MELO3C025408 (gene) Melon (DHL92) v3.5.1

Overview
NameMELO3C025408
Typegene
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionThiamine thiazole synthase, chloroplastic
Locationchr6 : 29827895 .. 29829753 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CACACACCAAACTACAATCTCCTCTGTTTTCCTCTGCTTCAAAAATGGCTTCCATTGCTTCCACTCTCACCACCAAGCTCCAAATACCTTCTTTACTTCATGACTCCTCTTTCCATGGCACACCTCTCGCCTCACCTCCTTCTCTCCGCCTCAAATCCCCCGCCGCCACATCTCTCACCATCTCCGCCTCCGCCTCTCAGCCTCCCTACGATTTAAACCAATTCAAATTCAACCCTATCAGAGAATCTATCGTCTCTCGGGAGATGACCCGTCGTTACATGACCGATATGATTACCTACGCTGACACTGACGTTATCATCGTCGGCGCTGGCTCCGCTGGGCTCTCTTGCGCTTACGAACTCAGCAAAAACCCCAATATTCGTATTGCCATCATCGAACAATCCGTGAGCCCCGGTGGCGGTGCATGGCTCGGCGGCCAACTCTTCTCTGCTATGGTAAGAAATTCATTTCCTTTCCATATTTGAATGATGCTCTGTTTTTTTTTCTGTTTTGTTCTTTTCTTGAAAATAACAAAAATTTGAAAATCCCCCAATCAGCTTTCATGCATGCCGTATTTGGATTTAAAAAATCCTTTACTTTTTAACAGAGATCTGAAATAAAGAAACCCAGAAAGTAAAATCATTAATTTGAGAGGAAATAGTATACAGATCTTGTGATACCTTAAAAAATTGGATTAATTTGCGTCGGAAACAAATATTCTGATCTTGTTCAGCTGATTGATGTTGGTTAAATTTAATGTTTGATAGGTGGTCCGTAAGCCAGCCCATTTATTCTTGGATGAGGTGGGTGTGGAGTATGACGAGCAAGACGACTATGTCGTGATAAAGCACGCGGCTCTGTTCACGTCGACGATCATGAGCAAGCTCTTGGCCCGGCCGAACGTGAAGCTTTTCAACGCCGTGGCGGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGGGTCGTCACCAACTGGGCTCTTGTGTCAATGAACCATGACACCCAGTCCTGCATGGACCCCAATGTGATGGAGGCTAAGGTGGTTGTGAGCTCTTGTGGCCACGATGGACCTTTTGGCGCTACCGGAGTCAAGCGCCTCAAGAGCATCGGCTTGATCGATAGTGTCCCGGGAATGAAGGCCCTTGACATGAACACTGCTGAGGATGCCATTGTTCGATTCACTCGAGAGGTCGTTCCTGGCATGATTGTCACCGGCATGGAGGTTGCCGAGATCGATGGAGCTCCAAGAATGGTAAACGAACTAGAGAGATATTACTGCTTTAGCCCGTTTAATAATCATGATACAGTAAAATATAACAGCGTTTAGATGAGTTTACACGCTTCACACTAATAACTTATTTTACTCCGGCGGCTATCTGCATTAGATTACCACCGAAAGATTTTGTTATACCCAAATTTATTAGTCTTTAAACCGTGCCATTCATAGGGGCCAACGTTCGGGGCGATGATGATATCAGGGCAGAAGGCGGCACACTTGGCACTAAAGTCATTGGGAGAGGCGAACAGCATTGGAGGTGAAGGAGAGATGGGAGAAAGAAAGGTTGGACTTGAAGAGGAAGAGTTGCTACTGGCAGCGGGAGAGTCGCCGGAGGTTGCAGACGCTTAAAGTTTTTAATGGCGGGCGGATGTTGATTTGGAAGAAATATTTGCTTTTGGGTTTTATGGTGCTGTTTGTTTGTTTGTAGTGAGTTTTCAGTAAACGAAAAGGGGGGTTGGTTGTGTTAGGCTTTTTTGTGTTTTCATAACACAATGGGGTTTGGGGAAAGACAAAAAATATCAGTTGCCTTTTTCTCTCTCTCTTTCTTTCTCTTTTTATTTTGTTAATAAAAATGCC

