MELO3C022770 (gene) Melon (DHL92) v3.5.1

BLAST of MELO3C022770 vs. Swiss-Prot
Match: 4CLL6_ARATH (4-coumarate--CoA ligase-like 6 OS=Arabidopsis thaliana GN=4CLL6 PE=2 SV=2)

HSP 1 Score: 607.8 bits (1566), Expect = 1.1e-172
Identity = 300/507 (59.17%), Postives = 393/507 (77.51%), Query Frame = 1

Query: 35  PNWYSPDTGICHSVHGYRKLPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYKELF 94
           P W+S  TGI  S      LP DP LD VS +FS   +H+G +ALIDSLTG SIS+ EL 
Sbjct: 22  PFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFS--HKHHGDTALIDSLTGFSISHTELQ 81

Query: 95  PMVKSMASGL-HNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKK 154
            MV+SMA+G+ H LG+ QGDVV L+LPNS+++P+I L  + LGA++TTM P SS  EIKK
Sbjct: 82  IMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSSSLGEIKK 141

Query: 155 RITDCNVRLAFTITQKIKNFEALGIKTIGVPENTNFDLMRPMGFSSFYELISSGSDLIKR 214
           ++++C+V LAFT T+ ++   +LG+  I V E+ +FD +R +    FY ++      + +
Sbjct: 142 QVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIR-IENPKFYSIMKESFGFVPK 201

Query: 215 PVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAAIP 274
           P+I+Q+D AAI++SSGTTG SKGV+L+HRN I+++ELFVRFEASQYEY  + NVYLAA+P
Sbjct: 202 PLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALP 261

Query: 275 MFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIALSRTAEK 334
           + HIYGLS+FVMGL+SLGS++VVM +FD  DVV  I+RFK+THFPVVPP+L+AL++ A+ 
Sbjct: 262 LCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLMALTKKAKG 321

Query: 335 IGVRRFRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGFNNKKTRN 394
           +    F+SLKQVS GAA  S+K I+DF+Q L HVD IQGYGMTESTA+GTRGFN++K   
Sbjct: 322 VCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRGFNSEKLSR 381

Query: 395 YLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDQENWL 454
           Y SVGLLAPN +AKVVDW +GS +PPG  GEL ++GPG MKGYLNNP+AT  +I +++WL
Sbjct: 382 YSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQMSIVEDSWL 441

Query: 455 HTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECG 514
            TGDI YFD DGYL++VDR+KE+IKYKGFQIAP DLEAV+++HP ++D AV AA +EECG
Sbjct: 442 RTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECG 501

Query: 515 EIPVAFVVKKPGSALSQKDVVDYVAQQ 541
           EIPVAFVV++  + LS++DV+ YVA Q
Sbjct: 502 EIPVAFVVRRQETTLSEEDVISYVASQ 525

BLAST of MELO3C022770 vs. Swiss-Prot
Match: 4CLL3_ORYSJ (4-coumarate--CoA ligase-like 3 OS=Oryza sativa subsp. japonica GN=4CLL3 PE=3 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 4.1e-130
Identity = 260/520 (50.00%), Postives = 336/520 (64.62%), Query Frame = 1

Query: 35  PNWYSPDTGICHSVHGYRKLPSDPFLDVVSFIFS-VQFQHNGHSALIDSLTGNSISYKEL 94
           P +YS  TGI  S+H    LP+DP L +V+ +F+ +     G   L+D+ T +++S  +L
Sbjct: 25  PPFYSAATGIYSSIHPPVALPTDPSLTLVAHLFARLPLADPGAPTLVDAATASAVSRADL 84

Query: 95  FPMVKSMASGLHNL-GISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIK 154
             +V S+A+GL    G+ +G VVLL+LPNS+ +P+  L  L  GAV TTM P SS +EI 
Sbjct: 85  RRLVASLAAGLRRRHGVRKGSVVLLLLPNSVAFPVSFLAVLAAGAVATTMNPSSSPAEIA 144

Query: 155 KRITDCNVRLAFTITQKIKNFEALGIKTIGVPENTNFDLMRPMG------FSSFYELISS 214
            +       L             L    + VPE  +       G      F++F  ++  
Sbjct: 145 AQARATGACLVLASRDGAARLPPLAAPVVLVPEILDHSAAADDGDDDQRVFAAFRAMLDG 204

Query: 215 GSD---LIKRPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLP 274
           G         PV+ Q+D  AIL+SSGT+G SKGV+L+HRN I+  ELFVRFEASQY    
Sbjct: 205 GGGDGTETAVPVVGQDDAVAILYSSGTSGRSKGVVLTHRNLIAMTELFVRFEASQYHARG 264

Query: 275 TK-NVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPP 334
            + NVY+AA+PM H+YGLS+F +GL+S+G++VVVM +FD  D V AI R+KVTH P+VPP
Sbjct: 265 ARENVYMAALPMSHVYGLSLFAVGLLSIGATVVVMRRFDAGDAVAAIGRYKVTHMPLVPP 324

Query: 335 ILIALSRTAEKIGV--RRFRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTA 394
           I+ A+ R A   GV   +  SL QVSCGAA  +   I +F+QA  HVDFIQGYGMTESTA
Sbjct: 325 IMAAMVRAAAAGGVPPSQVASLVQVSCGAAPITAALIHEFLQAFPHVDFIQGYGMTESTA 384

Query: 395 IGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNP 454
           +GTRGFN  K + Y SVGLLAPN  AK+V   + S +PPG +GEL L GPG MKGYL++ 
Sbjct: 385 VGTRGFNTSKHKKYTSVGLLAPNMHAKIVHLESSSCLPPGFSGELWLHGPGIMKGYLSDD 444

Query: 455 EATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVL 514
           +   +  D   WL TGDI YFD DGYLY+V RLK+ IKYKGFQIAP DLE V+I HPE+L
Sbjct: 445 DDACTRKD--GWLRTGDIAYFDLDGYLYIVGRLKDTIKYKGFQIAPGDLEEVLIHHPEIL 504

Query: 515 DTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 541
           D AV +A+DEE GEIPVAFVV++ GS LS K V++YVA+Q
Sbjct: 505 DVAVTSAEDEEAGEIPVAFVVRRSGSNLSCKQVMEYVAKQ 542

BLAST of MELO3C022770 vs. Swiss-Prot
Match: 4CLL2_ORYSJ (4-coumarate--CoA ligase-like 2 OS=Oryza sativa subsp. japonica GN=4CLL2 PE=3 SV=3)

HSP 1 Score: 454.9 bits (1169), Expect = 1.2e-126
Identity = 255/514 (49.61%), Postives = 333/514 (64.79%), Query Frame = 1

