CSPI07G16810 (gene) Wild cucumber (PI 183967)

BLAST of CSPI07G16810 vs. Swiss-Prot
Match: ISOA2_ARATH (Isoamylase 2, chloroplastic OS=Arabidopsis thaliana GN=ISA2 PE=1 SV=2)

HSP 1 Score: 943.7 bits (2438), Expect = 1.4e-273
Identity = 476/881 (54.03%), Postives = 629/881 (71.40%), Query Frame = 1

Query: 14  CVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGAVKSSHRNLSKTYA 73
           C  N G+T + K  ++   T G+K K + G   E  M          V S+     ++Y 
Sbjct: 13  CCLNNGITRTWKFPSARLFT-GRKNKIKLGS--ETLMFTRKRFMGDLVTSA----LQSYQ 72

Query: 74  KSGISVGKSGQRLGIGGKSK-----EQRRVATYLFRTEFGDLVNVFVGKKGSTFTVNIEV 133
            S I   K+   L     S+     + ++V +Y FRT+ G LV V V KK   +++ + V
Sbjct: 73  FSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYV 132

Query: 134 PSMQLVSIDEA-LLLSWGVYRSDSALVTP-NFQSSPPDETTGATETPFVKTSEGKFSVEL 193
            S++L   D++ L++ WGVYRSDS+   P +F++S  D  T  TET FVK+S  +  + L
Sbjct: 133 SSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGL 192

Query: 194 EFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSIS-GDGS 253
           EFD K +PFYLSF LK   G D    E+ +H+ T F +PVGF  G+P PLGLS    D S
Sbjct: 193 EFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDS 252

Query: 254 INFSIFSSSAENLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVSYGYQC 313
            NFS FS S+ N+VLCLY+DST++KP LELDLDPY+NR+G++WHAS +    FV YGY+C
Sbjct: 253 WNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRC 312

Query: 314 KGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFDWDGEV 373
           K +   +    +E   IV+DPYA ++  S+ +          K+LG +SK P+FDW  +V
Sbjct: 313 KETAHSKEDVDVEGEPIVLDPYATVVGKSVSQ----------KYLGSLSKSPSFDWGEDV 372

Query: 374 RPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQ 433
            PN+P+EKL VYRLNV+ FT  +SS+LP+++AGTFSG+ +K+ H K LG NAVLLEPIF 
Sbjct: 373 SPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFS 432

Query: 434 FDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTHTSGNG 493
           F E++GPYFPFHFFSP + YGPS +  SA+NSMK MVK+LH+ G+EV+LEVV+THT+ +G
Sbjct: 433 FSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG 492

Query: 494 ALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFL 553
           AL+GIDDSSYY+  R  +L+ KS LNCNYP+VQQL+L+SLRYWVTEFHVDGFCF+NAS L
Sbjct: 493 ALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSL 552

Query: 554 LRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFC 613
           LRG HGE LSRPP VEAIAFDPLL++TKL+AD WDP E+  KE RFPHWKRWAE+N+++C
Sbjct: 553 LRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYC 612

Query: 614 SDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSNSNLA 673
            ++R+F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I+RN GL LVD+VSFS   LA
Sbjct: 613 RNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELA 672

Query: 674 SELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFND 733
           SELSWNCGEEG T+   VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS     
Sbjct: 673 SELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLES 732

Query: 734 KRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSPPQWED 793
           ++ F+W+LL + FGTQ TQFI+F++S R+RR D+FQ R+FLK ENI W+ N+Q+ P+WED
Sbjct: 733 RKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWED 792

Query: 794 ASCKFLAVMLRADKEENE--SITENPKTRSN-IFMVFNASDQSESVALPEPLEGTSWFRV 853
            + KFLA+ ++++ EE E  S+ E  + +SN +F+ FNASD  ESV LP   +G+ W R+
Sbjct: 793 PASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRL 852

Query: 854 VDTALPFPGFFSSDGELV----PMTGSVTYEIQAHSCALFE 880
           VDTALPFPGFFS +GE V    P+   V YE++ +SC LFE
Sbjct: 853 VDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of CSPI07G16810 vs. Swiss-Prot
Match: ISOA3_ARATH (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana GN=ISA3 PE=2 SV=2)

HSP 1 Score: 373.6 bits (958), Expect = 5.9e-102
Identity = 246/701 (35.09%), Postives = 362/701 (51.64%), Query Frame = 1

Query: 231 GYPSPLGLSISGDGSINFSIFSSSAENLVLCLY-----NDSTSEKPLLELDLDPYINRSG 290
           G  SPLG+S   D  INF++FS +A ++ LCL       D T +  ++EL LDP +N++G
Sbjct: 88  GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 291 NIWHASFEGAS-KFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLG 350
           + WH   E      V YGY+  G    +     + S +++DPYAK++      S G    
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGH--SSFGDSSQ 207

Query: 351 LPSKFLGQIS-KVPTFDWDGEVR-PNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSG 410
             ++F G    +   FDW  + + PN+P + L +Y +NV  FT D+SS +   I G++ G
Sbjct: 208 KFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267

Query: 411 LTKKLLHFKNLGVNAVLLEPIFQFDEKE-----GP---------YFPFHFFSPTNNYGPS 470
             +K+ H ++LG+NAV L P+F+FDE E      P         Y   +FF+P + Y   
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327

Query: 471 -GASISAINSMKEMVKELHANGVEVILEVVYTHTSG-------NGALQGIDDSSYYF--- 530
            G  I A    KEMVK LH+ G+EVIL+VVY HT+          + +GID+  YY    
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387

Query: 531 TNRVANLEE-KSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLRGHHGELLSR 590
            N++ N     + LNCN+P+V +L+LDSLR+WVTE+HVDGF F  AS L R   G  LS 
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447

Query: 591 PPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFCSDIRDFFRGE- 650
           PP + AIA D +LS+ K++A+ WD   L     +FP+W RWAE N  +  D+R F +G+ 
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWDCGGL-YLVGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507

Query: 651 GLISSLATRLCGSGDVFS-DGRGPAFSFNFIARNVGLPLVDLVSFS-----------NSN 710
           G+  S ATR+ GS D++  + R P    NF+  + G  L DLVS++           N  
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567

Query: 711 LASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSS-GGSVA 770
                SWNCG EG T +  +   R +Q++NF   L +S G P++ MGDE G +  G + +
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNS 627

Query: 771 FNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRR-FDLFQNRNFLKGENIDWFDNNQSPP 830
           +    S N    K     +   F  F    + R    + ++ NFL    I W ++N    
Sbjct: 628 YGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWHEDN---- 687

Query: 831 QWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWFR 882
            W+++  KFLA  L      ++ I        +I++ FNA D      +P+P  G  WFR
Sbjct: 688 -WDNSESKFLAFTL------HDGIGGR-----DIYVAFNAHDYFVKALIPQPPPGKQWFR 747

BLAST of CSPI07G16810 vs. Swiss-Prot
Match: ISOA1_ARATH (Isoamylase 1, chloroplastic OS=Arabidopsis thaliana GN=ISA1 PE=1 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 3.4e-94
Identity = 239/690 (34.64%), Postives = 344/690 (49.86%), Query Frame = 1

Query: 231 GYPSPLGLSISGDGSINFSIFSSSAENLVLCLYN--DSTSEKPLLELDLDPYINRSGNIW 290
           G PSP G ++  DG +NFS++S+++ +  +CL +  D    K   E+ LDP  NR+G++W
Sbjct: 72  GLPSPFGPTVRDDG-VNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVW 131

Query: 291 HASFEGASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSK 350
           H    G  K + YGY+  G  S E     + S I++DPYAK +   I +     LG    
Sbjct: 132 HVFLRGDFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAI---ISRDEFGVLGPDDN 191

Query: 351 FLGQIS-KVPT----FDWDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGL 410
              Q++  VPT    FDW+G++   LP + L +Y ++V  FT  +SS++  +  GT+ G+
Sbjct: 192 CWPQMACMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQGV 251

Query: 411 TKKLLHFKNLGVNAVLLEPIFQFDEKE---------------GPYFPFHFFSPTNNYGPS 470
            +KL H K LG+N + L P  +F+E E                 Y    FFSP   Y  +
Sbjct: 252 AEKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASA 311

Query: 471 GASI---SAINSMKEMVKELHANGVEVILEVVYTHTS-GNG-----ALQGIDDSSYYFTN 530
            ++     AIN  K +VKE H  G+EVI++VV  HT+ GN      + +G+D+S YY   
Sbjct: 312 SSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLA 371

Query: 531 RVANLEEKSA----LNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLRGH------ 590
                   S      NCN+P+V+Q +LD LRYWVTE HVDGF F   S + R        
Sbjct: 372 PKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAA 431

