Carg07231 (gene) Silver-seed gourd

BLAST of Carg07231 vs. NCBI nr
Match: XP_022953509.1 (isoamylase 2, chloroplastic isoform X1 [Cucurbita moschata] >XP_022953510.1 isoamylase 2, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 1766.9 bits (4575), Expect = 0.0e+00
Identity = 864/884 (97.74%), Postives = 874/884 (98.87%), Query Frame = 0

Query: 24  MATFLSSLIQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGFT 83
           MATFLSSLIQPFCGYNRGGTESFKLAV+DYHTYGQKMNYQSGKLDEARMQAHGESKVGFT
Sbjct: 1   MATFLSSLIQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGFT 60

Query: 84  KSLHRNVCKAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGSG 143
           KSLHRNVC+AYAPS ISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGSG
Sbjct: 61  KSLHRNVCRAYAPSRISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGSG 120

Query: 144 FLVSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEGK 203
           F+ SIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEGK
Sbjct: 121 FMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEGK 180

Query: 204 FAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSIS 263
           FAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSIS
Sbjct: 181 FAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSIS 240

Query: 264 GDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGASNFVSY 323
           GDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWH SFEGASNFVSY
Sbjct: 241 GDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVSY 300

Query: 324 GYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSKFLGQICNVPAFSW 383
           GYRCRGSKFHENQDEAEVSRILVDPYAKILTAS PKTRGQG+GLPSKFLGQICNVPAFSW
Sbjct: 301 GYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFSW 360

Query: 384 DGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLLE 443
           DGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPID AGTFSGLTQKLLHFKKLGVNAVLLE
Sbjct: 361 DGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLLE 420

Query: 444 PIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTHT 503
           PIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTHT
Sbjct: 421 PIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTHT 480

Query: 504 AMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFIN 563
           AMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLR+WVTEFHIDGFCFIN
Sbjct: 481 AMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFIN 540

Query: 564 ASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAELN 623
           ASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIA+FWDPQE VPKETRFPHWKRWAELN
Sbjct: 541 ASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAELN 600

Query: 624 SKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSS 683
           SKFSND+KEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSS
Sbjct: 601 SKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSS 660

Query: 684 SKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSV 743
           SKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSV
Sbjct: 661 SKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSV 720

Query: 744 AFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGENIDWFDNNQSPP 803
           AFKDRKSFNWDSLKTDFGTQTT+FI F SSFRSRRFDLFQSR+FLK ENIDWFDNNQSPP
Sbjct: 721 AFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSPP 780

Query: 804 EWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWFR 863
           +WEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWFR
Sbjct: 781 DWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWFR 840

Query: 864 LVDTALSFPGFFSSDGEPVPTTSPVTYEMQAHSCTLFEAKSFND 908
           LVDTALSFPGFFSSDGEPVPTTSPVTY MQAHSCTLFEAKSFND
Sbjct: 841 LVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFND 884

BLAST of Carg07231 vs. NCBI nr
Match: XP_023548355.1 (isoamylase 2, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023548356.1 isoamylase 2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1764.6 bits (4569), Expect = 0.0e+00
Identity = 861/884 (97.40%), Postives = 871/884 (98.53%), Query Frame = 0

Query: 24  MATFLSSLIQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGFT 83
           MATFLSSLIQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGFT
Sbjct: 1   MATFLSSLIQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGFT 60

Query: 84  KSLHRNVCKAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGSG 143
           KSLHRNVC+AYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGSG
Sbjct: 61  KSLHRNVCRAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGSG 120

Query: 144 FLVSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEGK 203
           F+V+IEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATE PFVRTSEGK
Sbjct: 121 FMVNIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATEAPFVRTSEGK 180

Query: 204 FAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSIS 263
           FAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSIS
Sbjct: 181 FAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSIS 240

Query: 264 GDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGASNFVSY 323
           GDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWH SFEGASNFVSY
Sbjct: 241 GDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVSY 300

