTQNLADEIALVD 62

Query: 76  AKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAGESRLNLLQR 135
           A PDKLRGE LDLQHAAAFLPR +I + TD ++T  SDL +VTAGARQI GE+RLNLLQR
Sbjct: 63  ALPDKLRGEALDLQHAAAFLPRVRIVSGTDAAVTKNSDLIVVTAGARQIPGETRLNLLQR 122

Query: 136 NVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGTNLDSSRFRF 195
           NVAL++KIVPP+ + SP+ +LL+VSNPVDVLTYVAWKLSGFP++RVIGSGTNLDSSRFRF
Sbjct: 123 NVALYRKIVPPVAEHSPDALLLVVSNPVDVLTYVAWKLSGFPASRVIGSGTNLDSSRFRF 182

Query: 196 LIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYEKETLEKIHK 255
           L+A+HLDV+AQDVQAY+VGEHGDSSVA+WSSISVGG+P L  L++   ++++  LE I +
Sbjct: 183 LVAEHLDVSAQDVQAYMVGEHGDSSVAIWSSISVGGMPALKSLRDSHRSFDEAALEGIRR 242

Query: 256 EVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI-DGGELFLS 315
            VVGGAYEVI LKGYTSWAIGYSVASLA SLLRDQRR+HPVSVLA GF+GI DG E+FLS
Sbjct: 243 AVVGGAYEVIGLKGYTSWAIGYSVASLATSLLRDQRRVHPVSVLAAGFHGISDGHEVFLS 302

Query: 316 LPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           LPA+LGR GVLG+  + LT  E+ +LR SA T+ +    LG+
Sbjct: 303 LPARLGRAGVLGVAEMDLTEAEAAQLRRSAKTLWENCQLLGL 344

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BLAST of Lsi09G004170.1 vs. Swiss-Prot
Match: LDH_MAIZE (L-lactate dehydrogenase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 2.9e-121
Identity = 236/355 (66.48%), Postives = 284/355 (80.00%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIR-DASPPSTS---KRNSKISVIGAGNVGMAIAQTI 60
           M+K++S S LG    D S  FF+P+  D+S P++    +R +K+SVIGAGNVGMAIAQTI
Sbjct: 1   MKKATSLSELGFDAGDASSGFFRPVSGDSSTPTSQHHRRRLTKVSVIGAGNVGMAIAQTI 60

Query: 61  LTQDLVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGAR 120
           LT+DL +E+ LVDA PDKLRGEMLDLQHAAAFLPRT++ + TD S+T GSDL IVTAGAR
Sbjct: 61  LTRDLADEIALVDAVPDKLRGEMLDLQHAAAFLPRTRLVSGTDMSVTRGSDLVIVTAGAR 120

Query: 121 QIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVI 180
           QI GE+RL+LLQRNVALF+KIVPPL + S + +LL+VSNPVDVLTYVAWKLSGFP++RVI
Sbjct: 121 QIQGETRLDLLQRNVALFRKIVPPLAEQSHDALLLVVSNPVDVLTYVAWKLSGFPASRVI 180

Query: 181 GSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQ 240
           GSGTNLDSSRFRFL+A+HLDVNAQDVQAY+VGEHGDSSVA+WSS+SV G+P+L  L+   
Sbjct: 181 GSGTNLDSSRFRFLLAEHLDVNAQDVQAYMVGEHGDSSVAVWSSVSVAGMPVLKSLQESH 240

Query: 241 IAYEKETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKG 300
             +++E LE I + VV  AYEVISLKGYTSWAIGYSVASLA SLLRDQRRIHPVSVLA+G
Sbjct: 241 RCFDEEALEGIRRAVVDSAYEVISLKGYTSWAIGYSVASLAASLLRDQRRIHPVSVLARG 300

