Carg00054 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg00054
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Thiamine thiazole synthase, chloroplastic
Location	Cucurbita_argyrosperma_scaffold_001 : 232134 .. 233782 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGCCCTTCCCCATCCCATCTCCTCTGTTTTCCTCTGCTTCTACAAAAATGGCTTCCATGGCTTCCACTCTCACCACCAAGCTCCAAACCCCCTCCTCCTCCTCCTCCTCCTTACTTGACTCCTCTTTCCATGGCACCCCTCTGGCCCCTCCTCCTTCTCTCCGCCTCAAACCCACCCCCGCCTCTTTCTCCATCTCTGCCTCCGCCTCTCAGCAGCAGCCTTACGATTTGAACTCCTTCAAATTCAACCCCATCAAGGAGTCCATCGTCTCACGTGAGATGACCCGCCGTTACATGACCGACATGATCACTTACGCCGATACTGATGTCGTCATCGTCGGCGCTGGCTCTGCTGGCCTCTCTTGCGCTTATGAACTTAGCAAAAACCCTGATATTCGCATCGCTATCATTGAGCAGTCCGTCTCCCCCGGCGGCGGTGCATGGCTCGGTGGCCAGCTCTTCTCTGCTATGGTATGCTTTTGATTTCCTTATATGCCATTTATTTTCCTCACCCTTACCCATAACGCCTCCTTTTCTTTCCTGTCTTTTAGTTTTGAATGCTCTGTTTTTTGTTCTTTGTAAGAAAATACCCACAATTAGATCATGCTAATTCAGTCATAAATTGGCTTAAGCCCAAGAATTTAAATACTCAATCAGTACAAGATCTAATTCTAACATCTTTAATCTGATGGGTTTGGGCAAATTTAATGTTTGGTTCATGTTCTTTGATAGGTGGTAAGGAAGCCAGCCCACTGCTTCTTGGACGAGGTTGGTGTGGAGTACGACGAGCAAGAGGACTACGTTGTAATTAAGCACGCGGCTCTGTTCACCTCAACGATCATGAGCAAGCTGCTGGCACGGCCCAATGTCAAGCTGTTCAACGCCGTGGCGGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGTGTCGTCACCAACTGGGCTTTAGTGTCAATGAACCATGACACACAGTCCTGCATGGACCCTAATGTCATGGAGGCGAAGGTAGTGGTCAGCTCTTGTGGCCACGACGGGCCCTTCGGAGCCACCGGAGTGAAGCGACTTAAGAGCATTGGCATGATCGATTCCGTCCCGGGAATGAAGGCCCTGGACATGAACACGGCGGAGGATGCCATTGTTAGGCTGACCCGAGAGGTGGTGCCTGGCATGATCGTAACGGGTATGGAGGTGGCGGAGATCGACGGAGCTCCAAGAATGGTAAAGAACTAAGTTTAAATTAATGGTTTAACTAAAAAGCTCAGACCTTTTTTAACAATCAGAGGTGGTGCTGGATGCAGGGGCCAACGTTCGGGGCAATGATGATATCGGGGCAGAAGGCGGCGCACCTGGCGTTGAAGTCATTGGGGCTGGCAAATTGCATTGAGGATGAAGCAGCTGATCTGATACTGGCGGCGGGAGAGTCACCGGAGGTTGCAGATGGTTGAATGGGTGAGTTTTCATGGATGGAAGGCGGCGGTTGTGTTTTGTGATTCATGGGGGCTCTGTTTCCGTGGCAAACAGATTGGGGTTTGGTGAAAAGCCAATTTTAAGTTAAATTAAATAAAATTGTATCAAAATGTTAATTGCCTTTTTCTTGTTAAATTATTATAAAATTAGATTTAAATTAATAATTTTGTAGTTAATAAA

