LsiUNG000790 (gene) Bottle gourd (USVL1VR-Ls)

BLAST of LsiUNG000790 vs. Swiss-Prot
Match: 4CLL6_ARATH (4-coumarate--CoA ligase-like 6 OS=Arabidopsis thaliana GN=4CLL6 PE=2 SV=2)

HSP 1 Score: 620.5 bits (1599), Expect = 1.7e-176
Identity = 313/551 (56.81%), Postives = 412/551 (74.77%), Query Frame = 1

Query: 23  KIPRSEHKYPNWYSPDTGICRSVHASREVPSDPFLDVVSFIFSFQHNGHTAFIDSSTGNS 82
           K   S    P W+S  TGI  S   S  +P DP LD VS +FS +H+G TA IDS TG S
Sbjct: 13  KTQTSHQNPPFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFSHKHHGDTALIDSLTGFS 72

Query: 83  ISYREMFRMVKSVASGL-HKLGVSQGDVVLLVLPNSIFYPIILLGVLYLGAVITTMFPQS 142
           IS+ E+  MV+S+A+G+ H LGV QGDVV LVLPNS+++P+I L ++ LGA++TTM P S
Sbjct: 73  ISHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSS 132

Query: 143 SSSEIKKRTSECNVRLAFTIAQKINNFETLGIQAIGVPENSNLDLIRPMGFSSFHELISG 202
           S  EIKK+ SEC+V LAFT  + +    +LG+  I V E+ + D IR +    F+ ++  
Sbjct: 133 SLGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIR-IENPKFYSIMKE 192

Query: 203 GSDLNKRPVIRQEDTAAILFSSGTTGVSKGVMLTQRNFISAIELFVRFEASQYEYLPTEN 262
                 +P+I+Q+D AAI++SSGTTG SKGV+LT RN I+++ELFVRFEASQYEY  + N
Sbjct: 193 SFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSN 252

Query: 263 VYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDMVKAIDRFKVTHFPVVPPIMTS 322
           VYLAA+P+ HIYGLS+FVMGL+SLGS++VVM +FD  D+V  I+RFK+THFPVVPP++ +
Sbjct: 253 VYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLMA 312

Query: 323 MARTAEKIGVHRFRSLKQVSSGAASLNKKTIDDFVQALPHVDFIQGYGMTESTALGTRGF 382
           + + A+ +    F+SLKQVSSGAA L++K I+DF+Q LPHVD IQGYGMTESTA+GTRGF
Sbjct: 313 LTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRGF 372

Query: 383 NTKYARNYLSVGLLAPNMEAKVVDWVTGSFMPP---------------GYLNNPEATMST 442
           N++    Y SVGLLAPNM+AKVVDW +GSF+PP               GYLNNP+AT  +
Sbjct: 373 NSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQMS 432

Query: 443 IDQENWLHTGDIVYFDREGYLYVVDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDAVVAA 502
           I +++WL TGDI YFD +GYL++VDR+KE+IKYKGFQIAP DLEAV+++HP ++DA V A
Sbjct: 433 IVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTA 492

Query: 503 AKDEECGEIPVAFVIKKPGSTLSQKDVIDYVAQQVAPYKKVRKVVFTESLPKSATGKVLR 558
           A +EECGEIPVAFV+++  +TLS++DVI YVA QVAPY+KVRKVV   S+PKS TGK+LR
Sbjct: 493 APNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKILR 552

BLAST of LsiUNG000790 vs. Swiss-Prot
Match: 4CLL3_ORYSJ (4-coumarate--CoA ligase-like 3 OS=Oryza sativa subsp. japonica GN=4CLL3 PE=3 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 3.3e-135
Identity = 273/547 (49.91%), Postives = 357/547 (65.27%), Query Frame = 1

Query: 32  PNWYSPDTGICRSVHASREVPSDPFLDVVSFIFS---FQHNGHTAFIDSSTGNSISYREM 91
           P +YS  TGI  S+H    +P+DP L +V+ +F+       G    +D++T +++S  ++
Sbjct: 25  PPFYSAATGIYSSIHPPVALPTDPSLTLVAHLFARLPLADPGAPTLVDAATASAVSRADL 84

Query: 92  FRMVKSVASGLHKL-GVSQGDVVLLVLPNSIFYPIILLGVLYLGAVITTMFPQSSSSEIK 151
            R+V S+A+GL +  GV +G VVLL+LPNS+ +P+  L VL  GAV TTM P SS +EI 
Sbjct: 85  RRLVASLAAGLRRRHGVRKGSVVLLLLPNSVAFPVSFLAVLAAGAVATTMNPSSSPAEIA 144

Query: 152 KRTSECNVRLAFTIAQKINNFETLGIQAIGVPENSNLDLIRPMG------FSSFHELISG 211
            +       L             L    + VPE  +       G      F++F  ++ G
Sbjct: 145 AQARATGACLVLASRDGAARLPPLAAPVVLVPEILDHSAAADDGDDDQRVFAAFRAMLDG 204

Query: 212 GSDLNKR---PVIRQEDTAAILFSSGTTGVSKGVMLTQRNFISAIELFVRFEASQYEYLP 271
           G         PV+ Q+D  AIL+SSGT+G SKGV+LT RN I+  ELFVRFEASQY    
Sbjct: 205 GGGDGTETAVPVVGQDDAVAILYSSGTSGRSKGVVLTHRNLIAMTELFVRFEASQYHARG 264

Query: 272 T-ENVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDMVKAIDRFKVTHFPVVPP 331
             ENVY+AA+PM H+YGLS+F +GL+S+G++VVVM +FD  D V AI R+KVTH P+VPP
Sbjct: 265 ARENVYMAALPMSHVYGLSLFAVGLLSIGATVVVMRRFDAGDAVAAIGRYKVTHMPLVPP 324

Query: 332 IMTSMARTAEKIGV--HRFRSLKQVSSGAASLNKKTIDDFVQALPHVDFIQGYGMTESTA 391
           IM +M R A   GV   +  SL QVS GAA +    I +F+QA PHVDFIQGYGMTESTA
Sbjct: 325 IMAAMVRAAAAGGVPPSQVASLVQVSCGAAPITAALIHEFLQAFPHVDFIQGYGMTESTA 384

Query: 392 LGTRGFNTKYARNYLSVGLLAPNMEAKVVDWVTGSFMPPGY-----LNNP---EATMSTI 451
           +GTRGFNT   + Y SVGLLAPNM AK+V   + S +PPG+     L+ P   +  +S  
Sbjct: 385 VGTRGFNTSKHKKYTSVGLLAPNMHAKIVHLESSSCLPPGFSGELWLHGPGIMKGYLSDD 444

Query: 452 D-----QENWLHTGDIVYFDREGYLYVVDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDA 511
           D     ++ WL TGDI YFD +GYLY+V RLK+ IKYKGFQIAP DLE V++ HPE+LD 
Sbjct: 445 DDACTRKDGWLRTGDIAYFDLDGYLYIVGRLKDTIKYKGFQIAPGDLEEVLIHHPEILDV 504

Query: 512 VVAAAKDEECGEIPVAFVIKKPGSTLSQKDVIDYVAQQVAPYKKVRKVVFTESLPKSATG 550
            V +A+DEE GEIPVAFV+++ GS LS K V++YVA+QVAPYK+VRKVVF E++PKS  G
Sbjct: 505 AVTSAEDEEAGEIPVAFVVRRSGSNLSCKQVMEYVAKQVAPYKRVRKVVFVEAIPKSPAG 564

BLAST of LsiUNG000790 vs. Swiss-Prot
Match: 4CLL2_ORYSJ (4-coumarate--CoA ligase-like 2 OS=Oryza sativa subsp. japonica GN=4CLL2 PE=3 SV=3)

HSP 1 Score: 434.9 bits (1117), Expect = 1.3e-120
Identity = 245/517 (47.39%), Postives = 329/517 (63.64%), Query Frame = 1

Query: 29  HKYPNWYSPDTGICRSVHASREVPSDPFLDVVSFIFS-FQHNGHTAFIDSSTGNSISYRE 88
           H   ++YS  TG+  S+H    +PSDP L +V  +FS    + H+  +D+ T  ++S  +
Sbjct: 10  HPSLSFYSAATGLYSSLHPPLPLPSDPSLSLVPHLFSHLPLHHHSLLVDAPTAATLSCAD 69

Query: 89  MFRMVKSVASGLHK-LGVSQGDVVLLVLPNSIFYPIILLGVLYLGAVITTMFPQSSSSEI 148
             R+V S+A+GL + L +++G +VLL+LPNS+ +P+  L VL  GAV TTM P S+ +EI
Sbjct: 70  FRRLVSSLAAGLRRRLHIARGSLVLLLLPNSLTFPVAFLAVLATGAVATTMNPSSAPAEI 129

Query: 149 KKRTSECNVRLAFTIAQKINNFETLGIQAIGVPENSNLDLIRPMGFSSFHELISGGSD-- 208
             R  +    L             L    + VP+              FH L+    +  
Sbjct: 130 AARLRDTAPSLVLASTHNAAKLPPLAAPLVLVPDTFQQQHDDDQFDFFFHALLETDPETP 189

Query: 209 LNKRPVIRQEDTAAILFSSGTTGVSKGVMLTQRNFISAIELFVRFEASQYEYLPTENVYL 268
           +     + Q+D AA+L+SSGT+G SKGV++T RN I+ +ELFVRFEASQY     +NVYL
Sbjct: 190 VEMGVGVGQDDAAAVLYSSGTSGRSKGVVVTHRNLIAMVELFVRFEASQYTRPARDNVYL 249

