BLAST of CsaV3_7G028580 vs. NCBI nr
Match:
XP_004136188.1 (PREDICTED: isoamylase 2, chloroplastic [Cucumis sativus] >KGN44865.1 hypothetical protein Csa_7G392380 [Cucumis sativus])
HSP 1 Score: 1795.0 bits (4648), Expect = 0.0e+00
Identity = 885/885 (100.00%), Postives = 885/885 (100.00%), Query Frame = 0
Query: 1 MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA
Sbjct: 1 MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
Query: 61 VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS
Sbjct: 61 VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
Query: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
Query: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 886
RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 885
BLAST of CsaV3_7G028580 vs. NCBI nr
Match:
XP_008451396.1 (PREDICTED: isoamylase 2, chloroplastic [Cucumis melo])
HSP 1 Score: 1687.5 bits (4369), Expect = 0.0e+00
Identity = 830/884 (93.89%), Postives = 852/884 (96.38%), Query Frame = 0
Query: 1 MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
MATFLSSLVIQPFCV+NCGVTES KLA SDHLTYGQKTKYQ GKMDEARMLAHGE+KVGA
Sbjct: 1 MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
Query: 61 VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
VKSSHRNLSK Y SGI VGKSGQRLGIGGKSKEQR+VATYLFRTEFGDLVNVFVGK GS
Sbjct: 61 VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
FTVNIE+ SMQLVS DE LLLSW VYRSDSA VT NF+SSPPD TTGATETPFVKTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
KFSVELEFDAKHTPFYLSFVLK+PMGVDSGSSEIRSH+KTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
Query: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
SGDGS+NFSIFSS+AES+VLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGAS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300
Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
YGYQCKG KSHENQDGLEVSRIVVDPYAKILAPSIPKS QGLG P KFLGQIS VP FD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
Query: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
WDGEV PNLPMEKLFVYRLNV+RFT+DKSSQLPADIAGTFSGLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVI+EVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
TS +GALQGIDDSSYYF NRVANLEE+SALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
NASFLL+GHHGELLSRPPFVEAIAFDP+LSKTKLVADFWDPQEL SKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
NSNLASELSWNCGEEGPT+ LKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
VAFND+RSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQ+RNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
PQWEDASCKFLAVMLRADKEENESITENPKTRS+IFM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 885
RVVDTALPFPGFFSSDGELVPMTGSVTYE+QAHSCALFEAKSAN
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884
BLAST of CsaV3_7G028580 vs. NCBI nr
Match:
XP_022953509.1 (isoamylase 2, chloroplastic isoform X1 [Cucurbita moschata] >XP_022953510.1 isoamylase 2, chloroplastic isoform X1 [Cucurbita moschata])
HSP 1 Score: 1510.4 bits (3909), Expect = 0.0e+00
Identity = 736/885 (83.16%), Postives = 803/885 (90.73%), Query Frame = 0
Query: 1 MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
MATFLSSL IQPFC YN G TES KLA SD+ TYGQK YQ GK+DEARM AHGE+KVG
Sbjct: 1 MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60
Query: 61 VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
KS HRN+ + YA S ISVGKSGQ+ G GGK++E R+VATYLFRTEFGDLV FVGK+GS
Sbjct: 61 TKSLHRNVCRAYAPSRISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120
Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
F +IE+ S++L SIDE LLLSWGVYRS+SA+VTPNF+S P+ET GATETPFV+TSEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180
Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
KF+VELEFDAKH PFYLSF+LKYP+GVDSGSSEI+SH+KTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240
Query: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
SGDGS+NFSIFSS+AES+VLCLYNDSTSEKP+LELDLDPYINRSGNIWHASFEGAS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300
Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
YGY+C+GSK HENQD EVSRI+VDPYAKIL SIPK+ GQGLGLPSKFLGQI VP F
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360
Query: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
WDGEV PNLPMEKLF+YRLNV+ FT+DKSSQLP D AGTFSGLT+KLLHFK LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420
Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS++NS+KEMVKELHANGVEVILEVV+TH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480
Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
T+ +GALQGIDDS YYF NRVAN E KSALNCN+PIVQQLLLDSLR+WVTEFH+DGFCF+
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540
Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
NAS LL+GHHGELLSRPPFVEAIAFDPLLSKTK++ADFWDPQE KETRFPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600
Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
NSKF +DI++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
+S LASELSWNCGEEGPTSN++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
VAF D++SFNWD LKTDFGTQTTQFI FLSSFRSRRFDLFQ+RNFLK ENIDWFDNNQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780
Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
P WED SCKFLAVMLRADKEE+ SITENPK +S+IFM FNASDQS SVALPEP EGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840
Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 886
R+VDTAL FPGFFSSDGE VP T VTY +QAHSC LFEAKS ND
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFND 884
BLAST of CsaV3_7G028580 vs. NCBI nr
Match:
XP_023548355.1 (isoamylase 2, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023548356.1 isoamylase 2, chloroplastic [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1510.4 bits (3909), Expect = 0.0e+00
Identity = 734/885 (82.94%), Postives = 801/885 (90.51%), Query Frame = 0
Query: 1 MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
MATFLSSL IQPFC YN G TES KLA +D+ TYGQK YQ GK+DEARM AHGE+KVG
Sbjct: 1 MATFLSSL-IQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60
Query: 61 VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
KS HRN+ + YA SGISVGKSGQ+ G GGK++E R+VATYLFRTEFGDLV FVGK+GS
Sbjct: 61 TKSLHRNVCRAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120
Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
