Cp4.1LG01g08250 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG01g08250
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionThiamine thiazole synthase, chloroplastic
LocationCp4.1LG01 : 5053424 .. 5055018 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCATGGCTTCCACTCTCACCACCAAGCTCCAAACCCCCTCCTCCTCCTCCTTACTCGACTCCTCTTTTCATGGCACCCCTCTGGCCCCTCCTCCTCCTTCTCTCCGCCTCAAACCCACCCCTGCCTCTTTCTCCATCTCTGCCTCCTCCTCTCAGCAGCAGCCCTACGATTTGAACTCCTTCAAATTCAACCCCATCAAGGAGTCCATCGTCTCACGTGAGATGACCCGCCGTTACATGACCGACATGATCACTTACGCCGACACTGATGTCGTCATCGTCGGCGCTGGCTCTGCCGGTCTCTCTTGCGCTTATGAACTTAGCAAAAACCCTGATATTCGCATCGCCATCATCGAGCAGTCCGTCTCCCCCGGCGGCGGTGCATGGCTCGGTGGCCAGCTCTTCTCTGCTATGGTATGCTTTTAATTCCCTTATACGCCCCCTTTTCTTTCCTGTCTTCTAGCTTTGAATGCTCTGTTTTTTGTAAGAAAATACCCACAATTAGATCATACTAATTCAGTCATAAATTGGCTTAAGCCCAAGAATTTAAATACTCAATCAGTACAAGATCTGATTCTAAAATCATTAATCTGATGGGTTTGGGCAAATTTAATGTTTGATTCATATTCTTTGATAGGTGGTACGGAAGCCAGCCCACTGCTTCTTGGACGAGGTTGGTGTGGAGTACGACGAGCAAGAGGACTACGTTGTAATTAAGCACGCGGCTCTGTTCACCTCAACGATCATGAGCAAGCTGCTGGCACGGCCCAATGTCAAGCTGTTCAACGCCGTGGCGGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGTGTCGTCACCAACTGGGCTTTAGTGTCAATGAACCATGACACACAGTCCTGCATGGATCCTAATGTCATGGAGGCAAAGGTGGTGGTCAGCTCTTGTGGCCACGACGGGCCCTTCGGAGCCACCGGAGTGAAGCGACTTAAGAGCATTGGCATGATCGATTCCGTCCCGGGAATGAAGGCACTGGACATGAACACGGCGGAGGATGCCATTGTTAGGCTCACCCGAGAGGTAGTGCCTGGCATGATCGTAACGGGTATGGAGGTGGCGGAGATCGACGGAGCTCCAAGAATGGTAAAGAACTAAATTTGAATTAATGGTTTAACTAAAAAGCTTAGACCTTGTTTAACAATCAGAGGTGGTGTTGGATGCAGGGGCCAACGTTCGGGGCAATGATGATATCAGGGCAGAAGGCGGCGCACCTGGCGTTGAAGTCATTGGGGCTGGCAAATTGCATTGAGGATGAAGCAGCTGATCTGATACTGGCGGCGGGAGAGTCGCCGGAGGTTGCAGATGGTTGAAGGGTGAGTTTTCATGGATGGAAGGCGGCGGTTGTGTTTTGTGATTCATGGGGGCTCTGTTTCCGTGGCAAACAGATTGGGGTTTGGGGAAAAGCCAAATTTTAAGTTAAATTAAATAAAATTGTATCAAAATGTTAATTGCCTTTTTCTTCTCCCTAATTTTAAGTTAAATTATTATAAAATGAGATTTAAATTAATAATTCTGTAGTTAATAAATTATTGAGTGTTTAAATTTA