Query: 36  NWYSPDTGICHSVHGYRKLPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFP 95
           ++YS  TG+  S+H    LPSDP L +V  +FS    H+ HS L+D+ T  ++S  +   
Sbjct: 14  SFYSAATGLYSSLHPPLPLPSDPSLSLVPHLFSHLPLHH-HSLLVDAPTAATLSCADFRR 73

Query: 96  MVKSMASGLHN-LGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKKR 155
           +V S+A+GL   L I++G +VLL+LPNS+ +P+  L  L  GAV TTM P S+ +EI  R
Sbjct: 74  LVSSLAAGLRRRLHIARGSLVLLLLPNSLTFPVAFLAVLATGAVATTMNPSSAPAEIAAR 133

Query: 156 ITDCNVRLAFTITQKIKNFEALGIKTIGVPENTNFDLMRPMGFSSFYELISSGSDL-IKR 215
           + D    L    T        L    + VP+              F+ L+ +  +  ++ 
Sbjct: 134 LRDTAPSLVLASTHNAAKLPPLAAPLVLVPDTFQQQHDDDQFDFFFHALLETDPETPVEM 193

Query: 216 PV-IRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAAI 275
            V + Q+D AA+L+SSGT+G SKGV+++HRN I+ +ELFVRFEASQY      NVYLAA+
Sbjct: 194 GVGVGQDDAAAVLYSSGTSGRSKGVVVTHRNLIAMVELFVRFEASQYTRPARDNVYLAAL 253

Query: 276 PMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIALSRTAE 335
           PMFH+YGLS+F +GL+SLG +VVVM +F+V D VKAI ++KVTH P+VPPI+ AL R   
Sbjct: 254 PMFHVYGLSLFAVGLLSLGCTVVVMRRFNVDDAVKAIRKYKVTHLPLVPPIMSALLRANP 313

Query: 336 KIGVRRFRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGFNNKKTR 395
            +      SL QVS GAA  +   I  F+ A  HVDFIQGYGMTESTA+GTRGFN  K +
Sbjct: 314 PL---ELDSLLQVSSGAAPLNHTLIHHFLHAFPHVDFIQGYGMTESTAVGTRGFNTCKHK 373

Query: 396 NYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMK------GYLNNPEATMST 455
            Y SVGLLAPN  AK+V   +GS +PPG  GEL L GP  MK      GYLN+ +   + 
Sbjct: 374 KYASVGLLAPNMHAKIVHLESGSCLPPGSYGELWLHGPAIMKEFCFVTGYLNDDDDAFTR 433

Query: 456 IDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAA 515
             ++ WL TGDI YFD DGYL++V RLK+ IKYKGFQIAP DLEAV+I HPE++D AV +
Sbjct: 434 --KDGWLRTGDIAYFDSDGYLFIVGRLKDTIKYKGFQIAPADLEAVLIRHPEIIDVAVTS 493

Query: 516 AKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 541
            +DEE GEIPVAFVV+K GS LS   V++YVA+Q
Sbjct: 494 DEDEEAGEIPVAFVVRKSGSTLSCTHVMEYVAKQ 521

BLAST of MELO3C022770 vs. Swiss-Prot
Match: 4CLL5_ARATH (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 397.9 bits (1021), Expect = 1.8e-109
Identity = 212/491 (43.18%), Postives = 320/491 (65.17%), Query Frame = 1

Query: 54  LPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGD 113
           LP +P LDV +FI S    H G  A ID+ TG ++++ EL+  V+S+A  L  +GI +G 
Sbjct: 26  LPPNPSLDVTTFISSQA--HRGRIAFIDASTGQNLTFTELWRAVESVADCLSEIGIRKGH 85

Query: 114 VVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNF 173
           VVLL+ PNSI +P++ L  + LGA+ITT  P ++S+EI K+I D N  LAFT +Q +   
Sbjct: 86  VVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKI 145

Query: 174 EALGIKT-IGVPENTNFDLMRPMG-FSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTT 233
            A   K  I + +    D +  +       +   SG+ + +R  + Q+DTA +L+SSGTT
Sbjct: 146 SAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKEPSGNRVKER--VDQDDTATLLYSSGTT 205

Query: 234 GVSKGVMLSHRNFISTIELFV-RFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSL 293
           G+SKGV+ SHRN I+ ++  V RF +   E       ++  +PMFHIYGL+ F  GL++ 
Sbjct: 206 GMSKGVISSHRNLIAMVQTIVNRFGSDDGEQR-----FICTVPMFHIYGLAAFATGLLAY 265

Query: 294 GSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRR-FRSLKQVSCGA 353
           GS+++V+SKF++ +++ AI +++ T  P+VPPIL+A+   A++I  +    S+  V CG 
Sbjct: 266 GSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSSMHTVLCGG 325

Query: 354 AASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVV 413
           A  SK+  + F +    V  +QGYG+TEST IG      +++R Y + G L+ + E ++V
Sbjct: 326 APLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRIV 385

Query: 414 DWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYV 473
           D VTG  + P +TGEL L+GP  MKGY +N EAT ST+D E WL TGD+ Y D DG+++V
Sbjct: 386 DPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFV 445

Query: 474 VDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALS 533
           VDRLKE+IKYKG+Q+AP +LEA+++ HPE+ D AV    D+E G+ P+A+VV+K GS+LS
Sbjct: 446 VDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLS 505

Query: 534 QKDVVDYVAQQ 541
           +K ++++VA+Q
Sbjct: 506 EKTIMEFVAKQ 507

BLAST of MELO3C022770 vs. Swiss-Prot
Match: 4CLL4_ORYSJ (4-coumarate--CoA ligase-like 4 OS=Oryza sativa subsp. japonica GN=4CLL4 PE=2 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 2.6e-105
Identity = 207/511 (40.51%), Postives = 323/511 (63.21%), Query Frame = 1

Query: 42  TGICHSVHGYRK------LPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFP 101
           +G C +   +R       LP+DP +DVVSF+ S +  H+G  AL+D+ TG  I++ EL+ 
Sbjct: 13  SGYCAATRTFRSRRADVPLPADPEVDVVSFLASRR--HSGVVALVDAATGRRITFTELWR 72

Query: 102 MVKSMASGL--HNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKK 161
            V   AS L  H + + +G V L++ PNS+ +P+  L A+ LGAV+TT  P ++ +EI K
Sbjct: 73  AVAGAASALAAHPVSLRKGHVALILSPNSVHFPVAALAAMSLGAVLTTANPLNTPAEIAK 132

Query: 162 RITDCNVRLAFTITQKIKNFEALGIKTIGVPENTNF--DLMRPMGFSSFYELISSGSDLI 221
           ++ D    LAFT  + +          + + E+     D   P   ++  E+ ++  D  
Sbjct: 133 QVADARPVLAFTTRELLPKLPRAHDLRVVLLESARLPGDSSDPRIVATIEEISATTPDPA 192

