MELO3C015861.2 (gene) Melon (DHL92) v3.6.1

BLAST of MELO3C015861.2 vs. NCBI nr
Match: XP_008451396.1 (PREDICTED: isoamylase 2, chloroplastic [Cucumis melo])

HSP 1 Score: 1786.2 bits (4625), Expect = 0.0e+00
Identity = 885/885 (100.00%), Postives = 885/885 (100.00%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
           MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
           VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
           AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300
           SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
           YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
           WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
           NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
           VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
           PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSANA 886
           RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSANA
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSANA 885

BLAST of MELO3C015861.2 vs. NCBI nr
Match: XP_004136188.1 (PREDICTED: isoamylase 2, chloroplastic [Cucumis sativus] >KGN44865.1 hypothetical protein Csa_7G392380 [Cucumis sativus])

HSP 1 Score: 1686.0 bits (4365), Expect = 0.0e+00
Identity = 830/884 (93.89%), Postives = 852/884 (96.38%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
           MATFLSSLVIQPFCV+NCGVTES KLA SDHLTYGQKTKYQ GKMDEARMLAHGE+KVGA
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
           VKSSHRNLSK Y  SGI VGKSGQRLGIGGKSKEQR+VATYLFRTEFGDLVNVFVGK GS
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
            FTVNIE+ SMQLVS DE LLLSW VYRSDSA VT NF+SSPPD TTGATETPFVKTSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLK+PMGVDSGSSEIRSH+KTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300
           SGDGS+NFSIFSS+AES+VLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGAS FVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
           YGYQCKG KSHENQDGLEVSRIVVDPYAKILAPSIPKS  QGLG P KFLGQIS VP FD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
           WDGEV PNLPMEKLFVYRLNV+RFT+DKSSQLPADIAGTFSGLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVI+EVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480

Query: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           TS +GALQGIDDSSYYF NRVANLEE+SALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
           NASFLL+GHHGELLSRPPFVEAIAFDP+LSKTKLVADFWDPQEL SKETRFPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPT+ LKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
           VAFND+RSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQ+RNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
           PQWEDASCKFLAVMLRADKEENESITENPKTRS+IFM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 885
           RVVDTALPFPGFFSSDGELVPMTGSVTYE+QAHSCALFEAKSAN
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 884

BLAST of MELO3C015861.2 vs. NCBI nr
Match: XP_022953509.1 (isoamylase 2, chloroplastic isoform X1 [Cucurbita moschata] >XP_022953510.1 isoamylase 2, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 1513.4 bits (3917), Expect = 0.0e+00
Identity = 744/884 (84.16%), Postives = 801/884 (90.61%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
           MATFLSSL IQPFC +N G TES KLAVSD+ TYGQK  YQSGK+DEARM AHGESKVG 
Sbjct: 1   MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60

Query: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
            KS HRN+ +AY  S I VGKSGQ+ G GGK++E RKVATYLFRTEFGDLV  FVGK GS
Sbjct: 61  TKSLHRNVCRAYAPSRISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120

Query: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
            F  +IEISS++L S DETLLLSW VYRS+SA VT NFQS  P+ T GATETPFV+TSEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
           KF+VELEFDAKH PFYLSF+LK+P+GVDSGSSEI+SHRKTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300
           SGDGSINFSIFSSTAESVVLCLYNDSTSEKP+LELDLDPYINRSGNIWHASFEGASNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
           YGY+C+G K HENQD  EVSRI+VDPYAKIL  SIPK+R QGLG P KFLGQI  VPAF 
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
           WDGEVRPNLPMEKLF+YRLNVK FT+DKSSQLP D AGTFSGLT+KLLH K LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
           EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS++NS+KEMVKELHANGVEVI+EVV+TH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480

Query: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           T++DGALQGIDDS YYF NRVAN E +SALNCN+PIVQQLLLDSLR+WVTEFH+DGFCF+
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540

Query: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
           NAS LLQGHHGELLSRPPFVEAIAFDP+LSKTK++ADFWDPQE V KETRFPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKF +DI++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           +S LASELSWNCGEEGPT+ ++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
           VAF DR+SFNWD LKTDFGTQTTQFI FLSSFRSRRFDLFQSRNFLK ENIDWFDNNQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
           P WED SCKFLAVMLRADKEE+ SITENPK +SDIFMAFNASDQS SVALPEPSEGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 885
           R+VDTAL FPGFFSSDGE VP T  VTY MQAHSC LFEAKS N
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFN 883

BLAST of MELO3C015861.2 vs. NCBI nr
Match: XP_023548355.1 (isoamylase 2, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023548356.1 isoamylase 2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1513.4 bits (3917), Expect = 0.0e+00
Identity = 742/884 (83.94%), Postives = 799/884 (90.38%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
           MATFLSSL IQPFC +N G TES KLAV+D+ TYGQK  YQSGK+DEARM AHGESKVG 
Sbjct: 1   MATFLSSL-IQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60

Query: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
            KS HRN+ +AY  SGI VGKSGQ+ G GGK++E RKVATYLFRTEFGDLV  FVGK GS
Sbjct: 61  TKSLHRNVCRAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120

Query: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
            F VNIEISS++L S DETLLLSW VYRS+SA VT NFQS  P+ T GATE PFV+TSEG
Sbjct: 121 GFMVNIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATEAPFVRTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
           KF+VELEFDAKH PFYLSF+LK+P+GVDSGSSEI+SHRKTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300
           SGDGSINFSIFSSTAESVVLCLYNDSTSEKP+LELDLDPYINRSGNIWHASFEGASNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
           YGY+C+G K HENQD  EVSRI+VDPYAKIL  SIPK+R QGLG P KFLGQI  VPAF 
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
           WDGEVRPNLPMEKLF+YRLNVK FT+DKSSQLP DIAGTFSGLT+KLLH K LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
           EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS++NS+KEMVK LHANGVEVI+EVV+TH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKVLHANGVEVILEVVFTH 480

Query: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           T++DGALQGIDDS YYF NRV N E +SALNCN+PIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVTNSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFI 540

Query: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
           NAS LLQGHHGELLSRPPF EAIAFDP+LSKTK++ADFWDPQE V KE RFPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFAEAIAFDPLLSKTKIIADFWDPQESVPKEIRFPHWKRWAEL 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKF +DI++FFRGEGL+ SLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVRSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           +S LASELSWNCGEEGPT+ ++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
           VAF DR+SFNWD LKTDFGTQTTQFI FLSSFRSRRFDLFQSRNFLK ENIDWFDNNQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
           P WED SCKFLAVMLRADKEE+ SITENPK +SDIFMAFNASDQS SVALPEPSEGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTTWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 885
           R+VDTAL FPGFFSSDGE VP T  +TYEMQAHSC LFEAKS N
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPITYEMQAHSCTLFEAKSFN 883

BLAST of MELO3C015861.2 vs. NCBI nr
Match: XP_022953511.1 (isoamylase 2, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 1492.6 bits (3863), Expect = 0.0e+00
Identity = 738/884 (83.48%), Postives = 793/884 (89.71%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
           MATFLSSL IQPFC +N G TES KLAVSD+ TYGQK  YQSGK+DEARM AHGESKVG 
Sbjct: 1   MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60

Query: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
            KS HRN+ +AY  S I V          GKS+E RKVATYLFRTEFGDLV  FVGK GS
Sbjct: 61  TKSLHRNVCRAYAPSRISV----------GKSEEDRKVATYLFRTEFGDLVYAFVGKRGS 120

