Cla97C05G096390 (gene) Watermelon (97103) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla97C05G096390
Type	gene
Organism	Citrullus lanatus (Watermelon (97103) v2)
Description	tRNA pseudouridine synthase family protein
Location	Cla97Chr05 : 25111523 .. 25113170 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGAAGAACCGCCAACTGAAACTACAAGGTCACCGCCACAACCGTTGATCGAAGAACCCGACCCAAAGAAGCTTAAAATGTCATCCACAACCACCACCTCCGACGACGAAGATGTCAATTCCAATTCTGGGGCGCCAAAGAAACAAAGATACAAGCGCCGCAAGATCGCCATTTTCTTTGCCTACTGTGGGGTAGGTTACCAAGGGATGCAGAAGAATCCGGGCGCTAAAACCATTGAAGCTGACCTTGAAGAAGCCCTATATCTCGCCGGAGCAGTCCCGGAGCAGGACCGAGGGCTCTCCAAGCGCTACGATTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTGAGTGCCGTGGGTCAGGTTGTTTCCGGTCGCTTCTACATCGATCCGCCAGGTTTTGTTGATCGTCTCAATTCAAATCTTTCGCCTCAGATTCGAATTTTCGGGTACAAGCGCGTCACAGCGTCATTCAATGCGAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATTCCTGTATTCGCTCTCGATCCCAATTGTCATCGTGATAGAGAAAGTGTGAAAGCTAGTTTAGGATCTGACAACGAGCTTGTGAAGTGCTTAGAGTGCTCTGAACGAGGACGCAAGGTCGAAGGCTTGATGGGCAAGCGCAATTTTGAAGTAAAAACTTCAATAACTGACTCAGACATTTCTTCGAACACTGGAAAAGCCATTGAAGTTTCTGATGTTCAGGAAAATAGGGTTTTGGAGGGCAATGACACGACCTCTGATTCTAACAAACAAACTAGCGTTTTGGTTGAAAATAGTTGTACTGAACTCAATGTTGGAAGCGAAGGTACTGCAAAAAGTGAAGGCACTGAAATGTCTGGATTAGGGAATGATTCAGAGATTGTTGTAAGCAAAGAGAGCGTTAATGGGAACGAAAAACCCTCAGGGAGCTTCAAGTATGGCGAGGAGGAGAAGGCTAGATTCAATAGGATTTTGAAGAATTATGTGGGAACTCATAACTTCCATAATTTTACAACCAGAACAAAAGCAGAGGACCCTGCTGCTCGTCGGTTCATTGTTTCATTTGATGCGTCTACCACTGTCGTGGTTGAAGGCATCGAATTTGTGAAATGTGAAGTTGTGGGGCAGAGCTTCATGCTTCATCAGATCCGAAAGATGATGGGTCTTGCTGTGGCAATCATGAGGAACTGTGCTCCAGAGTCATTGATTGAGAAGGCTTTGCAAAAGTAAGTAATTTCCATTTTCAACTACAATTTGATTTGATTTTCATTTGTTTAATAGAATTGAATTGGATTCAAGTAATCATAATTCAAACTACAGGGATGTAAATATCAATGTGCCCACTGCACCTGAGGTTGGGCTGTACTTAGATGAGTGCCTATTCACATCGTATAATCAAAAGTGGAAAGACAGTCATGAAGAGGTATCTATGAAAGCATACGAAGAGGTAGCACAAGATTTCAAAATGAAGCAAATATATTCACACATAGCTTCCACAGAGCACAAAGATGGAGCCGTGGCCTTATGGTTGCATTCTCTAAACCATCGGAATTATCCAGATTTACGGGTTGTTGATGAGGTTGCAGAAATTGCTAAGAACACAAGTGTTGCCATGGAGACACAAGGCTGA

mRNA sequence

ATGGAAGAAGAACCGCCAACTGAAACTACAAGGTCACCGCCACAACCGTTGATCGAAGAACCCGACCCAAAGAAGCTTAAAATGTCATCCACAACCACCACCTCCGACGACGAAGATGTCAATTCCAATTCTGGGGCGCCAAAGAAACAAAGATACAAGCGCCGCAAGATCGCCATTTTCTTTGCCTACTGTGGGGTAGGTTACCAAGGGATGCAGAAGAATCCGGGCGCTAAAACCATTGAAGCTGACCTTGAAGAAGCCCTATATCTCGCCGGAGCAGTCCCGGAGCAGGACCGAGGGCTCTCCAAGCGCTACGATTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTGAGTGCCGTGGGTCAGGTTGTTTCCGGTCGCTTCTACATCGATCCGCCAGGTTTTGTTGATCGTCTCAATTCAAATCTTTCGCCTCAGATTCGAATTTTCGGGTACAAGCGCGTCACAGCGTCATTCAATGCGAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATTCCTGTATTCGCTCTCGATCCCAATTGTCATCGTGATAGAGAAAGTGTGAAAGCTAGTTTAGGATCTGACAACGAGCTTGTGAAGTGCTTAGAGTGCTCTGAACGAGGACGCAAGGTCGAAGGCTTGATGGGCAAGCGCAATTTTGAAGTAAAAACTTCAATAACTGACTCAGACATTTCTTCGAACACTGGAAAAGCCATTGAAGTTTCTGATGTTCAGGAAAATAGGGTTTTGGAGGGCAATGACACGACCTCTGATTCTAACAAACAAACTAGCGTTTTGGTTGAAAATAGTTGTACTGAACTCAATGTTGGAAGCGAAGGTACTGCAAAAAGTGAAGGCACTGAAATGTCTGGATTAGGGAATGATTCAGAGATTGTTGTAAGCAAAGAGAGCGTTAATGGGAACGAAAAACCCTCAGGGAGCTTCAAGTATGGCGAGGAGGAGAAGGCTAGATTCAATAGGATTTTGAAGAATTATGTGGGAACTCATAACTTCCATAATTTTACAACCAGAACAAAAGCAGAGGACCCTGCTGCTCGTCGGTTCATTGTTTCATTTGATGCGTCTACCACTGTCGTGGTTGAAGGCATCGAATTTGTGAAATGTGAAGTTGTGGGGCAGAGCTTCATGCTTCATCAGATCCGAAAGATGATGGGTCTTGCTGTGGCAATCATGAGGAACTGTGCTCCAGAGTCATTGATTGAGAAGGCTTTGCAAAAGGATGTAAATATCAATGTGCCCACTGCACCTGAGGTTGGGCTGTACTTAGATGAGTGCCTATTCACATCGTATAATCAAAAGTGGAAAGACAGTCATGAAGAGGTATCTATGAAAGCATACGAAGAGGTAGCACAAGATTTCAAAATGAAGCAAATATATTCACACATAGCTTCCACAGAGCACAAAGATGGAGCCGTGGCCTTATGGTTGCATTCTCTAAACCATCGGAATTATCCAGATTTACGGGTTGTTGATGAGGTTGCAGAAATTGCTAAGAACACAAGTGTTGCCATGGAGACACAAGGCTGA

Coding sequence (CDS)

