Csa4G652870 (gene) Cucumber (Chinese Long) v2

Overview
NameCsa4G652870
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptiontRNA pseudouridine synthase family protein; contains IPR001406 (Pseudouridine synthase I, TruA), IPR020103 (Pseudouridine synthase, catalytic domain)
LocationChr4 : 22842677 .. 22844745 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATCTGTTGGATGGGATGGTCTCTAAAACCCTAATGTCTACTTCACACCATTTCCCTACCTGGCTATAATGGAAGAAGAACCGCCAACTGAAACCAGAAGGTCACCGCCACAACCGTTGATCGAAGAACCCCAACCGAAGAAGCTCAAAATGTCATCTACAACCACCACATCCGACGACGAAGATGTTAATTCCAACTCTGCCGCGCCCAAGAAACAAAGGTACAAGCGCCGCAAGGTTGCCATTTTTTTTGCCTACTGTGGCGTCGGTTACCAAGGGATGCAGAAGAACCCTGGCGCTAAAACCATTGAAGCTGACCTTGAAGAAGCCTTATATCTCGCCGGAGCCGTACCTGAGCAGGACCGAGGGCTCTCGAAGCGCTATGATTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTGAGCGCCGTGGGTCAGGTCGTTTCTGGTCGCTTTTACATCGATCCACCAGGTTTTGTTGATCGTCTCAATTCGAATCTTTCGCCTCAGATTCGAATTTTCGGGTACAAGCGCGTCACAGCGTCATTCAATGCGAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATCCCTGTCTTCGCTCTCGATCCCAATTGCCATCGTGATAGAGAAAGTGTGAAAGCTAGTTTAGGGTCTGATAACGAGCTTGTGAAGTGCTTAGAGTGCTCTGAACGAGGACGCAAGGTCGAAGGCTTGATAGGCAAGCGCAATTTCGAAGTAAAAACTGCGATAACTGAGTCAGACATTTCGTCGAACACTGTTAACGCCATTGAAGTTTCTGATGTTCAAGCAAATGGGGTTTTGGAGGACAGTGCCACGACCTCTGATTCTAACAAACAAACTAGCGTTTTAGTAGAAAACACTGCTACTGAACTCAATGTTGGAAGCGAAGGTACTGCAAAAAGTGAAAGCAGTGAAATGTCTGGATTAGGGAGTGATTCAGAGATTGTTGTAAGCAAAGAGAGCGTTAATGGGAAAGAGAAATCTTCAGGGAGCTTCAAGTACGGCGAGGAGGAGAAGGCTAGGTTCAATAGGATTTTGAAACATTATGTGGGAACTCATAACTTCCACAATTTTACTACTAGAACAAAAGCAGAGGATCCTGCTGCTCGTCGATTCATTGTTTCGTTTGATGCTTCTGCAATCGTCGTGGTTGAAGGCATTGAATTTGTGAAATGTGAAGTTGTAGGGCAGAGTTTCATGCTTCATCAGATCCGTAAGATGATGGGTCTTGCTGTGGCAATCATGAGGAACTGTGCTCCAGAGTCATTGATCGAGAAAGCTTTGCAAAAGTAAGTAATTTCCATTTTCGACAACAATTTGAATTGATTCAATTTGATTTTCATTTGTTTATAGAATAGAATAGAATTGGATTTCACCAACTTCTGGTAATCAAAATTCAAACACAGGGATGTAAATATCAATGTGCCCACTGCACCTGAGGTTGGGCTGTACCTAGATGAGTGCCTATTCACATCATATAATCAAAAGTGGAAAGACACTCATGAAGAGGTATCCATGAAAGCATACGAAGAGGCAGCAGAAGATTTCAAAATGAAACAAATATATTCTCACATTGCTTCCACAGAGCACAAAGATGGAGCCGTGGCCTTATGGTTGCATTCTCTAAATCATCGAAATTATCCAGATTTACGTGTCATTGATGAAGTTGCTAAAATTGCTAAGAACGCAAGTCTTGACATGGAGACGCAAGCTGAAACTTAAAACGGTCTTCTTCCACCTTTGCTTTTATTTAAGCATTAACCATCCATTTGGATACTGAGCGGAAAAATACTTGTTTTTGATGATTTAGTGCTTGTTAAATTGTAAACCTAATCAGATTATGCATAAATTTTGCTTAAGAAAGTGAGCCAAATCCATTAATTATGTCAAGTTCTGATTCCTACCTATGAGTCACATTTTCCTTATAGACCCCTACTGACAAGGATTATAATTTTTCTAAAAAACATAGGCCAACCAGAGTTGTTGTAACTTTGAGAATATTAGATTAGAGGTACAAGATAGAAACTCAAAAATGTTTTTTTGCC

mRNA sequence

ATGGAAGAAGAACCGCCAACTGAAACCAGAAGGTCACCGCCACAACCGTTGATCGAAGAACCCCAACCGAAGAAGCTCAAAATGTCATCTACAACCACCACATCCGACGACGAAGATGTTAATTCCAACTCTGCCGCGCCCAAGAAACAAAGGTACAAGCGCCGCAAGGTTGCCATTTTTTTTGCCTACTGTGGCGTCGGTTACCAAGGGATGCAGAAGAACCCTGGCGCTAAAACCATTGAAGCTGACCTTGAAGAAGCCTTATATCTCGCCGGAGCCGTACCTGAGCAGGACCGAGGGCTCTCGAAGCGCTATGATTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTGAGCGCCGTGGGTCAGGTCGTTTCTGGTCGCTTTTACATCGATCCACCAGGTTTTGTTGATCGTCTCAATTCGAATCTTTCGCCTCAGATTCGAATTTTCGGGTACAAGCGCGTCACAGCGTCATTCAATGCGAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATCCCTGTCTTCGCTCTCGATCCCAATTGCCATCGTGATAGAGAAAGTGTGAAAGCTAGTTTAGGGTCTGATAACGAGCTTGTGAAGTGCTTAGAGTGCTCTGAACGAGGACGCAAGGTCGAAGGCTTGATAGGCAAGCGCAATTTCGAAGTAAAAACTGCGATAACTGAGTCAGACATTTCGTCGAACACTGTTAACGCCATTGAAGTTTCTGATGTTCAAGCAAATGGGGTTTTGGAGGACAGTGCCACGACCTCTGATTCTAACAAACAAACTAGCGTTTTAGTAGAAAACACTGCTACTGAACTCAATGTTGGAAGCGAAGGTACTGCAAAAAGTGAAAGCAGTGAAATGTCTGGATTAGGGAGTGATTCAGAGATTGTTGTAAGCAAAGAGAGCGTTAATGGGAAAGAGAAATCTTCAGGGAGCTTCAAGTACGGCGAGGAGGAGAAGGCTAGGTTCAATAGGATTTTGAAACATTATGTGGGAACTCATAACTTCCACAATTTTACTACTAGAACAAAAGCAGAGGATCCTGCTGCTCGTCGATTCATTGTTTCGTTTGATGCTTCTGCAATCGTCGTGGTTGAAGGCATTGAATTTGTGAAATGTGAAGTTGTAGGGCAGAGTTTCATGCTTCATCAGATCCGTAAGATGATGGGTCTTGCTGTGGCAATCATGAGGAACTGTGCTCCAGAGTCATTGATCGAGAAAGCTTTGCAAAAGGATGTAAATATCAATGTGCCCACTGCACCTGAGGTTGGGCTGTACCTAGATGAGTGCCTATTCACATCATATAATCAAAAGTGGAAAGACACTCATGAAGAGGTATCCATGAAAGCATACGAAGAGGCAGCAGAAGATTTCAAAATGAAACAAATATATTCTCACATTGCTTCCACAGAGCACAAAGATGGAGCCGTGGCCTTATGGTTGCATTCTCTAAATCATCGAAATTATCCAGATTTACGTGTCATTGATGAAGTTGCTAAAATTGCTAAGAACGCAAGTCTTGACATGGAGACGCAAGCTGAAACTTAA

