Bhi05G000002 (gene) Wax gourd

BLAST of Bhi05G000002 vs. Swiss-Prot
Match: sp|Q84P24|4CLL6_ARATH (4-coumarate--CoA ligase-like 6 OS=Arabidopsis thaliana OX=3702 GN=4CLL6 PE=2 SV=2)

HSP 1 Score: 644.8 bits (1662), Expect = 9.0e-184
Identity = 318/552 (57.61%), Postives = 429/552 (77.72%), Query Frame = 0

Query: 25  PRSEQTNKYPN-WYSPDTGICRSVHASRGVPSDPFLDVVSFIFSFKHNGNSAIIDSSTGS 84
           P+++ +++ P  W+S  TGI  S   S  +P DP LD VS +FS KH+G++A+IDS TG 
Sbjct: 12  PKTQTSHQNPPFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFSHKHHGDTALIDSLTGF 71

Query: 85  SISYKEMFHMVKSMASGL-QKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQ 144
           SIS+ E+  MV+SMA+G+    G+ QGDVV LVLPNS+++P+I L ++ LGA+VTTM P 
Sbjct: 72  SISHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPS 131

Query: 145 SSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQAIGVPEITNFDLMQPMGFSYFHELIS 204
           SS  EIKK++++C+V LAFT T+ ++   SLG+  I V E  +FD ++ +    F+ ++ 
Sbjct: 132 SSLGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIR-IENPKFYSIMK 191

Query: 205 SGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYLPTE 264
                  +P+I+Q+D AA+++SSGTTG SKGV+LTHRN I+++ELFVRFEASQYEY  + 
Sbjct: 192 ESFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSS 251

Query: 265 NVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIMT 324
           NVYLAA+P+ HIYGLS+FVMGLLSLGS++V+M +FD  +VV  I+RFK+THFPVVPP++ 
Sbjct: 252 NVYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLM 311

Query: 325 AMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGTRG 384
           A+ + A+ +    F+SLKQVS GAA L++K I+DF++  PHVD IQGYGMTESTA+GTRG
Sbjct: 312 ALTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRG 371

Query: 385 FNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEATMS 444
           FN++    Y+SVGLLAPN +AKVVDW SGSF+PPG  GEL ++GPG MKGYLNNP+AT  
Sbjct: 372 FNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQM 431

Query: 445 TIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTAVT 504
           +I +++WL TGDI YFD DGYL+++DR+KE+IKYKGFQIAP DLEAV+++HP ++D AVT
Sbjct: 432 SIVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVT 491

Query: 505 AAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGKVL 564
           AA +EECGEIPVAFVV++  + LS++DVI YVA QVAPY+KVRKVV   SIPKS  GK+L
Sbjct: 492 AAPNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKIL 551

Query: 565 RRELGKHLPSKL 575
           R+EL + L + +
Sbjct: 552 RKELKRILTNSV 562

BLAST of Bhi05G000002 vs. Swiss-Prot
Match: sp|Q6YYZ2|4CLL3_ORYSJ (4-coumarate--CoA ligase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL3 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.8e-147
Identity = 286/546 (52.38%), Postives = 369/546 (67.58%), Query Frame = 0

Query: 37  YSPDTGICRSVHASRGVPSDPFLDVVSFIFS---FKHNGNSAIIDSSTGSSISYKEMFHM 96
           YS  TGI  S+H    +P+DP L +V+ +F+       G   ++D++T S++S  ++  +
Sbjct: 28  YSAATGIYSSIHPPVALPTDPSLTLVAHLFARLPLADPGAPTLVDAATASAVSRADLRRL 87

Query: 97  VKSMASGL-QKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKRI 156
           V S+A+GL ++ G+ +G VVLL+LPNS+ +P+  L VL  GAV TTM P SS +EI  + 
Sbjct: 88  VASLAAGLRRRHGVRKGSVVLLLLPNSVAFPVSFLAVLAAGAVATTMNPSSSPAEIAAQA 147

Query: 157 NDCNVRLAFTITQKIKNFESLGIQAIGVPEITNFDLMQPMG------FSYFHELISSGSD 216
                 L             L    + VPEI +              F+ F  ++  G  
Sbjct: 148 RATGACLVLASRDGAARLPPLAAPVVLVPEILDHSAAXXXXXXXXRVFAAFRAMLDGGGG 207

Query: 217 LKQR---PVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYL-PTE 276
                  PV+ Q+D  A+L+SSGT+G SKGV+LTHRN I+  ELFVRFEASQY      E
Sbjct: 208 DGTETAVPVVGQDDAVAILYSSGTSGRSKGVVLTHRNLIAMTELFVRFEASQYHARGARE 267

Query: 277 NVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIMT 336
           NVY+AA+PM H+YGLS+F +GLLS+G++VV+M +FD  + V AI R+KVTH P+VPPIM 
Sbjct: 268 NVYMAALPMSHVYGLSLFAVGLLSIGATVVVMRRFDAGDAVAAIGRYKVTHMPLVPPIMA 327

Query: 337 AMARTAEKIGV--HRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGT 396
           AM R A   GV   +  SL QVSCGAA +    I +F++AFPHVDFIQGYGMTESTA+GT
Sbjct: 328 AMVRAAAAGGVPPSQVASLVQVSCGAAPITAALIHEFLQAFPHVDFIQGYGMTESTAVGT 387

Query: 397 RGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEAT 456
           RGFNT   + YTSVGLLAPN  AK+V   S S +PPG +GEL L GPG MKGYL++ +  
Sbjct: 388 RGFNTSKHKKYTSVGLLAPNMHAKIVHLESSSCLPPGFSGELWLHGPGIMKGYLSDDDDA 447

Query: 457 MSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTA 516
            +   K+ WL TGDI YFD DGYLY++ RLK+ IKYKGFQIAP DLE V++ HPE+LD A
Sbjct: 448 CTR--KDGWLRTGDIAYFDLDGYLYIVGRLKDTIKYKGFQIAPGDLEEVLIHHPEILDVA 507

Query: 517 VTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGK 567
           VT+A+DEE GEIPVAFVV++ GS LS K V++YVA+QVAPYK+VRKVVF E+IPKS AGK
Sbjct: 508 VTSAEDEEAGEIPVAFVVRRSGSNLSCKQVMEYVAKQVAPYKRVRKVVFVEAIPKSPAGK 567

BLAST of Bhi05G000002 vs. Swiss-Prot
Match: sp|Q336M7|4CLL2_ORYSJ (4-coumarate--CoA ligase-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL2 PE=3 SV=3)

HSP 1 Score: 472.2 bits (1214), Expect = 8.0e-132
Identity = 257/514 (50.00%), Postives = 344/514 (66.93%), Query Frame = 0

Query: 35  NWYSPDTGICRSVHASRGVPSDPFLDVVSFIFS-FKHNGNSAIIDSSTGSSISYKEMFHM 94
           ++YS  TG+  S+H    +PSDP L +V  +FS    + +S ++D+ T +++S  +   +
Sbjct: 14  SFYSAATGLYSSLHPPLPLPSDPSLSLVPHLFSHLPLHHHSLLVDAPTAATLSCADFRRL 73

Query: 95  VKSMASGL-QKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKRI 154
           V S+A+GL ++  I++G +VLL+LPNS+ +P+  L VL  GAV TTM P S+ +EI  R+
Sbjct: 74  VSSLAAGLRRRLHIARGSLVLLLLPNSLTFPVAFLAVLATGAVATTMNPSSAPAEIAARL 133

Query: 155 NDCNVRLAFTITQKIKNFESLGIQAIGVPEITNFDLMQPMGFSYFHELISSGSD--LKQR 214
            D    L    T        L    + VP+             +FH L+ +  +  ++  
Sbjct: 134 RDTAPSLVLASTHNAAKLPPLAAPLVLVPDTFQQQHDDDQFDFFFHALLETDPETPVEMG 193