Query: 269 AAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDMVKAIDRFKVTHFPVVPPIMTSMAR 328
           AA+PMFH+YGLS+F +GL+SLG +VVVM +F+V D VKAI ++KVTH P+VPPIM+++ R
Sbjct: 250 AALPMFHVYGLSLFAVGLLSLGCTVVVMRRFNVDDAVKAIRKYKVTHLPLVPPIMSALLR 309

Query: 329 TAEKIGVHRFRSLKQVSSGAASLNKKTIDDFVQALPHVDFIQGYGMTESTALGTRGFNTK 388
               +      SL QVSSGAA LN   I  F+ A PHVDFIQGYGMTESTA+GTRGFNT 
Sbjct: 310 ANPPL---ELDSLLQVSSGAAPLNHTLIHHFLHAFPHVDFIQGYGMTESTAVGTRGFNTC 369

Query: 389 YARNYLSVGLLAPNMEAKVVDWVTGSFMPPG-----YLNNPEATMSTI------------ 448
             + Y SVGLLAPNM AK+V   +GS +PPG     +L+ P A M               
Sbjct: 370 KHKKYASVGLLAPNMHAKIVHLESGSCLPPGSYGELWLHGP-AIMKEFCFVTGYLNDDDD 429

Query: 449 ---DQENWLHTGDIVYFDREGYLYVVDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDAVV 508
               ++ WL TGDI YFD +GYL++V RLK+ IKYKGFQIAP DLEAV++ HPE++D  V
Sbjct: 430 AFTRKDGWLRTGDIAYFDSDGYLFIVGRLKDTIKYKGFQIAPADLEAVLIRHPEIIDVAV 489

Query: 509 AAAKDEECGEIPVAFVIKKPGSTLSQKDVIDYVAQQV 522
            + +DEE GEIPVAFV++K GSTLS   V++YVA+QV
Sbjct: 490 TSDEDEEAGEIPVAFVVRKSGSTLSCTHVMEYVAKQV 522

BLAST of LsiUNG000790 vs. Swiss-Prot
Match: 4CLL5_ARATH (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 413.7 bits (1062), Expect = 3.2e-114
Identity = 214/519 (41.23%), Postives = 337/519 (64.93%), Query Frame = 1

Query: 51  VPSDPFLDVVSFIFSFQHNGHTAFIDSSTGNSISYREMFRMVKSVASGLHKLGVSQGDVV 110
           +P +P LDV +FI S  H G  AFID+STG ++++ E++R V+SVA  L ++G+ +G VV
Sbjct: 26  LPPNPSLDVTTFISSQAHRGRIAFIDASTGQNLTFTELWRAVESVADCLSEIGIRKGHVV 85

Query: 111 LLVLPNSIFYPIILLGVLYLGAVITTMFPQSSSSEIKKRTSECNVRLAFTIAQKINNFET 170
           LL+ PNSI +P++ L V+ LGA+ITT  P ++S+EI K+  + N  LAFT +Q +     
Sbjct: 86  LLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISA 145

Query: 171 LGIQA-IGVPENSNLDLIRPMG-FSSFHELISGGSDLNKRPVIRQEDTAAILFSSGTTGV 230
              +  I + +   +D +  +       +    G+ + +R  + Q+DTA +L+SSGTTG+
Sbjct: 146 AAKKLPIVLMDEERVDSVGDVRRLVEMMKKEPSGNRVKER--VDQDDTATLLYSSGTTGM 205

Query: 231 SKGVMLTQRNFISAIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLMSLGSS 290
           SKGV+ + RN I+ ++  V    S       E  ++  +PMFHIYGL+ F  GL++ GS+
Sbjct: 206 SKGVISSHRNLIAMVQTIVNRFGSD----DGEQRFICTVPMFHIYGLAAFATGLLAYGST 265

Query: 291 VVVMSKFDVKDMVKAIDRFKVTHFPVVPPIMTSMARTAEKIGV-HRFRSLKQVSSGAASL 350
           ++V+SKF++ +M+ AI +++ T  P+VPPI+ +M   A++I   +   S+  V  G A L
Sbjct: 266 IIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSSMHTVLCGGAPL 325

Query: 351 NKKTIDDFVQALPHVDFIQGYGMTESTALGTRGFNTKYARNYLSVGLLAPNMEAKVVDWV 410
           +K+  + F +  P V  +QGYG+TEST +G      + +R Y + G L+ +ME ++VD V
Sbjct: 326 SKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRIVDPV 385

Query: 411 TGSFMPP---------------GYLNNPEATMSTIDQENWLHTGDIVYFDREGYLYVVDR 470
           TG  + P               GY +N EAT ST+D E WL TGD+ Y D +G+++VVDR
Sbjct: 386 TGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVVDR 445

Query: 471 LKEVIKYKGFQIAPTDLEAVVMTHPEVLDAVVAAAKDEECGEIPVAFVIKKPGSTLSQKD 530
           LKE+IKYKG+Q+AP +LEA+++THPE+ DA V    D+E G+ P+A+V++K GS+LS+K 
Sbjct: 446 LKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKT 505

Query: 531 VIDYVAQQVAPYKKVRKVVFTESLPKSATGKVLRRELGK 552
           ++++VA+QVAPYK++RKV F  S+PK+ +GK+LR++L K
Sbjct: 506 IMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLIK 538

BLAST of LsiUNG000790 vs. Swiss-Prot
Match: 4CLL4_ORYSJ (4-coumarate--CoA ligase-like 4 OS=Oryza sativa subsp. japonica GN=4CLL4 PE=2 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 4.6e-113
Identity = 220/554 (39.71%), Postives = 345/554 (62.27%), Query Frame = 1

Query: 25  PRSEHKYPNWYSPDTGICRSVHASREVPSDPFLDVVSFIFSFQHNGHTAFIDSSTGNSIS 84
           P  E    + Y   T   RS  A   +P+DP +DVVSF+ S +H+G  A +D++TG  I+
Sbjct: 5   PEMEVDARSGYCAATRTFRSRRADVPLPADPEVDVVSFLASRRHSGVVALVDAATGRRIT 64

Query: 85  YREMFRMVKSVASGL--HKLGVSQGDVVLLVLPNSIFYPIILLGVLYLGAVITTMFPQSS 144
           + E++R V   AS L  H + + +G V L++ PNS+ +P+  L  + LGAV+TT  P ++
Sbjct: 65  FTELWRAVAGAASALAAHPVSLRKGHVALILSPNSVHFPVAALAAMSLGAVLTTANPLNT 124

Query: 145 SSEIKKRTSECNVRLAFTIAQKINNFETLGIQAIGVPENSNL--DLIRPMGFSSFHELIS 204
            +EI K+ ++    LAFT  + +          + + E++ L  D   P   ++  E+ +
Sbjct: 125 PAEIAKQVADARPVLAFTTRELLPKLPRAHDLRVVLLESARLPGDSSDPRIVATIEEISA 184

Query: 205 GGSD-LNKRPVIRQEDTAAILFSSGTTGVSKGVMLTQRNFISAIELFV-RFEASQYEYLP 264
              D   ++  + Q+D A +L+SSGTTG SKGV+ T R+ IS +++ + RF     +   
Sbjct: 185 TTPDPARRKDRVTQDDPATLLYSSGTTGPSKGVVATHRSLISMVQIIMTRFRLEGSDKTE 244

Query: 265 TENVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDMVKAIDRFKVTHFPVVPPI 324
           T   +L  +PMFH+YGL  F  GL+  G++VVV+SK+++ +M+++I+ + VT+ P+VPPI
Sbjct: 245 T---FLCTVPMFHVYGLVAFATGLLGCGATVVVLSKYELPEMLRSINAYGVTYLPLVPPI 304

Query: 325 MTSMARTAEKIGVHRFRSLKQVSSGAASLNKKTIDDFVQALPHVDFIQGYGMTESTALGT 384
           + +M    + + + + R   +V SG A L K+ I+ F +  P V+ +QGYG+TESTA+G 
Sbjct: 305 LVAMVAHPKPLPLGQMR---KVLSGGAPLGKELIEGFREKYPQVEILQGYGLTESTAIGA 364

Query: 385 RGFNTKYARNYLSVGLLAPNMEAKVVD---------------WVTGSFMPPGYLNNPEAT 444
              + + +R Y + GLL+PN EAK+VD               W+ G ++  GY  N EAT
Sbjct: 365 STDSAEESRRYGTAGLLSPNTEAKIVDPDSGEALPVNRTGELWIRGPYVMKGYFKNAEAT 424

Query: 445 MSTIDQENWLHTGDIVYFDREGYLYVVDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDAV 504
            ST+  + WL TGD+ Y D +GYL+VVDRLKE+IKYKG+Q+ P +LEA+++THPEV D  
Sbjct: 425 QSTLTPDGWLKTGDLCYIDEDGYLFVVDRLKELIKYKGYQVPPAELEALLLTHPEVTDVA 484

Query: 505 VAAAKDEECGEIPVAFVIKKPGSTLSQKDVIDYVAQQVAPYKKVRKVVFTESLPKSATGK 558
           V    D E G+ P+A++++K GS LS+++V+++VA+QVAPYKKVRKV F   +PK+A+GK
Sbjct: 485 VIPFPDREVGQFPMAYIVRKKGSNLSEREVMEFVAKQVAPYKKVRKVAFVTDIPKNASGK 544