Coding sequence (CDS)

ATGGAAGAAGAACCGCCAACTGAAACCAGAAGGTCACCGCCACAACCGTTGATCGAAGAACCCCAACCGAAGAAGCTCAAAATGTCATCTACAACCACCACATCCGACGACGAAGATGTTAATTCCAACTCTGCCGCGCCCAAGAAACAAAGGTACAAGCGCCGCAAGGTTGCCATTTTTTTTGCCTACTGTGGCGTCGGTTACCAAGGGATGCAGAAGAACCCTGGCGCTAAAACCATTGAAGCTGACCTTGAAGAAGCCTTATATCTCGCCGGAGCCGTACCTGAGCAGGACCGAGGGCTCTCGAAGCGCTATGATTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTGAGCGCCGTGGGTCAGGTCGTTTCTGGTCGCTTTTACATCGATCCACCAGGTTTTGTTGATCGTCTCAATTCGAATCTTTCGCCTCAGATTCGAATTTTCGGGTACAAGCGCGTCACAGCGTCATTCAATGCGAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATCCCTGTCTTCGCTCTCGATCCCAATTGCCATCGTGATAGAGAAAGTGTGAAAGCTAGTTTAGGGTCTGATAACGAGCTTGTGAAGTGCTTAGAGTGCTCTGAACGAGGACGCAAGGTCGAAGGCTTGATAGGCAAGCGCAATTTCGAAGTAAAAACTGCGATAACTGAGTCAGACATTTCGTCGAACACTGTTAACGCCATTGAAGTTTCTGATGTTCAAGCAAATGGGGTTTTGGAGGACAGTGCCACGACCTCTGATTCTAACAAACAAACTAGCGTTTTAGTAGAAAACACTGCTACTGAACTCAATGTTGGAAGCGAAGGTACTGCAAAAAGTGAAAGCAGTGAAATGTCTGGATTAGGGAGTGATTCAGAGATTGTTGTAAGCAAAGAGAGCGTTAATGGGAAAGAGAAATCTTCAGGGAGCTTCAAGTACGGCGAGGAGGAGAAGGCTAGGTTCAATAGGATTTTGAAACATTATGTGGGAACTCATAACTTCCACAATTTTACTACTAGAACAAAAGCAGAGGATCCTGCTGCTCGTCGATTCATTGTTTCGTTTGATGCTTCTGCAATCGTCGTGGTTGAAGGCATTGAATTTGTGAAATGTGAAGTTGTAGGGCAGAGTTTCATGCTTCATCAGATCCGTAAGATGATGGGTCTTGCTGTGGCAATCATGAGGAACTGTGCTCCAGAGTCATTGATCGAGAAAGCTTTGCAAAAGGATGTAAATATCAATGTGCCCACTGCACCTGAGGTTGGGCTGTACCTAGATGAGTGCCTATTCACATCATATAATCAAAAGTGGAAAGACACTCATGAAGAGGTATCCATGAAAGCATACGAAGAGGCAGCAGAAGATTTCAAAATGAAACAAATATATTCTCACATTGCTTCCACAGAGCACAAAGATGGAGCCGTGGCCTTATGGTTGCATTCTCTAAATCATCGAAATTATCCAGATTTACGTGTCATTGATGAAGTTGCTAAAATTGCTAAGAACGCAAGTCTTGACATGGAGACGCAAGCTGAAACTTAA

Protein sequence

MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNAIEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEIVVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQAET*
Homology
[Close]
BLAST of Csa4G652870 vs. Swiss-Prot
Match: PUS1_SCHPO (tRNA pseudouridine synthase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=pus1 PE=1 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 7.5e-49
Identity = 138/475 (29.05%), Postives = 219/475 (46.11%), Query Frame = 1 

Query: 50  QRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWAR 109
           ++  +RKVA    YCG GY GMQ NP +KTIE DL +A   AGAV   +    K+   AR
Sbjct: 39  EKKPKRKVACLVGYCGSGYHGMQLNPPSKTIEGDLFDAFVKAGAVSSYNADDPKKVALAR 98

Query: 110 SARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRR 169
           +ARTDKGV A G V+S +  ++    ++++N +L P IR++   R   SFN + +C+ R 
Sbjct: 99  AARTDKGVHAAGNVISLKLIMEDEKLIEKVNEHLPPSIRLWDVIRTINSFNPRTYCESRI 158

Query: 170 YVYLIPVFALDP--------NCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRN 229
           Y Y++P +A  P        NC      + A      E +     ++  R +    GK  
Sbjct: 159 YEYMVPTYAFVPPKPSSILGNCIMKNSPMPA------EPINKENINQLSRSLFYEEGKEF 218

Query: 230 FEVKTAITESDISSNTVNAIEVSD----VQANGVLEDSATTSDSNKQTSVLVENTATELN 289
           ++      + DI++  + ++   D    V        +A  +  N + S    +  T  +
Sbjct: 219 WD------DYDIAAKEILSLYEQDPEGFVNPYSKRGAAALANSENNKGSEAGVSAKTNPD 278

Query: 290 VGSEGTA------KSESSEMSGLGS--DSEIVVSKESVNGKEKSSGSFKYGEEEKARFNR 349
           + S+ +A      K +S E    GS  D    + +   + +     +++   +  +    
Sbjct: 279 MDSDSSAIVNEFLKPDSVEDESAGSKIDPSYRLERALKHIEVLKLKNYRISADRLSVIRE 338

Query: 350 ILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASAIVVVEGIEFVKCEVVGQSFMLHQI 409
            L  YVG HNFHNFT         + R I SF AS   ++   E++ C+V GQSFMLHQI
Sbjct: 339 TLNQYVGVHNFHNFTVGQAFHQKNSNRVIRSFTASDPFMIGDTEWISCKVHGQSFMLHQI 398

Query: 410 RKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKW--KDTH 469
           RKM+ LA+ ++R   P   I+ A +K   IN+P  P  GL L+   F  YN+    ++  
Sbjct: 399 RKMIALAILVVRTGCPVERIQDAFKK-TKINIPKGPGFGLLLESPFFKGYNEHKAPENNR 458

Query: 470 EEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLRVIDEV 503
           + +    YE+    FK   IY  I   E +      +L  ++  N  D   + ++
Sbjct: 459 DPIDFTKYEQKITAFKHAHIYDKIFLEEARKQVFHCFLSFIDSYNEEDFSYLSDI 500

[Close]
BLAST of Csa4G652870 vs. Swiss-Prot
Match: PUS1_CANAL (tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=PUS1 PE=3 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 6.4e-40
Identity = 121/377 (32.10%), Postives = 188/377 (49.87%), Query Frame = 1 

Query: 103 KRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAK 162
           K+  + R+ARTDKGV A G V+S +  I+ P  ++++N  L  QIRI+G +R T  F+ +
Sbjct: 37  KKSGFQRAARTDKGVHAAGNVISLKMIIEDPEIINKINDLLPKQIRIWGIQRATKGFDCR 96