mRNA sequence

ATGGCTTCCATGGCTTCCACTCTCACCACCAAGCTCCAAACCCCCTCCTCCTCCTCCTTACTCGACTCCTCTTTTCATGGCACCCCTCTGGCCCCTCCTCCTCCTTCTCTCCGCCTCAAACCCACCCCTGCCTCTTTCTCCATCTCTGCCTCCTCCTCTCAGCAGCAGCCCTACGATTTGAACTCCTTCAAATTCAACCCCATCAAGGAGTCCATCGTCTCACGTGAGATGACCCGCCGTTACATGACCGACATGATCACTTACGCCGACACTGATGTCGTCATCGTCGGCGCTGGCTCTGCCGGTCTCTCTTGCGCTTATGAACTTAGCAAAAACCCTGATATTCGCATCGCCATCATCGAGCAGTCCGTCTCCCCCGGCGGCGGTGCATGGCTCGGTGGCCAGCTCTTCTCTGCTATGGTGGTACGGAAGCCAGCCCACTGCTTCTTGGACGAGGTTGGTGTGGAGTACGACGAGCAAGAGGACTACGTTGTAATTAAGCACGCGGCTCTGTTCACCTCAACGATCATGAGCAAGCTGCTGGCACGGCCCAATGTCAAGCTGTTCAACGCCGTGGCGGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGTGTCGTCACCAACTGGGCTTTAGTGTCAATGAACCATGACACACAGTCCTGCATGGATCCTAATGTCATGGAGGCAAAGGTGGTGGTCAGCTCTTGTGGCCACGACGGGCCCTTCGGAGCCACCGGAGTGAAGCGACTTAAGAGCATTGGCATGATCGATTCCGTCCCGGGAATGAAGGCACTGGACATGAACACGGCGGAGGATGCCATTGTTAGGCTCACCCGAGAGGTAGTGCCTGGCATGATCGTAACGGGTATGGAGGTGGCGGAGATCGACGGAGCTCCAAGAATGGGGCCAACGTTCGGGGCAATGATGATATCAGGGCAGAAGGCGGCGCACCTGGCGTTGAAGTCATTGGGGCTGGCAAATTGCATTGAGGATGAAGCAGCTGATCTGATACTGGCGGCGGGAGAGTCGCCGGAGGTTGCAGATGGTTGAAGGGTGAGTTTTCATGGATGGAAGGCGGCGGTTGTGTTTTGTGATTCATGGGGGCTCTGTTTCCGTGGCAAACAGATTGGGGTTTGGGGAAAAGCCAAATTTTAAGTTAAATTAAATAAAATTGTATCAAAATGTTAATTGCCTTTTTCTTCTCCCTAATTTTAAGTTAAATTATTATAAAATGAGATTTAAATTAATAATTCTGTAGTTAATAAATTATTGAGTGTTTAAATTTA

Coding sequence (CDS)

ATGGCTTCCATGGCTTCCACTCTCACCACCAAGCTCCAAACCCCCTCCTCCTCCTCCTTACTCGACTCCTCTTTTCATGGCACCCCTCTGGCCCCTCCTCCTCCTTCTCTCCGCCTCAAACCCACCCCTGCCTCTTTCTCCATCTCTGCCTCCTCCTCTCAGCAGCAGCCCTACGATTTGAACTCCTTCAAATTCAACCCCATCAAGGAGTCCATCGTCTCACGTGAGATGACCCGCCGTTACATGACCGACATGATCACTTACGCCGACACTGATGTCGTCATCGTCGGCGCTGGCTCTGCCGGTCTCTCTTGCGCTTATGAACTTAGCAAAAACCCTGATATTCGCATCGCCATCATCGAGCAGTCCGTCTCCCCCGGCGGCGGTGCATGGCTCGGTGGCCAGCTCTTCTCTGCTATGGTGGTACGGAAGCCAGCCCACTGCTTCTTGGACGAGGTTGGTGTGGAGTACGACGAGCAAGAGGACTACGTTGTAATTAAGCACGCGGCTCTGTTCACCTCAACGATCATGAGCAAGCTGCTGGCACGGCCCAATGTCAAGCTGTTCAACGCCGTGGCGGCAGAGGATTTGATCGTCAAGGGCGGCAGAGTTGGCGGTGTCGTCACCAACTGGGCTTTAGTGTCAATGAACCATGACACACAGTCCTGCATGGATCCTAATGTCATGGAGGCAAAGGTGGTGGTCAGCTCTTGTGGCCACGACGGGCCCTTCGGAGCCACCGGAGTGAAGCGACTTAAGAGCATTGGCATGATCGATTCCGTCCCGGGAATGAAGGCACTGGACATGAACACGGCGGAGGATGCCATTGTTAGGCTCACCCGAGAGGTAGTGCCTGGCATGATCGTAACGGGTATGGAGGTGGCGGAGATCGACGGAGCTCCAAGAATGGGGCCAACGTTCGGGGCAATGATGATATCAGGGCAGAAGGCGGCGCACCTGGCGTTGAAGTCATTGGGGCTGGCAAATTGCATTGAGGATGAAGCAGCTGATCTGATACTGGCGGCGGGAGAGTCGCCGGAGGTTGCAGATGGTTGA

Protein sequence

MASMASTLTTKLQTPSSSSLLDSSFHGTPLAPPPPSLRLKPTPASFSISASSSQQQPYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAADLILAAGESPEVADG
Homology
[Close]
BLAST of Cp4.1LG01g08250 vs. Swiss-Prot
Match: THI4_CITSI (Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis GN=THI1 PE=2 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 8.1e-156
Identity = 292/359 (81.34%), Postives = 318/359 (88.58%), Query Frame = 1 

Query: 1   MASMASTLTTKLQTPSSSSLLDSSFHGTPLAPPPPSLRLKP----TPASFSISASSSQQQ 60
           MA+MAST      + +++ L DSSFHG P++  P  LRL+P     P + SISAS+S   
Sbjct: 1   MAAMASTAFAPSVSSTTNKLFDSSFHGAPMS--PSLLRLQPIKSSRPNNLSISASAS--P 60

