Cucsa.149800 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.149800 vs. Swiss-Prot
Match: 4CLL6_ARATH (4-coumarate--CoA ligase-like 6 OS=Arabidopsis thaliana GN=4CLL6 PE=2 SV=2)

HSP 1 Score: 640.6 bits (1651), Expect = 1.7e-182
Identity = 318/548 (58.03%), Postives = 422/548 (77.01%), Query Frame = 1

Query: 25  PRSERTNKYPN-WYSPDTGICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSST 84
           P+++ +++ P  W+S  TGI  S      LP DP LD VS +FS +  H+G +ALIDS T
Sbjct: 12  PKTQTSHQNPPFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFSHK--HHGDTALIDSLT 71

Query: 85  GNSISYKELFPMVKSMASGL-HNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMF 144
           G SIS+ EL  MV+SMA+G+ H LG+ QGDVV L+LPNS++FP+I L  + LGA+VTTM 
Sbjct: 72  GFSISHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMN 131

Query: 145 PQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMGFSSFYEL 204
           P SS  EIKK++++C+V LAF  T+ ++   +LG+  I V E+ +F+ +R      FY +
Sbjct: 132 PSSSLGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIRIEN-PKFYSI 191

Query: 205 ISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLP 264
           +      + KP+I+Q+D AAI++SSGTTG SKGV+L+HRN I+++ELFVRFEASQYEY  
Sbjct: 192 MKESFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPG 251

Query: 265 TKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPI 324
           + NVYLAA+P+ HIYGLS+FVMGL+SLGS++VVM +FD  DVV  I+RFK+THFPVVPP+
Sbjct: 252 SSNVYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPM 311

Query: 325 LTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVGT 384
           L AL + A+ +    F+SLKQVS GAA  S+K I++F+  LPHVD IQGYGMTESTAVGT
Sbjct: 312 LMALTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGT 371

Query: 385 RGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEAT 444
           RGFN++    Y SVGLLAPN +AKVVDW +GS +PPG  GEL ++GPG MKGYLNNP+AT
Sbjct: 372 RGFNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKAT 431

Query: 445 TFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDTA 504
             ++ +++WL TGDI YFD DGYL++VDR+KE+IKYKGFQIAP DLEAV+++HP ++D A
Sbjct: 432 QMSIVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAA 491

Query: 505 VAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGK 564
           V AA +EECGEIPVAFVV++  + L+++DV+ YVA QVAPY+KVRKV+   SIPKS  GK
Sbjct: 492 VTAAPNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGK 551

Query: 565 VLRRELQK 571
           +LR+EL++
Sbjct: 552 ILRKELKR 556

BLAST of Cucsa.149800 vs. Swiss-Prot
Match: 4CLL3_ORYSJ (4-coumarate--CoA ligase-like 3 OS=Oryza sativa subsp. japonica GN=4CLL3 PE=3 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.3e-145
Identity = 286/550 (52.00%), Postives = 366/550 (66.55%), Query Frame = 1

Query: 34  PNWYSPDTGICRSVHGYRKLPSDPFLDVVSFIFS-FQFQHNGHSALIDSSTGNSISYKEL 93
           P +YS  TGI  S+H    LP+DP L +V+ +F+       G   L+D++T +++S  +L
Sbjct: 25  PPFYSAATGIYSSIHPPVALPTDPSLTLVAHLFARLPLADPGAPTLVDAATASAVSRADL 84

Query: 94  FPMVKSMASGLHNL-GISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQSSSSEIK 153
             +V S+A+GL    G+ +G VVLL+LPNS+ FP+  L  L  GAV TTM P SS +EI 
Sbjct: 85  RRLVASLAAGLRRRHGVRKGSVVLLLLPNSVAFPVSFLAVLAAGAVATTMNPSSSPAEIA 144

Query: 154 KRITDCNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMG------FSSFYELISG 213
            +       L  A          L    + VPE  + +     G      F++F  ++ G
Sbjct: 145 AQARATGACLVLASRDGAARLPPLAAPVVLVPEILDHSAAADDGDDDQRVFAAFRAMLDG 204

Query: 214 GSD---LIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLP 273
           G         PV+ Q+D  AIL+SSGT+G SKGV+L+HRN I+  ELFVRFEASQY    
Sbjct: 205 GGGDGTETAVPVVGQDDAVAILYSSGTSGRSKGVVLTHRNLIAMTELFVRFEASQYHARG 264

Query: 274 TK-NVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPP 333
            + NVY+AA+PM H+YGLS+F +GL+S+G++VVVM +FD  D V AI R+KVTH P+VPP
Sbjct: 265 ARENVYMAALPMSHVYGLSLFAVGLLSIGATVVVMRRFDAGDAVAAIGRYKVTHMPLVPP 324

Query: 334 ILTALARTAEKIGV--HRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTA 393
           I+ A+ R A   GV   +  SL QVSCGAA  +   I  F+ A PHVDFIQGYGMTESTA
Sbjct: 325 IMAAMVRAAAAGGVPPSQVASLVQVSCGAAPITAALIHEFLQAFPHVDFIQGYGMTESTA 384

Query: 394 VGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNP 453
           VGTRGFNT   + Y SVGLLAPN  AK+V   + S +PPG +GEL L GPG MKGYL++ 
Sbjct: 385 VGTRGFNTSKHKKYTSVGLLAPNMHAKIVHLESSSCLPPGFSGELWLHGPGIMKGYLSDD 444

Query: 454 EATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVL 513
           +      D   WL TGDI YFD DGYLY+V RLK+ IKYKGFQIAP DLE V+I HPE+L
Sbjct: 445 DDACTRKD--GWLRTGDIAYFDLDGYLYIVGRLKDTIKYKGFQIAPGDLEEVLIHHPEIL 504

Query: 514 DTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSA 570
           D AV +A+DEE GEIPVAFVV++ GS L+ K V++YVA+QVAPYK+VRKV+F E+IPKS 
Sbjct: 505 DVAVTSAEDEEAGEIPVAFVVRRSGSNLSCKQVMEYVAKQVAPYKRVRKVVFVEAIPKSP 564

BLAST of Cucsa.149800 vs. Swiss-Prot
Match: 4CLL2_ORYSJ (4-coumarate--CoA ligase-like 2 OS=Oryza sativa subsp. japonica GN=4CLL2 PE=3 SV=3)

HSP 1 Score: 467.6 bits (1202), Expect = 1.9e-130
Identity = 262/515 (50.87%), Postives = 341/515 (66.21%), Query Frame = 1

Query: 35  NWYSPDTGICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFP 94
           ++YS  TG+  S+H    LPSDP L +V  +FS    H+ HS L+D+ T  ++S  +   
Sbjct: 14  SFYSAATGLYSSLHPPLPLPSDPSLSLVPHLFSHLPLHH-HSLLVDAPTAATLSCADFRR 73