Query: 163 KFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNF 222
           K C  R Y YL+P F+L P      +SV + L  + EL +  +  E  +  EG+    N 
Sbjct: 97  KCCSSRIYEYLLPTFSLLP---PKPKSVLSELVKEKEL-ENPDLFEDDQ--EGIDWWEN- 156

Query: 223 EVKTAITESDISSNTVNAIEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEG 282
            VK+ I  S I+   +               DS T+S   +Q          + +   + 
Sbjct: 157 -VKSKILASGITQEQI---------------DSITSSYDEQQ---------QQQHHQQQQ 216

Query: 283 TAKSESSEMSGLGSDSEIVVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHN 342
            A  E  ++S L S ++++   +++  + + S        +  R     K Y GTHNFHN
Sbjct: 217 QADEEERDISEL-SFTKLIKQIKTIENQSRRSYRISSSRLQHFREVM--KQYEGTHNFHN 276

Query: 343 FTTRTKAEDPAARRFIVSFDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRN 402
           FT     +D +A RF++    S   V+EG E++  ++ GQSFMLHQIRKM+ +A  ++R 
Sbjct: 277 FTVGKPFKDTSANRFMIKTIVSDPFVIEGTEWISIKIHGQSFMLHQIRKMIAMAALVVRL 336

Query: 403 CAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQK-WKDTHEEVSMKAYEEAAED 462
             P  +I    Q    IN+P AP +GL L+  +F  YN K  K  +E +    +++   +
Sbjct: 337 SLPCGIINNFFQ-STKINIPKAPALGLLLENPVFDGYNIKLTKSDYEPIDFTKFDKEMNE 377

Query: 463 FKMKQIYSHIASTEHKD 479
           FKMK IY  I + E K+
Sbjct: 397 FKMKYIYDKIYAEESKE 377

[Close]
BLAST of Csa4G652870 vs. Swiss-Prot
Match: TRUA_MOUSE (tRNA pseudouridine synthase A, mitochondrial OS=Mus musculus GN=Pus1 PE=1 SV=2)

HSP 1 Score: 137.9 bits (346), Expect = 3.2e-31
Identity = 74/180 (41.11%), Postives = 108/180 (60.00%), Query Frame = 1 

Query: 316 SFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASAIVVVEGIEFV 375
           S++   E   + NR+L  Y GTHNFHNFT++    +P+ARR+I+        V EG+EF 
Sbjct: 217 SYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFA 276

Query: 376 KCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECL 435
             +V GQSFM+HQIRKM+GL VAI++  APES++E++  ++  ++VP AP +GL L+   
Sbjct: 277 VIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEE-KVDVPKAPGLGLVLERVH 336

Query: 436 FTSYNQKW--KDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNY 494
           F  YNQ++     HE +     E     FK + IY  I STE  + ++A WL++L   N+
Sbjct: 337 FEKYNQRFGGDGLHEPLDWTQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNF 395


HSP 2 Score: 120.2 bits (300), Expect = 6.9e-26
Identity = 77/210 (36.67%), Postives = 107/210 (50.95%), Query Frame = 1 

Query: 5   PPTETRRSPPQPLIEEPQPK-KLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAY 64
           PP    + PP     +P  K +       T    + V         ++  +RK+ +  AY
Sbjct: 29  PPMAGNKVPPALASHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPRKLPKRKIVLLMAY 88

Query: 65  CGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 124
            G GY GMQ+N G+   +TIE DL  AL  AG +PE      ++  + R ARTDKGVSA 
Sbjct: 89  SGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAA 148

Query: 125 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 184
           GQVVS + ++     +D++NS+L   IRI G KRVT  FN+K  CD R Y Y++P FA  
Sbjct: 149 GQVVSLKVWLIDD-ILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAF- 208

Query: 185 PNCHRDR----ESVKASLGSDNELVKCLEC 207
              H+DR    ES + S  +  ++ + L C
Sbjct: 209 --AHKDRDVQDESYRLSAETLQQVNRLLAC 234

[Close]
BLAST of Csa4G652870 vs. Swiss-Prot
Match: TRUA_RAT (tRNA pseudouridine synthase A, mitochondrial OS=Rattus norvegicus GN=Pus1 PE=1 SV=2)

HSP 1 Score: 137.5 bits (345), Expect = 4.2e-31
Identity = 74/180 (41.11%), Postives = 108/180 (60.00%), Query Frame = 1 

Query: 316 SFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASAIVVVEGIEFV 375
           S++   E   + NR+L  Y GTHNFHNFT++    +P+ARR+I+        V EG+EF 
Sbjct: 217 SYRLSAETLQQVNRLLGCYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFA 276

Query: 376 KCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECL 435
             +V GQSFM+HQIRKM+GL VAI++  APES++E++  ++  ++VP AP +GL L+   
Sbjct: 277 VIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEE-KVDVPKAPGLGLVLERVH 336

Query: 436 FTSYNQKW--KDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNY 494
           F  YNQ++     HE +     E     FK + IY  I STE  + ++A WL++L   N+
Sbjct: 337 FEKYNQRFGSDGLHEPLDWAQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNF 395


HSP 2 Score: 119.0 bits (297), Expect = 1.5e-25
Identity = 69/164 (42.07%), Postives = 95/164 (57.93%), Query Frame = 1 

Query: 50  QRYKRRKVAIFFAYCGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEQDRGLSKRYD 109
           ++  +RK+ +  AY G GY GMQ+N G+   +TIE DL  AL  AG +PE      ++  
Sbjct: 75  RKLPKRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMS 134

Query: 110 WARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCD 169
           + R ARTDKGVSA GQVVS + ++     +D++NS+L   IRI G KRVT  FN+K  CD
Sbjct: 135 FQRCARTDKGVSAAGQVVSLKVWLIDD-ILDKINSHLPSHIRILGLKRVTGGFNSKNKCD 194

Query: 170 RRRYVYLIPVFALDPNCHRDR----ESVKASLGSDNELVKCLEC 207
            R Y Y++P FA     H+DR    ES + S  +  ++ + L C
Sbjct: 195 ARTYCYMLPTFAF---AHKDRDVQDESYRLSAETLQQVNRLLGC 234

[Close]
BLAST of Csa4G652870 vs. Swiss-Prot
Match: TRUA_HUMAN (tRNA pseudouridine synthase A, mitochondrial OS=Homo sapiens GN=PUS1 PE=1 SV=3)

HSP 1 Score: 132.9 bits (333), Expect = 1.0e-29
Identity = 72/180 (40.00%), Postives = 105/180 (58.33%), Query Frame = 1 

Query: 316 SFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASAIVVVEGIEFV 375
           +++   E   + NR+L  Y GTHNFHNFT++   +DP+A R+I+        V EG+EF 
Sbjct: 221 TYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFA 280

Query: 376 KCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECL 435
              V GQSFM+HQIRKM+GL VAI++  APES++E++   +  ++VP AP +GL L+   
Sbjct: 281 VIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTE-KVDVPKAPGLGLVLERVH 340

Query: 436 FTSYNQKWKD--THEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNY 494
           F  YNQ++ +   HE +     E     FK + IY  I  TE  + ++A WL +L   N+
Sbjct: 341 FEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNF 399


HSP 2 Score: 119.4 bits (298), Expect = 1.2e-25
Identity = 65/152 (42.76%), Postives = 90/152 (59.21%), Query Frame = 1 

Query: 40  VNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPE 99
           + S     ++++  +RK+ +  AY G GY GMQ+N G+   KTIE DL  AL  +G +PE
Sbjct: 69  LKSGGDEERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPE 128