Query: 222 KRPV-IRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFV-RFEASQYEYLPTKNVYL 281
           +R   + Q+D A +L+SSGTTG SKGV+ +HR+ IS +++ + RF     +   T   +L
Sbjct: 193 RRKDRVTQDDPATLLYSSGTTGPSKGVVATHRSLISMVQIIMTRFRLEGSDKTET---FL 252

Query: 282 AAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIALSR 341
             +PMFH+YGL  F  GL+  G++VVV+SK+++ +++++I+ + VT+ P+VPPIL+A+  
Sbjct: 253 CTVPMFHVYGLVAFATGLLGCGATVVVLSKYELPEMLRSINAYGVTYLPLVPPILVAMVA 312

Query: 342 TAEKIGVRRFRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGFNNK 401
             + + + + R   +V  G A   K+ I+ F +    V+ +QGYG+TESTAIG    + +
Sbjct: 313 HPKPLPLGQMR---KVLSGGAPLGKELIEGFREKYPQVEILQGYGLTESTAIGASTDSAE 372

Query: 402 KTRNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDQ 461
           ++R Y + GLL+PNTEAK+VD  +G ++P  +TGEL +RGP  MKGY  N EAT ST+  
Sbjct: 373 ESRRYGTAGLLSPNTEAKIVDPDSGEALPVNRTGELWIRGPYVMKGYFKNAEATQSTLTP 432

Query: 462 ENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAAAKD 521
           + WL TGD+ Y D DGYL+VVDRLKE+IKYKG+Q+ P +LEA+++ HPEV D AV    D
Sbjct: 433 DGWLKTGDLCYIDEDGYLFVVDRLKELIKYKGYQVPPAELEALLLTHPEVTDVAVIPFPD 492

Query: 522 EECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 541
            E G+ P+A++V+K GS LS+++V+++VA+Q
Sbjct: 493 REVGQFPMAYIVRKKGSNLSEREVMEFVAKQ 515

BLAST of MELO3C022770 vs. TrEMBL
Match: A0A0A0L8X1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G638510 PE=4 SV=1)

HSP 1 Score: 1008.4 bits (2606), Expect = 3.2e-291
Identity = 509/540 (94.26%), Postives = 521/540 (96.48%), Query Frame = 1

Query: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MARL+ N+QFSFETPENEA ITKKIP SERTNKYPNWYSPDTGIC SVHGYRKLPSDPFL
Sbjct: 1   MARLI-NNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS QFQHNGHSALIDS TGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIF+PIIILGALYLGAV+TTMFPQSSSSEIKKRITDCNVRLAF ITQKIKNFEALGIKT
Sbjct: 121 NSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKT 180

Query: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTNF+LMR MGFSSFYELIS GSDLIK+PVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPIL AL+RTAEKIGV RFRSLKQVSCGAAASSKKTID+F
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNF 360

Query: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           V AL HVDFIQGYGMTESTA+GTRGFN K  RNYLSVGLLAPNTEAKVVDWVTGSSMPPG
Sbjct: 361 VHALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEAT  T+DQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540
           GFQIAPTDLEAVVI HPEVLDTAVAAAKDEECGEIPVAFVVKKPGSAL+QKDVVDYVAQQ
Sbjct: 481 GFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQ 539

BLAST of MELO3C022770 vs. TrEMBL
Match: W9SFR0_9ROSA (4-coumarate--CoA ligase-like 6 OS=Morus notabilis GN=L484_022816 PE=4 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 9.6e-187
Identity = 329/517 (63.64%), Postives = 413/517 (79.88%), Query Frame = 1

Query: 27  LSERT--NKYPNWYSPDTGICHSVHGYRKLPSDPFLDVVSFIFSVQFQHNGHSALIDSLT 86
           LS +T   ++P+WYSP+TGICHS +     PSDPFLDVVSFIFS Q  HNG SAL+DS +
Sbjct: 4   LSNKTLDTQFPHWYSPETGICHSTNRPITFPSDPFLDVVSFIFSKQ--HNGVSALVDSSS 63

Query: 87  GNS-ISYKELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMF 146
           G+S ISY EL  +VKSMASGLH++G+S+ DVVLL+LPNS+++P+I+LG +YLGA++TTM 
Sbjct: 64  GSSSISYSELHRLVKSMASGLHSMGVSKNDVVLLLLPNSVYFPVILLGVVYLGAIVTTMN 123

Query: 147 PQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKTIGVPENTNFDLMRPMGFSSFYEL 206
           P S+ +E+KK++ D    LAFT+ +K++   A  +  I VPEN N        F  FY+L
Sbjct: 124 PLSTVTEVKKQVADSKACLAFTLPEKVELLRAFRVGAIAVPENFNSSQKPMSSFREFYKL 183

Query: 207 ISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLP 266
           +S   D   RP+IRQ+DTAAI++SSGTTG SKGV+L+H NFI+ ++LFVRFEASQYEY  
Sbjct: 184 VSCEFDFAPRPLIRQQDTAAIMYSSGTTGASKGVVLTHGNFIAMVDLFVRFEASQYEYPR 243

Query: 267 TKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPI 326
           ++NVYLA +PMFHIYGL++FVMGL+SLGS++VVM KFDV D+VK I+ +KVTHFPVVPP+
Sbjct: 244 SENVYLAVLPMFHIYGLALFVMGLLSLGSTIVVMRKFDVNDMVKTIETYKVTHFPVVPPM 303

Query: 327 LIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIGT 386
           LIAL++ A+ +G + FRSLKQVS GAA  S K I+DF+  L HVDFIQGYGMTESTA+GT
Sbjct: 304 LIALTKCAKVVGGKGFRSLKQVSSGAAPISGKIIEDFLHTLPHVDFIQGYGMTESTAVGT 363

Query: 387 RGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEAT 446
           RGFN KK R + SVGLL PN EAKV+D  TGS +PPG  GEL LRGP  MKGYLNN  AT
Sbjct: 364 RGFNTKKCRKFSSVGLLGPNMEAKVMDLNTGSFLPPGSCGELWLRGPAVMKGYLNNAVAT 423

Query: 447 MSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTA 506
           MS ID++ WL TGDIVYFD+DGYL+VVDRLKE+IKYKG+QIAP DLEAV+ +HPE+ D A
Sbjct: 424 MSAIDKDGWLRTGDIVYFDQDGYLHVVDRLKEIIKYKGYQIAPADLEAVLASHPEIRDVA 483

Query: 507 VAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 541
           VAAA+D + GE+PVAFVV+K GS+LSQ+ V+DYVA+Q
Sbjct: 484 VAAAEDADSGEVPVAFVVRKHGSSLSQEAVMDYVARQ 518

BLAST of MELO3C022770 vs. TrEMBL
Match: S4W608_CHOAR (Ca4CL9 OS=Chosenia arbutifolia PE=2 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 1.2e-186
Identity = 328/518 (63.32%), Postives = 404/518 (77.99%), Query Frame = 1