mRNA sequence

CACACACCAAACTACAATCTCCTCTGTTTTCCTCTGCTTCAAAAATGGCTTCCATTGCTTCCACTCTCACCACCAAGCTCCAAATACCTTCTTTACTTCATGACTCCTCTTTCCATGGCACACCTCTCGCCTCACCTCCTTCTCTCCGCCTCAAATCCCCCGCCGCCACATCTCTCACCATCTCCGCCTCCGCCTCTCAGCCTCCCTACGATTTAAACCAATTCAAATTCAACCCTATCAGAGAATCTATCGTCTCTCGGGAGATGACCCGTCGTTACATGACCGATATGATTACCTACGCTGACACTGACGTTATCATCGTCGGCGCTGGCTCCGCTGGGCTCTCTTGCGCTTACGAACTCAGCAAAAACCCCAATATTCGTATTGCCATCATCGAACAATCCGTGAGCCCCGGTGGCGGTGCATGGCTCGGCGGCCAACTCTTCTCTGCTATGGTGGTCCGTAAGCCAGCCCATTTATTCTTGGATGAGGTGGGTGTGGAGTATGACGAGCAAGACGACTATGTCGTGATAAAGCACGCGGCTCTGTTCACGTCGACGATCATGAGCAAGCTCTTGGCCCGGCCGAACGTGAAGCTTTTCAACGCCGTGGCGGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGGGTCGTCACCAACTGGGCTCTTGTGTCAATGAACCATGACACCCAGTCCTGCATGGACCCCAATGTGATGGAGGCTAAGGTGGTTGTGAGCTCTTGTGGCCACGATGGACCTTTTGGCGCTACCGGAGTCAAGCGCCTCAAGAGCATCGGCTTGATCGATAGTGTCCCGGGAATGAAGGCCCTTGACATGAACACTGCTGAGGATGCCATTGTTCGATTCACTCGAGAGGTCGTTCCTGGCATGATTGTCACCGGCATGGAGGTTGCCGAGATCGATGGAGCTCCAAGAATGGGGCCAACGTTCGGGGCGATGATGATATCAGGGCAGAAGGCGGCACACTTGGCACTAAAGTCATTGGGAGAGGCGAACAGCATTGGAGGTGAAGGAGAGATGGGAGAAAGAAAGGTTGGACTTGAAGAGGAAGAGTTGCTACTGGCAGCGGGAGAGTCGCCGGAGGTTGCAGACGCTTAAAGTTTTTAATGGCGGGCGGATGTTGATTTGGAAGAAATATTTGCTTTTGGGTTTTATGGTGCTGTTTGTTTGTTTGTAGTGAGTTTTCAGTAAACGAAAAGGGGGGTTGGTTGTGTTAGGCTTTTTTGTGTTTTCATAACACAATGGGGTTTGGGGAAAGACAAAAAATATCAGTTGCCTTTTTCTCTCTCTCTTTCTTTCTCTTTTTATTTTGTTAATAAAAATGCC

Coding sequence (CDS)

ATGGCTTCCATTGCTTCCACTCTCACCACCAAGCTCCAAATACCTTCTTTACTTCATGACTCCTCTTTCCATGGCACACCTCTCGCCTCACCTCCTTCTCTCCGCCTCAAATCCCCCGCCGCCACATCTCTCACCATCTCCGCCTCCGCCTCTCAGCCTCCCTACGATTTAAACCAATTCAAATTCAACCCTATCAGAGAATCTATCGTCTCTCGGGAGATGACCCGTCGTTACATGACCGATATGATTACCTACGCTGACACTGACGTTATCATCGTCGGCGCTGGCTCCGCTGGGCTCTCTTGCGCTTACGAACTCAGCAAAAACCCCAATATTCGTATTGCCATCATCGAACAATCCGTGAGCCCCGGTGGCGGTGCATGGCTCGGCGGCCAACTCTTCTCTGCTATGGTGGTCCGTAAGCCAGCCCATTTATTCTTGGATGAGGTGGGTGTGGAGTATGACGAGCAAGACGACTATGTCGTGATAAAGCACGCGGCTCTGTTCACGTCGACGATCATGAGCAAGCTCTTGGCCCGGCCGAACGTGAAGCTTTTCAACGCCGTGGCGGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGGGTCGTCACCAACTGGGCTCTTGTGTCAATGAACCATGACACCCAGTCCTGCATGGACCCCAATGTGATGGAGGCTAAGGTGGTTGTGAGCTCTTGTGGCCACGATGGACCTTTTGGCGCTACCGGAGTCAAGCGCCTCAAGAGCATCGGCTTGATCGATAGTGTCCCGGGAATGAAGGCCCTTGACATGAACACTGCTGAGGATGCCATTGTTCGATTCACTCGAGAGGTCGTTCCTGGCATGATTGTCACCGGCATGGAGGTTGCCGAGATCGATGGAGCTCCAAGAATGGGGCCAACGTTCGGGGCGATGATGATATCAGGGCAGAAGGCGGCACACTTGGCACTAAAGTCATTGGGAGAGGCGAACAGCATTGGAGGTGAAGGAGAGATGGGAGAAAGAAAGGTTGGACTTGAAGAGGAAGAGTTGCTACTGGCAGCGGGAGAGTCGCCGGAGGTTGCAGACGCTTAA