Query: 100 QDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVT 159
                 ++  + R ARTDKGVSA GQVVS + ++     ++++NS+L   IRI G KRVT
Sbjct: 129 NHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDD-ILEKINSHLPSHIRILGLKRVT 188

Query: 160 ASFNAKKFCDRRRYVYLIPVFALDPNCHRDRE 189
             FN+K  CD R Y YL+P FA     H+DR+
Sbjct: 189 GGFNSKNRCDARTYCYLLPTFAF---AHKDRD 216

[Close]
BLAST of Csa4G652870 vs. TrEMBL
Match: A0A0A0L0J9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G652870 PE=4 SV=1)

HSP 1 Score: 1030.0 bits (2662), Expect = 9.9e-298
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 1 

Query: 1   MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60
           MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNA
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNA 240

Query: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEI 300
           IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEI 300

Query: 301 VVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQAET 520
           VALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQAET 519

[Close]
BLAST of Csa4G652870 vs. TrEMBL
Match: E5GCD2_CUCME (tRNA pseudouridine synthase family protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 8.9e-291
Identity = 506/519 (97.50%), Postives = 509/519 (98.07%), Query Frame = 1 

Query: 1   MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60
           MEEEPPTETRRSPPQPLIEEP PKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGL+GKRNFEVKTAITESDISSN VNA
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240

Query: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEI 300
           IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATEL V SEG AKSESSEMSGLG+DSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300

Query: 301 VVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNGKEK SGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDAS  VVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEE AEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQAET 520
           VALWLHSLNHRNYPDLRVIDEVAKIAKN S+DMETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 519

[Close]
BLAST of Csa4G652870 vs. TrEMBL
Match: A0A0L9TQX3_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g245700 PE=4 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 4.9e-196
Identity = 358/524 (68.32%), Postives = 416/524 (79.39%), Query Frame = 1 

Query: 3   EEPPTETRRS---PPQPLI-EEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVA 62
           E P T    S   PP PL  +EP+PKKLKMS  TTTSDDE+  +  A   K+RYKRRKVA
Sbjct: 2   ENPDTALTTSSLPPPPPLAADEPEPKKLKMS--TTTSDDEECKT--APGTKKRYKRRKVA 61

Query: 63  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 122
           IFFAYCGVGYQGMQKNPGAKTIE DLEEALY++GAVPE DRG+ KRYDWARSARTDKGVS
Sbjct: 62  IFFAYCGVGYQGMQKNPGAKTIEGDLEEALYVSGAVPEHDRGIPKRYDWARSARTDKGVS 121

Query: 123 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 182
           AVGQVVSGRFYIDPPG VDRLNSNL  QIRIFGYKRVT SF+AKKFCDRRRYVYLIPVFA
Sbjct: 122 AVGQVVSGRFYIDPPGLVDRLNSNLPSQIRIFGYKRVTGSFSAKKFCDRRRYVYLIPVFA 181

Query: 183 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSN-- 242
           LDP+CHRDRE+V ASLGS NELVKCLECSERGRKV GL+G R   ++    + D  SN  
Sbjct: 182 LDPSCHRDRETVMASLGSTNELVKCLECSERGRKVVGLVGNRKHNLELEAMDVDAGSNRD 241

Query: 243 -TVNAIEVSDVQAN-GVLEDSATTSDSNKQTS--VLVENTATELNVGSEGTAKSESSEMS 302
             ++++   DV+ +    +D+    +S   T   VLV+ T  E  + +  T + E   ++
Sbjct: 242 VVLDSVVAEDVEVSLSKGDDNHLNGESGIDTKGEVLVDGTNPETGIENMITVQDEDMPLN 301

Query: 303 GLGSDSEIVVSKESVNGKEKSSGS--FKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAE 362
           G   ++  ++ +E +NG +KS+G   F YGE+E+ RFN++L  +VGTHNFHNFTTRTKA+
Sbjct: 302 GESVNNSDIIEEEKMNGVDKSTGGSRFCYGEKERERFNKVLNFFVGTHNFHNFTTRTKAQ 361

Query: 363 DPAARRFIVSFDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIE 422
           DPAARR+I+SF+A+  VVVEGIEFVKCEVVGQSFMLHQIRKM+GLAVAIMRNCAPESLI 
Sbjct: 362 DPAARRYIISFNANTTVVVEGIEFVKCEVVGQSFMLHQIRKMIGLAVAIMRNCAPESLIN 421

Query: 423 KALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSH 482
           KALQ+DVNINVPTAPEVGLYLDEC F+SYNQKWKD+HEEVSMKAYE+ AE+FKMK IYSH
Sbjct: 422 KALQQDVNINVPTAPEVGLYLDECFFSSYNQKWKDSHEEVSMKAYEKEAEEFKMKYIYSH 481

Query: 483 IASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAKNASLDME 515
           I+STE K+G VALWLHSLNHRNYPDLRV++E A    N S D E
Sbjct: 482 ISSTEQKEGTVALWLHSLNHRNYPDLRVVNEEA-TTDNKSADPE 520

[Close]
BLAST of Csa4G652870 vs. TrEMBL
Match: A0A0S3R6B4_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G417100 PE=4 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 4.9e-196
Identity = 358/524 (68.32%), Postives = 416/524 (79.39%), Query Frame = 1 

Query: 3   EEPPTETRRS---PPQPLI-EEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVA 62
           E P T    S   PP PL  +EP+PKKLKMS  TTTSDDE+  +  A   K+RYKRRKVA
Sbjct: 2   ENPDTALTTSSLPPPPPLAADEPEPKKLKMS--TTTSDDEECKT--APGTKKRYKRRKVA 61

Query: 63  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 122
           IFFAYCGVGYQGMQKNPGAKTIE DLEEALY++GAVPE DRG+ KRYDWARSARTDKGVS
Sbjct: 62  IFFAYCGVGYQGMQKNPGAKTIEGDLEEALYVSGAVPEHDRGIPKRYDWARSARTDKGVS 121

Query: 123 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 182
           AVGQVVSGRFYIDPPG VDRLNSNL  QIRIFGYKRVT SF+AKKFCDRRRYVYLIPVFA
Sbjct: 122 AVGQVVSGRFYIDPPGLVDRLNSNLPSQIRIFGYKRVTGSFSAKKFCDRRRYVYLIPVFA 181

Query: 183 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSN-- 242
           LDP+CHRDRE+V ASLGS NELVKCLECSERGRKV GL+G R   ++    + D  SN  
Sbjct: 182 LDPSCHRDRETVMASLGSTNELVKCLECSERGRKVVGLVGNRKHNLELEAMDVDAGSNRD 241

Query: 243 -TVNAIEVSDVQAN-GVLEDSATTSDSNKQTS--VLVENTATELNVGSEGTAKSESSEMS 302
             ++++   DV+ +    +D+    +S   T   VLV+ T  E  + +  T + E   ++
Sbjct: 242 VVLDSVVAEDVEVSLSKGDDNHLNGESGIDTKGEVLVDGTNPETGIENMITVQDEDMPLN 301

Query: 303 GLGSDSEIVVSKESVNGKEKSSGS--FKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAE 362
           G   ++  ++ +E +NG +KS+G   F YGE+E+ RFN++L  +VGTHNFHNFTTRTKA+
Sbjct: 302 GESVNNSDIIEEEKMNGVDKSTGGSRFCYGEKERERFNKVLNFFVGTHNFHNFTTRTKAQ 361