Query: 95  MVKSMASGLHN-LGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKR 154
           +V S+A+GL   L I++G +VLL+LPNS+ FP+  L  L  GAV TTM P S+ +EI  R
Sbjct: 74  LVSSLAAGLRRRLHIARGSLVLLLLPNSLTFPVAFLAVLATGAVATTMNPSSAPAEIAAR 133

Query: 155 ITDCNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMGFSSFYELISGGSDL-IKK 214
           + D    L  A T        L    + VP+              F+ L+    +  ++ 
Sbjct: 134 LRDTAPSLVLASTHNAAKLPPLAAPLVLVPDTFQQQHDDDQFDFFFHALLETDPETPVEM 193

Query: 215 PV-IRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAAV 274
            V + Q+D AA+L+SSGT+G SKGV+++HRN I+ +ELFVRFEASQY      NVYLAA+
Sbjct: 194 GVGVGQDDAAAVLYSSGTSGRSKGVVVTHRNLIAMVELFVRFEASQYTRPARDNVYLAAL 253

Query: 275 PMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILTALARTAE 334
           PMFH+YGLS+F +GL+SLG +VVVM +F+V D VKAI ++KVTH P+VPPI++AL R   
Sbjct: 254 PMFHVYGLSLFAVGLLSLGCTVVVMRRFNVDDAVKAIRKYKVTHLPLVPPIMSALLRANP 313

Query: 335 KIGVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVGTRGFNTKNAR 394
            +      SL QVS GAA  +   I +F+HA PHVDFIQGYGMTESTAVGTRGFNT   +
Sbjct: 314 PL---ELDSLLQVSSGAAPLNHTLIHHFLHAFPHVDFIQGYGMTESTAVGTRGFNTCKHK 373

Query: 395 NYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMK------GYLNNPEATTFT 454
            Y SVGLLAPN  AK+V   +GS +PPG  GEL L GP  MK      GYLN+ +   FT
Sbjct: 374 KYASVGLLAPNMHAKIVHLESGSCLPPGSYGELWLHGPAIMKEFCFVTGYLNDDD-DAFT 433

Query: 455 MDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDTAVAA 514
             ++ WL TGDI YFD DGYL++V RLK+ IKYKGFQIAP DLEAV+I HPE++D AV +
Sbjct: 434 R-KDGWLRTGDIAYFDSDGYLFIVGRLKDTIKYKGFQIAPADLEAVLIRHPEIIDVAVTS 493

Query: 515 AKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV 541
            +DEE GEIPVAFVV+K GS L+   V++YVA+QV
Sbjct: 494 DEDEEAGEIPVAFVVRKSGSTLSCTHVMEYVAKQV 522

BLAST of Cucsa.149800 vs. Swiss-Prot
Match: 4CLL5_ARATH (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 439.5 bits (1129), Expect = 5.7e-122
Identity = 239/554 (43.14%), Postives = 351/554 (63.36%), Query Frame = 1

Query: 26  RSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGN 85
           RS   N    +YS  T I         LP +P LDV +FI S    H G  A ID+STG 
Sbjct: 7   RSGFCNSNSTFYSKRTPI--------PLPPNPSLDVTTFISSQA--HRGRIAFIDASTGQ 66

Query: 86  SISYKELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQS 145
           ++++ EL+  V+S+A  L  +GI +G VVLL+ PNSI FP++ L  + LGA++TT  P +
Sbjct: 67  NLTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 126

Query: 146 SSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMGFSSFYELISG 205
           +S+EI K+I D N  LAF  +Q +    A   K   V       LM      S  ++   
Sbjct: 127 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIV-------LMDEERVDSVGDVRRL 186

Query: 206 GSDLIKKPV-------IRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFV-RFEASQ 265
              + K+P        + Q+DTA +L+SSGTTG+SKGV+ SHRN I+ ++  V RF +  
Sbjct: 187 VEMMKKEPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDD 246

Query: 266 YEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFP 325
            E       ++  VPMFHIYGL+ F  GL++ GS+++V+SKF++ +++ AI +++ T  P
Sbjct: 247 GEQR-----FICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLP 306

Query: 326 VVPPILTALARTAEKIGV-HRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTE 385
           +VPPIL A+   A++I   +   S+  V CG A  SK+  + F    P V  +QGYG+TE
Sbjct: 307 LVPPILVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTE 366

Query: 386 STAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYL 445
           ST +G      + +R Y + G L+ + E ++VD VTG  + P +TGEL L+GP  MKGY 
Sbjct: 367 STGIGASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYF 426

Query: 446 NNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHP 505
           +N EAT+ T+D E WL TGD+ Y D DG+++VVDRLKE+IKYKG+Q+AP +LEA+++THP
Sbjct: 427 SNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHP 486

Query: 506 EVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIP 565
           E+ D AV    D+E G+ P+A+VV+K GS+L++K ++++VA+QVAPYK++RKV F  SIP
Sbjct: 487 EITDAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIP 538

Query: 566 KSAAGKVLRRELQK 571
           K+ +GK+LR++L K
Sbjct: 547 KNPSGKILRKDLIK 538

BLAST of Cucsa.149800 vs. Swiss-Prot
Match: 4CLL7_ARATH (4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana GN=4CLL7 PE=1 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 4.2e-117
Identity = 227/535 (42.43%), Postives = 338/535 (63.18%), Query Frame = 1

Query: 42  GICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMAS 101
           GI RS+     LP DP   +VSF+F     +    A+ DS TG+S+++ +L   V  +A 
Sbjct: 10  GIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAH 69

Query: 102 GLHNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRL 161
           G H LGI + DVVL+  PNS  FP+  L    +G V TT  P  + +E+ K+I D N ++
Sbjct: 70  GFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKI 129

Query: 162 AFAITQ---KIKNFEA----LGIK-TIGVPENTNFNLMRSMGFSSFYELISGGSDLIKKP 221
             ++ Q   KIK F+     LG K T+ +P  +N  ++    F +  EL    S+     
Sbjct: 130 IISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKIL---SFDNVMELSEPVSEY-PFV 189

Query: 222 VIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAAVPM 281
            I+Q DTAA+L+SSGTTG SKGV L+H NFI+   +    +    EY     V+L  +PM
Sbjct: 190 EIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEY---HGVFLCFLPM 249

Query: 282 FHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILTALARTAEKI 341
           FH++GL++     +  G+++V M++F+++ V+K I++F+VTH  VVPP+  AL++ +  +
Sbjct: 250 FHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSI-V 309

Query: 342 GVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVGTRGFNTKNARNY 401
                 SLK +  GAA   K  ++     +P+V  +QGYGMTE+  + +        RN 
Sbjct: 310 KKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGKRNS 369

Query: 402 LSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATTFTMDQENWLH 461
            S G+LAP  EA++V   TG S PP + GE+ +RGP  MKGYLNNP+AT  T+D+++W+H
Sbjct: 370 GSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVH 429