Protein sequence

MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA*
Homology
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BLAST of MELO3C025408 vs. Swiss-Prot
Match: THI4_CITSI (Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis GN=THI1 PE=2 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 9.9e-157
Identity = 291/364 (79.95%), Postives = 318/364 (87.36%), Query Frame = 1 

Query: 1   MASIASTL--TTKLQIPSLLHDSSFHGTPLASPPSLRL---KSPAATSLTISASASQPPY 60
           MA++AST    +     + L DSSFHG P+ SP  LRL   KS    +L+I ++++ PPY
Sbjct: 1   MAAMASTAFAPSVSSTTNKLFDSSFHGAPM-SPSLLRLQPIKSSRPNNLSI-SASASPPY 60

Query: 61  DLNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIA 120
           DLN FKF+PI+ESIVSREMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSKNPNI+IA
Sbjct: 61  DLNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQIA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAH+FLDE+G++YDEQD+YVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIG+I+ VPGMKALDMN+AEDAIVR TREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPE 360
           GAPRMGPTFGAMMISGQKAAHLALKSLG+ N++ G        VG    EL+LAA +S E
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDG------TYVGGVHPELILAAADSAE 356

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BLAST of MELO3C025408 vs. Swiss-Prot
Match: THI4_ALNGL (Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa GN=THI1 PE=2 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 6.2e-151
Identity = 282/361 (78.12%), Postives = 314/361 (86.98%), Query Frame = 1 

Query: 4   IASTLTTKLQIPSLLHDS--SFHGTPLASPPSLRL---KSPAATSLTISASASQPPYDLN 63
           +ASTLT+K Q  +L  +S  SF+GTPLA P S+R+   K+ A  S+++S + S PPYDL 
Sbjct: 1   MASTLTSKPQRLALFENSASSFYGTPLA-PSSIRVQPTKAGAKPSISMSGAPS-PPYDLK 60

Query: 64  QFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIE 123
            F F+PI+ESIVSREMTRRYM DMITYADTDV++VGAGS+GL C YELSKNP++++AIIE
Sbjct: 61  AFTFDPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSSGLVC-YELSKNPSVQVAIIE 120

Query: 124 QSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLL 183
           QSVSPGGGAWLGGQLFS MVVRKPAHLFLDE+G+EYDEQD+YVVIKHAALFTSTIMSKLL
Sbjct: 121 QSVSPGGGAWLGGQLFSGMVVRKPAHLFLDELGIEYDEQDNYVVIKHAALFTSTIMSKLL 180

Query: 184 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 243
           ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD
Sbjct: 181 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240

Query: 244 GPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAP 303
           GPFGATGVK L+SIG+ID+VPGMKALDMN AEDAIVR TRE+VPGMIVTGMEVAEIDGAP
Sbjct: 241 GPFGATGVKSLRSIGMIDTVPGMKALDMNVAEDAIVRLTREIVPGMIVTGMEVAEIDGAP 300

Query: 304 RMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVAD 360
           RMGPTFGAMMISGQKAAHLALK+LG  N++ G        VG    EL+LAA +S E+AD
Sbjct: 301 RMGPTFGAMMISGQKAAHLALKALGLPNALDGS------YVGGIHPELILAAADSAEIAD 352

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BLAST of MELO3C025408 vs. Swiss-Prot
Match: THI42_VITVI (Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera GN=THI1-2 PE=3 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 1.4e-150
Identity = 284/365 (77.81%), Postives = 309/365 (84.66%), Query Frame = 1 

Query: 1   MASIASTLTTKLQIPSLL---HDSSFHGTPLAS---PPSLRLKSPAATSLTISASASQPP 60
           MAS+A+TLT+    P      + SSFHG+P++    P  +  +SP     TIS S S  P
Sbjct: 1   MASMATTLTSLSSNPKPAFFDNKSSFHGSPISYRVLPIKVSHQSP-----TISMS-SVSP 60

Query: 61  YDLNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRI 120
           YDL  FKF PI+ESIV+REMTRRYM DMITYADTDV+IVGAGSAGLSCAYELSKNP+IR+
Sbjct: 61  YDLQSFKFEPIKESIVAREMTRRYMMDMITYADTDVVIVGAGSAGLSCAYELSKNPSIRV 120