Query: 363 DPAARRFIVSFDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIE 422
           DPAARR+I+SF+A+  VVVEGIEFVKCEVVGQSFMLHQIRKM+GLAVAIMRNCAPESLI 
Sbjct: 362 DPAARRYIISFNANTTVVVEGIEFVKCEVVGQSFMLHQIRKMIGLAVAIMRNCAPESLIN 421

Query: 423 KALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSH 482
           KALQ+DVNINVPTAPEVGLYLDEC F+SYNQKWKD+HEEVSMKAYE+ AE+FKMK IYSH
Sbjct: 422 KALQQDVNINVPTAPEVGLYLDECFFSSYNQKWKDSHEEVSMKAYEKEAEEFKMKYIYSH 481

Query: 483 IASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAKNASLDME 515
           I+STE K+G VALWLHSLNHRNYPDLRV++E A    N S D E
Sbjct: 482 ISSTEQKEGTVALWLHSLNHRNYPDLRVVNEEA-TTDNKSADPE 520

[Close]
BLAST of Csa4G652870 vs. TrEMBL
Match: V7CDC1_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G157400g PE=4 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 7.0e-195
Identity = 360/519 (69.36%), Postives = 417/519 (80.35%), Query Frame = 1 

Query: 7   TETRRSPPQPLI-EEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCG 66
           T +   PP PL  EEP+PKKLKMS   TTSDDE+  +   A  K+RYKRRK+AIFFAYCG
Sbjct: 9   TTSSLPPPPPLAAEEPEPKKLKMS---TTSDDEECTTAPGA--KKRYKRRKIAIFFAYCG 68

Query: 67  VGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVS 126
           VGYQGMQKNPGAKTIE DLEEALY++GAVPE DRG+ KRYDWARSARTDKGVSAVGQVVS
Sbjct: 69  VGYQGMQKNPGAKTIEGDLEEALYVSGAVPEHDRGIPKRYDWARSARTDKGVSAVGQVVS 128

Query: 127 GRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHR 186
           GRFYIDPPG VDRLNSNL  QIRIFGYKRVT SFNAKKFCDRRRYVYLIPVFALDP CHR
Sbjct: 129 GRFYIDPPGLVDRLNSNLHSQIRIFGYKRVTGSFNAKKFCDRRRYVYLIPVFALDPCCHR 188

Query: 187 DRESVKASLGSDNELVKCLECSERGRKVEGLIGKR--NFEVKTAITESDISSNT---VNA 246
           DRE+V ASLGS NELVKCLECSERGRKV GL+G R  N E++    E+ ISSNT   +++
Sbjct: 189 DRETVMASLGSTNELVKCLECSERGRKVVGLVGNRKHNLELEAMDVEAGISSNTDVVLDS 248

Query: 247 IEVSDVQAN-GVLEDSATTSDS--NKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSD 306
           +   DV+A+    +D+    +S  + +  VLV+ T  +  +      + E   ++G   +
Sbjct: 249 VVTGDVEASLSKGDDNHLNGESGIDNKGKVLVDGTNLKTGIEKGIPVQDEVMPLNGESVN 308

Query: 307 SEIVVSKESVNGKEKS-SGS-FKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAAR 366
           +  ++ +E +NG++KS SGS F YGE+E+ RFN+IL  +VGTHNFHNFTTRTKAEDPAAR
Sbjct: 309 NSDIIEEEKMNGEDKSTSGSMFCYGEKERERFNKILNCFVGTHNFHNFTTRTKAEDPAAR 368

Query: 367 RFIVSFDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQK 426
           R+I+SF+A+    VEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLI KALQ+
Sbjct: 369 RYIISFNANTAFAVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLINKALQQ 428

Query: 427 DVNINVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTE 486
           DVNINVPTAPEVGLYLDEC F+SYNQKWKD+HEE+SMKAYE+ AE+FKMK IYSHI+ TE
Sbjct: 429 DVNINVPTAPEVGLYLDECFFSSYNQKWKDSHEELSMKAYEKEAEEFKMKYIYSHISFTE 488

Query: 487 HKDGAVALWLHSLNHRNYPDLRVIDEVAKIAKNASLDME 515
            K+G VALWLHSLNHRNYPDLRV++E A I  N S D E
Sbjct: 489 QKEGTVALWLHSLNHRNYPDLRVVNEEA-ITDNKSADPE 521

[Close]
BLAST of Csa4G652870 vs. TAIR10
Match: AT1G20370.1 (AT1G20370.1 Pseudouridine synthase family protein)

HSP 1 Score: 560.5 bits (1443), Expect = 1.1e-159
Identity = 295/503 (58.65%), Postives = 365/503 (72.56%), Query Frame = 1 

Query: 1   MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVN---SNSAAPKKQRYKRRKV 60
           ME + P   +         EP  KKLK+S+T   +D   V    SN A  +  +Y+RRKV
Sbjct: 1   MENQEPDIEKLPRDDVPDREPDHKKLKISTTGQETDSSAVTTGGSNVAERRAPKYRRRKV 60

Query: 61  AIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGV 120
           AI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ AGAVP+ DR   + Y+WARSARTDKGV
Sbjct: 61  AIVFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPDADRNKPRNYEWARSARTDKGV 120

Query: 121 SAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVF 180
           SAVGQVVSGRFY+DPPGFV+RLNS L  QIR+FGYKRV  SF++KKFCDRRRYVYLIPVF
Sbjct: 121 SAVGQVVSGRFYVDPPGFVERLNSKLPDQIRVFGYKRVAPSFSSKKFCDRRRYVYLIPVF 180

Query: 181 ALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVE-GLIGKRN--FEVKTAITESDIS 240
           ALDP  H + E V+  LG   E VKC+ECSE+G K+  G++GK     + K+   +SDIS
Sbjct: 181 ALDPCVHGEAEMVRTDLGY--EYVKCVECSEKGYKIPLGVMGKDTDCCDTKSLEIQSDIS 240

Query: 241 SNTVNAIEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGL 300
           S+T +AI  +DV+    L  S  ++++N  + VL     + L+  +EG  +S +      
Sbjct: 241 SSTCDAIG-ADVKCE-TLSSSVPSAENNLNSEVLDGADVSALSSKAEGMEESNTL----- 300

Query: 301 GSDSEIVVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAA 360
              +++ ++        KS   F YGE+EK RFNRIL +YVG+HNFHNFTTRTKA DPAA
Sbjct: 301 ---AKVEMNNGEGEDMTKSESKFCYGEKEKERFNRILSYYVGSHNFHNFTTRTKAADPAA 360

Query: 361 RRFIVSFDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQ 420
            R+I+SF+A+ ++ ++G +FVKCEVVGQSFMLHQIRKM+GLAVAIMRN APESLIE + +
Sbjct: 361 NRYILSFNANTVINLDGKDFVKCEVVGQSFMLHQIRKMIGLAVAIMRNYAPESLIEASFK 420

Query: 421 KDVNINVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIAST 480
           KDV INVP APEVGLYLDEC FTSYN+++K +HEEVSM+ Y+E AE+FK K +YSHI S 
Sbjct: 421 KDVRINVPMAPEVGLYLDECFFTSYNKRFKGSHEEVSMEEYKEVAEEFKWKYVYSHIGSA 480

Query: 481 EHKDGAVALWLHSLNHRNYPDLR 498
           E KDGAVA+WLHSLN RNYPDLR
Sbjct: 481 EEKDGAVAIWLHSLNQRNYPDLR 491

[Close]
BLAST of Csa4G652870 vs. TAIR10
Match: AT1G76120.1 (AT1G76120.1 Pseudouridine synthase family protein)