Query: 215 PVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYLPTENVYLAAIP 274
             + Q+D AA+L+SSGT+G SKGV++THRN I+ +ELFVRFEASQY     +NVYLAA+P
Sbjct: 194 VGVGQDDAAAVLYSSGTSGRSKGVVVTHRNLIAMVELFVRFEASQYTRPARDNVYLAALP 253

Query: 275 MFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIMTAMARTAEK 334
           MFH+YGLS+F +GLLSLG +VV+M +F+V + VKAI ++KVTH P+VPPIM+A+ R    
Sbjct: 254 MFHVYGLSLFAVGLLSLGCTVVVMRRFNVDDAVKAIRKYKVTHLPLVPPIMSALLRANPP 313

Query: 335 IGVHRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGTRGFNTKNARN 394
           +      SL QVS GAA LN   I  F+ AFPHVDFIQGYGMTESTA+GTRGFNT   + 
Sbjct: 314 L---ELDSLLQVSSGAAPLNHTLIHHFLHAFPHVDFIQGYGMTESTAVGTRGFNTCKHKK 373

Query: 395 YTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMK------GYLNNPEATMSTI 454
           Y SVGLLAPN  AK+V   SGS +PPG  GEL L GP  MK      GYLN+ +   +  
Sbjct: 374 YASVGLLAPNMHAKIVHLESGSCLPPGSYGELWLHGPAIMKEFCFVTGYLNDDDDAFTR- 433

Query: 455 DKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTAVTAA 514
            K+ WL TGDI YFD DGYL+++ RLK+ IKYKGFQIAP DLEAV++ HPE++D AVT+ 
Sbjct: 434 -KDGWLRTGDIAYFDSDGYLFIVGRLKDTIKYKGFQIAPADLEAVLIRHPEIIDVAVTSD 493

Query: 515 KDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQV 539
           +DEE GEIPVAFVV+K GS LS   V++YVA+QV
Sbjct: 494 EDEEAGEIPVAFVVRKSGSTLSCTHVMEYVAKQV 522

BLAST of Bhi05G000002 vs. Swiss-Prot
Match: sp|Q84P21|4CLL5_ARATH (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 449.1 bits (1154), Expect = 7.2e-125
Identity = 237/548 (43.25%), Postives = 358/548 (65.33%), Query Frame = 0

Query: 26  RSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFLDVVSFIFSFKHNGNSAIIDSSTGSSI 85
           RS   N    +YS  T I         +P +P LDV +FI S  H G  A ID+STG ++
Sbjct: 7   RSGFCNSNSTFYSKRTPI--------PLPPNPSLDVTTFISSQAHRGRIAFIDASTGQNL 66

Query: 86  SYKEMFHMVKSMASGLQKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSS 145
           ++ E++  V+S+A  L + GI +G VVLL+ PNSI +P++ L V+ LGA++TT  P ++S
Sbjct: 67  TFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTS 126

Query: 146 SEIKKRINDCNVRLAFTITQKIKNF----ESLGIQAIGVPEITNFDLMQPMGFSYFHELI 205
           +EI K+I D N  LAFT +Q +       + L I  +    + +   ++ +      E  
Sbjct: 127 NEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKE-- 186

Query: 206 SSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYLPT 265
            SG+ +K+R  + Q+DTA LL+SSGTTG+SKGV+ +HRN I+ ++  V    S       
Sbjct: 187 PSGNRVKER--VDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSD----DG 246

Query: 266 ENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIM 325
           E  ++  +PMFHIYGL+ F  GLL+ GS+++++SKF++ E++ AI +++ T  P+VPPI+
Sbjct: 247 EQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPIL 306

Query: 326 TAMARTAEKI-GVHRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGT 385
            AM   A++I   +   S+  V CG A L+K+  + F + +P V  +QGYG+TEST IG 
Sbjct: 307 VAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGA 366

Query: 386 RGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEAT 445
                + +R Y + G L+ + E ++VD V+G  + P +TGEL L+GP  MKGY +N EAT
Sbjct: 367 STDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEAT 426

Query: 446 MSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTA 505
            ST+D E WL TGD+ Y D DG+++V+DRLKE+IKYKG+Q+AP +LEA+++THPE+ D A
Sbjct: 427 SSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAA 486

Query: 506 VTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGK 565
           V    D+E G+ P+A+VV+K GS LS+K ++++VA+QVAPYK++RKV F  SIPK+ +GK
Sbjct: 487 VIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGK 538

Query: 566 VLRRELGK 569
           +LR++L K
Sbjct: 547 ILRKDLIK 538

BLAST of Bhi05G000002 vs. Swiss-Prot
Match: sp|Q10S72|4CLL4_ORYSJ (4-coumarate--CoA ligase-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL4 PE=2 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 7.5e-122
Identity = 235/545 (43.12%), Postives = 354/545 (64.95%), Query Frame = 0

Query: 37  YSPDTGICRSVHASRGVPSDPFLDVVSFIFSFKHNGNSAIIDSSTGSSISYKEMFHMVKS 96
           Y   T   RS  A   +P+DP +DVVSF+ S +H+G  A++D++TG  I++ E++  V  
Sbjct: 15  YCAATRTFRSRRADVPLPADPEVDVVSFLASRRHSGVVALVDAATGRRITFTELWRAVAG 74

Query: 97  MASGLQKFGIS--QGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKRIND 156
            AS L    +S  +G V L++ PNS+ +P+  L  + LGAV+TT  P ++ +EI K++ D
Sbjct: 75  AASALAAHPVSLRKGHVALILSPNSVHFPVAALAAMSLGAVLTTANPLNTPAEIAKQVAD 134

Query: 157 CNVRLAFT---ITQKIKNFESLGIQAIGVPEITNFDLMQPMGFSYFHELISSGSD-LKQR 216
               LAFT   +  K+     L +  +    +   D   P   +   E+ ++  D  +++
Sbjct: 135 ARPVLAFTTRELLPKLPRAHDLRVVLLESARLPG-DSSDPRIVATIEEISATTPDPARRK 194

Query: 217 PVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFV-RFEASQYEYLPTENVYLAAI 276
             + Q+D A LL+SSGTTG SKGV+ THR+ IS +++ + RF     +   T   +L  +
Sbjct: 195 DRVTQDDPATLLYSSGTTGPSKGVVATHRSLISMVQIIMTRFRLEGSDKTET---FLCTV 254

Query: 277 PMFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIMTAMARTAE 336
           PMFH+YGL  F  GLL  G++VV++SK+++ E++++I+ + VT+ P+VPPI+ AM    +
Sbjct: 255 PMFHVYGLVAFATGLLGCGATVVVLSKYELPEMLRSINAYGVTYLPLVPPILVAMVAHPK 314

Query: 337 KIGVHRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGTRGFNTKNAR 396
            + + + R   +V  G A L K+ I+ F + +P V+ +QGYG+TESTAIG    + + +R
Sbjct: 315 PLPLGQMR---KVLSGGAPLGKELIEGFREKYPQVEILQGYGLTESTAIGASTDSAEESR 374

Query: 397 NYTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDKENW 456
            Y + GLL+PNTEAK+VD  SG  +P  +TGEL +RGP  MKGY  N EAT ST+  + W
Sbjct: 375 RYGTAGLLSPNTEAKIVDPDSGEALPVNRTGELWIRGPYVMKGYFKNAEATQSTLTPDGW 434

Query: 457 LHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTAVTAAKDEEC 516
           L TGD+ Y D DGYL+V+DRLKE+IKYKG+Q+ P +LEA+++THPEV D AV    D E 
Sbjct: 435 LKTGDLCYIDEDGYLFVVDRLKELIKYKGYQVPPAELEALLLTHPEVTDVAVIPFPDREV 494