Query: 301 FYGI-DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           F+GI DG     + P +  R    G   + LT EE++RLR SA TI +    LG+
Sbjct: 301 FHGIPDGTTSSSACPPRRPRRRP-GRREMELTEEEAKRLRRSAKTIWENCQLLGL 354

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BLAST of Lsi09G004170.1 vs. Swiss-Prot
Match: LDHA_MOUSE (L-lactate dehydrogenase A chain OS=Mus musculus GN=Ldha PE=1 SV=3)

HSP 1 Score: 342.4 bits (877), Expect = 5.7e-93
Identity = 173/311 (55.63%), Postives = 229/311 (73.63%), Query Frame = 1


Query: 38  SKISVIGAGNVGMAIAQTILTQDLVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISAS 97
           +KI+V+G G VGMA A +IL +DL +EL LVD   DKL+GEM+DLQH + FL   KI +S
Sbjct: 21  NKITVVGVGAVGMACAISILMKDLADELALVDVMEDKLKGEMMDLQHGSLFLKTPKIVSS 80

Query: 98  TDYSITAGSDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPV 157
            DY +TA S L I+TAGARQ  GESRLNL+QRNV +F+ I+P +V++SP   LLIVSNPV
Sbjct: 81  KDYCVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNIVKYSPHCKLLIVSNPV 140

Query: 158 DVLTYVAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVAL 217
           D+LTYVAWK+SGFP NRVIGSG NLDS+RFR+L+ + L V+A     +++GEHGDSSV +
Sbjct: 141 DILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHALSCHGWVLGEHGDSSVPV 200

Query: 218 WSSISVGGVPILSFLKNQQIAYEKETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLA 277
           WS ++V GV + S         +KE  +++HK+VV  AYEVI LKGYTSWAIG SVA LA
Sbjct: 201 WSGVNVAGVSLKSLNPELGTDADKEQWKEVHKQVVDSAYEVIKLKGYTSWAIGLSVADLA 260

Query: 278 RSLLRDQRRIHPVSVLAKGFYGIDGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDS 337
            S++++ RR+HP+S + KG YGI+  ++FLS+P  LG+ G+  +  V LT EE  RL+ S
Sbjct: 261 ESIMKNLRRVHPISTMIKGLYGIN-EDVFLSVPCILGQNGISDVVKVTLTPEEEARLKKS 320

Query: 338 ANTILQVQTEL 349
           A+T+  +Q EL
Sbjct: 321 ADTLWGIQKEL 330

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BLAST of Lsi09G004170.1 vs. Swiss-Prot
Match: LDHA_RAT (L-lactate dehydrogenase A chain OS=Rattus norvegicus GN=Ldha PE=1 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 4.8e-92
Identity = 172/311 (55.31%), Postives = 228/311 (73.31%), Query Frame = 1


Query: 38  SKISVIGAGNVGMAIAQTILTQDLVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISAS 97
           +KI+V+G G VGMA A +IL +DL +EL LVD   DKL+GEM+DLQH + FL   KI +S
Sbjct: 21  NKITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGSLFLKTPKIVSS 80

Query: 98  TDYSITAGSDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPV 157
            DYS+TA S L I+TAGARQ  GESRLNL+QRNV +F+ I+P +V++SP+  LLIVSNPV
Sbjct: 81  KDYSVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNVVKYSPQCKLLIVSNPV 140

Query: 158 DVLTYVAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVAL 217
           D+LTYVAWK+SGFP NRVIGSG NLDS+RFR+L+ + L V+      +++GEHGDSSV +
Sbjct: 141 DILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHPLSCHGWVLGEHGDSSVPV 200

Query: 218 WSSISVGGVPILSFLKNQQIAYEKETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLA 277
           WS ++V GV + S         +KE  + +HK+VV  AYEVI LKGYTSWAIG SVA LA
Sbjct: 201 WSGVNVAGVSLKSLNPQLGTDADKEQWKDVHKQVVDSAYEVIKLKGYTSWAIGLSVADLA 260