F VNIE+ S++L SIDE LLLSWGVYRS+SA+VTPNF+S P+ET GATE PFV+TSEG
Sbjct: 121 GFMVNIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATEAPFVRTSEG 180
Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
KF+VELEFDAKH PFYLSF+LKYP+GVDSGSSEI+SH+KTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240
Query: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
SGDGS+NFSIFSS+AES+VLCLYNDSTSEKP+LELDLDPYINRSGNIWHASFEGAS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300
Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
YGY+C+GSK HENQD EVSRI+VDPYAKIL SIPK+ GQGLGLPSKFLGQI VP F
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360
Query: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
WDGEV PNLPMEKLF+YRLNV+ FT+DKSSQLP DIAGTFSGLT+KLLHFK LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLL 420
Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS++NS+KEMVK LHANGVEVILEVV+TH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKVLHANGVEVILEVVFTH 480
Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
T+ +GALQGIDDS YYF NRV N E KSALNCN+PIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVTNSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFI 540
Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
NAS LL+GHHGELLSRPPF EAIAFDPLLSKTK++ADFWDPQE KE RFPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFAEAIAFDPLLSKTKIIADFWDPQESVPKEIRFPHWKRWAEL 600
Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
NSKF +DI++FFRGEGL+ SLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVRSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
+S LASELSWNCGEEGPTSN++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
VAF D++SFNWD LKTDFGTQTTQFI FLSSFRSRRFDLFQ+RNFLK ENIDWFDNNQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780
Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
P WED SCKFLAVMLRADKEE+ SITENPK +S+IFM FNASDQS SVALPEP EGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTTWF 840
Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 886
R+VDTAL FPGFFSSDGE VP T +TYE+QAHSC LFEAKS ND
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPITYEMQAHSCTLFEAKSFND 884
BLAST of CsaV3_7G028580 vs. NCBI nr
Match:
XP_022953511.1 (isoamylase 2, chloroplastic isoform X2 [Cucurbita moschata])
HSP 1 Score: 1489.6 bits (3855), Expect = 0.0e+00
Identity = 730/885 (82.49%), Postives = 795/885 (89.83%), Query Frame = 0
Query: 1 MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
MATFLSSL IQPFC YN G TES KLA SD+ TYGQK YQ GK+DEARM AHGE+KVG
Sbjct: 1 MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60
Query: 61 VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
KS HRN+ + YA S ISV GKS+E R+VATYLFRTEFGDLV FVGK+GS
Sbjct: 61 TKSLHRNVCRAYAPSRISV----------GKSEEDRKVATYLFRTEFGDLVYAFVGKRGS 120
Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
F +IE+ S++L SIDE LLLSWGVYRS+SA+VTPNF+S P+ET GATETPFV+TSEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180
Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
KF+VELEFDAKH PFYLSF+LKYP+GVDSGSSEI+SH+KTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240
Query: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
SGDGS+NFSIFSS+AES+VLCLYNDSTSEKP+LELDLDPYINRSGNIWHASFEGAS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300
Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
YGY+C+GSK HENQD EVSRI+VDPYAKIL SIPK+ GQGLGLPSKFLGQI VP F
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360
Query: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
WDGEV PNLPMEKLF+YRLNV+ FT+DKSSQLP D AGTFSGLT+KLLHFK LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420
Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS++NS+KEMVKELHANGVEVILEVV+TH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480
Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
T+ +GALQGIDDS YYF NRVAN E KSALNCN+PIVQQLLLDSLR+WVTEFH+DGFCF+
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540
Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
NAS LL+GHHGELLSRPPFVEAIAFDPLLSKTK++ADFWDPQE KETRFPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600
Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
NSKF +DI++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
+S LASELSWNCGEEGPTSN++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
VAF D++SFNWD LKTDFGTQTTQFI FLSSFRSRRFDLFQ+RNFLK ENIDWFDNNQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780
Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
P WED SCKFLAVMLRADKEE+ SITENPK +S+IFM FNASDQS SVALPEP EGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840
Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 886
R+VDTAL FPGFFSSDGE VP T VTY +QAHSC LFEAKS ND
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFND 874
BLAST of CsaV3_7G028580 vs. TAIR10
Match:
AT1G03310.2 (debranching enzyme 1)
HSP 1 Score: 942.6 bits (2435), Expect = 1.8e-274
Identity = 477/881 (54.14%), Postives = 628/881 (71.28%), Query Frame = 0
Query: 14 CVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGAVKSSHRNLSKTYA 73
C N G+T + K S L G+K K + G E M V S+ ++Y
Sbjct: 13 CCLNNGITRTWKF-PSARLFTGRKNKIKLG--SETLMFTRKRFMGDLVTSA----LQSYQ 72
Query: 74 KSGISVGKSGQRLGIGGKSK-----EQRRVATYLFRTEFGDLVNVFVGKKGSTFTVNIEV 133
S I K+ L S+ + ++V +Y FRT+ G LV V V KK +++ + V
Sbjct: 73 FSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYV 132
Query: 134 PSMQLVSIDEA-LLLSWGVYRSDSALVTP-NFESSPPDETTGATETPFVKTSEGKFSVEL 193
S++L D++ L++ WGVYRSDS+ P +FE+S D T TET FVK+S + + L
Sbjct: 133 SSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGL 192
Query: 194 EFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSIS-GDGS 253
EFD K +PFYLSF LK G D E+ +H+ T F +PVGF G+P PLGLS D S
Sbjct: 193 EFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDS 252
Query: 254 VNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVSYGYQC 313
NFS FS S+ ++VLCLY+DST++KP LELDLDPY+NR+G++WHAS + FV YGY+C
Sbjct: 253 WNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRC 312
Query: 314 KGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFDWDGEV 373
K + + +E IV+DPYA ++ S+ + K+LG +SK P+FDW +V
Sbjct: 313 KETAHSKEDVDVEGEPIVLDPYATVVGKSVSQ----------KYLGSLSKSPSFDWGEDV 372
Query: 374 HPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQ 433