BLAST of LsiUNG000790 vs. TrEMBL
Match: A0A0A0L8X1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G638510 PE=4 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 2.1e-274
Identity = 480/574 (83.62%), Postives = 523/574 (91.11%), Query Frame = 1

Query: 1   MARLLNDHFSFQTPENEAVITKKIPRSE--HKYPNWYSPDTGICRSVHASREVPSDPFLD 60
           MARL+N+ FSF+TPENEAVITKKIPRSE  +KYPNWYSPDTGICRSVH  R++PSDPFLD
Sbjct: 1   MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD 60

Query: 61  VVSFIFSFQ--HNGHTAFIDSSTGNSISYREMFRMVKSVASGLHKLGVSQGDVVLLVLPN 120
           VVSFIFSFQ  HNGH+A IDSSTGNSISY+E+F MVKS+ASGLH LG+SQGDVVLL+LPN
Sbjct: 61  VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN 120

Query: 121 SIFYPIILLGVLYLGAVITTMFPQSSSSEIKKRTSECNVRLAFTIAQKINNFETLGIQAI 180
           SIF+PII+LG LYLGAV+TTMFPQSSSSEIKKR ++CNVRLAF I QKI NFE LGI+ I
Sbjct: 121 SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI 180

Query: 181 GVPENSNLDLIRPMGFSSFHELISGGSDLNKRPVIRQEDTAAILFSSGTTGVSKGVMLTQ 240
           GVPEN+N +L+R MGFSSF+ELISGGSDL K+PVIRQEDTAAILFSSGTTGVSKGVML+ 
Sbjct: 181 GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH 240

Query: 241 RNFISAIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFD 300
           RNFIS IELFVRFEASQYEYLPT+NVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKFD
Sbjct: 241 RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD 300

Query: 301 VKDMVKAIDRFKVTHFPVVPPIMTSMARTAEKIGVHRFRSLKQVSSGAASLNKKTIDDFV 360
           VKD+VKAIDRFKVTHFPVVPPI+T++ARTAEKIGVHRFRSLKQVS GAA+ +KKTID+FV
Sbjct: 301 VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV 360

Query: 361 QALPHVDFIQGYGMTESTALGTRGFNTKYARNYLSVGLLAPNMEAKVVDWVTGSFMPP-- 420
            ALPHVDFIQGYGMTESTA+GTRGFNTK ARNYLSVGLLAPN EAKVVDWVTGS MPP  
Sbjct: 361 HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK 420

Query: 421 -------------GYLNNPEATMSTIDQENWLHTGDIVYFDREGYLYVVDRLKEVIKYKG 480
                        GYLNNPEAT  T+DQENWLHTGDIVYFDR+GYLYVVDRLKEVIKYKG
Sbjct: 421 TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG 480

Query: 481 FQIAPTDLEAVVMTHPEVLDAVVAAAKDEECGEIPVAFVIKKPGSTLSQKDVIDYVAQQV 540
           FQIAPTDLEAVV+THPEVLD  VAAAKDEECGEIPVAFV+KKPGS L+QKDV+DYVAQQV
Sbjct: 481 FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV 540

Query: 541 APYKKVRKVVFTESLPKSATGKVLRRELGKHLPS 556
           APYKKVRKV+FTES+PKSA GKVLRREL KH P+
Sbjct: 541 APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPA 574

BLAST of LsiUNG000790 vs. TrEMBL
Match: A0A061G6U1_THECC (Acyl:coa ligase OS=Theobroma cacao GN=TCM_016640 PE=4 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 4.4e-195
Identity = 350/572 (61.19%), Postives = 441/572 (77.10%), Query Frame = 1

Query: 1   MARLLNDHFSFQTPENEAVITKKIPRSEHKYPNWYSPDTGICRSVHASREVPSDPFLDVV 60
           MA  LN HFSFQ  E++            ++P+WYSP+TGI  S H+S  +P+D FLD+V
Sbjct: 1   MATSLNSHFSFQRSESKTT----------RFPDWYSPETGIYSSKHSSISLPTDHFLDIV 60

Query: 61  SFIFSFQHNGHTAFIDSSTGNSISYREMFRMVKSVASGLHKLGVSQGDVVLLVLPNSIFY 120
           SFIFS QH+G TA IDSS+G S SY ++  +++S+ASGLH LGVSQGDVVLL+LPNS+ +
Sbjct: 61  SFIFSHQHDGVTALIDSSSGYSTSYAKLLPLIQSMASGLHHLGVSQGDVVLLLLPNSVHF 120

Query: 121 PIILLGVLYLGAVITTMFPQSSSSEIKKRTSECNVRLAFTIAQKINNFETLGIQAIGVPE 180
           PII L VLYLGA++TTM P +S  EIKK+ ++ +VR AFT+  K++  + LG+ AIGVPE
Sbjct: 121 PIIFLSVLYLGAIVTTMNPLTSMLEIKKQIADSSVRFAFTLLDKVDKLKNLGVHAIGVPE 180

Query: 181 NSNLDLIRPMGFSSFHELISGGSDLNKRPVIRQEDTAAILFSSGTTGVSKGVMLTQRNFI 240
           N NLD  +   F  FH+LI G  D   RPVIRQ+DTAAI++SSGTTGVSKGV+LT  NFI
Sbjct: 181 NLNLDSEKT-DFLLFHKLIGGQFDKAPRPVIRQQDTAAIMYSSGTTGVSKGVVLTHGNFI 240

Query: 241 SAIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDM 300
           + +ELFVRFEASQYEY  ++NVYLA +PMFHIYGLS+FV+GL+SLG+S+VVM +F+  +M
Sbjct: 241 AMVELFVRFEASQYEYSSSKNVYLAVLPMFHIYGLSLFVVGLLSLGTSIVVMRRFNATEM 300

Query: 301 VKAIDRFKVTHFPVVPPIMTSMARTAEKIGVHRFRSLKQVSSGAASLNKKTIDDFVQALP 360
           V+ ID++ VTHFPVVPPI+ ++A  A+ +  +  +SLKQVS GAA L++K I+DFVQALP
Sbjct: 301 VRVIDKYGVTHFPVVPPILRTLAMRAKGVCENSLKSLKQVSCGAAPLSRKAIEDFVQALP 360

Query: 361 HVDFIQGYGMTESTALGTRGFNTKYARNYLSVGLLAPNMEAKVVDWVTGSFMPP------ 420
           HVDFIQGYGMTESTA+G RGFN +    Y SVGLLAPNM+AKVVDW +GS +PP      
Sbjct: 361 HVDFIQGYGMTESTAVGNRGFNNEKHHKYSSVGLLAPNMQAKVVDWNSGSSLPPDSHGEL 420

Query: 421 ---------GYLNNPEATMSTIDQENWLHTGDIVYFDREGYLYVVDRLKEVIKYKGFQIA 480
                    GYLNN EAT  TID++ WL TGDIV FD +GY+YV DRLKE+IKYKG+QIA
Sbjct: 421 WLRGPGLMQGYLNNVEATRMTIDKDGWLRTGDIVCFDEDGYMYVSDRLKEIIKYKGYQIA 480

Query: 481 PTDLEAVVMTHPEVLDAVVAAAKDEECGEIPVAFVIKKPGSTLSQKDVIDYVAQQVAPYK 540
           P DLEA++++HPE+LDA V  A DEE GEIPVAFV+++ GSTL+Q  V+D+VA+QVAPYK
Sbjct: 481 PADLEAILISHPEILDAAVIPAIDEEAGEIPVAFVVRRQGSTLTQGAVMDFVAKQVAPYK 540

Query: 541 KVRKVVFTESLPKSATGKVLRRELGKHLPSKL 558
           KVRKVVFT+S+PKSA GK LRREL   L S+L
Sbjct: 541 KVRKVVFTKSIPKSAAGKTLRRELRNFLSSRL 561

BLAST of LsiUNG000790 vs. TrEMBL
Match: W9SFR0_9ROSA (4-coumarate--CoA ligase-like 6 OS=Morus notabilis GN=L484_022816 PE=4 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 1.9e-193
Identity = 341/544 (62.68%), Postives = 430/544 (79.04%), Query Frame = 1

Query: 30  KYPNWYSPDTGICRSVHASREVPSDPFLDVVSFIFSFQHNGHTAFIDSSTGNS-ISYREM 89
           ++P+WYSP+TGIC S +     PSDPFLDVVSFIFS QHNG +A +DSS+G+S ISY E+
Sbjct: 12  QFPHWYSPETGICHSTNRPITFPSDPFLDVVSFIFSKQHNGVSALVDSSSGSSSISYSEL 71

Query: 90  FRMVKSVASGLHKLGVSQGDVVLLVLPNSIFYPIILLGVLYLGAVITTMFPQSSSSEIKK 149
            R+VKS+ASGLH +GVS+ DVVLL+LPNS+++P+ILLGV+YLGA++TTM P S+ +E+KK
Sbjct: 72  HRLVKSMASGLHSMGVSKNDVVLLLLPNSVYFPVILLGVVYLGAIVTTMNPLSTVTEVKK 131