Query: 591 -------HGELL------SRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKR 650
                   G+LL      S PP ++ I+ DP+L   KL+A+ WD   L  +   FPHW  
Sbjct: 432 NVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLY-QVGMFPHWGI 491

Query: 651 WAEVNSKFCSDIRDFFRG-EGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVD 710
           W+E N KF   +R F +G +G   + A  LCGS +++  GR P  S NFI  + G  L D
Sbjct: 492 WSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLAD 551

Query: 711 LVSFSN-SNLAS----------ELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGV 770
           LV+++N +NLA+            SWNCGEEG  +++ V   R +Q+RNF   L VS GV
Sbjct: 552 LVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGV 611

Query: 771 PVLNMGDECGQSSGGS----VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLF 830
           P++ MGDE G + GG+       N    F WD  K +  +   +F   L  FR     L 
Sbjct: 612 PMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWD-KKEEAHSDFFRFCRILIKFRDECESLG 671

Query: 831 QNRNFLKGENIDWFDNNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFN 851
            N +F   + + W       P W + S +F+A  L  D  + E           I++ FN
Sbjct: 672 LN-DFPTAKRLQWHGLAPEIPNWSETS-RFVAFSL-VDSVKKE-----------IYVAFN 731

BLAST of CSPI07G16810 vs. Swiss-Prot
Match: GLGX_MYCBO (Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) GN=glgX PE=3 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 2.3e-69
Identity = 207/716 (28.91%), Postives = 323/716 (45.11%), Query Frame = 1

Query: 231 GYPSPLGLSISGDGSINFSIFSSSAENLVLCLYNDSTSEK--PLLELDLDPYINRSGNIW 290
           G   PLG +  G G+ NFS+FS  AE + LCL ++   E   PL E+D        G +W
Sbjct: 22  GNAYPLGATYDGAGT-NFSLFSEIAEKVELCLIDEDGVESRIPLDEVD--------GYVW 81

Query: 291 HASFEGASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSI------------- 350
           HA     +    YG++  G          + S++++DPY K                   
Sbjct: 82  HAYLPNITPGQRYGFRVHGPFDPAAGHRCDPSKLLLDPYGKSFHGDFTFGQALYSYDVNA 141

Query: 351 --PKSSGQGLGLPSKFLGQISKVPTFDWDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLP 410
             P S+   +      +  +   P FDW  +  P  P  +  +Y  +V+  T    S +P
Sbjct: 142 VDPDSTPPMVDSLGHTMTSVVINPFFDWAYDRSPRTPYHETVIYEAHVKGMTQTHPS-IP 201

Query: 411 ADIAGTFSGLTKKLL--HFKNLGVNAVLLEPIFQF--DEK--------EGPYFPFHFFSP 470
            ++ GT++GL   ++  H   L V AV L P+ QF  D +           Y  F FF+P
Sbjct: 202 PELRGTYAGLAHPVIIDHLNELNVTAVELMPVHQFLHDSRLLDLGLRNYWGYNTFGFFAP 261

Query: 471 TNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTHTSGNGAL------QGIDDSSY 530
            + Y  +  + SA+   K MV+ LH  G+EVIL+VVY HT+    L      +GID+++Y
Sbjct: 262 HHQYASTRQAGSAVAEFKTMVRSLHEAGIEVILDVVYNHTAEGNHLGPTINFRGIDNTAY 321

Query: 531 YFTNRVANLEEK---------SALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLL 590
           Y   R+ + + +         ++LN  +P   QL++DSLRYWV E HVDGF F  AS L 
Sbjct: 322 Y---RLMDHDLRFYKDFTGTGNSLNARHPHTLQLIMDSLRYWVIEMHVDGFRFDLASTLA 381

Query: 591 RGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFCS 650
           R  H ++     F + +  DP++S+ KL+A+ WD  E   +   FP    W E N K+  
Sbjct: 382 RELH-DVDRLSAFFDLVQQDPVVSQVKLIAEPWDVGEGGYQVGNFP--GLWTEWNGKYRD 441

Query: 651 DIRDFFRGE-GLISSLATRLCGSGDVF-SDGRGPAFSFNFIARNVGLPLVDLVSFSNSNL 710
            +RD++RGE   +   A+RL GS D++ + GR P+ S NF+  + G  L DLVS+++ + 
Sbjct: 442 TVRDYWRGEPATLGEFASRLTGSSDLYEATGRRPSASINFVTAHDGFTLNDLVSYNDKHN 501

Query: 711 AS-----------ELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDEC 770
            +             SWNCG EGPT +  +L  R +Q+RN    L VS G P++  GDE 
Sbjct: 502 EANGENNRDGESYNRSWNCGVEGPTDDPDILALRARQMRNMWATLMVSQGTPMIAHGDEI 561

Query: 771 GQSS-GGSVAFNDKRSFNW-DLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGE-- 830
           G++  G +  +      +W D    D       F    ++ R +   +F+ R F +GE  
Sbjct: 562 GRTQYGNNNVYCQDSELSWMDWSLVDKNADLLAFARKATTLR-KNHKVFRRRRFFEGEPI 621

Query: 831 -------NIDWF---DNNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVF 875
                  +I W        +   W     + +AV L  +               +  + F
Sbjct: 622 RSGDEVRDIAWLTPSGREMTHEDWGRGFDRCVAVFLNGEAITAPDARGERVVDDSFLLCF 681

BLAST of CSPI07G16810 vs. Swiss-Prot
Match: GLGX_MYCTO (Glycogen operon protein GlgX homolog OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) GN=glgX PE=3 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 2.3e-69
Identity = 207/716 (28.91%), Postives = 323/716 (45.11%), Query Frame = 1

Query: 231 GYPSPLGLSISGDGSINFSIFSSSAENLVLCLYNDSTSEK--PLLELDLDPYINRSGNIW 290
           G   PLG +  G G+ NFS+FS  AE + LCL ++   E   PL E+D        G +W
Sbjct: 22  GNAYPLGATYDGAGT-NFSLFSEIAEKVELCLIDEDGVESRIPLDEVD--------GYVW 81

Query: 291 HASFEGASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSI------------- 350
           HA     +    YG++  G          + S++++DPY K                   
Sbjct: 82  HAYLPNITPGQRYGFRVHGPFDPAAGHRCDPSKLLLDPYGKSFHGDFTFGQALYSYDVNA 141

Query: 351 --PKSSGQGLGLPSKFLGQISKVPTFDWDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLP 410
             P S+   +      +  +   P FDW  +  P  P  +  +Y  +V+  T    S +P
Sbjct: 142 VDPDSTPPMVDSLGHTMTSVVINPFFDWAYDRSPRTPYHETVIYEAHVKGMTQTHPS-IP 201

Query: 411 ADIAGTFSGLTKKLL--HFKNLGVNAVLLEPIFQF--DEK--------EGPYFPFHFFSP 470
            ++ GT++GL   ++  H   L V AV L P+ QF  D +           Y  F FF+P
Sbjct: 202 PELRGTYAGLAHPVIIDHLNELNVTAVELMPVHQFLHDSRLLDLGLRNYWGYNTFGFFAP 261

Query: 471 TNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTHTSGNGAL------QGIDDSSY 530
            + Y  +  + SA+   K MV+ LH  G+EVIL+VVY HT+    L      +GID+++Y
Sbjct: 262 HHQYASTRQAGSAVAEFKTMVRSLHEAGIEVILDVVYNHTAEGNHLGPTINFRGIDNTAY 321

Query: 531 YFTNRVANLEEK---------SALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLL 590
           Y   R+ + + +         ++LN  +P   QL++DSLRYWV E HVDGF F  AS L 
Sbjct: 322 Y---RLMDHDLRFYKDFTGTGNSLNARHPHTLQLIMDSLRYWVIEMHVDGFRFDLASTLA 381

Query: 591 RGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFCS 650
           R  H ++     F + +  DP++S+ KL+A+ WD  E   +   FP    W E N K+  
Sbjct: 382 RELH-DVDRLSAFFDLVQQDPVVSQVKLIAEPWDVGEGGYQVGNFP--GLWTEWNGKYRD 441

Query: 651 DIRDFFRGE-GLISSLATRLCGSGDVF-SDGRGPAFSFNFIARNVGLPLVDLVSFSNSNL 710
            +RD++RGE   +   A+RL GS D++ + GR P+ S NF+  + G  L DLVS+++ + 
Sbjct: 442 TVRDYWRGEPATLGEFASRLTGSSDLYEATGRRPSASINFVTAHDGFTLNDLVSYNDKHN 501

Query: 711 AS-----------ELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDEC 770
            +             SWNCG EGPT +  +L  R +Q+RN    L VS G P++  GDE 
Sbjct: 502 EANGENNRDGESYNRSWNCGVEGPTDDPDILALRARQMRNMWATLMVSQGTPMIAHGDEI 561