Query: 517 GEIPVAFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGKVLRRELGKH 575
           G+ P+A++V+K GS LS+++V+++VA+QVAPYKKVRKV F   IPK+A+GK+LR++L K 
Sbjct: 495 GQFPMAYIVRKKGSNLSEREVMEFVAKQVAPYKKVRKVAFVTDIPKNASGKILRKDLIKL 552

BLAST of Bhi05G000002 vs. TAIR10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 644.8 bits (1662), Expect = 5.0e-185
Identity = 318/552 (57.61%), Postives = 429/552 (77.72%), Query Frame = 0

Query: 25  PRSEQTNKYPN-WYSPDTGICRSVHASRGVPSDPFLDVVSFIFSFKHNGNSAIIDSSTGS 84
           P+++ +++ P  W+S  TGI  S   S  +P DP LD VS +FS KH+G++A+IDS TG 
Sbjct: 12  PKTQTSHQNPPFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFSHKHHGDTALIDSLTGF 71

Query: 85  SISYKEMFHMVKSMASGL-QKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQ 144
           SIS+ E+  MV+SMA+G+    G+ QGDVV LVLPNS+++P+I L ++ LGA+VTTM P 
Sbjct: 72  SISHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPS 131

Query: 145 SSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQAIGVPEITNFDLMQPMGFSYFHELIS 204
           SS  EIKK++++C+V LAFT T+ ++   SLG+  I V E  +FD ++ +    F+ ++ 
Sbjct: 132 SSLGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIR-IENPKFYSIMK 191

Query: 205 SGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYLPTE 264
                  +P+I+Q+D AA+++SSGTTG SKGV+LTHRN I+++ELFVRFEASQYEY  + 
Sbjct: 192 ESFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSS 251

Query: 265 NVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIMT 324
           NVYLAA+P+ HIYGLS+FVMGLLSLGS++V+M +FD  +VV  I+RFK+THFPVVPP++ 
Sbjct: 252 NVYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLM 311

Query: 325 AMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGTRG 384
           A+ + A+ +    F+SLKQVS GAA L++K I+DF++  PHVD IQGYGMTESTA+GTRG
Sbjct: 312 ALTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRG 371

Query: 385 FNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEATMS 444
           FN++    Y+SVGLLAPN +AKVVDW SGSF+PPG  GEL ++GPG MKGYLNNP+AT  
Sbjct: 372 FNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQM 431

Query: 445 TIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTAVT 504
           +I +++WL TGDI YFD DGYL+++DR+KE+IKYKGFQIAP DLEAV+++HP ++D AVT
Sbjct: 432 SIVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVT 491

Query: 505 AAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGKVL 564
           AA +EECGEIPVAFVV++  + LS++DVI YVA QVAPY+KVRKVV   SIPKS  GK+L
Sbjct: 492 AAPNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKIL 551

Query: 565 RRELGKHLPSKL 575
           R+EL + L + +
Sbjct: 552 RKELKRILTNSV 562

BLAST of Bhi05G000002 vs. TAIR10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1)

HSP 1 Score: 449.1 bits (1154), Expect = 4.0e-126
Identity = 237/548 (43.25%), Postives = 358/548 (65.33%), Query Frame = 0

Query: 26  RSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFLDVVSFIFSFKHNGNSAIIDSSTGSSI 85
           RS   N    +YS  T I         +P +P LDV +FI S  H G  A ID+STG ++
Sbjct: 7   RSGFCNSNSTFYSKRTPI--------PLPPNPSLDVTTFISSQAHRGRIAFIDASTGQNL 66

Query: 86  SYKEMFHMVKSMASGLQKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSS 145
           ++ E++  V+S+A  L + GI +G VVLL+ PNSI +P++ L V+ LGA++TT  P ++S
Sbjct: 67  TFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTS 126

Query: 146 SEIKKRINDCNVRLAFTITQKIKNF----ESLGIQAIGVPEITNFDLMQPMGFSYFHELI 205
           +EI K+I D N  LAFT +Q +       + L I  +    + +   ++ +      E  
Sbjct: 127 NEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKE-- 186

Query: 206 SSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYLPT 265
            SG+ +K+R  + Q+DTA LL+SSGTTG+SKGV+ +HRN I+ ++  V    S       
Sbjct: 187 PSGNRVKER--VDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSD----DG 246

Query: 266 ENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIM 325
           E  ++  +PMFHIYGL+ F  GLL+ GS+++++SKF++ E++ AI +++ T  P+VPPI+
Sbjct: 247 EQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPIL 306

Query: 326 TAMARTAEKI-GVHRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGT 385
            AM   A++I   +   S+  V CG A L+K+  + F + +P V  +QGYG+TEST IG 
Sbjct: 307 VAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGA 366

Query: 386 RGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEAT 445
                + +R Y + G L+ + E ++VD V+G  + P +TGEL L+GP  MKGY +N EAT
Sbjct: 367 STDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEAT 426

Query: 446 MSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTA 505
            ST+D E WL TGD+ Y D DG+++V+DRLKE+IKYKG+Q+AP +LEA+++THPE+ D A
Sbjct: 427 SSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAA 486

Query: 506 VTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGK 565
           V    D+E G+ P+A+VV+K GS LS+K ++++VA+QVAPYK++RKV F  SIPK+ +GK
Sbjct: 487 VIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGK 538

Query: 566 VLRRELGK 569
           +LR++L K
Sbjct: 547 ILRKDLIK 538

BLAST of Bhi05G000002 vs. TAIR10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 420.6 bits (1080), Expect = 1.5e-117
Identity = 231/549 (42.08%), Postives = 341/549 (62.11%), Query Frame = 0

Query: 37  YSPDTGICRSVHASRGVPSDPFLDVVSFIFSFKHNGNSAIIDSSTGSSISYKEMFHMVKS 96
           +   T I  S      +P + FLDV SFI S  H G +  +D+ TG  +S+ E++  V+ 
Sbjct: 27  FCESTSIFYSKREPMALPPNQFLDVTSFIASQPHRGKTVFVDAVTGRRLSFPELWLGVER 86

Query: 97  MASGLQKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCN 156
           +A  L   G+ +G+VV+++ PNSI +PI+ L V+ LGA++TT  P ++S EI K+I D  
Sbjct: 87  VAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEISKQIGDSR 146

Query: 157 VRLAFTITQKIK--------NFESLGIQAIGVPEITNFDLMQPMG-FSYFHELISSGSDL 216
             LAFT  + +         N   + +    VP  +  D ++ +G      E   S S +
Sbjct: 147 PVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIETEPSESRV 206

Query: 217 KQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELF-VRFEASQYEYLPTENVYL 276
           KQR  + Q+DTAALL+SSGTTG SKGVML+HRN I+ ++ +  RF   Q          +
Sbjct: 207 KQR--VNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRARFGLEQRT--------I 266

Query: 277 AAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIMTAMAR 336
             IPM HI+G   F  GL++LG ++V++ KFD+ +++ A++  + ++  +VPPI+ AM  
Sbjct: 267 CTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMVN 326

Query: 337 TAEKI-GVHRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGTRGFNT 396
            A +I   +   SL  V  G A L+++  + FV+ +P V  +QGYG+TESTAI    FN 
Sbjct: 327 GANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFNK 386

Query: 397 KNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEATMSTID 456
           +  + Y + GLLAPN E K+VD  +G  +   +TGEL +R P  MKGY  N EAT STID
Sbjct: 387 EETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTID 446

Query: 457 KENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTAVTAAK 516
            E WL TGD+ Y D DG+++V+DRLKE+IK  G+Q+AP +LEA+++ HPE+ D AV    
Sbjct: 447 SEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPIP 506

Query: 517 DEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGKVLRRE 575
           D + G+ P+A++V+K GS LS+ +++ +VA+QV+PYKK+RKV F  SIPK+ +GK+LRRE
Sbjct: 507 DMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILRRE 565

