ClCG05G015300 (gene) Watermelon (Charleston Gray)

NameClCG05G015300
Typegene
OrganismCitrullus lanatus (Watermelon (Charleston Gray))
DescriptiontRNA pseudouridine synthase family protein
LocationCG_Chr05 : 26895179 .. 26897220 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCTACTTCAGTCCATTCCCCTGCTTGAGTAAAATGGAAGAAGAACCGCCAACTGAAACTACAAGGTCACCGCCACAACCGTTGATCGAAGAACCCGACCCAAAGAAGCTTAAAATGTCATCCACAACCACCACCTCCGACGACGAAGATGTCAATTCCAATTCTGGGGCGCCAAAGAAACAAAGATACAAGCGCCGCAAGATCGCCATTTTCTTTGCCTACTGTGGGGTAGGTTACCAAGGGATGCAGAAGAATCCGGGCGCTAAAACCATTGAAGCTGACCTTGAAGAAGCCCTATATCTCGCCGGAGCAGTCCCGGAGCAGGACCGAGGGCTCTCCAAGCGCTACGATTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTGAGTGCCGTGGGTCAGGTTGTTTCCGGTCGCTTCTACATCGATCCGCCAGGTTTTGTTGATCGTCTCAATTCAAATCTTTCGCCTCAGATTCGAATTTTCGGGTACAAGCGCGTCACAGCGTCATTCAATGCGAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATTCCTGTATTCGCTCTCGATCCCAATTGTCATCGTGATAGAGAAAGTGTGAAAGCTAGTTTAGGATCTGACAACGAGCTTGTGAAGTGCTTAGAGTGCTCTGAACGAGGACGCAAGGTCGAAGGCTTGATGGGCAAGCGCAATTTTGAAGTAAAAACTTCAATAACTGACTCAGACATTTCTTCGAACACTGGAAAAGCCATTGAAGTTTCTGATGTTCAGGAAAATAGGGTTTTGGAGGGCAATGACACGACCTCTGATTCTAACAAACAAACTAGCGTTTTGGTTGAAAATAGTTGTACTGAACTCAATGTTGGAAGCGAAGGTACTGCAAAAAGTGAAGGCACTGAAATGTCTGGATTAGGGAATGATTCAGAGATTGTTGTAAGCAAAGAGAGCGTTAATGGGAACGAAAAACCCTCAGGGAGCTTCAAGTATGGCGAGGAGGAGAAGGCTAGATTCAATAGGATTTTGAAGAATTATGTGGGAACTCATAACTTCCATAATTTTACAACCAGAACAAAAGCAGAGGACCCTGCTGCTCGTCGGTTCATTGTTTCATTTGATGCGTCTACCACTGTCGTGGTTGAAGGCATCGAATTTGTGAAATGTGAAGTTGTGGGGCAGAGCTTCATGCTTCATCAGATCCGAAAGATGATGGGTCTTGCTGTGGCAATCATGAGGAACTGTGCTCCAGAGTCATTGATTGAGAAGGCTTTGCAAAAGTAAGTAATTTCCATTTTCAACTACAATTTGATTTGATTTTCATTTGTTTAATAGAATTGAATTGGATTCAAGTAATCATAATTCAAACTACAGGGATGTAAATATCAATGTGCCCACTGCACCTGAGGTTGGGCTGTACTTAGATGAGTGCCTATTCACATCGTATAATCAAAAGTGGAAAGACAGTCATGAAGAGGTATCTATGAAAGCATACGAAGAGGTAGCACAAGATTTCAAAATGAAGCAAATATATTCACACATAGCTTCCACAGAGCACAAAGATGGAGCCGTGGCCTTATGGTTGCATTCTCTAAACCATCGGAATTATCCAGATTTACGGGTTGTTGATGAGGTTGCAGAAATTGCTAAGAACACAAGTGTTGCCATGGAGACACAAGGCTGAAACTTAAAAGGTCTTCTTCCACCTTTGCTTTAACCTTCCATTTGGATGTTTTGAGCGAAAAATATTTATTTTTTATGATTTAGTGCTTGTTTAATTATTACTCTAATCAGATTATGCATAAATTTTGTTTAAGAAAGTGAGGCAAATCCATTAATTATGTTAAGTTCTGATTACTATCAAGCTCCCCAAACCCCCTCAATGAGTCATGTTATCCTTACATACCCCTGCTACAAGGGTTTGAAAAAAAAAATTGTTTACCATATGATCAACAAGGGTGGTTGTAACTTCTAGGAATACTAGATGTAAGAGAGAGGAACTCAGAAATACTTTTTTGTCCATGCATCTATGAAGAATATCTGCA

mRNA sequence

GTCTACTTCAGTCCATTCCCCTGCTTGAGTAAAATGGAAGAAGAACCGCCAACTGAAACTACAAGGTCACCGCCACAACCGTTGATCGAAGAACCCGACCCAAAGAAGCTTAAAATGTCATCCACAACCACCACCTCCGACGACGAAGATGTCAATTCCAATTCTGGGGCGCCAAAGAAACAAAGATACAAGCGCCGCAAGATCGCCATTTTCTTTGCCTACTGTGGGGTAGGTTACCAAGGGATGCAGAAGAATCCGGGCGCTAAAACCATTGAAGCTGACCTTGAAGAAGCCCTATATCTCGCCGGAGCAGTCCCGGAGCAGGACCGAGGGCTCTCCAAGCGCTACGATTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTGAGTGCCGTGGGTCAGGTTGTTTCCGGTCGCTTCTACATCGATCCGCCAGGTTTTGTTGATCGTCTCAATTCAAATCTTTCGCCTCAGATTCGAATTTTCGGGTACAAGCGCGTCACAGCGTCATTCAATGCGAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATTCCTGTATTCGCTCTCGATCCCAATTGTCATCGTGATAGAGAAAGTGTGAAAGCTAGTTTAGGATCTGACAACGAGCTTGTGAAGTGCTTAGAGTGCTCTGAACGAGGACGCAAGGTCGAAGGCTTGATGGGCAAGCGCAATTTTGAAGTAAAAACTTCAATAACTGACTCAGACATTTCTTCGAACACTGGAAAAGCCATTGAAGTTTCTGATGTTCAGGAAAATAGGGTTTTGGAGGGCAATGACACGACCTCTGATTCTAACAAACAAACTAGCGTTTTGGTTGAAAATAGTTGTACTGAACTCAATGTTGGAAGCGAAGGTACTGCAAAAAGTGAAGGCACTGAAATGTCTGGATTAGGGAATGATTCAGAGATTGTTGTAAGCAAAGAGAGCGTTAATGGGAACGAAAAACCCTCAGGGAGCTTCAAGTATGGCGAGGAGGAGAAGGCTAGATTCAATAGGATTTTGAAGAATTATGTGGGAACTCATAACTTCCATAATTTTACAACCAGAACAAAAGCAGAGGACCCTGCTGCTCGTCGGTTCATTGTTTCATTTGATGCGTCTACCACTGTCGTGGTTGAAGGCATCGAATTTGTGAAATGTGAAGTTGTGGGGCAGAGCTTCATGCTTCATCAGATCCGAAAGATGATGGGTCTTGCTGTGGCAATCATGAGGAACTGTGCTCCAGAGTCATTGATTGAGAAGGCTTTGCAAAAGGATGTAAATATCAATGTGCCCACTGCACCTGAGGTTGGGCTGTACTTAGATGAGTGCCTATTCACATCGTATAATCAAAAGTGGAAAGACAGTCATGAAGAGGTATCTATGAAAGCATACGAAGAGGTAGCACAAGATTTCAAAATGAAGCAAATATATTCACACATAGCTTCCACAGAGCACAAAGATGGAGCCGTGGCCTTATGGTTGCATTCTCTAAACCATCGGAATTATCCAGATTTACGGGTTGTTGATGAGGTTGCAGAAATTGCTAAGAACACAAGTGTTGCCATGGAGACACAAGGCTGAAACTTAAAAGGTCTTCTTCCACCTTTGCTTTAACCTTCCATTTGGATGTTTTGAGCGAAAAATATTTATTTTTTATGATTTAGTGCTTGTTTAATTATTACTCTAATCAGATTATGCATAAATTTTGTTTAAGAAAGTGAGGCAAATCCATTAATTATGTTAAGTTCTGATTACTATCAAGCTCCCCAAACCCCCTCAATGAGTCATGTTATCCTTACATACCCCTGCTACAAGGGTTTGAAAAAAAAAATTGTTTACCATATGATCAACAAGGGTGGTTGTAACTTCTAGGAATACTAGATGTAAGAGAGAGGAACTCAGAAATACTTTTTTGTCCATGCATCTATGAAGAATATCTGCA