Query: 771 GQSS-GGSVAFNDKRSFNW-DLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGE-- 830
           G++  G +  +      +W D    D       F    ++ R +   +F+ R F +GE  
Sbjct: 562 GRTQYGNNNVYCQDSELSWMDWSLVDKNADLLAFARKATTLR-KNHKVFRRRRFFEGEPI 621

Query: 831 -------NIDWF---DNNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVF 875
                  +I W        +   W     + +AV L  +               +  + F
Sbjct: 622 RSGDEVRDIAWLTPSGREMTHEDWGRGFDRCVAVFLNGEAITAPDARGERVVDDSFLLCF 681

BLAST of CSPI07G16810 vs. TrEMBL
Match: A0A0A0K551_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G392380 PE=4 SV=1)

HSP 1 Score: 1790.0 bits (4635), Expect = 0.0e+00
Identity = 881/885 (99.55%), Postives = 884/885 (99.89%), Query Frame = 1

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
           MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
           VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFQSSPPDETTGATETPFVKTSEG 180
           TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNF+SSPPDETTGATETPFVKTSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSSAENLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SGDGS+NFSIFSSSAE+LVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
           YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEV PNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480

Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
           NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
           VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
           PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 886
           RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 885

BLAST of CSPI07G16810 vs. TrEMBL
Match: M5VNY8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001199mg PE=4 SV=1)

HSP 1 Score: 1130.2 bits (2922), Expect = 0.0e+00
Identity = 552/891 (61.95%), Postives = 694/891 (77.89%), Query Frame = 1

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
           MAT   S+ IQ  C +NCG+TES KL A++   +  K  + F K+D  R L   E     
Sbjct: 1   MATLPLSIAIQA-CCFNCGITESSKLTATNGFRHRDKAMHGFVKLDAERKLVFRE----V 60

Query: 61  VKSSHRNLS-----KTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFV 120
           V++ + NL      K YAKS +SV    QR   G +++E  +V+TYLFRTE GD+VNVFV
Sbjct: 61  VQNINENLPRDHNLKVYAKSQVSVEPMEQRFSTGTETEEIDKVSTYLFRTEIGDVVNVFV 120

Query: 121 GKKGSTFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTP-NFQSSPPDETTGATETPF 180
            K+ + +TVNIEVPS  L S D  L+L WG+YR+DS+   P +F+SS P++TT   ETP 
Sbjct: 121 RKRNAKYTVNIEVPSSHLSSNDRRLVLRWGMYRADSSCFVPLDFKSSTPNDTTTTLETPL 180

Query: 181 VKTSEGKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPS 240
           ++TS G+F++ELEF+AK  PFY SF+L  P   +    EIRSH+KT+F VPVGFGRGYP 
Sbjct: 181 IQTSSGRFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPG 240

Query: 241 PLGLSISGDGSINFSIFSSSAENLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEG 300
           PLGL+ S DGS+NF+IFS +AE++ LCLY+++T+EKP LELDLDPY+NRSG+IWH SF  
Sbjct: 241 PLGLTFSNDGSMNFAIFSRNAESVALCLYDNTTAEKPALELDLDPYVNRSGDIWHVSFGS 300

Query: 301 ASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQIS 360
           A  FVSYGY+ KG+    N++  +   +++DPYAK++A SIP + G GL    K+LG++ 
Sbjct: 301 AWSFVSYGYKFKGNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGL----KYLGRLC 360

Query: 361 KVPTFDWDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLG 420
           + P FDW G+VRP+L MEKL VYRLNV RFT  KSSQLP +I G+FSGLT+KL HFK+LG
Sbjct: 361 EEPAFDWAGDVRPDLSMEKLVVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDLG 420

Query: 421 VNAVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIL 480
           VNAVLLEPIF FDE++GPYFP HFFSP + +GPS   +SA+NSMKEMV++ HANG+EV+L
Sbjct: 421 VNAVLLEPIFPFDEQKGPYFPHHFFSPMDCFGPSRGPVSAVNSMKEMVRKFHANGIEVLL 480

Query: 481 EVVYTHTSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHV 540
           EVV+THT+   ALQGID SSYY  N V +LE ++ALNCNYPIVQQL+LDSLRYWVTEFHV
Sbjct: 481 EVVFTHTAEGEALQGIDISSYYHVNEVEDLEARNALNCNYPIVQQLVLDSLRYWVTEFHV 540

Query: 541 DGFCFVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHW 600
           DGF F+NAS LLRG +GE LSRPP VEAIAFDPLLSKTK++AD WDP  +  KET FPHW
Sbjct: 541 DGFFFINASSLLRGFNGEYLSRPPLVEAIAFDPLLSKTKIIADCWDPHGMAPKETHFPHW 600

Query: 601 KRWAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLV 660
           +RWAEVN+KFC+D+R+F RGEGL+S LATRLCG+GD+FSDGRGPAF+FNFI+RN GLPLV
Sbjct: 601 RRWAEVNTKFCNDVRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFAFNFISRNSGLPLV 660

Query: 661 DLVSFSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECG 720
           DLVSFS   LASELSWNCGEEGPT+   VLE+RLKQIRNF+F+LFVSLGVPVLNMGDECG
Sbjct: 661 DLVSFSGVELASELSWNCGEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECG 720

Query: 721 QSSGGSVAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWF 780
           QS+GGS A++D+++F+W+ L+T F TQTTQFIAFLSSFR RR DL Q RNFLK ENI W+
Sbjct: 721 QSTGGSPAYSDRKAFDWNALETGFATQTTQFIAFLSSFRKRRSDLLQKRNFLKEENIGWY 780

Query: 781 DNNQSPPQWEDASCKFLAVMLRADKEE-NESITENPKTRSNIFMVFNASDQSESVALPEP 840
           +++Q+PP+WED S KFLA+ L+AD++E N+   E+  +  ++F+ F+A+D SE+V LP P
Sbjct: 781 ESDQTPPRWEDPSRKFLAMRLKADEDEVNQPGDESSHSWGDLFVAFSAADHSETVVLPPP 840

Query: 841 LEGTSWFRVVDTALPFPGFFSSDGELV--PMTGSVTYEIQAHSCALFEAKS 883
           LEG  W R+VDTALPFPGFFS+DGE V   + G   YE+++HSCALFEA+S
Sbjct: 841 LEGMGWRRLVDTALPFPGFFSTDGEPVVEQIVGLFAYEMKSHSCALFEARS 882

BLAST of CSPI07G16810 vs. TrEMBL
Match: A0A067JCG4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21968 PE=4 SV=1)

HSP 1 Score: 1070.5 bits (2767), Expect = 1.2e-309
Identity = 530/886 (59.82%), Postives = 665/886 (75.06%), Query Frame = 1

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
           M T   SL+I+P C YN  V ES KL  + H     +    FG+MD  +    GE     
Sbjct: 1   MTTLPPSLMIRP-CCYNFVVVESSKLIGTAHYISSNRVARGFGRMDVDKRHLAGEIAKNV 60

Query: 61  VKSSHRNL-SKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKG 120
            K+    L S+ +A + + + ++ +      +  E ++V+TYLFR++ G  V VFV KK 
Sbjct: 61  GKTPPWTLHSRAFAAARVPIQQTERMFSTSTEVDEWKKVSTYLFRSQIGGHVKVFVRKKN 120

Query: 121 STFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTP-NFQSSPPDETTGATETPFVKTS 180
               V IEV S+ + + D  L L WG+YRSDSA   P + Q   PD  T   +TPFV+ S
Sbjct: 121 GKHAVYIEVSSLDISTRDYKLTLIWGIYRSDSACFMPLDSQHLDPDART--MDTPFVQNS 180

Query: 181 EGKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGL 240
            G+F++ELEF+AK TP YLSF+LK     DS S EIR+H++T+F VP+GF  GYP+PLGL
Sbjct: 181 FGRFALELEFEAKQTPIYLSFLLKSMFNSDSSSLEIRNHRQTNFCVPIGFSSGYPNPLGL 240

Query: 241 SISGDGSINFSIFSSSAENLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKF 300
           S S DGS+NF+ FS +AE +VLCLY+DST++KP LELDLDPY+NRSG++WHAS E A  F
Sbjct: 241 SFSNDGSMNFAFFSRNAEGVVLCLYDDSTTDKPALELDLDPYVNRSGDVWHASLESAWTF 300

Query: 301 VSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPT 360
            SYGY+CKG+        ++V R+++DPYA+I+   +  ++  G GL +K+LG++ +VP 
Sbjct: 301 TSYGYRCKGTGK------VDVERVLLDPYARII---VNCTADDGSGLSTKYLGRLCEVPA 360