Query: 278 RSLLRDQRRIHPVSVLAKGFYGIDGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDS 337
            S++++ RR+HP+S + KG YGI   ++FLS+P  LG+ G+  +  V LT +E  RL+ S
Sbjct: 261 ESIMKNLRRVHPISTMIKGLYGIK-EDVFLSVPCILGQNGISDVVKVTLTPDEEARLKKS 320

Query: 338 ANTILQVQTEL 349
           A+T+  +Q EL
Sbjct: 321 ADTLWGIQKEL 330

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BLAST of Lsi09G004170.1 vs. TrEMBL
Match: A0A0A0KKU9_CUCSA (L-lactate dehydrogenase OS=Cucumis sativus GN=Csa_5G161920 PE=3 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 2.0e-177
Identity = 327/350 (93.43%), Postives = 337/350 (96.29%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS STSSLGPGGLDLSQAFFKPIRDASP STS  N+KISVIG GNVGMAIAQTILTQD
Sbjct: 63  MQKSPSTSSLGPGGLDLSQAFFKPIRDASPLSTSNCNTKISVIGTGNVGMAIAQTILTQD 122

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LV+ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITA SDLCIVTAGARQIAG
Sbjct: 123 LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITARSDLCIVTAGARQIAG 182

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNV LFQKIVP LVQFSPETILLIVSNPVDVLTYVAWKLSGF SNRVIGSGT
Sbjct: 183 ESRLNLLQRNVTLFQKIVPLLVQFSPETILLIVSNPVDVLTYVAWKLSGFSSNRVIGSGT 242

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFLKNQQI YE
Sbjct: 243 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 302

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEV+GGAYEVI+LKGYTSWAIGYSVASLARS+LRDQRRIHPVSV+AKGFYGI
Sbjct: 303 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVIAKGFYGI 362

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           +GG+LFLSLPAQLGRGGVLGITNVHLT EES+RLRDSANTIL VQ ELGI
Sbjct: 363 EGGDLFLSLPAQLGRGGVLGITNVHLTTEESKRLRDSANTILHVQAELGI 412

[Close]

BLAST of Lsi09G004170.1 vs. TrEMBL
Match: A0A067KUG8_JATCU (L-lactate dehydrogenase OS=Jatropha curcas GN=JCGZ_03382 PE=3 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 1.7e-168
Identity = 301/350 (86.00%), Postives = 331/350 (94.57%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS S S+LGPGGLDL+QAFFKPI++ +PPS +KR++KISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASTLGPGGLDLTQAFFKPIQNTAPPSPTKRHTKISVIGTGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L +EL LVDAKPDKLRGEMLDLQHAAAFLPRTKI ASTDYS+T GSDLCIVTAGARQI G
Sbjct: 61  LADELALVDAKPDKLRGEMLDLQHAAAFLPRTKIIASTDYSVTVGSDLCIVTAGARQIPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF+KI+PPLV +SPE+IL+IVSNPVDVLTY+AWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFEKIIPPLVNYSPESILMIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFL+ QQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLEKQQIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLE IHK V+  AYEVI+LKGYTSWAIGYS A+LARSLLRDQR+IHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKAVIDSAYEVINLKGYTSWAIGYSAANLARSLLRDQRKIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGGE+FLSLPAQLGRGGVLG+TNVHLT EE+RRLR+SA T+L+VQ++LGI
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNVHLTEEEARRLRESAKTLLEVQSQLGI 350

[Close]