HSP 1 Score: 534.6 bits (1376), Expect = 6.6e-152
Identity = 283/470 (60.21%), Postives = 339/470 (72.13%), Query Frame = 1 

Query: 31  TTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYL 90
           TTTT ++ D++ +    + Q     KVAI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ 
Sbjct: 3   TTTTDEEIDLSCDGGVTEPQ-----KVAIIFAFCGVGYQGMQKNPGAKTIEGELEEALFH 62

Query: 91  AGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIF 150
           AGAVPE  RG  K YD+ARSARTDKGVSAVGQVVSGRF +DP GFV+RLNSNL  QIRIF
Sbjct: 63  AGAVPESIRGKPKLYDFARSARTDKGVSAVGQVVSGRFIVDPLGFVNRLNSNLPNQIRIF 122

Query: 151 GYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECSERG 210
           GYK VT SF++KKFCDRRRYVYL+PVFALDP  HRDRE+V ASLG   E VKC ECSERG
Sbjct: 123 GYKHVTPSFSSKKFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERG 182

Query: 211 RKV-EGLIGK---RNFEVKTAITESDISSNTVNAIEVSDVQANGVLEDSATTSDSNKQTS 270
           RK+  GL+GK    NF  K+   +SDISSN  +A+  SD++   +  + A     + +  
Sbjct: 183 RKIPPGLVGKWKGTNFGTKSLDFQSDISSNNSSALR-SDIKIEALSSNLAGLCSVDVEVG 242

Query: 271 VLVENTATELNVGSEGTAKSESSEMSGLGSDSEIVVSKESVNGKEKSSGSFKYGEEEKAR 330
            + E++  +LN  S  T                            K    F YGE+EK R
Sbjct: 243 RIQEDSC-KLNTNSSET----------------------------KVKSKFCYGEKEKER 302

Query: 331 FNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASAIVVVEGIEFVKCEVVGQSFML 390
           F+RIL  YVG++NFHNFTTRTKA+DP A R I+SF A+ ++ ++GI+F+KCEV+G+SFML
Sbjct: 303 FSRILSCYVGSYNFHNFTTRTKADDPTANRQIISFTANTVINLDGIDFIKCEVLGKSFML 362

Query: 391 HQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDT 450
           HQIRKMMGLAVAIMRNCA ESLI+ A  KDVNI VP APEVGLYLDEC FTSYN+ ++D+
Sbjct: 363 HQIRKMMGLAVAIMRNCASESLIQSAFSKDVNITVPMAPEVGLYLDECFFTSYNRNFEDS 422

Query: 451 HEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDL 497
           HEEVSM+AY+E AE FK+K IYSHI +TE K G +ALWLHSLN+RNYPDL
Sbjct: 423 HEEVSMEAYKEEAEAFKLKHIYSHIGATERKYGNMALWLHSLNYRNYPDL 437

[Close]
BLAST of Csa4G652870 vs. TAIR10
Match: AT2G30320.1 (AT2G30320.1 Pseudouridine synthase family protein)

HSP 1 Score: 128.3 bits (321), Expect = 1.4e-29
Identity = 122/427 (28.57%), Postives = 182/427 (42.62%), Query Frame = 1 

Query: 23  PKKLKMSST-----TTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQ---KN 82
           P  L+ SS+     ++TS     + N  A K + Y+++KV I   Y G  Y+G+Q    +
Sbjct: 26  PALLRFSSSAAFQPSSTSLSPPSSDNFLADKWESYRKKKVVIRIGYVGTDYRGLQIQRDD 85

Query: 83  PGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYI---- 142
           P  KTIE +LE A+Y AG + + + G   +  WARS+RTDKGV ++   +S +  I    
Sbjct: 86  PSIKTIEGELEVAIYKAGGIRDSNYGDLHKIGWARSSRTDKGVHSLATSISLKMEIPETA 145

Query: 143 ---DPPGFV--DRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHR 202
              DP G V    ++ +L   IR+F        F+ ++ C  R+Y YL+PV  L      
Sbjct: 146 WKDDPQGTVLAKCISKHLPENIRVFSVLPSNRRFDPRRECTLRKYSYLLPVDVL------ 205

Query: 203 DRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNAIEVSD 262
               +K S  SD       + +E  ++ EG     N+  ++               E   
Sbjct: 206 ---GIKNSFTSDEIDYHITDFNEILKEFEGEYPFHNYTQRSRYRRKS---------EQKI 265

Query: 263 VQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEIVVSKE 322
            Q NG        S    ++    EN    + +G E     E  E+ G     E VV+ +
Sbjct: 266 KQRNG-RPPREPKSIKASESEFREEN---HIEIGEE----EEEKEVDG--ESDEHVVTPD 325

Query: 323 SVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRT-KAEDPAARRFIVSFDAS 382
           S N +  S   + Y  +E  + +       G H    F  R+ K E+             
Sbjct: 326 SDNSQVYSRAKWLYEPDETDKIS-------GAHFRKVFRCRSGKLENSL----------- 385

Query: 383 AIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTA 432
                 G  FV+  + G+SFMLHQIRKM+G AVA+ R   P  +I  +L K   I +P A
Sbjct: 386 ------GFGFVEISIWGESFMLHQIRKMIGTAVAVKRELLPRDIIRLSLNKFTRIVLPLA 400

[Close]
BLAST of Csa4G652870 vs. TAIR10
Match: AT1G20290.1 (AT1G20290.1 SWI-SNF-related chromatin binding protein)

HSP 1 Score: 68.9 bits (167), Expect = 1.0e-11
Identity = 36/62 (58.06%), Postives = 39/62 (62.90%), Query Frame = 1 

Query: 437 TSYNQKWKDTHEEVSMKAYEEAAED-FKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPD 496
           TS    W+    EVS+   +E   D FK K +YSHI S E KDGAVA+WLHSLN RNYPD
Sbjct: 77  TSARISWR----EVSVDDDQEQEHDQFKWKHVYSHIGSAEEKDGAVAIWLHSLNQRNYPD 134

Query: 497 LR 498
           LR
Sbjct: 137 LR 134

[Close]
BLAST of Csa4G652870 vs. NCBI nr
Match: gi|449455575|ref|XP_004145528.1| (PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis sativus])

HSP 1 Score: 1030.0 bits (2662), Expect = 1.4e-297
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 1 

Query: 1   MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60
           MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNA
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNA 240

Query: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEI 300
           IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEI 300

Query: 301 VVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQAET 520
           VALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQAET 519

[Close]
BLAST of Csa4G652870 vs. NCBI nr
Match: gi|659104351|ref|XP_008452911.1| (PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo])

HSP 1 Score: 1006.9 bits (2602), Expect = 1.3e-290
Identity = 506/519 (97.50%), Postives = 509/519 (98.07%), Query Frame = 1 

Query: 1   MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60
           MEEEPPTETRRSPPQPLIEEP PKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGL+GKRNFEVKTAITESDISSN VNA
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240

Query: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEI 300
           IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATEL V SEG AKSESSEMSGLG+DSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300

Query: 301 VVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360
           VVSKESVNGKEK SGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS
Sbjct: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDAS  VVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEE AEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQAET 520
           VALWLHSLNHRNYPDLRVIDEVAKIAKN S+DMETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 519

[Close]
BLAST of Csa4G652870 vs. NCBI nr
Match: gi|1012001706|ref|XP_015932762.1| (PREDICTED: tRNA pseudouridine synthase A, mitochondrial isoform X1 [Arachis duranensis])