Query: 23  KKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFLDVVSFIFSVQFQHNGHSALIDS 82
           +K+  +     +P+WYSP+TGI  S H    LP+DPFLDVVSFIFS    HNG +ALIDS
Sbjct: 2   EKLTATASKTTFPDWYSPETGIYSSKHPTIPLPADPFLDVVSFIFS-HHNHNGLTALIDS 61

Query: 83  LTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTM 142
            +G SISY ++ P+VKS+ASGL N+GI QGDVVLL+LPNSI +PI+ LG LYLG +++TM
Sbjct: 62  SSGFSISYSKILPLVKSVASGLRNMGIKQGDVVLLLLPNSIHFPIVFLGVLYLGGIVSTM 121

Query: 143 FPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKTIGVPENTNFDLMRPMGFSSFYE 202
            P SS  E+KKRI DCN R+AF   +K+  F+ LGI  IGV EN N D  +   F +FY 
Sbjct: 122 NPLSSEPEVKKRIADCNARIAFVELKKVGKFQPLGILVIGVQENVNLD-EKSNDFEAFYM 181

Query: 203 LISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYL 262
            + S  D+  +PVIRQ+DTAAI++SSGTT  SKGV+L+HRNFIS +ELFV+FEASQYEY 
Sbjct: 182 RLYSKGDVGVKPVIRQQDTAAIMYSSGTTAASKGVVLTHRNFISMVELFVKFEASQYEYS 241

Query: 263 PTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPP 322
            T NVYLA +PMFHIYGLS+FV+GL+SLGSS+VVM KFDV + VK IDR+ VTHFPVVPP
Sbjct: 242 STDNVYLAVLPMFHIYGLSLFVVGLLSLGSSIVVMRKFDVSETVKVIDRYGVTHFPVVPP 301

Query: 323 ILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIG 382
           IL AL+RTA+ +     + LK VSCGAA    KTI DFV+ L HVDFIQGYG+TESTA+G
Sbjct: 302 ILTALTRTAKGVCGNSLKCLKVVSCGAAPLFAKTIQDFVEVLPHVDFIQGYGLTESTAVG 361

Query: 383 TRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEA 442
           TRG N +K + Y S+GLLAPN EAKVVDW+TG+ +PPG +GEL +RGPG MK YLN+ EA
Sbjct: 362 TRGLNTEKFKKYSSIGLLAPNIEAKVVDWITGTLLPPGGSGELWIRGPGVMKEYLNDGEA 421

Query: 443 TMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDT 502
           T  TID++ WLHTGD+VY D DGYLYVVDRLKE+IKYKGFQIAP DLEAV+I+H E+LD 
Sbjct: 422 TALTIDKDGWLHTGDVVYTDHDGYLYVVDRLKEIIKYKGFQIAPADLEAVLISHCEILDA 481

Query: 503 AVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 541
           AV  A D+ECGEIPVAFVVK+ GS ++++ +++YVA Q
Sbjct: 482 AVIPAIDKECGEIPVAFVVKRKGSMVTKEAIINYVADQ 517

BLAST of MELO3C022770 vs. TrEMBL
Match: A0A061G6U1_THECC (Acyl:coa ligase OS=Theobroma cacao GN=TCM_016640 PE=4 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 4.0e-185
Identity = 330/534 (61.80%), Postives = 411/534 (76.97%), Query Frame = 1

Query: 7   NDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFLDVVSFI 66
           N  FSF+  E+            +T ++P+WYSP+TGI  S H    LP+D FLD+VSFI
Sbjct: 6   NSHFSFQRSES------------KTTRFPDWYSPETGIYSSKHSSISLPTDHFLDIVSFI 65

Query: 67  FSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFYP 126
           FS   QH+G +ALIDS +G S SY +L P+++SMASGLH+LG+SQGDVVLL+LPNS+ +P
Sbjct: 66  FS--HQHDGVTALIDSSSGYSTSYAKLLPLIQSMASGLHHLGVSQGDVVLLLLPNSVHFP 125

Query: 127 IIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKTIGVPEN 186
           II L  LYLGA++TTM P +S  EIKK+I D +VR AFT+  K+   + LG+  IGVPEN
Sbjct: 126 IIFLSVLYLGAIVTTMNPLTSMLEIKKQIADSSVRFAFTLLDKVDKLKNLGVHAIGVPEN 185

Query: 187 TNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFIS 246
            N D  +   F  F++LI    D   RPVIRQ+DTAAI++SSGTTGVSKGV+L+H NFI+
Sbjct: 186 LNLDSEKT-DFLLFHKLIGGQFDKAPRPVIRQQDTAAIMYSSGTTGVSKGVVLTHGNFIA 245

Query: 247 TIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVV 306
            +ELFVRFEASQYEY  +KNVYLA +PMFHIYGLS+FV+GL+SLG+S+VVM +F+  ++V
Sbjct: 246 MVELFVRFEASQYEYSSSKNVYLAVLPMFHIYGLSLFVVGLLSLGTSIVVMRRFNATEMV 305

Query: 307 KAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDFVQALSH 366
           + ID++ VTHFPVVPPIL  L+  A+ +     +SLKQVSCGAA  S+K I+DFVQAL H
Sbjct: 306 RVIDKYGVTHFPVVPPILRTLAMRAKGVCENSLKSLKQVSCGAAPLSRKAIEDFVQALPH 365

Query: 367 VDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELL 426
           VDFIQGYGMTESTA+G RGFNN+K   Y SVGLLAPN +AKVVDW +GSS+PP   GEL 
Sbjct: 366 VDFIQGYGMTESTAVGNRGFNNEKHHKYSSVGLLAPNMQAKVVDWNSGSSLPPDSHGELW 425

Query: 427 LRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAP 486
           LRGPG M+GYLNN EAT  TID++ WL TGDIV FD DGY+YV DRLKE+IKYKG+QIAP
Sbjct: 426 LRGPGLMQGYLNNVEATRMTIDKDGWLRTGDIVCFDEDGYMYVSDRLKEIIKYKGYQIAP 485

Query: 487 TDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 541
            DLEA++I+HPE+LD AV  A DEE GEIPVAFVV++ GS L+Q  V+D+VA+Q
Sbjct: 486 ADLEAILISHPEILDAAVIPAIDEEAGEIPVAFVVRRQGSTLTQGAVMDFVAKQ 524

BLAST of MELO3C022770 vs. TrEMBL
Match: A0A0D2S1Q6_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_004G180900 PE=4 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 6.9e-185
Identity = 327/511 (63.99%), Postives = 402/511 (78.67%), Query Frame = 1

Query: 30  RTNKYPNWYSPDTGICHSVHGYRKLPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSIS 89
           +T   P+WYSP+T I  S +    LP+DPFLD+VSFIFS   QH+G +ALIDS +G SIS
Sbjct: 15  KTTHLPDWYSPETRIYSSTYSSISLPTDPFLDIVSFIFS--HQHDGVTALIDSSSGYSIS 74