ATGGAAGAAGAACCGCCAACTGAAACTACAAGGTCACCGCCACAACCGTTGATCGAAGAACCCGACCCAAAGAAGCTTAAAATGTCATCCACAACCACCACCTCCGACGACGAAGATGTCAATTCCAATTCTGGGGCGCCAAAGAAACAAAGATACAAGCGCCGCAAGATCGCCATTTTCTTTGCCTACTGTGGGGTAGGTTACCAAGGGATGCAGAAGAATCCGGGCGCTAAAACCATTGAAGCTGACCTTGAAGAAGCCCTATATCTCGCCGGAGCAGTCCCGGAGCAGGACCGAGGGCTCTCCAAGCGCTACGATTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTGAGTGCCGTGGGTCAGGTTGTTTCCGGTCGCTTCTACATCGATCCGCCAGGTTTTGTTGATCGTCTCAATTCAAATCTTTCGCCTCAGATTCGAATTTTCGGGTACAAGCGCGTCACAGCGTCATTCAATGCGAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATTCCTGTATTCGCTCTCGATCCCAATTGTCATCGTGATAGAGAAAGTGTGAAAGCTAGTTTAGGATCTGACAACGAGCTTGTGAAGTGCTTAGAGTGCTCTGAACGAGGACGCAAGGTCGAAGGCTTGATGGGCAAGCGCAATTTTGAAGTAAAAACTTCAATAACTGACTCAGACATTTCTTCGAACACTGGAAAAGCCATTGAAGTTTCTGATGTTCAGGAAAATAGGGTTTTGGAGGGCAATGACACGACCTCTGATTCTAACAAACAAACTAGCGTTTTGGTTGAAAATAGTTGTACTGAACTCAATGTTGGAAGCGAAGGTACTGCAAAAAGTGAAGGCACTGAAATGTCTGGATTAGGGAATGATTCAGAGATTGTTGTAAGCAAAGAGAGCGTTAATGGGAACGAAAAACCCTCAGGGAGCTTCAAGTATGGCGAGGAGGAGAAGGCTAGATTCAATAGGATTTTGAAGAATTATGTGGGAACTCATAACTTCCATAATTTTACAACCAGAACAAAAGCAGAGGACCCTGCTGCTCGTCGGTTCATTGTTTCATTTGATGCGTCTACCACTGTCGTGGTTGAAGGCATCGAATTTGTGAAATGTGAAGTTGTGGGGCAGAGCTTCATGCTTCATCAGATCCGAAAGATGATGGGTCTTGCTGTGGCAATCATGAGGAACTGTGCTCCAGAGTCATTGATTGAGAAGGCTTTGCAAAAGGATGTAAATATCAATGTGCCCACTGCACCTGAGGTTGGGCTGTACTTAGATGAGTGCCTATTCACATCGTATAATCAAAAGTGGAAAGACAGTCATGAAGAGGTATCTATGAAAGCATACGAAGAGGTAGCACAAGATTTCAAAATGAAGCAAATATATTCACACATAGCTTCCACAGAGCACAAAGATGGAGCCGTGGCCTTATGGTTGCATTCTCTAAACCATCGGAATTATCCAGATTTACGGGTTGTTGATGAGGTTGCAGAAATTGCTAAGAACACAAGTGTTGCCATGGAGACACAAGGCTGA

Protein sequence

MEEEPPTETTRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSGAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSNTGKAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSGLGNDSEIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQG

Homology

[Close]

BLAST of Cla97C05G096390 vs. NCBI nr
Match: XP_008452911.1 (PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo] >XP_008452912.1 PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo] >ADN34131.1 tRNA pseudouridine synthase family protein [Cucumis melo subsp. melo])

HSP 1 Score: 858.6 bits (2217), Expect = 1.1e-245
Identity = 434/458 (94.76%), Postives = 444/458 (96.94%), Query Frame = 0


Query: 59  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 118
           IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS
Sbjct: 59  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 118

Query: 119 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 178
           AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA
Sbjct: 119 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 178

Query: 179 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSNTG 238
           LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKT+IT+SDISSN  
Sbjct: 179 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIV 238

Query: 239 KAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSGLGNDS 298
            AIEVSDVQ N VLE + TTSDSNKQTSVLVEN+ TEL V SEG AKSE +EMSGLGNDS
Sbjct: 239 NAIEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDS 298

Query: 299 EIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFI 358
           EIVVSKESVNG EKPSGSFKYGEEEKARFNRILK+YVGTHNFHNFTTRTKAEDPAARRFI
Sbjct: 299 EIVVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFI 358

Query: 359 VSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVN 418
           VSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVN
Sbjct: 359 VSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVN 418

Query: 419 INVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSHIASTEHKD 478
           INVPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEEVA+DFKMKQIYSHIASTEHKD
Sbjct: 419 INVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKD 478

Query: 479 GAVALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQ 517
           GAVALWLHSLNHRNYPDLRV+DEVA+IAKNTSV METQ
Sbjct: 479 GAVALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQ 516

[Close]

BLAST of Cla97C05G096390 vs. NCBI nr
Match: XP_004145528.1 (PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis sativus] >KGN55475.1 hypothetical protein Csa_4G652870 [Cucumis sativus])

HSP 1 Score: 852.4 bits (2201), Expect = 7.8e-244
Identity = 430/458 (93.89%), Postives = 443/458 (96.72%), Query Frame = 0


Query: 59  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 118
           IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS
Sbjct: 59  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 118

Query: 119 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 178
           AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA
Sbjct: 119 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 178

Query: 179 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSNTG 238
           LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGL+GKRNFEVKT+IT+SDISSNT 
Sbjct: 179 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTV 238

Query: 239 KAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSGLGNDS 298
            AIEVSDVQ N VLE + TTSDSNKQTSVLVEN+ TELNVGSEGTAKSE +EMSGLG+DS
Sbjct: 239 NAIEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDS 298

Query: 299 EIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFI 358
           EIVVSKESVNG EK SGSFKYGEEEKARFNRILK+YVGTHNFHNFTTRTKAEDPAARRFI
Sbjct: 299 EIVVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFI 358

Query: 359 VSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVN 418
           VSFDAS  VVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVN
Sbjct: 359 VSFDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVN 418

Query: 419 INVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSHIASTEHKD 478
           INVPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEE A+DFKMKQIYSHIASTEHKD
Sbjct: 419 INVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKD 478

Query: 479 GAVALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQ 517
           GAVALWLHSLNHRNYPDLRV+DEVA+IAKN S+ METQ
Sbjct: 479 GAVALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQ 516

[Close]

BLAST of Cla97C05G096390 vs. NCBI nr
Match: XP_022975443.1 (tRNA pseudouridine synthase A [Cucurbita maxima])

HSP 1 Score: 836.3 bits (2159), Expect = 5.8e-239
Identity = 422/460 (91.74%), Postives = 436/460 (94.78%), Query Frame = 0


Query: 57  IAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKG 116
           IAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKG
Sbjct: 57  IAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKG 116

Query: 117 VSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPV 176
           VSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPV
Sbjct: 117 VSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPV 176

Query: 177 FALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSN 236
           FALDPNCHRDRESVKASLGS NELVKCLECSERGRKVEGLMGKRNFEVKT+I++SDIS N
Sbjct: 177 FALDPNCHRDRESVKASLGSGNELVKCLECSERGRKVEGLMGKRNFEVKTAISESDISPN 236

