MELO3C017001 (gene) Melon (DHL92) v3.5.1

BLAST of MELO3C017001 vs. Swiss-Prot
Match: PUS1_CANAL (tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=PUS1 PE=3 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 5.4e-39
Identity = 120/378 (31.75%), Postives = 186/378 (49.21%), Query Frame = 1

Query: 103 KRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAK 162
           K+  + R+ARTDKGV A G V+S +  I+ P  ++++N  L  QIRI+G +R T  F+ +
Sbjct: 37  KKSGFQRAARTDKGVHAAGNVISLKMIIEDPEIINKINDLLPKQIRIWGIQRATKGFDCR 96

Query: 163 KFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNF 222
           K C  R Y YL+P F+L P      +SV + L  + EL +  +  E  +  EG+    N 
Sbjct: 97  KCCSSRIYEYLLPTFSLLP---PKPKSVLSELVKEKEL-ENPDLFEDDQ--EGIDWWEN- 156

Query: 223 EVKTAITESDISSNIVNAIEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEG 282
            VK+ I  S I+   +++I             +++  +  +Q     +  A E       
Sbjct: 157 -VKSKILASGITQEQIDSI-------------TSSYDEQQQQQHHQQQQQADE------- 216

Query: 283 IAKSESSEMSGLGNDSEI-VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFH 342
             + + SE+S      +I  +  +S       S   ++  E         K Y GTHNFH
Sbjct: 217 -EERDISELSFTKLIKQIKTIENQSRRSYRISSSRLQHFREVM-------KQYEGTHNFH 276

Query: 343 NFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMR 402
           NFT     +D +A RF++    S   V+EG E++  ++ GQSFMLHQIRKM+ +A  ++R
Sbjct: 277 NFTVGKPFKDTSANRFMIKTIVSDPFVIEGTEWISIKIHGQSFMLHQIRKMIAMAALVVR 336

Query: 403 NCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQK-WKDTHEEVSMKAYEEVAE 462
              P  +I    Q    IN+P AP +GL L+  +F  YN K  K  +E +    +++   
Sbjct: 337 LSLPCGIINNFFQ-STKINIPKAPALGLLLENPVFDGYNIKLTKSDYEPIDFTKFDKEMN 377

Query: 463 DFKMKQIYSHIASTEHKD 479
           +FKMK IY  I + E K+
Sbjct: 397 EFKMKYIYDKIYAEESKE 377

BLAST of MELO3C017001 vs. Swiss-Prot
Match: TRUA_MOUSE (tRNA pseudouridine synthase A, mitochondrial OS=Mus musculus GN=Pus1 PE=1 SV=2)

HSP 1 Score: 138.3 bits (347), Expect = 2.4e-31
Identity = 74/180 (41.11%), Postives = 108/180 (60.00%), Query Frame = 1

Query: 316 SFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFV 375
           S++   E   + NR+L  Y GTHNFHNFT++    +P+ARR+I+        V EG+EF 
Sbjct: 217 SYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFA 276

Query: 376 KCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECL 435
             +V GQSFM+HQIRKM+GL VAI++  APES++E++  ++  ++VP AP +GL L+   
Sbjct: 277 VIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEE-KVDVPKAPGLGLVLERVH 336

Query: 436 FTSYNQKW--KDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNY 494
           F  YNQ++     HE +     E     FK + IY  I STE  + ++A WL++L   N+
Sbjct: 337 FEKYNQRFGGDGLHEPLDWTQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNF 395

HSP 2 Score: 122.5 bits (306), Expect = 1.4e-26
Identity = 78/210 (37.14%), Postives = 108/210 (51.43%), Query Frame = 1

Query: 5   PPTETRRSPPQPLIEEPDPK-KLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAY 64
           PP    + PP     +PD K +       T    + V         ++  +RK+ +  AY
Sbjct: 29  PPMAGNKVPPALASHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPRKLPKRKIVLLMAY 88

Query: 65  CGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 124
            G GY GMQ+N G+   +TIE DL  AL  AG +PE      ++  + R ARTDKGVSA 
Sbjct: 89  SGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAA 148

Query: 125 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 184
           GQVVS + ++     +D++NS+L   IRI G KRVT  FN+K  CD R Y Y++P FA  
Sbjct: 149 GQVVSLKVWLIDD-ILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAF- 208

Query: 185 PNCHRDR----ESVKASLGSDNELVKCLEC 207
              H+DR    ES + S  +  ++ + L C
Sbjct: 209 --AHKDRDVQDESYRLSAETLQQVNRLLAC 234

BLAST of MELO3C017001 vs. Swiss-Prot
Match: TRUA_RAT (tRNA pseudouridine synthase A, mitochondrial OS=Rattus norvegicus GN=Pus1 PE=1 SV=2)

HSP 1 Score: 137.9 bits (346), Expect = 3.2e-31
Identity = 74/180 (41.11%), Postives = 108/180 (60.00%), Query Frame = 1

Query: 316 SFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFV 375
           S++   E   + NR+L  Y GTHNFHNFT++    +P+ARR+I+        V EG+EF 
Sbjct: 217 SYRLSAETLQQVNRLLGCYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFA 276

Query: 376 KCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECL 435
             +V GQSFM+HQIRKM+GL VAI++  APES++E++  ++  ++VP AP +GL L+   
Sbjct: 277 VIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEE-KVDVPKAPGLGLVLERVH 336