Query: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120
           PYDLN+FKF+PIKESIVSREMTRRYMTDMITYADTDVV+VGAGSAGLSCAYELSKNP+I+
Sbjct: 61  PYDLNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQ 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G++YDEQ++YVVIKHAALFTSTI
Sbjct: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRLKSIGMI+ VPGMKALDMN+AEDAIVRLTREVVPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAA-----DLILAAGESPEVAD 351
           IDGAPRMGPTFGAMMISGQKAAHLALKSLG  N ++         +LILAA +S E AD
Sbjct: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDGTYVGGVHPELILAAADSAETAD 355

[Close]
BLAST of Cp4.1LG01g08250 vs. Swiss-Prot
Match: THI4_ALNGL (Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa GN=THI1 PE=2 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 1.3e-150
Identity = 282/354 (79.66%), Postives = 309/354 (87.29%), Query Frame = 1 

Query: 4   MASTLTTKLQTPSSSSLLDSSFHGTPLAPPPPSLRLKPTPASF--SISASSSQQQPYDLN 63
           MASTLT+K Q  +      SSF+GTPLAP   S+R++PT A    SIS S +   PYDL 
Sbjct: 1   MASTLTSKPQRLALFENSASSFYGTPLAPS--SIRVQPTKAGAKPSISMSGAPSPPYDLK 60

Query: 64  SFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAIIE 123
           +F F+PIKESIVSREMTRRYM DMITYADTDVV+VGAGS+GL C YELSKNP +++AIIE
Sbjct: 61  AFTFDPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSSGLVC-YELSKNPSVQVAIIE 120

Query: 124 QSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLL 183
           QSVSPGGGAWLGGQLFS MVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTIMSKLL
Sbjct: 121 QSVSPGGGAWLGGQLFSGMVVRKPAHLFLDELGIEYDEQDNYVVIKHAALFTSTIMSKLL 180

Query: 184 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 243
           ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD
Sbjct: 181 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240

Query: 244 GPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAP 303
           GPFGATGVK L+SIGMID+VPGMKALDMN AEDAIVRLTRE+VPGMIVTGMEVAEIDGAP
Sbjct: 241 GPFGATGVKSLRSIGMIDTVPGMKALDMNVAEDAIVRLTREIVPGMIVTGMEVAEIDGAP 300

Query: 304 RMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAA-----DLILAAGESPEVAD 351
           RMGPTFGAMMISGQKAAHLALK+LGL N ++         +LILAA +S E+AD
Sbjct: 301 RMGPTFGAMMISGQKAAHLALKALGLPNALDGSYVGGIHPELILAAADSAEIAD 351

[Close]
BLAST of Cp4.1LG01g08250 vs. Swiss-Prot
Match: THI42_VITVI (Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera GN=THI1-2 PE=3 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 8.7e-150
Identity = 286/359 (79.67%), Postives = 306/359 (85.24%), Query Frame = 1 

Query: 1   MASMASTLTTKLQTPSSSSLLD-SSFHGTPLAPPPPSLRLKPTPASF-SISASSSQQQPY 60
           MASMA+TLT+    P  +   + SSFHG+P+     S R+ P   S  S + S S   PY
Sbjct: 1   MASMATTLTSLSSNPKPAFFDNKSSFHGSPI-----SYRVLPIKVSHQSPTISMSSVSPY 60

Query: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 120
           DL SFKF PIKESIV+REMTRRYM DMITYADTDVVIVGAGSAGLSCAYELSKNP IR+A
Sbjct: 61  DLQSFKFEPIKESIVAREMTRRYMMDMITYADTDVVIVGAGSAGLSCAYELSKNPSIRVA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHHFLDELGIEYDEQDNYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVK  RV GVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKEERVAGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTRE+VPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCI-------EDEAADLILAAGESPEVAD 351
           GAPRMGPTFGAMMISGQKAAHLAL++LG  N I       E    +LILAA E+ E+ D
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALRALGQPNAIDGNYTEAETMQPELILAAAETGEIVD 354

[Close]
BLAST of Cp4.1LG01g08250 vs. Swiss-Prot
Match: THI41_VITVI (Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera GN=THI1-1 PE=3 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 4.8e-148
Identity = 273/354 (77.12%), Postives = 310/354 (87.57%), Query Frame = 1 

Query: 6   STLTTKLQTPSSSSLLD---SSFHGTPLAPPPPSLRLKPTPASFSISASSSQQQPYDLNS 65
           +TLT+ + +   +S+ D   SSFHG P+A       +K TP + +++A++    PYDL S
Sbjct: 2   ATLTSSICSKPKASVFDPHKSSFHGVPIATQARLSPVKSTPVNLAVTAAA---MPYDLRS 61

Query: 66  FKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAIIEQ 125
           FKF PIKESIVSREMTRRYM DMITYADTDVV+VGAGSAGLSCAYELSKNP +++AIIEQ
Sbjct: 62  FKFEPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPSVQVAIIEQ 121

