Bhi11G000513 (gene) Wax gourd

Overview
NameBhi11G000513
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionThiamine thiazole synthase protein
Locationchr11 : 15098491 .. 15100483 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAAAATTGTGCGACATTCTTCGATATCTTCTATAAAACCCTTGTTCCTTCCCCTCATCTTCCCCACACAGCCTTCTCAAACTACATCTTCTCTGTTTTCCTCTGCTTCAAAAATGGCTTCCATTGCCTCCACTCTCACCACCAAGCTCCAAAGGCCTTCCTTACTTCTTGACTCGTCTTTCAATGGCACCCCTTTGGCCCCTCCTCCTTCTCTCCGCCTCAAATCCACCACCACCACCACCGCCGCCACATCTCTCTCCATCTCCGCCTCTGCCTCTCAGCCCCCCTACGATTTGAACCAATTCAAATTCAATCCCATCAAGGAATCCATCGTCTCCCGAGAGATGACCCGTCGCTACATGACTGACATGATTACCTACGCTGACACTGACGTGATCATCGTCGGCGCTGGCTCCGCCGGCCTCTCTTGCGCTTACGAGCTCAGCAAAAACCCATCTATTCGTATCGCCATCATCGAACAATCCGTCAGCCCCGGCGGCGGTGCATGGCTCGGCGGCCAACTCTTCTCTGCTATGGTAGGAATTTCATTTCCTTTCCATATATGCCTTACCATTTTCTTACCTTCTCCTCATTTGGATAGATGCTCTGGTTTTTTGTTTTTTTCTTGAAAATACCAAAAATTTCATAATCCTCCACTCAGATTTCATGCATGCCTTATTTGGACTCAAGGTTTCATCATTTTGAACAGAGATCTGAAAAAAGGAAAGATGTTGAACCAAATATATTCTTGACGATTCTGGGAAATAGTATTCAGTTCTTGTGATGCTCTGAGATTAAATTTCATTAAGAAATTTGAACAAAGATTCTGATCTTGTCAATCAAATAGATTTGGGTTAAATTTAATTTTTGATTAAAATTGTTTGACAGGTGGTTCGGAAACCAGCCCATTTGTTCTTGGACGAGGTGGGTGTGGAGTACGACGAGCAAGAAGACTACGTCGTGATAAAGCACGCGGCTCTCTTCACGTCAACGATCATGAGCAAGCTCCTCGCCCGGCCGAACGTGAAGCTGTTCAATGCGGTGGCGGCGGAGGATTTGATCGTTAAAGGCGGCAGAGTTGGCGGTGTCGTCACCAACTGGGCGTTGGTGTCGATGAACCATGACACACAGTCCTGCATGGACCCCAATGTGATGGAGGCCAAGGTGGTTGTGAGTTCTTGTGGCCACGACGGACCCTTCGGCGCTACCGGAGTCAAGCGCCTCAAGAGCATCGGCTTGATCGATAGTGTCCCTGGGATGAAGGCGCTTGACATGAACACTGCCGAGGATGCCATTGTTCGGTTCACCCGAGAGGTCGTTCCCGGCATGATTGTCACCGGCATGGAGGTTGCCGAGATCGATGGAGCTCCAAGAATGGTAAACCAACTAGAGATTACTGCTATAGAACAAAATTTAGATACAGCTGTTGGAATCTATCGTGTGATCAACGATAGAGTATAAATTTTACTATATTCATCGTTCTTTAAAAATGTTGCTAGCTATTACCCAAATGTACTATGAGTTGGATAAGCTTAAGTTAATGGGTTATATAATATATCTGACGTGCGATGCAGGGGCCGACGTTCGGGGCGATGATGATATCGGGGCAGAAGGCGGCACACTTGGCGCTGAAATCGTTGGGGGAGGCGAATGGCATTGAGGATGGAGAAGTGGGAGAAAGAAAGGTTGTGGAAGAACCAGAGCTGCTACTGGCGGCGGGGGAGTCGCCGGAGGTTGCAGATGCTTGAAATTTTTAATGACGGCCGGATGTTGATTTGGATGAAATATTTGCCTTTGGGTTTATGATGTTTGTTTGTTAGTAGTGAGTTAAGAGTGTGATGAAAGGGGTTGTGTTTGGCTTTGTGGTTTCATAGCACAATGGGGTCTGGGGGAAGACAAAATATCAATTCCCTTTTTCTCTCTTTTTTTTGTTTTACTTTTATTTTGTTAATAAAAATGCCTTTCCAAACCATCTCTTCCCCACAAAAATGAG