Query: 237 TGKAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSGLGN 296
            G AIEVSDVQ N  LE N T SDS K+TSVLVENS TELNVGSE  AKSEG+EMSGLGN
Sbjct: 237 FGNAIEVSDVQANGALEDNATASDSIKETSVLVENSGTELNVGSEAIAKSEGSEMSGLGN 296

Query: 297 DSEIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARR 356
           DSE VVSK+SVN NEKPSGSFKYGEEEKARFNRILK YVGTHNFHNFTTRTKAEDPAARR
Sbjct: 297 DSENVVSKDSVNRNEKPSGSFKYGEEEKARFNRILKKYVGTHNFHNFTTRTKAEDPAARR 356

Query: 357 FIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKD 416
           FI+SFDA+ TVVVEG+EFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQ+D
Sbjct: 357 FIISFDAAITVVVEGVEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQQD 416

Query: 417 VNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSHIASTEH 476
           VNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVA+DFKMK IYSHI STEH
Sbjct: 417 VNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKHIYSHIGSTEH 476

Query: 477 KDGAVALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQ 517
           K+GAVALWLHSLNHRNYPDLRV DE A+ AKNTSV +ETQ
Sbjct: 477 KEGAVALWLHSLNHRNYPDLRVADEAADNAKNTSVDIETQ 516

[Close]

BLAST of Cla97C05G096390 vs. NCBI nr
Match: XP_022936288.1 (tRNA pseudouridine synthase A [Cucurbita moschata])

HSP 1 Score: 828.2 bits (2138), Expect = 1.6e-236
Identity = 420/461 (91.11%), Postives = 434/461 (94.14%), Query Frame = 0


Query: 57  IAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKG 116
           IAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKG
Sbjct: 57  IAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKG 116

Query: 117 VSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPV 176
           VSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPV
Sbjct: 117 VSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPV 176

Query: 177 FALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSN 236
           FALDPNCHRDRESVKASLGS NELVKCLECSERGRKVEGLMGKRNFEVKT+I++SDIS N
Sbjct: 177 FALDPNCHRDRESVKASLGSGNELVKCLECSERGRKVEGLMGKRNFEVKTAISESDISPN 236

Query: 237 TGKAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSGLGN 296
           +G AIEV DVQ N  LE N T SDS K+TSVLVENS TELNVG E TAK EG+EMSGLGN
Sbjct: 237 SGNAIEVPDVQANGALEDNATASDSIKETSVLVENSGTELNVGGEATAKIEGSEMSGLGN 296

Query: 297 DSEIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARR 356
           DSE V SKESVN NEK SGSFKYGEEEKARFNRILK YVGTHNFHNFTTRTKAEDPAARR
Sbjct: 297 DSENVASKESVNRNEKSSGSFKYGEEEKARFNRILKKYVGTHNFHNFTTRTKAEDPAARR 356

Query: 357 FIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKD 416
           FI+SFDA+TTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQ+D
Sbjct: 357 FIISFDAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQQD 416

Query: 417 VNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSHIASTEH 476
           VNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVA+DFKMK IYSHI STEH
Sbjct: 417 VNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKHIYSHIGSTEH 476

Query: 477 KDGAVALWLHSLNHRNYPDLRVVDEVA-EIAKNTSVAMETQ 517
           K+GAVALWLHSLNHRNYPDLRV DE A + AKNTSV ++TQ
Sbjct: 477 KEGAVALWLHSLNHRNYPDLRVADEAADDNAKNTSVGIDTQ 517

[Close]

BLAST of Cla97C05G096390 vs. NCBI nr
Match: XP_023536425.1 (tRNA pseudouridine synthase A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 819.3 bits (2115), Expect = 7.3e-234
Identity = 416/460 (90.43%), Postives = 431/460 (93.70%), Query Frame = 0


Query: 57  IAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKG 116
           IAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYL+GAVPEQDRGLSKRYDWARSARTDKG
Sbjct: 57  IAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLSGAVPEQDRGLSKRYDWARSARTDKG 116

Query: 117 VSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPV 176
           VSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPV
Sbjct: 117 VSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPV 176

Query: 177 FALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSN 236
           FALDPNCHRDRESVKASLGS NELVKCLECSERGRKVEGLMGKRNFEVKT+I++SDIS N
Sbjct: 177 FALDPNCHRDRESVKASLGSGNELVKCLECSERGRKVEGLMGKRNFEVKTAISESDISPN 236

Query: 237 TGKAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSGLGN 296
           +G AIEVSDVQ N  LE N T SDS K+     ENS TELNVGSE TAK EG EMSGLGN
Sbjct: 237 SGNAIEVSDVQANGALEDNATASDSIKE-----ENSGTELNVGSEATAKIEGCEMSGLGN 296

Query: 297 DSEIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARR 356
           DSE VVSKESVN NEK SGSFKYGEEEKARFNRILK YVGTHNFHNFTTRTKAEDPAARR
Sbjct: 297 DSENVVSKESVNRNEKSSGSFKYGEEEKARFNRILKKYVGTHNFHNFTTRTKAEDPAARR 356

Query: 357 FIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKD 416
           FI+SFDA+TTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQ+D
Sbjct: 357 FIISFDAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQQD 416

Query: 417 VNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSHIASTEH 476
           VNIN+PTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVA+DFKMK IYSHI STEH
Sbjct: 417 VNINLPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKHIYSHIGSTEH 476

Query: 477 KDGAVALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQ 517
           K+GAVALWLHSLNHRNYPDLRV DE A+ AKNTSV ++TQ
Sbjct: 477 KEGAVALWLHSLNHRNYPDLRVADEAADNAKNTSVDIDTQ 511

[Close]

BLAST of Cla97C05G096390 vs. TrEMBL
Match: tr|A0A1S3BV07|A0A1S3BV07_CUCME (tRNA pseudouridine synthase A, mitochondrial-like OS=Cucumis melo OX=3656 GN=LOC103493789 PE=4 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 7.2e-246
Identity = 434/458 (94.76%), Postives = 444/458 (96.94%), Query Frame = 0


Query: 59  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 118
           IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS
Sbjct: 59  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 118

Query: 119 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 178
           AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA
Sbjct: 119 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 178

Query: 179 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSNTG 238
           LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKT+IT+SDISSN  
Sbjct: 179 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIV 238

Query: 239 KAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSGLGNDS 298
            AIEVSDVQ N VLE + TTSDSNKQTSVLVEN+ TEL V SEG AKSE +EMSGLGNDS
Sbjct: 239 NAIEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDS 298

Query: 299 EIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFI 358
           EIVVSKESVNG EKPSGSFKYGEEEKARFNRILK+YVGTHNFHNFTTRTKAEDPAARRFI
Sbjct: 299 EIVVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFI 358

Query: 359 VSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVN 418
           VSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVN
Sbjct: 359 VSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVN 418

Query: 419 INVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSHIASTEHKD 478
           INVPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEEVA+DFKMKQIYSHIASTEHKD
Sbjct: 419 INVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKD 478

Query: 479 GAVALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQ 517
           GAVALWLHSLNHRNYPDLRV+DEVA+IAKNTSV METQ
Sbjct: 479 GAVALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQ 516

[Close]