mRNA sequence

CGCCCTTCCCCATCCCATCTCCTCTGTTTTCCTCTGCTTCTACAAAAATGGCTTCCATGGCTTCCACTCTCACCACCAAGCTCCAAACCCCCTCCTCCTCCTCCTCCTCCTTACTTGACTCCTCTTTCCATGGCACCCCTCTGGCCCCTCCTCCTTCTCTCCGCCTCAAACCCACCCCCGCCTCTTTCTCCATCTCTGCCTCCGCCTCTCAGCAGCAGCCTTACGATTTGAACTCCTTCAAATTCAACCCCATCAAGGAGTCCATCGTCTCACGTGAGATGACCCGCCGTTACATGACCGACATGATCACTTACGCCGATACTGATGTCGTCATCGTCGGCGCTGGCTCTGCTGGCCTCTCTTGCGCTTATGAACTTAGCAAAAACCCTGATATTCGCATCGCTATCATTGAGCAGTCCGTCTCCCCCGGCGGCGGTGCATGGCTCGGTGGCCAGCTCTTCTCTGCTATGGTGGTAAGGAAGCCAGCCCACTGCTTCTTGGACGAGGTTGGTGTGGAGTACGACGAGCAAGAGGACTACGTTGTAATTAAGCACGCGGCTCTGTTCACCTCAACGATCATGAGCAAGCTGCTGGCACGGCCCAATGTCAAGCTGTTCAACGCCGTGGCGGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGTGTCGTCACCAACTGGGCTTTAGTGTCAATGAACCATGACACACAGTCCTGCATGGACCCTAATGTCATGGAGGCGAAGGTAGTGGTCAGCTCTTGTGGCCACGACGGGCCCTTCGGAGCCACCGGAGTGAAGCGACTTAAGAGCATTGGCATGATCGATTCCGTCCCGGGAATGAAGGCCCTGGACATGAACACGGCGGAGGATGCCATTGTTAGGCTGACCCGAGAGGTGGTGCCTGGCATGATCGTAACGGGTATGGAGGTGGCGGAGATCGACGGAGCTCCAAGAATGGGGCCAACGTTCGGGGCAATGATGATATCGGGGCAGAAGGCGGCGCACCTGGCGTTGAAGTCATTGGGGCTGGCAAATTGCATTGAGGATGAAGCAGCTGATCTGATACTGGCGGCGGGAGAGTCACCGGAGGTTGCAGATGGTTGAATGGGTGAGTTTTCATGGATGGAAGGCGGCGGTTGTGTTTTGTGATTCATGGGGGCTCTGTTTCCGTGGCAAACAGATTGGGGTTTGGTGAAAAGCCAATTTTAAGTTAAATTAAATAAAATTGTATCAAAATGTTAATTGCCTTTTTCTTGTTAAATTATTATAAAATTAGATTTAAATTAATAATTTTGTAGTTAATAAA

Coding sequence (CDS)

ATGGCTTCCATGGCTTCCACTCTCACCACCAAGCTCCAAACCCCCTCCTCCTCCTCCTCCTCCTTACTTGACTCCTCTTTCCATGGCACCCCTCTGGCCCCTCCTCCTTCTCTCCGCCTCAAACCCACCCCCGCCTCTTTCTCCATCTCTGCCTCCGCCTCTCAGCAGCAGCCTTACGATTTGAACTCCTTCAAATTCAACCCCATCAAGGAGTCCATCGTCTCACGTGAGATGACCCGCCGTTACATGACCGACATGATCACTTACGCCGATACTGATGTCGTCATCGTCGGCGCTGGCTCTGCTGGCCTCTCTTGCGCTTATGAACTTAGCAAAAACCCTGATATTCGCATCGCTATCATTGAGCAGTCCGTCTCCCCCGGCGGCGGTGCATGGCTCGGTGGCCAGCTCTTCTCTGCTATGGTGGTAAGGAAGCCAGCCCACTGCTTCTTGGACGAGGTTGGTGTGGAGTACGACGAGCAAGAGGACTACGTTGTAATTAAGCACGCGGCTCTGTTCACCTCAACGATCATGAGCAAGCTGCTGGCACGGCCCAATGTCAAGCTGTTCAACGCCGTGGCGGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGTGTCGTCACCAACTGGGCTTTAGTGTCAATGAACCATGACACACAGTCCTGCATGGACCCTAATGTCATGGAGGCGAAGGTAGTGGTCAGCTCTTGTGGCCACGACGGGCCCTTCGGAGCCACCGGAGTGAAGCGACTTAAGAGCATTGGCATGATCGATTCCGTCCCGGGAATGAAGGCCCTGGACATGAACACGGCGGAGGATGCCATTGTTAGGCTGACCCGAGAGGTGGTGCCTGGCATGATCGTAACGGGTATGGAGGTGGCGGAGATCGACGGAGCTCCAAGAATGGGGCCAACGTTCGGGGCAATGATGATATCGGGGCAGAAGGCGGCGCACCTGGCGTTGAAGTCATTGGGGCTGGCAAATTGCATTGAGGATGAAGCAGCTGATCTGATACTGGCGGCGGGAGAGTCACCGGAGGTTGCAGATGGTTGA