Query: 126 SVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLA 185
           SVSPGGGAWLGGQLFS+MVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTIMSKLLA
Sbjct: 122 SVSPGGGAWLGGQLFSSMVVRKPAHRFLDELGLEYDEQDNYVVIKHAALFTSTIMSKLLA 181

Query: 186 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 245
           RPNVKLFNAVAAEDLI+K G+VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 182 RPNVKLFNAVAAEDLIIKEGKVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 241

Query: 246 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPR 305
           PFGATGVKRL+S+GMIDSVPGMKALDMNTAED IVRLTREVVPGMIVTGMEVAEIDG+PR
Sbjct: 242 PFGATGVKRLRSVGMIDSVPGMKALDMNTAEDEIVRLTREVVPGMIVTGMEVAEIDGSPR 301

Query: 306 MGPTFGAMMISGQKAAHLALKSLGLANCIE-----DEAADLIL-AAGESPEVAD 351
           MGPTFGAMMISGQKAAHLALKSLGL N ++     +   +L+L AA ++ E+A+
Sbjct: 302 MGPTFGAMMISGQKAAHLALKSLGLPNALDGTYIGNLHPELVLAAAADAAEIAE 352

[Close]
BLAST of Cp4.1LG01g08250 vs. Swiss-Prot
Match: THI4_ARATH (Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana GN=THI1 PE=1 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 8.2e-148
Identity = 275/355 (77.46%), Postives = 309/355 (87.04%), Query Frame = 1 

Query: 1   MASMASTLTTKLQTPSSSSLLDSSFHGTPLAPPPPSLRLKPTPASFSISASSSQQQPYDL 60
           MA++ASTL+  L +     L DSSFHG+ ++  P S+ LKP   SFS+ A+++    YDL
Sbjct: 1   MAAIASTLS--LSSTKPQRLFDSSFHGSAISAAPISIGLKPR--SFSVRATTAG---YDL 60

Query: 61  NSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAII 120
           N+F F+PIKESIVSREMTRRYMTDMITYA+TDVV+VGAGSAGLS AYE+SKNP++++AII
Sbjct: 61  NAFTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAII 120

Query: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKL 180
           EQSVSPGGGAWLGGQLFSAM+VRKPAH FLDE+GV YDEQ+ YVV+KHAALFTSTIMSKL
Sbjct: 121 EQSVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSKL 180

Query: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240
           LARPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSCGH
Sbjct: 181 LARPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCGH 240

Query: 241 DGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGA 300
           DGPFGATGVKRLKSIGMID VPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGA
Sbjct: 241 DGPFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGA 300

Query: 301 PRMGPTFGAMMISGQKAAHLALKSLGLANCIE-----DEAADLILAAGESPEVAD 351
           PRMGPTFGAMMISGQKA  LALK+LGL N I+     + + +L+LAA +S E  D
Sbjct: 301 PRMGPTFGAMMISGQKAGQLALKALGLPNAIDGTLVGNLSPELVLAAADSAETVD 348

[Close]
BLAST of Cp4.1LG01g08250 vs. TrEMBL
Match: A0A0A0L546_CUCSA (Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus GN=THI1 PE=3 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 1.0e-165
Identity = 321/362 (88.67%), Postives = 329/362 (90.88%), Query Frame = 1 

Query: 1   MASMASTLTTKLQTPSSSSLLDSSFHGTPLAPPPPSLRLKPTPA-SFSISASSSQQQPYD 60
           MAS+ASTLTTKLQ PS   L DSSFHGTPLA P  SLRLK T A S +ISAS+SQ  PYD
Sbjct: 1   MASIASTLTTKLQKPSL--LHDSSFHGTPLASPS-SLRLKSTAATSLTISASASQP-PYD 60

Query: 61  LNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAI 120
           LN FKFNPI+ESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNP+IRIAI
Sbjct: 61  LNQFKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEA-----------ADLILAAGESPEV 351
           APRMGPTFGAMMISGQKAAHLALKSLG AN I DE             +L+LAAGESPEV
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEV 358

[Close]
BLAST of Cp4.1LG01g08250 vs. TrEMBL
Match: W9S2E9_9ROSA (Thiamine thiazole synthase, chloroplastic OS=Morus notabilis GN=THI1 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 6.1e-158
Identity = 292/355 (82.25%), Postives = 320/355 (90.14%), Query Frame = 1 

Query: 4   MASTLTTKLQTPSSSSLLDSS--FHGTPLAPPPPSLRLKPTPASFSISASSSQQQPYDLN 63
           MASTL+T     + +SL +SS  FHGTPLAPPPP LRL+P  A FS+S S++   PYDL 
Sbjct: 1   MASTLSTS-SFAAKTSLFESSSSFHGTPLAPPPPCLRLQPAKAGFSVSMSAAA--PYDLK 60

Query: 64  SFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAIIE 123
           +F+F PIKESIVSREMTRRYM DMITYADTDVV+VGAGSAGLSCAYELSKNPD+++AIIE
Sbjct: 61  NFRFEPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPDVQVAIIE 120