Query: 324 GYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSKFLGQICNVPAFSW 383
           GYRCRGSKFHENQDEAEVSRILVDPYAKILTAS PKTRGQG+GLPSKFLGQICNVPAFSW
Sbjct: 301 GYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFSW 360

Query: 384 DGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLLE 443
           DGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLLE
Sbjct: 361 DGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLLE 420

Query: 444 PIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTHT 503
           PIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVK LHANGVEVILEVVFTHT
Sbjct: 421 PIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKVLHANGVEVILEVVFTHT 480

Query: 504 AMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFIN 563
           AMDGALQGIDDSCYYFVNRV NSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFIN
Sbjct: 481 AMDGALQGIDDSCYYFVNRVTNSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFIN 540

Query: 564 ASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAELN 623
           ASSLLQGHHGELLSRPPF EAIAFDPLLSKTKIIA+FWDPQE VPKE RFPHWKRWAELN
Sbjct: 541 ASSLLQGHHGELLSRPPFAEAIAFDPLLSKTKIIADFWDPQESVPKEIRFPHWKRWAELN 600

Query: 624 SKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSS 683
           SKFSND+KEFFRGEGLV SLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSS
Sbjct: 601 SKFSNDIKEFFRGEGLVRSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSS 660

Query: 684 SKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSV 743
           SKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSV
Sbjct: 661 SKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSV 720

Query: 744 AFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGENIDWFDNNQSPP 803
           AFKDRKSFNWDSLKTDFGTQTT+FI F SSFRSRRFDLFQSR+FLK ENIDWFDNNQSPP
Sbjct: 721 AFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSPP 780

Query: 804 EWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWFR 863
           +WEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGT WFR
Sbjct: 781 DWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTTWFR 840

Query: 864 LVDTALSFPGFFSSDGEPVPTTSPVTYEMQAHSCTLFEAKSFND 908
           LVDTALSFPGFFSSDGEPVPTTSP+TYEMQAHSCTLFEAKSFND
Sbjct: 841 LVDTALSFPGFFSSDGEPVPTTSPITYEMQAHSCTLFEAKSFND 884

BLAST of Carg07231 vs. NCBI nr
Match: XP_022953511.1 (isoamylase 2, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 1738.4 bits (4501), Expect = 0.0e+00
Identity = 854/884 (96.61%), Postives = 864/884 (97.74%), Query Frame = 0

Query: 24  MATFLSSLIQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGFT 83
           MATFLSSLIQPFCGYNRGGTESFKLAV+DYHTYGQKMNYQSGKLDEARMQAHGESKVGFT
Sbjct: 1   MATFLSSLIQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGFT 60

Query: 84  KSLHRNVCKAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGSG 143
           KSLHRNVC+AYAPS ISVGKS          EEDRKVATYLFRTEFGDLVYAFVGKRGSG
Sbjct: 61  KSLHRNVCRAYAPSRISVGKS----------EEDRKVATYLFRTEFGDLVYAFVGKRGSG 120

Query: 144 FLVSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEGK 203
           F+ SIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEGK
Sbjct: 121 FMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEGK 180

Query: 204 FAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSIS 263
           FAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSIS
Sbjct: 181 FAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSIS 240

Query: 264 GDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGASNFVSY 323
           GDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWH SFEGASNFVSY
Sbjct: 241 GDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVSY 300

Query: 324 GYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSKFLGQICNVPAFSW 383
           GYRCRGSKFHENQDEAEVSRILVDPYAKILTAS PKTRGQG+GLPSKFLGQICNVPAFSW
Sbjct: 301 GYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFSW 360

Query: 384 DGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLLE 443
           DGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPID AGTFSGLTQKLLHFKKLGVNAVLLE
Sbjct: 361 DGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLLE 420

Query: 444 PIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTHT 503
           PIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTHT
Sbjct: 421 PIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTHT 480

Query: 504 AMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFIN 563
           AMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLR+WVTEFHIDGFCFIN
Sbjct: 481 AMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFIN 540