Query: 121 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIM 180
           AIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQD+YVVIKHAALFTSTIM
Sbjct: 121 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHHFLDELGIEYDEQDNYVVIKHAALFTSTIM 180

Query: 181 SKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 240
           SKLLARPNVKLFNAVAAEDLIVK  RV GVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS
Sbjct: 181 SKLLARPNVKLFNAVAAEDLIVKEERVAGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 240

Query: 241 CGHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEI 300
           CGHDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TRE+VPGMIVTGMEVAEI
Sbjct: 241 CGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEI 300

Query: 301 DGAPRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESP 360
           DGAPRMGPTFGAMMISGQKAAHLAL++LG+ N+I G     E      + EL+LAA E+ 
Sbjct: 301 DGAPRMGPTFGAMMISGQKAAHLALRALGQPNAIDGNYTEAETM----QPELILAAAETG 355

[Close]
BLAST of MELO3C025408 vs. Swiss-Prot
Match: THI41_VITVI (Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera GN=THI1-1 PE=3 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 1.5e-149
Identity = 272/360 (75.56%), Postives = 309/360 (85.83%), Query Frame = 1 

Query: 1   MASIASTLTTKLQIPSL-LHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQ 60
           MA++ S++ +K +      H SSFHG P+A+    RL    +T + ++ +A+  PYDL  
Sbjct: 1   MATLTSSICSKPKASVFDPHKSSFHGVPIAT--QARLSPVKSTPVNLAVTAAAMPYDLRS 60

Query: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           FKF PI+ESIVSREMTRRYM DMITYADTDV++VGAGSAGLSCAYELSKNP++++AIIEQ
Sbjct: 61  FKFEPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPSVQVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFS+MVVRKPAH FLDE+G+EYDEQD+YVVIKHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSSMVVRKPAHRFLDELGLEYDEQDNYVVIKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLI+K G+VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIIKEGKVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRL+S+G+IDSVPGMKALDMNTAED IVR TREVVPGMIVTGMEVAEIDG+PR
Sbjct: 241 PFGATGVKRLRSVGMIDSVPGMKALDMNTAEDEIVRLTREVVPGMIVTGMEVAEIDGSPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 360
           MGPTFGAMMISGQKAAHLALKSLG  N++ G   +G     L  E +L AA ++ E+A+A
Sbjct: 301 MGPTFGAMMISGQKAAHLALKSLGLPNALDGT-YIG----NLHPELVLAAAADAAEIAEA 353

[Close]
BLAST of MELO3C025408 vs. Swiss-Prot
Match: THI4_ARATH (Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana GN=THI1 PE=1 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 2.6e-149
Identity = 277/360 (76.94%), Postives = 302/360 (83.89%), Query Frame = 1 

Query: 1   MASIASTLTTKLQIPSLLHDSSFHGTPL-ASPPSLRLKSPAATSLTISASASQPPYDLNQ 60
           MA+IASTL+     P  L DSSFHG+ + A+P S+ LK  +      S  A+   YDLN 
Sbjct: 1   MAAIASTLSLSSTKPQRLFDSSFHGSAISAAPISIGLKPRS-----FSVRATTAGYDLNA 60

Query: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           F F+PI+ESIVSREMTRRYMTDMITYA+TDV++VGAGSAGLS AYE+SKNPN+++AIIEQ
Sbjct: 61  FTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAM+VRKPAHLFLDE+GV YDEQD YVV+KHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIG+ID VPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 360
           MGPTFGAMMISGQKA  LALK+LG  N+I G        VG    EL+LAA +S E  DA
Sbjct: 301 MGPTFGAMMISGQKAGQLALKALGLPNAIDG------TLVGNLSPELVLAAADSAETVDA 349

[Close]
BLAST of MELO3C025408 vs. TrEMBL
Match: A0A0A0L546_CUCSA (Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus GN=THI1 PE=3 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 3.7e-195
Identity = 353/359 (98.33%), Postives = 355/359 (98.89%), Query Frame = 1 

Query: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF 60
           MASIASTLTTKLQ PSLLHDSSFHGTPLASP SLRLKS AATSLTISASASQPPYDLNQF
Sbjct: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDLNQF 60

Query: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120
           KFNPIRESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNPNIRIAIIEQS
Sbjct: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120

Query: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180
           VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR
Sbjct: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180

Query: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240

Query: 241 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300
           FGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 241 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300

Query: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 360
           GPTFGAMMISGQKAAHLALKSLGEANSIG EGEMGERKVGLEEEELLLAAGESPEVADA
Sbjct: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 359