Query: 150 RTSECNVRLAFTIAQKINNFETLGIQAIGVPENSNLDLIRPMGFSSFHELISGGSDLNKR 209
           + ++    LAFT+ +K+       + AI VPEN N        F  F++L+S   D   R
Sbjct: 132 QVADSKACLAFTLPEKVELLRAFRVGAIAVPENFNSSQKPMSSFREFYKLVSCEFDFAPR 191

Query: 210 PVIRQEDTAAILFSSGTTGVSKGVMLTQRNFISAIELFVRFEASQYEYLPTENVYLAAIP 269
           P+IRQ+DTAAI++SSGTTG SKGV+LT  NFI+ ++LFVRFEASQYEY  +ENVYLA +P
Sbjct: 192 PLIRQQDTAAIMYSSGTTGASKGVVLTHGNFIAMVDLFVRFEASQYEYPRSENVYLAVLP 251

Query: 270 MFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDMVKAIDRFKVTHFPVVPPIMTSMARTAEK 329
           MFHIYGL++FVMGL+SLGS++VVM KFDV DMVK I+ +KVTHFPVVPP++ ++ + A+ 
Sbjct: 252 MFHIYGLALFVMGLLSLGSTIVVMRKFDVNDMVKTIETYKVTHFPVVPPMLIALTKCAKV 311

Query: 330 IGVHRFRSLKQVSSGAASLNKKTIDDFVQALPHVDFIQGYGMTESTALGTRGFNTKYARN 389
           +G   FRSLKQVSSGAA ++ K I+DF+  LPHVDFIQGYGMTESTA+GTRGFNTK  R 
Sbjct: 312 VGGKGFRSLKQVSSGAAPISGKIIEDFLHTLPHVDFIQGYGMTESTAVGTRGFNTKKCRK 371

Query: 390 YLSVGLLAPNMEAKVVDWVTGSFMPP---------------GYLNNPEATMSTIDQENWL 449
           + SVGLL PNMEAKV+D  TGSF+PP               GYLNN  ATMS ID++ WL
Sbjct: 372 FSSVGLLGPNMEAKVMDLNTGSFLPPGSCGELWLRGPAVMKGYLNNAVATMSAIDKDGWL 431

Query: 450 HTGDIVYFDREGYLYVVDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDAVVAAAKDEECG 509
            TGDIVYFD++GYL+VVDRLKE+IKYKG+QIAP DLEAV+ +HPE+ D  VAAA+D + G
Sbjct: 432 RTGDIVYFDQDGYLHVVDRLKEIIKYKGYQIAPADLEAVLASHPEIRDVAVAAAEDADSG 491

Query: 510 EIPVAFVIKKPGSTLSQKDVIDYVAQQVAPYKKVRKVVFTESLPKSATGKVLRRELGKHL 558
           E+PVAFV++K GS+LSQ+ V+DYVA+QVAPYKK+RKVVFT S+PKSA GK+LRREL K L
Sbjct: 492 EVPVAFVVRKHGSSLSQEAVMDYVARQVAPYKKIRKVVFTHSIPKSAAGKILRRELQKSL 551

BLAST of LsiUNG000790 vs. TrEMBL
Match: A0A0D2S1Q6_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_004G180900 PE=4 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 1.0e-191
Identity = 343/548 (62.59%), Postives = 430/548 (78.47%), Query Frame = 1

Query: 25  PRSEHKYPNWYSPDTGICRSVHASREVPSDPFLDVVSFIFSFQHNGHTAFIDSSTGNSIS 84
           P++ H  P+WYSP+T I  S ++S  +P+DPFLD+VSFIFS QH+G TA IDSS+G SIS
Sbjct: 14  PKTTH-LPDWYSPETRIYSSTYSSISLPTDPFLDIVSFIFSHQHDGVTALIDSSSGYSIS 73

Query: 85  YREMFRMVKSVASGLHKLGVSQGDVVLLVLPNSIFYPIILLGVLYLGAVITTMFPQSSSS 144
           Y ++  +V+S+ASGLH LGVSQGDVVLL+LPNS+ +PII   VLYLGAV+TTM P S+  
Sbjct: 74  YSKLLPLVQSMASGLHHLGVSQGDVVLLLLPNSVNFPIIFFSVLYLGAVVTTMNPLSNMM 133

Query: 145 EIKKRTSECNVRLAFTIAQKINNFETLGIQAIGVPENSNLDLIRPMGFSSFHELISGGSD 204
           E+KK+  +C VR AFT+ + ++  + LG+ AIGVPEN +LD  +  GF SF +LI G   
Sbjct: 134 EVKKQIVDCGVRFAFTLLENVDKLQKLGVHAIGVPENMDLDSEKT-GFLSFRKLIGGQFG 193

Query: 205 LNKRPVIRQEDTAAILFSSGTTGVSKGVMLTQRNFISAIELFVRFEASQYEYLPTENVYL 264
              RPVIRQ+DTAAI++SSGTTGVSKGV+LT  NFI+  ELFVRFEASQYEY  ++NVYL
Sbjct: 194 KAPRPVIRQQDTAAIMYSSGTTGVSKGVVLTHGNFIATTELFVRFEASQYEYSSSKNVYL 253

Query: 265 AAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDMVKAIDRFKVTHFPVVPPIMTSMAR 324
           A +PMFHIYGLS+FV+GL+SLGSS+V+M +FD  ++VK ID + VTHFPVVPPI+T++  
Sbjct: 254 AVLPMFHIYGLSLFVVGLLSLGSSIVIMRRFDASELVKVIDDYGVTHFPVVPPILTTLTM 313

Query: 325 TAEKIGVHRFRSLKQVSSGAASLNKKTIDDFVQALPHVDFIQGYGMTESTALGTRGFNTK 384
            A+ +  +  +SLKQVS GAA L+ KTI+DFVQALPHVDFIQGYGMTESTA+GTRGFN +
Sbjct: 314 RAKHVCENSLQSLKQVSCGAAPLSGKTIEDFVQALPHVDFIQGYGMTESTAVGTRGFNNE 373

Query: 385 YARNYLSVGLLAPNMEAKVVDWVTGSFMPPG---------------YLNNPEATMSTIDQ 444
              NY S+GLLAPNM+AKVVDW +GS +PPG               Y+NN EAT  TID+
Sbjct: 374 KHHNYSSIGLLAPNMQAKVVDWNSGSSLPPGFHGELWLRGPAIMQRYINNVEATNMTIDK 433

Query: 445 ENWLHTGDIVYFDREGYLYVVDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDAVVAAAKD 504
           + WL TGDIV FD EGYLY+ DRLKE+IKYKG+QIAP DLEAV+++HPE+LDA V +A D
Sbjct: 434 DGWLRTGDIVCFDGEGYLYLSDRLKEIIKYKGYQIAPADLEAVLISHPEILDAAVTSAAD 493

Query: 505 EECGEIPVAFVIKKPGSTLSQKDVIDYVAQQVAPYKKVRKVVFTESLPKSATGKVLRREL 558
           E CGEIPVAFV+++ GSTL++  VID+VA QVAPYKKVR+VVFTES+PKSA GK LR+EL
Sbjct: 494 EVCGEIPVAFVVRRHGSTLTKGAVIDFVANQVAPYKKVRRVVFTESIPKSAAGKNLRKEL 553

BLAST of LsiUNG000790 vs. TrEMBL
Match: S4W608_CHOAR (Ca4CL9 OS=Chosenia arbutifolia PE=2 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 2.3e-191
Identity = 340/547 (62.16%), Postives = 426/547 (77.88%), Query Frame = 1

Query: 27  SEHKYPNWYSPDTGICRSVHASREVPSDPFLDVVSFIFSFQ-HNGHTAFIDSSTGNSISY 86
           S+  +P+WYSP+TGI  S H +  +P+DPFLDVVSFIFS   HNG TA IDSS+G SISY
Sbjct: 9   SKTTFPDWYSPETGIYSSKHPTIPLPADPFLDVVSFIFSHHNHNGLTALIDSSSGFSISY 68

Query: 87  REMFRMVKSVASGLHKLGVSQGDVVLLVLPNSIFYPIILLGVLYLGAVITTMFPQSSSSE 146
            ++  +VKSVASGL  +G+ QGDVVLL+LPNSI +PI+ LGVLYLG +++TM P SS  E
Sbjct: 69  SKILPLVKSVASGLRNMGIKQGDVVLLLLPNSIHFPIVFLGVLYLGGIVSTMNPLSSEPE 128

Query: 147 IKKRTSECNVRLAFTIAQKINNFETLGIQAIGVPENSNLDLIRPMGFSSFHELISGGSDL 206
           +KKR ++CN R+AF   +K+  F+ LGI  IGV EN NLD  +   F +F+  +    D+
Sbjct: 129 VKKRIADCNARIAFVELKKVGKFQPLGILVIGVQENVNLDE-KSNDFEAFYMRLYSKGDV 188

Query: 207 NKRPVIRQEDTAAILFSSGTTGVSKGVMLTQRNFISAIELFVRFEASQYEYLPTENVYLA 266
             +PVIRQ+DTAAI++SSGTT  SKGV+LT RNFIS +ELFV+FEASQYEY  T+NVYLA
Sbjct: 189 GVKPVIRQQDTAAIMYSSGTTAASKGVVLTHRNFISMVELFVKFEASQYEYSSTDNVYLA 248