Protein sequence

MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPTPASFSISASASQQQPYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG

Homology

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BLAST of Carg00054 vs. NCBI nr
Match: XP_022943248.1 (thiamine thiazole synthase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 642.9 bits (1657), Expect = 6.4e-181
Identity = 338/353 (95.75%), Postives = 340/353 (96.32%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLA-PPPSLRLKPTPASXXXXXXXXQQQPY 60
           MAS+ASTLTTKLQTP   SSSLLDSSFHGTPLA PPPSLRLK TPA+        QQQPY
Sbjct: 1   MASVASTLTTKLQTP---SSSLLDSSFHGTPLAPPPPSLRLKSTPAA--FSISASQQQPY 60

Query: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120
           DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA
Sbjct: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 353
           GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 348

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BLAST of Carg00054 vs. NCBI nr
Match: XP_023524627.1 (thiamine thiazole synthase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 621.7 bits (1602), Expect = 1.5e-174
Identity = 336/353 (95.18%), Postives = 336/353 (95.18%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPP-PSLRLKPTPASXXXXXXXXQQQPY 60
           MASMASTLTTKLQTP  SSSSLLDSSFHGTPLAPP            XXXXXXXX   PY
Sbjct: 1   MASMASTLTTKLQTP--SSSSLLDSSFHGTPLAPPXXXXXXXXXXXXXXXXXXXXXXXPY 60

Query: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120
           DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA
Sbjct: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 353
           GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 351

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BLAST of Carg00054 vs. NCBI nr
Match: XP_022990741.1 (thiamine thiazole synthase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 600.1 bits (1546), Expect = 4.8e-168
Identity = 317/355 (89.30%), Postives = 318/355 (89.58%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPS---SSSSSLLDSSFHGTPLAPPPSLRLKPTPASXXXXXXXXQQQ 60
           MASMAST TTKLQTPS                         RLK TPA+        Q Q
Sbjct: 1   MASMASTFTTKLQTPSXXXXXXXXXXXXXXXXXXXXXXXXXRLKSTPAAFSISASASQHQ 60

Query: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120
           PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR
Sbjct: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI
Sbjct: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG 353
           IDGAPRMGPTFGAMMISGQKAAHLALKSLGL NCIEDEAADLILAAGESPEVADG
Sbjct: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEAADLILAAGESPEVADG 355

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BLAST of Carg00054 vs. NCBI nr
Match: XP_004152916.1 (PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis sativus] >KGN56149.1 hypothetical protein Csa_3G078800 [Cucumis sativus])

HSP 1 Score: 592.4 bits (1526), Expect = 9.9e-166
Identity = 315/362 (87.02%), Postives = 322/362 (88.95%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPTPASXXXXXXXXQQQPYD 60
           MAS+ASTLTTKLQ P    S L DSSFHGTPLA P SLRLK T A+         Q PYD
Sbjct: 1   MASIASTLTTKLQKP----SLLHDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYD 60

Query: 61  LNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAI 120
           LN FKFNPI+ESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNP+IRIAI
Sbjct: 61  LNQFKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEA-----------ADLILAAGESPEV 352
           APRMGPTFGAMMISGQKAAHLALKSLG AN I DE             +L+LAAGESPEV
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEV 358

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BLAST of Carg00054 vs. NCBI nr
Match: XP_008463440.1 (PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo])