[Close]
BLAST of MELO3C025408 vs. TrEMBL
Match: A0A061F760_THECC (Thiamine thiazole synthase, chloroplastic OS=Theobroma cacao GN=THI1 PE=3 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 3.4e-156
Identity = 289/363 (79.61%), Postives = 322/363 (88.71%), Query Frame = 1 

Query: 2   ASIASTLT--TKLQIPSLLHDSSFHGTPLASPPSLRLK---SPAATSLTISASASQPPYD 61
           +S+A+TLT  TKL   + L +SSFHG P+ +P S  LK   SP   S+++SA++S PPYD
Sbjct: 3   SSVATTLTSSTKLYRNTSLFESSFHGVPI-TPLSFHLKAKSSPRNASVSMSAASSPPPYD 62

Query: 62  LNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAI 121
           LN F+F+PI+ESIVSREMTRRYM DMIT+ADTDV++VGAGSAGLSCAYELSKNP++++AI
Sbjct: 63  LNNFRFDPIKESIVSREMTRRYMMDMITHADTDVVVVGAGSAGLSCAYELSKNPSVQVAI 122

Query: 122 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 181
           +EQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQDDYVVIKHAALFTSTIMSK
Sbjct: 123 VEQSVSPGGGAWLGGQLFSAMVVRKPAHRFLDELGIEYDEQDDYVVIKHAALFTSTIMSK 182

Query: 182 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 241
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 183 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 242

Query: 242 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 301
           HDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TRE+VPGMIVTGMEVAEIDG
Sbjct: 243 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEIDG 302

Query: 302 APRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEV 360
           +PRMGPTFGAMMISGQKAAHLAL+SLG  N++ G        VG    EL+LAA +S E 
Sbjct: 303 SPRMGPTFGAMMISGQKAAHLALQSLGLPNALDG------TYVGNIHPELILAAADSAET 358

[Close]
BLAST of MELO3C025408 vs. TrEMBL
Match: A0A067ECZ8_CITSI (Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis GN=THI1 PE=3 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 3.2e-154
Identity = 290/363 (79.89%), Postives = 319/363 (87.88%), Query Frame = 1 

Query: 1   MASIASTL--TTKLQIPSLLHDSSFHGTPLASPPSLRL---KSPAATSLTISASASQPPY 60
           MA++AST    +     + L DSSFHG P+ SP  LRL   KS    +L+I ++++ PPY
Sbjct: 1   MAAMASTAFAPSVSSTTNKLFDSSFHGAPM-SPSLLRLQPIKSSRPNNLSI-SASASPPY 60

Query: 61  DLNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIA 120
           DLN FKF+PI+ESIVSREMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSKNPNI+IA
Sbjct: 61  DLNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQIA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAH+FLDE+G++YDEQD+YVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIG+I+ VPGMKALDMN+AEDAIVR TREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPE 359
           GAPRMGPTFGAMMISGQKAAHLALKSLG+ N++ G        VG    EL+LAA +S E
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDG------TYVGGVHPELILAAADSAE 355

[Close]
BLAST of MELO3C025408 vs. TrEMBL
Match: V4TXU9_9ROSI (Thiamine thiazole synthase, chloroplastic OS=Citrus clementina GN=THI1 PE=3 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 9.3e-154
Identity = 285/345 (82.61%), Postives = 310/345 (89.86%), Query Frame = 1 

Query: 18  LHDSSFHGTPLASPPSLRL---KSPAATSLTISASASQPPYDLNQFKFNPIRESIVSREM 77
           L DSSFHG P+ SP  LRL   KS    +L+I ++++ PPYDLN FKF+PI+ESIVSREM
Sbjct: 20  LFDSSFHGAPM-SPSLLRLQPIKSSRPNNLSI-SASASPPYDLNTFKFDPIKESIVSREM 79

Query: 78  TRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQSVSPGGGAWLGGQLF 137
           TRRYMTDMITYADTDV++VGAGSAGLSCAYELSKNPNI+IAIIEQSVSPGGGAWLGGQLF
Sbjct: 80  TRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQIAIIEQSVSPGGGAWLGGQLF 139

Query: 138 SAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLARPNVKLFNAVAAEDL 197
           SAMVVRKPAH+FLDE+G++YDEQD+YVVIKHAALFTSTIMSKLLARPNVKLFNAVAAEDL
Sbjct: 140 SAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIMSKLLARPNVKLFNAVAAEDL 199

Query: 198 IVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGL 257
           IVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIG+
Sbjct: 200 IVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGM 259