mRNA sequence

GTAAAATTGTGCGACATTCTTCGATATCTTCTATAAAACCCTTGTTCCTTCCCCTCATCTTCCCCACACAGCCTTCTCAAACTACATCTTCTCTGTTTTCCTCTGCTTCAAAAATGGCTTCCATTGCCTCCACTCTCACCACCAAGCTCCAAAGGCCTTCCTTACTTCTTGACTCGTCTTTCAATGGCACCCCTTTGGCCCCTCCTCCTTCTCTCCGCCTCAAATCCACCACCACCACCACCGCCGCCACATCTCTCTCCATCTCCGCCTCTGCCTCTCAGCCCCCCTACGATTTGAACCAATTCAAATTCAATCCCATCAAGGAATCCATCGTCTCCCGAGAGATGACCCGTCGCTACATGACTGACATGATTACCTACGCTGACACTGACGTGATCATCGTCGGCGCTGGCTCCGCCGGCCTCTCTTGCGCTTACGAGCTCAGCAAAAACCCATCTATTCGTATCGCCATCATCGAACAATCCGTCAGCCCCGGCGGCGGTGCATGGCTCGGCGGCCAACTCTTCTCTGCTATGGTGGTTCGGAAACCAGCCCATTTGTTCTTGGACGAGGTGGGTGTGGAGTACGACGAGCAAGAAGACTACGTCGTGATAAAGCACGCGGCTCTCTTCACGTCAACGATCATGAGCAAGCTCCTCGCCCGGCCGAACGTGAAGCTGTTCAATGCGGTGGCGGCGGAGGATTTGATCGTTAAAGGCGGCAGAGTTGGCGGTGTCGTCACCAACTGGGCGTTGGTGTCGATGAACCATGACACACAGTCCTGCATGGACCCCAATGTGATGGAGGCCAAGGTGGTTGTGAGTTCTTGTGGCCACGACGGACCCTTCGGCGCTACCGGAGTCAAGCGCCTCAAGAGCATCGGCTTGATCGATAGTGTCCCTGGGATGAAGGCGCTTGACATGAACACTGCCGAGGATGCCATTGTTCGGTTCACCCGAGAGGTCGTTCCCGGCATGATTGTCACCGGCATGGAGGTTGCCGAGATCGATGGAGCTCCAAGAATGGGGCCGACGTTCGGGGCGATGATGATATCGGGGCAGAAGGCGGCACACTTGGCGCTGAAATCGTTGGGGGAGGCGAATGGCATTGAGGATGGAGAAGTGGGAGAAAGAAAGGTTGTGGAAGAACCAGAGCTGCTACTGGCGGCGGGGGAGTCGCCGGAGGTTGCAGATGCTTGAAATTTTTAATGACGGCCGGATGTTGATTTGGATGAAATATTTGCCTTTGGGTTTATGATGTTTGTTTGTTAGTAGTGAGTTAAGAGTGTGATGAAAGGGGTTGTGTTTGGCTTTGTGGTTTCATAGCACAATGGGGTCTGGGGGAAGACAAAATATCAATTCCCTTTTTCTCTCTTTTTTTTGTTTTACTTTTATTTTGTTAATAAAAATGCCTTTCCAAACCATCTCTTCCCCACAAAAATGAG

Coding sequence (CDS)

ATGGCTTCCATTGCCTCCACTCTCACCACCAAGCTCCAAAGGCCTTCCTTACTTCTTGACTCGTCTTTCAATGGCACCCCTTTGGCCCCTCCTCCTTCTCTCCGCCTCAAATCCACCACCACCACCACCGCCGCCACATCTCTCTCCATCTCCGCCTCTGCCTCTCAGCCCCCCTACGATTTGAACCAATTCAAATTCAATCCCATCAAGGAATCCATCGTCTCCCGAGAGATGACCCGTCGCTACATGACTGACATGATTACCTACGCTGACACTGACGTGATCATCGTCGGCGCTGGCTCCGCCGGCCTCTCTTGCGCTTACGAGCTCAGCAAAAACCCATCTATTCGTATCGCCATCATCGAACAATCCGTCAGCCCCGGCGGCGGTGCATGGCTCGGCGGCCAACTCTTCTCTGCTATGGTGGTTCGGAAACCAGCCCATTTGTTCTTGGACGAGGTGGGTGTGGAGTACGACGAGCAAGAAGACTACGTCGTGATAAAGCACGCGGCTCTCTTCACGTCAACGATCATGAGCAAGCTCCTCGCCCGGCCGAACGTGAAGCTGTTCAATGCGGTGGCGGCGGAGGATTTGATCGTTAAAGGCGGCAGAGTTGGCGGTGTCGTCACCAACTGGGCGTTGGTGTCGATGAACCATGACACACAGTCCTGCATGGACCCCAATGTGATGGAGGCCAAGGTGGTTGTGAGTTCTTGTGGCCACGACGGACCCTTCGGCGCTACCGGAGTCAAGCGCCTCAAGAGCATCGGCTTGATCGATAGTGTCCCTGGGATGAAGGCGCTTGACATGAACACTGCCGAGGATGCCATTGTTCGGTTCACCCGAGAGGTCGTTCCCGGCATGATTGTCACCGGCATGGAGGTTGCCGAGATCGATGGAGCTCCAAGAATGGGGCCGACGTTCGGGGCGATGATGATATCGGGGCAGAAGGCGGCACACTTGGCGCTGAAATCGTTGGGGGAGGCGAATGGCATTGAGGATGGAGAAGTGGGAGAAAGAAAGGTTGTGGAAGAACCAGAGCTGCTACTGGCGGCGGGGGAGTCGCCGGAGGTTGCAGATGCTTGA