Query: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
            F  +IEISS++L S DETLLLSW VYRS+SA VT NFQS  P+ T GATETPFV+TSEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
           KF+VELEFDAKH PFYLSF+LK+P+GVDSGSSEI+SHRKTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300
           SGDGSINFSIFSSTAESVVLCLYNDSTSEKP+LELDLDPYINRSGNIWHASFEGASNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
           YGY+C+G K HENQD  EVSRI+VDPYAKIL  SIPK+R QGLG P KFLGQI  VPAF 
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
           WDGEVRPNLPMEKLF+YRLNVK FT+DKSSQLP D AGTFSGLT+KLLH K LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
           EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS++NS+KEMVKELHANGVEVI+EVV+TH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480

Query: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           T++DGALQGIDDS YYF NRVAN E +SALNCN+PIVQQLLLDSLR+WVTEFH+DGFCF+
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540

Query: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
           NAS LLQGHHGELLSRPPFVEAIAFDP+LSKTK++ADFWDPQE V KETRFPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKF +DI++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           +S LASELSWNCGEEGPT+ ++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
           VAF DR+SFNWD LKTDFGTQTTQFI FLSSFRSRRFDLFQSRNFLK ENIDWFDNNQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
           P WED SCKFLAVMLRADKEE+ SITENPK +SDIFMAFNASDQS SVALPEPSEGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 885
           R+VDTAL FPGFFSSDGE VP T  VTY MQAHSC LFEAKS N
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFN 873

BLAST of MELO3C015861.2 vs. TAIR10
Match: AT1G03310.2 (debranching enzyme 1)

HSP 1 Score: 937.6 bits (2422), Expect = 5.7e-273
Identity = 478/900 (53.11%), Postives = 635/900 (70.56%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
           MA +  S+ I   C++N G+T + K   S  L  G+K K + G   E  M          
Sbjct: 1   MAAWSPSVGIGSCCLNN-GITRTWKFP-SARLFTGRKNKIKLG--SETLMFTRKRFMGDL 60

Query: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSK-----EQRKVATYLFRTEFGDLVNVFV 120
           V S+     ++Y  S I   K+   L     S+     + +KV +Y FRT+ G LV V V
Sbjct: 61  VTSA----LQSYQFSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKV 120

Query: 121 GKTGSAFTVNIEISSMQLVSTDET-LLLSWRVYRSDSAP-VTSNFQSSPPDGTTGATETP 180
            K    +++ + +SS++L   D++ L++ W VYRSDS+  +  +F++S  D  T  TET 
Sbjct: 121 EKKREKYSILVYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETT 180

Query: 181 FVKTSEGKFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYP 240
           FVK+S  +  + LEFD K +PFYLSF LK   G D    E+ +HR T F +PVGF  G+P
Sbjct: 181 FVKSSLSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHP 240

Query: 241 SPLGLSIS-GDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASF 300
            PLGLS    D S NFS FS ++ +VVLCLY+DST++KP LELDLDPY+NR+G++WHAS 
Sbjct: 241 LPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASV 300

Query: 301 EGASNFVSYGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQ 360
           +   +FV YGY+CK     +    +E   IV+DPYA ++  S+ +          K+LG 
Sbjct: 301 DNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVSQ----------KYLGS 360

Query: 361 ISIVPAFDWDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKN 420
           +S  P+FDW  +V PN+P+EKL VYRLNVK FT  +SS+LP+++AGTFSG+ +K+ HLK 
Sbjct: 361 LSKSPSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKT 420

Query: 421 LGVNAVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEV 480
           LG NAVLLEPIF F E++GPYFPFHFFSP + YGPS +  SA+NSMK MVK+LH+ G+EV
Sbjct: 421 LGTNAVLLEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEV 480

Query: 481 IVEVVYTHTSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEF 540
           ++EVV+THT+  GAL+GIDDSSYY+  R  +L+ +S LNCNYP+VQQL+L+SLRYWVTEF
Sbjct: 481 LLEVVFTHTADSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEF 540

Query: 541 HVDGFCFVNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFP 600
           HVDGFCF+NAS LL+G HGE LSRPP VEAIAFDP+L++TKL+AD WDP E++ KE RFP
Sbjct: 541 HVDGFCFINASSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFP 600

Query: 601 HWKRWAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLP 660
           HWKRWAE+N+++C ++R+F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I+RN GL 
Sbjct: 601 HWKRWAELNTRYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLS 660

Query: 661 LVDLVSFSNSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDE 720
           LVD+VSFS   LASELSWNCGEEG TN   VL++RLKQIRNF+F+ ++SLGVPVLNMGDE
Sbjct: 661 LVDIVSFSGPELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDE 720

Query: 721 CGQSSGGSVAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENID 780
           CG S+ GS     R+ F+W+LL + FGTQ TQFI+F++S R+RR D+FQ R+FLK ENI 
Sbjct: 721 CGISTRGSPLLESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIV 780

Query: 781 WFDNNQSPPQWEDASCKFLAVMLRADKEENESIT---ENPKTRSDIFMAFNASDQSESVA 840
           W+ N+Q+ P+WED + KFLA+ ++++ EE E+ +    N    +D+F+ FNASD  ESV 
Sbjct: 781 WYANDQTTPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVV 840

Query: 841 LPEPSEGTSWFRVVDTALPFPGFFSSDGELV----PMTGSVTYEMQAHSCALFEAKSANA 886
           LP   +G+ W R+VDTALPFPGFFS +GE V    P+   V YEM+ +SC LFE  +  A
Sbjct: 841 LPSLPDGSKWRRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFETINTTA 882

BLAST of MELO3C015861.2 vs. TAIR10
Match: AT4G09020.1 (isoamylase 3)

HSP 1 Score: 377.1 bits (967), Expect = 3.0e-104
Identity = 250/701 (35.66%), Postives = 360/701 (51.36%), Query Frame = 0

Query: 231 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----NDSTSEKPLLELDLDPYINRSG 290
           G  SPLG+S   D  INF++FS  A SV LCL       D T +  ++EL LDP +N++G
Sbjct: 88  GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 291 NIWHASFEGAS-NFVSYGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLG 350
           + WH   E    N V YGY+  G    +     + S +++DPYAK L        D    
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAK-LVKGHSSFGDSSQK 207

Query: 351 FPLKFLGQISIVPA-FDWDGEVR-PNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSG 410
           F  +F G      + FDW  + + PN+P + L +Y +NV+ FT D+SS +   I G++ G
Sbjct: 208 F-AQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267

Query: 411 LTKKLLHLKNLGVNAVLLEPIFQFDEKE--------------GPYFPFHFFSPTNNYGP- 470
             +K+ HL++LG+NAV L P+F+FDE E                Y   +FF+P + Y   
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327

Query: 471 SGASISAINSMKEMVKELHANGVEVIVEVVYTHTS-------VDGALQGIDDSSYYFA-- 530
            G  I A    KEMVK LH+ G+EVI++VVY HT+          + +GID+  YY    
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387

Query: 531 -NRVANLEE-RSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLQGHHGELLSR 590
            N++ N     + LNCN+P+V +L+LDSLR+WVTE+HVDGF F  AS L +   G  LS 
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447

Query: 591 PPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEVNSKFCSDIRDFFRGE- 650
           PP + AIA D +LS+ K++A+ WD   L     +FP+W RWAE N  +  D+R F +G+ 
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWDCGGLY-LVGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507