Query: 258 IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKA 317
           I+ VPGMKALDMN+AEDAIVR TREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKA
Sbjct: 260 IEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKA 319

Query: 318 AHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 360
           AHLAL SLG+ N++ G        VG    EL+LAA +S E ADA
Sbjct: 320 AHLALNSLGQPNALDG------TYVGGVHPELILAAADSAETADA 356

[Close]
BLAST of MELO3C025408 vs. TrEMBL
Match: M5WMT5_PRUPE (Thiamine thiazole synthase, chloroplastic OS=Prunus persica GN=THI1 PE=3 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 1.6e-153
Identity = 287/367 (78.20%), Postives = 321/367 (87.47%), Query Frame = 1 

Query: 1   MASIASTL-----TTKLQIPSLLHDSSFHGTPLASPPSLRLKSPA---ATSLTISASASQ 60
           MAS+ASTL     TTKLQ PSLL DSSFHGT +      +  + +     S+++SA+ S 
Sbjct: 1   MASMASTLISSPLTTKLQKPSLL-DSSFHGTSVVRLQPAKAATNSNNHGLSVSMSANGST 60

Query: 61  PPYDLNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNI 120
           P YDL+ FKF+PI+ESIVSREMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSKNPN+
Sbjct: 61  PSYDLSAFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNV 120

Query: 121 RIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTST 180
           ++AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFL+E+G+ YDE+D YVVIKHAALFTST
Sbjct: 121 QVAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLNELGIAYDEKDHYVVIKHAALFTST 180

Query: 181 IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV 240
           IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV
Sbjct: 181 IMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVV 240

Query: 241 SSCGHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVA 300
           SSCGHDGP GATGVKRL+SIG+I+SVPGMKALDMNTAEDAIV+ TRE+VPGMIVTGMEVA
Sbjct: 241 SSCGHDGPMGATGVKRLRSIGMIESVPGMKALDMNTAEDAIVKLTREIVPGMIVTGMEVA 300

Query: 301 EIDGAPRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGE 360
           EIDG+PRMGPTFGAMMISGQKAAHLALKSLG  N++ G        VG  + EL+LAA +
Sbjct: 301 EIDGSPRMGPTFGAMMISGQKAAHLALKSLGLPNALDGS------YVGSIQPELILAAAD 360

[Close]
BLAST of MELO3C025408 vs. TAIR10
Match: AT5G54770.1 (AT5G54770.1 thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4))

HSP 1 Score: 529.6 bits (1363), Expect = 1.5e-150
Identity = 277/360 (76.94%), Postives = 302/360 (83.89%), Query Frame = 1 

Query: 1   MASIASTLTTKLQIPSLLHDSSFHGTPL-ASPPSLRLKSPAATSLTISASASQPPYDLNQ 60
           MA+IASTL+     P  L DSSFHG+ + A+P S+ LK  +      S  A+   YDLN 
Sbjct: 1   MAAIASTLSLSSTKPQRLFDSSFHGSAISAAPISIGLKPRS-----FSVRATTAGYDLNA 60

Query: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           F F+PI+ESIVSREMTRRYMTDMITYA+TDV++VGAGSAGLS AYE+SKNPN+++AIIEQ
Sbjct: 61  FTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAM+VRKPAHLFLDE+GV YDEQD YVV+KHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIG+ID VPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 360
           MGPTFGAMMISGQKA  LALK+LG  N+I G        VG    EL+LAA +S E  DA
Sbjct: 301 MGPTFGAMMISGQKAGQLALKALGLPNAIDG------TLVGNLSPELVLAAADSAETVDA 349

[Close]
BLAST of MELO3C025408 vs. NCBI nr
Match: gi|659126941|ref|XP_008463440.1| (PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo])

HSP 1 Score: 701.4 bits (1809), Expect = 8.0e-199
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = 1 

Query: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF 60
           MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF
Sbjct: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF 60

Query: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120
           KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS
Sbjct: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120

Query: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180
           VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR
Sbjct: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180

Query: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240

Query: 241 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300
           FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 241 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300

Query: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 360
           GPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA
Sbjct: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 359

[Close]
BLAST of MELO3C025408 vs. NCBI nr
Match: gi|449470423|ref|XP_004152916.1| (PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis sativus])

HSP 1 Score: 688.7 bits (1776), Expect = 5.3e-195
Identity = 353/359 (98.33%), Postives = 355/359 (98.89%), Query Frame = 1 

Query: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF 60
           MASIASTLTTKLQ PSLLHDSSFHGTPLASP SLRLKS AATSLTISASASQPPYDLNQF
Sbjct: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDLNQF 60