Query: 124 QSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLL 183
           QSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTIMSKLL
Sbjct: 121 QSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIEYDEQDNYVVIKHAALFTSTIMSKLL 180

Query: 184 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 243
           ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCGHD
Sbjct: 181 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHD 240

Query: 244 GPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAP 303
           GPFGATGVKRL+SIGMIDSVPGMKALDMN AEDAIVRLTRE+VPGMIVTGMEVAEIDG+P
Sbjct: 241 GPFGATGVKRLRSIGMIDSVPGMKALDMNAAEDAIVRLTREIVPGMIVTGMEVAEIDGSP 300

Query: 304 RMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAA------DLILAAGESPEVAD 351
           RMGPTFGAMMISGQKAAHLALKSLGL N ++  +       +LILAA E+ E+AD
Sbjct: 301 RMGPTFGAMMISGQKAAHLALKSLGLPNALDGSSVGSNIHPELILAAAENAEIAD 352

[Close]
BLAST of Cp4.1LG01g08250 vs. TrEMBL
Match: A0A067ECZ8_CITSI (Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis GN=THI1 PE=3 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 1.8e-154
Identity = 293/360 (81.39%), Postives = 319/360 (88.61%), Query Frame = 1 

Query: 1   MASMASTLTTKLQTPSSSSLLDSSFHGTPLAPPPPSLRLKP----TPASFSISASSSQQQ 60
           MA+MAST      + +++ L DSSFHG P++  P  LRL+P     P + SISAS+S   
Sbjct: 1   MAAMASTAFAPSVSSTTNKLFDSSFHGAPMS--PSLLRLQPIKSSRPNNLSISASAS--P 60

Query: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120
           PYDLN+FKF+PIKESIVSREMTRRYMTDMITYADTDVV+VGAGSAGLSCAYELSKNP+I+
Sbjct: 61  PYDLNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQ 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G++YDEQ++YVVIKHAALFTSTI
Sbjct: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRLKSIGMI+ VPGMKALDMN+AEDAIVRLTREVVPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAA-----DLILAAGESPEVADG 352
           IDGAPRMGPTFGAMMISGQKAAHLALKSLG  N ++         +LILAA +S E ADG
Sbjct: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDGTYVGGVHPELILAAADSAETADG 356

[Close]
BLAST of Cp4.1LG01g08250 vs. TrEMBL
Match: D6N3H1_GOSHI (Thiamine thiazole synthase, chloroplastic OS=Gossypium hirsutum GN=THI1 PE=2 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 4.1e-154
Identity = 285/354 (80.51%), Postives = 314/354 (88.70%), Query Frame = 1 

Query: 2   ASMASTLTTKLQTPSSSSLLDSSFHGTPLAPPPPSLRLKPTPASFSISASSSQQQPYDLN 61
           +S+A+TLT+  +   ++SL +SSFHG P+ P     + K +P + SIS S++   PYDLN
Sbjct: 3   SSIATTLTSSSKLCRNTSLFESSFHGVPIKPLSFHFKTKSSPCNASISMSAASPPPYDLN 62

Query: 62  SFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAIIE 121
           +F+F+PIKESIVSREMTRRYM DMITYADTDVV+VGAGSAGLSCAYELSKNP ++IAI+E
Sbjct: 63  NFRFDPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPSVQIAIVE 122

Query: 122 QSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLL 181
           QSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ +EYDEQ+DYVVIKHAALFTSTIMSKLL
Sbjct: 123 QSVSPGGGAWLGGQLFSAMVVRKPAHRFLDELAIEYDEQDDYVVIKHAALFTSTIMSKLL 182

Query: 182 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 241
           ARPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD
Sbjct: 183 ARPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 242

Query: 242 GPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAP 301
           GPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTRE+VPGMIVTGMEVAEIDG+P
Sbjct: 243 GPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEIDGSP 302

Query: 302 RMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAA-----DLILAAGESPEVAD 351
           RMGPTFGAMMISGQKAAHLALKSLGL N I+         +LILAA +S E AD
Sbjct: 303 RMGPTFGAMMISGQKAAHLALKSLGLPNAIDGTYVGSIHPELILAAADSAETAD 356

[Close]
BLAST of Cp4.1LG01g08250 vs. TrEMBL
Match: A0A061F760_THECC (Thiamine thiazole synthase, chloroplastic OS=Theobroma cacao GN=THI1 PE=3 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 9.1e-154
Identity = 284/355 (80.00%), Postives = 317/355 (89.30%), Query Frame = 1 

Query: 2   ASMASTLTTKLQTPSSSSLLDSSFHGTPLAPPPPSLRLKPTPASFSISASS-SQQQPYDL 61
           +S+A+TLT+  +   ++SL +SSFHG P+ P    L+ K +P + S+S S+ S   PYDL
Sbjct: 3   SSVATTLTSSTKLYRNTSLFESSFHGVPITPLSFHLKAKSSPRNASVSMSAASSPPPYDL 62