Query: 436 FTSYNQKW--KDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNY 494
           F  YNQ++     HE +     E     FK + IY  I STE  + ++A WL++L   N+
Sbjct: 337 FEKYNQRFGSDGLHEPLDWAQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNF 395

HSP 2 Score: 121.3 bits (303), Expect = 3.1e-26
Identity = 78/210 (37.14%), Postives = 108/210 (51.43%), Query Frame = 1

Query: 5   PPTETRRSPPQPLIEEPDPK-KLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAY 64
           PP    + PP     +PD K +       T    + V         ++  +RK+ +  AY
Sbjct: 29  PPMAGNKVPPALPSHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPRKLPKRKIVLLMAY 88

Query: 65  CGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 124
            G GY GMQ+N G+   +TIE DL  AL  AG +PE      ++  + R ARTDKGVSA 
Sbjct: 89  SGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAA 148

Query: 125 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 184
           GQVVS + ++     +D++NS+L   IRI G KRVT  FN+K  CD R Y Y++P FA  
Sbjct: 149 GQVVSLKVWLIDD-ILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAF- 208

Query: 185 PNCHRDR----ESVKASLGSDNELVKCLEC 207
              H+DR    ES + S  +  ++ + L C
Sbjct: 209 --AHKDRDVQDESYRLSAETLQQVNRLLGC 234

BLAST of MELO3C017001 vs. Swiss-Prot
Match: TRUA_HUMAN (tRNA pseudouridine synthase A, mitochondrial OS=Homo sapiens GN=PUS1 PE=1 SV=3)

HSP 1 Score: 133.3 bits (334), Expect = 7.8e-30
Identity = 72/180 (40.00%), Postives = 105/180 (58.33%), Query Frame = 1

Query: 316 SFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFV 375
           +++   E   + NR+L  Y GTHNFHNFT++   +DP+A R+I+        V EG+EF 
Sbjct: 221 TYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFA 280

Query: 376 KCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECL 435
              V GQSFM+HQIRKM+GL VAI++  APES++E++   +  ++VP AP +GL L+   
Sbjct: 281 VIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTE-KVDVPKAPGLGLVLERVH 340

Query: 436 FTSYNQKWKD--THEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNY 494
           F  YNQ++ +   HE +     E     FK + IY  I  TE  + ++A WL +L   N+
Sbjct: 341 FEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNF 399

HSP 2 Score: 119.4 bits (298), Expect = 1.2e-25
Identity = 65/152 (42.76%), Postives = 90/152 (59.21%), Query Frame = 1

Query: 40  VNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPE 99
           + S     ++++  +RK+ +  AY G GY GMQ+N G+   KTIE DL  AL  +G +PE
Sbjct: 69  LKSGGDEERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPE 128

Query: 100 QDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVT 159
                 ++  + R ARTDKGVSA GQVVS + ++     ++++NS+L   IRI G KRVT
Sbjct: 129 NHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDD-ILEKINSHLPSHIRILGLKRVT 188

Query: 160 ASFNAKKFCDRRRYVYLIPVFALDPNCHRDRE 189
             FN+K  CD R Y YL+P FA     H+DR+
Sbjct: 189 GGFNSKNRCDARTYCYLLPTFAF---AHKDRD 216

BLAST of MELO3C017001 vs. Swiss-Prot
Match: PUS1_ASHGO (tRNA pseudouridine synthase 1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) GN=PUS1 PE=3 SV=2)

HSP 1 Score: 122.5 bits (306), Expect = 1.4e-26
Identity = 61/152 (40.13%), Postives = 88/152 (57.89%), Query Frame = 1

Query: 27  KMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEADLEE 86
           K +    T D +   + ++  K+ R  +RKVA+   YCG GY GMQ NP  +TIEA+L E
Sbjct: 39  KEAKRPCTEDSDVALAGTSEEKEARLPKRKVAVMVGYCGTGYHGMQYNPPNRTIEAELFE 98

Query: 87  ALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQ 146
           A   AGA+   +    K+  + R+ARTDKGV A G V+S +  I+ P   D++N +L P 
Sbjct: 99  AFVKAGAISRANSTDLKKNGFMRAARTDKGVHAGGNVISLKLIIEDPAIKDKINEHLPPG 158

Query: 147 IRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 179
           IR++G  RV  +F+ +K C  R Y YL+P ++
Sbjct: 159 IRVWGISRVNKAFDCRKLCGSRWYEYLLPTYS 190

HSP 2 Score: 107.1 bits (266), Expect = 6.0e-22
Identity = 64/196 (32.65%), Postives = 102/196 (52.04%), Query Frame = 1

Query: 284 AKSESSEMSGLGNDSEIVVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNF 343
           +K +  E SGL    +     E+ + +     S++    + ARF   +K Y+G HNFHN+
Sbjct: 251 SKEDFDENSGLYQKVKQYKQMENAHRR-----SYRVSSAKLARFREAMKQYLGPHNFHNY 310

Query: 344 TTRTKAEDPAARRFIVSFDASTTVVVEGI--EFVKCEVVGQSFMLHQIRKMMGLAVAIMR 403
           T     +DP+  RF+     S   V+  +  E+V  ++ GQSFMLHQIRKM+ +A  + R
Sbjct: 311 TLGKDFKDPSTVRFMKDITVSDPFVIGEMKTEWVSIKIHGQSFMLHQIRKMISMATLVAR 370