PN+P+EKL VYRLNV+ FT +SS+LP+++AGTFSG+ +K+ H K LG NAVLLEPIF
Sbjct: 373 SPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFS 432
Query: 434 FDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTHTSGNG 493
F E++GPYFPFHFFSP + YGPS + SA+NSMK MVK+LH+ G+EV+LEVV+THT+ +G
Sbjct: 433 FSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG 492
Query: 494 ALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFL 553
AL+GIDDSSYY+ R +L+ KS LNCNYP+VQQL+L+SLRYWVTEFHVDGFCF+NAS L
Sbjct: 493 ALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSL 552
Query: 554 LRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFC 613
LRG HGE LSRPP VEAIAFDPLL++TKL+AD WDP E+ KE RFPHWKRWAE+N+++C
Sbjct: 553 LRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYC 612
Query: 614 SDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSNSNLA 673
++R+F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I+RN GL LVD+VSFS LA
Sbjct: 613 RNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELA 672
Query: 674 SELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFND 733
SELSWNCGEEG T+ VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS
Sbjct: 673 SELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLES 732
Query: 734 KRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSPPQWED 793
++ F+W+LL + FGTQ TQFI+F++S R+RR D+FQ R+FLK ENI W+ N+Q+ P+WED
Sbjct: 733 RKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWED 792
Query: 794 ASCKFLAVMLRADKEENE--SITENPKTRSN-IFMVFNASDQSESVALPEPLEGTSWFRV 853
+ KFLA+ ++++ EE E S+ E + +SN +F+ FNASD ESV LP +G+ W R+
Sbjct: 793 PASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRL 852
Query: 854 VDTALPFPGFFSSDGELV----PMTGSVTYEIQAHSCALFE 880
VDTALPFPGFFS +GE V P+ V YE++ +SC LFE
Sbjct: 853 VDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876
BLAST of CsaV3_7G028580 vs. TAIR10
Match:
AT4G09020.1 (isoamylase 3)
HSP 1 Score: 374.0 bits (959), Expect = 2.5e-103
Identity = 246/701 (35.09%), Postives = 360/701 (51.36%), Query Frame = 0
Query: 231 GYPSPLGLSISGDGSVNFSIFSSSAESLVLCLY-----NDSTSEKPLLELDLDPYINRSG 290
G SPLG+S D +NF++FS +A S+ LCL D T + ++EL LDP +N++G
Sbjct: 88 GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147
Query: 291 NIWHASFEGAS-KFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLG 350
+ WH E V YGY+ G + + S +++DPYAK++ S G
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGH--SSFGDSSQ 207
Query: 351 LPSKFLGQIS-KVPTFDW-DGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSG 410
++F G + FDW D PN+P + L +Y +NV FT D+SS + I G++ G
Sbjct: 208 KFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267
Query: 411 LTKKLLHFKNLGVNAVLLEPIFQFDEKE--------------GPYFPFHFFSPTNNYGP- 470
+K+ H ++LG+NAV L P+F+FDE E Y +FF+P + Y
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327
Query: 471 SGASISAINSMKEMVKELHANGVEVILEVVYTHTSG-------NGALQGIDDSSYYF--- 530
G I A KEMVK LH+ G+EVIL+VVY HT+ + +GID+ YY
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387
Query: 531 TNRVANLEE-KSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLRGHHGELLSR 590
N++ N + LNCN+P+V +L+LDSLR+WVTE+HVDGF F AS L R G LS
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447
Query: 591 PPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFCSDIRDFFRGE- 650
PP + AIA D +LS+ K++A+ WD L +FP+W RWAE N + D+R F +G+
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWDCGGL-YLVGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507
Query: 651 GLISSLATRLCGSGDVFS-DGRGPAFSFNFIARNVGLPLVDLVSFS-----------NSN 710
G+ S ATR+ GS D++ + R P NF+ + G L DLVS++ N
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567
Query: 711 LASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSS-GGSVA 770
SWNCG EG T + + R +Q++NF L +S G P++ MGDE G + G + +
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNS 627
Query: 771 FNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRR-FDLFQNRNFLKGENIDWFDNNQSPP 830
+ S N K + F F + R + ++ NFL I W ++N
Sbjct: 628 YGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWHEDN---- 687
Query: 831 QWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWFR 882
W+++ KFLA L ++ I +I++ FNA D +P+P G WFR
Sbjct: 688 -WDNSESKFLAFTL------HDGIGGR-----DIYVAFNAHDYFVKALIPQPPPGKQWFR 747
BLAST of CsaV3_7G028580 vs. TAIR10
Match:
AT2G39930.1 (isoamylase 1)
HSP 1 Score: 352.1 bits (902), Expect = 1.0e-96
Identity = 242/690 (35.07%), Postives = 345/690 (50.00%), Query Frame = 0
Query: 231 GYPSPLGLSISGDGSVNFSIFSSSAESLVLCL--YNDSTSEKPLLELDLDPYINRSGNIW 290
G PSP G ++ DG VNFS++S+++ S +CL +D K E+ LDP NR+G++W
Sbjct: 72 GLPSPFGPTVRDDG-VNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVW 131
Query: 291 HASFEGASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSK 350
H G K + YGY+ G S E + S I++DPYAK + I + LG
Sbjct: 132 HVFLRGDFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAI---ISRDEFGVLGPDDN 191
Query: 351 FLGQIS-KVPT----FDWDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGL 410
Q++ VPT FDW+G++H LP + L +Y ++V FT +SS++ + GT+ G+
Sbjct: 192 CWPQMACMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQGV 251
Query: 411 TKKLLHFKNLGVNAVLLEPIFQFDEKE---------------GPYFPFHFFSPTNNYGPS 470
+KL H K LG+N + L P +F+E E Y FFSP Y +
Sbjct: 252 AEKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASA 311
Query: 471 GASI---SAINSMKEMVKELHANGVEVILEVVYTHTS-GNG-----ALQGIDDSSYYFTN 530
++ AIN K +VKE H G+EVI++VV HT+ GN + +G+D+S YY
Sbjct: 312 SSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLA 371
Query: 531 RVANLEEKS----ALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLRGH------ 590
S NCN+P+V+Q +LD LRYWVTE HVDGF F S + R
Sbjct: 372 PKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAA 431
Query: 591 -------HGELL------SRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKR 650
G+LL S PP ++ I+ DP+L KL+A+ WD L + FPHW
Sbjct: 432 NVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGL-YQVGMFPHWGI 491
Query: 651 WAEVNSKFCSDIRDFFRG-EGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVD 710
W+E N KF +R F +G +G + A LCGS +++ GR P S NFI + G L D
Sbjct: 492 WSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLAD 551
Query: 711 LVSFSN-SNLAS----------ELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGV 770
LV+++N +NLA+ SWNCGEEG +++ V R +Q+RNF L VS GV