Query: 651 GLISSLATRLCGSGDVFS-DGRGPAFSFNFIARNVGLPLVDLVSFS-----------NSN 710
           G+  S ATR+ GS D++  + R P    NF+  + G  L DLVS++           N  
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567

Query: 711 LASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSS-GGSVA 770
                SWNCG EG T    +   R +Q++NF   L +S G P++ MGDE G +  G + +
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNS 627

Query: 771 FNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRR-FDLFQSRNFLKGENIDWFDNNQSPP 830
           +    S N    K     +   F  F    + R    + +  NFL    I W ++N    
Sbjct: 628 YGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWHEDN---- 687

Query: 831 QWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWFR 882
            W+++  KFLA  L      ++ I        DI++AFNA D      +P+P  G  WFR
Sbjct: 688 -WDNSESKFLAFTL------HDGIGGR-----DIYVAFNAHDYFVKALIPQPPPGKQWFR 747

BLAST of MELO3C015861.2 vs. TAIR10
Match: AT2G39930.1 (isoamylase 1)

HSP 1 Score: 344.0 bits (881), Expect = 2.8e-94
Identity = 236/692 (34.10%), Postives = 342/692 (49.42%), Query Frame = 0

Query: 231 GYPSPLGLSISGDGSINFSIFSSTAESVVLCL--YNDSTSEKPLLELDLDPYINRSGNIW 290
           G PSP G ++  DG +NFS++S+ + S  +CL   +D    K   E+ LDP  NR+G++W
Sbjct: 72  GLPSPFGPTVRDDG-VNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVW 131

Query: 291 HASFEGASNFVSYGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQ--GLGFP 350
           H    G    + YGY+  G  S E     + S I++DPYAK +      SRD+   LG  
Sbjct: 132 HVFLRGDFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAII-----SRDEFGVLGPD 191

Query: 351 LKFLGQIS-IVPA----FDWDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFS 410
                Q++ +VP     FDW+G++   LP + L +Y ++V+ FT  +SS++  +  GT+ 
Sbjct: 192 DNCWPQMACMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQ 251

Query: 411 GLTKKLLHLKNLGVNAVLLEPIFQFDEKE---------------GPYFPFHFFSPTNNYG 470
           G+ +KL HLK LG+N + L P  +F+E E                 Y    FFSP   Y 
Sbjct: 252 GVAEKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYA 311

Query: 471 PSGASI---SAINSMKEMVKELHANGVEVIVEVVYTHTSVDG------ALQGIDDSSYYF 530
            + ++     AIN  K +VKE H  G+EVI++VV  HT+         + +G+D+S YY 
Sbjct: 312 SASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYM 371

Query: 531 ANRVANLEERS----ALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLQGH---- 590
                     S      NCN+P+V+Q +LD LRYWVTE HVDGF F   S + +      
Sbjct: 372 LAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWD 431

Query: 591 ---------HGELL------SRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHW 650
                     G+LL      S PP ++ I+ DPIL   KL+A+ WD   L  +   FPHW
Sbjct: 432 AANVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLY-QVGMFPHW 491

Query: 651 KRWAEVNSKFCSDIRDFFRG-EGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPL 710
             W+E N KF   +R F +G +G   + A  LCGS +++  GR P  S NFI  + G  L
Sbjct: 492 GIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTL 551

Query: 711 VDLVSFSN-SNLAS----------ELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSL 770
            DLV+++N +NLA+            SWNCGEEG   ++ V   R +Q+RNF   L VS 
Sbjct: 552 ADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQ 611

Query: 771 GVPVLNMGDECGQSSGGS----VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFD 830
           GVP++ MGDE G + GG+       N    F WD  K +  +   +F   L  FR    +
Sbjct: 612 GVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWD-KKEEAHSDFFRFCRILIKFRD-ECE 671

Query: 831 LFQSRNFLKGENIDWFDNNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMA 851
                +F   + + W       P W + S +F+A  L                + +I++A
Sbjct: 672 SLGLNDFPTAKRLQWHGLAPEIPNWSETS-RFVAFSL------------VDSVKKEIYVA 731

BLAST of MELO3C015861.2 vs. TAIR10
Match: AT5G04360.1 (limit dextrinase)

HSP 1 Score: 72.8 bits (177), Expect = 1.2e-12
Identity = 86/364 (23.63%), Postives = 146/364 (40.11%), Query Frame = 0

Query: 232 YPSPLGLSISGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHAS 291
           Y  PLG   + +G ++  +++ TA++V +C+Y +   + P   +++ P +  +  +W  S
Sbjct: 204 YDGPLGAHFTPEG-VSLHLWAPTAQAVSVCIYKNPLDKSP---MEICP-LKEANGVW--S 263

Query: 292 FEGASNFVSYGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLG 351
            EGA ++    Y  K    H +   LE      DPYA+ L+    K+        L  L 
Sbjct: 264 TEGACSWGGCYYVYKVSVYHPSTMKLETC-YANDPYARGLSADGRKTF-------LVNLD 323

Query: 352 QISIVPAFDWD--GEVRPNL-PMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLL 411
              + P   WD   + +P L     + +Y L+V+ F+ +  +  P +  G  +  +K   
Sbjct: 324 SDDLKPE-GWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPENRGGYLAFTSKDSA 383

Query: 412 HLKNL------GVNAVLLEPIFQF----DEKEG--------------------------- 471
            +K+L      G+  + L P FQF    DEKE                            
Sbjct: 384 GVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQARITEIQ 443

Query: 472 -------PYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTHTSVD 531
                   Y P  +  P  +Y         I   ++MV+ L+  G+ V+++VVY H    
Sbjct: 444 NDDGYNWGYNPVLWGVPKGSYASDPTGPCRIIEFRKMVQALNCTGLNVVLDVVYNHLHAS 503

Query: 532 G------ALQGIDDSSYYFANRVANLEERSALN---CNYPIVQQLLLDSLRYWVTEFHVD 540
           G       L  I    Y   N    +E  + +N     + +V +L+ D L  WV  + VD
Sbjct: 504 GPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIRDDLLNWVVNYKVD 551

BLAST of MELO3C015861.2 vs. TAIR10
Match: AT3G20440.2 (Alpha amylase family protein)

HSP 1 Score: 56.6 bits (135), Expect = 8.9e-08
Identity = 41/161 (25.47%), Postives = 79/161 (49.07%), Query Frame = 0

Query: 399 TFSGLTKKLL-HLKNLGVNAVLLEPIFQF-DEKEGPYFPFHFFSPTNNYGPSGASISAIN 458
           TF   TKK+L H+K  G NA+ L  + +  D     Y   +FF+ ++ YG         +
Sbjct: 416 TFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDYFTVGYRVTNFFAASSRYGTP-------D 475

Query: 459 SMKEMVKELHANGVEVIVEVVYTHTSVD-----GALQGIDDSSYYFANRVANLEERSALN 518
             K +V E H  G+ V +++V+++ + D         G +D  +++  R  +    + + 
Sbjct: 476 DFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFDGSNDCYFHYGKRGHHKHWGTRMF 535

Query: 519 CNYPI-VQQLLLDSLRYWVTEFHVDGFCFVNASFLLQGHHG 552
               + V   L+ +L +W+TE+ VDG+ F + + ++  H+G
Sbjct: 536 KYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIYTHNG 569

BLAST of MELO3C015861.2 vs. Swiss-Prot
Match: sp|Q8L735|ISOA2_ARATH (Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2)