Query: 267 AIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDMVKAIDRFKVTHFPVVPPIMTSMART 326
            +PMFHIYGLS+FV+GL+SLGSS+VVM KFDV + VK IDR+ VTHFPVVPPI+T++ RT
Sbjct: 249 VLPMFHIYGLSLFVVGLLSLGSSIVVMRKFDVSETVKVIDRYGVTHFPVVPPILTALTRT 308

Query: 327 AEKIGVHRFRSLKQVSSGAASLNKKTIDDFVQALPHVDFIQGYGMTESTALGTRGFNTKY 386
           A+ +  +  + LK VS GAA L  KTI DFV+ LPHVDFIQGYG+TESTA+GTRG NT+ 
Sbjct: 309 AKGVCGNSLKCLKVVSCGAAPLFAKTIQDFVEVLPHVDFIQGYGLTESTAVGTRGLNTEK 368

Query: 387 ARNYLSVGLLAPNMEAKVVDWVTGSFMPPG---------------YLNNPEATMSTIDQE 446
            + Y S+GLLAPN+EAKVVDW+TG+ +PPG               YLN+ EAT  TID++
Sbjct: 369 FKKYSSIGLLAPNIEAKVVDWITGTLLPPGGSGELWIRGPGVMKEYLNDGEATALTIDKD 428

Query: 447 NWLHTGDIVYFDREGYLYVVDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDAVVAAAKDE 506
            WLHTGD+VY D +GYLYVVDRLKE+IKYKGFQIAP DLEAV+++H E+LDA V  A D+
Sbjct: 429 GWLHTGDVVYTDHDGYLYVVDRLKEIIKYKGFQIAPADLEAVLISHCEILDAAVIPAIDK 488

Query: 507 ECGEIPVAFVIKKPGSTLSQKDVIDYVAQQVAPYKKVRKVVFTESLPKSATGKVLRRELG 558
           ECGEIPVAFV+K+ GS ++++ +I+YVA QVAPYKKVRKV+FT+S+PKSA GK+LRREL 
Sbjct: 489 ECGEIPVAFVVKRKGSMVTKEAIINYVADQVAPYKKVRKVIFTQSIPKSAAGKILRRELK 548

BLAST of LsiUNG000790 vs. TAIR10
Match: AT4G19010.1 (AT4G19010.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 620.5 bits (1599), Expect = 9.8e-178
Identity = 313/551 (56.81%), Postives = 412/551 (74.77%), Query Frame = 1

Query: 23  KIPRSEHKYPNWYSPDTGICRSVHASREVPSDPFLDVVSFIFSFQHNGHTAFIDSSTGNS 82
           K   S    P W+S  TGI  S   S  +P DP LD VS +FS +H+G TA IDS TG S
Sbjct: 13  KTQTSHQNPPFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFSHKHHGDTALIDSLTGFS 72

Query: 83  ISYREMFRMVKSVASGL-HKLGVSQGDVVLLVLPNSIFYPIILLGVLYLGAVITTMFPQS 142
           IS+ E+  MV+S+A+G+ H LGV QGDVV LVLPNS+++P+I L ++ LGA++TTM P S
Sbjct: 73  ISHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSS 132

Query: 143 SSSEIKKRTSECNVRLAFTIAQKINNFETLGIQAIGVPENSNLDLIRPMGFSSFHELISG 202
           S  EIKK+ SEC+V LAFT  + +    +LG+  I V E+ + D IR +    F+ ++  
Sbjct: 133 SLGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIR-IENPKFYSIMKE 192

Query: 203 GSDLNKRPVIRQEDTAAILFSSGTTGVSKGVMLTQRNFISAIELFVRFEASQYEYLPTEN 262
                 +P+I+Q+D AAI++SSGTTG SKGV+LT RN I+++ELFVRFEASQYEY  + N
Sbjct: 193 SFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSN 252

Query: 263 VYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDMVKAIDRFKVTHFPVVPPIMTS 322
           VYLAA+P+ HIYGLS+FVMGL+SLGS++VVM +FD  D+V  I+RFK+THFPVVPP++ +
Sbjct: 253 VYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLMA 312

Query: 323 MARTAEKIGVHRFRSLKQVSSGAASLNKKTIDDFVQALPHVDFIQGYGMTESTALGTRGF 382
           + + A+ +    F+SLKQVSSGAA L++K I+DF+Q LPHVD IQGYGMTESTA+GTRGF
Sbjct: 313 LTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRGF 372

Query: 383 NTKYARNYLSVGLLAPNMEAKVVDWVTGSFMPP---------------GYLNNPEATMST 442
           N++    Y SVGLLAPNM+AKVVDW +GSF+PP               GYLNNP+AT  +
Sbjct: 373 NSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQMS 432

Query: 443 IDQENWLHTGDIVYFDREGYLYVVDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDAVVAA 502
           I +++WL TGDI YFD +GYL++VDR+KE+IKYKGFQIAP DLEAV+++HP ++DA V A
Sbjct: 433 IVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTA 492

Query: 503 AKDEECGEIPVAFVIKKPGSTLSQKDVIDYVAQQVAPYKKVRKVVFTESLPKSATGKVLR 558
           A +EECGEIPVAFV+++  +TLS++DVI YVA QVAPY+KVRKVV   S+PKS TGK+LR
Sbjct: 493 APNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKILR 552

BLAST of LsiUNG000790 vs. TAIR10
Match: AT1G20510.1 (AT1G20510.1 OPC-8:0 CoA ligase1)

HSP 1 Score: 413.7 bits (1062), Expect = 1.8e-115
Identity = 214/519 (41.23%), Postives = 337/519 (64.93%), Query Frame = 1

Query: 51  VPSDPFLDVVSFIFSFQHNGHTAFIDSSTGNSISYREMFRMVKSVASGLHKLGVSQGDVV 110
           +P +P LDV +FI S  H G  AFID+STG ++++ E++R V+SVA  L ++G+ +G VV
Sbjct: 26  LPPNPSLDVTTFISSQAHRGRIAFIDASTGQNLTFTELWRAVESVADCLSEIGIRKGHVV 85

Query: 111 LLVLPNSIFYPIILLGVLYLGAVITTMFPQSSSSEIKKRTSECNVRLAFTIAQKINNFET 170
           LL+ PNSI +P++ L V+ LGA+ITT  P ++S+EI K+  + N  LAFT +Q +     
Sbjct: 86  LLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISA 145

Query: 171 LGIQA-IGVPENSNLDLIRPMG-FSSFHELISGGSDLNKRPVIRQEDTAAILFSSGTTGV 230
              +  I + +   +D +  +       +    G+ + +R  + Q+DTA +L+SSGTTG+
Sbjct: 146 AAKKLPIVLMDEERVDSVGDVRRLVEMMKKEPSGNRVKER--VDQDDTATLLYSSGTTGM 205

Query: 231 SKGVMLTQRNFISAIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLMSLGSS 290
           SKGV+ + RN I+ ++  V    S       E  ++  +PMFHIYGL+ F  GL++ GS+
Sbjct: 206 SKGVISSHRNLIAMVQTIVNRFGSD----DGEQRFICTVPMFHIYGLAAFATGLLAYGST 265

Query: 291 VVVMSKFDVKDMVKAIDRFKVTHFPVVPPIMTSMARTAEKIGV-HRFRSLKQVSSGAASL 350
           ++V+SKF++ +M+ AI +++ T  P+VPPI+ +M   A++I   +   S+  V  G A L
Sbjct: 266 IIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSSMHTVLCGGAPL 325

Query: 351 NKKTIDDFVQALPHVDFIQGYGMTESTALGTRGFNTKYARNYLSVGLLAPNMEAKVVDWV 410
           +K+  + F +  P V  +QGYG+TEST +G      + +R Y + G L+ +ME ++VD V
Sbjct: 326 SKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRIVDPV 385

Query: 411 TGSFMPP---------------GYLNNPEATMSTIDQENWLHTGDIVYFDREGYLYVVDR 470
           TG  + P               GY +N EAT ST+D E WL TGD+ Y D +G+++VVDR
Sbjct: 386 TGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVVDR 445

Query: 471 LKEVIKYKGFQIAPTDLEAVVMTHPEVLDAVVAAAKDEECGEIPVAFVIKKPGSTLSQKD 530
           LKE+IKYKG+Q+AP +LEA+++THPE+ DA V    D+E G+ P+A+V++K GS+LS+K 
Sbjct: 446 LKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKT 505

Query: 531 VIDYVAQQVAPYKKVRKVVFTESLPKSATGKVLRRELGK 552
           ++++VA+QVAPYK++RKV F  S+PK+ +GK+LR++L K
Sbjct: 506 IMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLIK 538

BLAST of LsiUNG000790 vs. TAIR10
Match: AT1G20480.1 (AT1G20480.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 397.5 bits (1020), Expect = 1.3e-110
Identity = 222/551 (40.29%), Postives = 336/551 (60.98%), Query Frame = 1

Query: 39  TGICRSV---HASRE---VPSDPFLDVVSFIFSFQHNGHTAFIDSSTGNSISYREMFRMV 98
           +G C S    ++ RE   +P + FLDV SFI S  H G T F+D+ TG  +S+ E++  V
Sbjct: 25  SGFCESTSIFYSKREPMALPPNQFLDVTSFIASQPHRGKTVFVDAVTGRRLSFPELWLGV 84

Query: 99  KSVASGLHKLGVSQGDVVLLVLPNSIFYPIILLGVLYLGAVITTMFPQSSSSEIKKRTSE 158
           + VA  L+ LGV +G+VV+++ PNSI +PI+ L V+ LGA+ITT  P ++S EI K+  +
Sbjct: 85  ERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEISKQIGD 144