Sbjct: 552 LVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGV 611
Query: 771 PVLNMGDECGQSSGGS----VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLF 830
P++ MGDE G + GG+ N F WD K + + +F L FR L
Sbjct: 612 PMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWD-KKEEAHSDFFRFCRILIKFRDECESLG 671
Query: 831 QNRNFLKGENIDWFDNNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFN 851
N +F + + W P W + S +F+A L D + E I++ FN
Sbjct: 672 LN-DFPTAKRLQWHGLAPEIPNWSETS-RFVAFSL-VDSVKKE-----------IYVAFN 731
BLAST of CsaV3_7G028580 vs. TAIR10
Match:
AT5G04360.1 (limit dextrinase)
HSP 1 Score: 69.7 bits (169), Expect = 1.0e-11
Identity = 86/366 (23.50%), Postives = 144/366 (39.34%), Query Frame = 0
Query: 232 YPSPLGLSISGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHAS 291
Y PLG + +G V+ +++ +A+++ +C+Y + + P +++ P + + +W S
Sbjct: 204 YDGPLGAHFTPEG-VSLHLWAPTAQAVSVCIYKNPLDKSP---MEICP-LKEANGVW--S 263
Query: 292 FEGASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLG 351
EGA + Y K S H + LE DPYA+ L+ K+ FL
Sbjct: 264 TEGACSWGGCYYVYKVSVYHPSTMKLETC-YANDPYARGLSADGRKT----------FLV 323
Query: 352 QISK--VPTFDWD--GEVHPNL-PMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKK 411
+ + WD + P L + +Y L+V F+ + + P + G + +K
Sbjct: 324 NLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPENRGGYLAFTSKD 383
Query: 412 LLHFKNL------GVNAVLLEPIFQF----DEKEG------------------------- 471
K+L G+ + L P FQF DEKE
Sbjct: 384 SAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQARITE 443
Query: 472 ---------PYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTHTS 531
Y P + P +Y I ++MV+ L+ G+ V+L+VVY H
Sbjct: 444 IQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRIIEFRKMVQALNCTGLNVVLDVVYNHLH 503
Query: 532 GNG------ALQGIDDSSYYFTNRVANLEEKSALN---CNYPIVQQLLLDSLRYWVTEFH 540
+G L I Y N +E + +N + +V +L+ D L WV +
Sbjct: 504 ASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIRDDLLNWVVNYK 551
BLAST of CsaV3_7G028580 vs. TAIR10
Match:
AT3G20440.2 (Alpha amylase family protein)
HSP 1 Score: 54.7 bits (130), Expect = 3.4e-07
Identity = 42/161 (26.09%), Postives = 78/161 (48.45%), Query Frame = 0
Query: 399 TFSGLTKKLL-HFKNLGVNAVLLEPIFQF-DEKEGPYFPFHFFSPTNNYGPSGASISAIN 458
TF TKK+L H K G NA+ L + + D Y +FF+ ++ YG +
Sbjct: 416 TFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDYFTVGYRVTNFFAASSRYGTP-------D 475
Query: 459 SMKEMVKELHANGVEVILEVVYTHTS-----GNGALQGIDDSSYYFTNRVANLEEKSALN 518
K +V E H G+ V L++V+++ + G G +D +++ R + + +
Sbjct: 476 DFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFDGSNDCYFHYGKRGHHKHWGTRMF 535
Query: 519 CNYPI-VQQLLLDSLRYWVTEFHVDGFCFVNASFLLRGHHG 552
+ V L+ +L +W+TE+ VDG+ F + + ++ H+G
Sbjct: 536 KYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIYTHNG 569
BLAST of CsaV3_7G028580 vs. Swiss-Prot
Match:
sp|Q8L735|ISOA2_ARATH (Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2)
HSP 1 Score: 942.6 bits (2435), Expect = 3.2e-273
Identity = 477/881 (54.14%), Postives = 628/881 (71.28%), Query Frame = 0
Query: 14 CVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGAVKSSHRNLSKTYA 73
C N G+T + K S L G+K K + G E M V S+ ++Y
Sbjct: 13 CCLNNGITRTWKF-PSARLFTGRKNKIKLG--SETLMFTRKRFMGDLVTSA----LQSYQ 72
Query: 74 KSGISVGKSGQRLGIGGKSK-----EQRRVATYLFRTEFGDLVNVFVGKKGSTFTVNIEV 133
S I K+ L S+ + ++V +Y FRT+ G LV V V KK +++ + V
Sbjct: 73 FSKICASKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYV 132
Query: 134 PSMQLVSIDEA-LLLSWGVYRSDSALVTP-NFESSPPDETTGATETPFVKTSEGKFSVEL 193
S++L D++ L++ WGVYRSDS+ P +FE+S D T TET FVK+S + + L
Sbjct: 133 SSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGL 192
Query: 194 EFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSIS-GDGS 253
EFD K +PFYLSF LK G D E+ +H+ T F +PVGF G+P PLGLS D S
Sbjct: 193 EFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDS 252
Query: 254 VNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVSYGYQC 313
NFS FS S+ ++VLCLY+DST++KP LELDLDPY+NR+G++WHAS + FV YGY+C
Sbjct: 253 WNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRC 312
Query: 314 KGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFDWDGEV 373
K + + +E IV+DPYA ++ S+ + K+LG +SK P+FDW +V
Sbjct: 313 KETAHSKEDVDVEGEPIVLDPYATVVGKSVSQ----------KYLGSLSKSPSFDWGEDV 372
Query: 374 HPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQ 433
PN+P+EKL VYRLNV+ FT +SS+LP+++AGTFSG+ +K+ H K LG NAVLLEPIF
Sbjct: 373 SPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFS 432
Query: 434 FDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTHTSGNG 493
F E++GPYFPFHFFSP + YGPS + SA+NSMK MVK+LH+ G+EV+LEVV+THT+ +G
Sbjct: 433 FSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG 492
Query: 494 ALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFL 553
AL+GIDDSSYY+ R +L+ KS LNCNYP+VQQL+L+SLRYWVTEFHVDGFCF+NAS L
Sbjct: 493 ALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSL 552
Query: 554 LRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFC 613
LRG HGE LSRPP VEAIAFDPLL++TKL+AD WDP E+ KE RFPHWKRWAE+N+++C
Sbjct: 553 LRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYC 612
Query: 614 SDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSNSNLA 673
++R+F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I+RN GL LVD+VSFS LA
Sbjct: 613 RNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELA 672
Query: 674 SELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFND 733
SELSWNCGEEG T+ VL++RLKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS
Sbjct: 673 SELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLES 732
Query: 734 KRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSPPQWED 793
++ F+W+LL + FGTQ TQFI+F++S R+RR D+FQ R+FLK ENI W+ N+Q+ P+WED
Sbjct: 733 RKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWED 792
Query: 794 ASCKFLAVMLRADKEENE--SITENPKTRSN-IFMVFNASDQSESVALPEPLEGTSWFRV 853
+ KFLA+ ++++ EE E S+ E + +SN +F+ FNASD ESV LP +G+ W R+
Sbjct: 793 PASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRL 852
Query: 854 VDTALPFPGFFSSDGELV----PMTGSVTYEIQAHSCALFE 880
VDTALPFPGFFS +GE V P+ V YE++ +SC LFE
Sbjct: 853 VDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876
BLAST of CsaV3_7G028580 vs. Swiss-Prot
Match:
sp|Q6AU80|ISOA2_ORYSJ (Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=1 SV=1)