Query: 404 -NCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKD-THEEVSMKAYEEVA 463
            NC+PE + +        IN+P AP +GL L+  ++  YN++  +  +E +  + Y++  
Sbjct: 371 CNCSPERIAQS--YGPQKINIPKAPALGLLLESPVYEGYNKRLLEFGYEPIDFRNYQKEM 430

Query: 464 EDFKMKQIYSHIASTE 476
           + FKM  IY  I   E
Sbjct: 431 DTFKMVHIYDKIYKEE 439

BLAST of MELO3C017001 vs. TrEMBL
Match: E5GCD2_CUCME (tRNA pseudouridine synthase family protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 1031.9 bits (2667), Expect = 2.6e-298
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 1

Query: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60
           MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240

Query: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300
           IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300

Query: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 520
           VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 519

BLAST of MELO3C017001 vs. TrEMBL
Match: A0A0A0L0J9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G652870 PE=4 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 8.9e-291
Identity = 506/519 (97.50%), Postives = 509/519 (98.07%), Query Frame = 1

Query: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60
           MEEEPPTETRRSPPQPLIEEP PKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGL+GKRNFEVKTAITESDISSN VNA
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNA 240

Query: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300
           IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATEL V SEG AKSESSEMSGLG+DSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEI 300

Query: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNGKEK SGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDAS  VVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEE AEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 520
           VALWLHSLNHRNYPDLRVIDEVAKIAKN S+DMETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQAET 519

BLAST of MELO3C017001 vs. TrEMBL
Match: A0A0S3R6B4_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G417100 PE=4 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 6.4e-196
Identity = 358/524 (68.32%), Postives = 417/524 (79.58%), Query Frame = 1

Query: 3   EEPPTETRRS---PPQPLI-EEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVA 62
           E P T    S   PP PL  +EP+PKKLKMS  TTTSDDE+  +  A   K+RYKRRKVA
Sbjct: 2   ENPDTALTTSSLPPPPPLAADEPEPKKLKMS--TTTSDDEECKT--APGTKKRYKRRKVA 61

Query: 63  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 122
           IFFAYCGVGYQGMQKNPGAKTIE DLEEALY++GAVPE DRG+ KRYDWARSARTDKGVS
Sbjct: 62  IFFAYCGVGYQGMQKNPGAKTIEGDLEEALYVSGAVPEHDRGIPKRYDWARSARTDKGVS 121

Query: 123 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 182
           AVGQVVSGRFYIDPPG VDRLNSNL  QIRIFGYKRVT SF+AKKFCDRRRYVYLIPVFA
Sbjct: 122 AVGQVVSGRFYIDPPGLVDRLNSNLPSQIRIFGYKRVTGSFSAKKFCDRRRYVYLIPVFA 181

Query: 183 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSN-- 242
           LDP+CHRDRE+V ASLGS NELVKCLECSERGRKV GL+G R   ++    + D  SN  
Sbjct: 182 LDPSCHRDRETVMASLGSTNELVKCLECSERGRKVVGLVGNRKHNLELEAMDVDAGSNRD 241

Query: 243 -IVNAIEVSDVQAN-GVLEDSATTSDSNKQTS--VLVENTATELTVASEGIAKSESSEMS 302
            +++++   DV+ +    +D+    +S   T   VLV+ T  E  + +    + E   ++
Sbjct: 242 VVLDSVVAEDVEVSLSKGDDNHLNGESGIDTKGEVLVDGTNPETGIENMITVQDEDMPLN 301

Query: 303 GLGNDSEIVVSKESVNGKEKPSGS--FKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAE 362
           G   ++  ++ +E +NG +K +G   F YGE+E+ RFN++L  +VGTHNFHNFTTRTKA+
Sbjct: 302 GESVNNSDIIEEEKMNGVDKSTGGSRFCYGEKERERFNKVLNFFVGTHNFHNFTTRTKAQ 361

Query: 363 DPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIE 422
           DPAARR+I+SF+A+TTVVVEGIEFVKCEVVGQSFMLHQIRKM+GLAVAIMRNCAPESLI 
Sbjct: 362 DPAARRYIISFNANTTVVVEGIEFVKCEVVGQSFMLHQIRKMIGLAVAIMRNCAPESLIN 421

Query: 423 KALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSH 482
           KALQ+DVNINVPTAPEVGLYLDEC F+SYNQKWKD+HEEVSMKAYE+ AE+FKMK IYSH
Sbjct: 422 KALQQDVNINVPTAPEVGLYLDECFFSSYNQKWKDSHEEVSMKAYEKEAEEFKMKYIYSH 481

Query: 483 IASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDME 515
           I+STE K+G VALWLHSLNHRNYPDLRV++E A    N S D E
Sbjct: 482 ISSTEQKEGTVALWLHSLNHRNYPDLRVVNEEA-TTDNKSADPE 520

BLAST of MELO3C017001 vs. TrEMBL
Match: A0A0L9TQX3_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g245700 PE=4 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 6.4e-196
Identity = 358/524 (68.32%), Postives = 417/524 (79.58%), Query Frame = 1

Query: 3   EEPPTETRRS---PPQPLI-EEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVA 62
           E P T    S   PP PL  +EP+PKKLKMS  TTTSDDE+  +  A   K+RYKRRKVA
Sbjct: 2   ENPDTALTTSSLPPPPPLAADEPEPKKLKMS--TTTSDDEECKT--APGTKKRYKRRKVA 61