Query: 462 TGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGE 521
           TGD+ YF+ DG LYVVDR+KE+IKYKGFQ+AP +LE ++++HP++LD  V    DEE GE
Sbjct: 430 TGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGE 489

Query: 522 IPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGKVLRREL 569
           +P+AFVV+ P S++T++D+  ++A+QVAPYK++R+V F   +PKSAAGK+LRREL
Sbjct: 490 VPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRREL 536

BLAST of Cucsa.149800 vs. TrEMBL
Match: A0A0A0L8X1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G638510 PE=4 SV=1)

HSP 1 Score: 1141.3 bits (2951), Expect = 0.0e+00
Identity = 577/577 (100.00%), Postives = 577/577 (100.00%), Query Frame = 1

Query: 1   MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD 60
           MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD
Sbjct: 1   MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD 60

Query: 61  VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN 120
           VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN
Sbjct: 61  VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN 120

Query: 121 SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI 180
           SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI
Sbjct: 121 SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI 180

Query: 181 GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH 240
           GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH
Sbjct: 181 GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH 240

Query: 241 RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD 300
           RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD
Sbjct: 241 RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD 300

Query: 301 VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV 360
           VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV
Sbjct: 301 VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV 360

Query: 361 HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK 420
           HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK
Sbjct: 361 HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK 420

Query: 421 TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG 480
           TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG
Sbjct: 421 TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG 480

Query: 481 FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV 540
           FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV
Sbjct: 481 FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV 540

Query: 541 APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL 578
           APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL
Sbjct: 541 APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL 577

BLAST of Cucsa.149800 vs. TrEMBL
Match: S4W608_CHOAR (Ca4CL9 OS=Chosenia arbutifolia PE=2 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 6.4e-205
Identity = 364/556 (65.47%), Postives = 440/556 (79.14%), Query Frame = 1

Query: 22  KKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDS 81
           +K+  +     +P+WYSP+TGI  S H    LP+DPFLDVVSFIFS    HNG +ALIDS
Sbjct: 2   EKLTATASKTTFPDWYSPETGIYSSKHPTIPLPADPFLDVVSFIFSHH-NHNGLTALIDS 61

Query: 82  STGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTM 141
           S+G SISY ++ P+VKS+ASGL N+GI QGDVVLL+LPNSI FPI+ LG LYLG +V+TM
Sbjct: 62  SSGFSISYSKILPLVKSVASGLRNMGIKQGDVVLLLLPNSIHFPIVFLGVLYLGGIVSTM 121

Query: 142 FPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMGFSSFYE 201
            P SS  E+KKRI DCN R+AF   +K+  F+ LGI  IGV EN N +  +S  F +FY 
Sbjct: 122 NPLSSEPEVKKRIADCNARIAFVELKKVGKFQPLGILVIGVQENVNLD-EKSNDFEAFYM 181

Query: 202 LISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYL 261
            +    D+  KPVIRQ+DTAAI++SSGTT  SKGV+L+HRNFIS +ELFV+FEASQYEY 
Sbjct: 182 RLYSKGDVGVKPVIRQQDTAAIMYSSGTTAASKGVVLTHRNFISMVELFVKFEASQYEYS 241

Query: 262 PTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPP 321
            T NVYLA +PMFHIYGLS+FV+GL+SLGSS+VVM KFDV + VK IDR+ VTHFPVVPP
Sbjct: 242 STDNVYLAVLPMFHIYGLSLFVVGLLSLGSSIVVMRKFDVSETVKVIDRYGVTHFPVVPP 301

Query: 322 ILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVG 381
           ILTAL RTA+ +  +  + LK VSCGAA    KTI +FV  LPHVDFIQGYG+TESTAVG
Sbjct: 302 ILTALTRTAKGVCGNSLKCLKVVSCGAAPLFAKTIQDFVEVLPHVDFIQGYGLTESTAVG 361

Query: 382 TRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEA 441
           TRG NT+  + Y S+GLLAPN EAKVVDW+TG+ +PPG +GEL +RGPG MK YLN+ EA
Sbjct: 362 TRGLNTEKFKKYSSIGLLAPNIEAKVVDWITGTLLPPGGSGELWIRGPGVMKEYLNDGEA 421

Query: 442 TTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDT 501
           T  T+D++ WLHTGD+VY D DGYLYVVDRLKE+IKYKGFQIAP DLEAV+I+H E+LD 
Sbjct: 422 TALTIDKDGWLHTGDVVYTDHDGYLYVVDRLKEIIKYKGFQIAPADLEAVLISHCEILDA 481

Query: 502 AVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAG 561
           AV  A D+ECGEIPVAFVVK+ GS +T++ +++YVA QVAPYKKVRKVIFT+SIPKSAAG
Sbjct: 482 AVIPAIDKECGEIPVAFVVKRKGSMVTKEAIINYVADQVAPYKKVRKVIFTQSIPKSAAG 541

Query: 562 KVLRRELQKHFPASKL 578
           K+LRREL K+   SKL
Sbjct: 542 KILRREL-KNSLTSKL 554

BLAST of Cucsa.149800 vs. TrEMBL
Match: B9GZT7_POPTR (4-coumarate--CoA ligase family protein OS=Populus trichocarpa GN=POPTR_0003s09880g PE=4 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 3.9e-202
Identity = 359/548 (65.51%), Postives = 430/548 (78.47%), Query Frame = 1

Query: 22  KKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDS 81
           +K+  +      P WYSP+TGI  S H    LP+DPFLDVVSFIFS    HNG +ALIDS
Sbjct: 2   EKLTATASKTTLPEWYSPETGIYSSKHPPIPLPADPFLDVVSFIFSHH-NHNGLTALIDS 61

Query: 82  STGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTM 141
           S+G SISY ++ P+VKS+ASGL N+GI QGDVVLL+LPNSI FPI+ LG LYLG +V+TM
Sbjct: 62  SSGFSISYSKILPLVKSVASGLSNMGIKQGDVVLLLLPNSIHFPIVFLGVLYLGGIVSTM 121

Query: 142 FPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMGFSSFYE 201
            P SS  E+K+RI DCN  +AF   +K+  F+ LGI  IGVPEN NF+           E
Sbjct: 122 NPLSSELEVKQRIVDCNACIAFVELEKVCKFQPLGIPVIGVPENVNFD-----------E 181

Query: 202 LISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYL 261
            I    D+  KPVIRQ+DTAAI++SSGTT  SKGV+L+HRNFIS +ELFV+FEASQYEY 
Sbjct: 182 KIYSKGDVGVKPVIRQQDTAAIMYSSGTTAASKGVVLTHRNFISMVELFVKFEASQYEYS 241