HSP 1 Score: 699.5 bits (1804), Expect = 4.7e-200
Identity = 350/716 (48.88%), Postives = 473/716 (66.06%), Query Frame = 0
Query: 183 SVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPV----GFGRGYPSPLGL 242
+ EL FD PFYLSF+L D+ +EIR+H+KTSF VPV
Sbjct: 105 AAELSFDPTRAPFYLSFLL-----TDASGAEIRTHRKTSFRVPVXXXXXXXXXXXXXXXX 164
Query: 243 SISGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKF 302
LE+DLDPYI+R+GN+WH S +
Sbjct: 165 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXALEIDLDPYIHRTGNVWHVSLASVDGY 224
Query: 303 VSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSS--GQGLGLPS-KFLGQISK 362
VSY + C G + R ++DPYAK++ + +S +G+ PS + ++
Sbjct: 225 VSYAFCCGG-----------IRRPLLDPYAKVIGDFVSSNSVYDEGVTAPSMRCFASLAI 284
Query: 363 VPTFDWDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGV 422
P+++W + HP LP+EKL VYR NV FT D+SS LP D AGTF+GL+ K+ HF++LGV
Sbjct: 285 APSYNWGRDRHPRLPLEKLVVYRANVALFTKDRSSGLPDDAAGTFTGLSAKVEHFRSLGV 344
Query: 423 NAVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILE 482
NA+LLEP+F F + +GPYFP+HFFSP N Y G S+SAI SMK+MV+ +H NG+EV+LE
Sbjct: 345 NAILLEPVFPFHQVKGPYFPYHFFSPMNLYSSKGLSVSAIKSMKDMVRVMHRNGIEVLLE 404
Query: 483 VVYTHTSGNG------ALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWV 542
VV+THT+ +++GID+SSYY N +A + S LNCN+P+ Q+L+LDSLR+WV
Sbjct: 405 VVFTHTAEGESECQTISMRGIDNSSYYIANGIAGC-KASILNCNHPVTQKLILDSLRHWV 464
Query: 543 TEFHVDGFCFVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKET 602
+FHVDGFCF+NA FL+RG GE LSRPP +EAI FDP+LS TK++AD W P ++ + +
Sbjct: 465 LDFHVDGFCFINAPFLVRGPGGEYLSRPPLLEAITFDPVLSMTKIIADPWSPLDISNVQF 524
Query: 603 RFPHWKRWAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNV 662
FPHWKRWAEVN++F D+R F + E LIS LATRLCGSGD+FS RGPAFSFN ++RN
Sbjct: 525 PFPHWKRWAEVNTRFSIDVRKFLKREALISDLATRLCGSGDLFST-RGPAFSFNHVSRNS 584
Query: 663 GLPLVDLVSFSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNM 722
GL LVDLVSFSN +L SE SWNCGEEGP+ N VL+ RL+QIRNF+F+LFVSLGVPVLNM
Sbjct: 585 GLSLVDLVSFSNDDLLSESSWNCGEEGPSENSAVLQTRLRQIRNFLFILFVSLGVPVLNM 644
Query: 723 GDECGQSSGGSVAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGE 782
GDECG S+ GSV++ D+ NW +KT F + T FI+FL++ RSRR D+FQ R FLK E
Sbjct: 645 GDECGHSAAGSVSYKDRGPLNWRGMKTTFVKEVTGFISFLTALRSRRGDIFQRREFLKLE 704
Query: 783 NIDWFDNNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVA 842
NI W+ ++ P W+D + FL + + A+ +E + R ++++ FNA+++S S A
Sbjct: 705 NIHWYGSDLCEPGWDDPTSNFLCMHINAEVDEMAA----DSVRGDLYICFNANEESVSAA 764
Query: 843 LPEPLEGTSWFRVVDTALPFPGFFS--SDGELVPMTGSVTYEIQAHSCALFEAKSA 884
LP EG+ W R+VDT+L FPGFF+ S+ ++ + G +Y ++AH+C LFE+KSA
Sbjct: 765 LPALAEGSVWLRLVDTSLAFPGFFATESNPKVQQVPGLSSYHVEAHTCVLFESKSA 798
BLAST of CsaV3_7G028580 vs. Swiss-Prot
Match:
sp|Q9M0S5|ISOA3_ARATH (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)
HSP 1 Score: 374.0 bits (959), Expect = 4.6e-102
Identity = 246/701 (35.09%), Postives = 360/701 (51.36%), Query Frame = 0
Query: 231 GYPSPLGLSISGDGSVNFSIFSSSAESLVLCLY-----NDSTSEKPLLELDLDPYINRSG 290
G SPLG+S D +NF++FS +A S+ LCL D T + ++EL LDP +N++G
Sbjct: 88 GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147
Query: 291 NIWHASFEGAS-KFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLG 350
+ WH E V YGY+ G + + S +++DPYAK++ S G
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGH--SSFGDSSQ 207
Query: 351 LPSKFLGQIS-KVPTFDW-DGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSG 410
++F G + FDW D PN+P + L +Y +NV FT D+SS + I G++ G
Sbjct: 208 KFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267
Query: 411 LTKKLLHFKNLGVNAVLLEPIFQFDEKE--------------GPYFPFHFFSPTNNYGP- 470
+K+ H ++LG+NAV L P+F+FDE E Y +FF+P + Y
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327
Query: 471 SGASISAINSMKEMVKELHANGVEVILEVVYTHTSG-------NGALQGIDDSSYYF--- 530
G I A KEMVK LH+ G+EVIL+VVY HT+ + +GID+ YY
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387
Query: 531 TNRVANLEE-KSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLRGHHGELLSR 590
N++ N + LNCN+P+V +L+LDSLR+WVTE+HVDGF F AS L R G LS
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447
Query: 591 PPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKFCSDIRDFFRGE- 650
PP + AIA D +LS+ K++A+ WD L +FP+W RWAE N + D+R F +G+
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWDCGGL-YLVGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507
Query: 651 GLISSLATRLCGSGDVFS-DGRGPAFSFNFIARNVGLPLVDLVSFS-----------NSN 710
G+ S ATR+ GS D++ + R P NF+ + G L DLVS++ N
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567
Query: 711 LASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSS-GGSVA 770
SWNCG EG T + + R +Q++NF L +S G P++ MGDE G + G + +
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNS 627
Query: 771 FNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRR-FDLFQNRNFLKGENIDWFDNNQSPP 830
+ S N K + F F + R + ++ NFL I W ++N
Sbjct: 628 YGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWHEDN---- 687
Query: 831 QWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWFR 882
W+++ KFLA L ++ I +I++ FNA D +P+P G WFR
Sbjct: 688 -WDNSESKFLAFTL------HDGIGGR-----DIYVAFNAHDYFVKALIPQPPPGKQWFR 747
BLAST of CsaV3_7G028580 vs. Swiss-Prot
Match:
sp|B9G434|ISOA3_ORYSJ (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)
HSP 1 Score: 368.2 bits (944), Expect = 2.5e-100
Identity = 247/723 (34.16%), Postives = 368/723 (50.90%), Query Frame = 0
Query: 212 SEIRSHKKTSFSVPVGFGRGYPSPLGLSISGDGSVNFSIFSSSAESLVLCLY-----NDS 271
+E R + +P + G PLG+S +G +NF++FS A S++LCL +
Sbjct: 84 TEERGCTMSDTEMPFKYSSGKAFPLGVS-QVEGGLNFALFSQHASSVILCLKLPGRGTED 143
Query: 272 TSEKPLLELDLDPYINRSGNIWHASFEG-ASKFVSYGYQCKGSKSHENQDGLEVSRIVVD 331
++E LD N++G+IWH EG + V YGY+ G + + + S +++D
Sbjct: 144 EKGADVVEFVLDQQKNKTGDIWHVIVEGLPASGVLYGYRVGGPQGWDQGHRFDSSTVLLD 203
Query: 332 PYAKILAPSIPKSSGQGLGLPSKFLGQIS-KVPTFDWDGEVH-PNLPMEKLFVYRLNVER 391
PYAK++ S K G S+ G FDW + PNLP L +Y +NV
Sbjct: 204 PYAKLV--SGRKYFGVAEEKSSQHFGTYDFDSSPFDWGDDYRLPNLPEADLVIYEMNVRA 263
Query: 392 FTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQFDEKEGPYFP-------- 451
FT D+SS L + G++ GL K+ H LGVNAV L P+F++DE E +P
Sbjct: 264 FTADESSGLDSTSRGSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNPRDHMVN 323