Query: 564 ASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAELN 623
           ASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIA+FWDPQE VPKETRFPHWKRWAELN
Sbjct: 541 ASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAELN 600

Query: 624 SKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSS 683
           SKFSND+KEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSS
Sbjct: 601 SKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSS 660

Query: 684 SKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSV 743
           SKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSV
Sbjct: 661 SKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSV 720

Query: 744 AFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGENIDWFDNNQSPP 803
           AFKDRKSFNWDSLKTDFGTQTT+FI F SSFRSRRFDLFQSR+FLK ENIDWFDNNQSPP
Sbjct: 721 AFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSPP 780

Query: 804 EWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWFR 863
           +WEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWFR
Sbjct: 781 DWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWFR 840

Query: 864 LVDTALSFPGFFSSDGEPVPTTSPVTYEMQAHSCTLFEAKSFND 908
           LVDTALSFPGFFSSDGEPVPTTSPVTY MQAHSCTLFEAKSFND
Sbjct: 841 LVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFND 874

BLAST of Carg07231 vs. NCBI nr
Match: XP_022992495.1 (isoamylase 2, chloroplastic [Cucurbita maxima] >XP_022992496.1 isoamylase 2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1726.1 bits (4469), Expect = 0.0e+00
Identity = 847/884 (95.81%), Postives = 858/884 (97.06%), Query Frame = 0

Query: 24  MATFLSSLIQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGFT 83
           MATFLSSLIQPFCGYNRGGTESFKLAVNDYHTYGQKMNY SGKLDEARMQAHGESKVGF 
Sbjct: 1   MATFLSSLIQPFCGYNRGGTESFKLAVNDYHTYGQKMNYHSGKLDEARMQAHGESKVGFI 60

Query: 84  KSLHRNVCKAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGSG 143
           KSLHRNVCKAYAPSGISVGKS          EEDR+VATYLFRTEFGDLV  FVGKRGSG
Sbjct: 61  KSLHRNVCKAYAPSGISVGKS----------EEDRQVATYLFRTEFGDLVNVFVGKRGSG 120

Query: 144 FLVSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEGK 203
           F+V+IEISS KLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEGK
Sbjct: 121 FMVNIEISSSKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEGK 180

Query: 204 FAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSIS 263
           FAVELEFDAKHDPFYLSFLLKYPVGV SGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSIS
Sbjct: 181 FAVELEFDAKHDPFYLSFLLKYPVGVHSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSIS 240

Query: 264 GDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGASNFVSY 323
           GDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWH SF GASNFVSY
Sbjct: 241 GDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFGGASNFVSY 300

Query: 324 GYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSKFLGQICNVPAFSW 383
           GYRCRGSKFHENQDEAEV RILVDPYAKILTAS PKTRG+G+GLPSKFLGQICNVPAFSW
Sbjct: 301 GYRCRGSKFHENQDEAEVCRILVDPYAKILTASIPKTRGRGLGLPSKFLGQICNVPAFSW 360

Query: 384 DGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLLE 443
           DGEVRPNLPMEKLF+YRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLLE
Sbjct: 361 DGEVRPNLPMEKLFVYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLLE 420

Query: 444 PIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTHT 503
           PIFPFDEKDGPYFPFHFFSPTNIYVPS ASISSLNSVKEMVKELHANGVEVILEVVFTHT
Sbjct: 421 PIFPFDEKDGPYFPFHFFSPTNIYVPSEASISSLNSVKEMVKELHANGVEVILEVVFTHT 480

Query: 504 AMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFIN 563
           AMDGALQ IDDSCYYFVNRVANSEGKSALNCNHPIV+QLLLDSLRYWVTEFHIDGFCFIN
Sbjct: 481 AMDGALQRIDDSCYYFVNRVANSEGKSALNCNHPIVEQLLLDSLRYWVTEFHIDGFCFIN 540