BLAST of Lsi09G004170.1 vs. TrEMBL
Match: A0A0D2UW00_GOSRA (L-lactate dehydrogenase OS=Gossypium raimondii GN=B456_009G297700 PE=3 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 6.0e-166
Identity = 298/350 (85.14%), Postives = 328/350 (93.71%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDL+QAFFKPI++  PPS++KR++KISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASSLGPGGLDLAQAFFKPIQNCDPPSSTKRHTKISVIGVGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L +EL LVDAK DKLRGEMLDLQHAAAFLPRTKI+AS DYS+TAGSDLCIVTAGARQ  G
Sbjct: 61  LADELALVDAKSDKLRGEMLDLQHAAAFLPRTKINASVDYSVTAGSDLCIVTAGARQNPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF  I+PPL ++SP++ILLIVSNPVDVLTYV+WKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFSNIIPPLAKYSPDSILLIVSNPVDVLTYVSWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSIS+GGVP+LSFLKNQQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISIGGVPVLSFLKNQQIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           K+TLE IHK VV  AYEVISLKGYTSWAIGYSVA+LARSLLRDQR+IHPVSVLAKGFYGI
Sbjct: 241 KQTLENIHKAVVESAYEVISLKGYTSWAIGYSVANLARSLLRDQRKIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGGE+FLSLPAQLGRGGVLG+TN+HLT EE +RLR SA TIL+VQ++LG+
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNIHLTDEEVQRLRKSAETILEVQSQLGL 350

[Close]

BLAST of Lsi09G004170.1 vs. TrEMBL
Match: A0A151TJG6_CAJCA (L-lactate dehydrogenase OS=Cajanus cajan GN=KK1_013505 PE=3 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 7.9e-166
Identity = 299/350 (85.43%), Postives = 330/350 (94.29%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           M KS S+S+LGPGGLDL+QAFFK I +A+P S +KR++KISV+GAGNVGMAIAQTILTQD
Sbjct: 1   MHKSGSSSALGPGGLDLTQAFFKSIMNAAPSSPTKRHNKISVVGAGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L +EL LVDA P+KLRGEMLDLQHAAAFLPRTKI ASTDY++TAGSDLCIVTAGARQI G
Sbjct: 61  LTDELALVDAIPEKLRGEMLDLQHAAAFLPRTKIHASTDYAVTAGSDLCIVTAGARQING 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNV LFQKI+PPLV  SP+TILLIVSNPVDVL+YVAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVTLFQKIIPPLVGHSPDTILLIVSNPVDVLSYVAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVP+LSFL+ Q+IAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPVLSFLEKQEIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           +E LEKIHKEV+ GAYEVISLKGYTSWAIGYSVA+LARS+LR+QRR+HPVSVLAKGFYGI
Sbjct: 241 REMLEKIHKEVIQGAYEVISLKGYTSWAIGYSVANLARSILRNQRRVHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGGE+FLSLPAQLGRGGVLG+TNVHLT EES+RLRDSANTIL+VQ +L I
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNVHLTEEESQRLRDSANTILEVQNQLAI 350

[Close]

BLAST of Lsi09G004170.1 vs. TrEMBL
Match: A0A0B0P6A6_GOSAR (L-lactate dehydrogenase OS=Gossypium arboreum GN=F383_25947 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 1.3e-165
Identity = 298/350 (85.14%), Postives = 327/350 (93.43%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDL+QAFFKPI +  PPS++KR++KISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASSLGPGGLDLAQAFFKPILNTDPPSSTKRHTKISVIGVGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L +EL LVDAK DKLRGEMLDLQHAAAFLPRTKI+AS DYS+TAGSDLCIVTAGARQ  G
Sbjct: 61  LADELALVDAKSDKLRGEMLDLQHAAAFLPRTKINASVDYSVTAGSDLCIVTAGARQNPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF  I+PPL ++SP++ILLIVSNPVDVLTYV+WKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFSNIIPPLAKYSPDSILLIVSNPVDVLTYVSWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSIS+GGVP+LSFLKNQQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISIGGVPVLSFLKNQQIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           K+TLE IHK VV  AYEVISLKGYTSWAIGYSVA+LARSLLRDQR+IHPVSVLAKGFYGI
Sbjct: 241 KQTLENIHKAVVESAYEVISLKGYTSWAIGYSVANLARSLLRDQRKIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGGE+FLSLPAQLGRGGVLG+TN+HLT EE +RLR SA TIL+VQ++LG+
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNIHLTDEEVQRLRKSAETILEVQSQLGL 350