Query: 452 ------FHFFSPTNNYGPSGAS-ISAINSMKEMVKELHANGVEVILEVVYTHTSGNG--- 511
+FF+P + Y +G ++A +K+MVKELH G+EVIL+VVY HT+
Sbjct: 324 TWGYSTINFFAPMSRYASAGGGPVAASKELKQMVKELHKAGIEVILDVVYNHTNEADDAH 383
Query: 512 ----ALQGIDDSSYYFTNRVANLEE------KSALNCNYPIVQQLLLDSLRYWVTEFHVD 571
+ +GID+ YY + N E + LNCN+P+V++L+LDSLR+WV E+H+D
Sbjct: 384 PYMTSFRGIDNKVYYMLDLNKNAELLNFSGCGNTLNCNHPVVKELILDSLRHWVEEYHID 443
Query: 572 GFCFVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWK 631
GF F AS L RG G L PP ++ IA D +LS+ K++A+ WD L RFP+W
Sbjct: 444 GFRFDLASVLCRGPDGCPLDAPPLIKEIAKDAVLSRCKIIAEPWDCGGL-YLVGRFPNWD 503
Query: 632 RWAEVNSKFCSDIRDFFRGE-GLISSLATRLCGSGDVFS-DGRGPAFSFNFIARNVGLPL 691
RWAE N K+ D+R F +G+ G+ ATR+ GS D++ + R P NF+ + G L
Sbjct: 504 RWAEWNGKYRDDLRRFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNFVIAHDGFTL 563
Query: 692 VDLVSFSNSN-----------LASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSL 751
DLVS++ + SWNCG EG T++L VL R +Q++NF L +S
Sbjct: 564 CDLVSYNLKHNDANGEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKNFHVALMISQ 623
Query: 752 GVPVLNMGDECGQSS-GGSVAFNDK---RSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFD 811
G P++ MGDE G + G + ++ +F W+ L+ +F + + FR
Sbjct: 624 GTPMMLMGDEYGHTRYGNNNSYGHDTCINNFQWEQLEQRRDGH-FRFFSEMIKFRHSNPI 683
Query: 812 LFQNRNFLKGENIDWFDNNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMV 871
L ++R FL ++ W ++ WE+ KFLA + + + +I++
Sbjct: 684 LRRDR-FLNKNDVTWHED-----CWENQESKFLAFTV-----------HDHNSGGDIYLA 743
Query: 872 FNASDQSESVALPEPLEGTSWFRVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALF 882
FNA D +P P W RVVDT L P +G VP TG Y I +S L
Sbjct: 744 FNAHDYFVDAVIPPPPHHKCWNRVVDTNLESPNDIVPEG--VPFTGP-KYRIAPYSSILL 781
BLAST of CsaV3_7G028580 vs. Swiss-Prot
Match:
sp|D0TZF0|ISOA1_ORYSJ (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)
HSP 1 Score: 352.4 bits (903), Expect = 1.4e-95
Identity = 234/689 (33.96%), Postives = 343/689 (49.78%), Query Frame = 0
Query: 231 GYPSPLGLSISGDGSVNFSIFSSSAESLVLCLY--NDSTSEKPLLELDLDPYINRSGNIW 290
G P+PLG + + DG VNF+++S+ A + LCL+ +D +++ E+ LDP NR+GN+W
Sbjct: 99 GMPAPLG-ATALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLDPLFNRTGNVW 158
Query: 291 HASFEGASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAK-ILAPSIPKSSGQGLGLPS 350
H EG + YGY+ G + +VS +VVDPYAK +++ G G
Sbjct: 159 HVFIEGELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVISRGEYGVPGPGGDCWP 218
Query: 351 KFLGQIS-KVPTFDWDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKK 410
+ G I TFDW G++ P + L +Y +++ FT SS + + GT+ G K
Sbjct: 219 QMAGMIPLPYSTFDWQGDLPLRYPQKDLVIYEMHLRGFTKHSSSNV--EHPGTYIGAISK 278
Query: 411 LLHFKNLGVNAVLLEPIFQFDEKE----------GPYFPFHFFSPTNNYGPSG---ASIS 470
L + K LGVN V L P +F+E E Y +FFSP Y G
Sbjct: 279 LDYLKELGVNCVELMPCHEFNELEYFSCSSKMNFWGYSTINFFSPMIRYSSGGIRNCGRD 338
Query: 471 AINSMKEMVKELHANGVEVILEVVYTHTS-GNG-----ALQGIDDSSYYFTNRVANLEEK 530
AIN K V+E H G+EVI++VV+ HT+ GN + +GID+S+YY
Sbjct: 339 AINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSTYYMLAPKGEFYNY 398
Query: 531 S----ALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNASFLLRG--------------- 590
S NCN+P+V++ ++D LRYWVTE HVDGF F AS + RG
Sbjct: 399 SGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYGSPVE 458
Query: 591 ----HHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSKF 650
G L+ PP ++ I+ DP+L KL+A+ WD L + +FPHWK W+E N K+
Sbjct: 459 GDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWDAGGL-YQVGQFPHWKIWSEWNGKY 518
Query: 651 CSDIRDFFRG-EGLISSLATRLCGSGDVF-SDGRGPAFSFNFIARNVGLPLVDLVSFSNS 710
+R F +G +G A LCGS ++ + GR P S NF+ + G L DLV+++
Sbjct: 519 RDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYNKK 578
Query: 711 NLAS-----------ELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGD 770
+S LSWNCGEEG + L V R +Q+RNF L VS GVP+ MGD
Sbjct: 579 YNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYMGD 638
Query: 771 ECGQSSGG--SVAFNDK--RSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLK 830
E G + GG + +D F WD K + + +F + ++ FR ++ + +F
Sbjct: 639 EYGHTKGGNNNTYCHDHYVNYFRWD--KKEESSDLQRFCSLMTKFR-KQCESLGLADFPT 698
Query: 831 GENIDWFDNNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSES 857
+ + W + P W + S +F+A + +T+ I++ FNAS
Sbjct: 699 AQRLHWHGHQPGKPDWSETS-RFVA------------FSTKDETKGEIYVAFNASHLPAV 758
BLAST of CsaV3_7G028580 vs. TrEMBL
Match:
tr|A0A0A0K551|A0A0A0K551_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 SV=1)
HSP 1 Score: 1795.0 bits (4648), Expect = 0.0e+00
Identity = 885/885 (100.00%), Postives = 885/885 (100.00%), Query Frame = 0
Query: 1 MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA
Sbjct: 1 MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
Query: 61 VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS
Sbjct: 61 VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
Query: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
Query: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 886
RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 885
BLAST of CsaV3_7G028580 vs. TrEMBL
Match:
tr|A0A1S3BRF8|A0A1S3BRF8_CUCME (isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1)
HSP 1 Score: 1687.5 bits (4369), Expect = 0.0e+00
Identity = 830/884 (93.89%), Postives = 852/884 (96.38%), Query Frame = 0
Query: 1 MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
MATFLSSLVIQPFCV+NCGVTES KLA SDHLTYGQKTKYQ GKMDEARMLAHGE+KVGA
Sbjct: 1 MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60
Query: 61 VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120
VKSSHRNLSK Y SGI VGKSGQRLGIGGKSKEQR+VATYLFRTEFGDLVNVFVGK GS
Sbjct: 61 VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120
Query: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180
FTVNIE+ SMQLVS DE LLLSW VYRSDSA VT NF+SSPPD TTGATETPFVKTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180
Query: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240
KFSVELEFDAKHTPFYLSFVLK+PMGVDSGSSEIRSH+KTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240
Query: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300
SGDGS+NFSIFSS+AES+VLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGAS FVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300
Query: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360