Protein sequence

MASIASTLTTKLQRPSLLLDSSFNGTPLAPPPSLRLKSTTTTTAATSLSISASASQPPYDLNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGEANGIEDGEVGERKVVEEPELLLAAGESPEVADA
Homology
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BLAST of Bhi11G000513 vs. TAIR10
Match: AT5G54770.1 (thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4))

HSP 1 Score: 516.5 bits (1329), Expect = 1.3e-146
Identity = 269/361 (74.52%), Postives = 296/361 (81.99%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLLDSSFNGTPLAPPPSXXXXXXXXXXXXXXXXXXXXXXXPPYD 60
           MA+IASTL+    +P  L DSSF+G+ ++  P                          YD
Sbjct: 1   MAAIASTLSLSSTKPQRLFDSSFHGSAISAAP--------ISIGLKPRSFSVRATTAGYD 60

Query: 61  LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAI 120
           LN F F+PIKESIVSREMTRRYMTDMITYA+TDV++VGAGSAGLS AYE+SKNP++++AI
Sbjct: 61  LNAFTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAM+VRKPAHLFLDE+GV YDEQ+ YVV+KHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIG+ID VPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANGIEDGEVGERKVVEEPELLLAAGESPEVAD 360
           APRMGPTFGAMMISGQKA  LALK+LG  N I+   VG       PEL+LAA +S E  D
Sbjct: 301 APRMGPTFGAMMISGQKAGQLALKALGLPNAIDGTLVGNL----SPELVLAAADSAETVD 349

Query: 361 A 362
           A
Sbjct: 361 A 349

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BLAST of Bhi11G000513 vs. Swiss-Prot
Match: sp|O23787|THI4_CITSI (Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=2 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 9.7e-152
Identity = 281/363 (77.41%), Postives = 307/363 (84.57%), Query Frame = 0 

Query: 1   MASIAST--LTTKLQRPSLLLDSSFNGTPLAPPPSXXXXXXXXXXXXXXXXXXXXXXXPP 60
           MA++AST    +     + L DSSF+G P++P                          PP
Sbjct: 1   MAAMASTAFAPSVSSTTNKLFDSSFHGAPMSP---SLLRLQPIKSSRPNNLSISASASPP 60

Query: 61  YDLNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRI 120
           YDLN FKF+PIKESIVSREMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSKNP+I+I
Sbjct: 61  YDLNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQI 120

Query: 121 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIM 180
           AIIEQSVSPGGGAWLGGQLFSAMVVRKPAH+FLDE+G++YDEQ++YVVIKHAALFTSTIM
Sbjct: 121 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIM 180

Query: 181 SKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 240
           SKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS
Sbjct: 181 SKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 240

Query: 241 CGHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEI 300
           CGHDGPFGATGVKRLKSIG+I+ VPGMKALDMN+AEDAIVR TREVVPGMIVTGMEVAEI
Sbjct: 241 CGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEI 300

Query: 301 DGAPRMGPTFGAMMISGQKAAHLALKSLGEANGIEDGEVGERKVVEEPELLLAAGESPEV 360
           DGAPRMGPTFGAMMISGQKAAHLALKSLG+ N ++   VG       PEL+LAA +S E 
Sbjct: 301 DGAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDGTYVGG----VHPELILAAADSAET 356

Query: 361 ADA 362
           ADA
Sbjct: 361 ADA 356

[Close]
BLAST of Bhi11G000513 vs. Swiss-Prot
Match: sp|F6H7K5|THI42_VITVI (Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2 PE=3 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 2.9e-148
Identity = 278/364 (76.37%), Postives = 303/364 (83.24%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLL---DSSFNGTPLAPPPSXXXXXXXXXXXXXXXXXXXXXXXP 60
           MAS+A+TLT+    P        SSF+G+P++                            
Sbjct: 1   MASMATTLTSLSSNPKPAFFDNKSSFHGSPIS-------YRVLPIKVSHQSPTISMSSVS 60