Query: 90  YKELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSS 149
           Y +L P+V+SMASGLH+LG+SQGDVVLL+LPNS+ +PII    LYLGAV+TTM P S+  
Sbjct: 75  YSKLLPLVQSMASGLHHLGVSQGDVVLLLLPNSVNFPIIFFSVLYLGAVVTTMNPLSNMM 134

Query: 150 EIKKRITDCNVRLAFTITQKIKNFEALGIKTIGVPENTNFDLMRPMGFSSFYELISSGSD 209
           E+KK+I DC VR AFT+ + +   + LG+  IGVPEN + D  +  GF SF +LI     
Sbjct: 135 EVKKQIVDCGVRFAFTLLENVDKLQKLGVHAIGVPENMDLDSEKT-GFLSFRKLIGGQFG 194

Query: 210 LIKRPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYL 269
              RPVIRQ+DTAAI++SSGTTGVSKGV+L+H NFI+T ELFVRFEASQYEY  +KNVYL
Sbjct: 195 KAPRPVIRQQDTAAIMYSSGTTGVSKGVVLTHGNFIATTELFVRFEASQYEYSSSKNVYL 254

Query: 270 AAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIALSR 329
           A +PMFHIYGLS+FV+GL+SLGSS+V+M +FD  ++VK ID + VTHFPVVPPIL  L+ 
Sbjct: 255 AVLPMFHIYGLSLFVVGLLSLGSSIVIMRRFDASELVKVIDDYGVTHFPVVPPILTTLTM 314

Query: 330 TAEKIGVRRFRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGFNNK 389
            A+ +     +SLKQVSCGAA  S KTI+DFVQAL HVDFIQGYGMTESTA+GTRGFNN+
Sbjct: 315 RAKHVCENSLQSLKQVSCGAAPLSGKTIEDFVQALPHVDFIQGYGMTESTAVGTRGFNNE 374

Query: 390 KTRNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDQ 449
           K  NY S+GLLAPN +AKVVDW +GSS+PPG  GEL LRGP  M+ Y+NN EAT  TID+
Sbjct: 375 KHHNYSSIGLLAPNMQAKVVDWNSGSSLPPGFHGELWLRGPAIMQRYINNVEATNMTIDK 434

Query: 450 ENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAAAKD 509
           + WL TGDIV FD +GYLY+ DRLKE+IKYKG+QIAP DLEAV+I+HPE+LD AV +A D
Sbjct: 435 DGWLRTGDIVCFDGEGYLYLSDRLKEIIKYKGYQIAPADLEAVLISHPEILDAAVTSAAD 494

Query: 510 EECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 541
           E CGEIPVAFVV++ GS L++  V+D+VA Q
Sbjct: 495 EVCGEIPVAFVVRRHGSTLTKGAVIDFVANQ 522

BLAST of MELO3C022770 vs. TAIR10
Match: AT4G19010.1 (AT4G19010.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 607.8 bits (1566), Expect = 6.3e-174
Identity = 300/507 (59.17%), Postives = 393/507 (77.51%), Query Frame = 1

Query: 35  PNWYSPDTGICHSVHGYRKLPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYKELF 94
           P W+S  TGI  S      LP DP LD VS +FS   +H+G +ALIDSLTG SIS+ EL 
Sbjct: 22  PFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFS--HKHHGDTALIDSLTGFSISHTELQ 81

Query: 95  PMVKSMASGL-HNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKK 154
            MV+SMA+G+ H LG+ QGDVV L+LPNS+++P+I L  + LGA++TTM P SS  EIKK
Sbjct: 82  IMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSSSLGEIKK 141

Query: 155 RITDCNVRLAFTITQKIKNFEALGIKTIGVPENTNFDLMRPMGFSSFYELISSGSDLIKR 214
           ++++C+V LAFT T+ ++   +LG+  I V E+ +FD +R +    FY ++      + +
Sbjct: 142 QVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIR-IENPKFYSIMKESFGFVPK 201

Query: 215 PVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAAIP 274
           P+I+Q+D AAI++SSGTTG SKGV+L+HRN I+++ELFVRFEASQYEY  + NVYLAA+P
Sbjct: 202 PLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALP 261

Query: 275 MFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIALSRTAEK 334
           + HIYGLS+FVMGL+SLGS++VVM +FD  DVV  I+RFK+THFPVVPP+L+AL++ A+ 
Sbjct: 262 LCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLMALTKKAKG 321

Query: 335 IGVRRFRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGFNNKKTRN 394
           +    F+SLKQVS GAA  S+K I+DF+Q L HVD IQGYGMTESTA+GTRGFN++K   
Sbjct: 322 VCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRGFNSEKLSR 381

Query: 395 YLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDQENWL 454
           Y SVGLLAPN +AKVVDW +GS +PPG  GEL ++GPG MKGYLNNP+AT  +I +++WL
Sbjct: 382 YSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQMSIVEDSWL 441

Query: 455 HTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECG 514
            TGDI YFD DGYL++VDR+KE+IKYKGFQIAP DLEAV+++HP ++D AV AA +EECG
Sbjct: 442 RTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECG 501

Query: 515 EIPVAFVVKKPGSALSQKDVVDYVAQQ 541
           EIPVAFVV++  + LS++DV+ YVA Q
Sbjct: 502 EIPVAFVVRRQETTLSEEDVISYVASQ 525

BLAST of MELO3C022770 vs. TAIR10
Match: AT1G20510.1 (AT1G20510.1 OPC-8:0 CoA ligase1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.0e-110
Identity = 212/491 (43.18%), Postives = 320/491 (65.17%), Query Frame = 1

Query: 54  LPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGD 113
           LP +P LDV +FI S    H G  A ID+ TG ++++ EL+  V+S+A  L  +GI +G 
Sbjct: 26  LPPNPSLDVTTFISSQA--HRGRIAFIDASTGQNLTFTELWRAVESVADCLSEIGIRKGH 85

Query: 114 VVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNF 173
           VVLL+ PNSI +P++ L  + LGA+ITT  P ++S+EI K+I D N  LAFT +Q +   
Sbjct: 86  VVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKI 145

Query: 174 EALGIKT-IGVPENTNFDLMRPMG-FSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTT 233
            A   K  I + +    D +  +       +   SG+ + +R  + Q+DTA +L+SSGTT
Sbjct: 146 SAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKEPSGNRVKER--VDQDDTATLLYSSGTT 205

Query: 234 GVSKGVMLSHRNFISTIELFV-RFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSL 293
           G+SKGV+ SHRN I+ ++  V RF +   E       ++  +PMFHIYGL+ F  GL++ 
Sbjct: 206 GMSKGVISSHRNLIAMVQTIVNRFGSDDGEQR-----FICTVPMFHIYGLAAFATGLLAY 265