Coding sequence (CDS)

ATGGAAGAAGAACCGCCAACTGAAACTACAAGGTCACCGCCACAACCGTTGATCGAAGAACCCGACCCAAAGAAGCTTAAAATGTCATCCACAACCACCACCTCCGACGACGAAGATGTCAATTCCAATTCTGGGGCGCCAAAGAAACAAAGATACAAGCGCCGCAAGATCGCCATTTTCTTTGCCTACTGTGGGGTAGGTTACCAAGGGATGCAGAAGAATCCGGGCGCTAAAACCATTGAAGCTGACCTTGAAGAAGCCCTATATCTCGCCGGAGCAGTCCCGGAGCAGGACCGAGGGCTCTCCAAGCGCTACGATTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTGAGTGCCGTGGGTCAGGTTGTTTCCGGTCGCTTCTACATCGATCCGCCAGGTTTTGTTGATCGTCTCAATTCAAATCTTTCGCCTCAGATTCGAATTTTCGGGTACAAGCGCGTCACAGCGTCATTCAATGCGAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATTCCTGTATTCGCTCTCGATCCCAATTGTCATCGTGATAGAGAAAGTGTGAAAGCTAGTTTAGGATCTGACAACGAGCTTGTGAAGTGCTTAGAGTGCTCTGAACGAGGACGCAAGGTCGAAGGCTTGATGGGCAAGCGCAATTTTGAAGTAAAAACTTCAATAACTGACTCAGACATTTCTTCGAACACTGGAAAAGCCATTGAAGTTTCTGATGTTCAGGAAAATAGGGTTTTGGAGGGCAATGACACGACCTCTGATTCTAACAAACAAACTAGCGTTTTGGTTGAAAATAGTTGTACTGAACTCAATGTTGGAAGCGAAGGTACTGCAAAAAGTGAAGGCACTGAAATGTCTGGATTAGGGAATGATTCAGAGATTGTTGTAAGCAAAGAGAGCGTTAATGGGAACGAAAAACCCTCAGGGAGCTTCAAGTATGGCGAGGAGGAGAAGGCTAGATTCAATAGGATTTTGAAGAATTATGTGGGAACTCATAACTTCCATAATTTTACAACCAGAACAAAAGCAGAGGACCCTGCTGCTCGTCGGTTCATTGTTTCATTTGATGCGTCTACCACTGTCGTGGTTGAAGGCATCGAATTTGTGAAATGTGAAGTTGTGGGGCAGAGCTTCATGCTTCATCAGATCCGAAAGATGATGGGTCTTGCTGTGGCAATCATGAGGAACTGTGCTCCAGAGTCATTGATTGAGAAGGCTTTGCAAAAGGATGTAAATATCAATGTGCCCACTGCACCTGAGGTTGGGCTGTACTTAGATGAGTGCCTATTCACATCGTATAATCAAAAGTGGAAAGACAGTCATGAAGAGGTATCTATGAAAGCATACGAAGAGGTAGCACAAGATTTCAAAATGAAGCAAATATATTCACACATAGCTTCCACAGAGCACAAAGATGGAGCCGTGGCCTTATGGTTGCATTCTCTAAACCATCGGAATTATCCAGATTTACGGGTTGTTGATGAGGTTGCAGAAATTGCTAAGAACACAAGTGTTGCCATGGAGACACAAGGCTGA

Protein sequence

MEEEPPTETTRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSGAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSNTGKAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSGLGNDSEIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQG
BLAST of ClCG05G015300 vs. Swiss-Prot
Match: PUS1_SCHPO (tRNA pseudouridine synthase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=pus1 PE=1 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 3.4e-49
Identity = 151/498 (30.32%), Postives = 229/498 (45.98%), Query Frame = 1

Query: 50  QRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWAR 109
           ++  +RK+A    YCG GY GMQ NP +KTIE DL +A   AGAV   +    K+   AR
Sbjct: 39  EKKPKRKVACLVGYCGSGYHGMQLNPPSKTIEGDLFDAFVKAGAVSSYNADDPKKVALAR 98

Query: 110 SARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRR 169
           +ARTDKGV A G V+S +  ++    ++++N +L P IR++   R   SFN + +C+ R 
Sbjct: 99  AARTDKGVHAAGNVISLKLIMEDEKLIEKVNEHLPPSIRLWDVIRTINSFNPRTYCESRI 158

Query: 170 YVYLIPVFALDP--------NCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRN 229
           Y Y++P +A  P        NC      + A      E +     ++  R +    GK  
Sbjct: 159 YEYMVPTYAFVPPKPSSILGNCIMKNSPMPA------EPINKENINQLSRSLFYEEGKEF 218

Query: 230 FEVKTSITDSDISSNTGKAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSE 289
           ++      D DI+     A E+  + E    +     +  +K+ +  + NS  E N GSE
Sbjct: 219 WD------DYDIA-----AKEILSLYEQ---DPEGFVNPYSKRGAAALANS--ENNKGSE 278

Query: 290 -GTAKSEGTEMSGLGNDSEIVVSK---------ESVNGNEKPS----GSFKYGEEEKARF 349
            G +     +M    +DS  +V++         ES      PS     + K+ E  K + 
Sbjct: 279 AGVSAKTNPDMD---SDSSAIVNEFLKPDSVEDESAGSKIDPSYRLERALKHIEVLKLKN 338

Query: 350 NRI-----------LKNYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVK 409
            RI           L  YVG HNFHNFT         + R I SF AS   ++   E++ 
Sbjct: 339 YRISADRLSVIRETLNQYVGVHNFHNFTVGQAFHQKNSNRVIRSFTASDPFMIGDTEWIS 398

Query: 410 CEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLF 469
           C+V GQSFMLHQIRKM+ LA+ ++R   P   I+ A +K   IN+P  P  GL L+   F
Sbjct: 399 CKVHGQSFMLHQIRKMIALAILVVRTGCPVERIQDAFKK-TKINIPKGPGFGLLLESPFF 458

Query: 470 TSYNQKW--KDSHEEVSMKAYEEVAQDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYP 513
             YN+    +++ + +    YE+    FK   IY  I   E +      +L  ++  N  
Sbjct: 459 KGYNEHKAPENNRDPIDFTKYEQKITAFKHAHIYDKIFLEEARKQVFHCFLSFIDSYNEE 510

BLAST of ClCG05G015300 vs. Swiss-Prot
Match: PUS1_CANAL (tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=PUS1 PE=3 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 8.3e-40
Identity = 116/377 (30.77%), Postives = 180/377 (47.75%), Query Frame = 1

Query: 103 KRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAK 162
           K+  + R+ARTDKGV A G V+S +  I+ P  ++++N  L  QIRI+G +R T  F+ +
Sbjct: 37  KKSGFQRAARTDKGVHAAGNVISLKMIIEDPEIINKINDLLPKQIRIWGIQRATKGFDCR 96