[Close]

BLAST of Lsi09G004170.1 vs. TAIR10
Match: AT4G17260.1 (AT4G17260.1 Lactate/malate dehydrogenase family protein)

HSP 1 Score: 546.6 bits (1407), Expect = 1.1e-155
Identity = 271/353 (76.77%), Postives = 317/353 (89.80%), Query Frame = 1


Query: 1   MQKSSSTSSL---GPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTIL 60
           M+K++STSSL   GP GLDL+ AFFKPI ++ P   S R +K+SV+G GNVGMAIAQTIL
Sbjct: 1   MEKNASTSSLKDLGPSGLDLTSAFFKPIHNSDPSLPSNRRTKVSVVGVGNVGMAIAQTIL 60

Query: 61  TQDLVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQ 120
           TQDL +E+ LVDAKPDKLRGEMLDLQHAAAFLPRTKI+AS DY +TAGSDLCIVTAGARQ
Sbjct: 61  TQDLADEIALVDAKPDKLRGEMLDLQHAAAFLPRTKITASVDYEVTAGSDLCIVTAGARQ 120

Query: 121 IAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIG 180
             GESRLNLLQRNVALF+ I+PPL + SP++IL+IVSNPVDVLTYVAWKLSGFP NRV+G
Sbjct: 121 NPGESRLNLLQRNVALFRHIIPPLAKASPDSILIIVSNPVDVLTYVAWKLSGFPVNRVLG 180

Query: 181 SGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQI 240
           SGTNLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGG+P+LSFL+  QI
Sbjct: 181 SGTNLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGIPVLSFLEKNQI 240

Query: 241 AYEKETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGF 300
           AYEK+TLE IH+ VVG AYEVI LKGYTSWAIGYSVA+LAR++LRDQR+IHPV+VLA+GF
Sbjct: 241 AYEKQTLEDIHQAVVGSAYEVIGLKGYTSWAIGYSVANLARTILRDQRKIHPVTVLARGF 300

Query: 301 YGIDGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           YG+DGG++FLSLPA LGR GV+ +TNVH+T EE+ +L+ SA TIL++Q++LG+
Sbjct: 301 YGVDGGDVFLSLPALLGRNGVVAVTNVHMTDEEAEKLQKSAKTILEMQSQLGL 353

[Close]

BLAST of Lsi09G004170.1 vs. NCBI nr
Match: gi|659073513|ref|XP_008437100.1| (PREDICTED: L-lactate dehydrogenase B [Cucumis melo])

HSP 1 Score: 656.8 bits (1693), Expect = 2.2e-185
Identity = 339/350 (96.86%), Postives = 347/350 (99.14%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS STSSLGPGGLDLSQAFFKPIRDASPPSTS RN+KISVIGAGNVGMAIAQTILTQD
Sbjct: 83  MQKSPSTSSLGPGGLDLSQAFFKPIRDASPPSTSNRNTKISVIGAGNVGMAIAQTILTQD 142

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LV+ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYS TAGS+LCIVTAGARQIAG
Sbjct: 143 LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSTTAGSNLCIVTAGARQIAG 202

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT
Sbjct: 203 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 262

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE
Sbjct: 263 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 322

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEV+GGAYEVI+LKGYTSWAIGYSVASLARS+LRDQRRIHPVSVLAKGFYGI
Sbjct: 323 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 382

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGG+LFLSLPAQLGRGGVLGITNVHLTAEES+RLRDSANTILQVQTELGI
Sbjct: 383 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 432

[Close]

BLAST of Lsi09G004170.1 vs. NCBI nr
Match: gi|778700018|ref|XP_004151663.2| (PREDICTED: LOW QUALITY PROTEIN: L-lactate dehydrogenase A-like [Cucumis sativus])