Query: 564 ASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAELN 623
           ASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIA+FWDPQE +PKETRFPHWKRWAELN
Sbjct: 541 ASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESLPKETRFPHWKRWAELN 600

Query: 624 SKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSS 683
           SKFSND+KE FRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSS
Sbjct: 601 SKFSNDIKELFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSS 660

Query: 684 SKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSV 743
           SKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSV
Sbjct: 661 SKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSV 720

Query: 744 AFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGENIDWFDNNQSPP 803
           AFKDRKSFNWDSLKTDFGTQTT+FI F SSFRSRRFDLFQSRHFLKGENIDWFDNNQSPP
Sbjct: 721 AFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRHFLKGENIDWFDNNQSPP 780

Query: 804 EWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWFR 863
           EWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWFR
Sbjct: 781 EWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWFR 840

Query: 864 LVDTALSFPGFFSSDGEPVPTTSPVTYEMQAHSCTLFEAKSFND 908
           LVDTALSFPGFFSSDGEPVPTTSPVTYEMQAHSCTLFEAKSFND
Sbjct: 841 LVDTALSFPGFFSSDGEPVPTTSPVTYEMQAHSCTLFEAKSFND 874

BLAST of Carg07231 vs. NCBI nr
Match: XP_008451396.1 (PREDICTED: isoamylase 2, chloroplastic [Cucumis melo])

HSP 1 Score: 1526.9 bits (3952), Expect = 0.0e+00
Identity = 744/884 (84.16%), Postives = 806/884 (91.18%), Query Frame = 0

Query: 24  MATFLSSL-IQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 83
           MATFLSSL IQPFC +N G TES KLAV+D+ TYGQK  YQSGK+DEARM AHGESKVG 
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 84  TKSLHRNVCKAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 143
            KS HRN+ KAY  SGI VGKSGQ+ G GGK++E RKVATYLFRTEFGDLV  FVGK GS
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 144 GFLVSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 203
            F V+IEISS++L S DETLLLSW VYRS+SA VT NFQS  P+ T GATETPFV+TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 204 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 263
           KF+VELEFDAKH PFYLSF+LK+P+GVDSGSSEI+SHRKTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 264 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGASNFVS 323
           SGDGSINFSIFSSTAESVVLCLYNDSTSEKP+LELDLDPYINRSGNIWH SFEGASNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 324 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSKFLGQICNVPAFS 383
           YGY+C+G K HENQD  EVSRI+VDPYAKIL  S PK+R QG+G P KFLGQI  VPAF 
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 384 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLL 443
           WDGEVRPNLPMEKLF+YRLNVK FT+DKSSQLP DIAGTFSGLT+KLLH K LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 444 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 503
           EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS++NS+KEMVKELHANGVEVI+EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 504 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFI 563
           T++DGALQGIDDS YYF NRVAN E +SALNCN+PIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 564 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAEL 623
           NAS LLQGHHGELLSRPPFVEAIAFDP+LSKTK++A+FWDPQELV KETRFPHWKRWAE+
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 624 NSKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 683
           NSKF +D+++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 684 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 743
           +S LASELSWNCGEEGPT+ ++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 744 VAFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGENIDWFDNNQSP 803
           VAF DR+SFNWD LKTDFGTQTT+FIAF SSFRSRRFDLFQSR+FLKGENIDWFDNNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 804 PEWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 863
           P+WED SCKFLAVMLRADKEE+ SITENPK +SDIFMAFNASDQS SVALPEPSEGT+WF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 864 RLVDTALSFPGFFSSDGEPVPTTSPVTYEMQAHSCTLFEAKSFN 907
           R+VDTAL FPGFFSSDGE VP T  VTYEMQAHSC LFEAKS N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

BLAST of Carg07231 vs. TAIR10
Match: AT1G03310.2 (debranching enzyme 1)

HSP 1 Score: 915.6 bits (2365), Expect = 2.4e-266
Identity = 444/799 (55.57%), Postives = 589/799 (73.72%), Query Frame = 0