Query: 163 KFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNF 222
           K C  R Y YL+P F+L P      +SV + L  + EL       +    ++     ++ 
Sbjct: 97  KCCSSRIYEYLLPTFSLLP---PKPKSVLSELVKEKELENPDLFEDDQEGIDWWENVKSK 156

Query: 223 EVKTSITDSDISSNTGKAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEG 282
            + + IT   I S T      S   E +  + +     ++++   + E S T+L      
Sbjct: 157 ILASGITQEQIDSIT------SSYDEQQQQQHHQQQQQADEEERDISELSFTKL------ 216

Query: 283 TAKSEGTEMSGLGNDSEIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHN 342
             K   T            +  +S       S   ++  E        +K Y GTHNFHN
Sbjct: 217 -IKQIKT------------IENQSRRSYRISSSRLQHFREV-------MKQYEGTHNFHN 276

Query: 343 FTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRN 402
           FT     +D +A RF++    S   V+EG E++  ++ GQSFMLHQIRKM+ +A  ++R 
Sbjct: 277 FTVGKPFKDTSANRFMIKTIVSDPFVIEGTEWISIKIHGQSFMLHQIRKMIAMAALVVRL 336

Query: 403 CAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQK-WKDSHEEVSMKAYEEVAQD 462
             P  +I    Q    IN+P AP +GL L+  +F  YN K  K  +E +    +++   +
Sbjct: 337 SLPCGIINNFFQ-STKINIPKAPALGLLLENPVFDGYNIKLTKSDYEPIDFTKFDKEMNE 377

Query: 463 FKMKQIYSHIASTEHKD 479
           FKMK IY  I + E K+
Sbjct: 397 FKMKYIYDKIYAEESKE 377

BLAST of ClCG05G015300 vs. Swiss-Prot
Match: TRUA_MOUSE (tRNA pseudouridine synthase A, mitochondrial OS=Mus musculus GN=Pus1 PE=1 SV=2)

HSP 1 Score: 138.3 bits (347), Expect = 2.4e-31
Identity = 74/180 (41.11%), Postives = 108/180 (60.00%), Query Frame = 1

Query: 316 SFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFV 375
           S++   E   + NR+L  Y GTHNFHNFT++    +P+ARR+I+        V EG+EF 
Sbjct: 217 SYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFA 276

Query: 376 KCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECL 435
             +V GQSFM+HQIRKM+GL VAI++  APES++E++  ++  ++VP AP +GL L+   
Sbjct: 277 VIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEE-KVDVPKAPGLGLVLERVH 336

Query: 436 FTSYNQKWKDS--HEEVSMKAYEEVAQDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNY 494
           F  YNQ++     HE +     E     FK + IY  I STE  + ++A WL++L   N+
Sbjct: 337 FEKYNQRFGGDGLHEPLDWTQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNF 395


HSP 2 Score: 123.2 bits (308), Expect = 8.1e-27
Identity = 79/210 (37.62%), Postives = 108/210 (51.43%), Query Frame = 1

Query: 5   PPTETTRSPPQPLIEEPDPK-KLKMSSTTTTSDDEDVNSNSGAPKKQRYKRRKIAIFFAY 64
           PP    + PP     +PD K +       T    + V         ++  +RKI +  AY
Sbjct: 29  PPMAGNKVPPALASHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPRKLPKRKIVLLMAY 88

Query: 65  CGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 124
            G GY GMQ+N G+   +TIE DL  AL  AG +PE      ++  + R ARTDKGVSA 
Sbjct: 89  SGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAA 148

Query: 125 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 184
           GQVVS + ++     +D++NS+L   IRI G KRVT  FN+K  CD R Y Y++P FA  
Sbjct: 149 GQVVSLKVWLIDD-ILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAF- 208

Query: 185 PNCHRDR----ESVKASLGSDNELVKCLEC 207
              H+DR    ES + S  +  ++ + L C
Sbjct: 209 --AHKDRDVQDESYRLSAETLQQVNRLLAC 234

BLAST of ClCG05G015300 vs. Swiss-Prot
Match: TRUA_RAT (tRNA pseudouridine synthase A, mitochondrial OS=Rattus norvegicus GN=Pus1 PE=1 SV=2)

HSP 1 Score: 138.3 bits (347), Expect = 2.4e-31
Identity = 74/180 (41.11%), Postives = 108/180 (60.00%), Query Frame = 1

Query: 316 SFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFV 375
           S++   E   + NR+L  Y GTHNFHNFT++    +P+ARR+I+        V EG+EF 
Sbjct: 217 SYRLSAETLQQVNRLLGCYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFA 276

Query: 376 KCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECL 435
             +V GQSFM+HQIRKM+GL VAI++  APES++E++  ++  ++VP AP +GL L+   
Sbjct: 277 VIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEE-KVDVPKAPGLGLVLERVH 336

Query: 436 FTSYNQKWKDS--HEEVSMKAYEEVAQDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNY 494
           F  YNQ++     HE +     E     FK + IY  I STE  + ++A WL++L   N+
Sbjct: 337 FEKYNQRFGSDGLHEPLDWAQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNF 395


HSP 2 Score: 122.1 bits (305), Expect = 1.8e-26
Identity = 79/210 (37.62%), Postives = 108/210 (51.43%), Query Frame = 1

Query: 5   PPTETTRSPPQPLIEEPDPK-KLKMSSTTTTSDDEDVNSNSGAPKKQRYKRRKIAIFFAY 64
           PP    + PP     +PD K +       T    + V         ++  +RKI +  AY
Sbjct: 29  PPMAGNKVPPALPSHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPRKLPKRKIVLLMAY 88

Query: 65  CGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 124
            G GY GMQ+N G+   +TIE DL  AL  AG +PE      ++  + R ARTDKGVSA 
Sbjct: 89  SGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAA 148

Query: 125 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 184
           GQVVS + ++     +D++NS+L   IRI G KRVT  FN+K  CD R Y Y++P FA  
Sbjct: 149 GQVVSLKVWLIDD-ILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAF- 208

Query: 185 PNCHRDR----ESVKASLGSDNELVKCLEC 207
              H+DR    ES + S  +  ++ + L C
Sbjct: 209 --AHKDRDVQDESYRLSAETLQQVNRLLGC 234

BLAST of ClCG05G015300 vs. Swiss-Prot
Match: TRUA_HUMAN (tRNA pseudouridine synthase A, mitochondrial OS=Homo sapiens GN=PUS1 PE=1 SV=3)

HSP 1 Score: 133.7 bits (335), Expect = 6.0e-30
Identity = 72/180 (40.00%), Postives = 105/180 (58.33%), Query Frame = 1

Query: 316 SFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFV 375
           +++   E   + NR+L  Y GTHNFHNFT++   +DP+A R+I+        V EG+EF 
Sbjct: 221 TYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFA 280

Query: 376 KCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECL 435
              V GQSFM+HQIRKM+GL VAI++  APES++E++   +  ++VP AP +GL L+   
Sbjct: 281 VIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTE-KVDVPKAPGLGLVLERVH 340

Query: 436 FTSYNQKWKDS--HEEVSMKAYEEVAQDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNY 494
           F  YNQ++ +   HE +     E     FK + IY  I  TE  + ++A WL +L   N+
Sbjct: 341 FEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNF 399


HSP 2 Score: 120.6 bits (301), Expect = 5.2e-26
Identity = 69/150 (46.00%), Postives = 91/150 (60.67%), Query Frame = 1

Query: 44  SGAPKKQRYK--RRKIAIFFAYCGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEQD 103
           SG  +++R K  +RKI +  AY G GY GMQ+N G+   KTIE DL  AL  +G +PE  
Sbjct: 71  SGGDEERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENH 130