Query: 63  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 122
           IFFAYCGVGYQGMQKNPGAKTIE DLEEALY++GAVPE DRG+ KRYDWARSARTDKGVS
Sbjct: 62  IFFAYCGVGYQGMQKNPGAKTIEGDLEEALYVSGAVPEHDRGIPKRYDWARSARTDKGVS 121

Query: 123 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 182
           AVGQVVSGRFYIDPPG VDRLNSNL  QIRIFGYKRVT SF+AKKFCDRRRYVYLIPVFA
Sbjct: 122 AVGQVVSGRFYIDPPGLVDRLNSNLPSQIRIFGYKRVTGSFSAKKFCDRRRYVYLIPVFA 181

Query: 183 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSN-- 242
           LDP+CHRDRE+V ASLGS NELVKCLECSERGRKV GL+G R   ++    + D  SN  
Sbjct: 182 LDPSCHRDRETVMASLGSTNELVKCLECSERGRKVVGLVGNRKHNLELEAMDVDAGSNRD 241

Query: 243 -IVNAIEVSDVQAN-GVLEDSATTSDSNKQTS--VLVENTATELTVASEGIAKSESSEMS 302
            +++++   DV+ +    +D+    +S   T   VLV+ T  E  + +    + E   ++
Sbjct: 242 VVLDSVVAEDVEVSLSKGDDNHLNGESGIDTKGEVLVDGTNPETGIENMITVQDEDMPLN 301

Query: 303 GLGNDSEIVVSKESVNGKEKPSGS--FKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAE 362
           G   ++  ++ +E +NG +K +G   F YGE+E+ RFN++L  +VGTHNFHNFTTRTKA+
Sbjct: 302 GESVNNSDIIEEEKMNGVDKSTGGSRFCYGEKERERFNKVLNFFVGTHNFHNFTTRTKAQ 361

Query: 363 DPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIE 422
           DPAARR+I+SF+A+TTVVVEGIEFVKCEVVGQSFMLHQIRKM+GLAVAIMRNCAPESLI 
Sbjct: 362 DPAARRYIISFNANTTVVVEGIEFVKCEVVGQSFMLHQIRKMIGLAVAIMRNCAPESLIN 421

Query: 423 KALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSH 482
           KALQ+DVNINVPTAPEVGLYLDEC F+SYNQKWKD+HEEVSMKAYE+ AE+FKMK IYSH
Sbjct: 422 KALQQDVNINVPTAPEVGLYLDECFFSSYNQKWKDSHEEVSMKAYEKEAEEFKMKYIYSH 481

Query: 483 IASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDME 515
           I+STE K+G VALWLHSLNHRNYPDLRV++E A    N S D E
Sbjct: 482 ISSTEQKEGTVALWLHSLNHRNYPDLRVVNEEA-TTDNKSADPE 520

BLAST of MELO3C017001 vs. TrEMBL
Match: I1JGM9_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_02G202300 PE=4 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 2.4e-195
Identity = 367/525 (69.90%), Postives = 421/525 (80.19%), Query Frame = 1

Query: 1   MEEEPPTETRRS-PPQPLI--EEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKV 60
           ME    T T  S PP P +  EEP+PKKLKMS  TTTSDDE+  +      K+RYKRRKV
Sbjct: 1   MENSDTTLTSSSLPPPPPVSAEEPEPKKLKMS--TTTSDDEETGT------KKRYKRRKV 60

Query: 61  AIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGV 120
           AIFFAYCGVGYQGMQKNPGAKTIE DLEEALY++GAVPEQDRG+ KRYDWARSARTDKGV
Sbjct: 61  AIFFAYCGVGYQGMQKNPGAKTIEGDLEEALYVSGAVPEQDRGVPKRYDWARSARTDKGV 120

Query: 121 SAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVF 180
           SAVGQVVSGRFYIDPPG VDRLNSNL  QIRIFGYKRVTASFNAKKFCDRRRYVYLIPVF
Sbjct: 121 SAVGQVVSGRFYIDPPGLVDRLNSNLPSQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVF 180

Query: 181 ALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMG--KRNFEVKTAITESDISS 240
           ALDP+CHRDRE+V ASLGS NELVKCLECSERGRKV GL+G  K+N E++    E+ ISS
Sbjct: 181 ALDPSCHRDRETVMASLGSGNELVKCLECSERGRKVVGLVGNRKQNLELEAVDVEAGISS 240

Query: 241 N----IVNAIEVSDVQA--NGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESS 300
           N    +   +EVS  +   N + E+S   +         +E   T + V  EG      +
Sbjct: 241 NRDSGVTEDVEVSLSKGDDNHLNEESGNDNKGGMNCKTGLE---TVIPVQEEG------T 300

Query: 301 EMSGLGNDSEIVVSKESVNGKEKPSGS--FKYGEEEKARFNRILKHYVGTHNFHNFTTRT 360
            ++G   ++  ++ +E VNG++KP+    F YGE+E+ RFN+ILK +VGTHNFHNFTTRT
Sbjct: 301 PLNGESVNNSDILEEEKVNGEDKPTNGSRFCYGEKERERFNKILKCFVGTHNFHNFTTRT 360

Query: 361 KAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPES 420
           KAEDPAARR+I+SF+A+TT+VVEG+EFVKCEVVGQSFMLHQIRKM+GLAVAIMRNCAPES
Sbjct: 361 KAEDPAARRYIISFNANTTLVVEGVEFVKCEVVGQSFMLHQIRKMIGLAVAIMRNCAPES 420