HSP 1 Score: 651.0 bits (1678), Expect = 1.2e-183
Identity = 335/350 (95.71%), Postives = 345/350 (98.57%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKSSS SSLGPGGLDLSQAFFKPIRDASPPST+ RN+KISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LV+ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVT GARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTY+AWKLSGFPSNRVI SGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIXSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NL+SSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQI YE
Sbjct: 181 NLNSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEV+GGAYEVI+LKGYTSWAIGYSVASLARS+LRDQRRIHPVSVLAKGFYGI
Sbjct: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGG+LFLSLPAQLGRGGVLGITNVHLTAEES+RLRDSANTILQVQTELGI
Sbjct: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 350

[Close]

BLAST of Lsi09G004170.1 vs. NCBI nr
Match: gi|778700014|ref|XP_011654796.1| (PREDICTED: L-lactate dehydrogenase B-like [Cucumis sativus])

HSP 1 Score: 629.8 bits (1623), Expect = 2.9e-177
Identity = 327/350 (93.43%), Postives = 337/350 (96.29%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS STSSLGPGGLDLSQAFFKPIRDASP STS  N+KISVIG GNVGMAIAQTILTQD
Sbjct: 63  MQKSPSTSSLGPGGLDLSQAFFKPIRDASPLSTSNCNTKISVIGTGNVGMAIAQTILTQD 122

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LV+ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITA SDLCIVTAGARQIAG
Sbjct: 123 LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITARSDLCIVTAGARQIAG 182

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNV LFQKIVP LVQFSPETILLIVSNPVDVLTYVAWKLSGF SNRVIGSGT
Sbjct: 183 ESRLNLLQRNVTLFQKIVPLLVQFSPETILLIVSNPVDVLTYVAWKLSGFSSNRVIGSGT 242

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFLKNQQI YE
Sbjct: 243 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 302

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEV+GGAYEVI+LKGYTSWAIGYSVASLARS+LRDQRRIHPVSV+AKGFYGI
Sbjct: 303 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVIAKGFYGI 362

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           +GG+LFLSLPAQLGRGGVLGITNVHLT EES+RLRDSANTIL VQ ELGI
Sbjct: 363 EGGDLFLSLPAQLGRGGVLGITNVHLTTEESKRLRDSANTILHVQAELGI 412

[Close]

BLAST of Lsi09G004170.1 vs. NCBI nr
Match: gi|802581859|ref|XP_012069951.1| (PREDICTED: L-lactate dehydrogenase B [Jatropha curcas])

HSP 1 Score: 600.1 bits (1546), Expect = 2.4e-168
Identity = 301/350 (86.00%), Postives = 331/350 (94.57%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS S S+LGPGGLDL+QAFFKPI++ +PPS +KR++KISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASTLGPGGLDLTQAFFKPIQNTAPPSPTKRHTKISVIGTGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L +EL LVDAKPDKLRGEMLDLQHAAAFLPRTKI ASTDYS+T GSDLCIVTAGARQI G
Sbjct: 61  LADELALVDAKPDKLRGEMLDLQHAAAFLPRTKIIASTDYSVTVGSDLCIVTAGARQIPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF+KI+PPLV +SPE+IL+IVSNPVDVLTY+AWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFEKIIPPLVNYSPESILMIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFL+ QQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLEKQQIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           KETLE IHK V+  AYEVI+LKGYTSWAIGYS A+LARSLLRDQR+IHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKAVIDSAYEVINLKGYTSWAIGYSAANLARSLLRDQRKIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGGE+FLSLPAQLGRGGVLG+TNVHLT EE+RRLR+SA T+L+VQ++LGI
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNVHLTEEEARRLRESAKTLLEVQSQLGI 350

[Close]

BLAST of Lsi09G004170.1 vs. NCBI nr
Match: gi|823228917|ref|XP_012447203.1| (PREDICTED: L-lactate dehydrogenase B-like [Gossypium raimondii])