BLAST of Cla97C05G096390 vs. TrEMBL
Match: tr|E5GCD2|E5GCD2_CUCME (tRNA pseudouridine synthase family protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 7.2e-246
Identity = 434/458 (94.76%), Postives = 444/458 (96.94%), Query Frame = 0


Query: 59  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 118
           IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS
Sbjct: 59  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 118

Query: 119 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 178
           AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA
Sbjct: 119 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 178

Query: 179 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSNTG 238
           LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKT+IT+SDISSN  
Sbjct: 179 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIV 238

Query: 239 KAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSGLGNDS 298
            AIEVSDVQ N VLE + TTSDSNKQTSVLVEN+ TEL V SEG AKSE +EMSGLGNDS
Sbjct: 239 NAIEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDS 298

Query: 299 EIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFI 358
           EIVVSKESVNG EKPSGSFKYGEEEKARFNRILK+YVGTHNFHNFTTRTKAEDPAARRFI
Sbjct: 299 EIVVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFI 358

Query: 359 VSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVN 418
           VSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVN
Sbjct: 359 VSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVN 418

Query: 419 INVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSHIASTEHKD 478
           INVPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEEVA+DFKMKQIYSHIASTEHKD
Sbjct: 419 INVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKD 478

Query: 479 GAVALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQ 517
           GAVALWLHSLNHRNYPDLRV+DEVA+IAKNTSV METQ
Sbjct: 479 GAVALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQ 516

[Close]

BLAST of Cla97C05G096390 vs. TrEMBL
Match: tr|A0A0A0L0J9|A0A0A0L0J9_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G652870 PE=4 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 5.2e-244
Identity = 430/458 (93.89%), Postives = 443/458 (96.72%), Query Frame = 0


Query: 59  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 118
           IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS
Sbjct: 59  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 118

Query: 119 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 178
           AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA
Sbjct: 119 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 178

Query: 179 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSNTG 238
           LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGL+GKRNFEVKT+IT+SDISSNT 
Sbjct: 179 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTV 238

Query: 239 KAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSGLGNDS 298
            AIEVSDVQ N VLE + TTSDSNKQTSVLVEN+ TELNVGSEGTAKSE +EMSGLG+DS
Sbjct: 239 NAIEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDS 298

Query: 299 EIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFI 358
           EIVVSKESVNG EK SGSFKYGEEEKARFNRILK+YVGTHNFHNFTTRTKAEDPAARRFI
Sbjct: 299 EIVVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFI 358

Query: 359 VSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVN 418
           VSFDAS  VVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVN
Sbjct: 359 VSFDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVN 418

Query: 419 INVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSHIASTEHKD 478
           INVPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEE A+DFKMKQIYSHIASTEHKD
Sbjct: 419 INVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKD 478

Query: 479 GAVALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQ 517
           GAVALWLHSLNHRNYPDLRV+DEVA+IAKN S+ METQ
Sbjct: 479 GAVALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQ 516

[Close]

BLAST of Cla97C05G096390 vs. TrEMBL
Match: tr|A0A2N9FMA8|A0A2N9FMA8_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS15931 PE=4 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.2e-184
Identity = 338/450 (75.11%), Postives = 374/450 (83.11%), Query Frame = 0


Query: 57  IAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKG 116
           IAIFFAYCGVGYQGMQKNPGAKTIE+DLEEALYL+GAVPE DR   KRYDWARSARTDKG
Sbjct: 54  IAIFFAYCGVGYQGMQKNPGAKTIESDLEEALYLSGAVPEHDRNNPKRYDWARSARTDKG 113

Query: 117 VSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPV 176
           VSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVT SFNAKKFCDRRRYVYLIPV
Sbjct: 114 VSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTGSFNAKKFCDRRRYVYLIPV 173

Query: 177 FALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSN 236
           FALDP+ H DRE+V ASLGS NELVKCLECSERGRKV G+MG+RNFE +    +  ISSN
Sbjct: 174 FALDPSAHPDRETVLASLGSGNELVKCLECSERGRKVVGVMGRRNFEFRAVSPELGISSN 233

Query: 237 TGKAIEVSDVQENRV----LEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMS 296
           +G AI  S  +ENRV     + + + S+S ++  V+  N  +E+ + +  +A++EGT+  
Sbjct: 234 SGDAIVES--KENRVSFENADDDKSNSESVEEPKVVNHNGASEIALETVISAQNEGTD-- 293

Query: 297 GLGNDSEIVVSKESVNGNEK--PSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAE 356
                       E VNG EK      F YGE EK RFN ILK+YVGTHNFHNFTTRTKAE
Sbjct: 294 ------------EKVNGEEKGAKGSEFCYGEREKERFNAILKHYVGTHNFHNFTTRTKAE 353

Query: 357 DPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIE 416
           DPAA+RFI+SF+A T V VEGIEFV+CEVVGQSFMLHQIRKM+GLAVAIMRNCAPESLIE
Sbjct: 354 DPAAQRFIISFEAKTVVTVEGIEFVRCEVVGQSFMLHQIRKMIGLAVAIMRNCAPESLIE 413

Query: 417 KALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSH 476
            A QKDVNINVPTAPEVGLYL+EC FTSYNQKWKDSHEEVSMKAYEE A++FKMK IYSH
Sbjct: 414 TAFQKDVNINVPTAPEVGLYLEECFFTSYNQKWKDSHEEVSMKAYEEEAEEFKMKHIYSH 473

Query: 477 IASTEHKDGAVALWLHSLNHRNYPDLRVVD 501
           IASTEHK+G VALWLHSLNHRNYPDLRVV+
Sbjct: 474 IASTEHKEGVVALWLHSLNHRNYPDLRVVN 487

[Close]

BLAST of Cla97C05G096390 vs. TrEMBL
Match: tr|A0A2K3NXY5|A0A2K3NXY5_TRIPR (tRNA pseudouridine synthase A mitochondrial-like OS=Trifolium pratense OX=57577 GN=L195_g004408 PE=4 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 4.8e-181
Identity = 340/458 (74.24%), Postives = 380/458 (82.97%), Query Frame = 0


Query: 57  IAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKG 116
           +AIFFAYCGVGYQGMQKNPGAKTIE +LEEALY++GAVPEQDRGLSKRYDWARSARTDKG
Sbjct: 56  VAIFFAYCGVGYQGMQKNPGAKTIEGELEEALYVSGAVPEQDRGLSKRYDWARSARTDKG 115

Query: 117 VSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPV 176
           VSAVGQVVSGRFYIDPPGF+DRLNSNL  QIRIFG+KRVTASF+AKKFCDRRRYVYLIPV
Sbjct: 116 VSAVGQVVSGRFYIDPPGFIDRLNSNLPSQIRIFGFKRVTASFSAKKFCDRRRYVYLIPV 175

Query: 177 FALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMG--KRNFEVKTSITDSDIS 236
           FALDP CHRDRE+V ASLGS+NELVKCLECSERGRKVEGL G  KRN E +    ++D S
Sbjct: 176 FALDPCCHRDRETVLASLGSENELVKCLECSERGRKVEGLFGNSKRNLEQEAVDVETDGS 235