HSP 1 Score: 589.0 bits (1517), Expect = 1.1e-164
Identity = 312/362 (86.19%), Postives = 321/362 (88.67%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPTPASXXXXXXXXQQQPYD 60
           MAS+ASTLTTKLQ P    S L DSSFHGTPLA PPSLRLK   A+         Q PYD
Sbjct: 1   MASIASTLTTKLQIP----SLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYD 60

Query: 61  LNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAI 120
           LN FKFNPI+ESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNP+IRIAI
Sbjct: 61  LNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEA-----------ADLILAAGESPEV 352
           APRMGPTFGAMMISGQKAAHLALKSLG AN I  E             +L+LAAGESPEV
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEV 358

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BLAST of Carg00054 vs. TAIR10
Match: AT5G54770.1 (thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4))

HSP 1 Score: 515.4 bits (1326), Expect = 2.8e-146
Identity = 273/357 (76.47%), Postives = 302/357 (84.59%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPL-APPPSLRLKPTPASXXXXXXXXQQQPY 60
           MA++ASTL+      S+    L DSSFHG+ + A P S+ LKP   S            Y
Sbjct: 1   MAAIASTLSLS----STKPQRLFDSSFHGSAISAAPISIGLKPRSFSVRATTAG-----Y 60

Query: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120
           DLN+F F+PIKESIVSREMTRRYMTDMITYA+TDVV+VGAGSAGLS AYE+SKNP++++A
Sbjct: 61  DLNAFTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAM+VRKPAH FLDE+GV YDEQ+ YVV+KHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIGMID VPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIE-----DEAADLILAAGESPEVAD 352
           GAPRMGPTFGAMMISGQKA  LALK+LGL N I+     + + +L+LAA +S E  D
Sbjct: 301 GAPRMGPTFGAMMISGQKAGQLALKALGLPNAIDGTLVGNLSPELVLAAADSAETVD 348

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BLAST of Carg00054 vs. Swiss-Prot
Match: sp|O23787|THI4_CITSI (Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=2 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 2.3e-153
Identity = 288/358 (80.45%), Postives = 313/358 (87.43%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPTPAS--XXXXXXXXQQQP 60
           MA+MAST      + SS+++ L DSSFHG P++ P  LRL+P  +S             P
Sbjct: 1   MAAMASTAFA--PSVSSTTNKLFDSSFHGAPMS-PSLLRLQPIKSSRPNNLSISASASPP 60

Query: 61  YDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRI 120
           YDLN+FKF+PIKESIVSREMTRRYMTDMITYADTDVV+VGAGSAGLSCAYELSKNP+I+I
Sbjct: 61  YDLNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQI 120

Query: 121 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIM 180
           AIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G++YDEQ++YVVIKHAALFTSTIM
Sbjct: 121 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIM 180

Query: 181 SKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 240
           SKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS
Sbjct: 181 SKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 240

Query: 241 CGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEI 300
           CGHDGPFGATGVKRLKSIGMI+ VPGMKALDMN+AEDAIVRLTREVVPGMIVTGMEVAEI
Sbjct: 241 CGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEI 300

Query: 301 DGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAA-----DLILAAGESPEVAD 352
           DGAPRMGPTFGAMMISGQKAAHLALKSLG  N ++         +LILAA +S E AD
Sbjct: 301 DGAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDGTYVGGVHPELILAAADSAETAD 355

[Close]

BLAST of Carg00054 vs. Swiss-Prot
Match: sp|F6H7K5|THI42_VITVI (Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2 PE=3 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 4.9e-148
Identity = 283/359 (78.83%), Postives = 301/359 (83.84%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPTPASXXXXXXXXQQ-QPY 60
           MASMA+TLT+    P  +      SSFHG+P+    S R+ P   S            PY
Sbjct: 1   MASMATTLTSLSSNPKPAFFD-NKSSFHGSPI----SYRVLPIKVSHQSPTISMSSVSPY 60

Query: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120
           DL SFKF PIKESIV+REMTRRYM DMITYADTDVVIVGAGSAGLSCAYELSKNP IR+A
Sbjct: 61  DLQSFKFEPIKESIVAREMTRRYMMDMITYADTDVVIVGAGSAGLSCAYELSKNPSIRVA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHHFLDELGIEYDEQDNYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVK  RV GVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKEERVAGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTRE+VPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCI-------EDEAADLILAAGESPEVAD 352
           GAPRMGPTFGAMMISGQKAAHLAL++LG  N I       E    +LILAA E+ E+ D
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALRALGQPNAIDGNYTEAETMQPELILAAAETGEIVD 354