BLAST of Bhi05G000002 vs. TAIR10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 416.8 bits (1070), Expect = 2.2e-116
Identity = 223/540 (41.30%), Postives = 338/540 (62.59%), Query Frame = 0

Query: 42  GICRSVHASRGVPSDPFLDVVSFIF--SFKHNGNSAIIDSSTGSSISYKEMFHMVKSMAS 101
           GI RS+  +  +P DP   +VSF+F  S  +    AI DS TG S+++ ++   V  +A 
Sbjct: 10  GIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAH 69

Query: 102 GLQKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRL 161
           G  + GI + DVVL+  PNS  +P+  L V  +G V TT  P  + +E+ K+I D N ++
Sbjct: 70  GFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKI 129

Query: 162 AFTITQ---KIKNFESLGIQAIGVPEITNFDLMQPMGFSYFHELISSGSDLKQRPV--IR 221
             ++ Q   KIK F+ L +  +G  +              F  ++     + + P   I+
Sbjct: 130 IISVNQLFDKIKGFD-LPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVSEYPFVEIK 189

Query: 222 QEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYLPTENVYLAAIPMFHI 281
           Q DTAALL+SSGTTG SKGV LTH NFI+   +    +    EY     V+L  +PMFH+
Sbjct: 190 QSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEY---HGVFLCFLPMFHV 249

Query: 282 YGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVH 341
           +GL++     L  G+++V M++F+++ V+K I++F+VTH  VVPP+  A+++ +  +   
Sbjct: 250 FGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQS-IVKKF 309

Query: 342 RFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSV 401
              SLK +  GAA L K  +++  +  P+V  +QGYGMTE+  I +        RN  S 
Sbjct: 310 DLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGKRNSGSA 369

Query: 402 GLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGD 461
           G+LAP  EA++V   +G   PP + GE+ +RGP  MKGYLNNP+AT  TIDK++W+HTGD
Sbjct: 370 GMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGD 429

Query: 462 IVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPV 521
           + YF+ DG LYV+DR+KE+IKYKGFQ+AP +LE ++++HP++LD  V    DEE GE+P+
Sbjct: 430 LGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPI 489

Query: 522 AFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGKVLRRELGKHLPSKL 575
           AFVV+ P S ++++D+  ++A+QVAPYK++R+V F   +PKSAAGK+LRREL + + SK+
Sbjct: 490 AFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQQVRSKM 544

BLAST of Bhi05G000002 vs. TAIR10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 410.2 bits (1053), Expect = 2.1e-114
Identity = 225/548 (41.06%), Postives = 345/548 (62.96%), Query Frame = 0

Query: 39  PDTGICRS---VHASR---GVPSDPFLDVVSFIFSFKHNGNSAIIDSSTGSSISYKEMFH 98
           P +G C+S    ++ R    +P +   DV +FI S  H G +A ID++TG  +++ +++ 
Sbjct: 12  PRSGFCKSNSTFYSKRQPLSLPPNLSRDVTTFISSQPHRGKTAFIDAATGQCLTFSDLWR 71

Query: 99  MVKSMASGL-QKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKR 158
            V  +A  L  + GI +GDVVL++ PNSIF P++ L V+ LGAV TT    ++S EI K+
Sbjct: 72  AVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEISKQ 131

Query: 159 INDCNVRLAFTITQKIKNFESLGIQAIGVPEITNFDLMQPMG-FSYFHELIS---SGSDL 218
           I D N  L FT T+++     + I  +   +    +L   +       E++    SG  +
Sbjct: 132 IADSNPTLVFT-TRQLAPKLPVAISVVLTDDEVYQELTSAIRVVGILSEMVKKEPSGQRV 191

Query: 219 KQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYLPTENVYLA 278
           + R  + Q+DTA +L+SSGTTG SKGV+ +HRN  + +  F+       + L  +++++ 
Sbjct: 192 RDR--VNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFIS------DNLKRDDIFIC 251

Query: 279 AIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIMTAMART 338
            +PMFH YGL  F MG ++LGS+VVI+ +F + +++ A+++ + T   + PP++ AM   
Sbjct: 252 TVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVLVAMIND 311

Query: 339 AEKI-GVHRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGTRGFNTK 398
           A+ I   +   SLK V CG A L+K+  + F++ +P VD +QGY +TES   G    + +
Sbjct: 312 ADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGGAFTNSAE 371

Query: 399 NARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDK 458
            +R Y + G L  + EA++VD  +G FM   +TGEL L+GP   KGY  N EAT  TI+ 
Sbjct: 372 ESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEATNETINL 431

Query: 459 ENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTAVTAAKD 518
           E WL TGD+ Y D DG+L+V+DRLKE+IKYKG+Q+ P +LEA+++THP++LD AV    D
Sbjct: 432 EGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDAAVIPFPD 491

Query: 519 EECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGKVLRREL 575
           +E G+ P+A+VV+K  S LS+K VID++++QVAPYKK+RKV F  SIPK+A+GK LR++L
Sbjct: 492 KEAGQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTASGKTLRKDL 550

BLAST of Bhi05G000002 vs. TrEMBL
Match: tr|A0A0A0L8X1|A0A0A0L8X1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G638510 PE=4 SV=1)

HSP 1 Score: 1000.0 bits (2584), Expect = 2.2e-288
Identity = 499/574 (86.93%), Postives = 542/574 (94.43%), Query Frame = 0

Query: 1   MAPLLNDHFSYQTPENETVITKKIPRSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFLD 60
           MA L+N+ FS++TPENE VITKKIPRSE+TNKYPNWYSPDTGICRSVH  R +PSDPFLD
Sbjct: 1   MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD 60

Query: 61  VVSFIFS--FKHNGNSAIIDSSTGSSISYKEMFHMVKSMASGLQKFGISQGDVVLLVLPN 120
           VVSFIFS  F+HNG+SA+IDSSTG+SISYKE+F MVKSMASGL   GISQGDVVLL+LPN
Sbjct: 61  VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN 120

Query: 121 SIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQAI 180
           SIF+PII+LG LYLGAVVTTMFPQSSSSEIKKRI DCNVRLAF ITQKIKNFE+LGI+ I
Sbjct: 121 SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI 180

Query: 181 GVPEITNFDLMQPMGFSYFHELISSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTH 240
           GVPE TNF+LM+ MGFS F+ELIS GSDL ++PVIRQEDTAA+LFSSGTTGVSKGVML+H
Sbjct: 181 GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH 240

Query: 241 RNFISTIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFD 300
           RNFISTIELFVRFEASQYEYLPT+NVYLAA+PMFHIYGLSIFVMGL+SLGSSVV+MSKFD
Sbjct: 241 RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD 300

Query: 301 VKEVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDFV 360
           VK+VVKAIDRFKVTHFPVVPPI+TA+ARTAEKIGVHRFRSLKQVSCGAA+ +KKTID+FV
Sbjct: 301 VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV 360

Query: 361 KAFPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGK 420
            A PHVDFIQGYGMTESTA+GTRGFNTKNARNY SVGLLAPNTEAKVVDWV+GS MPPGK
Sbjct: 361 HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK 420

Query: 421 TGELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKG 480
           TGELLLRGPGSMKGYLNNPEAT  T+D+ENWLHTGDIVYFDRDGYLYV+DRLKEVIKYKG
Sbjct: 421 TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG 480

Query: 481 FQIAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQV 540
           FQIAPTDLEAVV+THPEVLDTAV AAKDEECGEIPVAFVVKKPGS L+QKDV+DYVAQQV
Sbjct: 481 FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV 540

Query: 541 APYKKVRKVVFTESIPKSAAGKVLRRELGKHLPS 573
           APYKKVRKV+FTESIPKSAAGKVLRREL KH P+
Sbjct: 541 APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPA 574