Query: 361 FDWDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAV 420
           F+W  +VRPNL MEKL VYRLNV+ FT  KSS+L +DI GTF+GLT+KL HFKNLGVNAV
Sbjct: 361 FEWGDDVRPNLAMEKLVVYRLNVKHFTESKSSKLYSDIDGTFAGLTEKLNHFKNLGVNAV 420

Query: 421 LLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVY 480
           LLEPIF FDE +GPYFP HFFSP+N YGPSG SISAI SMKEMVKELHANGVEV+LEVV+
Sbjct: 421 LLEPIFPFDEGKGPYFPCHFFSPSNLYGPSGGSISAITSMKEMVKELHANGVEVLLEVVF 480

Query: 481 THTSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFC 540
           THT+  GALQGIDD SYY+ NRV +LE ++ALNCNYPIVQ+++LDSLR+WVTEFH+DGFC
Sbjct: 481 THTAEAGALQGIDDFSYYYANRVVDLESRNALNCNYPIVQRMILDSLRHWVTEFHIDGFC 540

Query: 541 FVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWA 600
           F+NASFLLRG HGE+LSRPP VEAIAFDPLLS TK++AD WDP+++  +E   PHWKRWA
Sbjct: 541 FMNASFLLRGFHGEILSRPPLVEAIAFDPLLSNTKIIADCWDPEDMTPEEICLPHWKRWA 600

Query: 601 EVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVS 660
           E+N+KF SD+R+F RGE L+S LATRLCGSGD+FS GRGPAFSFNFIARN GLPLVDLVS
Sbjct: 601 EINTKFRSDVRNFLRGESLLSDLATRLCGSGDIFSSGRGPAFSFNFIARNSGLPLVDLVS 660

Query: 661 FSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSG 720
           FS+  LASEL WNCGEEGPT+ + VLE+RLKQIRN++F+L+VSLGVPVLNMGDECGQSSG
Sbjct: 661 FSSDELASELCWNCGEEGPTNKIPVLERRLKQIRNYLFILYVSLGVPVLNMGDECGQSSG 720

Query: 721 GSVAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQ 780
           GS+++ D++ F+W+ L T FG Q TQFI+FLSS R+R  D+ Q RNFLK ENIDW+  +Q
Sbjct: 721 GSISYGDRKPFDWNSLSTGFGIQMTQFISFLSSLRTRWSDVLQKRNFLKEENIDWYGTDQ 780

Query: 781 SPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTS 840
           SPP+WED SCKFLA+ LR             K+  N+FM FNA+DQSESV LP+  EG +
Sbjct: 781 SPPRWEDPSCKFLAMTLRT------------KSSGNLFMAFNAADQSESVILPQLPEGMT 840

Query: 841 WFRVVDTALPFPGFFSSDGELV--PMTGSVTYEIQAHSCALFEAKS 883
           W R+VDTALPFPGFFS+DGE V   M   + Y +++HSC LFEA+S
Sbjct: 841 WLRLVDTALPFPGFFSNDGEPVVEQMAELIVYGMKSHSCILFEARS 862

BLAST of CSPI07G16810 vs. TrEMBL
Match: F6HQ64_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0104g00370 PE=4 SV=1)

HSP 1 Score: 1063.9 bits (2750), Expect = 1.0e-307
Identity = 523/886 (59.03%), Postives = 662/886 (74.72%), Query Frame = 1

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
           M T + SLVI+P CV NCG  +S KL A+ H   G K  +   KMD  R    G     A
Sbjct: 1   MTTLIPSLVIRPCCV-NCGARDSSKLVAATHYICGNKVAHGLEKMDLERKEILGVVAQNA 60

Query: 61  VKSSHRNLS-KTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKG 120
            ++  RN++ K  A S I++ ++  R     +S+E      YLF TE G  V V VGKK 
Sbjct: 61  TRNCFRNINWKVSATSHIAIEETENRYSTT-ESEELESRLNYLFLTEIGGQVKVIVGKKN 120

Query: 121 STFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTP-NFQSSPPDETTGATETPFVKTS 180
             + V+IEV S+QL + D  L+LSWGV+RS+S+   P +FQ+  P+  +  TE PF++ S
Sbjct: 121 KKYIVSIEVSSLQLYNSDNKLILSWGVFRSNSSCFMPVDFQNLVPEVGSNTTEIPFMERS 180

Query: 181 EGKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGL 240
            G F+++L+F+A H PFYLSF+LK  +  D  SS+IRSH+KT+F +PVGFGRGYP+PLGL
Sbjct: 181 SGSFALKLDFEANHAPFYLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGFGRGYPAPLGL 240

Query: 241 SISGDGSINFSIFSSSAENLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKF 300
           S S DGS NF+ FS +A  +VLCLY D TS+KP LE+DLDPY+NR+G+IWHAS E    F
Sbjct: 241 SFSSDGSPNFAFFSRNAGGVVLCLY-DGTSDKPALEIDLDPYVNRTGDIWHASMESVGSF 300

Query: 301 VSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPT 360
           VSYGY+CK +   ++ + L    + +DPYAK++  S   S   GL  P   LG++ K P 
Sbjct: 301 VSYGYRCKEANLQDSGETLHTEHVHLDPYAKLIRNSF--SDDHGLK-PQPRLGELQKEPA 360

Query: 361 FDWDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAV 420
           F+W+ +V P +PMEKL VYRLNV  FT D+SSQ+ +D+AGTFSGL +KL HFK+LGVNAV
Sbjct: 361 FNWNDDVHPYIPMEKLVVYRLNVMHFTKDESSQVASDLAGTFSGLMEKLHHFKDLGVNAV 420

Query: 421 LLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVY 480
           LLEPIF FDE++GPYFPFHFFSP N YGPS   +S INS+KEMVK LHANG+EV+LEVV+
Sbjct: 421 LLEPIFSFDEQKGPYFPFHFFSPMNVYGPSSGPVSTINSVKEMVKRLHANGIEVLLEVVF 480

Query: 481 THTSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFC 540
           THT+ +GALQGIDDS YY+ N  A+L  ++ALNCNY IVQQ+++DSLRYWVTEFHVDGFC
Sbjct: 481 THTAESGALQGIDDSCYYYVNGDADLGIRNALNCNYSIVQQMIVDSLRYWVTEFHVDGFC 540

Query: 541 FVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWA 600
           F+NAS LLRG HGE LSRPP VE IAFDPLLSKTK++AD WDP+ +  KE RFPHWKRWA
Sbjct: 541 FINASSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADCWDPRNMLPKEIRFPHWKRWA 600

Query: 601 EVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVS 660
           EVN++FC+D+R+F RGEGL S  ATRLCGSGD+F DGRGPAFSFNF  +N GLPLVDLVS
Sbjct: 601 EVNTRFCNDVRNFLRGEGL-SDFATRLCGSGDIFMDGRGPAFSFNFTTKNFGLPLVDLVS 660

Query: 661 FSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSG 720
           FS+S LASELSWNCG+EGPT+   VLE+RLKQIRNF+F+L+VSLGVP+LNMGDECGQSSG
Sbjct: 661 FSSSELASELSWNCGDEGPTNKTTVLERRLKQIRNFLFILYVSLGVPILNMGDECGQSSG 720

Query: 721 GSVAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQ 780
           GS A+ D++ FNW+ +KT FG QT QFI+FLSS RSRR DL Q R+FLK E+IDW  ++Q
Sbjct: 721 GSPAYGDRKPFNWNSVKTGFGIQTIQFISFLSSLRSRRSDLLQRRSFLKEESIDWHGSDQ 780

Query: 781 SPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTS 840
           SPP+W+D S KFLA+ L+A+  E +  +E+   + ++F+ FN +D+S  V LP P  G  
Sbjct: 781 SPPRWDDPSSKFLAMTLKAENMEGQLPSESSSIKGDLFIAFNTADRSVKVILPPPPTGMV 840

Query: 841 WFRVVDTALPFPGFFSSDGE--LVPMTGSVTYEIQAHSCALFEAKS 883
           W R+VDTALPFPGFF++DGE  L   +G VTY++++HSCALFEA +
Sbjct: 841 WHRLVDTALPFPGFFTADGEAILKKKSGLVTYKMESHSCALFEANT 879

BLAST of CSPI07G16810 vs. TrEMBL
Match: W9QT37_9ROSA (Isoamylase 2 OS=Morus notabilis GN=L484_025032 PE=4 SV=1)

HSP 1 Score: 1056.6 bits (2731), Expect = 1.7e-305
Identity = 525/828 (63.41%), Postives = 649/828 (78.38%), Query Frame = 1