Query: 61  PYDLNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIR 120
           PYDL  FKF PIKESIV+REMTRRYM DMITYADTDV+IVGAGSAGLSCAYELSKNPSIR
Sbjct: 61  PYDLQSFKFEPIKESIVAREMTRRYMMDMITYADTDVVIVGAGSAGLSCAYELSKNPSIR 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           +AIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTI
Sbjct: 121 VAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHHFLDELGIEYDEQDNYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLIVK  RV GVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKEERVAGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TRE+VPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGEANGIEDGEVGERKVVEEPELLLAAGESPE 360
           IDGAPRMGPTFGAMMISGQKAAHLAL++LG+ N I DG   E + + +PEL+LAA E+ E
Sbjct: 301 IDGAPRMGPTFGAMMISGQKAAHLALRALGQPNAI-DGNYTEAETM-QPELILAAAETGE 355

Query: 361 VADA 362
           + DA
Sbjct: 361 IVDA 355

[Close]
BLAST of Bhi11G000513 vs. Swiss-Prot
Match: sp|Q38709|THI4_ALNGL (Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=2 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 6.5e-148
Identity = 277/360 (76.94%), Postives = 302/360 (83.89%), Query Frame = 0 

Query: 4   IASTLTTKLQRPSLLLD--SSFNGTPLAPPPSXXXXXXXXXXXXXXXXXXXXXXXPPYDL 63
           +ASTLT+K QR +L  +  SSF GTPLAP                          PPYDL
Sbjct: 1   MASTLTSKPQRLALFENSASSFYGTPLAP---SSIRVQPTKAGAKPSISMSGAPSPPYDL 60

Query: 64  NQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAII 123
             F F+PIKESIVSREMTRRYM DMITYADTDV++VGAGS+GL C YELSKNPS+++AII
Sbjct: 61  KAFTFDPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSSGLVC-YELSKNPSVQVAII 120

Query: 124 EQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKL 183
           EQSVSPGGGAWLGGQLFS MVVRKPAHLFLDE+G+EYDEQ++YVVIKHAALFTSTIMSKL
Sbjct: 121 EQSVSPGGGAWLGGQLFSGMVVRKPAHLFLDELGIEYDEQDNYVVIKHAALFTSTIMSKL 180

Query: 184 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 243
           LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH
Sbjct: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240

Query: 244 DGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA 303
           DGPFGATGVK L+SIG+ID+VPGMKALDMN AEDAIVR TRE+VPGMIVTGMEVAEIDGA
Sbjct: 241 DGPFGATGVKSLRSIGMIDTVPGMKALDMNVAEDAIVRLTREIVPGMIVTGMEVAEIDGA 300

Query: 304 PRMGPTFGAMMISGQKAAHLALKSLGEANGIEDGEVGERKVVEEPELLLAAGESPEVADA 362
           PRMGPTFGAMMISGQKAAHLALK+LG  N ++   VG       PEL+LAA +S E+ADA
Sbjct: 301 PRMGPTFGAMMISGQKAAHLALKALGLPNALDGSYVGG----IHPELILAAADSAEIADA 352

[Close]
BLAST of Bhi11G000513 vs. Swiss-Prot
Match: sp|Q38814|THI4_ARATH (Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THI1 PE=1 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 2.3e-145
Identity = 269/361 (74.52%), Postives = 296/361 (81.99%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLLDSSFNGTPLAPPPSXXXXXXXXXXXXXXXXXXXXXXXPPYD 60
           MA+IASTL+    +P  L DSSF+G+ ++  P                          YD
Sbjct: 1   MAAIASTLSLSSTKPQRLFDSSFHGSAISAAP--------ISIGLKPRSFSVRATTAGYD 60

Query: 61  LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAI 120
           LN F F+PIKESIVSREMTRRYMTDMITYA+TDV++VGAGSAGLS AYE+SKNP++++AI
Sbjct: 61  LNAFTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAM+VRKPAHLFLDE+GV YDEQ+ YVV+KHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIG+ID VPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANGIEDGEVGERKVVEEPELLLAAGESPEVAD 360
           APRMGPTFGAMMISGQKA  LALK+LG  N I+   VG       PEL+LAA +S E  D
Sbjct: 301 APRMGPTFGAMMISGQKAGQLALKALGLPNAIDGTLVGNL----SPELVLAAADSAETVD 349