Query: 421 LIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQI 480
           LI++ALQ+DVNINVPTAPEVGLYLDEC FTSYNQKWKD HEE+SMKAYE+ A++FKMK I
Sbjct: 421 LIDEALQQDVNINVPTAPEVGLYLDECFFTSYNQKWKD-HEELSMKAYEKEADEFKMKYI 480

Query: 481 YSHIASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAKNTSVD 513
           YSHIASTE K+G VALWLHSLNHRNYPDLRVI+E A I  N S D
Sbjct: 481 YSHIASTEQKEGTVALWLHSLNHRNYPDLRVINEEA-IPNNKSAD 506

BLAST of MELO3C017001 vs. TAIR10
Match: AT1G20370.1 (AT1G20370.1 Pseudouridine synthase family protein)

HSP 1 Score: 564.3 bits (1453), Expect = 7.8e-161
Identity = 300/504 (59.52%), Postives = 367/504 (72.82%), Query Frame = 1

Query: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVN---SNSAAPKKQRYKRRKV 60
           ME + P   +         EPD KKLK+S+T   +D   V    SN A  +  +Y+RRKV
Sbjct: 1   MENQEPDIEKLPRDDVPDREPDHKKLKISTTGQETDSSAVTTGGSNVAERRAPKYRRRKV 60

Query: 61  AIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGV 120
           AI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ AGAVP+ DR   + Y+WARSARTDKGV
Sbjct: 61  AIVFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPDADRNKPRNYEWARSARTDKGV 120

Query: 121 SAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVF 180
           SAVGQVVSGRFY+DPPGFV+RLNS L  QIR+FGYKRV  SF++KKFCDRRRYVYLIPVF
Sbjct: 121 SAVGQVVSGRFYVDPPGFVERLNSKLPDQIRVFGYKRVAPSFSSKKFCDRRRYVYLIPVF 180

Query: 181 ALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVE-GLMGKRN--FEVKTAITESDIS 240
           ALDP  H + E V+  LG   E VKC+ECSE+G K+  G+MGK     + K+   +SDIS
Sbjct: 181 ALDPCVHGEAEMVRTDLGY--EYVKCVECSEKGYKIPLGVMGKDTDCCDTKSLEIQSDIS 240

Query: 241 SNIVNAIEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGL 300
           S+  +AI  +DV+    L  S  ++++N  + VL     + L+  +EG+ +S +     +
Sbjct: 241 SSTCDAIG-ADVKCE-TLSSSVPSAENNLNSEVLDGADVSALSSKAEGMEESNTLAKVEM 300

Query: 301 GN-DSEIVVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPA 360
            N + E +   ES          F YGE+EK RFNRIL +YVG+HNFHNFTTRTKA DPA
Sbjct: 301 NNGEGEDMTKSES---------KFCYGEKEKERFNRILSYYVGSHNFHNFTTRTKAADPA 360

Query: 361 ARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKAL 420
           A R+I+SF+A+T + ++G +FVKCEVVGQSFMLHQIRKM+GLAVAIMRN APESLIE + 
Sbjct: 361 ANRYILSFNANTVINLDGKDFVKCEVVGQSFMLHQIRKMIGLAVAIMRNYAPESLIEASF 420

Query: 421 QKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIAS 480
           +KDV INVP APEVGLYLDEC FTSYN+++K +HEEVSM+ Y+EVAE+FK K +YSHI S
Sbjct: 421 KKDVRINVPMAPEVGLYLDECFFTSYNKRFKGSHEEVSMEEYKEVAEEFKWKYVYSHIGS 480

Query: 481 TEHKDGAVALWLHSLNHRNYPDLR 498
            E KDGAVA+WLHSLN RNYPDLR
Sbjct: 481 AEEKDGAVAIWLHSLNQRNYPDLR 491

BLAST of MELO3C017001 vs. TAIR10
Match: AT1G76120.1 (AT1G76120.1 Pseudouridine synthase family protein)

HSP 1 Score: 533.1 bits (1372), Expect = 1.9e-151
Identity = 284/470 (60.43%), Postives = 340/470 (72.34%), Query Frame = 1

Query: 31  TTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYL 90
           TTTT ++ D++ +    + Q     KVAI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ 
Sbjct: 3   TTTTDEEIDLSCDGGVTEPQ-----KVAIIFAFCGVGYQGMQKNPGAKTIEGELEEALFH 62

Query: 91  AGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIF 150
           AGAVPE  RG  K YD+ARSARTDKGVSAVGQVVSGRF +DP GFV+RLNSNL  QIRIF
Sbjct: 63  AGAVPESIRGKPKLYDFARSARTDKGVSAVGQVVSGRFIVDPLGFVNRLNSNLPNQIRIF 122

Query: 151 GYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECSERG 210
           GYK VT SF++KKFCDRRRYVYL+PVFALDP  HRDRE+V ASLG   E VKC ECSERG
Sbjct: 123 GYKHVTPSFSSKKFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERG 182

Query: 211 RKV-EGLMGK---RNFEVKTAITESDISSNIVNAIEVSDVQANGVLEDSATTSDSNKQTS 270
           RK+  GL+GK    NF  K+   +SDISSN  +A+  SD++   +  + A     + +  
Sbjct: 183 RKIPPGLVGKWKGTNFGTKSLDFQSDISSNNSSALR-SDIKIEALSSNLAGLCSVDVEVG 242