YGYQCKG KSHENQDGLEVSRIVVDPYAKILAPSIPKS QGLG P KFLGQIS VP FD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360
Query: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420
WDGEV PNLPMEKLFVYRLNV+RFT+DKSSQLPADIAGTFSGLTKKLLH KNLGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420
Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480
EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVI+EVVYTH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
Query: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
TS +GALQGIDDSSYYF NRVANLEE+SALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540
Query: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600
NASFLL+GHHGELLSRPPFVEAIAFDP+LSKTKLVADFWDPQEL SKETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660
Query: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
NSNLASELSWNCGEEGPT+ LKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720
Query: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780
VAFND+RSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQ+RNFLKGENIDWFDNNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780
Query: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840
PQWEDASCKFLAVMLRADKEENESITENPKTRS+IFM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840
Query: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAN 885
RVVDTALPFPGFFSSDGELVPMTGSVTYE+QAHSCALFEAKSAN
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884
BLAST of CsaV3_7G028580 vs. TrEMBL
Match:
tr|M5VNY8|M5VNY8_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_8G244800 PE=4 SV=1)
HSP 1 Score: 1129.0 bits (2919), Expect = 0.0e+00
Identity = 552/891 (61.95%), Postives = 693/891 (77.78%), Query Frame = 0
Query: 1 MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
MAT S+ IQ C +NCG+TES KL A++ + K + F K+D R L E
Sbjct: 1 MATLPLSIAIQA-CCFNCGITESSKLTATNGFRHRDKAMHGFVKLDAERKLVFRE----V 60
Query: 61 VKSSHRNLS-----KTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFV 120
V++ + NL K YAKS +SV QR G +++E +V+TYLFRTE GD+VNVFV
Sbjct: 61 VQNINENLPRDHNLKVYAKSQVSVEPMEQRFSTGTETEEIDKVSTYLFRTEIGDVVNVFV 120
Query: 121 GKKGSTFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTP-NFESSPPDETTGATETPF 180
K+ + +TVNIEVPS L S D L+L WG+YR+DS+ P +F+SS P++TT ETP
Sbjct: 121 RKRNAKYTVNIEVPSSHLSSNDRRLVLRWGMYRADSSCFVPLDFKSSTPNDTTTTLETPL 180
Query: 181 VKTSEGKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPS 240
++TS G+F++ELEF+AK PFY SF+L P + EIRSH+KT+F VPVGFGRGYP
Sbjct: 181 IQTSSGRFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPG 240
Query: 241 PLGLSISGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEG 300
PLGL+ S DGS+NF+IFS +AES+ LCLY+++T+EKP LELDLDPY+NRSG+IWH SF
Sbjct: 241 PLGLTFSNDGSMNFAIFSRNAESVALCLYDNTTAEKPALELDLDPYVNRSGDIWHVSFGS 300
Query: 301 ASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQIS 360
A FVSYGY+ KG+ N++ + +++DPYAK++A SIP + G GL K+LG++
Sbjct: 301 AWSFVSYGYKFKGNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGL----KYLGRLC 360
Query: 361 KVPTFDWDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLG 420
+ P FDW G+V P+L MEKL VYRLNV RFT KSSQLP +I G+FSGLT+KL HFK+LG
Sbjct: 361 EEPAFDWAGDVRPDLSMEKLVVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDLG 420
Query: 421 VNAVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIL 480
VNAVLLEPIF FDE++GPYFP HFFSP + +GPS +SA+NSMKEMV++ HANG+EV+L
Sbjct: 421 VNAVLLEPIFPFDEQKGPYFPHHFFSPMDCFGPSRGPVSAVNSMKEMVRKFHANGIEVLL 480
Query: 481 EVVYTHTSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHV 540
EVV+THT+ ALQGID SSYY N V +LE ++ALNCNYPIVQQL+LDSLRYWVTEFHV
Sbjct: 481 EVVFTHTAEGEALQGIDISSYYHVNEVEDLEARNALNCNYPIVQQLVLDSLRYWVTEFHV 540
Query: 541 DGFCFVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHW 600
DGF F+NAS LLRG +GE LSRPP VEAIAFDPLLSKTK++AD WDP + KET FPHW
Sbjct: 541 DGFFFINASSLLRGFNGEYLSRPPLVEAIAFDPLLSKTKIIADCWDPHGMAPKETHFPHW 600
Query: 601 KRWAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLV 660
+RWAEVN+KFC+D+R+F RGEGL+S LATRLCG+GD+FSDGRGPAF+FNFI+RN GLPLV
Sbjct: 601 RRWAEVNTKFCNDVRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFAFNFISRNSGLPLV 660
Query: 661 DLVSFSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECG 720
DLVSFS LASELSWNCGEEGPT+ VLE+RLKQIRNF+F+LFVSLGVPVLNMGDECG
Sbjct: 661 DLVSFSGVELASELSWNCGEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECG 720
Query: 721 QSSGGSVAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWF 780
QS+GGS A++D+++F+W+ L+T F TQTTQFIAFLSSFR RR DL Q RNFLK ENI W+
Sbjct: 721 QSTGGSPAYSDRKAFDWNALETGFATQTTQFIAFLSSFRKRRSDLLQKRNFLKEENIGWY 780
Query: 781 DNNQSPPQWEDASCKFLAVMLRADKEE-NESITENPKTRSNIFMVFNASDQSESVALPEP 840
+++Q+PP+WED S KFLA+ L+AD++E N+ E+ + ++F+ F+A+D SE+V LP P
Sbjct: 781 ESDQTPPRWEDPSRKFLAMRLKADEDEVNQPGDESSHSWGDLFVAFSAADHSETVVLPPP 840
Query: 841 LEGTSWFRVVDTALPFPGFFSSDGELV--PMTGSVTYEIQAHSCALFEAKS 883
LEG W R+VDTALPFPGFFS+DGE V + G YE+++HSCALFEA+S
Sbjct: 841 LEGMGWRRLVDTALPFPGFFSTDGEPVVEQIVGLFAYEMKSHSCALFEARS 882
BLAST of CsaV3_7G028580 vs. TrEMBL
Match:
tr|A0A2N9GWM1|A0A2N9GWM1_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS31596 PE=4 SV=1)
HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 558/891 (62.63%), Postives = 685/891 (76.88%), Query Frame = 0
Query: 1 MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
MAT SL IQP C CG + S KL+ + H +Y F KMD R L GE
Sbjct: 1 MATLPPSLAIQP-CNLKCGASGSSKLSPACHYICRNHIRYGFEKMDLERKLICGEAAQNV 60
Query: 61 VKSSHRNL-SKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKG 120
VKS ++L SK +A S + V + Q++ +++ +V+TYLF TE G V VFV K+
Sbjct: 61 VKSPCQHLNSKVFATSRVLVEEIEQKVSTATEAEGLEKVSTYLFWTEVGGQVKVFVRKRS 120
Query: 121 STFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTP-NFESSPPDETTGATETPFVKTS 180
+ VNIEV S+ L S ++ L LSWG YR+DS+ P + +SS PD +ETPF++TS
Sbjct: 121 VKYVVNIEVSSLPLHSSEDRLTLSWGRYRADSSSFMPLDVQSSEPDTRISTSETPFIQTS 180
Query: 181 EGKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSS--EIRSHKKTSFSVPVGFGRGYPSPL 240
G+F++ELEF+AK PFYLSF+LK + DSG S EIRSH+KT+F VPVG G GYP+PL
Sbjct: 181 AGRFALELEFEAKQFPFYLSFLLK--LQSDSGLSGLEIRSHRKTNFCVPVGLGTGYPAPL 240
Query: 241 GLSISGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGAS 300
GLS S DGS+NF+IFS AE ++LCLY+D+T+++P LELDLDPY+NRSG+IWHASFE A