Query: 294 GSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRR-FRSLKQVSCGA 353
           GS+++V+SKF++ +++ AI +++ T  P+VPPIL+A+   A++I  +    S+  V CG 
Sbjct: 266 GSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSSMHTVLCGG 325

Query: 354 AASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVV 413
           A  SK+  + F +    V  +QGYG+TEST IG      +++R Y + G L+ + E ++V
Sbjct: 326 APLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRIV 385

Query: 414 DWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYV 473
           D VTG  + P +TGEL L+GP  MKGY +N EAT ST+D E WL TGD+ Y D DG+++V
Sbjct: 386 DPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFV 445

Query: 474 VDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALS 533
           VDRLKE+IKYKG+Q+AP +LEA+++ HPE+ D AV    D+E G+ P+A+VV+K GS+LS
Sbjct: 446 VDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLS 505

Query: 534 QKDVVDYVAQQ 541
           +K ++++VA+Q
Sbjct: 506 EKTIMEFVAKQ 507

BLAST of MELO3C022770 vs. TAIR10
Match: AT4G05160.1 (AT4G05160.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 381.3 bits (978), Expect = 9.7e-106
Identity = 207/510 (40.59%), Postives = 316/510 (61.96%), Query Frame = 1

Query: 43  GICHSVHGYRKLPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMAS 102
           GI  S+     LP DP   +VSF+F     +    A+ DS TG+S+++ +L   V  +A 
Sbjct: 10  GIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAH 69

Query: 103 GLHNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRL 162
           G H LGI + DVVL+  PNS  +P+  L    +G V TT  P  + +E+ K+I D N ++
Sbjct: 70  GFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKI 129

Query: 163 AFTITQ---KIKNFEA----LGIK-TIGVPENTNFDLMRPMGFSSFYELISSGSDLIKRP 222
             ++ Q   KIK F+     LG K T+ +P  +N  ++      SF  ++     + + P
Sbjct: 130 IISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKIL------SFDNVMELSEPVSEYP 189

Query: 223 V--IRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAAI 282
              I+Q DTAA+L+SSGTTG SKGV L+H NFI+   +    +    EY     V+L  +
Sbjct: 190 FVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEY---HGVFLCFL 249

Query: 283 PMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIALSRTAE 342
           PMFH++GL++     +  G+++V M++F+++ V+K I++F+VTH  VVPP+ +ALS+ + 
Sbjct: 250 PMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSI 309

Query: 343 KIGVRRF--RSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGFNNKK 402
              V++F   SLK +  GAA   K  +++  + + +V  +QGYGMTE+  I +       
Sbjct: 310 ---VKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLG 369

Query: 403 TRNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDQE 462
            RN  S G+LAP  EA++V   TG S PP + GE+ +RGP  MKGYLNNP+AT  TID++
Sbjct: 370 KRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKK 429

Query: 463 NWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAAAKDE 522
           +W+HTGD+ YF+ DG LYVVDR+KE+IKYKGFQ+AP +LE ++++HP++LD  V    DE
Sbjct: 430 SWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDE 489

Query: 523 ECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 541
           E GE+P+AFVV+ P S+++++D+  ++A+Q
Sbjct: 490 EAGEVPIAFVVRSPNSSITEQDIQKFIAKQ 507

BLAST of MELO3C022770 vs. TAIR10
Match: AT1G20480.1 (AT1G20480.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 375.9 bits (964), Expect = 4.1e-104
Identity = 211/514 (41.05%), Postives = 312/514 (60.70%), Query Frame = 1

Query: 38  YSPDTGICHSVHGYRKLPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMV 97
           +   T I +S      LP + FLDV SFI S    H G +  +D++TG  +S+ EL+  V
Sbjct: 27  FCESTSIFYSKREPMALPPNQFLDVTSFIASQP--HRGKTVFVDAVTGRRLSFPELWLGV 86

Query: 98  KSMASGLHNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITD 157
           + +A  L+ LG+ +G+VV+++ PNSI +PI+ L  + LGA+ITT  P ++S EI K+I D
Sbjct: 87  ERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEISKQIGD 146

Query: 158 CNVRLAFTITQKIKNFEALG--------IKTIGVPENTNFDLMRPMG-FSSFYELISSGS 217
               LAFT  + +    A          +    VP  +  D ++ +G   +  E   S S
Sbjct: 147 SRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIETEPSES 206

Query: 218 DLIKRPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELF-VRFEASQYEYLPTKNV 277
            + +R  + Q+DTAA+L+SSGTTG SKGVMLSHRN I+ ++ +  RF   Q         
Sbjct: 207 RVKQR--VNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRARFGLEQRT------- 266

Query: 278 YLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIAL 337
            +  IPM HI+G   F  GL++LG ++VV+ KFD+  ++ A++  + ++  +VPPI++A+
Sbjct: 267 -ICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAM 326

Query: 338 SRTAEKIGVRR-FRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGF 397
              A +I  +    SL  V  G A  S++  + FV+    V  +QGYG+TESTAI    F
Sbjct: 327 VNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMF 386

Query: 398 NNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATMST 457
           N ++T+ Y + GLLAPN E K+VD  TG  +   +TGEL +R P  MKGY  N EAT ST
Sbjct: 387 NKEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATAST 446

Query: 458 IDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAA 517
           ID E WL TGD+ Y D DG+++VVDRLKE+IK  G+Q+AP +LEA+++AHPE+ D AV  
Sbjct: 447 IDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIP 506

Query: 518 AKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 541
             D + G+ P+A++V+K GS LS+ +++ +VA+Q
Sbjct: 507 IPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQ 528

BLAST of MELO3C022770 vs. TAIR10
Match: AT1G20500.1 (AT1G20500.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 360.5 bits (924), Expect = 1.8e-99
Identity = 202/517 (39.07%), Postives = 307/517 (59.38%), Query Frame = 1

Query: 40  PDTGICHSVHGYRK------LPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYKEL 99
           P +G C S   +        LP +   DV +FI S    H G +A ID+ TG  +++ +L
Sbjct: 12  PRSGFCKSNSTFYSKRQPLSLPPNLSRDVTTFISSQP--HRGKTAFIDAATGQCLTFSDL 71

Query: 100 FPMVKSMASGL-HNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIK 159
           +  V  +A  L H +GI +GDVVL++ PNSIF P++ L  + LGAV TT    ++S EI 
Sbjct: 72  WRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEIS 131

Query: 160 KRITDCNVRLAFTITQKIKNFEALGIKTIGVPENTNFDLMRPMGFSSFYELISSGSDLIK 219
           K+I D N  L FT T+++     + I  +   +    +L      +S   ++   S+++K
Sbjct: 132 KQIADSNPTLVFT-TRQLAPKLPVAISVVLTDDEVYQEL------TSAIRVVGILSEMVK 191