Query: 361 A 362
           A
Sbjct: 361 A 349

[Close]
BLAST of Bhi11G000513 vs. Swiss-Prot
Match: sp|F6H9A9|THI41_VITVI (Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1 PE=3 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 2.6e-144
Identity = 257/305 (84.26%), Postives = 281/305 (92.13%), Query Frame = 0 

Query: 58  PYDLNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIR 117
           PYDL  FKF PIKESIVSREMTRRYM DMITYADTDV++VGAGSAGLSCAYELSKNPS++
Sbjct: 53  PYDLRSFKFEPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPSVQ 112

Query: 118 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTI 177
           +AIIEQSVSPGGGAWLGGQLFS+MVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTI
Sbjct: 113 VAIIEQSVSPGGGAWLGGQLFSSMVVRKPAHRFLDELGLEYDEQDNYVVIKHAALFTSTI 172

Query: 178 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 237
           MSKLLARPNVKLFNAVAAEDLI+K G+VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 173 MSKLLARPNVKLFNAVAAEDLIIKEGKVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 232

Query: 238 SCGHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAE 297
           SCGHDGPFGATGVKRL+S+G+IDSVPGMKALDMNTAED IVR TREVVPGMIVTGMEVAE
Sbjct: 233 SCGHDGPFGATGVKRLRSVGMIDSVPGMKALDMNTAEDEIVRLTREVVPGMIVTGMEVAE 292

Query: 298 IDGAPRMGPTFGAMMISGQKAAHLALKSLGEANGIEDGEVGERKVVEEPELLL-AAGESP 357
           IDG+PRMGPTFGAMMISGQKAAHLALKSLG  N ++   +G       PEL+L AA ++ 
Sbjct: 293 IDGSPRMGPTFGAMMISGQKAAHLALKSLGLPNALDGTYIGNL----HPELVLAAAADAA 352

Query: 358 EVADA 362
           E+A+A
Sbjct: 353 EIAEA 353

[Close]
BLAST of Bhi11G000513 vs. TrEMBL
Match: tr|A0A1S3CJ81|A0A1S3CJ81_CUCME (Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501605 PE=3 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 4.8e-172
Identity = 325/363 (89.53%), Postives = 332/363 (91.46%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLLDSSFNGTPLAPPPSXXXXXXXXXXXXXXXXXXXXXXXPPYD 60
           MASIASTLTTKLQ PSLL DSSF+GTPLA PPS                       PPYD
Sbjct: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPS----LRLKSPAATSLTISASASQPPYD 60

Query: 61  LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAI 120
           LNQFKFNPI+ESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNP+IRIAI
Sbjct: 61  LNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQ+DYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANGI-EDGEVGERKV-VEEPELLLAAGESPEV 360
           APRMGPTFGAMMISGQKAAHLALKSLGEAN I  +GE+GERKV +EE ELLLAAGESPEV
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEV 359

Query: 361 ADA 362
           ADA
Sbjct: 361 ADA 359

[Close]
BLAST of Bhi11G000513 vs. TrEMBL
Match: tr|A0A0A0L546|A0A0A0L546_CUCSA (Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=3 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 8.2e-172
Identity = 322/363 (88.71%), Postives = 333/363 (91.74%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLLDSSFNGTPLAPPPSXXXXXXXXXXXXXXXXXXXXXXXPPYD 60
           MASIASTLTTKLQ+PSLL DSSF+GTPLA P S                       PPYD
Sbjct: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSS----LRLKSTAATSLTISASASQPPYD 60

Query: 61  LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAI 120
           LNQFKFNPI+ESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNP+IRIAI
Sbjct: 61  LNQFKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQ+DYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANGI-EDGEVGERKV-VEEPELLLAAGESPEV 360
           APRMGPTFGAMMISGQKAAHLALKSLGEAN I ++GE+GERKV +EE ELLLAAGESPEV
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEV 359

Query: 361 ADA 362
           ADA
Sbjct: 361 ADA 359

[Close]
BLAST of Bhi11G000513 vs. TrEMBL
Match: tr|A0A2I4EX88|A0A2I4EX88_9ROSI (Thiamine thiazole synthase, chloroplastic OS=Juglans regia OX=51240 GN=LOC108993518 PE=3 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 8.5e-153
Identity = 287/364 (78.85%), Postives = 310/364 (85.16%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLL---LDSSFNGTPLAPPPSXXXXXXXXXXXXXXXXXXXXXXXP 60
           MA++ASTLT+K QR  L      SSF GTPLAPP S                       P
Sbjct: 1   MATMASTLTSKPQRLPLFESYSSSSFYGTPLAPPSS--FRVQPVKSGAHPPISMSASASP 60