HSP 1 Score: 937.6 bits (2422), Expect = 1.0e-271
Identity = 478/900 (53.11%), Postives = 635/900 (70.56%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
           MA +  S+ I   C++N G+T + K   S  L  G+K K + G   E  M          
Sbjct: 1   MAAWSPSVGIGSCCLNN-GITRTWKFP-SARLFTGRKNKIKLG--SETLMFTRKRFMGDL 60

Query: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSK-----EQRKVATYLFRTEFGDLVNVFV 120
           V S+     ++Y  S I   K+   L     S+     + +KV +Y FRT+ G LV V V
Sbjct: 61  VTSA----LQSYQFSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKV 120

Query: 121 GKTGSAFTVNIEISSMQLVSTDET-LLLSWRVYRSDSAP-VTSNFQSSPPDGTTGATETP 180
            K    +++ + +SS++L   D++ L++ W VYRSDS+  +  +F++S  D  T  TET 
Sbjct: 121 EKKREKYSILVYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETT 180

Query: 181 FVKTSEGKFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYP 240
           FVK+S  +  + LEFD K +PFYLSF LK   G D    E+ +HR T F +PVGF  G+P
Sbjct: 181 FVKSSLSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHP 240

Query: 241 SPLGLSIS-GDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASF 300
            PLGLS    D S NFS FS ++ +VVLCLY+DST++KP LELDLDPY+NR+G++WHAS 
Sbjct: 241 LPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASV 300

Query: 301 EGASNFVSYGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQ 360
           +   +FV YGY+CK     +    +E   IV+DPYA ++  S+ +          K+LG 
Sbjct: 301 DNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVSQ----------KYLGS 360

Query: 361 ISIVPAFDWDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKN 420
           +S  P+FDW  +V PN+P+EKL VYRLNVK FT  +SS+LP+++AGTFSG+ +K+ HLK 
Sbjct: 361 LSKSPSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKT 420

Query: 421 LGVNAVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEV 480
           LG NAVLLEPIF F E++GPYFPFHFFSP + YGPS +  SA+NSMK MVK+LH+ G+EV
Sbjct: 421 LGTNAVLLEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEV 480

Query: 481 IVEVVYTHTSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEF 540
           ++EVV+THT+  GAL+GIDDSSYY+  R  +L+ +S LNCNYP+VQQL+L+SLRYWVTEF
Sbjct: 481 LLEVVFTHTADSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEF 540

Query: 541 HVDGFCFVNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFP 600
           HVDGFCF+NAS LL+G HGE LSRPP VEAIAFDP+L++TKL+AD WDP E++ KE RFP
Sbjct: 541 HVDGFCFINASSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFP 600

Query: 601 HWKRWAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLP 660
           HWKRWAE+N+++C ++R+F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I+RN GL 
Sbjct: 601 HWKRWAELNTRYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLS 660

Query: 661 LVDLVSFSNSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDE 720
           LVD+VSFS   LASELSWNCGEEG TN   VL++RLKQIRNF+F+ ++SLGVPVLNMGDE
Sbjct: 661 LVDIVSFSGPELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDE 720

Query: 721 CGQSSGGSVAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENID 780
           CG S+ GS     R+ F+W+LL + FGTQ TQFI+F++S R+RR D+FQ R+FLK ENI 
Sbjct: 721 CGISTRGSPLLESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIV 780

Query: 781 WFDNNQSPPQWEDASCKFLAVMLRADKEENESIT---ENPKTRSDIFMAFNASDQSESVA 840
           W+ N+Q+ P+WED + KFLA+ ++++ EE E+ +    N    +D+F+ FNASD  ESV 
Sbjct: 781 WYANDQTTPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVV 840

Query: 841 LPEPSEGTSWFRVVDTALPFPGFFSSDGELV----PMTGSVTYEMQAHSCALFEAKSANA 886
           LP   +G+ W R+VDTALPFPGFFS +GE V    P+   V YEM+ +SC LFE  +  A
Sbjct: 841 LPSLPDGSKWRRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFETINTTA 882

BLAST of MELO3C015861.2 vs. Swiss-Prot
Match: sp|Q6AU80|ISOA2_ORYSJ (Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=1 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 1.4e-199
Identity = 350/718 (48.75%), Postives = 475/718 (66.16%), Query Frame = 0

Query: 183 SVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPV----GFGRGYPSPLGL 242
           + EL FD    PFYLSF+L      D+  +EIR+HRKTSF VPV                
Sbjct: 105 AAELSFDPTRAPFYLSFLL-----TDASGAEIRTHRKTSFRVPVXXXXXXXXXXXXXXXX 164

Query: 243 SISGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNF 302
                                             LE+DLDPYI+R+GN+WH S      +
Sbjct: 165 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXALEIDLDPYIHRTGNVWHVSLASVDGY 224

Query: 303 VSYGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSR--DQGLGFP-LKFLGQISI 362
           VSY + C G++           R ++DPYAK++   +  +   D+G+  P ++    ++I
Sbjct: 225 VSYAFCCGGIR-----------RPLLDPYAKVIGDFVSSNSVYDEGVTAPSMRCFASLAI 284

Query: 363 VPAFDWDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGV 422
            P+++W  +  P LP+EKL VYR NV  FT D+SS LP D AGTF+GL+ K+ H ++LGV
Sbjct: 285 APSYNWGRDRHPRLPLEKLVVYRANVALFTKDRSSGLPDDAAGTFTGLSAKVEHFRSLGV 344

Query: 423 NAVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVE 482
           NA+LLEP+F F + +GPYFP+HFFSP N Y   G S+SAI SMK+MV+ +H NG+EV++E
Sbjct: 345 NAILLEPVFPFHQVKGPYFPYHFFSPMNLYSSKGLSVSAIKSMKDMVRVMHRNGIEVLLE 404

Query: 483 VVYTHTSVDG------ALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWV 542
           VV+THT+         +++GID+SSYY AN +A   + S LNCN+P+ Q+L+LDSLR+WV
Sbjct: 405 VVFTHTAEGESECQTISMRGIDNSSYYIANGIAGC-KASILNCNHPVTQKLILDSLRHWV 464

Query: 543 TEFHVDGFCFVNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKET 602
            +FHVDGFCF+NA FL++G  GE LSRPP +EAI FDP+LS TK++AD W P ++ + + 
Sbjct: 465 LDFHVDGFCFINAPFLVRGPGGEYLSRPPLLEAITFDPVLSMTKIIADPWSPLDISNVQF 524

Query: 603 RFPHWKRWAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNV 662
            FPHWKRWAEVN++F  D+R F + E LIS LATRLCGSGD+FS  RGPAFSFN ++RN 
Sbjct: 525 PFPHWKRWAEVNTRFSIDVRKFLKREALISDLATRLCGSGDLFST-RGPAFSFNHVSRNS 584

Query: 663 GLPLVDLVSFSNSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNM 722
           GL LVDLVSFSN +L SE SWNCGEEGP+    VL+ RL+QIRNF+F+LFVSLGVPVLNM
Sbjct: 585 GLSLVDLVSFSNDDLLSESSWNCGEEGPSENSAVLQTRLRQIRNFLFILFVSLGVPVLNM 644

Query: 723 GDECGQSSGGSVAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGE 782
           GDECG S+ GSV++ DR   NW  +KT F  + T FI+FL++ RSRR D+FQ R FLK E
Sbjct: 645 GDECGHSAAGSVSYKDRGPLNWRGMKTTFVKEVTGFISFLTALRSRRGDIFQRREFLKLE 704