Query: 59  GAVKSSHRNLS-KTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGK 118
           G  +   RNLS +  A S +S+    +RL    K +E  +V+TYLFRTE G LVNVFV K
Sbjct: 10  GNFEKMPRNLSVEVNATSRVSIEPVQKRLSRNTKVEEAEKVSTYLFRTEVGGLVNVFVRK 69

Query: 119 KGSTFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPN-FQSSPPDETTGATETPFVK 178
           K    TV +EV S++L   D  L LSWG+YRSDS+   P+ F++S P       ETPF K
Sbjct: 70  KKVNCTVYVEVSSLELPRSDGTLGLSWGIYRSDSSSFLPSHFETSTP------VETPFTK 129

Query: 179 TSEGKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPL 238
            S G+++VE EF+AK  P YLSF+LK P   DS   +IRSH+KT+F VP+GF  G+P+PL
Sbjct: 130 NSFGRYTVEFEFEAKEIPCYLSFLLKSPRDNDSSGLDIRSHRKTNFCVPLGFSSGHPTPL 189

Query: 239 GLSISGDGSINFSIFSSSAENLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGAS 298
           GLS S DGS+NFSIFS SAE++VLCLY+D+T++ P LELDLDPY+NR+G+IWHASFE +S
Sbjct: 190 GLSFSRDGSLNFSIFSRSAESVVLCLYDDTTADNPALELDLDPYVNRTGDIWHASFESSS 249

Query: 299 KFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKV 358
            FVSYGY+ KGS+    +D  E +RIV+DPYAKI+  S   SS  G+G   K+LG+ISK 
Sbjct: 250 TFVSYGYRLKGSRLKGKKD--EDARIVLDPYAKIIGKST--SSDHGIG--PKYLGRISKE 309

Query: 359 PTFDWDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVN 418
           P FDW G++RPNLP+EKL VYRLNV  FT  KSSQL  DIAGTFSGL +KL HF +LGVN
Sbjct: 310 PGFDWSGDIRPNLPIEKLTVYRLNVMHFTEHKSSQLSPDIAGTFSGLIEKLEHFTDLGVN 369

Query: 419 AVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEV 478
           AVLLEPIF FDE++GP FP HFFSPT+ YGPSG ++SAIN+MKEMVK+LHA+G+EV+LEV
Sbjct: 370 AVLLEPIFPFDEQKGPCFPCHFFSPTSLYGPSGGAVSAINTMKEMVKKLHASGIEVLLEV 429

Query: 479 VYTHTSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDG 538
           V+TH++ +GALQGIDDSSYY    VA+LE ++ LNCNYPIVQQ +LDSLRYWVTEFHVDG
Sbjct: 430 VFTHSAESGALQGIDDSSYYLKG-VADLEARNVLNCNYPIVQQFILDSLRYWVTEFHVDG 489

Query: 539 FCFVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKR 598
           FCF+NAS LL+G  GE LS PP VEAIAFDPLLSKTK++AD WDP     +ETRFPHWK 
Sbjct: 490 FCFINASLLLQGSRGEYLSHPPLVEAIAFDPLLSKTKIIADCWDPHNTVLEETRFPHWKT 549

Query: 599 WAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDL 658
           WAE+N KFC+D+R+F RGEGL+SSLATRLCGSGD+FS GRGPAFSFNFI+RN GLPLVDL
Sbjct: 550 WAEMNMKFCNDVRNFLRGEGLLSSLATRLCGSGDIFSKGRGPAFSFNFISRNSGLPLVDL 609

Query: 659 VSFSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQS 718
           VSFS+  LASELSWNCGEEGPT+   VLE+RLKQ RNF+FVL+VSLGVPVLNMGDECGQS
Sbjct: 610 VSFSSDKLASELSWNCGEEGPTNKTLVLERRLKQTRNFLFVLYVSLGVPVLNMGDECGQS 669

Query: 719 SGGSVAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDN 778
           +GGS A++D++SF+W+ LK+ FG QTT+FI+FLSS R RR DL QN N+LK ENIDW  +
Sbjct: 670 TGGSPAYSDRKSFDWNALKSSFGIQTTEFISFLSSLRRRRSDLLQNMNYLKEENIDWHGS 729

Query: 779 NQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEG 838
           +QSPP+WED +CKFLA+ LR D++++++  +    + ++F+ FNA+D SESV LP   EG
Sbjct: 730 DQSPPRWEDPTCKFLAMRLRVDEDKDKAENQTTSGKGDLFIAFNAADLSESVILPPIAEG 789

Query: 839 TSWFRVVDTALPFPGFFSSDGELVP--MTGSVTYEIQAHSCALFEAKS 883
            +W R+VDTALPFPGFF +DGE VP  + G + YE+++ S  LFEA+S
Sbjct: 790 MAWHRLVDTALPFPGFFLTDGEPVPENVDGLLAYEMKSLSSTLFEARS 824

BLAST of CSPI07G16810 vs. TAIR10
Match: AT1G03310.2 (AT1G03310.2 debranching enzyme 1)

HSP 1 Score: 943.7 bits (2438), Expect = 8.0e-275
Identity = 476/881 (54.03%), Postives = 629/881 (71.40%), Query Frame = 1

Query: 14  CVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGAVKSSHRNLSKTYA 73
           C  N G+T + K  ++   T G+K K + G   E  M          V S+     ++Y 
Sbjct: 13  CCLNNGITRTWKFPSARLFT-GRKNKIKLGS--ETLMFTRKRFMGDLVTSA----LQSYQ 72

Query: 74  KSGISVGKSGQRLGIGGKSK-----EQRRVATYLFRTEFGDLVNVFVGKKGSTFTVNIEV 133
            S I   K+   L     S+     + ++V +Y FRT+ G LV V V KK   +++ + V
Sbjct: 73  FSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYV 132

Query: 134 PSMQLVSIDEA-LLLSWGVYRSDSALVTP-NFQSSPPDETTGATETPFVKTSEGKFSVEL 193
            S++L   D++ L++ WGVYRSDS+   P +F++S  D  T  TET FVK+S  +  + L
Sbjct: 133 SSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGL 192

Query: 194 EFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSIS-GDGS 253
           EFD K +PFYLSF LK   G D    E+ +H+ T F +PVGF  G+P PLGLS    D S
Sbjct: 193 EFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDS 252

Query: 254 INFSIFSSSAENLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVSYGYQC 313
            NFS FS S+ N+VLCLY+DST++KP LELDLDPY+NR+G++WHAS +    FV YGY+C
Sbjct: 253 WNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRC 312

Query: 314 KGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFDWDGEV 373
           K +   +    +E   IV+DPYA ++  S+ +          K+LG +SK P+FDW  +V
Sbjct: 313 KETAHSKEDVDVEGEPIVLDPYATVVGKSVSQ----------KYLGSLSKSPSFDWGEDV 372

Query: 374 RPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQ 433
            PN+P+EKL VYRLNV+ FT  +SS+LP+++AGTFSG+ +K+ H K LG NAVLLEPIF 
Sbjct: 373 SPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFS 432

Query: 434 FDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTHTSGNG 493
           F E++GPYFPFHFFSP + YGPS +  SA+NSMK MVK+LH+ G+EV+LEVV+THT+ +G
Sbjct: 433 FSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG 492

Query: 494 ALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFL 553
           AL+GIDDSSYY+  R  +L+ KS LNCNYP+VQQL+L+SLRYWVTEFHVDGFCF+NAS L
Sbjct: 493 ALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSL 552

Query: 554 LRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFC 613
           LRG HGE LSRPP VEAIAFDPLL++TKL+AD WDP E+  KE RFPHWKRWAE+N+++C
Sbjct: 553 LRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYC 612

Query: 614 SDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSNSNLA 673
            ++R+F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I+RN GL LVD+VSFS   LA
Sbjct: 613 RNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELA 672

Query: 674 SELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFND 733
           SELSWNCGEEG T+   VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS     
Sbjct: 673 SELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLES 732

Query: 734 KRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSPPQWED 793
           ++ F+W+LL + FGTQ TQFI+F++S R+RR D+FQ R+FLK ENI W+ N+Q+ P+WED
Sbjct: 733 RKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWED 792

Query: 794 ASCKFLAVMLRADKEENE--SITENPKTRSN-IFMVFNASDQSESVALPEPLEGTSWFRV 853
            + KFLA+ ++++ EE E  S+ E  + +SN +F+ FNASD  ESV LP   +G+ W R+
Sbjct: 793 PASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRL 852

Query: 854 VDTALPFPGFFSSDGELV----PMTGSVTYEIQAHSCALFE 880
           VDTALPFPGFFS +GE V    P+   V YE++ +SC LFE
Sbjct: 853 VDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of CSPI07G16810 vs. TAIR10
Match: AT4G09020.1 (AT4G09020.1 isoamylase 3)