Query: 159 CNVRLAFTIAQKIN--------NFETLGIQAIGVPENSNLDLIRPMG-FSSFHELISGGS 218
               LAFT  + ++        N   + +    VP  S  D ++ +G   +  E     S
Sbjct: 145 SRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIETEPSES 204

Query: 219 DLNKRPVIRQEDTAAILFSSGTTGVSKGVMLTQRNFISAIELF-VRFEASQYEYLPTENV 278
            + +R  + Q+DTAA+L+SSGTTG SKGVML+ RN I+ ++ +  RF   Q         
Sbjct: 205 RVKQR--VNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRARFGLEQRT------- 264

Query: 279 YLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDMVKAIDRFKVTHFPVVPPIMTSM 338
            +  IPM HI+G   F  GL++LG ++VV+ KFD+  ++ A++  + ++  +VPPI+ +M
Sbjct: 265 -ICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAM 324

Query: 339 ARTAEKIGV-HRFRSLKQVSSGAASLNKKTIDDFVQALPHVDFIQGYGMTESTALGTRGF 398
              A +I   +   SL  V +G A L+++  + FV+  P V  +QGYG+TESTA+    F
Sbjct: 325 VNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMF 384

Query: 399 NTKYARNYLSVGLLAPNMEAKVVD---------------WVTGSFMPPGYLNNPEATMST 458
           N +  + Y + GLLAPN+E K+VD               W+    +  GY  N EAT ST
Sbjct: 385 NKEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATAST 444

Query: 459 IDQENWLHTGDIVYFDREGYLYVVDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDAVVAA 518
           ID E WL TGD+ Y D +G+++VVDRLKE+IK  G+Q+AP +LEA+++ HPE+ DA V  
Sbjct: 445 IDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIP 504

Query: 519 AKDEECGEIPVAFVIKKPGSTLSQKDVIDYVAQQVAPYKKVRKVVFTESLPKSATGKVLR 558
             D + G+ P+A++++K GS LS+ +++ +VA+QV+PYKK+RKV F  S+PK+ +GK+LR
Sbjct: 505 IPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILR 564

BLAST of LsiUNG000790 vs. TAIR10
Match: AT1G20500.1 (AT1G20500.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 388.3 bits (996), Expect = 8.2e-108
Identity = 217/548 (39.60%), Postives = 336/548 (61.31%), Query Frame = 1

Query: 37  PDTGICRS---VHASRE---VPSDPFLDVVSFIFSFQHNGHTAFIDSSTGNSISYREMFR 96
           P +G C+S    ++ R+   +P +   DV +FI S  H G TAFID++TG  +++ +++R
Sbjct: 12  PRSGFCKSNSTFYSKRQPLSLPPNLSRDVTTFISSQPHRGKTAFIDAATGQCLTFSDLWR 71

Query: 97  MVKSVASGL-HKLGVSQGDVVLLVLPNSIFYPIILLGVLYLGAVITTMFPQSSSSEIKKR 156
            V  VA  L H++G+ +GDVVL++ PNSIF P++ L V+ LGAV TT    ++S EI K+
Sbjct: 72  AVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEISKQ 131

Query: 157 TSECNVRLAFTIAQKINNFETLGIQAIGVPENSNLDL---IRPMGFSSFHELISGG-SDL 216
            ++ N  L FT  Q       + I  +   +    +L   IR +G  S  E++    S  
Sbjct: 132 IADSNPTLVFTTRQLAPKLP-VAISVVLTDDEVYQELTSAIRVVGILS--EMVKKEPSGQ 191

Query: 217 NKRPVIRQEDTAAILFSSGTTGVSKGVMLTQRNFISAIELFVRFEASQYEYLPTENVYLA 276
             R  + Q+DTA +L+SSGTTG SKGV+ + RN  + +  F+       + L  +++++ 
Sbjct: 192 RVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFIS------DNLKRDDIFIC 251

Query: 277 AIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDMVKAIDRFKVTHFPVVPPIMTSMART 336
            +PMFH YGL  F MG ++LGS+VV++ +F + DM+ A+++ + T   + PP++ +M   
Sbjct: 252 TVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVLVAMIND 311

Query: 337 AEKIGV-HRFRSLKQVSSGAASLNKKTIDDFVQALPHVDFIQGYGMTESTALGTRGFNTK 396
           A+ I   +   SLK V  G A L+K+  + F++  P VD +QGY +TES   G    + +
Sbjct: 312 ADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGGAFTNSAE 371

Query: 397 YARNYLSVGLLAPNMEAKVVD---------------WVTGSFMPPGYLNNPEATMSTIDQ 456
            +R Y + G L  ++EA++VD               W+ G  +  GY  N EAT  TI+ 
Sbjct: 372 ESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEATNETINL 431

Query: 457 ENWLHTGDIVYFDREGYLYVVDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDAVVAAAKD 516
           E WL TGD+ Y D +G+L+VVDRLKE+IKYKG+Q+ P +LEA+++THP++LDA V    D
Sbjct: 432 EGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDAAVIPFPD 491

Query: 517 EECGEIPVAFVIKKPGSTLSQKDVIDYVAQQVAPYKKVRKVVFTESLPKSATGKVLRREL 558
           +E G+ P+A+V++K  S LS+K VID++++QVAPYKK+RKV F  S+PK+A+GK LR++L
Sbjct: 492 KEAGQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTASGKTLRKDL 550

BLAST of LsiUNG000790 vs. TAIR10
Match: AT4G05160.1 (AT4G05160.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 387.1 bits (993), Expect = 1.8e-107
Identity = 213/545 (39.08%), Postives = 340/545 (62.39%), Query Frame = 1

Query: 40  GICRSVHASREVPSDPFLDVVSFIF--SFQHNGHTAFIDSSTGNSISYREMFRMVKSVAS 99
           GI RS+  +  +P DP   +VSF+F  S  +    A  DS TG+S+++ ++   V  +A 
Sbjct: 10  GIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAH 69

Query: 100 GLHKLGVSQGDVVLLVLPNSIFYPIILLGVLYLGAVITTMFPQSSSSEIKKRTSECNVRL 159
           G H+LG+ + DVVL+  PNS  +P+  L V  +G V TT  P  + +E+ K+  + N ++
Sbjct: 70  GFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKI 129

Query: 160 AFTIAQ---KINNFET----LGIQ-AIGVPENSNLDLIRPMGFSSFHELISGGSDLNKRP 219
             ++ Q   KI  F+     LG +  + +P  SN  ++      SF  ++     +++ P
Sbjct: 130 IISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKIL------SFDNVMELSEPVSEYP 189

Query: 220 V--IRQEDTAAILFSSGTTGVSKGVMLTQRNFISAIELFVRFEASQYEYLPTENVYLAAI 279
              I+Q DTAA+L+SSGTTG SKGV LT  NFI+A  +    +    EY     V+L  +
Sbjct: 190 FVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEY---HGVFLCFL 249

Query: 280 PMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDMVKAIDRFKVTHFPVVPPIMTSMARTAE 339
           PMFH++GL++     +  G+++V M++F+++ ++K I++F+VTH  VVPP+  ++++ + 
Sbjct: 250 PMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSI 309

Query: 340 KIGVHRFRSLKQVSSGAASLNKKTIDDFVQALPHVDFIQGYGMTESTALGTRGFNTKYAR 399
            +      SLK + SGAA L K  +++  + +P+V  +QGYGMTE+  + +        R
Sbjct: 310 -VKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGKR 369

Query: 400 NYLSVGLLAPNMEAKVVDWVTGSFMPP---------------GYLNNPEATMSTIDQENW 459
           N  S G+LAP +EA++V   TG   PP               GYLNNP+AT  TID+++W
Sbjct: 370 NSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSW 429

Query: 460 LHTGDIVYFDREGYLYVVDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDAVVAAAKDEEC 519
           +HTGD+ YF+ +G LYVVDR+KE+IKYKGFQ+AP +LE ++++HP++LDAVV    DEE 
Sbjct: 430 VHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEA 489

Query: 520 GEIPVAFVIKKPGSTLSQKDVIDYVAQQVAPYKKVRKVVFTESLPKSATGKVLRRELGKH 558
           GE+P+AFV++ P S+++++D+  ++A+QVAPYK++R+V F   +PKSA GK+LRREL + 
Sbjct: 490 GEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQQ 544

BLAST of LsiUNG000790 vs. NCBI nr
Match: gi|449453328|ref|XP_004144410.1| (PREDICTED: 4-coumarate--CoA ligase-like 6 [Cucumis sativus])

HSP 1 Score: 952.6 bits (2461), Expect = 3.1e-274
Identity = 480/574 (83.62%), Postives = 523/574 (91.11%), Query Frame = 1

Query: 1   MARLLNDHFSFQTPENEAVITKKIPRSE--HKYPNWYSPDTGICRSVHASREVPSDPFLD 60
           MARL+N+ FSF+TPENEAVITKKIPRSE  +KYPNWYSPDTGICRSVH  R++PSDPFLD
Sbjct: 1   MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD 60

Query: 61  VVSFIFSFQ--HNGHTAFIDSSTGNSISYREMFRMVKSVASGLHKLGVSQGDVVLLVLPN 120
           VVSFIFSFQ  HNGH+A IDSSTGNSISY+E+F MVKS+ASGLH LG+SQGDVVLL+LPN
Sbjct: 61  VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN 120