Query: 62  NSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAII 121
           N+F+F+PIKESIVSREMTRRYM DMIT+ADTDVV+VGAGSAGLSCAYELSKNP +++AI+
Sbjct: 63  NNFRFDPIKESIVSREMTRRYMMDMITHADTDVVVVGAGSAGLSCAYELSKNPSVQVAIV 122

Query: 122 EQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKL 181
           EQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQ+DYVVIKHAALFTSTIMSKL
Sbjct: 123 EQSVSPGGGAWLGGQLFSAMVVRKPAHRFLDELGIEYDEQDDYVVIKHAALFTSTIMSKL 182

Query: 182 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 241
           LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH
Sbjct: 183 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 242

Query: 242 DGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGA 301
           DGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTRE+VPGMIVTGMEVAEIDG+
Sbjct: 243 DGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEIDGS 302

Query: 302 PRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAA-----DLILAAGESPEVAD 351
           PRMGPTFGAMMISGQKAAHLAL+SLGL N ++         +LILAA +S E AD
Sbjct: 303 PRMGPTFGAMMISGQKAAHLALQSLGLPNALDGTYVGNIHPELILAAADSAETAD 357

[Close]
BLAST of Cp4.1LG01g08250 vs. TAIR10
Match: AT5G54770.1 (AT5G54770.1 thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4))

HSP 1 Score: 524.6 bits (1350), Expect = 4.6e-149
Identity = 275/355 (77.46%), Postives = 309/355 (87.04%), Query Frame = 1 

Query: 1   MASMASTLTTKLQTPSSSSLLDSSFHGTPLAPPPPSLRLKPTPASFSISASSSQQQPYDL 60
           MA++ASTL+  L +     L DSSFHG+ ++  P S+ LKP   SFS+ A+++    YDL
Sbjct: 1   MAAIASTLS--LSSTKPQRLFDSSFHGSAISAAPISIGLKPR--SFSVRATTAG---YDL 60

Query: 61  NSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAII 120
           N+F F+PIKESIVSREMTRRYMTDMITYA+TDVV+VGAGSAGLS AYE+SKNP++++AII
Sbjct: 61  NAFTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAII 120

Query: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKL 180
           EQSVSPGGGAWLGGQLFSAM+VRKPAH FLDE+GV YDEQ+ YVV+KHAALFTSTIMSKL
Sbjct: 121 EQSVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSKL 180

Query: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240
           LARPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSCGH
Sbjct: 181 LARPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCGH 240

Query: 241 DGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGA 300
           DGPFGATGVKRLKSIGMID VPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGA
Sbjct: 241 DGPFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGA 300

Query: 301 PRMGPTFGAMMISGQKAAHLALKSLGLANCIE-----DEAADLILAAGESPEVAD 351
           PRMGPTFGAMMISGQKA  LALK+LGL N I+     + + +L+LAA +S E  D
Sbjct: 301 PRMGPTFGAMMISGQKAGQLALKALGLPNAIDGTLVGNLSPELVLAAADSAETVD 348

[Close]
BLAST of Cp4.1LG01g08250 vs. NCBI nr
Match: gi|449470423|ref|XP_004152916.1| (PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis sativus])

HSP 1 Score: 590.9 bits (1522), Expect = 1.5e-165
Identity = 321/362 (88.67%), Postives = 329/362 (90.88%), Query Frame = 1 

Query: 1   MASMASTLTTKLQTPSSSSLLDSSFHGTPLAPPPPSLRLKPTPA-SFSISASSSQQQPYD 60
           MAS+ASTLTTKLQ PS   L DSSFHGTPLA P  SLRLK T A S +ISAS+SQ  PYD
Sbjct: 1   MASIASTLTTKLQKPSL--LHDSSFHGTPLASPS-SLRLKSTAATSLTISASASQP-PYD 60

Query: 61  LNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAI 120
           LN FKFNPI+ESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNP+IRIAI
Sbjct: 61  LNQFKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEA-----------ADLILAAGESPEV 351
           APRMGPTFGAMMISGQKAAHLALKSLG AN I DE             +L+LAAGESPEV
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEV 358

[Close]
BLAST of Cp4.1LG01g08250 vs. NCBI nr
Match: gi|659126941|ref|XP_008463440.1| (PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo])

HSP 1 Score: 589.3 bits (1518), Expect = 4.3e-165
Identity = 318/362 (87.85%), Postives = 328/362 (90.61%), Query Frame = 1 

Query: 1   MASMASTLTTKLQTPSSSSLLDSSFHGTPLAPPPPSLRLK-PTPASFSISASSSQQQPYD 60
           MAS+ASTLTTKLQ PS   L DSSFHGTPLA PP SLRLK P   S +ISAS+SQ  PYD
Sbjct: 1   MASIASTLTTKLQIPSL--LHDSSFHGTPLASPP-SLRLKSPAATSLTISASASQP-PYD 60