Query: 262 PTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPP 321
            T NVYLA +PMFHIYGLS+FV+GL+SLGSS+VVM KFDV ++VK IDR+ VTHFPVVPP
Sbjct: 242 STDNVYLAVLPMFHIYGLSLFVVGLLSLGSSIVVMRKFDVSEMVKVIDRYGVTHFPVVPP 301

Query: 322 ILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVG 381
           ILTAL RTA+ +  +  + LK VSCGAA    KTI +FV  LPHVDFIQGYG+TESTAVG
Sbjct: 302 ILTALTRTAKGVCGNSLKCLKLVSCGAAPLFGKTIQDFVEVLPHVDFIQGYGLTESTAVG 361

Query: 382 TRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEA 441
           TRG NT+  + Y S+GLLAPN EAKVVDW+TG+ +PPG +GEL +RGPG MK YLN  EA
Sbjct: 362 TRGLNTEKFQKYSSIGLLAPNIEAKVVDWITGALLPPGGSGELWIRGPGVMKEYLNGGEA 421

Query: 442 TTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDT 501
           T  T+D++ WLHTGD+VY D DGYLYVVDRLKE+IKYKGFQIAP DLEAV+I+H E+LD 
Sbjct: 422 TALTIDKDGWLHTGDVVYADHDGYLYVVDRLKEIIKYKGFQIAPADLEAVLISHCEILDA 481

Query: 502 AVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAG 561
           AV    D+ECGEIPVAFVVK+ GS LTQ+ +++YVA+QVAPYKKVRKVIFT+SIPKSAAG
Sbjct: 482 AVIPVVDKECGEIPVAFVVKRQGSMLTQEAIINYVAEQVAPYKKVRKVIFTQSIPKSAAG 537

Query: 562 KVLRRELQ 570
           K+LRREL+
Sbjct: 542 KILRRELK 537

BLAST of Cucsa.149800 vs. TrEMBL
Match: A0A061G6U1_THECC (Acyl:coa ligase OS=Theobroma cacao GN=TCM_016640 PE=4 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 6.6e-202
Identity = 361/577 (62.56%), Postives = 451/577 (78.16%), Query Frame = 1

Query: 1   MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD 60
           MA  +N+ FSF+  E+            +T ++P+WYSP+TGI  S H    LP+D FLD
Sbjct: 1   MATSLNSHFSFQRSES------------KTTRFPDWYSPETGIYSSKHSSISLPTDHFLD 60

Query: 61  VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN 120
           +VSFIFS Q  H+G +ALIDSS+G S SY +L P+++SMASGLH+LG+SQGDVVLL+LPN
Sbjct: 61  IVSFIFSHQ--HDGVTALIDSSSGYSTSYAKLLPLIQSMASGLHHLGVSQGDVVLLLLPN 120

Query: 121 SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI 180
           S+ FPII L  LYLGA+VTTM P +S  EIKK+I D +VR AF +  K+   + LG+  I
Sbjct: 121 SVHFPIIFLSVLYLGAIVTTMNPLTSMLEIKKQIADSSVRFAFTLLDKVDKLKNLGVHAI 180

Query: 181 GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH 240
           GVPEN N +  ++  F  F++LI G  D   +PVIRQ+DTAAI++SSGTTGVSKGV+L+H
Sbjct: 181 GVPENLNLDSEKT-DFLLFHKLIGGQFDKAPRPVIRQQDTAAIMYSSGTTGVSKGVVLTH 240

Query: 241 RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD 300
            NFI+ +ELFVRFEASQYEY  +KNVYLA +PMFHIYGLS+FV+GL+SLG+S+VVM +F+
Sbjct: 241 GNFIAMVELFVRFEASQYEYSSSKNVYLAVLPMFHIYGLSLFVVGLLSLGTSIVVMRRFN 300

Query: 301 VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV 360
             ++V+ ID++ VTHFPVVPPIL  LA  A+ +  +  +SLKQVSCGAA  S+K I++FV
Sbjct: 301 ATEMVRVIDKYGVTHFPVVPPILRTLAMRAKGVCENSLKSLKQVSCGAAPLSRKAIEDFV 360

Query: 361 HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK 420
            ALPHVDFIQGYGMTESTAVG RGFN +    Y SVGLLAPN +AKVVDW +GSS+PP  
Sbjct: 361 QALPHVDFIQGYGMTESTAVGNRGFNNEKHHKYSSVGLLAPNMQAKVVDWNSGSSLPPDS 420

Query: 421 TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG 480
            GEL LRGPG M+GYLNN EAT  T+D++ WL TGDIV FD DGY+YV DRLKE+IKYKG
Sbjct: 421 HGELWLRGPGLMQGYLNNVEATRMTIDKDGWLRTGDIVCFDEDGYMYVSDRLKEIIKYKG 480

Query: 481 FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV 540
           +QIAP DLEA++I+HPE+LD AV  A DEE GEIPVAFVV++ GS LTQ  V+D+VA+QV
Sbjct: 481 YQIAPADLEAILISHPEILDAAVIPAIDEEAGEIPVAFVVRRQGSTLTQGAVMDFVAKQV 540

Query: 541 APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL 578
           APYKKVRKV+FT+SIPKSAAGK LRREL ++F +S+L
Sbjct: 541 APYKKVRKVVFTKSIPKSAAGKTLRREL-RNFLSSRL 561

BLAST of Cucsa.149800 vs. TrEMBL
Match: A0A0D2S1Q6_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_004G180900 PE=4 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 2.1e-200
Identity = 356/549 (64.85%), Postives = 437/549 (79.60%), Query Frame = 1

Query: 29  RTNKYPNWYSPDTGICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSIS 88
           +T   P+WYSP+T I  S +    LP+DPFLD+VSFIFS Q  H+G +ALIDSS+G SIS
Sbjct: 15  KTTHLPDWYSPETRIYSSTYSSISLPTDPFLDIVSFIFSHQ--HDGVTALIDSSSGYSIS 74

Query: 89  YKELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQSSSS 148
           Y +L P+V+SMASGLH+LG+SQGDVVLL+LPNS+ FPII    LYLGAVVTTM P S+  
Sbjct: 75  YSKLLPLVQSMASGLHHLGVSQGDVVLLLLPNSVNFPIIFFSVLYLGAVVTTMNPLSNMM 134

Query: 149 EIKKRITDCNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMGFSSFYELISGGSD 208
           E+KK+I DC VR AF + + +   + LG+  IGVPEN + +  ++ GF SF +LI G   
Sbjct: 135 EVKKQIVDCGVRFAFTLLENVDKLQKLGVHAIGVPENMDLDSEKT-GFLSFRKLIGGQFG 194

Query: 209 LIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYL 268
              +PVIRQ+DTAAI++SSGTTGVSKGV+L+H NFI+T ELFVRFEASQYEY  +KNVYL
Sbjct: 195 KAPRPVIRQQDTAAIMYSSGTTGVSKGVVLTHGNFIATTELFVRFEASQYEYSSSKNVYL 254