BLAST of Bhi05G000002 vs. TrEMBL
Match: tr|A0A1S3CCH7|A0A1S3CCH7_CUCME (4-coumarate--CoA ligase-like 6 OS=Cucumis melo OX=3656 GN=LOC103499264 PE=4 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 5.2e-269
Identity = 472/552 (85.51%), Postives = 510/552 (92.39%), Query Frame = 0

Query: 1   MAPLL-NDHFSYQTPENETVITKKIPRSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFL 60
           MA LL ND FS++TPENE  ITKKIP SE+TNKYPNWYSPDTGIC SVH  R +PSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFS--FKHNGNSAIIDSSTGSSISYKEMFHMVKSMASGLQKFGISQGDVVLLVLP 120
           DVVSFIFS  F+HNG+SA+IDS TG+SISYKE+F MVKSMASGL   GISQGDVVLL+LP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQA 180
           NSIFYPII+LG LYLGAV+TTMFPQSSSSEIKKRI DCNVRLAFTITQKIKNFE+LGI+ 
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPEITNFDLMQPMGFSYFHELISSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLT 240
           IGVPE TNFDLM+PMGFS F+ELISSGSDL +RPVIRQEDTAA+LFSSGTTGVSKGVML+
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKF 300
           HRNFISTIELFVRFEASQYEYLPT+NVYLAAIPMFHIYGLSIFVMGL+SLGSSVV+MSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKEVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDF 360
           DVK+VVKAIDRFKVTHFPVVPPI+ A++RTAEKIGV RFRSLKQVSCGAA+ +KKTIDDF
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VKAFPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPG 420
           V+A  HVDFIQGYGMTESTAIGTRGFN K  RNY SVGLLAPNTEAKVVDWV+GS MPPG
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEATMSTID+ENWLHTGDIVYFDRDGYLYV+DRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQ 540
           GFQIAPTDLEAVV+ HPEVLDTAV AAKDEECGEIPVAFVVKKPGS LSQKDV+DYVAQQ
Sbjct: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540

Query: 541 VAPYKKVRKVVF 550
           V+   ++   +F
Sbjct: 541 VSHIFQICPCIF 552

BLAST of Bhi05G000002 vs. TrEMBL
Match: tr|A0A2P5AZ24|A0A2P5AZ24_PARAD (4-coumarate-CoA ligase OS=Parasponia andersonii OX=3476 GN=PanOPCL2 PE=4 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 3.9e-216
Identity = 380/548 (69.34%), Postives = 462/548 (84.31%), Query Frame = 0

Query: 28  EQTNKYPNWYSPDTGICRSVHASRGVPSDPFLDVVSFIFSFKHNGNSAIIDSSTGSSISY 87
           E+ +++P+WYSP+TGIC S HASR +PSDPFLDVVSFIFS + NG SA+IDSSTGSS+SY
Sbjct: 15  EEVSQFPHWYSPETGICHSKHASRKLPSDPFLDVVSFIFSNQQNGVSALIDSSTGSSLSY 74

Query: 88  KEMFHMVKSMASGLQKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSSSE 147
            E+ H+V+SMASGL   GIS+ DVVL++LPNSI+YP+ILLGVLYLGA+VTTM P SS +E
Sbjct: 75  SELHHLVQSMASGLHSIGISKSDVVLVLLPNSIYYPVILLGVLYLGAIVTTMNPLSSVTE 134

Query: 148 IKKRINDCNVRLAFTITQKIKNFESLGIQAIGVPEITNFDL-MQPMGFSYFHELISSGSD 207
           IKK+++D    LAFTI +K++ F+ LG+  I VPE  NF+L +    FS F +L+S   D
Sbjct: 135 IKKQVSDSKACLAFTIPEKVEVFQVLGVTPIEVPE--NFNLGLNSSRFSAFSKLVSGYID 194

Query: 208 LKQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYLPTENVYL 267
           L  R +I+Q+D AA+++SSGTTGVSKGV+LTHRNFI+ +ELFVRFEASQYEY   +NVYL
Sbjct: 195 LVPRSLIKQQDAAAIMYSSGTTGVSKGVVLTHRNFIAMVELFVRFEASQYEYSSLKNVYL 254

Query: 268 AAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIMTAMAR 327
           A +PMFHIYGLS+FVMGLLSLGS++V+M +F++ EVVKAIDRF VTHFPVVPPI+TA+ R
Sbjct: 255 ATLPMFHIYGLSLFVMGLLSLGSTIVVMRRFNINEVVKAIDRFHVTHFPVVPPILTALTR 314

Query: 328 TAEKIGVHRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGTRGFNTK 387
           TA+ +    FRSLKQVS GAA L+KK I+DFV+ FP VDFIQGYGMTESTA+GTRGFNTK
Sbjct: 315 TAKDLHGRSFRSLKQVSSGAAPLSKKIIEDFVETFPDVDFIQGYGMTESTAVGTRGFNTK 374

Query: 388 NARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDK 447
             +N++SVGLLAPN +AKVVDW +G+F+PPG +GEL LRGP  MKGYLNN  ATMSTIDK
Sbjct: 375 KFQNFSSVGLLAPNMQAKVVDWTTGAFLPPGCSGELWLRGPAVMKGYLNNAVATMSTIDK 434

Query: 448 ENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTAVTAAKD 507
           + WLHTGDIV+FD+DG+L++IDRLKE+IKYKGFQIAP DLEAV+++HPE+ D AV A KD
Sbjct: 435 DGWLHTGDIVFFDQDGFLHIIDRLKEIIKYKGFQIAPADLEAVLISHPEIQDVAVAAVKD 494

Query: 508 EECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGKVLRREL 567
           E  GEIPVAFVVKK GS LSQ+ V+DYVA QVAPYKK+RKVVFT SIPKSAAGK+LRREL
Sbjct: 495 ENSGEIPVAFVVKKHGSSLSQEAVMDYVAGQVAPYKKIRKVVFTHSIPKSAAGKILRREL 554

Query: 568 GKHLPSKL 575
           GK L S+L
Sbjct: 555 GKLLISRL 560

BLAST of Bhi05G000002 vs. TrEMBL
Match: tr|A0A2P5FAB0|A0A2P5FAB0_9ROSA (4-coumarate-CoA ligase OS=Trema orientalis OX=63057 GN=TorOPCL2 PE=4 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 5.2e-213
Identity = 373/547 (68.19%), Postives = 454/547 (83.00%), Query Frame = 0

Query: 28  EQTNKYPNWYSPDTGICRSVHASRGVPSDPFLDVVSFIFSFKHNGNSAIIDSSTGSSISY 87
           E+ +++P+WYSP+TGIC S H  R +PSDPFLDVVSFIFS +HNG SA+IDSSTGSS+SY
Sbjct: 15  EEVSQFPHWYSPETGICHSRHGPRNLPSDPFLDVVSFIFSNQHNGVSALIDSSTGSSLSY 74

Query: 88  KEMFHMVKSMASGLQKFGISQGDVVLLVLPNSIFYPIILLGVLYLGAVVTTMFPQSSSSE 147
            E+ H+V+SMASGL   GIS+ DVVL++LPNSI+YP+ILL VLYLGA+VTTM P SS +E
Sbjct: 75  SELHHLVQSMASGLHSIGISKSDVVLVLLPNSIYYPVILLAVLYLGAIVTTMNPLSSVTE 134

Query: 148 IKKRINDCNVRLAFTITQKIKNFESLGIQAIGVPEITNFDLMQPMGFSYFHELISSGSDL 207
           IKK+++D    LAFT+ +K++ F+ LG+  I VPE  N DL     FS F +L+S   DL
Sbjct: 135 IKKQVSDSKACLAFTVPEKVEVFQVLGVTPIEVPENFNLDL-NSSRFSAFSKLVSGDIDL 194