Query: 783 NIDWFDNNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVA 842
           NI W+ ++   P W+D +  FL + + A+ +E  +       R D+++ FNA+++S S A
Sbjct: 705 NIHWYGSDLCEPGWDDPTSNFLCMHINAEVDEMAA----DSVRGDLYICFNANEESVSAA 764

Query: 843 LPEPSEGTSWFRVVDTALPFPGFFS--SDGELVPMTGSVTYEMQAHSCALFEAKSANA 886
           LP  +EG+ W R+VDT+L FPGFF+  S+ ++  + G  +Y ++AH+C LFE+KSA A
Sbjct: 765 LPALAEGSVWLRLVDTSLAFPGFFATESNPKVQQVPGLSSYHVEAHTCVLFESKSALA 800

BLAST of MELO3C015861.2 vs. Swiss-Prot
Match: sp|Q9M0S5|ISOA3_ARATH (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 377.1 bits (967), Expect = 5.4e-103
Identity = 250/701 (35.66%), Postives = 360/701 (51.36%), Query Frame = 0

Query: 231 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----NDSTSEKPLLELDLDPYINRSG 290
           G  SPLG+S   D  INF++FS  A SV LCL       D T +  ++EL LDP +N++G
Sbjct: 88  GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 291 NIWHASFEGAS-NFVSYGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLG 350
           + WH   E    N V YGY+  G    +     + S +++DPYAK L        D    
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAK-LVKGHSSFGDSSQK 207

Query: 351 FPLKFLGQISIVPA-FDWDGEVR-PNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSG 410
           F  +F G      + FDW  + + PN+P + L +Y +NV+ FT D+SS +   I G++ G
Sbjct: 208 F-AQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267

Query: 411 LTKKLLHLKNLGVNAVLLEPIFQFDEKE--------------GPYFPFHFFSPTNNYGP- 470
             +K+ HL++LG+NAV L P+F+FDE E                Y   +FF+P + Y   
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327

Query: 471 SGASISAINSMKEMVKELHANGVEVIVEVVYTHTS-------VDGALQGIDDSSYYFA-- 530
            G  I A    KEMVK LH+ G+EVI++VVY HT+          + +GID+  YY    
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387

Query: 531 -NRVANLEE-RSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLQGHHGELLSR 590
            N++ N     + LNCN+P+V +L+LDSLR+WVTE+HVDGF F  AS L +   G  LS 
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447

Query: 591 PPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEVNSKFCSDIRDFFRGE- 650
           PP + AIA D +LS+ K++A+ WD   L     +FP+W RWAE N  +  D+R F +G+ 
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWDCGGLY-LVGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507

Query: 651 GLISSLATRLCGSGDVFS-DGRGPAFSFNFIARNVGLPLVDLVSFS-----------NSN 710
           G+  S ATR+ GS D++  + R P    NF+  + G  L DLVS++           N  
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567

Query: 711 LASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSS-GGSVA 770
                SWNCG EG T    +   R +Q++NF   L +S G P++ MGDE G +  G + +
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNS 627

Query: 771 FNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRR-FDLFQSRNFLKGENIDWFDNNQSPP 830
           +    S N    K     +   F  F    + R    + +  NFL    I W ++N    
Sbjct: 628 YGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWHEDN---- 687

Query: 831 QWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWFR 882
            W+++  KFLA  L      ++ I        DI++AFNA D      +P+P  G  WFR
Sbjct: 688 -WDNSESKFLAFTL------HDGIGGR-----DIYVAFNAHDYFVKALIPQPPPGKQWFR 747

BLAST of MELO3C015861.2 vs. Swiss-Prot
Match: sp|B9G434|ISOA3_ORYSJ (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.7e-101
Identity = 250/730 (34.25%), Postives = 371/730 (50.82%), Query Frame = 0

Query: 212 SEIRSHRKTSFSVPVGFGRGYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----NDS 271
           +E R    +   +P  +  G   PLG+S   +G +NF++FS  A SV+LCL       + 
Sbjct: 84  TEERGCTMSDTEMPFKYSSGKAFPLGVS-QVEGGLNFALFSQHASSVILCLKLPGRGTED 143

Query: 272 TSEKPLLELDLDPYINRSGNIWHASFEG-ASNFVSYGYQCKGLKSHENQDGLEVSRIVVD 331
                ++E  LD   N++G+IWH   EG  ++ V YGY+  G +  +     + S +++D
Sbjct: 144 EKGADVVEFVLDQQKNKTGDIWHVIVEGLPASGVLYGYRVGGPQGWDQGHRFDSSTVLLD 203

Query: 332 PYAKILAP----SIPKSRDQGLGFPLKFLGQISIVPA-FDWDGEVR-PNLPMEKLFVYRL 391
           PYAK+++      + + +        +  G      + FDW  + R PNLP   L +Y +
Sbjct: 204 PYAKLVSGRKYFGVAEEKSS------QHFGTYDFDSSPFDWGDDYRLPNLPEADLVIYEM 263

Query: 392 NVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLLEPIFQFDEKEGPYFP---- 451
           NV+ FT D+SS L +   G++ GL  K+ HL  LGVNAV L P+F++DE E   +P    
Sbjct: 264 NVRAFTADESSGLDSTSRGSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNPRD 323

Query: 452 ----------FHFFSPTNNYGPSGAS-ISAINSMKEMVKELHANGVEVIVEVVYTHTS-V 511
                      +FF+P + Y  +G   ++A   +K+MVKELH  G+EVI++VVY HT+  
Sbjct: 324 HMVNTWGYSTINFFAPMSRYASAGGGPVAASKELKQMVKELHKAGIEVILDVVYNHTNEA 383

Query: 512 DGA------LQGIDDSSYYFANRVANLEE------RSALNCNYPIVQQLLLDSLRYWVTE 571
           D A       +GID+  YY  +   N E        + LNCN+P+V++L+LDSLR+WV E
Sbjct: 384 DDAHPYMTSFRGIDNKVYYMLDLNKNAELLNFSGCGNTLNCNHPVVKELILDSLRHWVEE 443

Query: 572 FHVDGFCFVNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRF 631
           +H+DGF F  AS L +G  G  L  PP ++ IA D +LS+ K++A+ WD   L     RF
Sbjct: 444 YHIDGFRFDLASVLCRGPDGCPLDAPPLIKEIAKDAVLSRCKIIAEPWDCGGLY-LVGRF 503

Query: 632 PHWKRWAEVNSKFCSDIRDFFRGE-GLISSLATRLCGSGDVFS-DGRGPAFSFNFIARNV 691
           P+W RWAE N K+  D+R F +G+ G+    ATR+ GS D++  + R P    NF+  + 
Sbjct: 504 PNWDRWAEWNGKYRDDLRRFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNFVIAHD 563

Query: 692 GLPLVDLVSFSNSN-----------LASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVL 751
           G  L DLVS++  +                SWNCG EG TN L VL  R +Q++NF   L
Sbjct: 564 GFTLCDLVSYNLKHNDANGEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKNFHVAL 623

Query: 752 FVSLGVPVLNMGDECG-------QSSGGSVAFNDRRSFNWDLLKTDFGTQTTQFIAFLSS 811
            +S G P++ MGDE G        S G     N   +F W+ L+        +F + +  
Sbjct: 624 MISQGTPMMLMGDEYGHTRYGNNNSYGHDTCIN---NFQWEQLEQRRDGH-FRFFSEMIK 683