Query: 104 RGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTAS 163
               ++  + R ARTDKGVSA GQVVS + ++     ++++NS+L   IRI G KRVT  
Sbjct: 131 GEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDD-ILEKINSHLPSHIRILGLKRVTGG 190

Query: 164 FNAKKFCDRRRYVYLIPVFALDPNCHRDRE 189
           FN+K  CD R Y YL+P FA     H+DR+
Sbjct: 191 FNSKNRCDARTYCYLLPTFAF---AHKDRD 216

BLAST of ClCG05G015300 vs. TrEMBL
Match: E5GCD2_CUCME (tRNA pseudouridine synthase family protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 978.4 bits (2528), Expect = 3.4e-282
Identity = 489/516 (94.77%), Postives = 500/516 (96.90%), Query Frame = 1

Query: 1   MEEEPPTETTRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSGAPKKQRYKRRKIAIF 60
           MEEEPPTET RSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNS APKKQRYKRRK+AIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSNTGKA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKT+IT+SDISSN   A
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240

Query: 241 IEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSGLGNDSEI 300
           IEVSDVQ N VLE + TTSDSNKQTSVLVEN+ TEL V SEG AKSE +EMSGLGNDSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300

Query: 301 VVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNG EKPSGSFKYGEEEKARFNRILK+YVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEEVA+DFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQ 517
           VALWLHSLNHRNYPDLRV+DEVA+IAKNTSV METQ
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQ 516

BLAST of ClCG05G015300 vs. TrEMBL
Match: A0A0A0L0J9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G652870 PE=4 SV=1)

HSP 1 Score: 969.9 bits (2506), Expect = 1.2e-279
Identity = 484/516 (93.80%), Postives = 498/516 (96.51%), Query Frame = 1

Query: 1   MEEEPPTETTRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSGAPKKQRYKRRKIAIF 60
           MEEEPPTET RSPPQPLIEEP PKKLKMSSTTTTSDDEDVNSNS APKKQRYKRRK+AIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSNTGKA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGL+GKRNFEVKT+IT+SDISSNT  A
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNA 240

Query: 241 IEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSGLGNDSEI 300
           IEVSDVQ N VLE + TTSDSNKQTSVLVEN+ TELNVGSEGTAKSE +EMSGLG+DSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEI 300

Query: 301 VVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNG EK SGSFKYGEEEKARFNRILK+YVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDAS  VVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEE A+DFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQ 517
           VALWLHSLNHRNYPDLRV+DEVA+IAKN S+ METQ
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQ 516

BLAST of ClCG05G015300 vs. TrEMBL
Match: A0A0L9TQX3_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g245700 PE=4 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 1.3e-196
Identity = 356/513 (69.40%), Postives = 414/513 (80.70%), Query Frame = 1

Query: 3   EEPPTETTRS---PPQPLI-EEPDPKKLKMSSTTTTSDDEDVNSNSGAPKKQRYKRRKIA 62
           E P T  T S   PP PL  +EP+PKKLKMS  TTTSDDE+  +  G   K+RYKRRK+A
Sbjct: 2   ENPDTALTTSSLPPPPPLAADEPEPKKLKMS--TTTSDDEECKTAPGT--KKRYKRRKVA 61

Query: 63  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 122
           IFFAYCGVGYQGMQKNPGAKTIE DLEEALY++GAVPE DRG+ KRYDWARSARTDKGVS
Sbjct: 62  IFFAYCGVGYQGMQKNPGAKTIEGDLEEALYVSGAVPEHDRGIPKRYDWARSARTDKGVS 121

Query: 123 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 182
           AVGQVVSGRFYIDPPG VDRLNSNL  QIRIFGYKRVT SF+AKKFCDRRRYVYLIPVFA
Sbjct: 122 AVGQVVSGRFYIDPPGLVDRLNSNLPSQIRIFGYKRVTGSFSAKKFCDRRRYVYLIPVFA 181

Query: 183 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSNTG 242
           LDP+CHRDRE+V ASLGS NELVKCLECSERGRKV GL+G R   ++    D D  SN  
Sbjct: 182 LDPSCHRDRETVMASLGSTNELVKCLECSERGRKVVGLVGNRKHNLELEAMDVDAGSNRD 241

Query: 243 KAIE--VSDVQENRVLEGNDT----TSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMS 302
             ++  V++  E  + +G+D      S  + +  VLV+ +  E  + +  T + E   ++
Sbjct: 242 VVLDSVVAEDVEVSLSKGDDNHLNGESGIDTKGEVLVDGTNPETGIENMITVQDEDMPLN 301

Query: 303 GLGNDSEIVVSKESVNGNEKPSGS--FKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAE 362
           G   ++  ++ +E +NG +K +G   F YGE+E+ RFN++L  +VGTHNFHNFTTRTKA+
Sbjct: 302 GESVNNSDIIEEEKMNGVDKSTGGSRFCYGEKERERFNKVLNFFVGTHNFHNFTTRTKAQ 361

Query: 363 DPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIE 422
           DPAARR+I+SF+A+TTVVVEGIEFVKCEVVGQSFMLHQIRKM+GLAVAIMRNCAPESLI 
Sbjct: 362 DPAARRYIISFNANTTVVVEGIEFVKCEVVGQSFMLHQIRKMIGLAVAIMRNCAPESLIN 421

Query: 423 KALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSH 482
           KALQ+DVNINVPTAPEVGLYLDEC F+SYNQKWKDSHEEVSMKAYE+ A++FKMK IYSH
Sbjct: 422 KALQQDVNINVPTAPEVGLYLDECFFSSYNQKWKDSHEEVSMKAYEKEAEEFKMKYIYSH 481

Query: 483 IASTEHKDGAVALWLHSLNHRNYPDLRVVDEVA 504
           I+STE K+G VALWLHSLNHRNYPDLRVV+E A
Sbjct: 482 ISSTEQKEGTVALWLHSLNHRNYPDLRVVNEEA 510

BLAST of ClCG05G015300 vs. TrEMBL
Match: A0A0S3R6B4_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G417100 PE=4 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 1.3e-196
Identity = 356/513 (69.40%), Postives = 414/513 (80.70%), Query Frame = 1

Query: 3   EEPPTETTRS---PPQPLI-EEPDPKKLKMSSTTTTSDDEDVNSNSGAPKKQRYKRRKIA 62
           E P T  T S   PP PL  +EP+PKKLKMS  TTTSDDE+  +  G   K+RYKRRK+A
Sbjct: 2   ENPDTALTTSSLPPPPPLAADEPEPKKLKMS--TTTSDDEECKTAPGT--KKRYKRRKVA 61

Query: 63  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 122
           IFFAYCGVGYQGMQKNPGAKTIE DLEEALY++GAVPE DRG+ KRYDWARSARTDKGVS
Sbjct: 62  IFFAYCGVGYQGMQKNPGAKTIEGDLEEALYVSGAVPEHDRGIPKRYDWARSARTDKGVS 121

Query: 123 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 182
           AVGQVVSGRFYIDPPG VDRLNSNL  QIRIFGYKRVT SF+AKKFCDRRRYVYLIPVFA
Sbjct: 122 AVGQVVSGRFYIDPPGLVDRLNSNLPSQIRIFGYKRVTGSFSAKKFCDRRRYVYLIPVFA 181

Query: 183 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSNTG 242
           LDP+CHRDRE+V ASLGS NELVKCLECSERGRKV GL+G R   ++    D D  SN  
Sbjct: 182 LDPSCHRDRETVMASLGSTNELVKCLECSERGRKVVGLVGNRKHNLELEAMDVDAGSNRD 241

Query: 243 KAIE--VSDVQENRVLEGNDT----TSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMS 302
             ++  V++  E  + +G+D      S  + +  VLV+ +  E  + +  T + E   ++
Sbjct: 242 VVLDSVVAEDVEVSLSKGDDNHLNGESGIDTKGEVLVDGTNPETGIENMITVQDEDMPLN 301