Query: 237 SNTGKAIEVSDVQEN---RVLEGNDTTSDSNKQTSVLVENSCTELN--VGSEGTAKSEGT 296
           SN   A+  S V EN    + +G+D+  +         + S  ELN   G E T  S+  
Sbjct: 236 SNR-TAVANSGVAENVEVSLSKGDDSRLNKESINDNEGKVSVEELNYKTGLE-TVVSDQD 295

Query: 297 EMSGLGNDSEI---VVSKESVNGNE---KPSGSFKYGEEEKARFNRILKNYVGTHNFHNF 356
           ++S L   SE    V+ +E VNG +   K SG F YGE+E+ RFN+ILK YVGTHNFHNF
Sbjct: 296 KVSSLNGGSENNSGVLKEEKVNGEDTAAKGSG-FSYGEKERERFNKILKYYVGTHNFHNF 355

Query: 357 TTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNC 416
           TTR K EDP+A RFI+SFDASTTVVVEG+EFVKCE+VGQSFMLHQIRKMMGLAVAIMRNC
Sbjct: 356 TTRIKPEDPSAHRFIISFDASTTVVVEGMEFVKCEIVGQSFMLHQIRKMMGLAVAIMRNC 415

Query: 417 APESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFK 476
           APESLIEKALQKDV+I VPTAPEVGLYLDEC FTSYNQKWKD+HEEVSMKAYE+ A+DFK
Sbjct: 416 APESLIEKALQKDVSITVPTAPEVGLYLDECFFTSYNQKWKDTHEEVSMKAYEKEAEDFK 475

Query: 477 MKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLRVVDE 502
           MK IYSHIASTE+K+G V LWLHSLNHRNYPDLR++DE
Sbjct: 476 MKYIYSHIASTEYKEGTVGLWLHSLNHRNYPDLRILDE 510

[Close]

BLAST of Cla97C05G096390 vs. Swiss-Prot
Match: sp|Q9WU56|TRUA_MOUSE (tRNA pseudouridine synthase A OS=Mus musculus OX=10090 GN=Pus1 PE=1 SV=2)

HSP 1 Score: 197.2 bits (500), Expect = 4.5e-49
Identity = 140/442 (31.67%), Postives = 196/442 (44.34%), Query Frame = 0


Query: 57  IAIFFAYCGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEQDRGLSKRYDWARSART 116
           I +  AY G GY GMQ+N G+   +TIE DL  AL  AG +PE      ++  + R ART
Sbjct: 82  IVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCART 141

Query: 117 DKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYL 176
           DKGVSA GQVVS + ++     +D++NS+L   IRI G KRVT  FN+K  CD R Y Y+
Sbjct: 142 DKGVSAAGQVVSLKVWL-IDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYM 201

Query: 177 IPVFALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDI 236
           +P FA     H+DR                                              
Sbjct: 202 LPTFAF---AHKDR---------------------------------------------- 261

Query: 237 SSNTGKAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSG 296
                      DVQ+                                             
Sbjct: 262 -----------DVQDE-------------------------------------------- 321

Query: 297 LGNDSEIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPA 356
                                 S++   E   + NR+L  Y GTHNFHNFT++    +P+
Sbjct: 322 ----------------------SYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPS 381

Query: 357 ARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKAL 416
           ARR+I+        V EG+EF   +V GQSFM+HQIRKM+GL VAI++  APES++E++ 
Sbjct: 382 ARRYILEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSW 395

Query: 417 QKDVNINVPTAPEVGLYLDECLFTSYNQKWKDS--HEEVSMKAYEEVAQDFKMKQIYSHI 476
            ++  ++VP AP +GL L+   F  YNQ++     HE +     E     FK + IY  I
Sbjct: 442 GEE-KVDVPKAPGLGLVLERVHFEKYNQRFGGDGLHEPLDWTQEEGKVTAFKEQYIYPTI 395

Query: 477 ASTEHKDGAVALWLHSLNHRNY 494
            STE  + ++A WL++L   N+
Sbjct: 502 VSTERDERSMAQWLNTLPIHNF 395

[Close]

BLAST of Cla97C05G096390 vs. Swiss-Prot
Match: sp|Q4KM92|TRUA_RAT (tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2)

HSP 1 Score: 197.2 bits (500), Expect = 4.5e-49
Identity = 140/442 (31.67%), Postives = 196/442 (44.34%), Query Frame = 0


Query: 57  IAIFFAYCGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEQDRGLSKRYDWARSART 116
           I +  AY G GY GMQ+N G+   +TIE DL  AL  AG +PE      ++  + R ART
Sbjct: 82  IVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCART 141

Query: 117 DKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYL 176
           DKGVSA GQVVS + ++     +D++NS+L   IRI G KRVT  FN+K  CD R Y Y+
Sbjct: 142 DKGVSAAGQVVSLKVWL-IDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYM 201

Query: 177 IPVFALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDI 236
           +P FA     H+DR                                              
Sbjct: 202 LPTFAF---AHKDR---------------------------------------------- 261

Query: 237 SSNTGKAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSG 296
                      DVQ+                                             
Sbjct: 262 -----------DVQDE-------------------------------------------- 321

Query: 297 LGNDSEIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPA 356
                                 S++   E   + NR+L  Y GTHNFHNFT++    +P+
Sbjct: 322 ----------------------SYRLSAETLQQVNRLLGCYKGTHNFHNFTSQKGPREPS 381

Query: 357 ARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKAL 416
           ARR+I+        V EG+EF   +V GQSFM+HQIRKM+GL VAI++  APES++E++ 
Sbjct: 382 ARRYILEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSW 395

Query: 417 QKDVNINVPTAPEVGLYLDECLFTSYNQKWKDS--HEEVSMKAYEEVAQDFKMKQIYSHI 476
            ++  ++VP AP +GL L+   F  YNQ++     HE +     E     FK + IY  I
Sbjct: 442 GEE-KVDVPKAPGLGLVLERVHFEKYNQRFGSDGLHEPLDWAQEEGKVTAFKEQYIYPTI 395

Query: 477 ASTEHKDGAVALWLHSLNHRNY 494
            STE  + ++A WL++L   N+
Sbjct: 502 VSTERDERSMAQWLNTLPIHNF 395

[Close]

BLAST of Cla97C05G096390 vs. Swiss-Prot
Match: sp|Q9Y606|TRUA_HUMAN (tRNA pseudouridine synthase A OS=Homo sapiens OX=9606 GN=PUS1 PE=1 SV=3)

HSP 1 Score: 189.9 bits (481), Expect = 7.1e-47
Identity = 137/437 (31.35%), Postives = 191/437 (43.71%), Query Frame = 0


Query: 62  AYCGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 121
           AY G GY GMQ+N G+   KTIE DL  AL  +G +PE      ++  + R ARTDKGVS
Sbjct: 91  AYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVS 150

Query: 122 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 181
           A GQVVS + ++     ++++NS+L   IRI G KRVT  FN+K  CD R Y YL+P FA
Sbjct: 151 AAGQVVSLKVWL-IDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFA 210

Query: 182 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDISSNTG 241
                H+DR                                                   
Sbjct: 211 F---AHKDR--------------------------------------------------- 270

Query: 242 KAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSGLGNDS 301
                 DVQ+                                                  
Sbjct: 271 ------DVQDE------------------------------------------------- 330

Query: 302 EIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFI 361
                            +++   E   + NR+L  Y GTHNFHNFT++   +DP+A R+I
Sbjct: 331 -----------------TYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYI 390