HSP 1 Score: 373.6 bits (958), Expect = 3.3e-103
Identity = 246/701 (35.09%), Postives = 362/701 (51.64%), Query Frame = 1

Query: 231 GYPSPLGLSISGDGSINFSIFSSSAENLVLCLY-----NDSTSEKPLLELDLDPYINRSG 290
           G  SPLG+S   D  INF++FS +A ++ LCL       D T +  ++EL LDP +N++G
Sbjct: 88  GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 291 NIWHASFEGAS-KFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLG 350
           + WH   E      V YGY+  G    +     + S +++DPYAK++      S G    
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGH--SSFGDSSQ 207

Query: 351 LPSKFLGQIS-KVPTFDWDGEVR-PNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSG 410
             ++F G    +   FDW  + + PN+P + L +Y +NV  FT D+SS +   I G++ G
Sbjct: 208 KFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267

Query: 411 LTKKLLHFKNLGVNAVLLEPIFQFDEKE-----GP---------YFPFHFFSPTNNYGPS 470
             +K+ H ++LG+NAV L P+F+FDE E      P         Y   +FF+P + Y   
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327

Query: 471 -GASISAINSMKEMVKELHANGVEVILEVVYTHTSG-------NGALQGIDDSSYYF--- 530
            G  I A    KEMVK LH+ G+EVIL+VVY HT+          + +GID+  YY    
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387

Query: 531 TNRVANLEE-KSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLRGHHGELLSR 590
            N++ N     + LNCN+P+V +L+LDSLR+WVTE+HVDGF F  AS L R   G  LS 
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447

Query: 591 PPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFCSDIRDFFRGE- 650
           PP + AIA D +LS+ K++A+ WD   L     +FP+W RWAE N  +  D+R F +G+ 
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWDCGGL-YLVGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507

Query: 651 GLISSLATRLCGSGDVFS-DGRGPAFSFNFIARNVGLPLVDLVSFS-----------NSN 710
           G+  S ATR+ GS D++  + R P    NF+  + G  L DLVS++           N  
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567

Query: 711 LASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSS-GGSVA 770
                SWNCG EG T +  +   R +Q++NF   L +S G P++ MGDE G +  G + +
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNS 627

Query: 771 FNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRR-FDLFQNRNFLKGENIDWFDNNQSPP 830
           +    S N    K     +   F  F    + R    + ++ NFL    I W ++N    
Sbjct: 628 YGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWHEDN---- 687

Query: 831 QWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWFR 882
            W+++  KFLA  L      ++ I        +I++ FNA D      +P+P  G  WFR
Sbjct: 688 -WDNSESKFLAFTL------HDGIGGR-----DIYVAFNAHDYFVKALIPQPPPGKQWFR 747

BLAST of CSPI07G16810 vs. TAIR10
Match: AT2G39930.1 (AT2G39930.1 isoamylase 1)

HSP 1 Score: 347.8 bits (891), Expect = 1.9e-95
Identity = 239/690 (34.64%), Postives = 344/690 (49.86%), Query Frame = 1

Query: 231 GYPSPLGLSISGDGSINFSIFSSSAENLVLCLYN--DSTSEKPLLELDLDPYINRSGNIW 290
           G PSP G ++  DG +NFS++S+++ +  +CL +  D    K   E+ LDP  NR+G++W
Sbjct: 72  GLPSPFGPTVRDDG-VNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVW 131

Query: 291 HASFEGASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSK 350
           H    G  K + YGY+  G  S E     + S I++DPYAK +   I +     LG    
Sbjct: 132 HVFLRGDFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAI---ISRDEFGVLGPDDN 191

Query: 351 FLGQIS-KVPT----FDWDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGL 410
              Q++  VPT    FDW+G++   LP + L +Y ++V  FT  +SS++  +  GT+ G+
Sbjct: 192 CWPQMACMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQGV 251

Query: 411 TKKLLHFKNLGVNAVLLEPIFQFDEKE---------------GPYFPFHFFSPTNNYGPS 470
            +KL H K LG+N + L P  +F+E E                 Y    FFSP   Y  +
Sbjct: 252 AEKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASA 311

Query: 471 GASI---SAINSMKEMVKELHANGVEVILEVVYTHTS-GNG-----ALQGIDDSSYYFTN 530
            ++     AIN  K +VKE H  G+EVI++VV  HT+ GN      + +G+D+S YY   
Sbjct: 312 SSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLA 371

Query: 531 RVANLEEKSA----LNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLRGH------ 590
                   S      NCN+P+V+Q +LD LRYWVTE HVDGF F   S + R        
Sbjct: 372 PKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAA 431

Query: 591 -------HGELL------SRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKR 650
                   G+LL      S PP ++ I+ DP+L   KL+A+ WD   L  +   FPHW  
Sbjct: 432 NVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLY-QVGMFPHWGI 491

Query: 651 WAEVNSKFCSDIRDFFRG-EGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVD 710
           W+E N KF   +R F +G +G   + A  LCGS +++  GR P  S NFI  + G  L D
Sbjct: 492 WSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLAD 551

Query: 711 LVSFSN-SNLAS----------ELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGV 770
           LV+++N +NLA+            SWNCGEEG  +++ V   R +Q+RNF   L VS GV
Sbjct: 552 LVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGV 611

Query: 771 PVLNMGDECGQSSGGS----VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLF 830
           P++ MGDE G + GG+       N    F WD  K +  +   +F   L  FR     L 
Sbjct: 612 PMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWD-KKEEAHSDFFRFCRILIKFRDECESLG 671

Query: 831 QNRNFLKGENIDWFDNNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFN 851
            N +F   + + W       P W + S +F+A  L  D  + E           I++ FN
Sbjct: 672 LN-DFPTAKRLQWHGLAPEIPNWSETS-RFVAFSL-VDSVKKE-----------IYVAFN 731

BLAST of CSPI07G16810 vs. TAIR10
Match: AT5G04360.1 (AT5G04360.1 limit dextrinase)

HSP 1 Score: 63.2 bits (152), Expect = 9.6e-10
Identity = 83/366 (22.68%), Postives = 142/366 (38.80%), Query Frame = 1

Query: 232 YPSPLGLSISGDGSINFSIFSSSAENLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHAS 291
           Y  PLG   + +G ++  +++ +A+ + +C+Y +   + P+   ++ P +  +  +W  S
Sbjct: 204 YDGPLGAHFTPEG-VSLHLWAPTAQAVSVCIYKNPLDKSPM---EICP-LKEANGVW--S 263

Query: 292 FEGASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLG 351
            EGA  +    Y  K S  H +   LE      DPYA+ L+    K+          FL 
Sbjct: 264 TEGACSWGGCYYVYKVSVYHPSTMKLETC-YANDPYARGLSADGRKT----------FLV 323

Query: 352 QISK--VPTFDWDG--EVRPNL-PMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKK 411
            +    +    WD   + +P L     + +Y L+V  F+ +  +  P +  G  +  +K 
Sbjct: 324 NLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPENRGGYLAFTSKD 383

Query: 412 LLHFKNL------GVNAVLLEPIF----QFDEKEG------------------------- 471
               K+L      G+  + L P F      DEKE                          
Sbjct: 384 SAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQARITE 443

Query: 472 ---------PYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTHTS 531
                     Y P  +  P  +Y         I   ++MV+ L+  G+ V+L+VVY H  
Sbjct: 444 IQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRIIEFRKMVQALNCTGLNVVLDVVYNHLH 503

Query: 532 GNG------ALQGIDDSSYYFTNRVANLEEKSALN---CNYPIVQQLLLDSLRYWVTEFH 540
            +G       L  I    Y   N    +E  + +N     + +V +L+ D L  WV  + 
Sbjct: 504 ASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIRDDLLNWVVNYK 551

BLAST of CSPI07G16810 vs. NCBI nr
Match: gi|449436816|ref|XP_004136188.1| (PREDICTED: isoamylase 2, chloroplastic [Cucumis sativus])

HSP 1 Score: 1790.0 bits (4635), Expect = 0.0e+00
Identity = 881/885 (99.55%), Postives = 884/885 (99.89%), Query Frame = 1

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
           MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
           VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFQSSPPDETTGATETPFVKTSEG 180
           TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNF+SSPPDETTGATETPFVKTSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSSAENLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SGDGS+NFSIFSSSAE+LVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
           YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEV PNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480

Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
           NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
           VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
           PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 886
           RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 885

BLAST of CSPI07G16810 vs. NCBI nr
Match: gi|659101035|ref|XP_008451396.1| (PREDICTED: isoamylase 2, chloroplastic [Cucumis melo])

HSP 1 Score: 1690.2 bits (4376), Expect = 0.0e+00
Identity = 832/884 (94.12%), Postives = 853/884 (96.49%), Query Frame = 1