Query: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120
           KFNPIRESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNPNIRIAIIEQS
Sbjct: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120

Query: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180
           VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR
Sbjct: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180

Query: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240

Query: 241 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300
           FGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 241 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300

Query: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 360
           GPTFGAMMISGQKAAHLALKSLGEANSIG EGEMGERKVGLEEEELLLAAGESPEVADA
Sbjct: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 359

[Close]
BLAST of MELO3C025408 vs. NCBI nr
Match: gi|590607577|ref|XP_007021022.1| (Thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) [Theobroma cacao])

HSP 1 Score: 559.3 bits (1440), Expect = 4.9e-156
Identity = 289/363 (79.61%), Postives = 322/363 (88.71%), Query Frame = 1 

Query: 2   ASIASTLT--TKLQIPSLLHDSSFHGTPLASPPSLRLK---SPAATSLTISASASQPPYD 61
           +S+A+TLT  TKL   + L +SSFHG P+ +P S  LK   SP   S+++SA++S PPYD
Sbjct: 3   SSVATTLTSSTKLYRNTSLFESSFHGVPI-TPLSFHLKAKSSPRNASVSMSAASSPPPYD 62

Query: 62  LNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAI 121
           LN F+F+PI+ESIVSREMTRRYM DMIT+ADTDV++VGAGSAGLSCAYELSKNP++++AI
Sbjct: 63  LNNFRFDPIKESIVSREMTRRYMMDMITHADTDVVVVGAGSAGLSCAYELSKNPSVQVAI 122

Query: 122 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 181
           +EQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQDDYVVIKHAALFTSTIMSK
Sbjct: 123 VEQSVSPGGGAWLGGQLFSAMVVRKPAHRFLDELGIEYDEQDDYVVIKHAALFTSTIMSK 182

Query: 182 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 241
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 183 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 242

Query: 242 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 301
           HDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TRE+VPGMIVTGMEVAEIDG
Sbjct: 243 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEIDG 302

Query: 302 APRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEV 360
           +PRMGPTFGAMMISGQKAAHLAL+SLG  N++ G        VG    EL+LAA +S E 
Sbjct: 303 SPRMGPTFGAMMISGQKAAHLALQSLGLPNALDG------TYVGNIHPELILAAADSAET 358

[Close]
BLAST of MELO3C025408 vs. NCBI nr
Match: gi|729356390|ref|XP_010545204.1| (PREDICTED: thiamine thiazole synthase, chloroplastic [Tarenaya hassleriana])

HSP 1 Score: 556.2 bits (1432), Expect = 4.1e-155
Identity = 287/364 (78.85%), Postives = 318/364 (87.36%), Query Frame = 1 

Query: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPP-SLRLKSPAAT----SLTISASASQPPY 60
           MA++AST++     P  L DSSFHG+ +A PP S+ L+S A      S+  SA +S PPY
Sbjct: 1   MAAMASTISLSSTKPQRLFDSSFHGSAVAVPPVSVGLRSRAVAGNGFSVRASAESSSPPY 60

Query: 61  DLNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIA 120
           DLN F+F+PI+ESIVSREMTRRYMTDMITYA+TDV+IVGAGSAGLSCAYE+SKNPN+++A
Sbjct: 61  DLNNFRFSPIKESIVSREMTRRYMTDMITYAETDVVIVGAGSAGLSCAYEISKNPNVQVA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAH+FLDE+ +EYDEQDDYVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHMFLDELDIEYDEQDDYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKG RVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIG+ID+VPGMKALDMN+AEDAIVR TREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDNVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPE 360
           GA RMGPTFGAMMISGQKAAHLALK+LG  N++     +G+        EL+LAA ES E
Sbjct: 301 GAARMGPTFGAMMISGQKAAHLALKALGMPNALDDGSYLGKL-----HPELVLAAAESAE 359

[Close]
BLAST of MELO3C025408 vs. NCBI nr
Match: gi|572152967|ref|NP_001275783.1| (thiamine thiazole synthase, chloroplastic [Citrus sinensis])

HSP 1 Score: 554.3 bits (1427), Expect = 1.6e-154
Identity = 291/364 (79.95%), Postives = 320/364 (87.91%), Query Frame = 1 

Query: 1   MASIASTL--TTKLQIPSLLHDSSFHGTPLASPPSLRL---KSPAATSLTISASASQPPY 60
           MA++AST    +     + L DSSFHG P+ SP  LRL   KS    +L+I ++++ PPY
Sbjct: 1   MAAMASTAFAPSVSSTTNKLFDSSFHGAPM-SPSLLRLQPIKSSRPNNLSI-SASASPPY 60