Query: 61  PYDLNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIR 120
           PYDL  FKF+PI+ESIVSREMTRRYM DMITYADTDV++VGAGSAGLSCAYELSKNPS++
Sbjct: 61  PYDLKAFKFDPIRESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPSVQ 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           +AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDE+G+EYDEQ++YVVIKHAALFTSTI
Sbjct: 121 VAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDELGIEYDEQDNYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVME+KVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMESKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRL+SIG+IDSVPGMKALDMNTAEDAIVR TRE+VPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLRSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGEANGIEDGEVGERKVVEEPELLLAAGESPE 360
           IDG+PRMGPTFGAMMISGQKAAHLALKSLG  N ++   VG       PEL+LA  ES E
Sbjct: 301 IDGSPRMGPTFGAMMISGQKAAHLALKSLGLPNALDGSYVGS----IHPELILAVAESAE 358

Query: 361 VADA 362
           +ADA
Sbjct: 361 IADA 358

[Close]
BLAST of Bhi11G000513 vs. TrEMBL
Match: tr|A0A2P4JI82|A0A2P4JI82_QUESU (Thiamine thiazole synthase, chloroplastic OS=Quercus suber OX=58331 GN=THI1 PE=3 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 1.1e-152
Identity = 285/361 (78.95%), Postives = 309/361 (85.60%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLLDSSFNGTPLAPPPSXXXXXXXXXXXXXXXXXXXXXXXPPYD 60
           MA++ASTLT+K Q  +    SSF+GTPLA PPS                       PPYD
Sbjct: 1   MATMASTLTSKPQTLAFFDSSSFHGTPLA-PPSSLRVHQPAKSSVAPHAISMSASPPPYD 60

Query: 61  LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAI 120
           L  F F PIKESIVSREMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSKNPS+++AI
Sbjct: 61  LKGFAFEPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPSVQVAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDE+ VEYDEQ++YVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDELEVEYDEQDNYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TRE+VPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANGIEDGEVGERKVVEEPELLLAAGESPEVAD 360
           APRMGPTFGAMMISGQKAAHLALK+LG  N ++ G +       +PEL+LAA +S E+A+
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKALGLPNALDAGAI-------QPELILAAADSAEIAE 353

Query: 361 A 362
           A
Sbjct: 361 A 353

[Close]
BLAST of Bhi11G000513 vs. TrEMBL
Match: tr|A0A061F760|A0A061F760_THECC (Thiamine thiazole synthase, chloroplastic OS=Theobroma cacao OX=3641 GN=THI1 PE=3 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 8.0e-151
Identity = 281/362 (77.62%), Postives = 308/362 (85.08%), Query Frame = 0 

Query: 2   ASIASTLT--TKLQRPSLLLDSSFNGTPLAPPPSXXXXXXXXXXXXXXXXXXXXXXXPPY 61
           +S+A+TLT  TKL R + L +SSF+G P+   P                        PPY
Sbjct: 3   SSVATTLTSSTKLYRNTSLFESSFHGVPIT--PLSFHLKAKSSPRNASVSMSAASSPPPY 62

Query: 62  DLNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIA 121
           DLN F+F+PIKESIVSREMTRRYM DMIT+ADTDV++VGAGSAGLSCAYELSKNPS+++A
Sbjct: 63  DLNNFRFDPIKESIVSREMTRRYMMDMITHADTDVVVVGAGSAGLSCAYELSKNPSVQVA 122

Query: 122 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 181
           I+EQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQ+DYVVIKHAALFTSTIMS
Sbjct: 123 IVEQSVSPGGGAWLGGQLFSAMVVRKPAHRFLDELGIEYDEQDDYVVIKHAALFTSTIMS 182

Query: 182 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 241
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 183 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 242

Query: 242 GHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEID 301
           GHDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TRE+VPGMIVTGMEVAEID
Sbjct: 243 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEID 302

Query: 302 GAPRMGPTFGAMMISGQKAAHLALKSLGEANGIEDGEVGERKVVEEPELLLAAGESPEVA 361
           G+PRMGPTFGAMMISGQKAAHLAL+SLG  N ++   VG       PEL+LAA +S E A
Sbjct: 303 GSPRMGPTFGAMMISGQKAAHLALQSLGLPNALDGTYVGN----IHPELILAAADSAETA 358

[Close]
BLAST of Bhi11G000513 vs. NCBI nr
Match: XP_008463440.1 (PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo])

HSP 1 Score: 612.8 bits (1579), Expect = 7.3e-172
Identity = 325/363 (89.53%), Postives = 332/363 (91.46%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLLDSSFNGTPLAPPPSXXXXXXXXXXXXXXXXXXXXXXXPPYD 60
           MASIASTLTTKLQ PSLL DSSF+GTPLA PPS                       PPYD
Sbjct: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPS----LRLKSPAATSLTISASASQPPYD 60