Query: 362 VSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVN 421
           +        V EG+EF    V GQSFM+HQIRKM+GL VAI++  APES++E++   +  
Sbjct: 391 LEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTE-K 399

Query: 422 INVPTAPEVGLYLDECLFTSYNQKWKDS--HEEVSMKAYEEVAQDFKMKQIYSHIASTEH 481
           ++VP AP +GL L+   F  YNQ++ +   HE +     E     FK + IY  I  TE 
Sbjct: 451 VDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTER 399

Query: 482 KDGAVALWLHSLNHRNY 494
            + ++A WL +L   N+
Sbjct: 511 DERSMAQWLSTLPIHNF 399

[Close]

BLAST of Cla97C05G096390 vs. Swiss-Prot
Match: sp|Q6CC39|PUS1_YARLI (tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 2.7e-46
Identity = 130/425 (30.59%), Postives = 204/425 (48.00%), Query Frame = 0


Query: 57  IAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKG 116
           +A    YCG GY GMQ NP  KTIE D+ +A   AGA+ + +    K+  + R+ARTDKG
Sbjct: 87  VACMIGYCGTGYHGMQLNPPQKTIEGDIFQAFVKAGAISQNNADDPKKSAFMRAARTDKG 146

Query: 117 VSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPV 176
           V A G V+S +  I+    V+++NS+L  Q+R++G  R   +F  +K C  R Y YL+P 
Sbjct: 147 VHAAGNVISLKMIIEDENIVEKINSHLPEQLRVWGVSRTNKAFECRKLCSSRVYEYLMPT 206

Query: 177 FAL---DPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDI 236
           ++     P      + +K     D E                  GK+ +E         +
Sbjct: 207 YSFLNPRPGTVMSEKLLKDGTSPDEE------------------GKKYWE--------SV 266

Query: 237 SSNTGKAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSG 296
           +++               LE    + D        ++ +C +   G E    +E    + 
Sbjct: 267 AAD---------------LESQGVSYDE------WMKRACIDEIKGEETKEVAESEVKTD 326

Query: 297 LGNDSEIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPA 356
              D+  +   ++V    +    F+   E  A+   ILK Y GTHNFHNFT     +DP+
Sbjct: 327 SKTDAATLEKIKAVERRHRE--EFRISGERLAKIREILKIYEGTHNFHNFTLGKAFKDPS 386

Query: 357 ARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGL-AVAIMRNCAPESLIEKA 416
           A R + S   S   +++G E+V  ++ GQSFMLHQIRKM+ + A+++  N  P+ LI + 
Sbjct: 387 AMRTMKSLTCSDPFLIDGTEWVSIKIHGQSFMLHQIRKMISMVALSVRCNADPQKLIPQT 446

Query: 417 LQKDVNINVPTAPEVGLYLDECLFTSYNQKWKD--SHEEVSMKAYEEVAQDFKMKQIYSH 476
            +K   IN+P AP +GL L+  ++ SYN+K +     E V    + +  + FK K IY  
Sbjct: 447 FEK-ARINIPKAPALGLLLERPVYDSYNKKLQGEFGREGVHFDNWNDQIEAFKHKFIYDK 461

[Close]

BLAST of Cla97C05G096390 vs. Swiss-Prot
Match: sp|Q59S63|PUS1_CANAL (tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PUS1 PE=3 SV=2)

HSP 1 Score: 187.6 bits (475), Expect = 3.5e-46
Identity = 127/425 (29.88%), Postives = 198/425 (46.59%), Query Frame = 0


Query: 57  IAIFFAYCGVGYQGM--QKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTD 116
           +A+   YCG GY GM  Q +P  KTIE D+ +A+  AGA+  ++    K+  + R+ARTD
Sbjct: 151 VAVMLGYCGTGYNGMQVQNDPNVKTIEKDIYDAMATAGAISAENAVDLKKSGFQRAARTD 210

Query: 117 KGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLI 176
           KGV A G V+S +  I+ P  ++++N  L  QIRI+G +R T  F+ +K C  R Y YL+
Sbjct: 211 KGVHAAGNVISLKMIIEDPEIINKINDLLPKQIRIWGIQRTTKGFDCRKCCSSRIYEYLL 270

Query: 177 PVFALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTSITDSDIS 236
           P F+L P         K+ L   +ELVK                      +  + + D+ 
Sbjct: 271 PTFSLLP------PKPKSVL---SELVK----------------------EKKLENPDLF 330

Query: 237 SNTGKAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSGL 296
            +  + I+  +  ++++L    T     +Q   +                          
Sbjct: 331 EDDQEGIDWWENVKSKILASGIT----QEQIDSITXXXXXXXXXXXXXXXXXXXXXXXXX 390

Query: 297 GNDSEIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAA 356
                                S++        F  ++K Y GTHNFHNFT     +D +A
Sbjct: 391 XXXXXXXXXXXXXXXXXXXRRSYRISSSRLQHFREVMKQYEGTHNFHNFTVGKPFKDTSA 450

Query: 357 RRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQ 416
            RF++    S   V+EG E++  ++ GQSFMLHQIRKM+ +A  ++R   P  +I    Q
Sbjct: 451 NRFMIKTIVSDPFVIEGTEWISIKIHGQSFMLHQIRKMIAMAALVVRLSLPCGIINNFFQ 510

Query: 417 KDVNINVPTAPEVGLYLDECLFTSYNQK-WKDSHEEVSMKAYEEVAQDFKMKQIYSHIAS 476
               IN+P AP +GL L+  +F  YN K  K  +E +    +++   +FKMK IY  I +
Sbjct: 511 -STKINIPKAPALGLLLENPVFDGYNIKLTKSDYEPIDFTKFDKEMNEFKMKYIYDKIYA 539

Query: 477 TEHKD 479
            E K+
Sbjct: 571 EESKE 539

[Close]

BLAST of Cla97C05G096390 vs. TAIR10
Match: AT1G20370.1 (Pseudouridine synthase family protein)

HSP 1 Score: 523.1 bits (1346), Expect = 2.0e-148
Identity = 280/449 (62.36%), Postives = 338/449 (75.28%), Query Frame = 0


Query: 57  IAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKG 116
           +AI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ AGAVP+ DR   + Y+WARSARTDKG
Sbjct: 60  VAIVFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPDADRNKPRNYEWARSARTDKG 119

Query: 117 VSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPV 176
           VSAVGQVVSGRFY+DPPGFV+RLNS L  QIR+FGYKRV  SF++KKFCDRRRYVYLIPV
Sbjct: 120 VSAVGQVVSGRFYVDPPGFVERLNSKLPDQIRVFGYKRVAPSFSSKKFCDRRRYVYLIPV 179

Query: 177 FALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVE-GLMGKRN--FEVKTSITDSDI 236
           FALDP  H + E V+  LG   E VKC+ECSE+G K+  G+MGK     + K+    SDI
Sbjct: 180 FALDPCVHGEAEMVRTDLG--YEYVKCVECSEKGYKIPLGVMGKDTDCCDTKSLEIQSDI 239