Query: 269 AAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILTALAR 328
           A +PMFHIYGLS+FV+GL+SLGSS+V+M +FD  ++VK ID + VTHFPVVPPILT L  
Sbjct: 255 AVLPMFHIYGLSLFVVGLLSLGSSIVIMRRFDASELVKVIDDYGVTHFPVVPPILTTLTM 314

Query: 329 TAEKIGVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVGTRGFNTK 388
            A+ +  +  +SLKQVSCGAA  S KTI++FV ALPHVDFIQGYGMTESTAVGTRGFN +
Sbjct: 315 RAKHVCENSLQSLKQVSCGAAPLSGKTIEDFVQALPHVDFIQGYGMTESTAVGTRGFNNE 374

Query: 389 NARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATTFTMDQ 448
              NY S+GLLAPN +AKVVDW +GSS+PPG  GEL LRGP  M+ Y+NN EAT  T+D+
Sbjct: 375 KHHNYSSIGLLAPNMQAKVVDWNSGSSLPPGFHGELWLRGPAIMQRYINNVEATNMTIDK 434

Query: 449 ENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDTAVAAAKD 508
           + WL TGDIV FD +GYLY+ DRLKE+IKYKG+QIAP DLEAV+I+HPE+LD AV +A D
Sbjct: 435 DGWLRTGDIVCFDGEGYLYLSDRLKEIIKYKGYQIAPADLEAVLISHPEILDAAVTSAAD 494

Query: 509 EECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGKVLRREL 568
           E CGEIPVAFVV++ GS LT+  V+D+VA QVAPYKKVR+V+FTESIPKSAAGK LR+EL
Sbjct: 495 EVCGEIPVAFVVRRHGSTLTKGAVIDFVANQVAPYKKVRRVVFTESIPKSAAGKNLRKEL 554

Query: 569 QKHFPASKL 578
            K F +S+L
Sbjct: 555 -KSFISSRL 559

BLAST of Cucsa.149800 vs. TAIR10
Match: AT4G19010.1 (AT4G19010.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 640.6 bits (1651), Expect = 9.5e-184
Identity = 318/548 (58.03%), Postives = 422/548 (77.01%), Query Frame = 1

Query: 25  PRSERTNKYPN-WYSPDTGICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSST 84
           P+++ +++ P  W+S  TGI  S      LP DP LD VS +FS +  H+G +ALIDS T
Sbjct: 12  PKTQTSHQNPPFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFSHK--HHGDTALIDSLT 71

Query: 85  GNSISYKELFPMVKSMASGL-HNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMF 144
           G SIS+ EL  MV+SMA+G+ H LG+ QGDVV L+LPNS++FP+I L  + LGA+VTTM 
Sbjct: 72  GFSISHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMN 131

Query: 145 PQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMGFSSFYEL 204
           P SS  EIKK++++C+V LAF  T+ ++   +LG+  I V E+ +F+ +R      FY +
Sbjct: 132 PSSSLGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIRIEN-PKFYSI 191

Query: 205 ISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLP 264
           +      + KP+I+Q+D AAI++SSGTTG SKGV+L+HRN I+++ELFVRFEASQYEY  
Sbjct: 192 MKESFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPG 251

Query: 265 TKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPI 324
           + NVYLAA+P+ HIYGLS+FVMGL+SLGS++VVM +FD  DVV  I+RFK+THFPVVPP+
Sbjct: 252 SSNVYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPM 311

Query: 325 LTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVGT 384
           L AL + A+ +    F+SLKQVS GAA  S+K I++F+  LPHVD IQGYGMTESTAVGT
Sbjct: 312 LMALTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGT 371

Query: 385 RGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEAT 444
           RGFN++    Y SVGLLAPN +AKVVDW +GS +PPG  GEL ++GPG MKGYLNNP+AT
Sbjct: 372 RGFNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKAT 431

Query: 445 TFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDTA 504
             ++ +++WL TGDI YFD DGYL++VDR+KE+IKYKGFQIAP DLEAV+++HP ++D A
Sbjct: 432 QMSIVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAA 491

Query: 505 VAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGK 564
           V AA +EECGEIPVAFVV++  + L+++DV+ YVA QVAPY+KVRKV+   SIPKS  GK
Sbjct: 492 VTAAPNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGK 551

Query: 565 VLRRELQK 571
           +LR+EL++
Sbjct: 552 ILRKELKR 556

BLAST of Cucsa.149800 vs. TAIR10
Match: AT1G20510.1 (AT1G20510.1 OPC-8:0 CoA ligase1)

HSP 1 Score: 439.5 bits (1129), Expect = 3.2e-123
Identity = 239/554 (43.14%), Postives = 351/554 (63.36%), Query Frame = 1

Query: 26  RSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGN 85
           RS   N    +YS  T I         LP +P LDV +FI S    H G  A ID+STG 
Sbjct: 7   RSGFCNSNSTFYSKRTPI--------PLPPNPSLDVTTFISSQA--HRGRIAFIDASTGQ 66

Query: 86  SISYKELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQS 145
           ++++ EL+  V+S+A  L  +GI +G VVLL+ PNSI FP++ L  + LGA++TT  P +
Sbjct: 67  NLTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 126

Query: 146 SSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMGFSSFYELISG 205
           +S+EI K+I D N  LAF  +Q +    A   K   V       LM      S  ++   
Sbjct: 127 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIV-------LMDEERVDSVGDVRRL 186

Query: 206 GSDLIKKPV-------IRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFV-RFEASQ 265
              + K+P        + Q+DTA +L+SSGTTG+SKGV+ SHRN I+ ++  V RF +  
Sbjct: 187 VEMMKKEPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDD 246

Query: 266 YEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFP 325
            E       ++  VPMFHIYGL+ F  GL++ GS+++V+SKF++ +++ AI +++ T  P
Sbjct: 247 GEQR-----FICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLP 306

Query: 326 VVPPILTALARTAEKIGV-HRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTE 385
           +VPPIL A+   A++I   +   S+  V CG A  SK+  + F    P V  +QGYG+TE
Sbjct: 307 LVPPILVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTE 366

Query: 386 STAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYL 445
           ST +G      + +R Y + G L+ + E ++VD VTG  + P +TGEL L+GP  MKGY 
Sbjct: 367 STGIGASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYF 426

Query: 446 NNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHP 505
           +N EAT+ T+D E WL TGD+ Y D DG+++VVDRLKE+IKYKG+Q+AP +LEA+++THP
Sbjct: 427 SNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHP 486

Query: 506 EVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIP 565
           E+ D AV    D+E G+ P+A+VV+K GS+L++K ++++VA+QVAPYK++RKV F  SIP
Sbjct: 487 EITDAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIP 538