[Close]

BLAST of Carg00054 vs. Swiss-Prot
Match: sp|Q38709|THI4_ALNGL (Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=2 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 4.9e-148
Identity = 279/355 (78.59%), Postives = 306/355 (86.20%), Query Frame = 0


Query: 4   MASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPTP--ASXXXXXXXXQQQPYDL 63
           MASTLT+K Q  +   +S   SSF+GTPLA P S+R++PT   A            PYDL
Sbjct: 1   MASTLTSKPQRLALFENSA--SSFYGTPLA-PSSIRVQPTKAGAKPSISMSGAPSPPYDL 60

Query: 64  NSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAII 123
            +F F+PIKESIVSREMTRRYM DMITYADTDVV+VGAGS+GL C YELSKNP +++AII
Sbjct: 61  KAFTFDPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSSGLVC-YELSKNPSVQVAII 120

Query: 124 EQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKL 183
           EQSVSPGGGAWLGGQLFS MVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTIMSKL
Sbjct: 121 EQSVSPGGGAWLGGQLFSGMVVRKPAHLFLDELGIEYDEQDNYVVIKHAALFTSTIMSKL 180

Query: 184 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 243
           LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH
Sbjct: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240

Query: 244 DGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGA 303
           DGPFGATGVK L+SIGMID+VPGMKALDMN AEDAIVRLTRE+VPGMIVTGMEVAEIDGA
Sbjct: 241 DGPFGATGVKSLRSIGMIDTVPGMKALDMNVAEDAIVRLTREIVPGMIVTGMEVAEIDGA 300

Query: 304 PRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAA-----DLILAAGESPEVAD 352
           PRMGPTFGAMMISGQKAAHLALK+LGL N ++         +LILAA +S E+AD
Sbjct: 301 PRMGPTFGAMMISGQKAAHLALKALGLPNALDGSYVGGIHPELILAAADSAEIAD 351

[Close]

BLAST of Carg00054 vs. Swiss-Prot
Match: sp|F6H9A9|THI41_VITVI (Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 9.2e-147
Identity = 273/357 (76.47%), Postives = 305/357 (85.43%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPTPASXXXXXXXXQQQPYD 60
           MA++ S++ +K   P +S      SSFHG P+A     RL P  ++           PYD
Sbjct: 1   MATLTSSICSK---PKASVFDPHKSSFHGVPIA--TQARLSPVKSTPVNLAVTAAAMPYD 60

Query: 61  LNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAI 120
           L SFKF PIKESIVSREMTRRYM DMITYADTDVV+VGAGSAGLSCAYELSKNP +++AI
Sbjct: 61  LRSFKFEPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPSVQVAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFS+MVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSSMVVRKPAHRFLDELGLEYDEQDNYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLI+K G+VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIIKEGKVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRL+S+GMIDSVPGMKALDMNTAED IVRLTREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLRSVGMIDSVPGMKALDMNTAEDEIVRLTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGLANCIE-----DEAADLIL-AAGESPEVAD 352
           +PRMGPTFGAMMISGQKAAHLALKSLGL N ++     +   +L+L AA ++ E+A+
Sbjct: 301 SPRMGPTFGAMMISGQKAAHLALKSLGLPNALDGTYIGNLHPELVLAAAADAAEIAE 352

[Close]

BLAST of Carg00054 vs. Swiss-Prot
Match: sp|Q38814|THI4_ARATH (Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THI1 PE=1 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 5.0e-145
Identity = 273/357 (76.47%), Postives = 302/357 (84.59%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPL-APPPSLRLKPTPASXXXXXXXXQQQPY 60
           MA++ASTL+      S+    L DSSFHG+ + A P S+ LKP   S            Y
Sbjct: 1   MAAIASTLSLS----STKPQRLFDSSFHGSAISAAPISIGLKPRSFSVRATTAG-----Y 60