Query: 271 VLVENTATELTVASEGIAKSESSEMSGLGNDSEIVVSKESVNGKEKPSGSFKYGEEEKAR 330
            + E++    T +SE   KS+                             F YGE+EK R
Sbjct: 243 RIQEDSCKLNTNSSETKVKSK-----------------------------FCYGEKEKER 302

Query: 331 FNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFML 390
           F+RIL  YVG++NFHNFTTRTKA+DP A R I+SF A+T + ++GI+F+KCEV+G+SFML
Sbjct: 303 FSRILSCYVGSYNFHNFTTRTKADDPTANRQIISFTANTVINLDGIDFIKCEVLGKSFML 362

Query: 391 HQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDT 450
           HQIRKMMGLAVAIMRNCA ESLI+ A  KDVNI VP APEVGLYLDEC FTSYN+ ++D+
Sbjct: 363 HQIRKMMGLAVAIMRNCASESLIQSAFSKDVNITVPMAPEVGLYLDECFFTSYNRNFEDS 422

Query: 451 HEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDL 497
           HEEVSM+AY+E AE FK+K IYSHI +TE K G +ALWLHSLN+RNYPDL
Sbjct: 423 HEEVSMEAYKEEAEAFKLKHIYSHIGATERKYGNMALWLHSLNYRNYPDL 437

BLAST of MELO3C017001 vs. TAIR10
Match: AT2G30320.1 (AT2G30320.1 Pseudouridine synthase family protein)

HSP 1 Score: 94.4 bits (233), Expect = 2.3e-19
Identity = 77/274 (28.10%), Postives = 125/274 (45.62%), Query Frame = 1

Query: 23  PKKLKMSST-----TTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQ---KN 82
           P  L+ SS+     ++TS     + N  A K + Y+++KV I   Y G  Y+G+Q    +
Sbjct: 26  PALLRFSSSAAFQPSSTSLSPPSSDNFLADKWESYRKKKVVIRIGYVGTDYRGLQIQRDD 85

Query: 83  PGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYI---- 142
           P  KTIE +LE A+Y AG + + + G   +  WARS+RTDKGV ++   +S +  I    
Sbjct: 86  PSIKTIEGELEVAIYKAGGIRDSNYGDLHKIGWARSSRTDKGVHSLATSISLKMEIPETA 145

Query: 143 ---DPPGFV--DRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHR 202
              DP G V    ++ +L   IR+F        F+ ++ C  R+Y YL+PV  L      
Sbjct: 146 WKDDPQGTVLAKCISKHLPENIRVFSVLPSNRRFDPRRECTLRKYSYLLPVDVLGIKNSF 205

Query: 203 DRESVKASLGSDNELVKCLE----------CSERGRKVEGLMGKRN-------FEVKTAI 262
             + +   +   NE++K  E           S   RK E  + +RN         +K + 
Sbjct: 206 TSDEIDYHITDFNEILKEFEGEYPFHNYTQRSRYRRKSEQKIKQRNGRPPREPKSIKASE 265

HSP 2 Score: 56.6 bits (135), Expect = 5.3e-08
Identity = 29/61 (47.54%), Postives = 35/61 (57.38%), Query Frame = 1

Query: 371 GIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLY 430
           G  FV+  + G+SFMLHQIRKM+G AVA+ R   P  +I  +L K   I +P AP   L 
Sbjct: 340 GFGFVEISIWGESFMLHQIRKMIGTAVAVKRELLPRDIIRLSLNKFTRIVLPLAPSEVLI 399

Query: 431 L 432
           L
Sbjct: 400 L 400

BLAST of MELO3C017001 vs. TAIR10
Match: AT1G20290.1 (AT1G20290.1 SWI-SNF-related chromatin binding protein)

HSP 1 Score: 68.6 bits (166), Expect = 1.3e-11
Identity = 36/62 (58.06%), Postives = 39/62 (62.90%), Query Frame = 1

Query: 437 TSYNQKWKDTHEEVSMKAYEEVAED-FKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPD 496
           TS    W+    EVS+   +E   D FK K +YSHI S E KDGAVA+WLHSLN RNYPD
Sbjct: 77  TSARISWR----EVSVDDDQEQEHDQFKWKHVYSHIGSAEEKDGAVAIWLHSLNQRNYPD 134

Query: 497 LR 498
           LR
Sbjct: 137 LR 134

BLAST of MELO3C017001 vs. NCBI nr
Match: gi|659104351|ref|XP_008452911.1| (PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo])

HSP 1 Score: 1031.9 bits (2667), Expect = 3.7e-298
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 1

Query: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60
           MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240

Query: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300
           IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300

Query: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 520
           VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 519

BLAST of MELO3C017001 vs. NCBI nr
Match: gi|449455575|ref|XP_004145528.1| (PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis sativus])

HSP 1 Score: 1006.9 bits (2602), Expect = 1.3e-290
Identity = 506/519 (97.50%), Postives = 509/519 (98.07%), Query Frame = 1

Query: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60
           MEEEPPTETRRSPPQPLIEEP PKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGL+GKRNFEVKTAITESDISSN VNA
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNA 240

Query: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300
           IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATEL V SEG AKSESSEMSGLG+DSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEI 300

Query: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNGKEK SGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDAS  VVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEE AEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 520
           VALWLHSLNHRNYPDLRVIDEVAKIAKN S+DMETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQAET 519