Query: 237 SSNTGKAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMSG 296
           SS+T  AI  +DV +   L  +  ++++N  + VL     + L      ++K+EG E S 
Sbjct: 240 SSSTCDAIG-ADV-KCETLSSSVPSAENNLNSEVLDGADVSAL------SSKAEGMEESN 299

Query: 297 LGNDSEIVVSKESVNGNE-----KPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTK 356
                   ++K  +N  E     K    F YGE+EK RFNRIL  YVG+HNFHNFTTRTK
Sbjct: 300 -------TLAKVEMNNGEGEDMTKSESKFCYGEKEKERFNRILSYYVGSHNFHNFTTRTK 359

Query: 357 AEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESL 416
           A DPAA R+I+SF+A+T + ++G +FVKCEVVGQSFMLHQIRKM+GLAVAIMRN APESL
Sbjct: 360 AADPAANRYILSFNANTVINLDGKDFVKCEVVGQSFMLHQIRKMIGLAVAIMRNYAPESL 419

Query: 417 IEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIY 476
           IE + +KDV INVP APEVGLYLDEC FTSYN+++K SHEEVSM+ Y+EVA++FK K +Y
Sbjct: 420 IEASFKKDVRINVPMAPEVGLYLDECFFTSYNKRFKGSHEEVSMEEYKEVAEEFKWKYVY 479

Query: 477 SHIASTEHKDGAVALWLHSLNHRNYPDLR 498
           SHI S E KDGAVA+WLHSLN RNYPDLR
Sbjct: 480 SHIGSAEEKDGAVAIWLHSLNQRNYPDLR 491

[Close]

BLAST of Cla97C05G096390 vs. TAIR10
Match: AT1G76120.1 (Pseudouridine synthase family protein)

HSP 1 Score: 520.4 bits (1339), Expect = 1.3e-147
Identity = 277/444 (62.39%), Postives = 325/444 (73.20%), Query Frame = 0


Query: 57  IAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKG 116
           +AI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ AGAVPE  RG  K YD+ARSARTDKG
Sbjct: 24  VAIIFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSARTDKG 83

Query: 117 VSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPV 176
           VSAVGQVVSGRF +DP GFV+RLNSNL  QIRIFGYK VT SF++KKFCDRRRYVYL+PV
Sbjct: 84  VSAVGQVVSGRFIVDPLGFVNRLNSNLPNQIRIFGYKHVTPSFSSKKFCDRRRYVYLLPV 143

Query: 177 FALDPNCHRDRESVKASLGSDNELVKCLECSERGRKV-EGLMGK---RNFEVKTSITDSD 236
           FALDP  HRDRE+V ASLG   E VKC ECSERGRK+  GL+GK    NF  K+    SD
Sbjct: 144 FALDPISHRDRETVMASLGPGEEYVKCFECSERGRKIPPGLVGKWKGTNFGTKSLDFQSD 203

Query: 237 ISSNTGKAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTAKSEGTEMS 296
           ISSN   A+  SD++   +          + +   + E+SC +LN  S  T         
Sbjct: 204 ISSNNSSALR-SDIKIEALSSNLAGLCSVDVEVGRIQEDSC-KLNTNSSET--------- 263

Query: 297 GLGNDSEIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDP 356
                              K    F YGE+EK RF+RIL  YVG++NFHNFTTRTKA+DP
Sbjct: 264 -------------------KVKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKADDP 323

Query: 357 AARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKA 416
            A R I+SF A+T + ++GI+F+KCEV+G+SFMLHQIRKMMGLAVAIMRNCA ESLI+ A
Sbjct: 324 TANRQIISFTANTVINLDGIDFIKCEVLGKSFMLHQIRKMMGLAVAIMRNCASESLIQSA 383

Query: 417 LQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAQDFKMKQIYSHIA 476
             KDVNI VP APEVGLYLDEC FTSYN+ ++DSHEEVSM+AY+E A+ FK+K IYSHI 
Sbjct: 384 FSKDVNITVPMAPEVGLYLDECFFTSYNRNFEDSHEEVSMEAYKEEAEAFKLKHIYSHIG 437

Query: 477 STEHKDGAVALWLHSLNHRNYPDL 497
           +TE K G +ALWLHSLN+RNYPDL
Sbjct: 444 ATERKYGNMALWLHSLNYRNYPDL 437

[Close]

BLAST of Cla97C05G096390 vs. TAIR10
Match: AT2G30320.1 (Pseudouridine synthase family protein)

HSP 1 Score: 101.3 bits (251), Expect = 1.8e-21
Identity = 103/388 (26.55%), Postives = 158/388 (40.72%), Query Frame = 0


Query: 57  IAIFFAYCGVGYQGMQ---KNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSART 116
           + I   Y G  Y+G+Q    +P  KTIE +LE A+Y AG + + + G   +  WARS+RT
Sbjct: 65  VVIRIGYVGTDYRGLQIQRDDPSIKTIEGELEVAIYKAGGIRDSNYGDLHKIGWARSSRT 124

Query: 117 DKGVSAVGQVVSGRFYI-------DPPGFV--DRLNSNLSPQIRIFGYKRVTASFNAKKF 176
           DKGV ++   +S +  I       DP G V    ++ +L   IR+F        F+ ++ 
Sbjct: 125 DKGVHSLATSISLKMEIPETAWKDDPQGTVLAKCISKHLPENIRVFSVLPSNRRFDPRRE 184

Query: 177 CDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEV 236
           C  R+Y YL+PV  L          +K S  SD       + +E  ++ EG     N+  
Sbjct: 185 CTLRKYSYLLPVDVL---------GIKNSFTSDEIDYHITDFNEILKEFEGEYPFHNYTQ 244

Query: 237 KTSITDSDISSNTGKAIEVSDVQENRVLEGNDTTSDSNKQTSVLVENSCTELNVGSEGTA 296
           ++                     E ++ + N       K     ++ S +E    +    
Sbjct: 245 RSRYRRK---------------SEQKIKQRNGRPPREPKS----IKASESEFREENHIEI 304

Query: 297 KSEGTEMSGLGNDSEIVVSKESVNGNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFT 356
             E           E VV+ +S N        + Y  +E  + +       G H    F 
Sbjct: 305 GEEXXXXXXXXXXXEHVVTPDSDNSQVYSRAKWLYEPDETDKIS-------GAHFRKVFR 364

Query: 357 TRT-KAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNC 416
            R+ K E+                   G  FV+  + G+SFMLHQIRKM+G AVA+ R  
Sbjct: 365 CRSGKLENSL-----------------GFGFVEISIWGESFMLHQIRKMIGTAVAVKREL 400

Query: 417 APESLIEKALQKDVNINVPTAPEVGLYL 432
            P  +I  +L K   I +P AP   L L
Sbjct: 425 LPRDIIRLSLNKFTRIVLPLAPSEVLIL 400

[Close]

BLAST of Cla97C05G096390 vs. TAIR10
Match: AT1G20290.1 (SWI-SNF-related chromatin binding protein)

HSP 1 Score: 68.2 bits (165), Expect = 1.7e-11
Identity = 34/53 (64.15%), Postives = 38/53 (71.70%), Query Frame = 0


Query: 446 SHEEVSMKAYEEVAQD-FKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLR 498
           S  EVS+   +E   D FK K +YSHI S E KDGAVA+WLHSLN RNYPDLR
Sbjct: 82  SWREVSVDDDQEQEHDQFKWKHVYSHIGSAEEKDGAVAIWLHSLNQRNYPDLR 134