Query: 303 GLGNDSEIVVSKESVNGNEKPSGS--FKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAE 362
           G   ++  ++ +E +NG +K +G   F YGE+E+ RFN++L  +VGTHNFHNFTTRTKA+
Sbjct: 302 GESVNNSDIIEEEKMNGVDKSTGGSRFCYGEKERERFNKVLNFFVGTHNFHNFTTRTKAQ 361

Query: 363 DPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIE 422
           DPAARR+I+SF+A+TTVVVEGIEFVKCEVVGQSFMLHQIRKM+GLAVAIMRNCAPESLI 
Sbjct: 362 DPAARRYIISFNANTTVVVEGIEFVKCEVVGQSFMLHQIRKMIGLAVAIMRNCAPESLIN 421

Query: 423 KALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSH 482
           KALQ+DVNINVPTAPEVGLYLDEC F+SYNQKWKDSHEEVSMKAYE+ A++FKMK IYSH
Sbjct: 422 KALQQDVNINVPTAPEVGLYLDECFFSSYNQKWKDSHEEVSMKAYEKEAEEFKMKYIYSH 481

Query: 483 IASTEHKDGAVALWLHSLNHRNYPDLRVVDEVA 504
           I+STE K+G VALWLHSLNHRNYPDLRVV+E A
Sbjct: 482 ISSTEQKEGTVALWLHSLNHRNYPDLRVVNEEA 510

BLAST of ClCG05G015300 vs. TrEMBL
Match: A0A0B2S802_GLYSO (tRNA pseudouridine synthase 1 OS=Glycine soja GN=glysoja_036980 PE=4 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 2.4e-195
Identity = 362/518 (69.88%), Postives = 418/518 (80.69%), Query Frame = 1

Query: 1   MEEEPPTETTRS-PPQPLI--EEPDPKKLKMSSTTTTSDDEDVNSNSGAPKKQRYKRRKI 60
           ME    T T+ S PP P +  EEP+PKKLKMS  TTTSDDE+  + +G   K+RYKRRK+
Sbjct: 1   MENSDTTLTSSSLPPPPPVSAEEPEPKKLKMS--TTTSDDEECIAGTGT--KKRYKRRKV 60

Query: 61  AIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGV 120
           AIFFAYCGVGYQGMQKNPGAKTIE DLEEALY++GAVPEQDRG+ KRYDWARSARTDKGV
Sbjct: 61  AIFFAYCGVGYQGMQKNPGAKTIEGDLEEALYVSGAVPEQDRGVPKRYDWARSARTDKGV 120

Query: 121 SAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVF 180
           SAVGQVVSGRFYIDPPG VDRLNSNL  QIRIFGYKRVTASFNAKKFCDRRRYVYLIPVF
Sbjct: 121 SAVGQVVSGRFYIDPPGLVDRLNSNLPSQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVF 180

Query: 181 ALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMG--KRNFEVKTSITDSDISS 240
           ALDP+CHRDRE+V ASLGS NELVKCLECSERGRKV GL+G  K+N E++    ++ ISS
Sbjct: 181 ALDPSCHRDRETVMASLGSGNELVKCLECSERGRKVVGLVGNRKQNLELEAVDVEAGISS 240

Query: 241 NTGKA------IEVSDVQENRVLE--GNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSE 300
           N          + +S   +N + E  GND     N +T +      T + V      + E
Sbjct: 241 NRDSGVTEDVEVSLSKGDDNHLNEESGNDNKGGMNCKTGLE-----TVIPV------QEE 300

Query: 301 GTEMSGLGNDSEIVVSKESVNGNEKPSGS--FKYGEEEKARFNRILKNYVGTHNFHNFTT 360
           GT ++G   ++  ++ +E VNG +KP+    F YGE+E+ RFN+ILK +VGTHNFHNFTT
Sbjct: 301 GTPLNGESVNNSDILEEEKVNGEDKPTNGSRFCYGEKERERFNKILKCFVGTHNFHNFTT 360

Query: 361 RTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAP 420
           RTKAEDPAARR+I+SF+A+TT+VVEG+EFVKCEVVGQSFMLHQIRKM+GLAVAIMRNCAP
Sbjct: 361 RTKAEDPAARRYIISFNANTTLVVEGVEFVKCEVVGQSFMLHQIRKMIGLAVAIMRNCAP 420

Query: 421 ESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMK 480
           ESLI++ALQ+DVNINVPTAPEVGLYLDEC FTSYNQKWKD HEE+SMKAYE+ A +FKMK
Sbjct: 421 ESLIDEALQQDVNINVPTAPEVGLYLDECFFTSYNQKWKD-HEELSMKAYEKEADEFKMK 480

Query: 481 QIYSHIASTEHKDGAVALWLHSLNHRNYPDLRVVDEVA 504
            IYSHIASTE K+G VALWLHSLNHRNYPDL V++E A
Sbjct: 481 YIYSHIASTEQKEGTVALWLHSLNHRNYPDLHVINEEA 502

BLAST of ClCG05G015300 vs. TAIR10
Match: AT1G20370.1 (AT1G20370.1 Pseudouridine synthase family protein)

HSP 1 Score: 555.4 bits (1430), Expect = 3.6e-158
Identity = 297/489 (60.74%), Postives = 359/489 (73.42%), Query Frame = 1

Query: 20  EPDPKKLKMSSTTTTSDDEDVN---SNSGAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPG 79
           EPD KKLK+S+T   +D   V    SN    +  +Y+RRK+AI FA+CGVGYQGMQKNPG
Sbjct: 20  EPDHKKLKISTTGQETDSSAVTTGGSNVAERRAPKYRRRKVAIVFAFCGVGYQGMQKNPG 79

Query: 80  AKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFV 139
           AKTIE +LEEAL+ AGAVP+ DR   + Y+WARSARTDKGVSAVGQVVSGRFY+DPPGFV
Sbjct: 80  AKTIEGELEEALFHAGAVPDADRNKPRNYEWARSARTDKGVSAVGQVVSGRFYVDPPGFV 139

Query: 140 DRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGS 199
           +RLNS L  QIR+FGYKRV  SF++KKFCDRRRYVYLIPVFALDP  H + E V+  LG 
Sbjct: 140 ERLNSKLPDQIRVFGYKRVAPSFSSKKFCDRRRYVYLIPVFALDPCVHGEAEMVRTDLGY 199

Query: 200 DNELVKCLECSERGRKVE-GLMGKRN--FEVKTSITDSDISSNTGKAIEVSDVQENRVLE 259
             E VKC+ECSE+G K+  G+MGK     + K+    SDISS+T  AI  +DV+    L 
Sbjct: 200 --EYVKCVECSEKGYKIPLGVMGKDTDCCDTKSLEIQSDISSSTCDAIG-ADVK-CETLS 259

Query: 260 GNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSGLGNDSEIVVSKESVNGNE-- 319
            +  ++++N  + VL     + L+      +K+EG E S         ++K  +N  E  
Sbjct: 260 SSVPSAENNLNSEVLDGADVSALS------SKAEGMEESN-------TLAKVEMNNGEGE 319

Query: 320 ---KPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVV 379
              K    F YGE+EK RFNRIL  YVG+HNFHNFTTRTKA DPAA R+I+SF+A+T + 
Sbjct: 320 DMTKSESKFCYGEKEKERFNRILSYYVGSHNFHNFTTRTKAADPAANRYILSFNANTVIN 379

Query: 380 VEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVG 439
           ++G +FVKCEVVGQSFMLHQIRKM+GLAVAIMRN APESLIE + +KDV INVP APEVG
Sbjct: 380 LDGKDFVKCEVVGQSFMLHQIRKMIGLAVAIMRNYAPESLIEASFKKDVRINVPMAPEVG 439