Query: 566 KSAAGKVLRRELQK 571
           K+ +GK+LR++L K
Sbjct: 547 KNPSGKILRKDLIK 538

BLAST of Cucsa.149800 vs. TAIR10
Match: AT4G05160.1 (AT4G05160.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 423.3 bits (1087), Expect = 2.4e-118
Identity = 227/535 (42.43%), Postives = 338/535 (63.18%), Query Frame = 1

Query: 42  GICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMAS 101
           GI RS+     LP DP   +VSF+F     +    A+ DS TG+S+++ +L   V  +A 
Sbjct: 10  GIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAH 69

Query: 102 GLHNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRL 161
           G H LGI + DVVL+  PNS  FP+  L    +G V TT  P  + +E+ K+I D N ++
Sbjct: 70  GFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKI 129

Query: 162 AFAITQ---KIKNFEA----LGIK-TIGVPENTNFNLMRSMGFSSFYELISGGSDLIKKP 221
             ++ Q   KIK F+     LG K T+ +P  +N  ++    F +  EL    S+     
Sbjct: 130 IISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKIL---SFDNVMELSEPVSEY-PFV 189

Query: 222 VIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAAVPM 281
            I+Q DTAA+L+SSGTTG SKGV L+H NFI+   +    +    EY     V+L  +PM
Sbjct: 190 EIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEY---HGVFLCFLPM 249

Query: 282 FHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILTALARTAEKI 341
           FH++GL++     +  G+++V M++F+++ V+K I++F+VTH  VVPP+  AL++ +  +
Sbjct: 250 FHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSI-V 309

Query: 342 GVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVGTRGFNTKNARNY 401
                 SLK +  GAA   K  ++     +P+V  +QGYGMTE+  + +        RN 
Sbjct: 310 KKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGKRNS 369

Query: 402 LSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATTFTMDQENWLH 461
            S G+LAP  EA++V   TG S PP + GE+ +RGP  MKGYLNNP+AT  T+D+++W+H
Sbjct: 370 GSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVH 429

Query: 462 TGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGE 521
           TGD+ YF+ DG LYVVDR+KE+IKYKGFQ+AP +LE ++++HP++LD  V    DEE GE
Sbjct: 430 TGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGE 489

Query: 522 IPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGKVLRREL 569
           +P+AFVV+ P S++T++D+  ++A+QVAPYK++R+V F   +PKSAAGK+LRREL
Sbjct: 490 VPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRREL 536

BLAST of Cucsa.149800 vs. TAIR10
Match: AT1G20480.1 (AT1G20480.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 406.4 bits (1043), Expect = 3.0e-113
Identity = 222/544 (40.81%), Postives = 331/544 (60.85%), Query Frame = 1

Query: 37  YSPDTGICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMV 96
           +   T I  S      LP + FLDV SFI S    H G +  +D+ TG  +S+ EL+  V
Sbjct: 27  FCESTSIFYSKREPMALPPNQFLDVTSFIASQP--HRGKTVFVDAVTGRRLSFPELWLGV 86

Query: 97  KSMASGLHNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITD 156
           + +A  L+ LG+ +G+VV+++ PNSI FPI+ L  + LGA++TT  P ++S EI K+I D
Sbjct: 87  ERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEISKQIGD 146

Query: 157 CNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMGFSSFYELISGGSDLIK----- 216
               LAF   + +    A     + V    ++++  S  +    +L+     +I+     
Sbjct: 147 SRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVP-SQSYGDRVKLVGRLETMIETEPSE 206

Query: 217 ---KPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELF-VRFEASQYEYLPTKNVY 276
              K  + Q+DTAA+L+SSGTTG SKGVMLSHRN I+ ++ +  RF   Q          
Sbjct: 207 SRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRARFGLEQRT-------- 266

Query: 277 LAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILTALA 336
           +  +PM HI+G   F  GL++LG ++VV+ KFD+  ++ A++  + ++  +VPPI+ A+ 
Sbjct: 267 ICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMV 326

Query: 337 RTAEKIGV-HRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVGTRGFN 396
             A +I   +   SL  V  G A  S++  + FV   P V  +QGYG+TESTA+    FN
Sbjct: 327 NGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFN 386

Query: 397 TKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATTFTM 456
            +  + Y + GLLAPN E K+VD  TG  +   +TGEL +R P  MKGY  N EAT  T+
Sbjct: 387 KEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTI 446

Query: 457 DQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDTAVAAA 516
           D E WL TGD+ Y D DG+++VVDRLKE+IK  G+Q+AP +LEA+++ HPE+ D AV   
Sbjct: 447 DSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPI 506

Query: 517 KDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGKVLRR 571
            D + G+ P+A++V+K GS L++ +++ +VA+QV+PYKK+RKV F  SIPK+ +GK+LRR
Sbjct: 507 PDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILRR 559

BLAST of Cucsa.149800 vs. TAIR10
Match: AT1G20500.1 (AT1G20500.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 402.9 bits (1034), Expect = 3.3e-112
Identity = 223/548 (40.69%), Postives = 334/548 (60.95%), Query Frame = 1

Query: 39  PDTGICRSVHGYRK------LPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSISYKEL 98
           P +G C+S   +        LP +   DV +FI S    H G +A ID++TG  +++ +L
Sbjct: 12  PRSGFCKSNSTFYSKRQPLSLPPNLSRDVTTFISSQP--HRGKTAFIDAATGQCLTFSDL 71

Query: 99  FPMVKSMASGL-HNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQSSSSEIK 158
           +  V  +A  L H +GI +GDVVL++ PNSIF P++ L  + LGAV TT    ++S EI 
Sbjct: 72  WRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEIS 131

Query: 159 KRITDCNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMGFSSFYELISGGSDLIK 218
           K+I D N  L F  T+++     + I  +   +     L      +S   ++   S+++K
Sbjct: 132 KQIADSNPTLVFT-TRQLAPKLPVAISVVLTDDEVYQEL------TSAIRVVGILSEMVK 191

Query: 219 KPV--------IRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPT 278
           K          + Q+DTA +L+SSGTTG SKGV+ SHRN  + +  F+       + L  
Sbjct: 192 KEPSGQRVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFIS------DNLKR 251

Query: 279 KNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPIL 338
            ++++  VPMFH YGL  F MG ++LGS+VV++ +F + D++ A+++ + T   + PP+L
Sbjct: 252 DDIFICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVL 311

Query: 339 TALARTAEKIGV-HRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVGT 398
            A+   A+ I   +   SLK V CG A  SK+  + F+   P VD +QGY +TES   G 
Sbjct: 312 VAMINDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGGA 371

Query: 399 RGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEAT 458
              + + +R Y + G L  + EA++VD  TG  M   +TGEL L+GP   KGY  N EAT
Sbjct: 372 FTNSAEESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEAT 431

Query: 459 TFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDTA 518
             T++ E WL TGD+ Y D DG+L+VVDRLKE+IKYKG+Q+ P +LEA++ITHP++LD A
Sbjct: 432 NETINLEGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDAA 491