[Close]

BLAST of Cla97C05G096390 vs. TAIR10
Match: AT5G35400.2 (Pseudouridine synthase family protein)

HSP 1 Score: 64.3 bits (155), Expect = 2.5e-10
Identity = 43/134 (32.09%), Postives = 67/134 (50.00%), Query Frame = 0


Query: 59  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRY------DWARSAR 118
           I  +Y G  + G QK P   T++  +E++  L G V E+   L K+           + R
Sbjct: 97  IVLSYNGASFDGWQKQPDLHTVQGVVEKS--LGGFVDERKAQLLKKKCKPLEGRVLVAGR 156

Query: 119 TDKGVSAVGQVVSGRFY-----IDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDR 178
           TDKGVSA+ QV S  FY     I+P    D +N + S ++R+    +V+ SF+       
Sbjct: 157 TDKGVSALNQVCS--FYTWRKDIEPIAIEDAINKDASGKLRVVSISKVSRSFHPNFSAKW 216

Query: 179 RRYVYLIPVFALDP 182
           RRY+Y+ P+   +P
Sbjct: 217 RRYLYIFPLDDAEP 226

The following BLAST results are available for this feature:

BLAST of Cla97C05G096390 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008452911.1	1.1e-245	94.76	PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo] >XP_...	[more]
XP_004145528.1	7.8e-244	93.89	PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis sativus] >...	[more]
XP_022975443.1	5.8e-239	91.74	tRNA pseudouridine synthase A [Cucurbita maxima]	[more]
XP_022936288.1	1.6e-236	91.11	tRNA pseudouridine synthase A [Cucurbita moschata]	[more]
XP_023536425.1	7.3e-234	90.43	tRNA pseudouridine synthase A [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cla97C05G096390 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BV07\|A0A1S3BV07_CUCME	7.2e-246	94.76	tRNA pseudouridine synthase A, mitochondrial-like OS=Cucumis melo OX=3656 GN=LOC...	[more]
tr\|E5GCD2\|E5GCD2_CUCME	7.2e-246	94.76	tRNA pseudouridine synthase family protein OS=Cucumis melo subsp. melo OX=412675...	[more]
tr\|A0A0A0L0J9\|A0A0A0L0J9_CUCSA	5.2e-244	93.89	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G652870 PE=4 SV=1	[more]
tr\|A0A2N9FMA8\|A0A2N9FMA8_FAGSY	1.2e-184	75.11	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS15931 PE=4 SV=1	[more]
tr\|A0A2K3NXY5\|A0A2K3NXY5_TRIPR	4.8e-181	74.24	tRNA pseudouridine synthase A mitochondrial-like OS=Trifolium pratense OX=57577 ...	[more]

BLAST of Cla97C05G096390 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9WU56\|TRUA_MOUSE	4.5e-49	31.67	tRNA pseudouridine synthase A OS=Mus musculus OX=10090 GN=Pus1 PE=1 SV=2	[more]
sp\|Q4KM92\|TRUA_RAT	4.5e-49	31.67	tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2	[more]
sp\|Q9Y606\|TRUA_HUMAN	7.1e-47	31.35	tRNA pseudouridine synthase A OS=Homo sapiens OX=9606 GN=PUS1 PE=1 SV=3	[more]
sp\|Q6CC39\|PUS1_YARLI	2.7e-46	30.59	tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) O...	[more]
sp\|Q59S63\|PUS1_CANAL	3.5e-46	29.88	tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876...	[more]

BLAST of Cla97C05G096390 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G20370.1	2.0e-148	62.36	Pseudouridine synthase family protein	[more]
AT1G76120.1	1.3e-147	62.39	Pseudouridine synthase family protein	[more]
AT2G30320.1	1.8e-21	26.55	Pseudouridine synthase family protein	[more]
AT1G20290.1	1.7e-11	64.15	SWI-SNF-related chromatin binding protein	[more]
AT5G35400.2	2.5e-10	32.09	Pseudouridine synthase family protein	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003723	RNA binding
GO:0009982	pseudouridine synthase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0009451	RNA modification
GO:0001522	pseudouridine synthesis

Vocabulary: **INTERPRO**
Term	Definition
IPR020103	PsdUridine_synth_cat_dom_sf
IPR001406	PsdUridine_synth_TruA
IPR020095	PsdUridine_synth_TruA_C
IPR020097	PsdUridine_synth_TruA_a/b_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
biological_process	GO:1990481	mRNA pseudouridine synthesis
biological_process	GO:0031119	tRNA pseudouridine synthesis
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005634	nucleus
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C05G096390.1	Cla97C05G096390.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon 97103 v2
Date Performed: 2019-05-12


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 331..436 e-value: 5.4E-14 score: 52.5
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	G3DSA:3.30.70.660		coord: 299..500 e-value: 1.8E-65 score: 222.3
None	No IPR available	GENE3D	G3DSA:3.30.70.580		coord: 45..165 e-value: 1.3E-36 score: 127.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 27..47
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..49
None	No IPR available	PANTHER	PTHR11142:SF4	TRNA PSEUDOURIDINE SYNTHASE A, MITOCHONDRIAL	coord: 29..497
None	No IPR available	CDD	cd02568	PseudoU_synth_PUS1_PUS2	coord: 59..436 e-value: 5.9107E-106 score: 318.789
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 29..497
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	SSF55120	Pseudouridine synthase	coord: 53..189 coord: 317..446

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cla97C05G096390	Cla007409	Watermelon (97103) v1	wmwmbB207
Cla97C05G096390	Csa4G652870	Cucumber (Chinese Long) v2	cuwmbB310
Cla97C05G096390	MELO3C017001	Melon (DHL92) v3.5.1	mewmbB481
Cla97C05G096390	ClCG05G015300	Watermelon (Charleston Gray)	wcgwmbB229
Cla97C05G096390	CSPI04G26660	Wild cucumber (PI 183967)	cpiwmbB320
Cla97C05G096390	Cucsa.271890	Cucumber (Gy14) v1	cgywmbB472
Cla97C05G096390	CmaCh09G000770	Cucurbita maxima (Rimu)	cmawmbB029
Cla97C05G096390	CmoCh09G000760	Cucurbita moschata (Rifu)	cmowmbB017
Cla97C05G096390	Lsi04G017860	Bottle gourd (USVL1VR-Ls)	lsiwmbB291
Cla97C05G096390	CsGy4G024990	Cucumber (Gy14) v2	cgybwmbB293
Cla97C05G096390	MELO3C017001.2	Melon (DHL92) v3.6.1	medwmbB474
Cla97C05G096390	Carg08650	Silver-seed gourd	carwmbB0108
Cla97C05G096390	CsaV3_4G037020	Cucumber (Chinese Long) v3	cucwmbB317
Cla97C05G096390	Bhi09G002426	Wax gourd	wgowmbB576

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla97C05G096390	Silver-seed gourd	carwmbB0374
Cla97C05G096390	Cucurbita maxima (Rimu)	cmawmbB483
Cla97C05G096390	Cucurbita moschata (Rifu)	cmowmbB464
Cla97C05G096390	Wild cucumber (PI 183967)	cpiwmbB217