Query: 113 KTEEDRKVATYLFRTEFGDLVYAFVGKRGSGFLVSIEISSLKLASIDET-LLLSWGVYRS 172
           + ++ +KV +Y FRT+ G LV   V K+   + + + +SSL+L+  D++ L++ WGVYRS
Sbjct: 88  EADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSLELSGDDKSRLVMVWGVYRS 147

Query: 173 NSAIVTP-NFQSILPEETIGATETPFVRTSEGKFAVELEFDAKHDPFYLSFLLKYPVGVD 232
           +S+   P +F++   +     TET FV++S  +  + LEFD K  PFYLSF LK   G D
Sbjct: 148 DSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFDGKESPFYLSFHLKLVSGRD 207

Query: 233 SGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSIS-GDGSINFSIFSSTAESVVLCLYNDST 292
               E+ +HR T F +PVGF  G+P PLGLS    D S NFS FS ++ +VVLCLY+DST
Sbjct: 208 PDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDST 267

Query: 293 SEKPVLELDLDPYINRSGNIWHVSFEGASNFVSYGYRCRGSKFHENQDEAEVSRILVDPY 352
           ++KP LELDLDPY+NR+G++WH S +   +FV YGYRC+ +   +   + E   I++DPY
Sbjct: 268 TDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPY 327

Query: 353 AKILTASTPKTRGQGVGLPSKFLGQICNVPAFSWDGEVRPNLPMEKLFIYRLNVKHFTVD 412
           A ++          G  +  K+LG +   P+F W  +V PN+P+EKL +YRLNVK FT  
Sbjct: 328 ATVV----------GKSVSQKYLGSLSKSPSFDWGEDVSPNIPLEKLLVYRLNVKGFTQH 387

Query: 413 KSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLLEPIFPFDEKDGPYFPFHFFSPTNIYVP 472
           +SS+LP ++AGTFSG+ +K+ H K LG NAVLLEPIF F E+ GPYFPFHFFSP +IY P
Sbjct: 388 RSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFHFFSPMDIYGP 447

Query: 473 SGASISSLNSVKEMVKELHANGVEVILEVVFTHTAMDGALQGIDDSCYYFVNRVANSEGK 532
           S +  S++NS+K MVK+LH+ G+EV+LEVVFTHTA  GAL+GIDDS YY+  R  + + K
Sbjct: 448 SNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRGIDDSSYYYKGRANDLDSK 507

Query: 533 SALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLQGHHGELLSRPPFVEAIAFDP 592
           S LNCN+P+VQQL+L+SLRYWVTEFH+DGFCFINASSLL+G HGE LSRPP VEAIAFDP
Sbjct: 508 SYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHGEQLSRPPLVEAIAFDP 567

Query: 593 LLSKTKIIAEFWDPQELVPKETRFPHWKRWAELNSKFSNDMKEFFRGEGLVSSLATRLCG 652
           LL++TK+IA+ WDP E++PKE RFPHWKRWAELN+++  +++ F RG G++S LATR+CG
Sbjct: 568 LLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGRGVLSDLATRICG 627

Query: 653 SGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSSSKLASELSWNCGEEGPTSNIEVLEKR 712
           SGDVF+DGRGPAFSFN+I+RN GL LVD+VSFS  +LASELSWNCGEEG T+   VL++R
Sbjct: 628 SGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELSWNCGEEGATNKSAVLQRR 687

Query: 713 LKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFKDRKSFNWDSLKTDFGTQTTRFIA 772
           LKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS   + RK F+W+ L + FGTQ T+FI+
Sbjct: 688 LKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPFDWNLLASAFGTQITQFIS 747

Query: 773 FFSSFRSRRFDLFQSRHFLKGENIDWFDNNQSPPEWEDPSCKFLAVMLR--ADKEESGSI 832
           F +S R+RR D+FQ R FLK ENI W+ N+Q+ P+WEDP+ KFLA+ ++  +++EE+ S+
Sbjct: 748 FMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEIKSESEEEETASL 807