Query: 61  LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAI 120
           LNQFKFNPI+ESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNP+IRIAI
Sbjct: 61  LNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQ+DYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANGI-EDGEVGERKV-VEEPELLLAAGESPEV 360
           APRMGPTFGAMMISGQKAAHLALKSLGEAN I  +GE+GERKV +EE ELLLAAGESPEV
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEV 359

Query: 361 ADA 362
           ADA
Sbjct: 361 ADA 359

[Close]
BLAST of Bhi11G000513 vs. NCBI nr
Match: XP_004152916.1 (PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis sativus] >KGN56149.1 hypothetical protein Csa_3G078800 [Cucumis sativus])

HSP 1 Score: 612.1 bits (1577), Expect = 1.2e-171
Identity = 322/363 (88.71%), Postives = 333/363 (91.74%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLLDSSFNGTPLAPPPSXXXXXXXXXXXXXXXXXXXXXXXPPYD 60
           MASIASTLTTKLQ+PSLL DSSF+GTPLA P S                       PPYD
Sbjct: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSS----LRLKSTAATSLTISASASQPPYD 60

Query: 61  LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAI 120
           LNQFKFNPI+ESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNP+IRIAI
Sbjct: 61  LNQFKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQ+DYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANGI-EDGEVGERKV-VEEPELLLAAGESPEV 360
           APRMGPTFGAMMISGQKAAHLALKSLGEAN I ++GE+GERKV +EE ELLLAAGESPEV
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEV 359

Query: 361 ADA 362
           ADA
Sbjct: 361 ADA 359

[Close]
BLAST of Bhi11G000513 vs. NCBI nr
Match: XP_022943248.1 (thiamine thiazole synthase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 583.6 bits (1503), Expect = 4.7e-163
Identity = 310/361 (85.87%), Postives = 316/361 (87.53%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRP-SLLLDSSFNGTPLAPPPSXXXXXXXXXXXXXXXXXXXXXXXPPY 60
           MAS+ASTLTTKLQ P S LLDSSF+GTPLAPPP                         PY
Sbjct: 1   MASVASTLTTKLQTPSSSLLDSSFHGTPLAPPP-----PSLRLKSTPAAFSISASQQQPY 60

Query: 61  DLNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIA 120
           DLN FKFNPIKESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNP IRIA
Sbjct: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQEDYVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGEANGIEDGEVGERKVVEEPELLLAAGESPEVA 360
           GAPRMGPTFGAMMISGQKAAHLALKSLG AN IED         E  +L+LAAGESPEVA
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIED---------EAADLILAAGESPEVA 347

[Close]
BLAST of Bhi11G000513 vs. NCBI nr
Match: XP_022985241.1 (thiamine thiazole synthase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 581.3 bits (1497), Expect = 2.3e-162
Identity = 308/362 (85.08%), Postives = 323/362 (89.23%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRPSLLLDSSFNGTPLAPPPSXXXXXXXXXXXXXXXXXXXXXXXPPYD 60
           MASIAS+L TKL RPS LLDSSF+G+PL P PS                       PPYD
Sbjct: 1   MASIASSLPTKLHRPS-LLDSSFHGSPLLPTPS---FRLKPTTAAASLSISASAAQPPYD 60

Query: 61  LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAI 120
           LNQFKFNPI+ESIVSREMTRRYMTDMITYADTDVI+VGAGSAGLSCAYELSK+PS+R+AI
Sbjct: 61  LNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSVRVAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQEDYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANGI-EDGEVGERKVVEEPELLLAAGESPEVA 360
           APRMGPTFGAMMISGQKAAHLALK+LG+AN I E GE G RK VE  E++LAAGE+PEVA
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKALGKANSIDEKGENGGRK-VEAAEMVLAAGETPEVA 357

Query: 361 DA 362
           DA
Sbjct: 361 DA 357

[Close]
BLAST of Bhi11G000513 vs. NCBI nr
Match: XP_023524627.1 (thiamine thiazole synthase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 579.3 bits (1492), Expect = 8.9e-162
Identity = 331/362 (91.44%), Postives = 337/362 (93.09%), Query Frame = 0 

Query: 1   MASIASTLTTKLQRP--SLLLDSSFNGTPLAPPPSXXXXXXXXXXXXXXXXXXXXXXXPP 60
           MAS+ASTLTTKLQ P  S LLDSSF+GTPLAPP   XXXXXXXXXXXXXXXXXXXXXX P
Sbjct: 1   MASMASTLTTKLQTPSSSSLLDSSFHGTPLAPP---XXXXXXXXXXXXXXXXXXXXXXXP 60