Query: 220 --------RPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPT 279
                   R  + Q+DTA +L+SSGTTG SKGV+ SHRN  + +  F+       + L  
Sbjct: 192 KEPSGQRVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFIS------DNLKR 251

Query: 280 KNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPIL 339
            ++++  +PMFH YGL  F MG ++LGS+VV++ +F + D++ A+++ + T   + PP+L
Sbjct: 252 DDIFICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVL 311

Query: 340 IALSRTAEKIGVRR-FRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIGT 399
           +A+   A+ I  +    SLK V CG A  SK+  + F++    VD +QGY +TES   G 
Sbjct: 312 VAMINDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGGA 371

Query: 400 RGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEAT 459
              + +++R Y + G L  + EA++VD  TG  M   +TGEL L+GP   KGY  N EAT
Sbjct: 372 FTNSAEESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEAT 431

Query: 460 MSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTA 519
             TI+ E WL TGD+ Y D DG+L+VVDRLKE+IKYKG+Q+ P +LEA++I HP++LD A
Sbjct: 432 NETINLEGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDAA 491

Query: 520 VAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 541
           V    D+E G+ P+A+VV+K  S LS+K V+D++++Q
Sbjct: 492 VIPFPDKEAGQYPMAYVVRKHESNLSEKQVIDFISKQ 513

BLAST of MELO3C022770 vs. NCBI nr
Match: gi|659120993|ref|XP_008460452.1| (PREDICTED: 4-coumarate--CoA ligase-like 6 [Cucumis melo])

HSP 1 Score: 1066.2 bits (2756), Expect = 1.8e-308
Identity = 540/540 (100.00%), Postives = 540/540 (100.00%), Query Frame = 1

Query: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540
           GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ
Sbjct: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540

BLAST of MELO3C022770 vs. NCBI nr
Match: gi|449453328|ref|XP_004144410.1| (PREDICTED: 4-coumarate--CoA ligase-like 6 [Cucumis sativus])

HSP 1 Score: 1008.4 bits (2606), Expect = 4.6e-291
Identity = 509/540 (94.26%), Postives = 521/540 (96.48%), Query Frame = 1

Query: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MARL+ N+QFSFETPENEA ITKKIP SERTNKYPNWYSPDTGIC SVHGYRKLPSDPFL
Sbjct: 1   MARLI-NNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS QFQHNGHSALIDS TGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIF+PIIILGALYLGAV+TTMFPQSSSSEIKKRITDCNVRLAF ITQKIKNFEALGIKT
Sbjct: 121 NSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKT 180

Query: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTNF+LMR MGFSSFYELIS GSDLIK+PVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPIL AL+RTAEKIGV RFRSLKQVSCGAAASSKKTID+F
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNF 360

Query: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           V AL HVDFIQGYGMTESTA+GTRGFN K  RNYLSVGLLAPNTEAKVVDWVTGSSMPPG
Sbjct: 361 VHALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEAT  T+DQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540
           GFQIAPTDLEAVVI HPEVLDTAVAAAKDEECGEIPVAFVVKKPGSAL+QKDVVDYVAQQ
Sbjct: 481 GFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQ 539

BLAST of MELO3C022770 vs. NCBI nr
Match: gi|1009166294|ref|XP_015901516.1| (PREDICTED: 4-coumarate--CoA ligase-like 6 [Ziziphus jujuba])

HSP 1 Score: 677.6 bits (1747), Expect = 1.8e-191
Identity = 344/538 (63.94%), Postives = 423/538 (78.62%), Query Frame = 1

Query: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MA  L+N   S  + E E+  +   P       +P+WYSP+TGI  S +    LPSDPFL
Sbjct: 19  MATSLNNAFTSILSEEKESEKSLHAP-------FPHWYSPETGIYSSRNPSVHLPSDPFL 78

Query: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           D VSFIFS +  HNG SALIDS +G+SISY EL+ +V+SMASGLH +GISQGDVVL++LP
Sbjct: 79  DSVSFIFSNR--HNGVSALIDSSSGSSISYSELYRLVESMASGLHYMGISQGDVVLVLLP 138

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           N I+YPII LG LYLG V+T M P S+ SEIKK+I DCNV  AFT  +K++  + LG+  
Sbjct: 139 NCIYYPIIFLGVLYLGGVVTAMNPSSTVSEIKKQIADCNVCFAFTDPEKVEKLQVLGVPA 198

Query: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPEN NFD  +   FS+FY+LIS    ++ RP+I+Q DTAAI+ SSGTTGVSKGV+L+
Sbjct: 199 IGVPENVNFD-SKATCFSAFYKLISCQFSMVPRPMIKQLDTAAIMHSSGTTGVSKGVVLT 258

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFI+ +ELFVRFEASQY+Y   KNVYLA +PMFHIYGLS+F+MGL+SLGS+VVVM KF
Sbjct: 259 HRNFIAMVELFVRFEASQYDYSSLKNVYLAIVPMFHIYGLSLFIMGLLSLGSTVVVMKKF 318

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360
           ++ + VKAIDR+KVTHFP+VPP+L AL+R+A+ +  + FRSLKQVSCGAA  S + I++F
Sbjct: 319 NINEAVKAIDRYKVTHFPLVPPMLTALTRSAKDVDWKSFRSLKQVSCGAAPLSWRIIEEF 378

Query: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           VQ   HVDFIQGYG+TESTA+GTRGFN +K RNY S+GLLAPN +AKVVD   GS +PPG
Sbjct: 379 VQTFPHVDFIQGYGLTESTAVGTRGFNTEKFRNYSSIGLLAPNMQAKVVDSNIGSFLPPG 438

Query: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           K+GEL LRGP  M+GYLNN EATM ++D++ W+HTGDIVYFD DGYLY+ DRLKEVIKYK
Sbjct: 439 KSGELWLRGPAVMQGYLNNAEATMLSVDKDGWMHTGDIVYFDGDGYLYLFDRLKEVIKYK 498

Query: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVA 539
           GFQIAP DLEAV+I HPE+ D A+A   D+E GEIPVAFVVKK  S+LS+ DV+DYVA
Sbjct: 499 GFQIAPADLEAVLICHPEIEDVAIAGTMDQESGEIPVAFVVKKHRSSLSEADVMDYVA 546

BLAST of MELO3C022770 vs. NCBI nr
Match: gi|764589967|ref|XP_004301643.2| (PREDICTED: 4-coumarate--CoA ligase-like 6 [Fragaria vesca subsp. vesca])

HSP 1 Score: 671.8 bits (1732), Expect = 1.0e-189
Identity = 330/512 (64.45%), Postives = 406/512 (79.30%), Query Frame = 1