HSP 1 Score: 591.7 bits (1524), Expect = 8.7e-166
Identity = 298/350 (85.14%), Postives = 328/350 (93.71%), Query Frame = 1


Query: 1   MQKSSSTSSLGPGGLDLSQAFFKPIRDASPPSTSKRNSKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDL+QAFFKPI++  PPS++KR++KISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASSLGPGGLDLAQAFFKPIQNCDPPSSTKRHTKISVIGVGNVGMAIAQTILTQD 60

Query: 61  LVEELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L +EL LVDAK DKLRGEMLDLQHAAAFLPRTKI+AS DYS+TAGSDLCIVTAGARQ  G
Sbjct: 61  LADELALVDAKSDKLRGEMLDLQHAAAFLPRTKINASVDYSVTAGSDLCIVTAGARQNPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF  I+PPL ++SP++ILLIVSNPVDVLTYV+WKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFSNIIPPLAKYSPDSILLIVSNPVDVLTYVSWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSIS+GGVP+LSFLKNQQIAYE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISIGGVPVLSFLKNQQIAYE 240

Query: 241 KETLEKIHKEVVGGAYEVISLKGYTSWAIGYSVASLARSLLRDQRRIHPVSVLAKGFYGI 300
           K+TLE IHK VV  AYEVISLKGYTSWAIGYSVA+LARSLLRDQR+IHPVSVLAKGFYGI
Sbjct: 241 KQTLENIHKAVVESAYEVISLKGYTSWAIGYSVANLARSLLRDQRKIHPVSVLAKGFYGI 300

Query: 301 DGGELFLSLPAQLGRGGVLGITNVHLTAEESRRLRDSANTILQVQTELGI 351
           DGGE+FLSLPAQLGRGGVLG+TN+HLT EE +RLR SA TIL+VQ++LG+
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNIHLTDEEVQRLRKSAETILEVQSQLGL 350

The following BLAST results are available for this feature:

BLAST of Lsi09G004170.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
LDHA_HORVU	8.5e-129	69.83	L-lactate dehydrogenase A OS=Hordeum vulgare PE=1 SV=1	[more]
LDHB_HORVU	1.1e-125	67.84	L-lactate dehydrogenase B (Fragment) OS=Hordeum vulgare PE=1 SV=1	[more]
LDH_MAIZE	2.9e-121	66.48	L-lactate dehydrogenase OS=Zea mays PE=2 SV=1	[more]
LDHA_MOUSE	5.7e-93	55.63	L-lactate dehydrogenase A chain OS=Mus musculus GN=Ldha PE=1 SV=3	[more]
LDHA_RAT	4.8e-92	55.31	L-lactate dehydrogenase A chain OS=Rattus norvegicus GN=Ldha PE=1 SV=1	[more]

BLAST of Lsi09G004170.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KKU9_CUCSA	2.0e-177	93.43	L-lactate dehydrogenase OS=Cucumis sativus GN=Csa_5G161920 PE=3 SV=1	[more]
A0A067KUG8_JATCU	1.7e-168	86.00	L-lactate dehydrogenase OS=Jatropha curcas GN=JCGZ_03382 PE=3 SV=1	[more]
A0A0D2UW00_GOSRA	6.0e-166	85.14	L-lactate dehydrogenase OS=Gossypium raimondii GN=B456_009G297700 PE=3 SV=1	[more]
A0A151TJG6_CAJCA	7.9e-166	85.43	L-lactate dehydrogenase OS=Cajanus cajan GN=KK1_013505 PE=3 SV=1	[more]
A0A0B0P6A6_GOSAR	1.3e-165	85.14	L-lactate dehydrogenase OS=Gossypium arboreum GN=F383_25947 PE=3 SV=1	[more]

BLAST of Lsi09G004170.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G17260.1	1.1e-155	76.77	Lactate/malate dehydrogenase family protein	[more]