Query: 61  YDLNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRI 120
           YDLN FKFNPIKESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNP IRI
Sbjct: 61  YDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRI 120

Query: 121 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIM 180
           AIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQEDYVVIKHAALFTSTIM
Sbjct: 121 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIM 180

Query: 181 SKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 240
           SKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS
Sbjct: 181 SKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 240

Query: 241 CGHDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEI 300
           CGHDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEI
Sbjct: 241 CGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEI 300

Query: 301 DGAPRMGPTFGAMMISGQKAAHLALKSLGEANGIEDGEVGERKVVEEPELLLAAGESPEV 360
           DGAPRMGPTFGAMMISGQKAAHLALKSLG AN IED         E  +L+LAAGESPEV
Sbjct: 301 DGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIED---------EAADLILAAGESPEV 350

The following BLAST results are available for this feature:
BLAST of Bhi11G000513 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G54770.11.3e-14674.52thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)[more]
BLAST of Bhi11G000513 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|O23787|THI4_CITSI9.7e-15277.41Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=... [more]
sp|F6H7K5|THI42_VITVI2.9e-14876.37Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2... [more]
sp|Q38709|THI4_ALNGL6.5e-14876.94Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=... [more]
sp|Q38814|THI4_ARATH2.3e-14574.52Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THI... [more]
sp|F6H9A9|THI41_VITVI2.6e-14484.26Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1... [more]
BLAST of Bhi11G000513 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3CJ81|A0A1S3CJ81_CUCME4.8e-17289.53Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10350160... [more]
tr|A0A0A0L546|A0A0A0L546_CUCSA8.2e-17288.71Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=... [more]
tr|A0A2I4EX88|A0A2I4EX88_9ROSI8.5e-15378.85Thiamine thiazole synthase, chloroplastic OS=Juglans regia OX=51240 GN=LOC108993... [more]
tr|A0A2P4JI82|A0A2P4JI82_QUESU1.1e-15278.95Thiamine thiazole synthase, chloroplastic OS=Quercus suber OX=58331 GN=THI1 PE=3... [more]
tr|A0A061F760|A0A061F760_THECC8.0e-15177.62Thiamine thiazole synthase, chloroplastic OS=Theobroma cacao OX=3641 GN=THI1 PE=... [more]
BLAST of Bhi11G000513 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008463440.17.3e-17289.53PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo][more]
XP_004152916.11.2e-17188.71PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis sativus] >KGN56149... [more]
XP_022943248.14.7e-16385.87thiamine thiazole synthase, chloroplastic [Cucurbita moschata][more]
XP_022985241.12.3e-16285.08thiamine thiazole synthase, chloroplastic-like [Cucurbita maxima][more]
XP_023524627.18.9e-16291.44thiamine thiazole synthase, chloroplastic [Cucurbita pepo subsp. pepo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0009228thiamine biosynthetic process
GO:0006950response to stress
Vocabulary: INTERPRO
TermDefinition
IPR027495Sti35
IPR002922Thi4_fam
IPR036188FAD/NAD-bd_sf
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006950 response to stress
biological_process GO:0009228 thiamine biosynthetic process
biological_process GO:0052837 thiazole biosynthetic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0018131 oxazole or thiazole biosynthetic process
biological_process GO:0006974 cellular response to DNA damage stimulus
cellular_component GO:0009579 thylakoid
cellular_component GO:0010319 stromule
cellular_component GO:0005739 mitochondrion
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0009507 chloroplast
cellular_component GO:0005575 cellular_component
cellular_component GO:0005829 cytosol
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016763 transferase activity, transferring pentosyl groups
molecular_function GO:0005506 iron ion binding
molecular_function GO:0042803 protein homodimerization activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi11M000513Bhi11M000513mRNA


InterPro
Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3DG3DSA:3.50.50.60coord: 91..326
e-value: 3.9E-113
score: 378.5
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILYSSF51905FAD/NAD(P)-binding domaincoord: 50..320
NoneNo IPR availablePFAMPF01946Thi4coord: 75..308
e-value: 5.0E-114
score: 379.3
NoneNo IPR availablePANTHERPTHR43422:SF3THIAMINE THIAZOLE SYNTHASEcoord: 52..335
NoneNo IPR availablePANTHERPTHR43422FAMILY NOT NAMEDcoord: 52..335
IPR002922Thiazole biosynthetic enzyme Thi4 familyTIGRFAMTIGR00292TIGR00292coord: 71..326
e-value: 3.0E-82
score: 273.6
IPR027495Thiamine thiazole synthaseHAMAPMF_03158THI4coord: 5..336
score: 68.039