Query: 208 KQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRNFISTIELFVRFEASQYEYLPTENVYLA 267
             R +I+Q+D AA+++SSGTTGVSKGV+LTHRNFI+ +ELFVRFEASQYEY   +NVYLA
Sbjct: 195 VPRSLIKQQDAAAIMYSSGTTGVSKGVVLTHRNFIAMVELFVRFEASQYEYSSFKNVYLA 254

Query: 268 AIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVKEVVKAIDRFKVTHFPVVPPIMTAMART 327
            +PMFHIYGLS+FVMGL SLGS++V+M +       KAI+RF+VTHFPVVPPI+TA+ RT
Sbjct: 255 TLPMFHIYGLSLFVMGLFSLGSTIVVMRRXXXXXXXKAIERFQVTHFPVVPPILTALTRT 314

Query: 328 AEKIGVHRFRSLKQVSCGAASLNKKTIDDFVKAFPHVDFIQGYGMTESTAIGTRGFNTKN 387
           A+ +    FRSLKQVS GAA L+KK I+DFV+ FP VDFIQGYGMTESTAIGTRGFNTK 
Sbjct: 315 AKDLHGRSFRSLKQVSSGAAPLSKKIIEDFVETFPDVDFIQGYGMTESTAIGTRGFNTKK 374

Query: 388 ARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTGELLLRGPGSMKGYLNNPEATMSTIDKE 447
            +N++SVGLLAPN +AKVVDW +G+F+PPG +GEL LRGP  MKGYLNN  ATMSTIDK+
Sbjct: 375 FQNFSSVGLLAPNMQAKVVDWTTGAFLPPGCSGELWLRGPAVMKGYLNNAVATMSTIDKD 434

Query: 448 NWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDTAVTAAKDE 507
            WLHTGDIVYFD+DG+L++IDRLKE+IKYKGFQIAP DLEAV+++HPE+ D AV AAKDE
Sbjct: 435 GWLHTGDIVYFDQDGFLHIIDRLKEIIKYKGFQIAPADLEAVLISHPEIQDVAVAAAKDE 494

Query: 508 ECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAPYKKVRKVVFTESIPKSAAGKVLRRELG 567
           + GE+PVAFVVKK GS LSQ+ V+DYVA QVAPYKK+RKVVFT SIPKSAAGK+LRRELG
Sbjct: 495 DSGEMPVAFVVKKHGSSLSQEAVMDYVAGQVAPYKKIRKVVFTHSIPKSAAGKILRRELG 554

Query: 568 KHLPSKL 575
           K L S+L
Sbjct: 555 KLLTSRL 560

BLAST of Bhi05G000002 vs. TrEMBL
Match: tr|A0A2I4GJ31|A0A2I4GJ31_9ROSI (4-coumarate--CoA ligase-like 6 isoform X1 OS=Juglans regia OX=51240 GN=LOC109008312 PE=4 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 2.3e-208
Identity = 367/574 (63.94%), Postives = 452/574 (78.75%), Query Frame = 0

Query: 1   MAPLLNDHFSYQTPENETVITKKIPRSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFLD 60
           MA    + F+    EN T   +    ++    +P+WYSP+TGI  S H    +PSDPFLD
Sbjct: 1   MASTSTNLFNCHIKENNTTQIQNTSTTQNPKPHPDWYSPETGIYHSKHTPVELPSDPFLD 60

Query: 61  VVSFIFSFKHNGNSAIIDSSTGSSISYKEMFHMVKSMASGLQKFGISQGDVVLLVLPNSI 120
           VVSF+FS KH+G SA+ID S+GSS+SY E++ +V+SMASGL   G+S GDVVL++LPNSI
Sbjct: 61  VVSFLFSHKHHGVSALIDPSSGSSVSYPELYPLVESMASGLHIMGVSPGDVVLILLPNSI 120

Query: 121 FYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQAIGV 180
           +YPI+ LGVLYLGA+VTTM P S   EIKK+I+DCNV LAFT  +K+K  ++LGI AI V
Sbjct: 121 YYPILFLGVLYLGAIVTTMNPLSKVLEIKKQIDDCNVCLAFTSHEKVKKLQALGIPAIAV 180

Query: 181 PEITNFDLMQPMGFSYFHELISSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRN 240
           PE  N D  Q   FS F +LIS    L  RPVI+Q+DTAA+++SSGTTG+SKGV+LTH N
Sbjct: 181 PEDVNLDSKQ-TAFSAFFKLISGSFGLAPRPVIKQQDTAAIMYSSGTTGMSKGVVLTHGN 240

Query: 241 FISTIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVK 300
           FI+ +ELFVRFEASQYEY   +NV+LA +PMFHIYGLS+FV+GLL+LGSS+V+M KFD+ 
Sbjct: 241 FIAMLELFVRFEASQYEYSSVDNVFLAVLPMFHIYGLSLFVLGLLALGSSIVVMRKFDMN 300

Query: 301 EVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDFVKA 360
           EV+  IDR+ VTHFPVVPP+++A+ R A+       +SLKQVSCGAA L  K I+DF++ 
Sbjct: 301 EVMNVIDRYSVTHFPVVPPMLSALTRKAKDAVRSSCKSLKQVSCGAAPLTSKVIEDFIQT 360

Query: 361 FPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTG 420
            P VD IQGYGMTESTA+GTRGFNT+N +NY+SVGLLAPN +AKVVDW +GSF+PPG  G
Sbjct: 361 LPDVDLIQGYGMTESTAVGTRGFNTENIQNYSSVGLLAPNMQAKVVDWSTGSFLPPGSGG 420

Query: 421 ELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQ 480
           EL LRGP  MKGYLNN EATMST+D + WLHTGDIV FD++GY+++ DRLKE+IKYKGFQ
Sbjct: 421 ELWLRGPAIMKGYLNNAEATMSTVDTDGWLHTGDIVCFDQEGYIHIFDRLKEIIKYKGFQ 480

Query: 481 IAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAP 540
           IAP DLEAV+++HPE+LD AVT   DEE GEIPVAFVV+K GS+LS   V+DYV++QVAP
Sbjct: 481 IAPADLEAVLISHPEILDVAVTGTVDEEYGEIPVAFVVRKHGSRLSSAGVMDYVSEQVAP 540

Query: 541 YKKVRKVVFTESIPKSAAGKVLRRELGKHLPSKL 575
           YKKVRKVVFT SIPKSAAGK+LRREL   L SKL
Sbjct: 541 YKKVRKVVFTNSIPKSAAGKILRRELRILLTSKL 573

BLAST of Bhi05G000002 vs. NCBI nr
Match: XP_004144410.1 (PREDICTED: 4-coumarate--CoA ligase-like 6 [Cucumis sativus] >KGN58405.1 hypothetical protein Csa_3G638510 [Cucumis sativus])

HSP 1 Score: 1000.0 bits (2584), Expect = 3.4e-288
Identity = 499/574 (86.93%), Postives = 542/574 (94.43%), Query Frame = 0

Query: 1   MAPLLNDHFSYQTPENETVITKKIPRSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFLD 60
           MA L+N+ FS++TPENE VITKKIPRSE+TNKYPNWYSPDTGICRSVH  R +PSDPFLD
Sbjct: 1   MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD 60

Query: 61  VVSFIFS--FKHNGNSAIIDSSTGSSISYKEMFHMVKSMASGLQKFGISQGDVVLLVLPN 120
           VVSFIFS  F+HNG+SA+IDSSTG+SISYKE+F MVKSMASGL   GISQGDVVLL+LPN
Sbjct: 61  VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN 120

Query: 121 SIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQAI 180
           SIF+PII+LG LYLGAVVTTMFPQSSSSEIKKRI DCNVRLAF ITQKIKNFE+LGI+ I
Sbjct: 121 SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI 180