Query: 519 VAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGK 571
           V    D+E G+ P+A+VV+K  S L++K V+D++++QVAPYKK+RKV F  SIPK+A+GK
Sbjct: 492 VIPFPDKEAGQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTASGK 544

BLAST of Cucsa.149800 vs. NCBI nr
Match: gi|449453328|ref|XP_004144410.1| (PREDICTED: 4-coumarate--CoA ligase-like 6 [Cucumis sativus])

HSP 1 Score: 1141.3 bits (2951), Expect = 0.0e+00
Identity = 577/577 (100.00%), Postives = 577/577 (100.00%), Query Frame = 1

Query: 1   MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD 60
           MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD
Sbjct: 1   MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD 60

Query: 61  VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN 120
           VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN
Sbjct: 61  VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN 120

Query: 121 SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI 180
           SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI
Sbjct: 121 SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI 180

Query: 181 GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH 240
           GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH
Sbjct: 181 GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH 240

Query: 241 RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD 300
           RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD
Sbjct: 241 RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD 300

Query: 301 VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV 360
           VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV
Sbjct: 301 VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV 360

Query: 361 HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK 420
           HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK
Sbjct: 361 HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK 420

Query: 421 TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG 480
           TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG
Sbjct: 421 TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG 480

Query: 481 FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV 540
           FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV
Sbjct: 481 FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV 540

Query: 541 APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL 578
           APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL
Sbjct: 541 APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL 577

BLAST of Cucsa.149800 vs. NCBI nr
Match: gi|659120993|ref|XP_008460452.1| (PREDICTED: 4-coumarate--CoA ligase-like 6 [Cucumis melo])

HSP 1 Score: 1011.5 bits (2614), Expect = 5.8e-292
Identity = 512/552 (92.75%), Postives = 527/552 (95.47%), Query Frame = 1

Query: 1   MARLI-NNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFL 60
           MARL+ N+QFSFETPENEA ITKKIP SERTNKYPNWYSPDTGIC SVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS QFQHNGHSALIDS TGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKT 180
           NSIF+PIIILGALYLGAV+TTMFPQSSSSEIKKRITDCNVRLAF ITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTNF+LMR MGFSSFYELIS GSDLIK+PVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNF 360
           DVKDVVKAIDRFKVTHFPVVPPIL AL+RTAEKIGV RFRSLKQVSCGAAASSKKTID+F
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VHALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           V AL HVDFIQGYGMTESTA+GTRGFN K  RNYLSVGLLAPNTEAKVVDWVTGSSMPPG
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEAT  T+DQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQ 540
           GFQIAPTDLEAVVI HPEVLDTAVAAAKDEECGEIPVAFVVKKPGSAL+QKDVVDYVAQQ
Sbjct: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540

Query: 541 VAPYKKVRKVIF 552
           V+   ++   IF
Sbjct: 541 VSHIFQICPCIF 552

BLAST of Cucsa.149800 vs. NCBI nr
Match: gi|516561183|gb|AGO89326.1| (Ca4CL9 [Salix arbutifolia])

HSP 1 Score: 721.8 bits (1862), Expect = 9.2e-205
Identity = 364/556 (65.47%), Postives = 440/556 (79.14%), Query Frame = 1

Query: 22  KKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDS 81
           +K+  +     +P+WYSP+TGI  S H    LP+DPFLDVVSFIFS    HNG +ALIDS
Sbjct: 2   EKLTATASKTTFPDWYSPETGIYSSKHPTIPLPADPFLDVVSFIFSHH-NHNGLTALIDS 61

Query: 82  STGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTM 141
           S+G SISY ++ P+VKS+ASGL N+GI QGDVVLL+LPNSI FPI+ LG LYLG +V+TM
Sbjct: 62  SSGFSISYSKILPLVKSVASGLRNMGIKQGDVVLLLLPNSIHFPIVFLGVLYLGGIVSTM 121

Query: 142 FPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMGFSSFYE 201
            P SS  E+KKRI DCN R+AF   +K+  F+ LGI  IGV EN N +  +S  F +FY 
Sbjct: 122 NPLSSEPEVKKRIADCNARIAFVELKKVGKFQPLGILVIGVQENVNLD-EKSNDFEAFYM 181

Query: 202 LISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYL 261
            +    D+  KPVIRQ+DTAAI++SSGTT  SKGV+L+HRNFIS +ELFV+FEASQYEY 
Sbjct: 182 RLYSKGDVGVKPVIRQQDTAAIMYSSGTTAASKGVVLTHRNFISMVELFVKFEASQYEYS 241

Query: 262 PTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPP 321
            T NVYLA +PMFHIYGLS+FV+GL+SLGSS+VVM KFDV + VK IDR+ VTHFPVVPP
Sbjct: 242 STDNVYLAVLPMFHIYGLSLFVVGLLSLGSSIVVMRKFDVSETVKVIDRYGVTHFPVVPP 301

Query: 322 ILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVG 381
           ILTAL RTA+ +  +  + LK VSCGAA    KTI +FV  LPHVDFIQGYG+TESTAVG
Sbjct: 302 ILTALTRTAKGVCGNSLKCLKVVSCGAAPLFAKTIQDFVEVLPHVDFIQGYGLTESTAVG 361

Query: 382 TRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEA 441
           TRG NT+  + Y S+GLLAPN EAKVVDW+TG+ +PPG +GEL +RGPG MK YLN+ EA
Sbjct: 362 TRGLNTEKFKKYSSIGLLAPNIEAKVVDWITGTLLPPGGSGELWIRGPGVMKEYLNDGEA 421

Query: 442 TTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDT 501
           T  T+D++ WLHTGD+VY D DGYLYVVDRLKE+IKYKGFQIAP DLEAV+I+H E+LD 
Sbjct: 422 TALTIDKDGWLHTGDVVYTDHDGYLYVVDRLKEIIKYKGFQIAPADLEAVLISHCEILDA 481

Query: 502 AVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAG 561
           AV  A D+ECGEIPVAFVVK+ GS +T++ +++YVA QVAPYKKVRKVIFT+SIPKSAAG
Sbjct: 482 AVIPAIDKECGEIPVAFVVKRKGSMVTKEAIINYVADQVAPYKKVRKVIFTQSIPKSAAG 541

Query: 562 KVLRRELQKHFPASKL 578
           K+LRREL K+   SKL
Sbjct: 542 KILRREL-KNSLTSKL 554

BLAST of Cucsa.149800 vs. NCBI nr
Match: gi|1000966856|ref|XP_015574447.1| (PREDICTED: LOW QUALITY PROTEIN: 4-coumarate--CoA ligase-like 6 [Ricinus communis])

HSP 1 Score: 721.5 bits (1861), Expect = 1.2e-204
Identity = 359/547 (65.63%), Postives = 437/547 (79.89%), Query Frame = 1

Query: 31  NKYPNWYSPDTGICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSISYK 90
           N  P WY P TGI  S H    LP+DPFLDVVSFIFS +  HNG +A IDS +G SI Y 
Sbjct: 15  NNNPEWYCPQTGIYTSKHPTVSLPTDPFLDVVSFIFSHK--HNGVTAFIDSFSGFSIPYS 74

Query: 91  ELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQSSSSEI 150
           +LFP+V SMASGL N+G+ QGDVVLL+LPNSI+FPI+ LG LYLGAVVTTM P S+S EI
Sbjct: 75  DLFPLVNSMASGLQNMGLKQGDVVLLLLPNSIYFPILFLGVLYLGAVVTTMNPLSTSLEI 134

Query: 151 KKRITDCNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMGFSSFYELISGGSDLI 210
           +K+I++CN  +AF++ +K    ++LGI  + VPEN   +L  +  F +FY L+ G  DL 
Sbjct: 135 EKQISNCNACIAFSVLEKAAKLQSLGIPVVSVPENVT-SLKANKAFDAFYMLLYGNVDLC 194

Query: 211 KKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAA 270
            +P+IRQEDTAAIL+SSGTTG SKGV+L+HRNFIS +ELFVRFEASQY+Y  +KNV+LA 
Sbjct: 195 LRPIIRQEDTAAILYSSGTTGSSKGVVLTHRNFISMVELFVRFEASQYDYSSSKNVFLAV 254

Query: 271 VPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILTALARTA 330
           +PMFH+YGLS+FVMGL+SLGSS+VVM KFD  + VKAID  +VTHFPVVPP+L  L + A
Sbjct: 255 LPMFHVYGLSLFVMGLLSLGSSIVVMRKFDANEXVKAIDMHEVTHFPVVPPMLQVLTKKA 314

Query: 331 EKIGVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVGTRGFNTKNA 390
           + +     +SLKQVSCGAA    KTI +FV  LP+VDFIQGYGMTESTAVGTRGFNTKN 
Sbjct: 315 KSVYGKSLKSLKQVSCGAAPLFGKTIQDFVETLPYVDFIQGYGMTESTAVGTRGFNTKNF 374

Query: 391 RNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATTFTMDQEN 450
           + Y S+GLLAPN EAKV+DWVTG  +PPG +GELL+RGP  MK YLNN EAT  T+D++ 
Sbjct: 375 QKYSSIGLLAPNIEAKVMDWVTGCFLPPGNSGELLIRGPAVMKEYLNNGEATASTIDKDG 434

Query: 451 WLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDTAVAAAKDEE 510
           WLHTGDIVY D DGYL++VDRLKE+IKYKGFQIAP DLEAV++ HP++LD AV AA D+E
Sbjct: 435 WLHTGDIVYIDHDGYLHIVDRLKEIIKYKGFQIAPADLEAVLVCHPDILDAAVTAAIDKE 494

Query: 511 CGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGKVLRRELQK 570
           CGEIPVAFVVK+  S LTQ+D+++YVA QVAP+KKVRKVIF +SIPKSAAGK+LRREL +
Sbjct: 495 CGEIPVAFVVKRLESMLTQEDIINYVAVQVAPHKKVRKVIFVQSIPKSAAGKILRREL-R 554

Query: 571 HFPASKL 578
           H+  S+L
Sbjct: 555 HYTTSRL 557

BLAST of Cucsa.149800 vs. NCBI nr
Match: gi|1009166294|ref|XP_015901516.1| (PREDICTED: 4-coumarate--CoA ligase-like 6 [Ziziphus jujuba])

HSP 1 Score: 719.9 bits (1857), Expect = 3.5e-204
Identity = 370/578 (64.01%), Postives = 457/578 (79.07%), Query Frame = 1

Query: 1   MARLINNQF-SFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFL 60
           MA  +NN F S  + E E+  +   P       +P+WYSP+TGI  S +    LPSDPFL
Sbjct: 19  MATSLNNAFTSILSEEKESEKSLHAP-------FPHWYSPETGIYSSRNPSVHLPSDPFL 78

Query: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           D VSFIFS +  HNG SALIDSS+G+SISY EL+ +V+SMASGLH +GISQGDVVL++LP
Sbjct: 79  DSVSFIFSNR--HNGVSALIDSSSGSSISYSELYRLVESMASGLHYMGISQGDVVLVLLP 138

Query: 121 NSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKT 180
           N I++PII LG LYLG VVT M P S+ SEIKK+I DCNV  AF   +K++  + LG+  
Sbjct: 139 NCIYYPIIFLGVLYLGGVVTAMNPSSTVSEIKKQIADCNVCFAFTDPEKVEKLQVLGVPA 198

Query: 181 IGVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPEN NF+  ++  FS+FY+LIS    ++ +P+I+Q DTAAI+ SSGTTGVSKGV+L+
Sbjct: 199 IGVPENVNFD-SKATCFSAFYKLISCQFSMVPRPMIKQLDTAAIMHSSGTTGVSKGVVLT 258

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFI+ +ELFVRFEASQY+Y   KNVYLA VPMFHIYGLS+F+MGL+SLGS+VVVM KF
Sbjct: 259 HRNFIAMVELFVRFEASQYDYSSLKNVYLAIVPMFHIYGLSLFIMGLLSLGSTVVVMKKF 318

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNF 360
           ++ + VKAIDR+KVTHFP+VPP+LTAL R+A+ +    FRSLKQVSCGAA  S + I+ F
Sbjct: 319 NINEAVKAIDRYKVTHFPLVPPMLTALTRSAKDVDWKSFRSLKQVSCGAAPLSWRIIEEF 378

Query: 361 VHALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           V   PHVDFIQGYG+TESTAVGTRGFNT+  RNY S+GLLAPN +AKVVD   GS +PPG
Sbjct: 379 VQTFPHVDFIQGYGLTESTAVGTRGFNTEKFRNYSSIGLLAPNMQAKVVDSNIGSFLPPG 438

Query: 421 KTGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           K+GEL LRGP  M+GYLNN EAT  ++D++ W+HTGDIVYFD DGYLY+ DRLKEVIKYK
Sbjct: 439 KSGELWLRGPAVMQGYLNNAEATMLSVDKDGWMHTGDIVYFDGDGYLYLFDRLKEVIKYK 498

Query: 481 GFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQ 540
           GFQIAP DLEAV+I HPE+ D A+A   D+E GEIPVAFVVKK  S+L++ DV+DYVA +
Sbjct: 499 GFQIAPADLEAVLICHPEIEDVAIAGTMDQESGEIPVAFVVKKHRSSLSEADVMDYVAAK 558

Query: 541 VAPYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL 578
           VAP+KKVRKV+FT SIPKSAAGK+LR++L K+  +S+L
Sbjct: 559 VAPHKKVRKVVFTASIPKSAAGKILRKDL-KNLLSSRL 585