Query: 61  LNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAI 120
           LN FKFNPI+ESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNP+IRIAI
Sbjct: 61  LNQFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEA-----------ADLILAAGESPEV 351
           APRMGPTFGAMMISGQKAAHLALKSLG AN I  E             +L+LAAGESPEV
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEV 358

[Close]
BLAST of Cp4.1LG01g08250 vs. NCBI nr
Match: gi|703134159|ref|XP_010105570.1| (Thiamine thiazole synthase 2 [Morus notabilis])

HSP 1 Score: 565.1 bits (1455), Expect = 8.7e-158
Identity = 292/355 (82.25%), Postives = 320/355 (90.14%), Query Frame = 1 

Query: 4   MASTLTTKLQTPSSSSLLDSS--FHGTPLAPPPPSLRLKPTPASFSISASSSQQQPYDLN 63
           MASTL+T     + +SL +SS  FHGTPLAPPPP LRL+P  A FS+S S++   PYDL 
Sbjct: 1   MASTLSTS-SFAAKTSLFESSSSFHGTPLAPPPPCLRLQPAKAGFSVSMSAAA--PYDLK 60

Query: 64  SFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAIIE 123
           +F+F PIKESIVSREMTRRYM DMITYADTDVV+VGAGSAGLSCAYELSKNPD+++AIIE
Sbjct: 61  NFRFEPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPDVQVAIIE 120

Query: 124 QSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLL 183
           QSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQ++YVVIKHAALFTSTIMSKLL
Sbjct: 121 QSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIEYDEQDNYVVIKHAALFTSTIMSKLL 180

Query: 184 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 243
           ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCGHD
Sbjct: 181 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHD 240

Query: 244 GPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAP 303
           GPFGATGVKRL+SIGMIDSVPGMKALDMN AEDAIVRLTRE+VPGMIVTGMEVAEIDG+P
Sbjct: 241 GPFGATGVKRLRSIGMIDSVPGMKALDMNAAEDAIVRLTREIVPGMIVTGMEVAEIDGSP 300

Query: 304 RMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAA------DLILAAGESPEVAD 351
           RMGPTFGAMMISGQKAAHLALKSLGL N ++  +       +LILAA E+ E+AD
Sbjct: 301 RMGPTFGAMMISGQKAAHLALKSLGLPNALDGSSVGSNIHPELILAAAENAEIAD 352

[Close]
BLAST of Cp4.1LG01g08250 vs. NCBI nr
Match: gi|641833964|gb|KDO52968.1| (hypothetical protein CISIN_1g018414mg [Citrus sinensis])

HSP 1 Score: 553.5 bits (1425), Expect = 2.6e-154
Identity = 293/360 (81.39%), Postives = 319/360 (88.61%), Query Frame = 1 

Query: 1   MASMASTLTTKLQTPSSSSLLDSSFHGTPLAPPPPSLRLKP----TPASFSISASSSQQQ 60
           MA+MAST      + +++ L DSSFHG P++  P  LRL+P     P + SISAS+S   
Sbjct: 1   MAAMASTAFAPSVSSTTNKLFDSSFHGAPMS--PSLLRLQPIKSSRPNNLSISASAS--P 60

Query: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120
           PYDLN+FKF+PIKESIVSREMTRRYMTDMITYADTDVV+VGAGSAGLSCAYELSKNP+I+
Sbjct: 61  PYDLNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQ 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180
           IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G++YDEQ++YVVIKHAALFTSTI
Sbjct: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTI 180

Query: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240

Query: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRLKSIGMI+ VPGMKALDMN+AEDAIVRLTREVVPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAA-----DLILAAGESPEVADG 352
           IDGAPRMGPTFGAMMISGQKAAHLALKSLG  N ++         +LILAA +S E ADG
Sbjct: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDGTYVGGVHPELILAAADSAETADG 356

[Close]
BLAST of Cp4.1LG01g08250 vs. NCBI nr
Match: gi|295687239|gb|ADG27845.1| (thiazole biosynthetic enzyme [Gossypium hirsutum])

HSP 1 Score: 552.4 bits (1422), Expect = 5.8e-154
Identity = 285/354 (80.51%), Postives = 314/354 (88.70%), Query Frame = 1 

Query: 2   ASMASTLTTKLQTPSSSSLLDSSFHGTPLAPPPPSLRLKPTPASFSISASSSQQQPYDLN 61
           +S+A+TLT+  +   ++SL +SSFHG P+ P     + K +P + SIS S++   PYDLN
Sbjct: 3   SSIATTLTSSSKLCRNTSLFESSFHGVPIKPLSFHFKTKSSPCNASISMSAASPPPYDLN 62

Query: 62  SFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAIIE 121
           +F+F+PIKESIVSREMTRRYM DMITYADTDVV+VGAGSAGLSCAYELSKNP ++IAI+E
Sbjct: 63  NFRFDPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPSVQIAIVE 122