Query: 181 GVPEITNFDLMQPMGFSYFHELISSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTH 240
           GVPE TNF+LM+ MGFS F+ELIS GSDL ++PVIRQEDTAA+LFSSGTTGVSKGVML+H
Sbjct: 181 GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH 240

Query: 241 RNFISTIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFD 300
           RNFISTIELFVRFEASQYEYLPT+NVYLAA+PMFHIYGLSIFVMGL+SLGSSVV+MSKFD
Sbjct: 241 RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD 300

Query: 301 VKEVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDFV 360
           VK+VVKAIDRFKVTHFPVVPPI+TA+ARTAEKIGVHRFRSLKQVSCGAA+ +KKTID+FV
Sbjct: 301 VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV 360

Query: 361 KAFPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGK 420
            A PHVDFIQGYGMTESTA+GTRGFNTKNARNY SVGLLAPNTEAKVVDWV+GS MPPGK
Sbjct: 361 HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK 420

Query: 421 TGELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKG 480
           TGELLLRGPGSMKGYLNNPEAT  T+D+ENWLHTGDIVYFDRDGYLYV+DRLKEVIKYKG
Sbjct: 421 TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG 480

Query: 481 FQIAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQV 540
           FQIAPTDLEAVV+THPEVLDTAV AAKDEECGEIPVAFVVKKPGS L+QKDV+DYVAQQV
Sbjct: 481 FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV 540

Query: 541 APYKKVRKVVFTESIPKSAAGKVLRRELGKHLPS 573
           APYKKVRKV+FTESIPKSAAGKVLRREL KH P+
Sbjct: 541 APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPA 574

BLAST of Bhi05G000002 vs. NCBI nr
Match: XP_023554003.1 (4-coumarate--CoA ligase-like 6 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 979.9 bits (2532), Expect = 3.6e-282
Identity = 487/574 (84.84%), Postives = 529/574 (92.16%), Query Frame = 0

Query: 1   MAPLLNDHFSYQTPENETVITKKIPRSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFLD 60
           MAPLLND FS+QTPE++TV TKKIP     NKYP WYSPDTGICRSVHASR +P+DPFLD
Sbjct: 5   MAPLLNDQFSFQTPESQTVQTKKIP-----NKYPGWYSPDTGICRSVHASRDLPTDPFLD 64

Query: 61  VVSFIFSFKHNGNSAIIDSSTGSSISYKEMFHMVKSMASGLQKFGISQGDVVLLVLPNSI 120
           V S+I SF+HNG SA+IDSSTG SISY+E++ MV SMASGLQK G+SQGDVVLL+LPNSI
Sbjct: 65  VASYILSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSI 124

Query: 121 FYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQAIGV 180
           FYPI+LLGVLYLGAV+TTMFPQSSS EIKKRI++CNVRLAF   Q + NFE+LG+QAIGV
Sbjct: 125 FYPIVLLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGV 184

Query: 181 PEITNFDLMQPMGFSYFHELISSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRN 240
           PE TN DLM+PMGFS F+ELIS G DL +RPVIRQEDTAA+LFSSGTTGVSKGV+LTHRN
Sbjct: 185 PENTNLDLMRPMGFSSFYELISGGLDLNKRPVIRQEDTAAILFSSGTTGVSKGVLLTHRN 244

Query: 241 FISTIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVK 300
           FIST+ELFVRFEASQYEYL TENVYLAAIPMFH+YGLSIFVMGLLSLGSS+V+MSKFDVK
Sbjct: 245 FISTVELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVK 304

Query: 301 EVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDFVKA 360
           EVV AIDRFKVTHFPVVPPIM  MARTA K G H+FRSLKQVSCGAASL+KK I DFV+A
Sbjct: 305 EVVMAIDRFKVTHFPVVPPIMILMARTAGKFGGHKFRSLKQVSCGAASLSKKIIGDFVQA 364

Query: 361 FPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTG 420
            PHVDFIQGYGMTE+TA+GTRGFNTK A+NY SVGLLAPN EAKVVDWVSGS MPPGKTG
Sbjct: 365 LPHVDFIQGYGMTETTAVGTRGFNTKKAQNYLSVGLLAPNMEAKVVDWVSGSIMPPGKTG 424

Query: 421 ELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQ 480
           ELLLRGPG MKGYLNNPEATMSTID+ENWLHTGDIVYFDRDGYLYV+DRLKEVIKYKGFQ
Sbjct: 425 ELLLRGPGLMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQ 484

Query: 481 IAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAP 540
           IAPTDLE+VV+THPEVLDTAV AA+DEECGEIPVAFVVKKPGS LSQKDVIDYVAQQVAP
Sbjct: 485 IAPTDLESVVITHPEVLDTAVAAAEDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQQVAP 544

Query: 541 YKKVRKVVFTESIPKSAAGKVLRRELGKHLPSKL 575
           YKK+RKVVFTESIPKSAAGKVLR+ELGKHLPSKL
Sbjct: 545 YKKIRKVVFTESIPKSAAGKVLRKELGKHLPSKL 573

BLAST of Bhi05G000002 vs. NCBI nr
Match: XP_023554005.1 (4-coumarate--CoA ligase-like 6 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023554006.1 4-coumarate--CoA ligase-like 6 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 979.9 bits (2532), Expect = 3.6e-282
Identity = 487/574 (84.84%), Postives = 529/574 (92.16%), Query Frame = 0

Query: 1   MAPLLNDHFSYQTPENETVITKKIPRSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFLD 60
           MAPLLND FS+QTPE++TV TKKIP     NKYP WYSPDTGICRSVHASR +P+DPFLD
Sbjct: 1   MAPLLNDQFSFQTPESQTVQTKKIP-----NKYPGWYSPDTGICRSVHASRDLPTDPFLD 60

Query: 61  VVSFIFSFKHNGNSAIIDSSTGSSISYKEMFHMVKSMASGLQKFGISQGDVVLLVLPNSI 120
           V S+I SF+HNG SA+IDSSTG SISY+E++ MV SMASGLQK G+SQGDVVLL+LPNSI
Sbjct: 61  VASYILSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSI 120

Query: 121 FYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQAIGV 180
           FYPI+LLGVLYLGAV+TTMFPQSSS EIKKRI++CNVRLAF   Q + NFE+LG+QAIGV
Sbjct: 121 FYPIVLLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGV 180

Query: 181 PEITNFDLMQPMGFSYFHELISSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRN 240
           PE TN DLM+PMGFS F+ELIS G DL +RPVIRQEDTAA+LFSSGTTGVSKGV+LTHRN
Sbjct: 181 PENTNLDLMRPMGFSSFYELISGGLDLNKRPVIRQEDTAAILFSSGTTGVSKGVLLTHRN 240

Query: 241 FISTIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVK 300
           FIST+ELFVRFEASQYEYL TENVYLAAIPMFH+YGLSIFVMGLLSLGSS+V+MSKFDVK
Sbjct: 241 FISTVELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVK 300

Query: 301 EVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDFVKA 360
           EVV AIDRFKVTHFPVVPPIM  MARTA K G H+FRSLKQVSCGAASL+KK I DFV+A
Sbjct: 301 EVVMAIDRFKVTHFPVVPPIMILMARTAGKFGGHKFRSLKQVSCGAASLSKKIIGDFVQA 360

Query: 361 FPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTG 420
            PHVDFIQGYGMTE+TA+GTRGFNTK A+NY SVGLLAPN EAKVVDWVSGS MPPGKTG
Sbjct: 361 LPHVDFIQGYGMTETTAVGTRGFNTKKAQNYLSVGLLAPNMEAKVVDWVSGSIMPPGKTG 420

Query: 421 ELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQ 480
           ELLLRGPG MKGYLNNPEATMSTID+ENWLHTGDIVYFDRDGYLYV+DRLKEVIKYKGFQ
Sbjct: 421 ELLLRGPGLMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQ 480