Query: 61  DLNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIA 120
           DLN FKF+PI+ESIVSREMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSKNPNI+IA
Sbjct: 61  DLNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQIA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAH+FLDE+G++YDEQD+YVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIG+I+ VPGMKALDMN+AEDAIVR TREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPE 360
           GAPRMGPTFGAMMISGQKAAHLALKSLG+ N++ G        VG    EL+LAA +S E
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDG------TYVGGVHPELILAAADSAE 356

The following BLAST results are available for this feature:
BLAST of MELO3C025408 vs. Swiss-Prot
Match NameE-valueIdentityDescription
THI4_CITSI9.9e-15779.95Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis GN=THI1 PE=2 SV=1[more]
THI4_ALNGL6.2e-15178.12Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa GN=THI1 PE=2 SV=1[more]
THI42_VITVI1.4e-15077.81Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera GN=THI1-2 PE=3 SV=... [more]
THI41_VITVI1.5e-14975.56Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera GN=THI1-1 PE=3 SV=... [more]
THI4_ARATH2.6e-14976.94Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana GN=THI1 PE=1 S... [more]
BLAST of MELO3C025408 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L546_CUCSA3.7e-19598.33Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus GN=THI1 PE=3 SV=1[more]
A0A061F760_THECC3.4e-15679.61Thiamine thiazole synthase, chloroplastic OS=Theobroma cacao GN=THI1 PE=3 SV=1[more]
A0A067ECZ8_CITSI3.2e-15479.89Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis GN=THI1 PE=3 SV=1[more]
V4TXU9_9ROSI9.3e-15482.61Thiamine thiazole synthase, chloroplastic OS=Citrus clementina GN=THI1 PE=3 SV=1[more]
M5WMT5_PRUPE1.6e-15378.20Thiamine thiazole synthase, chloroplastic OS=Prunus persica GN=THI1 PE=3 SV=1[more]
BLAST of MELO3C025408 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G54770.11.5e-15076.94 thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)[more]
BLAST of MELO3C025408 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659126941|ref|XP_008463440.1|8.0e-199100.00PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo][more]
gi|449470423|ref|XP_004152916.1|5.3e-19598.33PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis sativus][more]
gi|590607577|ref|XP_007021022.1|4.9e-15679.61Thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) [Theobroma cacao][more]
gi|729356390|ref|XP_010545204.1|4.1e-15578.85PREDICTED: thiamine thiazole synthase, chloroplastic [Tarenaya hassleriana][more]
gi|572152967|ref|NP_001275783.1|1.6e-15479.95thiamine thiazole synthase, chloroplastic [Citrus sinensis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002922Thi4_fam
IPR023753FAD/NAD-binding_dom
IPR027495Sti35
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO:0006950response to stress
GO:0009228thiamine biosynthetic process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006974 cellular response to DNA damage stimulus
biological_process GO:0009228 thiamine biosynthetic process
biological_process GO:0052837 thiazole biosynthetic process
biological_process GO:0018131 oxazole or thiazole biosynthetic process
biological_process GO:0006950 response to stress
cellular_component GO:0009579 thylakoid
cellular_component GO:0010319 stromule
cellular_component GO:0005739 mitochondrion
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0005829 cytosol
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0009507 chloroplast
cellular_component GO:0005575 cellular_component
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0042803 protein homodimerization activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003674 molecular_function
Unigene
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
MU45104melon EST collection version 4.0transcribed_cluster
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C025408T1MELO3C025408T1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
MU45104MU45104transcribed_cluster


InterPro
Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002922Thiazole biosynthetic enzyme Thi4 familyTIGRFAMsTIGR00292TIGR00292coord: 67..322
score: 1.8
IPR023753FAD/NAD(P)-binding domainGENE3DG3DSA:3.50.50.60coord: 87..321
score: 1.0E
IPR023753FAD/NAD(P)-binding domainunknownSSF51905FAD/NAD(P)-binding domaincoord: 47..316
score: 7.47
IPR027495Thiamine thiazole synthaseHAMAPMF_03158THI4coord: 5..331
score: 68
NoneNo IPR availablePRINTSPR00411PNDRDTASEIcoord: 344..358
score: 7.8E-5coord: 89..111
score: 7.
NoneNo IPR availablePANTHERPTHR10617ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASEcoord: 63..334
score: 2.6E
NoneNo IPR availablePANTHERPTHR10617:SF115THIAMINE THIAZOLE SYNTHASEcoord: 63..334
score: 2.6E
NoneNo IPR availablePFAMPF01946Thi4coord: 71..304
score: 1.1E