BLAST of MELO3C017001 vs. NCBI nr
Match: gi|1012001706|ref|XP_015932762.1| (PREDICTED: tRNA pseudouridine synthase A, mitochondrial isoform X1 [Arachis duranensis])

HSP 1 Score: 698.0 bits (1800), Expect = 1.3e-197
Identity = 365/530 (68.87%), Postives = 423/530 (79.81%), Query Frame = 1

Query: 13  PPQPLI---EEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCGVGYQ 72
           PP P +   EEP+PKK KMS  T+TSDDE+  +  A   K+RYKRRK+AIFFAYCGVGYQ
Sbjct: 17  PPPPALVVTEEPEPKKQKMS--TSTSDDEECIT--ATGTKKRYKRRKIAIFFAYCGVGYQ 76

Query: 73  GMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFY 132
           GMQKNPGAKTIE D+EEALY++GAVPEQDRG++KRYDWARSARTDKGVSAVGQVVSGRFY
Sbjct: 77  GMQKNPGAKTIEGDMEEALYVSGAVPEQDRGIAKRYDWARSARTDKGVSAVGQVVSGRFY 136

Query: 133 IDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRES 192
           IDPPG VDRLNSNL  QIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDP+CHRDRE+
Sbjct: 137 IDPPGLVDRLNSNLPDQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPSCHRDRET 196

Query: 193 VKASLGSDNELVKCLECSERGRKVEGLMG--KRNFEVKTAITESDISSNIVNA-----IE 252
           V ASLGS NELVKCLECSERGRKV GL+G  K N   +    +S    + VNA     I+
Sbjct: 197 VLASLGSGNELVKCLECSERGRKVVGLVGNAKHNLTQEAMDVDSFRKDDKVNAGLTEDID 256

Query: 253 VSDVQANG------------VLEDS--ATTSDSNKQTSVLVENTATELTVASEGIAKSES 312
           VS  +  G            V+ D+  + T +S+ +  VLV+N  ++  + +    +   
Sbjct: 257 VSLSKGEGKCLNEESGHEDKVVVDNLNSKTEESSNEVKVLVDNVNSKTDLETMVPVQDAD 316

Query: 313 SEMSGLGNDSEIVVSKESVNGKEKPS--GSFKYGEEEKARFNRILKHYVGTHNFHNFTTR 372
           + ++G G+ +  +V +E VNG+++P+    F YGEEEK RFNRILK+YVGTHNFHNFTTR
Sbjct: 317 TPVNG-GSVNTAIVEEEKVNGEDRPTKGSGFNYGEEEKERFNRILKYYVGTHNFHNFTTR 376

Query: 373 TKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPE 432
           TKAEDPAARRFI+SF+A TTVVVEG+EFVKCE+VGQSFMLHQIRKM+GLAVAIMRNCAPE
Sbjct: 377 TKAEDPAARRFIISFEAKTTVVVEGMEFVKCEIVGQSFMLHQIRKMVGLAVAIMRNCAPE 436

Query: 433 SLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQ 492
           SLI KALQKDVNINVPTAPEVGLYLDEC F SYNQKWKD+HEE+SMKAYE+ AEDFKMK 
Sbjct: 437 SLINKALQKDVNINVPTAPEVGLYLDECFFASYNQKWKDSHEELSMKAYEKEAEDFKMKY 496

Query: 493 IYSHIASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQ 517
           IYSHIASTE KDG VALWLHSLNHRNYPDLR++DE A I  N   +++ +
Sbjct: 497 IYSHIASTEQKDGTVALWLHSLNHRNYPDLRLVDEEA-ITDNGKTEIKVE 540

BLAST of MELO3C017001 vs. NCBI nr
Match: gi|951015038|ref|XP_014510724.1| (PREDICTED: tRNA pseudouridine synthase A, mitochondrial isoform X1 [Vigna radiata var. radiata])

HSP 1 Score: 696.0 bits (1795), Expect = 4.8e-197
Identity = 365/526 (69.39%), Postives = 421/526 (80.04%), Query Frame = 1

Query: 3   EEPPTETRRS---PPQPLI-EEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVA 62
           E P T    S   PP PL  +EP+PKKLKMS  TTTSDDE+  +  A   K+RYKRRKVA
Sbjct: 2   ENPDTALTTSSLPPPPPLAADEPEPKKLKMS--TTTSDDEECKT--APGTKKRYKRRKVA 61

Query: 63  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 122
           IFFAYCGVGYQGMQKNPGAKTIE DLEEALY++GAVPE DRG+ KRYDWARSARTDKGVS
Sbjct: 62  IFFAYCGVGYQGMQKNPGAKTIEGDLEEALYVSGAVPEHDRGIPKRYDWARSARTDKGVS 121

Query: 123 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 182
           AVGQVVSGRFYIDPPGFVDRLNSNL  QIRIFGYKRVT SFNAKKFCDRRRYVYLIPVFA
Sbjct: 122 AVGQVVSGRFYIDPPGFVDRLNSNLPSQIRIFGYKRVTGSFNAKKFCDRRRYVYLIPVFA 181

Query: 183 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKR--NFEVKTAITESDISSN 242
           LDP+CHRDRE+V ASLGS NELVKCLECSERGRKV GL+G R  N E++    ++ ISSN
Sbjct: 182 LDPSCHRDRETVMASLGSTNELVKCLECSERGRKVVGLVGNRKHNLELEAMDVDAGISSN 241