Query: 440 LYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSHIASTEHKDGAVALWLHSL 498
           LYLDEC FTSYN+++K SHEEVSM+ Y+EVA++FK K +YSHI S E KDGAVA+WLHSL
Sbjct: 440 LYLDECFFTSYNKRFKGSHEEVSMEEYKEVAEEFKWKYVYSHIGSAEEKDGAVAIWLHSL 491

BLAST of ClCG05G015300 vs. TAIR10
Match: AT1G76120.1 (AT1G76120.1 Pseudouridine synthase family protein)

HSP 1 Score: 535.8 bits (1379), Expect = 2.9e-152
Identity = 285/470 (60.64%), Postives = 337/470 (71.70%), Query Frame = 1

Query: 31  TTTTSDDEDVNSNSGAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYL 90
           TTTT ++ D++ + G  + Q     K+AI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ 
Sbjct: 3   TTTTDEEIDLSCDGGVTEPQ-----KVAIIFAFCGVGYQGMQKNPGAKTIEGELEEALFH 62

Query: 91  AGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIF 150
           AGAVPE  RG  K YD+ARSARTDKGVSAVGQVVSGRF +DP GFV+RLNSNL  QIRIF
Sbjct: 63  AGAVPESIRGKPKLYDFARSARTDKGVSAVGQVVSGRFIVDPLGFVNRLNSNLPNQIRIF 122

Query: 151 GYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECSERG 210
           GYK VT SF++KKFCDRRRYVYL+PVFALDP  HRDRE+V ASLG   E VKC ECSERG
Sbjct: 123 GYKHVTPSFSSKKFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERG 182

Query: 211 RKVE-GLMGK---RNFEVKTSITDSDISSNTGKAIEVSDVQENRVLEGNDTTSDSNKQTS 270
           RK+  GL+GK    NF  K+    SDISSN   A+  SD++   +          + +  
Sbjct: 183 RKIPPGLVGKWKGTNFGTKSLDFQSDISSNNSSALR-SDIKIEALSSNLAGLCSVDVEVG 242

Query: 271 VLVENSCTELNVGSEGTAKSEGTEMSGLGNDSEIVVSKESVNGNEKPSGSFKYGEEEKAR 330
            + E+SC +LN  S  T                            K    F YGE+EK R
Sbjct: 243 RIQEDSC-KLNTNSSET----------------------------KVKSKFCYGEKEKER 302

Query: 331 FNRILKNYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFML 390
           F+RIL  YVG++NFHNFTTRTKA+DP A R I+SF A+T + ++GI+F+KCEV+G+SFML
Sbjct: 303 FSRILSCYVGSYNFHNFTTRTKADDPTANRQIISFTANTVINLDGIDFIKCEVLGKSFML 362

Query: 391 HQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDS 450
           HQIRKMMGLAVAIMRNCA ESLI+ A  KDVNI VP APEVGLYLDEC FTSYN+ ++DS
Sbjct: 363 HQIRKMMGLAVAIMRNCASESLIQSAFSKDVNITVPMAPEVGLYLDECFFTSYNRNFEDS 422

Query: 451 HEEVSMKAYEEVAQDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDL 497
           HEEVSM+AY+E A+ FK+K IYSHI +TE K G +ALWLHSLN+RNYPDL
Sbjct: 423 HEEVSMEAYKEEAEAFKLKHIYSHIGATERKYGNMALWLHSLNYRNYPDL 437

BLAST of ClCG05G015300 vs. TAIR10
Match: AT2G30320.1 (AT2G30320.1 Pseudouridine synthase family protein)

HSP 1 Score: 120.9 bits (302), Expect = 2.3e-27
Identity = 119/442 (26.92%), Postives = 181/442 (40.95%), Query Frame = 1

Query: 6   PTETTRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSGAPKKQRYKRRKIAIFFAYCG 65
           PT   R  P  L+        + SST+ +    D   N  A K + Y+++K+ I   Y G
Sbjct: 18  PTRLIRVTPA-LLRFSSSAAFQPSSTSLSPPSSD---NFLADKWESYRKKKVVIRIGYVG 77

Query: 66  VGYQGMQ---KNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQ 125
             Y+G+Q    +P  KTIE +LE A+Y AG + + + G   +  WARS+RTDKGV ++  
Sbjct: 78  TDYRGLQIQRDDPSIKTIEGELEVAIYKAGGIRDSNYGDLHKIGWARSSRTDKGVHSLAT 137

Query: 126 VVSGRFYI-------DPPGFV--DRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYL 185
            +S +  I       DP G V    ++ +L   IR+F        F+ ++ C  R+Y YL
Sbjct: 138 SISLKMEIPETAWKDDPQGTVLAKCISKHLPENIRVFSVLPSNRRFDPRRECTLRKYSYL 197

Query: 186 IPVFALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSI---TD 245
           +P          D   +K S  SD       + +E  ++ EG     N+  ++     ++
Sbjct: 198 LP---------VDVLGIKNSFTSDEIDYHITDFNEILKEFEGEYPFHNYTQRSRYRRKSE 257

Query: 246 SDISSNTGKAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTE 305
             I    G+                +  S    ++    EN    + +G E   K     
Sbjct: 258 QKIKQRNGRP-------------PREPKSIKASESEFREEN---HIEIGEEEEEKEVD-- 317

Query: 306 MSGLGNDSEIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRT-KA 365
               G   E VV+ +S N        + Y  +E  + +       G H    F  R+ K 
Sbjct: 318 ----GESDEHVVTPDSDNSQVYSRAKWLYEPDETDKIS-------GAHFRKVFRCRSGKL 377

Query: 366 EDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLI 425
           E+                   G  FV+  + G+SFMLHQIRKM+G AVA+ R   P  +I
Sbjct: 378 ENSL-----------------GFGFVEISIWGESFMLHQIRKMIGTAVAVKRELLPRDII 400

Query: 426 EKALQKDVNINVPTAPEVGLYL 432
             +L K   I +P AP   L L
Sbjct: 438 RLSLNKFTRIVLPLAPSEVLIL 400

BLAST of ClCG05G015300 vs. TAIR10
Match: AT1G20290.1 (AT1G20290.1 SWI-SNF-related chromatin binding protein)

HSP 1 Score: 68.6 bits (166), Expect = 1.3e-11
Identity = 34/53 (64.15%), Postives = 36/53 (67.92%), Query Frame = 1

Query: 446 SHEEVSMKAYEEVAQD-FKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLR 498
           S  EVS+   +E   D FK K +YSHI S E KDGAVA+WLHSLN RNYPDLR
Sbjct: 82  SWREVSVDDDQEQEHDQFKWKHVYSHIGSAEEKDGAVAIWLHSLNQRNYPDLR 134

BLAST of ClCG05G015300 vs. NCBI nr
Match: gi|659104351|ref|XP_008452911.1| (PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo])

HSP 1 Score: 978.4 bits (2528), Expect = 4.9e-282
Identity = 489/516 (94.77%), Postives = 500/516 (96.90%), Query Frame = 1

Query: 1   MEEEPPTETTRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSGAPKKQRYKRRKIAIF 60
           MEEEPPTET RSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNS APKKQRYKRRK+AIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSNTGKA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKT+IT+SDISSN   A
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240

Query: 241 IEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSGLGNDSEI 300
           IEVSDVQ N VLE + TTSDSNKQTSVLVEN+ TEL V SEG AKSE +EMSGLGNDSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300

Query: 301 VVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNG EKPSGSFKYGEEEKARFNRILK+YVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEEVA+DFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQ 517
           VALWLHSLNHRNYPDLRV+DEVA+IAKNTSV METQ
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQ 516

BLAST of ClCG05G015300 vs. NCBI nr
Match: gi|449455575|ref|XP_004145528.1| (PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis sativus])