Query: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120
           DLN+F F+PIKESIVSREMTRRYMTDMITYA+TDVV+VGAGSAGLS AYE+SKNP++++A
Sbjct: 61  DLNAFTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAM+VRKPAH FLDE+GV YDEQ+ YVV+KHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIGMID VPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIE-----DEAADLILAAGESPEVAD 352
           GAPRMGPTFGAMMISGQKA  LALK+LGL N I+     + + +L+LAA +S E  D
Sbjct: 301 GAPRMGPTFGAMMISGQKAGQLALKALGLPNAIDGTLVGNLSPELVLAAADSAETVD 348

[Close]

BLAST of Carg00054 vs. TrEMBL
Match: tr|A0A0A0L546|A0A0A0L546_CUCSA (Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=3 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 6.6e-166
Identity = 315/362 (87.02%), Postives = 322/362 (88.95%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPTPASXXXXXXXXQQQPYD 60
           MAS+ASTLTTKLQ P    S L DSSFHGTPLA P SLRLK T A+         Q PYD
Sbjct: 1   MASIASTLTTKLQKP----SLLHDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYD 60

Query: 61  LNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAI 120
           LN FKFNPI+ESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNP+IRIAI
Sbjct: 61  LNQFKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEA-----------ADLILAAGESPEV 352
           APRMGPTFGAMMISGQKAAHLALKSLG AN I DE             +L+LAAGESPEV
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEV 358

[Close]

BLAST of Carg00054 vs. TrEMBL
Match: tr|A0A1S3CJ81|A0A1S3CJ81_CUCME (Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501605 PE=3 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 7.3e-165
Identity = 312/362 (86.19%), Postives = 321/362 (88.67%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPTPASXXXXXXXXQQQPYD 60
           MAS+ASTLTTKLQ P    S L DSSFHGTPLA PPSLRLK   A+         Q PYD
Sbjct: 1   MASIASTLTTKLQIP----SLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYD 60

Query: 61  LNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAI 120
           LN FKFNPI+ESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNP+IRIAI
Sbjct: 61  LNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEA-----------ADLILAAGESPEV 352
           APRMGPTFGAMMISGQKAAHLALKSLG AN I  E             +L+LAAGESPEV
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEV 358

[Close]

BLAST of Carg00054 vs. TrEMBL
Match: tr|A0A2I4EX88|A0A2I4EX88_9ROSI (Thiamine thiazole synthase, chloroplastic OS=Juglans regia OX=51240 GN=LOC108993518 PE=3 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 1.4e-152
Identity = 289/359 (80.50%), Postives = 314/359 (87.47%), Query Frame = 0


Query: 1   MASMASTLTTKLQ-TPSSSSSSLLDSSFHGTPLAPPPSLRLKP--TPASXXXXXXXXQQQ 60
           MA+MASTLT+K Q  P   S S   SSF+GTPLAPP S R++P  + A            
Sbjct: 1   MATMASTLTSKPQRLPLFESYS--SSSFYGTPLAPPSSFRVQPVKSGAHPPISMSASASP 60

Query: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120
           PYDL +FKF+PI+ESIVSREMTRRYM DMITYADTDVV+VGAGSAGLSCAYELSKNP ++
Sbjct: 61  PYDLKAFKFDPIRESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPSVQ 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           +AIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTI
Sbjct: 121 VAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDELGIEYDEQDNYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVME+KVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMESKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRL+SIGMIDSVPGMKALDMNTAEDAIVRLTRE+VPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLRSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAA-----DLILAAGESPEVAD 352
           IDG+PRMGPTFGAMMISGQKAAHLALKSLGL N ++         +LILA  ES E+AD
Sbjct: 301 IDGSPRMGPTFGAMMISGQKAAHLALKSLGLPNALDGSYVGSIHPELILAVAESAEIAD 357

[Close]

BLAST of Carg00054 vs. TrEMBL
Match: tr|A0A2P4JI82|A0A2P4JI82_QUESU (Thiamine thiazole synthase, chloroplastic OS=Quercus suber OX=58331 GN=THI1 PE=3 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 1.4e-152
Identity = 292/356 (82.02%), Postives = 313/356 (87.92%), Query Frame = 0