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
           MATFLSSLVIQPFCV+NCGVTES KLA SDHLTYGQKTKYQ GKMDEARMLAHGE+KVGA
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
           VKSSHRNLSK Y  SGI VGKSGQRLGIGGKSKEQR+VATYLFRTEFGDLVNVFVGK GS
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFQSSPPDETTGATETPFVKTSEG 180
            FTVNIE+ SMQLVS DE LLLSW VYRSDSA VT NFQSSPPD TTGATETPFVKTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
           KFSVELEFDAKHTPFYLSFVLK+PMGVDSGSSEIRSH+KTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSSAENLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
           SGDGSINFSIFSS+AE++VLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGAS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
           YGYQCKG KSHENQDGLEVSRIVVDPYAKILAPSIPKS  QGLG P KFLGQIS VP FD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
           WDGEVRPNLPMEKLFVYRLNV+RFT+DKSSQLPADIAGTFSGLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
           EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVI+EVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
           TS +GALQGIDDSSYYF NRVANLEE+SALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
           NASFLL+GHHGELLSRPPFVEAIAFDP+LSKTKLVADFWDPQEL SKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
           NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
           NSNLASELSWNCGEEGPT+ LKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
           VAFND+RSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQ+RNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
           PQWEDASCKFLAVMLRADKEENESITENPKTRS+IFM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 885
           RVVDTALPFPGFFSSDGELVPMTGSVTYE+QAHSCALFEAKSAN
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

BLAST of CSPI07G16810 vs. NCBI nr
Match: gi|595800946|ref|XP_007201750.1| (hypothetical protein PRUPE_ppa001199mg [Prunus persica])

HSP 1 Score: 1130.2 bits (2922), Expect = 0.0e+00
Identity = 552/891 (61.95%), Postives = 694/891 (77.89%), Query Frame = 1

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
           MAT   S+ IQ  C +NCG+TES KL A++   +  K  + F K+D  R L   E     
Sbjct: 1   MATLPLSIAIQA-CCFNCGITESSKLTATNGFRHRDKAMHGFVKLDAERKLVFRE----V 60

Query: 61  VKSSHRNLS-----KTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFV 120
           V++ + NL      K YAKS +SV    QR   G +++E  +V+TYLFRTE GD+VNVFV
Sbjct: 61  VQNINENLPRDHNLKVYAKSQVSVEPMEQRFSTGTETEEIDKVSTYLFRTEIGDVVNVFV 120

Query: 121 GKKGSTFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTP-NFQSSPPDETTGATETPF 180
            K+ + +TVNIEVPS  L S D  L+L WG+YR+DS+   P +F+SS P++TT   ETP 
Sbjct: 121 RKRNAKYTVNIEVPSSHLSSNDRRLVLRWGMYRADSSCFVPLDFKSSTPNDTTTTLETPL 180

Query: 181 VKTSEGKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPS 240
           ++TS G+F++ELEF+AK  PFY SF+L  P   +    EIRSH+KT+F VPVGFGRGYP 
Sbjct: 181 IQTSSGRFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPG 240

Query: 241 PLGLSISGDGSINFSIFSSSAENLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEG 300
           PLGL+ S DGS+NF+IFS +AE++ LCLY+++T+EKP LELDLDPY+NRSG+IWH SF  
Sbjct: 241 PLGLTFSNDGSMNFAIFSRNAESVALCLYDNTTAEKPALELDLDPYVNRSGDIWHVSFGS 300

Query: 301 ASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQIS 360
           A  FVSYGY+ KG+    N++  +   +++DPYAK++A SIP + G GL    K+LG++ 
Sbjct: 301 AWSFVSYGYKFKGNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGL----KYLGRLC 360

Query: 361 KVPTFDWDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLG 420
           + P FDW G+VRP+L MEKL VYRLNV RFT  KSSQLP +I G+FSGLT+KL HFK+LG
Sbjct: 361 EEPAFDWAGDVRPDLSMEKLVVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDLG 420

Query: 421 VNAVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIL 480
           VNAVLLEPIF FDE++GPYFP HFFSP + +GPS   +SA+NSMKEMV++ HANG+EV+L
Sbjct: 421 VNAVLLEPIFPFDEQKGPYFPHHFFSPMDCFGPSRGPVSAVNSMKEMVRKFHANGIEVLL 480

Query: 481 EVVYTHTSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHV 540
           EVV+THT+   ALQGID SSYY  N V +LE ++ALNCNYPIVQQL+LDSLRYWVTEFHV
Sbjct: 481 EVVFTHTAEGEALQGIDISSYYHVNEVEDLEARNALNCNYPIVQQLVLDSLRYWVTEFHV 540

Query: 541 DGFCFVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHW 600
           DGF F+NAS LLRG +GE LSRPP VEAIAFDPLLSKTK++AD WDP  +  KET FPHW
Sbjct: 541 DGFFFINASSLLRGFNGEYLSRPPLVEAIAFDPLLSKTKIIADCWDPHGMAPKETHFPHW 600

Query: 601 KRWAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLV 660
           +RWAEVN+KFC+D+R+F RGEGL+S LATRLCG+GD+FSDGRGPAF+FNFI+RN GLPLV
Sbjct: 601 RRWAEVNTKFCNDVRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFAFNFISRNSGLPLV 660

Query: 661 DLVSFSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECG 720
           DLVSFS   LASELSWNCGEEGPT+   VLE+RLKQIRNF+F+LFVSLGVPVLNMGDECG
Sbjct: 661 DLVSFSGVELASELSWNCGEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECG 720

Query: 721 QSSGGSVAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWF 780
           QS+GGS A++D+++F+W+ L+T F TQTTQFIAFLSSFR RR DL Q RNFLK ENI W+
Sbjct: 721 QSTGGSPAYSDRKAFDWNALETGFATQTTQFIAFLSSFRKRRSDLLQKRNFLKEENIGWY 780

Query: 781 DNNQSPPQWEDASCKFLAVMLRADKEE-NESITENPKTRSNIFMVFNASDQSESVALPEP 840
           +++Q+PP+WED S KFLA+ L+AD++E N+   E+  +  ++F+ F+A+D SE+V LP P
Sbjct: 781 ESDQTPPRWEDPSRKFLAMRLKADEDEVNQPGDESSHSWGDLFVAFSAADHSETVVLPPP 840

Query: 841 LEGTSWFRVVDTALPFPGFFSSDGELV--PMTGSVTYEIQAHSCALFEAKS 883
           LEG  W R+VDTALPFPGFFS+DGE V   + G   YE+++HSCALFEA+S
Sbjct: 841 LEGMGWRRLVDTALPFPGFFSTDGEPVVEQIVGLFAYEMKSHSCALFEARS 882

BLAST of CSPI07G16810 vs. NCBI nr
Match: gi|645258481|ref|XP_008234909.1| (PREDICTED: isoamylase 2, chloroplastic [Prunus mume])

HSP 1 Score: 1126.7 bits (2913), Expect = 0.0e+00
Identity = 551/891 (61.84%), Postives = 693/891 (77.78%), Query Frame = 1

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
           MAT   S+ IQ  C+ NCG+TES KL A++   +  K  + F K+D  R L   E     
Sbjct: 1   MATLPLSIAIQACCL-NCGITESSKLTATNGFRHRNKAMHGFVKLDVERKLVFRE----V 60

Query: 61  VKSSHRNLS-----KTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFV 120
           V++ + NL      K YAKS +SV    QR   G +++E  +V+TYLFRTE GD+VNVFV
Sbjct: 61  VQNFNENLPRDHNLKVYAKSQVSVEPMEQRFSTGTETEEIDKVSTYLFRTEIGDVVNVFV 120

Query: 121 GKKGSTFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTP-NFQSSPPDETTGATETPF 180
            K+ + + VNIEVPS+ L S D  L+L WG+YR+DS+   P +F+SS P++TT   ETPF
Sbjct: 121 RKRNAKYIVNIEVPSLYLSSSDRRLVLHWGMYRADSSCFMPLDFKSSTPNDTTTTLETPF 180

Query: 181 VKTSEGKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPS 240
           ++TS G+F++ELEF+AK  PFY SF+L  P   +    EIRSH+KT+F VPVGFGRGYP 
Sbjct: 181 IQTSSGRFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPG 240

Query: 241 PLGLSISGDGSINFSIFSSSAENLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEG 300
           PLGL+ S DGS+NF+IFS +AE++VLCLY+++T+EKP LELDLDPY+NRSG+IWH SF  
Sbjct: 241 PLGLTFSNDGSMNFAIFSRNAESVVLCLYDNTTAEKPALELDLDPYVNRSGDIWHVSFGS 300