HSP 1 Score: 695.3 bits (1793), Expect = 8.3e-197
Identity = 363/530 (68.49%), Postives = 421/530 (79.43%), Query Frame = 1 

Query: 13  PPQPLI---EEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCGVGYQ 72
           PP P +   EEP+PKK KMS  T+TSDDE+  +  A   K+RYKRRK+AIFFAYCGVGYQ
Sbjct: 17  PPPPALVVTEEPEPKKQKMS--TSTSDDEECIT--ATGTKKRYKRRKIAIFFAYCGVGYQ 76

Query: 73  GMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFY 132
           GMQKNPGAKTIE D+EEALY++GAVPEQDRG++KRYDWARSARTDKGVSAVGQVVSGRFY
Sbjct: 77  GMQKNPGAKTIEGDMEEALYVSGAVPEQDRGIAKRYDWARSARTDKGVSAVGQVVSGRFY 136

Query: 133 IDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRES 192
           IDPPG VDRLNSNL  QIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDP+CHRDRE+
Sbjct: 137 IDPPGLVDRLNSNLPDQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPSCHRDRET 196

Query: 193 VKASLGSDNELVKCLECSERGRKVEGLIG--KRNFEVKTAITESDISSNTVNA-----IE 252
           V ASLGS NELVKCLECSERGRKV GL+G  K N   +    +S    + VNA     I+
Sbjct: 197 VLASLGSGNELVKCLECSERGRKVVGLVGNAKHNLTQEAMDVDSFRKDDKVNAGLTEDID 256

Query: 253 VSDVQANG------------VLEDS--ATTSDSNKQTSVLVENTATELNVGSEGTAKSES 312
           VS  +  G            V+ D+  + T +S+ +  VLV+N  ++ ++ +    +   
Sbjct: 257 VSLSKGEGKCLNEESGHEDKVVVDNLNSKTEESSNEVKVLVDNVNSKTDLETMVPVQDAD 316

Query: 313 SEMSGLGSDSEIVVSKESVNGKEKSS--GSFKYGEEEKARFNRILKHYVGTHNFHNFTTR 372
           + ++G GS +  +V +E VNG+++ +    F YGEEEK RFNRILK+YVGTHNFHNFTTR
Sbjct: 317 TPVNG-GSVNTAIVEEEKVNGEDRPTKGSGFNYGEEEKERFNRILKYYVGTHNFHNFTTR 376

Query: 373 TKAEDPAARRFIVSFDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPE 432
           TKAEDPAARRFI+SF+A   VVVEG+EFVKCE+VGQSFMLHQIRKM+GLAVAIMRNCAPE
Sbjct: 377 TKAEDPAARRFIISFEAKTTVVVEGMEFVKCEIVGQSFMLHQIRKMVGLAVAIMRNCAPE 436

Query: 433 SLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQ 492
           SLI KALQKDVNINVPTAPEVGLYLDEC F SYNQKWKD+HEE+SMKAYE+ AEDFKMK 
Sbjct: 437 SLINKALQKDVNINVPTAPEVGLYLDECFFASYNQKWKDSHEELSMKAYEKEAEDFKMKY 496

Query: 493 IYSHIASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQ 517
           IYSHIASTE KDG VALWLHSLNHRNYPDLR++DE A I  N   +++ +
Sbjct: 497 IYSHIASTEQKDGTVALWLHSLNHRNYPDLRLVDEEA-ITDNGKTEIKVE 540

[Close]
BLAST of Csa4G652870 vs. NCBI nr
Match: gi|951015038|ref|XP_014510724.1| (PREDICTED: tRNA pseudouridine synthase A, mitochondrial isoform X1 [Vigna radiata var. radiata])

HSP 1 Score: 694.9 bits (1792), Expect = 1.1e-196
Identity = 364/526 (69.20%), Postives = 419/526 (79.66%), Query Frame = 1 

Query: 3   EEPPTETRRS---PPQPLI-EEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVA 62
           E P T    S   PP PL  +EP+PKKLKMS  TTTSDDE+  +  A   K+RYKRRKVA
Sbjct: 2   ENPDTALTTSSLPPPPPLAADEPEPKKLKMS--TTTSDDEECKT--APGTKKRYKRRKVA 61

Query: 63  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 122
           IFFAYCGVGYQGMQKNPGAKTIE DLEEALY++GAVPE DRG+ KRYDWARSARTDKGVS
Sbjct: 62  IFFAYCGVGYQGMQKNPGAKTIEGDLEEALYVSGAVPEHDRGIPKRYDWARSARTDKGVS 121

Query: 123 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 182
           AVGQVVSGRFYIDPPGFVDRLNSNL  QIRIFGYKRVT SFNAKKFCDRRRYVYLIPVFA
Sbjct: 122 AVGQVVSGRFYIDPPGFVDRLNSNLPSQIRIFGYKRVTGSFNAKKFCDRRRYVYLIPVFA 181

Query: 183 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKR--NFEVKTAITESDISSN 242
           LDP+CHRDRE+V ASLGS NELVKCLECSERGRKV GL+G R  N E++    ++ ISSN
Sbjct: 182 LDPSCHRDRETVMASLGSTNELVKCLECSERGRKVVGLVGNRKHNLELEAMDVDAGISSN 241

Query: 243 ---TVNAIEVSDVQAN-GVLEDSATTSDSNKQTS--VLVENTATELNVGSEGTAKSESSE 302
               ++++   DV+ +    +D     +S   T   VLV++T  E  + +    + E   
Sbjct: 242 RDVVLDSVVAEDVEVSLSRGDDDHLNGESGIDTKGEVLVDSTDPETGIENVIPVQDEDMP 301

Query: 303 MSGLGSDSEIVVSKESVNGKEKSSGS--FKYGEEEKARFNRILKHYVGTHNFHNFTTRTK 362
           ++G   ++  +V +E +NG +KS+G   F YGE+E+ RFN+IL  +VGTHNFHNFTTRTK
Sbjct: 302 LNGESVNNSDIVEEEKMNGVDKSTGGSRFCYGEKERERFNKILNFFVGTHNFHNFTTRTK 361

Query: 363 AEDPAARRFIVSFDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESL 422
           A+DPAARR+I+SF+A+  VVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESL
Sbjct: 362 AQDPAARRYIISFNANTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESL 421

Query: 423 IEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIY 482
           I KALQ+DVNINVPTAPEVGLYLDEC F+SYNQKWKD+HE VSMKAYE+ AE+FKMK IY
Sbjct: 422 INKALQQDVNINVPTAPEVGLYLDECFFSSYNQKWKDSHEVVSMKAYEKEAEEFKMKYIY 481

Query: 483 SHIASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAKNASLDME 515
           SHI+STE K+G VALWLHSLNHRNYPDL V++E A I  N S D E
Sbjct: 482 SHISSTEQKEGTVALWLHSLNHRNYPDLHVVNEEA-ITDNKSADPE 522

[Close]
BLAST of Csa4G652870 vs. NCBI nr
Match: gi|920688918|gb|KOM32901.1| (hypothetical protein LR48_Vigan01g245700 [Vigna angularis])

HSP 1 Score: 692.2 bits (1785), Expect = 7.0e-196
Identity = 358/524 (68.32%), Postives = 416/524 (79.39%), Query Frame = 1 