Query: 812 FRSRRFDLFQSRNFLKGENIDWFDNNQSPPQWEDASCKFLAVMLRADKEENESITENPKT 871
           FR     L + R FL   ++ W ++      WE+   KFLA  +            +  +
Sbjct: 684 FRHSNPILRRDR-FLNKNDVTWHED-----CWENQESKFLAFTV-----------HDHNS 743

Query: 872 RSDIFMAFNASDQSESVALPEPSEGTSWFRVVDTALPFPGFFSSDGELVPMTGSVTYEMQ 882
             DI++AFNA D      +P P     W RVVDT L  P     +G  VP TG   Y + 
Sbjct: 744 GGDIYLAFNAHDYFVDAVIPPPPHHKCWNRVVDTNLESPNDIVPEG--VPFTGP-KYRIA 781

BLAST of MELO3C015861.2 vs. Swiss-Prot
Match: sp|D0TZF0|ISOA1_ORYSJ (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 8.3e-96
Identity = 232/693 (33.48%), Postives = 347/693 (50.07%), Query Frame = 0

Query: 231 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY--NDSTSEKPLLELDLDPYINRSGNIW 290
           G P+PLG + + DG +NF+++S+ A +  LCL+  +D  +++   E+ LDP  NR+GN+W
Sbjct: 99  GMPAPLG-ATALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLDPLFNRTGNVW 158

Query: 291 HASFEGASNFVSYGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFP-- 350
           H   EG  + + YGY+  G+ +       +VS +VVDPYAK    ++    + G+  P  
Sbjct: 159 HVFIEGELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAK----AVISRGEYGVPGPGG 218

Query: 351 ---LKFLGQISI-VPAFDWDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSG 410
               +  G I +    FDW G++    P + L +Y ++++ FT   SS +  +  GT+ G
Sbjct: 219 DCWPQMAGMIPLPYSTFDWQGDLPLRYPQKDLVIYEMHLRGFTKHSSSNV--EHPGTYIG 278

Query: 411 LTKKLLHLKNLGVNAVLLEPIFQFDEKE----------GPYFPFHFFSPTNNYGPSG--- 470
              KL +LK LGVN V L P  +F+E E            Y   +FFSP   Y   G   
Sbjct: 279 AISKLDYLKELGVNCVELMPCHEFNELEYFSCSSKMNFWGYSTINFFSPMIRYSSGGIRN 338

Query: 471 ASISAINSMKEMVKELHANGVEVIVEVVYTHTSVDG------ALQGIDDSSYYFANRVAN 530
               AIN  K  V+E H  G+EVI++VV+ HT+         + +GID+S+YY       
Sbjct: 339 CGRDAINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSTYYMLAPKGE 398

Query: 531 LEERS----ALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLQG----------- 590
               S      NCN+P+V++ ++D LRYWVTE HVDGF F  AS + +G           
Sbjct: 399 FYNYSGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYG 458

Query: 591 --------HHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 650
                     G  L+ PP ++ I+ DPIL   KL+A+ WD   L  +  +FPHWK W+E 
Sbjct: 459 SPVEGDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWDAGGLY-QVGQFPHWKIWSEW 518

Query: 651 NSKFCSDIRDFFRG-EGLISSLATRLCGSGDVF-SDGRGPAFSFNFIARNVGLPLVDLVS 710
           N K+   +R F +G +G     A  LCGS  ++ + GR P  S NF+  + G  L DLV+
Sbjct: 519 NGKYRDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVT 578

Query: 711 FSNSNLAS-----------ELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVL 770
           ++    +S            LSWNCGEEG    L V   R +Q+RNF   L VS GVP+ 
Sbjct: 579 YNKKYNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMF 638

Query: 771 NMGDECGQSSGG--SVAFNDR--RSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSR 830
            MGDE G + GG  +   +D     F WD  K +  +   +F + ++ FR ++ +     
Sbjct: 639 YMGDEYGHTKGGNNNTYCHDHYVNYFRWD--KKEESSDLQRFCSLMTKFR-KQCESLGLA 698

Query: 831 NFLKGENIDWFDNNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASD 857
           +F   + + W  +    P W + S +F+A             +   +T+ +I++AFNAS 
Sbjct: 699 DFPTAQRLHWHGHQPGKPDWSETS-RFVA------------FSTKDETKGEIYVAFNASH 758

BLAST of MELO3C015861.2 vs. TrEMBL
Match: tr|A0A1S3BRF8|A0A1S3BRF8_CUCME (isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1)

HSP 1 Score: 1786.2 bits (4625), Expect = 0.0e+00
Identity = 885/885 (100.00%), Postives = 885/885 (100.00%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
           MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
           VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
           AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300
           SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
           YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
           WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
           NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
           VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
           PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSANA 886
           RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSANA
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSANA 885

BLAST of MELO3C015861.2 vs. TrEMBL
Match: tr|A0A0A0K551|A0A0A0K551_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 SV=1)

HSP 1 Score: 1686.0 bits (4365), Expect = 0.0e+00
Identity = 830/884 (93.89%), Postives = 852/884 (96.38%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
           MATFLSSLVIQPFCV+NCGVTES KLA SDHLTYGQKTKYQ GKMDEARMLAHGE+KVGA
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
           VKSSHRNLSK Y  SGI VGKSGQRLGIGGKSKEQR+VATYLFRTEFGDLVNVFVGK GS
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
            FTVNIE+ SMQLVS DE LLLSW VYRSDSA VT NF+SSPPD TTGATETPFVKTSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLK+PMGVDSGSSEIRSH+KTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300
           SGDGS+NFSIFSS+AES+VLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGAS FVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
           YGYQCKG KSHENQDGLEVSRIVVDPYAKILAPSIPKS  QGLG P KFLGQIS VP FD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
           WDGEV PNLPMEKLFVYRLNV+RFT+DKSSQLPADIAGTFSGLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVI+EVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480

Query: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           TS +GALQGIDDSSYYF NRVANLEE+SALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
           NASFLL+GHHGELLSRPPFVEAIAFDP+LSKTKLVADFWDPQEL SKETRFPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPT+ LKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
           VAFND+RSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQ+RNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
           PQWEDASCKFLAVMLRADKEENESITENPKTRS+IFM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 885
           RVVDTALPFPGFFSSDGELVPMTGSVTYE+QAHSCALFEAKSAN
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 884

BLAST of MELO3C015861.2 vs. TrEMBL
Match: tr|A0A2N9GWM1|A0A2N9GWM1_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS31596 PE=4 SV=1)

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 553/886 (62.42%), Postives = 679/886 (76.64%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
           MAT   SL IQP C   CG + S KL+ + H       +Y   KMD  R L  GE+    
Sbjct: 1   MATLPPSLAIQP-CNLKCGASGSSKLSPACHYICRNHIRYGFEKMDLERKLICGEAAQNV 60

Query: 61  VKSSHRNL-SKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTG 120
           VKS  ++L SK + TS +LV +  Q++    +++   KV+TYLF TE G  V VFV K  
Sbjct: 61  VKSPCQHLNSKVFATSRVLVEEIEQKVSTATEAEGLEKVSTYLFWTEVGGQVKVFVRKRS 120