Query: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRL-KPTPASXXXXXXXXQQQ-- 60
           MA+MASTLT+K QT +   S    SSFHGTPLAPP SLR+ +P  +S             
Sbjct: 1   MATMASTLTSKPQTLAFFDS----SSFHGTPLAPPSSLRVHQPAKSSVAPHAISMSASPP 60

Query: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120
           PYDL  F F PIKESIVSREMTRRYMTDMITYADTDVV+VGAGSAGLSCAYELSKNP ++
Sbjct: 61  PYDLKGFAFEPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPSVQ 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           +AIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQ++YVVIKHAALFTSTI
Sbjct: 121 VAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDELEVEYDEQDNYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTRE+VPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEA--ADLILAAGESPEVAD 352
           IDGAPRMGPTFGAMMISGQKAAHLALK+LGL N ++  A   +LILAA +S E+A+
Sbjct: 301 IDGAPRMGPTFGAMMISGQKAAHLALKALGLPNALDAGAIQPELILAAADSAEIAE 352

[Close]

BLAST of Carg00054 vs. TrEMBL
Match: tr|W9S2E9|W9S2E9_9ROSA (Thiamine thiazole synthase, chloroplastic OS=Morus notabilis OX=981085 GN=THI1 PE=3 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 3.2e-152
Identity = 287/357 (80.39%), Postives = 314/357 (87.96%), Query Frame = 0


Query: 4   MASTLTTKLQTPSSSSSSLLD--SSFHGTPLA-PPPSLRLKPTPASXXXXXXXXQQQPYD 63
           MASTL+T   +  ++ +SL +  SSFHGTPLA PPP LRL+P  A            PYD
Sbjct: 1   MASTLST---SSFAAKTSLFESSSSFHGTPLAPPPPCLRLQPAKAG--FSVSMSAAAPYD 60

Query: 64  LNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAI 123
           L +F+F PIKESIVSREMTRRYM DMITYADTDVV+VGAGSAGLSCAYELSKNPD+++AI
Sbjct: 61  LKNFRFEPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPDVQVAI 120

Query: 124 IEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 183
           IEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIEYDEQDNYVVIKHAALFTSTIMSK 180

Query: 184 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 243
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCG 240

Query: 244 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 303
           HDGPFGATGVKRL+SIGMIDSVPGMKALDMN AEDAIVRLTRE+VPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLRSIGMIDSVPGMKALDMNAAEDAIVRLTREIVPGMIVTGMEVAEIDG 300

Query: 304 APRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAA------DLILAAGESPEVAD 352
           +PRMGPTFGAMMISGQKAAHLALKSLGL N ++  +       +LILAA E+ E+AD
Sbjct: 301 SPRMGPTFGAMMISGQKAAHLALKSLGLPNALDGSSVGSNIHPELILAAAENAEIAD 352

The following BLAST results are available for this feature:

BLAST of Carg00054 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022943248.1	6.4e-181	95.75	thiamine thiazole synthase, chloroplastic [Cucurbita moschata]	[more]
XP_023524627.1	1.5e-174	95.18	thiamine thiazole synthase, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_022990741.1	4.8e-168	89.30	thiamine thiazole synthase, chloroplastic [Cucurbita maxima]	[more]
XP_004152916.1	9.9e-166	87.02	PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis sativus] >KGN56149...	[more]
XP_008463440.1	1.1e-164	86.19	PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo]	[more]

BLAST of Carg00054 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G54770.1	2.8e-146	76.47	thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)	[more]

BLAST of Carg00054 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O23787\|THI4_CITSI	2.3e-153	80.45	Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=...	[more]
sp\|F6H7K5\|THI42_VITVI	4.9e-148	78.83	Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2...	[more]
sp\|Q38709\|THI4_ALNGL	4.9e-148	78.59	Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=...	[more]
sp\|F6H9A9\|THI41_VITVI	9.2e-147	76.47	Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1...	[more]
sp\|Q38814\|THI4_ARATH	5.0e-145	76.47	Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THI...	[more]