Query: 122 QSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLL 181
           QSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ +EYDEQ+DYVVIKHAALFTSTIMSKLL
Sbjct: 123 QSVSPGGGAWLGGQLFSAMVVRKPAHRFLDELAIEYDEQDDYVVIKHAALFTSTIMSKLL 182

Query: 182 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 241
           ARPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD
Sbjct: 183 ARPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 242

Query: 242 GPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAP 301
           GPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTRE+VPGMIVTGMEVAEIDG+P
Sbjct: 243 GPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEIDGSP 302

Query: 302 RMGPTFGAMMISGQKAAHLALKSLGLANCIEDEAA-----DLILAAGESPEVAD 351
           RMGPTFGAMMISGQKAAHLALKSLGL N I+         +LILAA +S E AD
Sbjct: 303 RMGPTFGAMMISGQKAAHLALKSLGLPNAIDGTYVGSIHPELILAAADSAETAD 356

The following BLAST results are available for this feature:
BLAST of Cp4.1LG01g08250 vs. Swiss-Prot
Match NameE-valueIdentityDescription
THI4_CITSI8.1e-15681.34Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis GN=THI1 PE=2 SV=1[more]
THI4_ALNGL1.3e-15079.66Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa GN=THI1 PE=2 SV=1[more]
THI42_VITVI8.7e-15079.67Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera GN=THI1-2 PE=3 SV=... [more]
THI41_VITVI4.8e-14877.12Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera GN=THI1-1 PE=3 SV=... [more]
THI4_ARATH8.2e-14877.46Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana GN=THI1 PE=1 S... [more]
BLAST of Cp4.1LG01g08250 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L546_CUCSA1.0e-16588.67Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus GN=THI1 PE=3 SV=1[more]
W9S2E9_9ROSA6.1e-15882.25Thiamine thiazole synthase, chloroplastic OS=Morus notabilis GN=THI1 PE=3 SV=1[more]
A0A067ECZ8_CITSI1.8e-15481.39Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis GN=THI1 PE=3 SV=1[more]
D6N3H1_GOSHI4.1e-15480.51Thiamine thiazole synthase, chloroplastic OS=Gossypium hirsutum GN=THI1 PE=2 SV=... [more]
A0A061F760_THECC9.1e-15480.00Thiamine thiazole synthase, chloroplastic OS=Theobroma cacao GN=THI1 PE=3 SV=1[more]
BLAST of Cp4.1LG01g08250 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G54770.14.6e-14977.46 thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)[more]
BLAST of Cp4.1LG01g08250 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449470423|ref|XP_004152916.1|1.5e-16588.67PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis sativus][more]
gi|659126941|ref|XP_008463440.1|4.3e-16587.85PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo][more]
gi|703134159|ref|XP_010105570.1|8.7e-15882.25Thiamine thiazole synthase 2 [Morus notabilis][more]
gi|641833964|gb|KDO52968.1|2.6e-15481.39hypothetical protein CISIN_1g018414mg [Citrus sinensis][more]
gi|295687239|gb|ADG27845.1|5.8e-15480.51thiazole biosynthetic enzyme [Gossypium hirsutum][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0009228thiamine biosynthetic process
GO:0006950response to stress
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: INTERPRO
TermDefinition
IPR027495Sti35
IPR023753FAD/NAD-binding_dom
IPR002922Thi4_fam
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006974 cellular response to DNA damage stimulus
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0009228 thiamine biosynthetic process
biological_process GO:0018131 oxazole or thiazole biosynthetic process
biological_process GO:0052837 thiazole biosynthetic process
biological_process GO:0006950 response to stress
cellular_component GO:0009507 chloroplast
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0005739 mitochondrion
cellular_component GO:0010319 stromule
cellular_component GO:0009579 thylakoid
cellular_component GO:0005829 cytosol
cellular_component GO:0005575 cellular_component
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0042803 protein homodimerization activity
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g08250.1Cp4.1LG01g08250.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002922Thiazole biosynthetic enzyme Thi4 familyTIGRFAMsTIGR00292TIGR00292coord: 70..325
score: 7.0
IPR023753FAD/NAD(P)-binding domainGENE3DG3DSA:3.50.50.60coord: 90..324
score: 2.8E
IPR023753FAD/NAD(P)-binding domainunknownSSF51905FAD/NAD(P)-binding domaincoord: 56..319
score: 2.44
IPR027495Thiamine thiazole synthaseHAMAPMF_03158THI4coord: 5..334
score: 68
NoneNo IPR availablePANTHERPTHR10617ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASEcoord: 66..332
score: 7.5E
NoneNo IPR availablePANTHERPTHR10617:SF115THIAMINE THIAZOLE SYNTHASEcoord: 66..332
score: 7.5E
NoneNo IPR availablePFAMPF01946Thi4coord: 74..307
score: 8.8E