Query: 29  ERTNKYPNWYSPDTGICHSVHGYRKLPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSI 88
           ++  ++P W+SP+TGI  S H    LPSDPFLDVVSFIFS   +H+G SALIDS +G SI
Sbjct: 34  QQQQQHPWWFSPETGIYSSKHPNVNLPSDPFLDVVSFIFS--HKHSGVSALIDSSSGFSI 93

Query: 89  SYKELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSS 148
           SY +L+ +V+SMASGL  +G+SQGDVVLL+LPNS++YPI+ LG LY+GAV+TTM P SS 
Sbjct: 94  SYSKLYSLVESMASGLIKMGVSQGDVVLLLLPNSVYYPIVFLGVLYIGAVVTTMNPLSSV 153

Query: 149 SEIKKRITDCNVRLAFTITQKIKNFEALGIKTIGVPENTNFDLMRPMGFSSFYELISSGS 208
            EIKK+I DCN    FT  + +   +AL I  I VP N   D  + M FS FY+L+ S  
Sbjct: 154 VEIKKQIKDCNACFGFTGYENVDKLQALSIPAIAVPGNVVLDSKKEM-FSVFYQLVDSEV 213

Query: 209 DLIKRPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVY 268
           DL  RPVI+Q+DTAAIL+SSGTTGVSKGV+++H N I+T+ELFVRFEASQYEY   KNVY
Sbjct: 214 DLALRPVIKQQDTAAILYSSGTTGVSKGVLITHGNLIATVELFVRFEASQYEYSSLKNVY 273

Query: 269 LAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIALS 328
           L+ +P+FHIYGL++ V+GL+SLGSS+V+M KFDV +VV AIDR+KVTHFPVVPPIL AL+
Sbjct: 274 LSVLPLFHIYGLALMVVGLLSLGSSIVIMKKFDVNEVVSAIDRYKVTHFPVVPPILTALT 333

Query: 329 RTAEKIGVRRFRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGFNN 388
           + A+ +G +  +SLKQVSCGAA +S K I++ VQ L HVDFIQGYGMTE+TA+GTRGFN 
Sbjct: 334 KIAQDVGAQSLQSLKQVSCGAAPTSMKLIEELVQTLPHVDFIQGYGMTETTAVGTRGFNT 393

Query: 389 KKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATMSTID 448
           +K R Y S+GLLAPN +AKVVDW TGS +PP   GEL LRGP  M+GYLNN  ATMSTID
Sbjct: 394 EKIRKYSSIGLLAPNMQAKVVDWNTGSFLPPASIGELWLRGPSIMRGYLNNARATMSTID 453

Query: 449 QENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAAAK 508
            + WLHTGDI YFD DGYLY+ DR+KE+IKYKGFQIAP DLEAV+I+HPE+LD  V  A 
Sbjct: 454 NDGWLHTGDISYFDEDGYLYICDRIKEIIKYKGFQIAPADLEAVLISHPEILDVGVTGAT 513

Query: 509 DEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 541
           DEECGE+PVAFVV+K  S LSQ+D++DYVA+Q
Sbjct: 514 DEECGEVPVAFVVRKHDSELSQEDIMDYVARQ 542

BLAST of MELO3C022770 vs. NCBI nr
Match: gi|1000966856|ref|XP_015574447.1| (PREDICTED: LOW QUALITY PROTEIN: 4-coumarate--CoA ligase-like 6 [Ricinus communis])

HSP 1 Score: 663.3 bits (1710), Expect = 3.6e-187
Identity = 327/509 (64.24%), Postives = 401/509 (78.78%), Query Frame = 1

Query: 32  NKYPNWYSPDTGICHSVHGYRKLPSDPFLDVVSFIFSVQFQHNGHSALIDSLTGNSISYK 91
           N  P WY P TGI  S H    LP+DPFLDVVSFIFS   +HNG +A IDS +G SI Y 
Sbjct: 15  NNNPEWYCPQTGIYTSKHPTVSLPTDPFLDVVSFIFS--HKHNGVTAFIDSFSGFSIPYS 74

Query: 92  ELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEI 151
           +LFP+V SMASGL N+G+ QGDVVLL+LPNSI++PI+ LG LYLGAV+TTM P S+S EI
Sbjct: 75  DLFPLVNSMASGLQNMGLKQGDVVLLLLPNSIYFPILFLGVLYLGAVVTTMNPLSTSLEI 134

Query: 152 KKRITDCNVRLAFTITQKIKNFEALGIKTIGVPENTNFDLMRPMGFSSFYELISSGSDLI 211
           +K+I++CN  +AF++ +K    ++LGI  + VPEN    L     F +FY L+    DL 
Sbjct: 135 EKQISNCNACIAFSVLEKAAKLQSLGIPVVSVPENVT-SLKANKAFDAFYMLLYGNVDLC 194

Query: 212 KRPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAA 271
            RP+IRQEDTAAIL+SSGTTG SKGV+L+HRNFIS +ELFVRFEASQY+Y  +KNV+LA 
Sbjct: 195 LRPIIRQEDTAAILYSSGTTGSSKGVVLTHRNFISMVELFVRFEASQYDYSSSKNVFLAV 254

Query: 272 IPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILIALSRTA 331
           +PMFH+YGLS+FVMGL+SLGSS+VVM KFD  + VKAID  +VTHFPVVPP+L  L++ A
Sbjct: 255 LPMFHVYGLSLFVMGLLSLGSSIVVMRKFDANEXVKAIDMHEVTHFPVVPPMLQVLTKKA 314

Query: 332 EKIGVRRFRSLKQVSCGAAASSKKTIDDFVQALSHVDFIQGYGMTESTAIGTRGFNNKKT 391
           + +  +  +SLKQVSCGAA    KTI DFV+ L +VDFIQGYGMTESTA+GTRGFN K  
Sbjct: 315 KSVYGKSLKSLKQVSCGAAPLFGKTIQDFVETLPYVDFIQGYGMTESTAVGTRGFNTKNF 374

Query: 392 RNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDQEN 451
           + Y S+GLLAPN EAKV+DWVTG  +PPG +GELL+RGP  MK YLNN EAT STID++ 
Sbjct: 375 QKYSSIGLLAPNIEAKVMDWVTGCFLPPGNSGELLIRGPAVMKEYLNNGEATASTIDKDG 434

Query: 452 WLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVIAHPEVLDTAVAAAKDEE 511
           WLHTGDIVY D DGYL++VDRLKE+IKYKGFQIAP DLEAV++ HP++LD AV AA D+E
Sbjct: 435 WLHTGDIVYIDHDGYLHIVDRLKEIIKYKGFQIAPADLEAVLVCHPDILDAAVTAAIDKE 494

Query: 512 CGEIPVAFVVKKPGSALSQKDVVDYVAQQ 541
           CGEIPVAFVVK+  S L+Q+D+++YVA Q
Sbjct: 495 CGEIPVAFVVKRLESMLTQEDIINYVAVQ 520