Query: 121 SAFTVNIEISSMQLVSTDETLLLSWRVYRSDSAP-VTSNFQSSPPDGTTGATETPFVKTS 180
             + VNIE+SS+ L S+++ L LSW  YR+DS+  +  + QSS PD     +ETPF++TS
Sbjct: 121 VKYVVNIEVSSLPLHSSEDRLTLSWGRYRADSSSFMPLDVQSSEPDTRISTSETPFIQTS 180

Query: 181 EGKFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGL 240
            G+F++ELEF+AK  PFYLSF+LK          EIRSHRKT+F VPVG G GYP+PLGL
Sbjct: 181 AGRFALELEFEAKQFPFYLSFLLKLQSDSGLSGLEIRSHRKTNFCVPVGLGTGYPAPLGL 240

Query: 241 SISGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNF 300
           S S DGS+NF+IFS  AE V+LCLY+D+T+++P LELDLDPY+NRSG+IWHASFE A  F
Sbjct: 241 SFSPDGSMNFAIFSRYAEGVILCLYDDTTADEPALELDLDPYVNRSGDIWHASFESAWTF 300

Query: 301 VSYGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPA 360
           VSYGY+CKG     + + L+  +I++DPYAKI+  S P S   G     K+LGQ+   PA
Sbjct: 301 VSYGYRCKGSLLQRSNNKLDAGQIILDPYAKIIGNSSPSS--HGSKLLQKYLGQLCKEPA 360

Query: 361 FDWDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAV 420
           FDW  +  P LPMEKL VYRLNV+ FT  KSSQLP D+AGTFSGLT+KL H K+LG+NAV
Sbjct: 361 FDWGDDTHPYLPMEKLLVYRLNVRHFTEHKSSQLPTDVAGTFSGLTEKLQHFKDLGMNAV 420

Query: 421 LLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVY 480
           LLEPIF FDEK GPYFPFHFFSP N YGPSG S+SAINSMKEMVK++HANG+EV++EVV+
Sbjct: 421 LLEPIFSFDEKNGPYFPFHFFSPMNLYGPSGGSVSAINSMKEMVKKMHANGIEVLLEVVF 480

Query: 481 THTSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFC 540
           THT+  G+LQGIDD SYY+AN+V +LE   ALNCNYPIVQQL+LDSLR+WVTEFHVDGFC
Sbjct: 481 THTAAAGSLQGIDDLSYYYANKVGDLEAPKALNCNYPIVQQLILDSLRHWVTEFHVDGFC 540

Query: 541 FVNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWA 600
           F+NAS LL+G HGE LSRPP +EAIAFDP+LSKTK++AD WDP +++ KE RFPHWKRWA
Sbjct: 541 FINASSLLRGFHGEYLSRPPLIEAIAFDPLLSKTKIIADCWDPHDMLPKENRFPHWKRWA 600

Query: 601 EVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVS 660
           E+++KFC+D+R+F+RGEG++S LATRLCGSGD+FSDGRGPAFSFNFIARN GL LVDLVS
Sbjct: 601 EIDTKFCNDVRNFWRGEGVLSDLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDLVS 660

Query: 661 FSNSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSG 720
           FS+  LAS+LSWNCGEEGPT+   VLE+RLKQIRNF+FVL++SLGVP+LNMGDECGQSSG
Sbjct: 661 FSSDALASQLSWNCGEEGPTDNTTVLERRLKQIRNFLFVLYISLGVPILNMGDECGQSSG 720

Query: 721 GSVAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQ 780
           GS A+ DR+ F+W+ LKT FG QT+QFI+FLS FR RR DL Q RNFLK ENIDW   +Q
Sbjct: 721 GSPAYTDRKPFDWNALKTGFGIQTSQFISFLSLFRMRRSDLLQKRNFLKEENIDWHGRDQ 780

Query: 781 SPPQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTS 840
            PP+WED S +FLA+ LRADK E+   TE+   R D+F+AFNA+D SESV LP P EG S
Sbjct: 781 FPPKWEDPSRRFLAMTLRADKVESPLSTESSHLRGDLFIAFNAADHSESVILPTPPEGMS 840

Query: 841 WFRVVDTALPFPGFFSSDGELVPMTGS--VTYEMQAHSCALFEAKS 883
           W  +VDTALPFPGFFS++GE +P   +  V Y+M++HSCALFEA S
Sbjct: 841 WLCMVDTALPFPGFFSTNGEPLPKQKAELVAYKMKSHSCALFEASS 883

BLAST of MELO3C015861.2 vs. TrEMBL
Match: tr|A0A2P4IIC1|A0A2P4IIC1_QUESU (Isoamylase 2, chloroplastic OS=Quercus suber OX=58331 GN=CFP56_26168 PE=4 SV=1)

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 551/889 (61.98%), Postives = 676/889 (76.04%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
           MAT   SL IQ   +  CG+++  K + + H  Y    +Y   KMD  R L   E     
Sbjct: 1   MATLPPSLAIQSGYL-KCGISDVSKSSPACHYIYRNHIRYGFEKMDLERRLICDEVSQNV 60

Query: 61  VKSSHRNL-SKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTG 120
           VKSS RNL S+ + TS +LV +  Q++    ++  + K  TYLF TE G  V VFV KT 
Sbjct: 61  VKSSCRNLNSRVFATSRVLVEEIEQKVSTITEADNREKALTYLFWTEVGGQVKVFVRKTS 120

Query: 121 SAFTVNIEISSMQLVSTDETLLLSWRVYRSDSAP-VTSNFQSSPPDGTTGATETPFVKTS 180
             + VNIE+SS+QL S+++ L+ SW  YR+DS+  +  + QSS PD  T  +ETPF++TS
Sbjct: 121 VKYAVNIEVSSLQLQSSEDRLISSWGRYRADSSSFIPLDVQSSVPDSRTPTSETPFIQTS 180

Query: 181 EGKFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGL 240
            G+F++ELEF+AK  PFYLSF+LK P    S  SEIRSHRKT F VPVGF +GYP+PLGL
Sbjct: 181 AGRFALELEFEAKQIPFYLSFILKLPSDSGSRGSEIRSHRKTKFCVPVGFRKGYPAPLGL 240

Query: 241 SISGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNF 300
           S S DGS+NF+IFS   E V+LC Y+D+ +E+P LELDLDPYINRSG+IWHASFE A  F
Sbjct: 241 SFSPDGSMNFAIFSRNVEGVILCFYDDAKAEEPALELDLDPYINRSGDIWHASFESAWTF 300

Query: 301 VSYGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPA 360
           VSYGYQCKG     N +  +  +I++DPYAKI+  SIP S         K+LG++   P 
Sbjct: 301 VSYGYQCKGSLLQRNNNKFDAGQIILDPYAKIIVNSIPSSDGS-----QKYLGRLCKEPG 360

Query: 361 FDWDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAV 420
           FDW  +  P LPMEKL VYRLNV+ FT  KSSQL  D+AGTF GLT+KL H K+LG+NAV
Sbjct: 361 FDWGDDTHPYLPMEKLVVYRLNVRHFTEHKSSQLATDVAGTFIGLTEKLQHFKDLGMNAV 420

Query: 421 LLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVY 480
           LLEPIF FDEK GPYFP HFFSPTN YGPSG S+SAINSMKEMVK+LHANG+EV++EVV+
Sbjct: 421 LLEPIFSFDEKNGPYFPCHFFSPTNLYGPSGGSVSAINSMKEMVKKLHANGIEVLLEVVF 480