HSP 1 Score: 969.9 bits (2506), Expect = 1.7e-279
Identity = 484/516 (93.80%), Postives = 498/516 (96.51%), Query Frame = 1

Query: 1   MEEEPPTETTRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSGAPKKQRYKRRKIAIF 60
           MEEEPPTET RSPPQPLIEEP PKKLKMSSTTTTSDDEDVNSNS APKKQRYKRRK+AIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSNTGKA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGL+GKRNFEVKT+IT+SDISSNT  A
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNA 240

Query: 241 IEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSGLGNDSEI 300
           IEVSDVQ N VLE + TTSDSNKQTSVLVEN+ TELNVGSEGTAKSE +EMSGLG+DSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEI 300

Query: 301 VVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNG EK SGSFKYGEEEKARFNRILK+YVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDAS  VVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEE A+DFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQ 517
           VALWLHSLNHRNYPDLRV+DEVA+IAKN S+ METQ
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQ 516

BLAST of ClCG05G015300 vs. NCBI nr
Match: gi|1012001706|ref|XP_015932762.1| (PREDICTED: tRNA pseudouridine synthase A, mitochondrial isoform X1 [Arachis duranensis])

HSP 1 Score: 705.3 bits (1819), Expect = 7.9e-200
Identity = 364/525 (69.33%), Postives = 419/525 (79.81%), Query Frame = 1

Query: 7   TETTRSPPQP-----LIEEPDPKKLKMSSTTTTSDDEDVNSNSGAPKKQRYKRRKIAIFF 66
           T ++  PP P     + EEP+PKK KMS  T+TSDDE+  + +G   K+RYKRRKIAIFF
Sbjct: 9   TSSSLPPPPPPPALVVTEEPEPKKQKMS--TSTSDDEECITATGT--KKRYKRRKIAIFF 68

Query: 67  AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVG 126
           AYCGVGYQGMQKNPGAKTIE D+EEALY++GAVPEQDRG++KRYDWARSARTDKGVSAVG
Sbjct: 69  AYCGVGYQGMQKNPGAKTIEGDMEEALYVSGAVPEQDRGIAKRYDWARSARTDKGVSAVG 128

Query: 127 QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDP 186
           QVVSGRFYIDPPG VDRLNSNL  QIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDP
Sbjct: 129 QVVSGRFYIDPPGLVDRLNSNLPDQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDP 188

Query: 187 NCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMG----------------KRNFEVK 246
           +CHRDRE+V ASLGS NELVKCLECSERGRKV GL+G                +++ +V 
Sbjct: 189 SCHRDRETVLASLGSGNELVKCLECSERGRKVVGLVGNAKHNLTQEAMDVDSFRKDDKVN 248

Query: 247 TSIT---DSDISSNTGKAIEVSDVQENRVLEGN--DTTSDSNKQTSVLVENSCTELNVGS 306
             +T   D  +S   GK +      E++V+  N    T +S+ +  VLV+N  ++ ++ +
Sbjct: 249 AGLTEDIDVSLSKGEGKCLNEESGHEDKVVVDNLNSKTEESSNEVKVLVDNVNSKTDLET 308

Query: 307 EGTAKSEGTEMSGLGNDSEIVVSKESVNGNEKPS--GSFKYGEEEKARFNRILKNYVGTH 366
               +   T ++G G+ +  +V +E VNG ++P+    F YGEEEK RFNRILK YVGTH
Sbjct: 309 MVPVQDADTPVNG-GSVNTAIVEEEKVNGEDRPTKGSGFNYGEEEKERFNRILKYYVGTH 368

Query: 367 NFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVA 426
           NFHNFTTRTKAEDPAARRFI+SF+A TTVVVEG+EFVKCE+VGQSFMLHQIRKM+GLAVA
Sbjct: 369 NFHNFTTRTKAEDPAARRFIISFEAKTTVVVEGMEFVKCEIVGQSFMLHQIRKMVGLAVA 428

Query: 427 IMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEV 486
           IMRNCAPESLI KALQKDVNINVPTAPEVGLYLDEC F SYNQKWKDSHEE+SMKAYE+ 
Sbjct: 429 IMRNCAPESLINKALQKDVNINVPTAPEVGLYLDECFFASYNQKWKDSHEELSMKAYEKE 488

Query: 487 AQDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLRVVDEVA 504
           A+DFKMK IYSHIASTE KDG VALWLHSLNHRNYPDLR+VDE A
Sbjct: 489 AEDFKMKYIYSHIASTEQKDGTVALWLHSLNHRNYPDLRLVDEEA 528

BLAST of ClCG05G015300 vs. NCBI nr
Match: gi|1021473597|ref|XP_016200105.1| (PREDICTED: tRNA pseudouridine synthase A, mitochondrial isoform X1 [Arachis ipaensis])

HSP 1 Score: 697.6 bits (1799), Expect = 1.7e-197
Identity = 359/523 (68.64%), Postives = 416/523 (79.54%), Query Frame = 1

Query: 5   PPTETTRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSGAPKKQRYKRRKIAIFFAYC 64
           PP      P   + EEP+PKK KMS  T+TSDDE+  + +    K+RYKRRKIAIFFAYC
Sbjct: 14  PPPPPPPPPALVVTEEPEPKKQKMS--TSTSDDEECTTATDT--KKRYKRRKIAIFFAYC 73

Query: 65  GVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVV 124
           GVGYQGMQKNPGAKTIE D+EEALY++GAVPEQDRG++KRYDWARSARTDKGVSAVGQVV
Sbjct: 74  GVGYQGMQKNPGAKTIEGDMEEALYVSGAVPEQDRGIAKRYDWARSARTDKGVSAVGQVV 133

Query: 125 SGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCH 184
           SGRFYIDPPG VDR+NSNL  QIRIFGYKRVTASFNAK+FCDRRRYVYLIPVFALDP+CH
Sbjct: 134 SGRFYIDPPGLVDRVNSNLPDQIRIFGYKRVTASFNAKRFCDRRRYVYLIPVFALDPSCH 193

Query: 185 RDRESVKASLGSDNELVKCLECSERGRKVEGLMG----------------KRNFEVKTSI 244
           RDRE+V ASLG+ NELVKCLECSERGRKV GL+G                +++ +V   +
Sbjct: 194 RDRETVLASLGTGNELVKCLECSERGRKVVGLVGNAKHNLTQEAMDVDSFRKDDKVNAGL 253

Query: 245 T-DSDIS---SNTGKAIEVSDVQENRVLEGN--DTTSDSNKQTSVLVENSCTELNVGSEG 304
           T D D+S      GK +      E++V+  N    T +S+ +  VLV+N  ++ ++ +  
Sbjct: 254 TEDIDVSLSKGEEGKCLNEESGHEDKVVVDNLNSKTEESSNEVKVLVDNVNSKTDLETMV 313

Query: 305 TAKSEGTEMSGLGNDSEIVVSKESVNGNEKPS--GSFKYGEEEKARFNRILKNYVGTHNF 364
             +   T ++G G+ +  +V +E VNG ++P+    F YGEEEK RFNRILK YVGTHNF
Sbjct: 314 PVQDADTPVNG-GSVNTAIVEEEKVNGEDRPTKGSGFSYGEEEKERFNRILKYYVGTHNF 373

Query: 365 HNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIM 424
           HNFTTRTKAEDPAARRFI+SF+A TTVVVEG+EFVKCE+VGQSFMLHQIRKM+GLAVAIM
Sbjct: 374 HNFTTRTKAEDPAARRFIISFEAKTTVVVEGMEFVKCEIVGQSFMLHQIRKMVGLAVAIM 433

Query: 425 RNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQ 484
           RNCAPESLI KALQKDVNINVPTAPEVGLYLDEC F SYNQKWKDSHEE+SMKAYE+ A+
Sbjct: 434 RNCAPESLINKALQKDVNINVPTAPEVGLYLDECFFASYNQKWKDSHEELSMKAYEKEAE 493