Query: 3   EEPPTETRRS---PPQPLI-EEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVA 62
           E P T    S   PP PL  +EP+PKKLKMS  TTTSDDE+  +  A   K+RYKRRKVA
Sbjct: 2   ENPDTALTTSSLPPPPPLAADEPEPKKLKMS--TTTSDDEECKT--APGTKKRYKRRKVA 61

Query: 63  IFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVS 122
           IFFAYCGVGYQGMQKNPGAKTIE DLEEALY++GAVPE DRG+ KRYDWARSARTDKGVS
Sbjct: 62  IFFAYCGVGYQGMQKNPGAKTIEGDLEEALYVSGAVPEHDRGIPKRYDWARSARTDKGVS 121

Query: 123 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 182
           AVGQVVSGRFYIDPPG VDRLNSNL  QIRIFGYKRVT SF+AKKFCDRRRYVYLIPVFA
Sbjct: 122 AVGQVVSGRFYIDPPGLVDRLNSNLPSQIRIFGYKRVTGSFSAKKFCDRRRYVYLIPVFA 181

Query: 183 LDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSN-- 242
           LDP+CHRDRE+V ASLGS NELVKCLECSERGRKV GL+G R   ++    + D  SN  
Sbjct: 182 LDPSCHRDRETVMASLGSTNELVKCLECSERGRKVVGLVGNRKHNLELEAMDVDAGSNRD 241

Query: 243 -TVNAIEVSDVQAN-GVLEDSATTSDSNKQTS--VLVENTATELNVGSEGTAKSESSEMS 302
             ++++   DV+ +    +D+    +S   T   VLV+ T  E  + +  T + E   ++
Sbjct: 242 VVLDSVVAEDVEVSLSKGDDNHLNGESGIDTKGEVLVDGTNPETGIENMITVQDEDMPLN 301

Query: 303 GLGSDSEIVVSKESVNGKEKSSGS--FKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAE 362
           G   ++  ++ +E +NG +KS+G   F YGE+E+ RFN++L  +VGTHNFHNFTTRTKA+
Sbjct: 302 GESVNNSDIIEEEKMNGVDKSTGGSRFCYGEKERERFNKVLNFFVGTHNFHNFTTRTKAQ 361

Query: 363 DPAARRFIVSFDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIE 422
           DPAARR+I+SF+A+  VVVEGIEFVKCEVVGQSFMLHQIRKM+GLAVAIMRNCAPESLI 
Sbjct: 362 DPAARRYIISFNANTTVVVEGIEFVKCEVVGQSFMLHQIRKMIGLAVAIMRNCAPESLIN 421

Query: 423 KALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSH 482
           KALQ+DVNINVPTAPEVGLYLDEC F+SYNQKWKD+HEEVSMKAYE+ AE+FKMK IYSH
Sbjct: 422 KALQQDVNINVPTAPEVGLYLDECFFSSYNQKWKDSHEEVSMKAYEKEAEEFKMKYIYSH 481

Query: 483 IASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAKNASLDME 515
           I+STE K+G VALWLHSLNHRNYPDLRV++E A    N S D E
Sbjct: 482 ISSTEQKEGTVALWLHSLNHRNYPDLRVVNEEA-TTDNKSADPE 520

The following BLAST results are available for this feature:
BLAST of Csa4G652870 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PUS1_SCHPO7.5e-4929.05tRNA pseudouridine synthase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
PUS1_CANAL6.4e-4032.10tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876... [more]
TRUA_MOUSE3.2e-3141.11tRNA pseudouridine synthase A, mitochondrial OS=Mus musculus GN=Pus1 PE=1 SV=2[more]
TRUA_RAT4.2e-3141.11tRNA pseudouridine synthase A, mitochondrial OS=Rattus norvegicus GN=Pus1 PE=1 S... [more]
TRUA_HUMAN1.0e-2940.00tRNA pseudouridine synthase A, mitochondrial OS=Homo sapiens GN=PUS1 PE=1 SV=3[more]
BLAST of Csa4G652870 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L0J9_CUCSA9.9e-298100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G652870 PE=4 SV=1[more]
E5GCD2_CUCME8.9e-29197.50tRNA pseudouridine synthase family protein OS=Cucumis melo subsp. melo PE=4 SV=1[more]
A0A0L9TQX3_PHAAN4.9e-19668.32Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g245700 PE=4 SV=1[more]
A0A0S3R6B4_PHAAN4.9e-19668.32Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G417100 PE=... [more]
V7CDC1_PHAVU7.0e-19569.36Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G157400g PE=4 SV=1[more]
BLAST of Csa4G652870 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G20370.11.1e-15958.65 Pseudouridine synthase family protein[more]
AT1G76120.16.6e-15260.21 Pseudouridine synthase family protein[more]
AT2G30320.11.4e-2928.57 Pseudouridine synthase family protein[more]
AT1G20290.11.0e-1158.06 SWI-SNF-related chromatin binding protein[more]
BLAST of Csa4G652870 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449455575|ref|XP_004145528.1|1.4e-297100.00PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis sativus][more]
gi|659104351|ref|XP_008452911.1|1.3e-29097.50PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo][more]
gi|1012001706|ref|XP_015932762.1|8.3e-19768.49PREDICTED: tRNA pseudouridine synthase A, mitochondrial isoform X1 [Arachis dura... [more]
gi|951015038|ref|XP_014510724.1|1.1e-19669.20PREDICTED: tRNA pseudouridine synthase A, mitochondrial isoform X1 [Vigna radiat... [more]
gi|920688918|gb|KOM32901.1|7.0e-19668.32hypothetical protein LR48_Vigan01g245700 [Vigna angularis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001406PsdUridine_synth_TruA
IPR020094Pseudouridine synthase I, TruA, N-terminal
IPR020095PsdUridine_synth_TruA_C
IPR020097PsdUridine_synth_TruA_a/b_dom
IPR020103PsdUridine_synth_cat_dom_sf
Vocabulary: Biological Process
TermDefinition
GO:0001522pseudouridine synthesis
GO:0009451RNA modification
Vocabulary: Molecular Function
TermDefinition
GO:0003723RNA binding
GO:0009982pseudouridine synthase activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
cellular_component GO:0005575 cellular_component
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding
Unigene
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU085116cucumber EST collection version 3.0transcribed_cluster
CU098301cucumber EST collection version 3.0transcribed_cluster
CU116741cucumber EST collection version 3.0transcribed_cluster
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa4G652870.1Csa4G652870.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU085116CU085116transcribed_cluster
CU098301CU098301transcribed_cluster
CU116741CU116741transcribed_cluster


InterPro
Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001406Pseudouridine synthase I, TruAPANTHERPTHR11142PSEUDOURIDYLATE SYNTHASEcoord: 7..195
score: 8.0E-176coord: 318..518
score: 8.0E
IPR020094Pseudouridine synthase I, TruA, N-terminalGENE3DG3DSA:3.30.70.580coord: 53..165
score: 1.8
IPR020095Pseudouridine synthase I, TruA, C-terminalGENE3DG3DSA:3.30.70.660coord: 316..437
score: 5.0
IPR020097Pseudouridine synthase I, TruA, alpha/beta domainPFAMPF01416PseudoU_synth_1coord: 331..436
score: 4.7
IPR020103Pseudouridine synthase, catalytic domainunknownSSF55120Pseudouridine synthasecoord: 53..189
score: 4.75E-62coord: 317..446
score: 4.75
NoneNo IPR availablePANTHERPTHR11142:SF4TRNA PSEUDOURIDINE SYNTHASE A, MITOCHONDRIALcoord: 318..518
score: 8.0E-176coord: 7..195
score: 8.0E