BLAST of Lsi09G004170.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659073513\|ref\|XP_008437100.1\|	2.2e-185	96.86	PREDICTED: L-lactate dehydrogenase B [Cucumis melo]	[more]
gi\|778700018\|ref\|XP_004151663.2\|	1.2e-183	95.71	PREDICTED: LOW QUALITY PROTEIN: L-lactate dehydrogenase A-like [Cucumis sativus]	[more]
gi\|778700014\|ref\|XP_011654796.1\|	2.9e-177	93.43	PREDICTED: L-lactate dehydrogenase B-like [Cucumis sativus]	[more]
gi\|802581859\|ref\|XP_012069951.1\|	2.4e-168	86.00	PREDICTED: L-lactate dehydrogenase B [Jatropha curcas]	[more]
gi\|823228917\|ref\|XP_012447203.1\|	8.7e-166	85.14	PREDICTED: L-lactate dehydrogenase B-like [Gossypium raimondii]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process
GO:0019752	carboxylic acid metabolic process
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0004459	L-lactate dehydrogenase activity
GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0016491	oxidoreductase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **INTERPRO**
Term	Definition
IPR022383	Lactate/malate_DH_C
IPR018177	L-lactate_DH_AS
IPR016040	NAD(P)-bd_dom
IPR015955	Lactate_DH/Glyco_Ohase_4_C
IPR011304	L-lactate_DH
IPR001557	L-lactate/malate_DH
IPR001236	Lactate/malate_DH_N

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0019752	carboxylic acid metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006534	cysteine metabolic process
biological_process	GO:0006094	gluconeogenesis
biological_process	GO:0006096	glycolytic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0004459	L-lactate dehydrogenase activity
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi09G004170	Lsi09G004170	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi09G004170.1.CDS.2	Lsi09G004170.1.CDS.2	CDS
Lsi09G004170.1.CDS.1	Lsi09G004170.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi09G004170.1	Lsi09G004170.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001236	Lactate/malate dehydrogenase, N-terminal	PFAM	PF00056	Ldh_1_N	coord: 39..177 score: 3.8
IPR001557	L-lactate/malate dehydrogenase	PRINTS	PR00086	LLDHDRGNASE	coord: 39..63 score: 8.9E-54coord: 64..88 score: 8.9E-54coord: 205..218 score: 8.9E-54coord: 175..193 score: 8.9E-54coord: 151..171 score: 8.9
IPR001557	L-lactate/malate dehydrogenase	PIR	PIRSF000102	Lac_mal_DH	coord: 34..350 score: 2.0E
IPR001557	L-lactate/malate dehydrogenase	PANTHER	PTHR11540	MALATE AND LACTATE DEHYDROGENASE	coord: 10..348 score: 6.7E
IPR011304	L-lactate dehydrogenase	HAMAP	MF_00488	Lactate_dehydrog	coord: 37..349 score: 37
IPR011304	L-lactate dehydrogenase	TIGRFAMs	TIGR01771	TIGR01771	coord: 42..341 score: 5.2E
IPR015955	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal	GENE3D	G3DSA:3.90.110.10		coord: 180..348 score: 6.6
IPR015955	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal	unknown	SSF56327	LDH C-terminal domain-like	coord: 178..346 score: 1.73
IPR016040	NAD(P)-binding domain	GENE3D	G3DSA:3.40.50.720		coord: 29..179 score: 1.1
IPR016040	NAD(P)-binding domain	unknown	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 24..177 score: 3.47
IPR018177	L-lactate dehydrogenase, active site	PROSITE	PS00064	L_LDH	coord: 207..213 scor
IPR022383	Lactate/malate dehydrogenase, C-terminal	PFAM	PF02866	Ldh_1_C	coord: 180..343 score: 1.9
None	No IPR available	PANTHER	PTHR11540:SF3	L-LACTATE DEHYDROGENASE	coord: 10..348 score: 6.7E