Query: 481 IAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAP 540
           IAPTDLE+VV+THPEVLDTAV AA+DEECGEIPVAFVVKKPGS LSQKDVIDYVAQQVAP
Sbjct: 481 IAPTDLESVVITHPEVLDTAVAAAEDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQQVAP 540

Query: 541 YKKVRKVVFTESIPKSAAGKVLRRELGKHLPSKL 575
           YKK+RKVVFTESIPKSAAGKVLR+ELGKHLPSKL
Sbjct: 541 YKKIRKVVFTESIPKSAAGKVLRKELGKHLPSKL 569

BLAST of Bhi05G000002 vs. NCBI nr
Match: XP_022963967.1 (4-coumarate--CoA ligase-like 6 isoform X2 [Cucurbita moschata] >XP_022963968.1 4-coumarate--CoA ligase-like 6 isoform X2 [Cucurbita moschata])

HSP 1 Score: 978.4 bits (2528), Expect = 1.0e-281
Identity = 486/574 (84.67%), Postives = 528/574 (91.99%), Query Frame = 0

Query: 1   MAPLLNDHFSYQTPENETVITKKIPRSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFLD 60
           MAPLLND FS+QTPE++TV TKKIP     NKYP WYSPDTGICRSVHASR +P+DPFLD
Sbjct: 1   MAPLLNDQFSFQTPESQTVQTKKIP-----NKYPGWYSPDTGICRSVHASRDLPTDPFLD 60

Query: 61  VVSFIFSFKHNGNSAIIDSSTGSSISYKEMFHMVKSMASGLQKFGISQGDVVLLVLPNSI 120
           V S+I SF+HNG SA+IDSSTG SISY+E++ MV SMASGLQK G+SQGDVVLL+LPNSI
Sbjct: 61  VASYILSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSI 120

Query: 121 FYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQAIGV 180
           FYPI+LLGVLYLGAV+TTMFPQSSS EIKKRI++CNVRLAF   Q + NFE+ G+QAIGV
Sbjct: 121 FYPIVLLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEAFGVQAIGV 180

Query: 181 PEITNFDLMQPMGFSYFHELISSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRN 240
           PE TN DLM+PMGFS F+ELIS G DL +RPVIRQEDTAA+LFSSGTTGVSKGV+LTHRN
Sbjct: 181 PENTNLDLMRPMGFSSFYELISGGFDLNKRPVIRQEDTAAILFSSGTTGVSKGVLLTHRN 240

Query: 241 FISTIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVK 300
           FIST+ELFVRFEASQYEYL TENVYLAAIPMFH+YGLSIFVMGLLSLGSS+V+MSKFDVK
Sbjct: 241 FISTVELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVK 300

Query: 301 EVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDFVKA 360
           EVV AIDRFKVTHFPVVPPIM  MARTA K G H+FRSLKQVSCGAASL+KK I DFV+A
Sbjct: 301 EVVVAIDRFKVTHFPVVPPIMIVMARTAVKFGGHKFRSLKQVSCGAASLSKKIIGDFVQA 360

Query: 361 FPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTG 420
            PHVDFIQGYGMTE+TA+GTRGFNTK A+NY SVGLLAPN EAKVVDWVSGS MPPGKTG
Sbjct: 361 LPHVDFIQGYGMTETTAVGTRGFNTKKAQNYLSVGLLAPNMEAKVVDWVSGSIMPPGKTG 420

Query: 421 ELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQ 480
           ELLLRGPG MKGYLNNPEATMSTID+ENWLHTGDIVYFDRDGYLYV+DRLKEVIKYKGFQ
Sbjct: 421 ELLLRGPGLMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQ 480

Query: 481 IAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAP 540
           IAPTDLE+VV+THPEVLDTAV AA+DEECGEIPVAFVVKKPGS LSQKDVIDYVAQQVAP
Sbjct: 481 IAPTDLESVVITHPEVLDTAVAAAEDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQQVAP 540

Query: 541 YKKVRKVVFTESIPKSAAGKVLRRELGKHLPSKL 575
           YKK+RKVVFTESIPKSAAGKVLR+ELGKHLPSKL
Sbjct: 541 YKKIRKVVFTESIPKSAAGKVLRKELGKHLPSKL 569

BLAST of Bhi05G000002 vs. NCBI nr
Match: XP_022963966.1 (4-coumarate--CoA ligase-like 6 isoform X1 [Cucurbita moschata])

HSP 1 Score: 978.4 bits (2528), Expect = 1.0e-281
Identity = 486/574 (84.67%), Postives = 528/574 (91.99%), Query Frame = 0

Query: 1   MAPLLNDHFSYQTPENETVITKKIPRSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFLD 60
           MAPLLND FS+QTPE++TV TKKIP     NKYP WYSPDTGICRSVHASR +P+DPFLD
Sbjct: 5   MAPLLNDQFSFQTPESQTVQTKKIP-----NKYPGWYSPDTGICRSVHASRDLPTDPFLD 64

Query: 61  VVSFIFSFKHNGNSAIIDSSTGSSISYKEMFHMVKSMASGLQKFGISQGDVVLLVLPNSI 120
           V S+I SF+HNG SA+IDSSTG SISY+E++ MV SMASGLQK G+SQGDVVLL+LPNSI
Sbjct: 65  VASYILSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSI 124

Query: 121 FYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQAIGV 180
           FYPI+LLGVLYLGAV+TTMFPQSSS EIKKRI++CNVRLAF   Q + NFE+ G+QAIGV
Sbjct: 125 FYPIVLLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEAFGVQAIGV 184

Query: 181 PEITNFDLMQPMGFSYFHELISSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTHRN 240
           PE TN DLM+PMGFS F+ELIS G DL +RPVIRQEDTAA+LFSSGTTGVSKGV+LTHRN
Sbjct: 185 PENTNLDLMRPMGFSSFYELISGGFDLNKRPVIRQEDTAAILFSSGTTGVSKGVLLTHRN 244

Query: 241 FISTIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFDVK 300
           FIST+ELFVRFEASQYEYL TENVYLAAIPMFH+YGLSIFVMGLLSLGSS+V+MSKFDVK
Sbjct: 245 FISTVELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVK 304

Query: 301 EVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDFVKA 360
           EVV AIDRFKVTHFPVVPPIM  MARTA K G H+FRSLKQVSCGAASL+KK I DFV+A
Sbjct: 305 EVVVAIDRFKVTHFPVVPPIMIVMARTAVKFGGHKFRSLKQVSCGAASLSKKIIGDFVQA 364

Query: 361 FPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGKTG 420
            PHVDFIQGYGMTE+TA+GTRGFNTK A+NY SVGLLAPN EAKVVDWVSGS MPPGKTG
Sbjct: 365 LPHVDFIQGYGMTETTAVGTRGFNTKKAQNYLSVGLLAPNMEAKVVDWVSGSIMPPGKTG 424

Query: 421 ELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKGFQ 480
           ELLLRGPG MKGYLNNPEATMSTID+ENWLHTGDIVYFDRDGYLYV+DRLKEVIKYKGFQ
Sbjct: 425 ELLLRGPGLMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQ 484

Query: 481 IAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQVAP 540
           IAPTDLE+VV+THPEVLDTAV AA+DEECGEIPVAFVVKKPGS LSQKDVIDYVAQQVAP
Sbjct: 485 IAPTDLESVVITHPEVLDTAVAAAEDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQQVAP 544

Query: 541 YKKVRKVVFTESIPKSAAGKVLRRELGKHLPSKL 575
           YKK+RKVVFTESIPKSAAGKVLR+ELGKHLPSKL
Sbjct: 545 YKKIRKVVFTESIPKSAAGKVLRKELGKHLPSKL 573