Query: 121 SIFYPIILLGVLYLGAVITTMFPQSSSSEIKKRTSECNVRLAFTIAQKINNFETLGIQAI 180
           SIF+PII+LG LYLGAV+TTMFPQSSSSEIKKR ++CNVRLAF I QKI NFE LGI+ I
Sbjct: 121 SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI 180

Query: 181 GVPENSNLDLIRPMGFSSFHELISGGSDLNKRPVIRQEDTAAILFSSGTTGVSKGVMLTQ 240
           GVPEN+N +L+R MGFSSF+ELISGGSDL K+PVIRQEDTAAILFSSGTTGVSKGVML+ 
Sbjct: 181 GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH 240

Query: 241 RNFISAIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFD 300
           RNFIS IELFVRFEASQYEYLPT+NVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKFD
Sbjct: 241 RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD 300

Query: 301 VKDMVKAIDRFKVTHFPVVPPIMTSMARTAEKIGVHRFRSLKQVSSGAASLNKKTIDDFV 360
           VKD+VKAIDRFKVTHFPVVPPI+T++ARTAEKIGVHRFRSLKQVS GAA+ +KKTID+FV
Sbjct: 301 VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV 360

Query: 361 QALPHVDFIQGYGMTESTALGTRGFNTKYARNYLSVGLLAPNMEAKVVDWVTGSFMPP-- 420
            ALPHVDFIQGYGMTESTA+GTRGFNTK ARNYLSVGLLAPN EAKVVDWVTGS MPP  
Sbjct: 361 HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK 420

Query: 421 -------------GYLNNPEATMSTIDQENWLHTGDIVYFDREGYLYVVDRLKEVIKYKG 480
                        GYLNNPEAT  T+DQENWLHTGDIVYFDR+GYLYVVDRLKEVIKYKG
Sbjct: 421 TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG 480

Query: 481 FQIAPTDLEAVVMTHPEVLDAVVAAAKDEECGEIPVAFVIKKPGSTLSQKDVIDYVAQQV 540
           FQIAPTDLEAVV+THPEVLD  VAAAKDEECGEIPVAFV+KKPGS L+QKDV+DYVAQQV
Sbjct: 481 FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV 540

Query: 541 APYKKVRKVVFTESLPKSATGKVLRRELGKHLPS 556
           APYKKVRKV+FTES+PKSA GKVLRREL KH P+
Sbjct: 541 APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPA 574

BLAST of LsiUNG000790 vs. NCBI nr
Match: gi|659120993|ref|XP_008460452.1| (PREDICTED: 4-coumarate--CoA ligase-like 6 [Cucumis melo])

HSP 1 Score: 890.6 bits (2300), Expect = 1.4e-255
Identity = 456/552 (82.61%), Postives = 491/552 (88.95%), Query Frame = 1

Query: 1   MARLL-NDHFSFQTPENEAVITKKIPRSE--HKYPNWYSPDTGICRSVHASREVPSDPFL 60
           MARLL ND FSF+TPENEA ITKKIP SE  +KYPNWYSPDTGIC SVH  R++PSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFS--FQHNGHTAFIDSSTGNSISYREMFRMVKSVASGLHKLGVSQGDVVLLVLP 120
           DVVSFIFS  FQHNGH+A IDS TGNSISY+E+F MVKS+ASGLH LG+SQGDVVLL+LP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIILLGVLYLGAVITTMFPQSSSSEIKKRTSECNVRLAFTIAQKINNFETLGIQA 180
           NSIFYPII+LG LYLGAVITTMFPQSSSSEIKKR ++CNVRLAFTI QKI NFE LGI+ 
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENSNLDLIRPMGFSSFHELISGGSDLNKRPVIRQEDTAAILFSSGTTGVSKGVMLT 240
           IGVPEN+N DL+RPMGFSSF+ELIS GSDL KRPVIRQEDTAAILFSSGTTGVSKGVML+
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 QRNFISAIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
            RNFIS IELFVRFEASQYEYLPT+NVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDMVKAIDRFKVTHFPVVPPIMTSMARTAEKIGVHRFRSLKQVSSGAASLNKKTIDDF 360
           DVKD+VKAIDRFKVTHFPVVPPI+ +++RTAEKIGV RFRSLKQVS GAA+ +KKTIDDF
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VQALPHVDFIQGYGMTESTALGTRGFNTKYARNYLSVGLLAPNMEAKVVDWVTGSFMPP- 420
           VQAL HVDFIQGYGMTESTA+GTRGFN K  RNYLSVGLLAPN EAKVVDWVTGS MPP 
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 --------------GYLNNPEATMSTIDQENWLHTGDIVYFDREGYLYVVDRLKEVIKYK 480
                         GYLNNPEATMSTIDQENWLHTGDIVYFDR+GYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVMTHPEVLDAVVAAAKDEECGEIPVAFVIKKPGSTLSQKDVIDYVAQQ 533
           GFQIAPTDLEAVV+ HPEVLD  VAAAKDEECGEIPVAFV+KKPGS LSQKDV+DYVAQQ
Sbjct: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540

BLAST of LsiUNG000790 vs. NCBI nr
Match: gi|590680124|ref|XP_007040775.1| (Acyl:coa ligase [Theobroma cacao])

HSP 1 Score: 689.1 bits (1777), Expect = 6.3e-195
Identity = 350/572 (61.19%), Postives = 441/572 (77.10%), Query Frame = 1

Query: 1   MARLLNDHFSFQTPENEAVITKKIPRSEHKYPNWYSPDTGICRSVHASREVPSDPFLDVV 60
           MA  LN HFSFQ  E++            ++P+WYSP+TGI  S H+S  +P+D FLD+V
Sbjct: 1   MATSLNSHFSFQRSESKTT----------RFPDWYSPETGIYSSKHSSISLPTDHFLDIV 60

Query: 61  SFIFSFQHNGHTAFIDSSTGNSISYREMFRMVKSVASGLHKLGVSQGDVVLLVLPNSIFY 120
           SFIFS QH+G TA IDSS+G S SY ++  +++S+ASGLH LGVSQGDVVLL+LPNS+ +
Sbjct: 61  SFIFSHQHDGVTALIDSSSGYSTSYAKLLPLIQSMASGLHHLGVSQGDVVLLLLPNSVHF 120

Query: 121 PIILLGVLYLGAVITTMFPQSSSSEIKKRTSECNVRLAFTIAQKINNFETLGIQAIGVPE 180
           PII L VLYLGA++TTM P +S  EIKK+ ++ +VR AFT+  K++  + LG+ AIGVPE
Sbjct: 121 PIIFLSVLYLGAIVTTMNPLTSMLEIKKQIADSSVRFAFTLLDKVDKLKNLGVHAIGVPE 180

Query: 181 NSNLDLIRPMGFSSFHELISGGSDLNKRPVIRQEDTAAILFSSGTTGVSKGVMLTQRNFI 240
           N NLD  +   F  FH+LI G  D   RPVIRQ+DTAAI++SSGTTGVSKGV+LT  NFI
Sbjct: 181 NLNLDSEKT-DFLLFHKLIGGQFDKAPRPVIRQQDTAAIMYSSGTTGVSKGVVLTHGNFI 240

Query: 241 SAIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDM 300
           + +ELFVRFEASQYEY  ++NVYLA +PMFHIYGLS+FV+GL+SLG+S+VVM +F+  +M
Sbjct: 241 AMVELFVRFEASQYEYSSSKNVYLAVLPMFHIYGLSLFVVGLLSLGTSIVVMRRFNATEM 300

Query: 301 VKAIDRFKVTHFPVVPPIMTSMARTAEKIGVHRFRSLKQVSSGAASLNKKTIDDFVQALP 360
           V+ ID++ VTHFPVVPPI+ ++A  A+ +  +  +SLKQVS GAA L++K I+DFVQALP
Sbjct: 301 VRVIDKYGVTHFPVVPPILRTLAMRAKGVCENSLKSLKQVSCGAAPLSRKAIEDFVQALP 360

Query: 361 HVDFIQGYGMTESTALGTRGFNTKYARNYLSVGLLAPNMEAKVVDWVTGSFMPP------ 420
           HVDFIQGYGMTESTA+G RGFN +    Y SVGLLAPNM+AKVVDW +GS +PP      
Sbjct: 361 HVDFIQGYGMTESTAVGNRGFNNEKHHKYSSVGLLAPNMQAKVVDWNSGSSLPPDSHGEL 420

Query: 421 ---------GYLNNPEATMSTIDQENWLHTGDIVYFDREGYLYVVDRLKEVIKYKGFQIA 480
                    GYLNN EAT  TID++ WL TGDIV FD +GY+YV DRLKE+IKYKG+QIA
Sbjct: 421 WLRGPGLMQGYLNNVEATRMTIDKDGWLRTGDIVCFDEDGYMYVSDRLKEIIKYKGYQIA 480

Query: 481 PTDLEAVVMTHPEVLDAVVAAAKDEECGEIPVAFVIKKPGSTLSQKDVIDYVAQQVAPYK 540
           P DLEA++++HPE+LDA V  A DEE GEIPVAFV+++ GSTL+Q  V+D+VA+QVAPYK
Sbjct: 481 PADLEAILISHPEILDAAVIPAIDEEAGEIPVAFVVRRQGSTLTQGAVMDFVAKQVAPYK 540