Query: 485 DFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLRVVDEVA 504
           DFKMK IYSHIASTE +DG VALWLHSLNHRNYPDLR+VDE A
Sbjct: 494 DFKMKYIYSHIASTEQRDGTVALWLHSLNHRNYPDLRLVDEEA 531

BLAST of ClCG05G015300 vs. NCBI nr
Match: gi|645228848|ref|XP_008221186.1| (PREDICTED: tRNA pseudouridine synthase A, mitochondrial [Prunus mume])

HSP 1 Score: 696.8 bits (1797), Expect = 2.8e-197
Identity = 356/501 (71.06%), Postives = 403/501 (80.44%), Query Frame = 1

Query: 3   EEPPTETTRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSGAPKKQRYKRRKIAIFFA 62
           E P T+TT SPP    +EPDPKKLKMS+TTT+ DDE    ++   KKQRYKRRK+AIFFA
Sbjct: 2   ENPDTKTTISPPP---QEPDPKKLKMSTTTTSDDDETTTMDT--TKKQRYKRRKVAIFFA 61

Query: 63  YCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQ 122
           YCGVGYQGMQKNPGAKTIE DLEEALYL+ AVPEQDR   KRYDWARSARTDKGVSAVGQ
Sbjct: 62  YCGVGYQGMQKNPGAKTIEGDLEEALYLSNAVPEQDRNNPKRYDWARSARTDKGVSAVGQ 121

Query: 123 VVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPN 182
           VVSGRFY+DPPGFVDRLNSN+SPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDP+
Sbjct: 122 VVSGRFYVDPPGFVDRLNSNISPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPS 181

Query: 183 CHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSNTGKAIE 242
            HRDRESV ASLGSD E VKCLECSERGRKV GLMGKRN+E++ +    DISSNT +++ 
Sbjct: 182 AHRDRESVLASLGSDKEFVKCLECSERGRKVGGLMGKRNYELRGTSFGLDISSNTNESMV 241

Query: 243 VSDV-QENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSG--LGNDSE 302
            S+  +E +V   N    DS  +    +  S     V       SE T+++   L + ++
Sbjct: 242 ESEFNEETKVSPDNAGGDDSKSEPKNEISTSSNNNGVSGVEVEDSEDTDLNSEPLNDTTK 301

Query: 303 IVVSKESVNGNEKP---SGS-FKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAAR 362
           + V +E+ NG EKP   SGS F YGE+EK RFNRIL  Y GTHNFHNFTTRTKA DPAA 
Sbjct: 302 VSVGEENANGEEKPEKGSGSEFCYGEKEKDRFNRILNCYEGTHNFHNFTTRTKAADPAAW 361

Query: 363 RFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQK 422
           R+I+SF+A+TTV V G++FVKCEVVGQSFMLHQIRKM+GLAVAI RNCAP+SL+E ALQK
Sbjct: 362 RYIISFNANTTVTVGGMDFVKCEVVGQSFMLHQIRKMIGLAVAIFRNCAPDSLLEIALQK 421

Query: 423 DVNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSHIASTE 482
           DVN+NVPTAPEVGLYLDEC F SYNQKW DSHEE+SMK YE+ A+DFKMK IY HIASTE
Sbjct: 422 DVNVNVPTAPEVGLYLDECFFASYNQKWGDSHEELSMKDYEQEAEDFKMKHIYPHIASTE 481

Query: 483 HKDGAVALWLHSLNHRNYPDL 497
            K+G V LWLHSLNHRNYPDL
Sbjct: 482 QKEGVVGLWLHSLNHRNYPDL 497

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PUS1_SCHPO3.4e-4930.32tRNA pseudouridine synthase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
PUS1_CANAL8.3e-4030.77tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876... [more]
TRUA_MOUSE2.4e-3141.11tRNA pseudouridine synthase A, mitochondrial OS=Mus musculus GN=Pus1 PE=1 SV=2[more]
TRUA_RAT2.4e-3141.11tRNA pseudouridine synthase A, mitochondrial OS=Rattus norvegicus GN=Pus1 PE=1 S... [more]
TRUA_HUMAN6.0e-3040.00tRNA pseudouridine synthase A, mitochondrial OS=Homo sapiens GN=PUS1 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
E5GCD2_CUCME3.4e-28294.77tRNA pseudouridine synthase family protein OS=Cucumis melo subsp. melo PE=4 SV=1[more]
A0A0A0L0J9_CUCSA1.2e-27993.80Uncharacterized protein OS=Cucumis sativus GN=Csa_4G652870 PE=4 SV=1[more]
A0A0L9TQX3_PHAAN1.3e-19669.40Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g245700 PE=4 SV=1[more]
A0A0S3R6B4_PHAAN1.3e-19669.40Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G417100 PE=... [more]
A0A0B2S802_GLYSO2.4e-19569.88tRNA pseudouridine synthase 1 OS=Glycine soja GN=glysoja_036980 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G20370.13.6e-15860.74 Pseudouridine synthase family protein[more]
AT1G76120.12.9e-15260.64 Pseudouridine synthase family protein[more]
AT2G30320.12.3e-2726.92 Pseudouridine synthase family protein[more]
AT1G20290.11.3e-1164.15 SWI-SNF-related chromatin binding protein[more]
Match NameE-valueIdentityDescription
gi|659104351|ref|XP_008452911.1|4.9e-28294.77PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo][more]
gi|449455575|ref|XP_004145528.1|1.7e-27993.80PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis sativus][more]
gi|1012001706|ref|XP_015932762.1|7.9e-20069.33PREDICTED: tRNA pseudouridine synthase A, mitochondrial isoform X1 [Arachis dura... [more]
gi|1021473597|ref|XP_016200105.1|1.7e-19768.64PREDICTED: tRNA pseudouridine synthase A, mitochondrial isoform X1 [Arachis ipae... [more]
gi|645228848|ref|XP_008221186.1|2.8e-19771.06PREDICTED: tRNA pseudouridine synthase A, mitochondrial [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001406PsdUridine_synth_TruA
IPR020094Pseudouridine synthase I, TruA, N-terminal
IPR020095PsdUridine_synth_TruA_C
IPR020097PsdUridine_synth_TruA_a/b_dom
IPR020103PsdUridine_synth_cat_dom_sf
Vocabulary: Biological Process
TermDefinition
GO:0001522pseudouridine synthesis
GO:0009451RNA modification
Vocabulary: Molecular Function
TermDefinition
GO:0003723RNA binding
GO:0009982pseudouridine synthase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
cellular_component GO:0005575 cellular_component
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG05G015300.1ClCG05G015300.1mRNA


Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001406Pseudouridine synthase I, TruAPANTHERPTHR11142PSEUDOURIDYLATE SYNTHASEcoord: 318..510
score: 1.2E-174coord: 7..195
score: 1.2E
IPR020094Pseudouridine synthase I, TruA, N-terminalGENE3DG3DSA:3.30.70.580coord: 53..165
score: 1.2
IPR020095Pseudouridine synthase I, TruA, C-terminalGENE3DG3DSA:3.30.70.660coord: 318..437
score: 1.6
IPR020097Pseudouridine synthase I, TruA, alpha/beta domainPFAMPF01416PseudoU_synth_1coord: 331..436
score: 6.5
IPR020103Pseudouridine synthase, catalytic domainunknownSSF55120Pseudouridine synthasecoord: 53..189
score: 6.62E-62coord: 317..446
score: 6.62
NoneNo IPR availablePANTHERPTHR11142:SF4TRNA PSEUDOURIDINE SYNTHASE A, MITOCHONDRIALcoord: 318..510
score: 1.2E-174coord: 7..195
score: 1.2E