Query: 243 ---IVNAIEVSDVQAN-GVLEDSATTSDSNKQTS--VLVENTATELTVASEGIAKSESSE 302
              +++++   DV+ +    +D     +S   T   VLV++T  E  + +    + E   
Sbjct: 242 RDVVLDSVVAEDVEVSLSRGDDDHLNGESGIDTKGEVLVDSTDPETGIENVIPVQDEDMP 301

Query: 303 MSGLGNDSEIVVSKESVNGKEKPSGS--FKYGEEEKARFNRILKHYVGTHNFHNFTTRTK 362
           ++G   ++  +V +E +NG +K +G   F YGE+E+ RFN+IL  +VGTHNFHNFTTRTK
Sbjct: 302 LNGESVNNSDIVEEEKMNGVDKSTGGSRFCYGEKERERFNKILNFFVGTHNFHNFTTRTK 361

Query: 363 AEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESL 422
           A+DPAARR+I+SF+A+TTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESL
Sbjct: 362 AQDPAARRYIISFNANTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESL 421

Query: 423 IEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIY 482
           I KALQ+DVNINVPTAPEVGLYLDEC F+SYNQKWKD+HE VSMKAYE+ AE+FKMK IY
Sbjct: 422 INKALQQDVNINVPTAPEVGLYLDECFFSSYNQKWKDSHEVVSMKAYEKEAEEFKMKYIY 481

Query: 483 SHIASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDME 515
           SHI+STE K+G VALWLHSLNHRNYPDL V++E A I  N S D E
Sbjct: 482 SHISSTEQKEGTVALWLHSLNHRNYPDLHVVNEEA-ITDNKSADPE 522

BLAST of MELO3C017001 vs. NCBI nr
Match: gi|1021473597|ref|XP_016200105.1| (PREDICTED: tRNA pseudouridine synthase A, mitochondrial isoform X1 [Arachis ipaensis])

HSP 1 Score: 693.7 bits (1789), Expect = 2.4e-196
Identity = 358/536 (66.79%), Postives = 420/536 (78.36%), Query Frame = 1

Query: 5   PPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYC 64
           PP      P   + EEP+PKK KMS  T+TSDDE+  +  A   K+RYKRRK+AIFFAYC
Sbjct: 14  PPPPPPPPPALVVTEEPEPKKQKMS--TSTSDDEECTT--ATDTKKRYKRRKIAIFFAYC 73

Query: 65  GVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVV 124
           GVGYQGMQKNPGAKTIE D+EEALY++GAVPEQDRG++KRYDWARSARTDKGVSAVGQVV
Sbjct: 74  GVGYQGMQKNPGAKTIEGDMEEALYVSGAVPEQDRGIAKRYDWARSARTDKGVSAVGQVV 133

Query: 125 SGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCH 184
           SGRFYIDPPG VDR+NSNL  QIRIFGYKRVTASFNAK+FCDRRRYVYLIPVFALDP+CH
Sbjct: 134 SGRFYIDPPGLVDRVNSNLPDQIRIFGYKRVTASFNAKRFCDRRRYVYLIPVFALDPSCH 193

Query: 185 RDRESVKASLGSDNELVKCLECSERGRKVEGLMG--KRNFEVKTAITESDISSNIVNAIE 244
           RDRE+V ASLG+ NELVKCLECSERGRKV GL+G  K N   +    +S    + VNA  
Sbjct: 194 RDRETVLASLGTGNELVKCLECSERGRKVVGLVGNAKHNLTQEAMDVDSFRKDDKVNAGL 253

Query: 245 VSDVQAN------------------GVLEDS--ATTSDSNKQTSVLVENTATELTVASEG 304
             D+  +                   V+ D+  + T +S+ +  VLV+N  ++  + +  
Sbjct: 254 TEDIDVSLSKGEEGKCLNEESGHEDKVVVDNLNSKTEESSNEVKVLVDNVNSKTDLETMV 313

Query: 305 IAKSESSEMSGLGNDSEIVVSKESVNGKEKPS--GSFKYGEEEKARFNRILKHYVGTHNF 364
             +   + ++G G+ +  +V +E VNG+++P+    F YGEEEK RFNRILK+YVGTHNF
Sbjct: 314 PVQDADTPVNG-GSVNTAIVEEEKVNGEDRPTKGSGFSYGEEEKERFNRILKYYVGTHNF 373

Query: 365 HNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIM 424
           HNFTTRTKAEDPAARRFI+SF+A TTVVVEG+EFVKCE+VGQSFMLHQIRKM+GLAVAIM
Sbjct: 374 HNFTTRTKAEDPAARRFIISFEAKTTVVVEGMEFVKCEIVGQSFMLHQIRKMVGLAVAIM 433

Query: 425 RNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAE 484
           RNCAPESLI KALQKDVNINVPTAPEVGLYLDEC F SYNQKWKD+HEE+SMKAYE+ AE
Sbjct: 434 RNCAPESLINKALQKDVNINVPTAPEVGLYLDECFFASYNQKWKDSHEELSMKAYEKEAE 493

Query: 485 DFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQ 517
           DFKMK IYSHIASTE +DG VALWLHSLNHRNYPDLR++DE A I  N   +++ +
Sbjct: 494 DFKMKYIYSHIASTEQRDGTVALWLHSLNHRNYPDLRLVDEEA-ITDNGKTEIKVE 543