Query: 541 KVRKVVFTESLPKSATGKVLRRELGKHLPSKL 558
           KVRKVVFT+S+PKSA GK LRREL   L S+L
Sbjct: 541 KVRKVVFTKSIPKSAAGKTLRRELRNFLSSRL 561

BLAST of LsiUNG000790 vs. NCBI nr
Match: gi|1009166294|ref|XP_015901516.1| (PREDICTED: 4-coumarate--CoA ligase-like 6 [Ziziphus jujuba])

HSP 1 Score: 684.5 bits (1765), Expect = 1.6e-193
Identity = 350/573 (61.08%), Postives = 445/573 (77.66%), Query Frame = 1

Query: 1   MARLLNDHFSFQTPENEAVITKKIPRSEHK-YPNWYSPDTGICRSVHASREVPSDPFLDV 60
           MA  LN+ F+    E      K+  +S H  +P+WYSP+TGI  S + S  +PSDPFLD 
Sbjct: 19  MATSLNNAFTSILSEE-----KESEKSLHAPFPHWYSPETGIYSSRNPSVHLPSDPFLDS 78

Query: 61  VSFIFSFQHNGHTAFIDSSTGNSISYREMFRMVKSVASGLHKLGVSQGDVVLLVLPNSIF 120
           VSFIFS +HNG +A IDSS+G+SISY E++R+V+S+ASGLH +G+SQGDVVL++LPN I+
Sbjct: 79  VSFIFSNRHNGVSALIDSSSGSSISYSELYRLVESMASGLHYMGISQGDVVLVLLPNCIY 138

Query: 121 YPIILLGVLYLGAVITTMFPQSSSSEIKKRTSECNVRLAFTIAQKINNFETLGIQAIGVP 180
           YPII LGVLYLG V+T M P S+ SEIKK+ ++CNV  AFT  +K+   + LG+ AIGVP
Sbjct: 139 YPIIFLGVLYLGGVVTAMNPSSTVSEIKKQIADCNVCFAFTDPEKVEKLQVLGVPAIGVP 198

Query: 181 ENSNLDLIRPMGFSSFHELISGGSDLNKRPVIRQEDTAAILFSSGTTGVSKGVMLTQRNF 240
           EN N D  +   FS+F++LIS    +  RP+I+Q DTAAI+ SSGTTGVSKGV+LT RNF
Sbjct: 199 ENVNFDS-KATCFSAFYKLISCQFSMVPRPMIKQLDTAAIMHSSGTTGVSKGVVLTHRNF 258

Query: 241 ISAIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKD 300
           I+ +ELFVRFEASQY+Y   +NVYLA +PMFHIYGLS+F+MGL+SLGS+VVVM KF++ +
Sbjct: 259 IAMVELFVRFEASQYDYSSLKNVYLAIVPMFHIYGLSLFIMGLLSLGSTVVVMKKFNINE 318

Query: 301 MVKAIDRFKVTHFPVVPPIMTSMARTAEKIGVHRFRSLKQVSSGAASLNKKTIDDFVQAL 360
            VKAIDR+KVTHFP+VPP++T++ R+A+ +    FRSLKQVS GAA L+ + I++FVQ  
Sbjct: 319 AVKAIDRYKVTHFPLVPPMLTALTRSAKDVDWKSFRSLKQVSCGAAPLSWRIIEEFVQTF 378

Query: 361 PHVDFIQGYGMTESTALGTRGFNTKYARNYLSVGLLAPNMEAKVVDWVTGSFMPP----- 420
           PHVDFIQGYG+TESTA+GTRGFNT+  RNY S+GLLAPNM+AKVVD   GSF+PP     
Sbjct: 379 PHVDFIQGYGLTESTAVGTRGFNTEKFRNYSSIGLLAPNMQAKVVDSNIGSFLPPGKSGE 438

Query: 421 ----------GYLNNPEATMSTIDQENWLHTGDIVYFDREGYLYVVDRLKEVIKYKGFQI 480
                     GYLNN EATM ++D++ W+HTGDIVYFD +GYLY+ DRLKEVIKYKGFQI
Sbjct: 439 LWLRGPAVMQGYLNNAEATMLSVDKDGWMHTGDIVYFDGDGYLYLFDRLKEVIKYKGFQI 498

Query: 481 APTDLEAVVMTHPEVLDAVVAAAKDEECGEIPVAFVIKKPGSTLSQKDVIDYVAQQVAPY 540
           AP DLEAV++ HPE+ D  +A   D+E GEIPVAFV+KK  S+LS+ DV+DYVA +VAP+
Sbjct: 499 APADLEAVLICHPEIEDVAIAGTMDQESGEIPVAFVVKKHRSSLSEADVMDYVAAKVAPH 558

Query: 541 KKVRKVVFTESLPKSATGKVLRRELGKHLPSKL 558
           KKVRKVVFT S+PKSA GK+LR++L   L S+L
Sbjct: 559 KKVRKVVFTASIPKSAAGKILRKDLKNLLSSRL 585

BLAST of LsiUNG000790 vs. NCBI nr
Match: gi|703152441|ref|XP_010110411.1| (4-coumarate--CoA ligase-like 6 [Morus notabilis])

HSP 1 Score: 683.7 bits (1763), Expect = 2.7e-193
Identity = 341/544 (62.68%), Postives = 430/544 (79.04%), Query Frame = 1

Query: 30  KYPNWYSPDTGICRSVHASREVPSDPFLDVVSFIFSFQHNGHTAFIDSSTGNS-ISYREM 89
           ++P+WYSP+TGIC S +     PSDPFLDVVSFIFS QHNG +A +DSS+G+S ISY E+
Sbjct: 12  QFPHWYSPETGICHSTNRPITFPSDPFLDVVSFIFSKQHNGVSALVDSSSGSSSISYSEL 71

Query: 90  FRMVKSVASGLHKLGVSQGDVVLLVLPNSIFYPIILLGVLYLGAVITTMFPQSSSSEIKK 149
            R+VKS+ASGLH +GVS+ DVVLL+LPNS+++P+ILLGV+YLGA++TTM P S+ +E+KK
Sbjct: 72  HRLVKSMASGLHSMGVSKNDVVLLLLPNSVYFPVILLGVVYLGAIVTTMNPLSTVTEVKK 131

Query: 150 RTSECNVRLAFTIAQKINNFETLGIQAIGVPENSNLDLIRPMGFSSFHELISGGSDLNKR 209
           + ++    LAFT+ +K+       + AI VPEN N        F  F++L+S   D   R
Sbjct: 132 QVADSKACLAFTLPEKVELLRAFRVGAIAVPENFNSSQKPMSSFREFYKLVSCEFDFAPR 191

Query: 210 PVIRQEDTAAILFSSGTTGVSKGVMLTQRNFISAIELFVRFEASQYEYLPTENVYLAAIP 269
           P+IRQ+DTAAI++SSGTTG SKGV+LT  NFI+ ++LFVRFEASQYEY  +ENVYLA +P
Sbjct: 192 PLIRQQDTAAIMYSSGTTGASKGVVLTHGNFIAMVDLFVRFEASQYEYPRSENVYLAVLP 251

Query: 270 MFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDMVKAIDRFKVTHFPVVPPIMTSMARTAEK 329
           MFHIYGL++FVMGL+SLGS++VVM KFDV DMVK I+ +KVTHFPVVPP++ ++ + A+ 
Sbjct: 252 MFHIYGLALFVMGLLSLGSTIVVMRKFDVNDMVKTIETYKVTHFPVVPPMLIALTKCAKV 311

Query: 330 IGVHRFRSLKQVSSGAASLNKKTIDDFVQALPHVDFIQGYGMTESTALGTRGFNTKYARN 389
           +G   FRSLKQVSSGAA ++ K I+DF+  LPHVDFIQGYGMTESTA+GTRGFNTK  R 
Sbjct: 312 VGGKGFRSLKQVSSGAAPISGKIIEDFLHTLPHVDFIQGYGMTESTAVGTRGFNTKKCRK 371

Query: 390 YLSVGLLAPNMEAKVVDWVTGSFMPP---------------GYLNNPEATMSTIDQENWL 449
           + SVGLL PNMEAKV+D  TGSF+PP               GYLNN  ATMS ID++ WL
Sbjct: 372 FSSVGLLGPNMEAKVMDLNTGSFLPPGSCGELWLRGPAVMKGYLNNAVATMSAIDKDGWL 431

Query: 450 HTGDIVYFDREGYLYVVDRLKEVIKYKGFQIAPTDLEAVVMTHPEVLDAVVAAAKDEECG 509
            TGDIVYFD++GYL+VVDRLKE+IKYKG+QIAP DLEAV+ +HPE+ D  VAAA+D + G
Sbjct: 432 RTGDIVYFDQDGYLHVVDRLKEIIKYKGYQIAPADLEAVLASHPEIRDVAVAAAEDADSG 491

Query: 510 EIPVAFVIKKPGSTLSQKDVIDYVAQQVAPYKKVRKVVFTESLPKSATGKVLRRELGKHL 558
           E+PVAFV++K GS+LSQ+ V+DYVA+QVAPYKK+RKVVFT S+PKSA GK+LRREL K L
Sbjct: 492 EVPVAFVVRKHGSSLSQEAVMDYVARQVAPYKKIRKVVFTHSIPKSAAGKILRRELQKSL 551