Query: 301 ASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQIS 360
           A  FVSYGY+ K +    N++  +   +++DPYAK++A SIP + G GL    K+LG++ 
Sbjct: 301 AWTFVSYGYKFKRNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGL----KYLGRLC 360

Query: 361 KVPTFDWDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLG 420
           + P FDW G+VRP+L MEKL VYRLNV RFT  KSSQLP +I G+FSGLT+KL HFK+LG
Sbjct: 361 EEPAFDWAGDVRPDLSMEKLVVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDLG 420

Query: 421 VNAVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIL 480
           VNAVLLEPIF FDE++GPYFP HFFSP    GPS   +SA+NSMKEMV++ HANG+EV+L
Sbjct: 421 VNAVLLEPIFPFDEQKGPYFPRHFFSPMECIGPSRGPVSAVNSMKEMVRKFHANGIEVLL 480

Query: 481 EVVYTHTSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHV 540
           EVV+THT+   ALQGID SSYY  N V +LE ++ALNCNYPIVQQL+LDSLRYWVTEFH+
Sbjct: 481 EVVFTHTAEGEALQGIDISSYYHVNEVEDLEARNALNCNYPIVQQLVLDSLRYWVTEFHI 540

Query: 541 DGFCFVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHW 600
           DGFCF+NAS LLRG +GE LSRPP VEAIAFDPLLSKTK++AD WDP  +  KETRFPHW
Sbjct: 541 DGFCFINASSLLRGFNGEYLSRPPLVEAIAFDPLLSKTKIIADCWDPHGMAPKETRFPHW 600

Query: 601 KRWAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLV 660
           KRWAEVN+KFC+D+R++ RG+GL+S LATRLCG+GD+FSDGRGPAF+FNFI+RN GLPLV
Sbjct: 601 KRWAEVNTKFCNDVRNYLRGKGLLSDLATRLCGNGDIFSDGRGPAFAFNFISRNSGLPLV 660

Query: 661 DLVSFSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECG 720
           DLVSFS   LASELSWNCGEEGPT+   VLE+RLKQIRNF+F+LFVSLGVPVLN GDECG
Sbjct: 661 DLVSFSGVELASELSWNCGEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPVLNTGDECG 720

Query: 721 QSSGGSVAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWF 780
           QS+GGS A++D+++F+W+ L+T F TQTTQFIAFLSSFR RR DL Q RNFLK ENIDW+
Sbjct: 721 QSTGGSPAYSDRKAFDWNALETGFATQTTQFIAFLSSFRKRRSDLLQKRNFLKEENIDWY 780

Query: 781 DNNQSPPQWEDASCKFLAVMLRADKEE-NESITENPKTRSNIFMVFNASDQSESVALPEP 840
           +++Q+PP+WED S KFLA+ L+AD++E N+   E+  +  ++F  F+A+D SE++ LP P
Sbjct: 781 ESDQTPPRWEDPSRKFLAMRLKADEDEVNQPGDESSHSWGDLFAAFSAADHSETLVLPPP 840

Query: 841 LEGTSWFRVVDTALPFPGFFSSDGELV--PMTGSVTYEIQAHSCALFEAKS 883
            EG  W R+VDTALPFPGFFS+DGE V   M G   YE+++HSCALFEA+S
Sbjct: 841 REGMGWRRLVDTALPFPGFFSTDGEPVVRQMVGLFAYEMKSHSCALFEARS 882

BLAST of CSPI07G16810 vs. NCBI nr
Match: gi|694441961|ref|XP_009347699.1| (PREDICTED: isoamylase 2, chloroplastic-like [Pyrus x bretschneideri])

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 549/887 (61.89%), Postives = 674/887 (75.99%), Query Frame = 1

Query: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGEN-KVG 60
           MAT   S+ IQ  C  NCG TE PKL A D   +     + + K+   R L   E  ++ 
Sbjct: 1   MATLPLSIAIQASCCLNCGTTELPKLTAMDRYRHRNNGVHGYVKLGTERNLVLREVVQIF 60

Query: 61  AVKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKG 120
             +       K YA S +SV    QR+  G +++E  +V+TYLFRT+ GDLVNVFV K+ 
Sbjct: 61  KERPLRDREWKVYATSRVSVEPMEQRVSTGTETEEPSKVSTYLFRTDTGDLVNVFVRKRN 120

Query: 121 STFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTP-NFQSSPPDETTGATETPFVKTS 180
           + +TVNIEV  +     D  L+LSWG+YR+DS+   P NF+SS P + T A ETPF  TS
Sbjct: 121 AKYTVNIEVSLLHPSRSDRRLVLSWGMYRADSSSFVPSNFRSSTPADRTTALETPFTGTS 180

Query: 181 EGKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGL 240
            GKF++ELEF+AK  PFYLSFVLK P   D    EIRSH+KT+F  PVGFGRG P+PLGL
Sbjct: 181 SGKFTLELEFEAKQIPFYLSFVLKSPADADLSDLEIRSHRKTNFCFPVGFGRGCPAPLGL 240

Query: 241 SISGDGSINFSIFSSSAENLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKF 300
           S S DGS+NF+IFS +AE++VLCLY+++T+EKP LELDLDPY+NRSG+IWHASFE A  F
Sbjct: 241 SFSNDGSMNFAIFSRNAESVVLCLYDETTAEKPALELDLDPYVNRSGDIWHASFEKAWDF 300

Query: 301 VSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPT 360
           VSYGY+             +   +++DPYAKI+A S+P  +G      SK+LG++ + P 
Sbjct: 301 VSYGYR------------FDEGNVLLDPYAKIIAKSVPHGTG------SKYLGRLREEPA 360

Query: 361 FDWDGEVRPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAV 420
           FDW G+V PNL +EKL VYRLNV+RFT  KSS+LP +IAGTFSGLT+KL HFK LGVNAV
Sbjct: 361 FDWAGDVLPNLTLEKLVVYRLNVKRFTEHKSSKLPTNIAGTFSGLTQKLEHFKALGVNAV 420

Query: 421 LLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVY 480
           LLEPIF FDE +GPYFP HFFSP N +GPSG  +SA+NSMKE VK+ HA+GVEV+LEVV+
Sbjct: 421 LLEPIFPFDEHKGPYFPVHFFSPMNCFGPSGP-VSAVNSMKETVKKFHADGVEVLLEVVF 480

Query: 481 THTSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFC 540
           THT+   A+QGID SSYY  N V +LE ++ALNCNYP+VQQ++LDSLRYWVTEFHVDGFC
Sbjct: 481 THTAEGEAMQGIDISSYYHVNGVEDLEARNALNCNYPVVQQMVLDSLRYWVTEFHVDGFC 540

Query: 541 FVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWA 600
           F+NAS LLRG +GE LSRPP VEAIAFDPLLSK K++AD WDP+ L  KETRFPHWKRWA
Sbjct: 541 FINASSLLRGSNGECLSRPPLVEAIAFDPLLSKAKIIADCWDPRGLVPKETRFPHWKRWA 600

Query: 601 EVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVS 660
           EVN+KF +D+R+F RGEGL+  LATRLCG+GD+FSDGRGPAFSFNFI+RN GLPLVDLVS
Sbjct: 601 EVNTKFSNDVRNFLRGEGLLGDLATRLCGNGDIFSDGRGPAFSFNFISRNSGLPLVDLVS 660

Query: 661 FSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSG 720
           FS   LA+ELSWNC EEGPT+   VLE+RLKQIRNF+F+LFVSLGVPVLNMGDECGQS+G
Sbjct: 661 FSGVELAAELSWNCEEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECGQSTG 720

Query: 721 GSVAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQ 780
           GS A++D+++F+W+ L   F TQTTQFIAFLSSFR RR DL Q RNFLK ENIDW+ ++Q
Sbjct: 721 GSTAYSDRKAFDWNALGAGFATQTTQFIAFLSSFRIRRSDLLQKRNFLKEENIDWYGSDQ 780

Query: 781 SPPQWEDASCKFLAVMLRADKEE-NESITENPKTRSNIFMVFNASDQSESVALPEPLEGT 840
           S P+WED SCKFLA+ L+AD+EE NES  ++  +  ++F+ F+A+D SE+V LP   EG 
Sbjct: 781 SSPKWEDPSCKFLAMKLKADEEEANESGDDSSPSWGDLFVAFSAADLSETVILPPLPEGM 840

Query: 841 SWFRVVDTALPFPGFFSSDGELV--PMTGSVTYEIQAHSCALFEAKS 883
            WFR+VDTALPFPGFFS+DGE V     G   YE+++HSCALFEA+S
Sbjct: 841 GWFRLVDTALPFPGFFSTDGEPVAEQPAGLFAYEMKSHSCALFEARS 868