BLAST of Carg00054 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0L546\|A0A0A0L546_CUCSA	6.6e-166	87.02	Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=...	[more]
tr\|A0A1S3CJ81\|A0A1S3CJ81_CUCME	7.3e-165	86.19	Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10350160...	[more]
tr\|A0A2I4EX88\|A0A2I4EX88_9ROSI	1.4e-152	80.50	Thiamine thiazole synthase, chloroplastic OS=Juglans regia OX=51240 GN=LOC108993...	[more]
tr\|A0A2P4JI82\|A0A2P4JI82_QUESU	1.4e-152	82.02	Thiamine thiazole synthase, chloroplastic OS=Quercus suber OX=58331 GN=THI1 PE=3...	[more]
tr\|W9S2E9\|W9S2E9_9ROSA	3.2e-152	80.39	Thiamine thiazole synthase, chloroplastic OS=Morus notabilis OX=981085 GN=THI1 P...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0006950	response to stress
GO:0009228	thiamine biosynthetic process

Vocabulary: **INTERPRO**
Term	Definition
IPR027495	Sti35
IPR036188	FAD/NAD-bd_sf
IPR002922	Thi4_fam

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006950	response to stress
biological_process	GO:0009228	thiamine biosynthetic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg00054-RA	Carg00054-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002922	Thiazole biosynthetic enzyme Thi4 family	TIGRFAM	TIGR00292	TIGR00292	coord: 71..326 e-value: 7.0E-82 score: 272.4
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	G3DSA:3.50.50.60		coord: 91..326 e-value: 1.7E-113 score: 379.7
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	SSF51905	FAD/NAD(P)-binding domain	coord: 58..320
None	No IPR available	PFAM	PF01946	Thi4	coord: 75..308 e-value: 9.1E-115 score: 381.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..27
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..35
None	No IPR available	PANTHER	PTHR43422:SF3	THIAMINE THIAZOLE SYNTHASE	coord: 43..335
None	No IPR available	PANTHER	PTHR43422	FAMILY NOT NAMED	coord: 43..335
IPR027495	Thiamine thiazole synthase	HAMAP	MF_03158	THI4	coord: 5..335 score: 68.81

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg00054	Cla005283	Watermelon (97103) v1	carwmB0659
Carg00054	Csa3G078800	Cucumber (Chinese Long) v2	carcuB0684
Carg00054	MELO3C025408	Melon (DHL92) v3.5.1	carmeB0672
Carg00054	ClCG10G005650	Watermelon (Charleston Gray)	carwcgB0641
Carg00054	CSPI03G05330	Wild cucumber (PI 183967)	carcpiB0696
Carg00054	Cucsa.059760	Cucumber (Gy14) v1	carcgyB0738
Carg00054	CmaCh04G009410	Cucurbita maxima (Rimu)	carcmaB0948
Carg00054	CmaCh16G008490	Cucurbita maxima (Rimu)	carcmaB0946
Carg00054	CmoCh04G010110	Cucurbita moschata (Rifu)	carcmoB0923
Carg00054	CmoCh16G009100	Cucurbita moschata (Rifu)	carcmoB0921
Carg00054	Lsi05G006220	Bottle gourd (USVL1VR-Ls)	carlsiB616
Carg00054	Cp4.1LG00g00120	Cucurbita pepo (Zucchini)	carcpeB0933
Carg00054	Cp4.1LG01g08250	Cucurbita pepo (Zucchini)	carcpeB0938
Carg00054	CsGy3G005410	Cucumber (Gy14) v2	carcgybB0659
Carg00054	MELO3C025408.2	Melon (DHL92) v3.6.1	carmedB0684
Carg00054	CsaV3_3G005340	Cucumber (Chinese Long) v3	carcucB0698
Carg00054	Cla97C10G189870	Watermelon (97103) v2	carwmbB0686
Carg00054	Bhi11G000513	Wax gourd	carwgoB0777

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg00054	Carg24821	Silver-seed gourd	carcarB567

The following block(s) are covering this gene:


Gene	Organism	Block
Carg00054	Cucumber (Gy14) v2	carcgybB0662
Carg00054	Cucurbita moschata (Rifu)	carcmoB0920
Carg00054	Bottle gourd (USVL1VR-Ls)	carlsiB613
Carg00054	Bottle gourd (USVL1VR-Ls)	carlsiB615
Carg00054	Melon (DHL92) v3.5.1	carmeB0670