Sbjct: 241 GLSFSPDGSMNFAIFSRYAEGVILCLYDDTTADEPALELDLDPYVNRSGDIWHASFESAW 300
Query: 301 KFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKV 360
FVSYGY+CKGS + + L+ +I++DPYAKI+ S P S G L K+LGQ+ K
Sbjct: 301 TFVSYGYRCKGSLLQRSNNKLDAGQIILDPYAKIIGNSSPSSHGS--KLLQKYLGQLCKE 360
Query: 361 PTFDWDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVN 420
P FDW + HP LPMEKL VYRLNV FT KSSQLP D+AGTFSGLT+KL HFK+LG+N
Sbjct: 361 PAFDWGDDTHPYLPMEKLLVYRLNVRHFTEHKSSQLPTDVAGTFSGLTEKLQHFKDLGMN 420
Query: 421 AVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEV 480
AVLLEPIF FDEK GPYFPFHFFSP N YGPSG S+SAINSMKEMVK++HANG+EV+LEV
Sbjct: 421 AVLLEPIFSFDEKNGPYFPFHFFSPMNLYGPSGGSVSAINSMKEMVKKMHANGIEVLLEV 480
Query: 481 VYTHTSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDG 540
V+THT+ G+LQGIDD SYY+ N+V +LE ALNCNYPIVQQL+LDSLR+WVTEFHVDG
Sbjct: 481 VFTHTAAAGSLQGIDDLSYYYANKVGDLEAPKALNCNYPIVQQLILDSLRHWVTEFHVDG 540
Query: 541 FCFVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKR 600
FCF+NAS LLRG HGE LSRPP +EAIAFDPLLSKTK++AD WDP ++ KE RFPHWKR
Sbjct: 541 FCFINASSLLRGFHGEYLSRPPLIEAIAFDPLLSKTKIIADCWDPHDMLPKENRFPHWKR 600
Query: 601 WAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDL 660
WAE+++KFC+D+R+F+RGEG++S LATRLCGSGD+FSDGRGPAFSFNFIARN GL LVDL
Sbjct: 601 WAEIDTKFCNDVRNFWRGEGVLSDLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDL 660
Query: 661 VSFSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQS 720
VSFS+ LAS+LSWNCGEEGPT N VLE+RLKQIRNF+FVL++SLGVP+LNMGDECGQS
Sbjct: 661 VSFSSDALASQLSWNCGEEGPTDNTTVLERRLKQIRNFLFVLYISLGVPILNMGDECGQS 720
Query: 721 SGGSVAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDN 780
SGGS A+ D++ F+W+ LKT FG QT+QFI+FLS FR RR DL Q RNFLK ENIDW
Sbjct: 721 SGGSPAYTDRKPFDWNALKTGFGIQTSQFISFLSLFRMRRSDLLQKRNFLKEENIDWHGR 780
Query: 781 NQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEG 840
+Q PP+WED S +FLA+ LRADK E+ TE+ R ++F+ FNA+D SESV LP P EG
Sbjct: 781 DQFPPKWEDPSRRFLAMTLRADKVESPLSTESSHLRGDLFIAFNAADHSESVILPTPPEG 840
Query: 841 TSWFRVVDTALPFPGFFSSDGELVPMTGS--VTYEIQAHSCALFEAKSAND 886
SW +VDTALPFPGFFS++GE +P + V Y++++HSCALFEA S ++
Sbjct: 841 MSWLCMVDTALPFPGFFSTNGEPLPKQKAELVAYKMKSHSCALFEASSPSN 886
BLAST of CsaV3_7G028580 vs. TrEMBL
Match:
tr|A0A2P4IIC1|A0A2P4IIC1_QUESU (Isoamylase 2, chloroplastic OS=Quercus suber OX=58331 GN=CFP56_26168 PE=4 SV=1)
HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 552/889 (62.09%), Postives = 676/889 (76.04%), Query Frame = 0
Query: 1 MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60
MAT SL IQ + CG+++ K + + H Y +Y F KMD R L E
Sbjct: 1 MATLPPSLAIQSGYL-KCGISDVSKSSPACHYIYRNHIRYGFEKMDLERRLICDEVSQNV 60
Query: 61 VKSSHRNL-SKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKG 120
VKSS RNL S+ +A S + V + Q++ ++ + + TYLF TE G V VFV K
Sbjct: 61 VKSSCRNLNSRVFATSRVLVEEIEQKVSTITEADNREKALTYLFWTEVGGQVKVFVRKTS 120
Query: 121 STFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTP-NFESSPPDETTGATETPFVKTS 180
+ VNIEV S+QL S ++ L+ SWG YR+DS+ P + +SS PD T +ETPF++TS
Sbjct: 121 VKYAVNIEVSSLQLQSSEDRLISSWGRYRADSSSFIPLDVQSSVPDSRTPTSETPFIQTS 180
Query: 181 EGKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGL 240
G+F++ELEF+AK PFYLSF+LK P S SEIRSH+KT F VPVGF +GYP+PLGL
Sbjct: 181 AGRFALELEFEAKQIPFYLSFILKLPSDSGSRGSEIRSHRKTKFCVPVGFRKGYPAPLGL 240
Query: 241 SISGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKF 300
S S DGS+NF+IFS + E ++LC Y+D+ +E+P LELDLDPYINRSG+IWHASFE A F
Sbjct: 241 SFSPDGSMNFAIFSRNVEGVILCFYDDAKAEEPALELDLDPYINRSGDIWHASFESAWTF 300
Query: 301 VSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPT 360
VSYGYQCKGS N + + +I++DPYAKI+ SIP S G K+LG++ K P
Sbjct: 301 VSYGYQCKGSLLQRNNNKFDAGQIILDPYAKIIVNSIPSSDGS-----QKYLGRLCKEPG 360
Query: 361 FDWDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAV 420
FDW + HP LPMEKL VYRLNV FT KSSQL D+AGTF GLT+KL HFK+LG+NAV
Sbjct: 361 FDWGDDTHPYLPMEKLVVYRLNVRHFTEHKSSQLATDVAGTFIGLTEKLQHFKDLGMNAV 420
Query: 421 LLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVY 480
LLEPIF FDEK GPYFP HFFSPTN YGPSG S+SAINSMKEMVK+LHANG+EV+LEVV+
Sbjct: 421 LLEPIFSFDEKNGPYFPCHFFSPTNLYGPSGGSVSAINSMKEMVKKLHANGIEVLLEVVF 480
Query: 481 THTSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFC 540
THT+ G+LQGIDD SYY N +LE + LNCNYPIVQQL+LDSL+YWVTEFH+DGFC
Sbjct: 481 THTAVAGSLQGIDDISYYHVNEGEDLEATNYLNCNYPIVQQLILDSLQYWVTEFHIDGFC 540
Query: 541 FVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWA 600
F+NAS LLRG HGE LSRPP VEAI+FDPLLSKTK++AD WDP ++ KETRFPHWKRWA
Sbjct: 541 FINASSLLRGFHGEYLSRPPLVEAISFDPLLSKTKIIADCWDPHDMLPKETRFPHWKRWA 600
Query: 601 EVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVS 660
E+++KFC+D+R+F+RGEGL+S LATRLCGSGD+FSDGRGPAFSFNFIARN GL LVDLVS
Sbjct: 601 EIDTKFCNDVRNFWRGEGLLSDLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVDLVS 660
Query: 661 FSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSG 720
FSN LAS+LSWNCGEEGPT N VLE+RLKQIRNF+FVL++SLGVP+LNMGDECGQS G
Sbjct: 661 FSNDALASQLSWNCGEEGPTDNTTVLERRLKQIRNFLFVLYISLGVPILNMGDECGQSCG 720
Query: 721 GSVAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQ 780
GS A++D++ +W+ LKT F QT+QFI+FLSS R RR DL Q RNFLK ENI+W ++Q
Sbjct: 721 GSPAYSDRKPLDWNTLKTGFSIQTSQFISFLSSLRMRRSDLLQKRNFLKEENIEWHGSDQ 780
Query: 781 SPPQWEDASCKFLAVMLRADKEENESITENPK--TRSNIFMVFNASDQSESVALPEPLEG 840
SPP+WED SC+FLA+ LRAD+ E+ +E+ R ++F+ FNA+D SESV LP+P EG
Sbjct: 781 SPPKWEDPSCRFLAMTLRADEVESSLNSESSSHVRRGDLFIAFNAADLSESVILPQPPEG 840
Query: 841 TSWFRVVDTALPFPGFFSSDGELVP--MTGSVTYEIQAHSCALFEAKSA 884
SW +VDTALPFPGFFS+ GE +P M SV YE+++HSCALFEA S+
Sbjct: 841 MSWRCLVDTALPFPGFFSTSGETLPEQMAESVAYEMKSHSCALFEASSS 883
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_004136188.1 | 0.0e+00 | 100.00 | PREDICTED: isoamylase 2, chloroplastic [Cucumis sativus] >KGN44865.1 hypothetica... | [more] |
| XP_008451396.1 | 0.0e+00 | 93.89 | PREDICTED: isoamylase 2, chloroplastic [Cucumis melo] | [more] |
| XP_022953509.1 | 0.0e+00 | 83.16 | isoamylase 2, chloroplastic isoform X1 [Cucurbita moschata] >XP_022953510.1 isoa... | [more] |
| XP_023548355.1 | 0.0e+00 | 82.94 | isoamylase 2, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023548356.1 isoamyl... | [more] |
| XP_022953511.1 | 0.0e+00 | 82.49 | isoamylase 2, chloroplastic isoform X2 [Cucurbita moschata] | [more] |