Query: 481 THTSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFC 540
           THT+V G+LQGIDD SYY  N   +LE  + LNCNYPIVQQL+LDSL+YWVTEFH+DGFC
Sbjct: 481 THTAVAGSLQGIDDISYYHVNEGEDLEATNYLNCNYPIVQQLILDSLQYWVTEFHIDGFC 540

Query: 541 FVNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWA 600
           F+NAS LL+G HGE LSRPP VEAI+FDP+LSKTK++AD WDP +++ KETRFPHWKRWA
Sbjct: 541 FINASSLLRGFHGEYLSRPPLVEAISFDPLLSKTKIIADCWDPHDMLPKETRFPHWKRWA 600

Query: 601 EVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVS 660
           E+++KFC+D+R+F+RGEGL+S LATRLCGSGD+FSDGRGPAFSFNFIARN GL LVDLVS
Sbjct: 601 EIDTKFCNDVRNFWRGEGLLSDLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDLVS 660

Query: 661 FSNSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSG 720
           FSN  LAS+LSWNCGEEGPT+   VLE+RLKQIRNF+FVL++SLGVP+LNMGDECGQS G
Sbjct: 661 FSNDALASQLSWNCGEEGPTDNTTVLERRLKQIRNFLFVLYISLGVPILNMGDECGQSCG 720

Query: 721 GSVAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQ 780
           GS A++DR+  +W+ LKT F  QT+QFI+FLSS R RR DL Q RNFLK ENI+W  ++Q
Sbjct: 721 GSPAYSDRKPLDWNTLKTGFSIQTSQFISFLSSLRMRRSDLLQKRNFLKEENIEWHGSDQ 780

Query: 781 SPPQWEDASCKFLAVMLRADKEENESITENPK--TRSDIFMAFNASDQSESVALPEPSEG 840
           SPP+WED SC+FLA+ LRAD+ E+   +E+     R D+F+AFNA+D SESV LP+P EG
Sbjct: 781 SPPKWEDPSCRFLAMTLRADEVESSLNSESSSHVRRGDLFIAFNAADLSESVILPQPPEG 840

Query: 841 TSWFRVVDTALPFPGFFSSDGELVP--MTGSVTYEMQAHSCALFEAKSA 884
            SW  +VDTALPFPGFFS+ GE +P  M  SV YEM++HSCALFEA S+
Sbjct: 841 MSWRCLVDTALPFPGFFSTSGETLPEQMAESVAYEMKSHSCALFEASSS 883

BLAST of MELO3C015861.2 vs. TrEMBL
Match: tr|M5VNY8|M5VNY8_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_8G244800 PE=4 SV=1)

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 549/891 (61.62%), Postives = 681/891 (76.43%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
           MAT   S+ IQ  C  NCG+TES KL  ++   +  K  +   K+D  R L   E     
Sbjct: 1   MATLPLSIAIQA-CCFNCGITESSKLTATNGFRHRDKAMHGFVKLDAERKLVFRE----V 60

Query: 61  VKSSHRNLS-----KAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFV 120
           V++ + NL      K Y  S + V    QR   G +++E  KV+TYLFRTE GD+VNVFV
Sbjct: 61  VQNINENLPRDHNLKVYAKSQVSVEPMEQRFSTGTETEEIDKVSTYLFRTEIGDVVNVFV 120

Query: 121 GKTGSAFTVNIEISSMQLVSTDETLLLSWRVYRSDSAP-VTSNFQSSPPDGTTGATETPF 180
            K  + +TVNIE+ S  L S D  L+L W +YR+DS+  V  +F+SS P+ TT   ETP 
Sbjct: 121 RKRNAKYTVNIEVPSSHLSSNDRRLVLRWGMYRADSSCFVPLDFKSSTPNDTTTTLETPL 180

Query: 181 VKTSEGKFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPS 240
           ++TS G+F++ELEF+AK  PFY SF+L  P   +    EIRSHRKT+F VPVGFGRGYP 
Sbjct: 181 IQTSSGRFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPG 240

Query: 241 PLGLSISGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEG 300
           PLGL+ S DGS+NF+IFS  AESV LCLY+++T+EKP LELDLDPY+NRSG+IWH SF  
Sbjct: 241 PLGLTFSNDGSMNFAIFSRNAESVALCLYDNTTAEKPALELDLDPYVNRSGDIWHVSFGS 300

Query: 301 ASNFVSYGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQIS 360
           A +FVSYGY+ KG     N++  +   +++DPYAK++A SIP +   G    LK+LG++ 
Sbjct: 301 AWSFVSYGYKFKGNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTG----LKYLGRLC 360

Query: 361 IVPAFDWDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLG 420
             PAFDW G+VRP+L MEKL VYRLNV RFT  KSSQLP +I G+FSGLT+KL H K+LG
Sbjct: 361 EEPAFDWAGDVRPDLSMEKLVVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDLG 420

Query: 421 VNAVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIV 480
           VNAVLLEPIF FDE++GPYFP HFFSP + +GPS   +SA+NSMKEMV++ HANG+EV++
Sbjct: 421 VNAVLLEPIFPFDEQKGPYFPHHFFSPMDCFGPSRGPVSAVNSMKEMVRKFHANGIEVLL 480

Query: 481 EVVYTHTSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHV 540
           EVV+THT+   ALQGID SSYY  N V +LE R+ALNCNYPIVQQL+LDSLRYWVTEFHV
Sbjct: 481 EVVFTHTAEGEALQGIDISSYYHVNEVEDLEARNALNCNYPIVQQLVLDSLRYWVTEFHV 540

Query: 541 DGFCFVNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHW 600
           DGF F+NAS LL+G +GE LSRPP VEAIAFDP+LSKTK++AD WDP  +  KET FPHW
Sbjct: 541 DGFFFINASSLLRGFNGEYLSRPPLVEAIAFDPLLSKTKIIADCWDPHGMAPKETHFPHW 600

Query: 601 KRWAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLV 660
           +RWAEVN+KFC+D+R+F RGEGL+S LATRLCG+GD+FSDGRGPAF+FNFI+RN GLPLV
Sbjct: 601 RRWAEVNTKFCNDVRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFAFNFISRNSGLPLV 660

Query: 661 DLVSFSNSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECG 720
           DLVSFS   LASELSWNCGEEGPTN   VLE+RLKQIRNF+F+LFVSLGVPVLNMGDECG
Sbjct: 661 DLVSFSGVELASELSWNCGEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECG 720

Query: 721 QSSGGSVAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWF 780
           QS+GGS A++DR++F+W+ L+T F TQTTQFIAFLSSFR RR DL Q RNFLK ENI W+
Sbjct: 721 QSTGGSPAYSDRKAFDWNALETGFATQTTQFIAFLSSFRKRRSDLLQKRNFLKEENIGWY 780

Query: 781 DNNQSPPQWEDASCKFLAVMLRADKEE-NESITENPKTRSDIFMAFNASDQSESVALPEP 840
           +++Q+PP+WED S KFLA+ L+AD++E N+   E+  +  D+F+AF+A+D SE+V LP P
Sbjct: 781 ESDQTPPRWEDPSRKFLAMRLKADEDEVNQPGDESSHSWGDLFVAFSAADHSETVVLPPP 840

Query: 841 SEGTSWFRVVDTALPFPGFFSSDGELV--PMTGSVTYEMQAHSCALFEAKS 883
            EG  W R+VDTALPFPGFFS+DGE V   + G   YEM++HSCALFEA+S
Sbjct: 841 LEGMGWRRLVDTALPFPGFFSTDGEPVVEQIVGLFAYEMKSHSCALFEARS 882