Query: 833 TENPKPQS-DIFMAFNASDQSVSVALPEPSEGTNWFRLVDTALSFPGFFSSDGEPVPTTS 892
            E  +P+S D+F+ FNASD   SV LP   +G+ W RLVDTAL FPGFFS +GE V    
Sbjct: 808 AEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALPFPGFFSVEGETVVAEE 867

Query: 893 P----VTYEMQAHSCTLFE 902
           P    V YEM+ +SCTLFE
Sbjct: 868 PLQQLVVYEMKPYSCTLFE 876

BLAST of Carg07231 vs. TAIR10
Match: AT4G09020.1 (isoamylase 3)

HSP 1 Score: 379.0 bits (972), Expect = 8.1e-105
Identity = 253/709 (35.68%), Postives = 361/709 (50.92%), Query Frame = 0

Query: 253 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----NDSTSEKPVLELDLDPYINRSG 312
           G  SPLG+S   D  INF++FS  A SV LCL       D T +  ++EL LDP +N++G
Sbjct: 88  GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 313 NIWHVSFEGAS-NFVSYGYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVG 372
           + WH+  E    N V YGYR  G    +     + S +L+DPYAK++   +  + G    
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGHS--SFGDSSQ 207

Query: 373 LPSKFLGQI-CNVPAFSWDGEVR-PNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSG 432
             ++F G        F W  + + PN+P + L IY +NV+ FT D+SS +   I G++ G
Sbjct: 208 KFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267

Query: 433 LTQKLLHFKKLGVNAVLLEPIFPFDE-----KDGP---------YFPFHFFSPTNIYVP- 492
             +K+ H + LG+NAV L P+F FDE     +  P         Y   +FF+P + Y   
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327

Query: 493 SGASISSLNSVKEMVKELHANGVEVILEVVFTHT-------AMDGALQGIDDSCYYFV-- 552
            G  I +    KEMVK LH+ G+EVIL+VV+ HT           + +GID+  YY +  
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387

Query: 553 -NRVANSEG-KSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLQGHHGELLSR 612
            N++ N  G  + LNCNHP+V +L+LDSLR+WVTE+H+DGF F  AS L +   G  LS 
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447

Query: 613 PPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAELNSKFSNDMKEFFRGE- 672
           PP + AIA D +LS+ KIIAE WD   L     +FP+W RWAE N  + +D++ F +G+ 
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWDCGGLY-LVGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507

Query: 673 GLVSSLATRLCGSGDVFS-DGRGPAFSFNFIARNVGLPLVDLVSFS-----------SSK 732
           G+  S ATR+ GS D++  + R P    NF+  + G  L DLVS++           +  
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567

Query: 733 LASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAF 792
                SWNCG EG T +  +   R +Q++NF   L +S G P++ MGDE G +  G    
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYG---- 627

Query: 793 KDRKSFNWDSLKTDF-----GTQTTRFIAFFSSFRSRRFDLFQSRHFLKGEN------ID 852
            +  S+  D+   +F       +      FFS     R     S H LK EN      I 
Sbjct: 628 -NNNSYGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFR----HSHHVLKHENFLTQGEIT 687

Query: 853 WFDNNQSPPEWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPE 904
           W ++N     W++   KFLA  L    +  G          DI++AFNA D  V   +P+
Sbjct: 688 WHEDN-----WDNSESKFLAFTLH---DGIGG--------RDIYVAFNAHDYFVKALIPQ 747

BLAST of Carg07231 vs. TAIR10
Match: AT2G39930.1 (isoamylase 1)

HSP 1 Score: 338.6 bits (867), Expect = 1.2e-92
Identity = 233/690 (33.77%), Postives = 343/690 (49.71%), Query Frame = 0

Query: 253 GYPSPLGLSISGDGSINFSIFSSTAESVVLCL--YNDSTSEKPVLELDLDPYINRSGNIW 312
           G PSP G ++  DG +NFS++S+ + S  +CL   +D    K   E+ LDP  NR+G++W
Sbjct: 72  GLPSPFGPTVRDDG-VNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVW 131

Query: 313 HVSFEGASNFVSYGYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSK 372
           HV   G    + YGYR  G    E     + S IL+DPYAK + +   +     +G    
Sbjct: 132 HVFLRGDFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAIIS---RDEFGVLGPDDN 191

Query: 373 FLGQI-CNVPA----FSWDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGL 432
              Q+ C VP     F W+G++   LP + L IY ++V+ FT  +SS+  I+  GT+ G+
Sbjct: 192 CWPQMACMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSK--IEFPGTYQGV 251

Query: 433 TQKLLHFKKLGVNAVLLEPIFPFDEKD---------------GPYFPFHFFSPTNIYVPS 492
            +KL H K+LG+N + L P   F+E +                 Y    FFSP   Y  +
Sbjct: 252 AEKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASA 311

Query: 493 GASI---SSLNSVKEMVKELHANGVEVILEVVFTHTAMDG------ALQGIDDSCYYFV- 552
            ++     ++N  K +VKE H  G+EVI++VV  HTA         + +G+D+S YY + 
Sbjct: 312 SSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLA 371

Query: 553 --NRVANSEG-KSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCF------INASSLLQGH 612
                 N  G  +  NCNHP+V+Q +LD LRYWVTE H+DGF F        +SSL    
Sbjct: 372 PKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAA 431

Query: 613 H-------GELL------SRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKR 672
           +       G+LL      S PP ++ I+ DP+L   K+IAE WD   L  +   FPHW  
Sbjct: 432 NVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLY-QVGMFPHWGI 491

Query: 673 WAELNSKFSNDMKEFFRG-EGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVD 732
           W+E N KF + +++F +G +G   + A  LCGS +++  GR P  S NFI  + G  L D
Sbjct: 492 WSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLAD 551

Query: 733 LVSFSS-SKLAS----------ELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGV 792
           LV++++ + LA+            SWNCGEEG  ++I V   R +Q+RNF   L VS GV
Sbjct: 552 LVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGV 611

Query: 793 PVLNMGDECGQSSGGS----VAFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLF 852
           P++ MGDE G + GG+            F WD  K +  +   RF      FR    +  
Sbjct: 612 PMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDK-KEEAHSDFFRFCRILIKFRD-ECESL 671

Query: 853 QSRHFLKGENIDWFDNNQSPPEWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFN 873
               F   + + W       P W + S +F+A           S+ ++ K   +I++AFN
Sbjct: 672 GLNDFPTAKRLQWHGLAPEIPNWSETS-RFVAF----------SLVDSVK--KEIYVAFN 731

BLAST of Carg07231 vs. TAIR10
Match: AT3G20440.2 (Alpha amylase family protein)

HSP 1 Score: 63.2 bits (152), Expect = 9.8e-10
Identity = 56/202 (27.72%), Postives = 93/202 (46.04%), Query Frame = 0

Query: 380 AFSWDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLL-HFKKLGVN 439
           A+ W    +P +P E L IY     H  +  S         TF   T+K+L H K+ G N
Sbjct: 384 AYKWKYS-KPKVP-ESLRIYEC---HVGISGSEPK----VSTFEEFTKKVLPHVKRAGYN 443

Query: 440 AVLLEPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEV 499
           A+ L  I   + KD  YF    +  TN +  S +   + +  K +V E H  G+ V L++
Sbjct: 444 AIQL--IGVPEHKD--YFTVG-YRVTNFFAAS-SRYGTPDDFKRLVDEAHGLGLLVFLDI 503

Query: 500 VFTHTAMD-----GALQGIDDSCYYFVNRVANSE--GKSALNCNHPIVQQLLLDSLRYWV 559
           V ++ A D         G +D CY+   +  + +  G          V   L+ +L +W+
Sbjct: 504 VHSYAAADQMVGLSLFDGSND-CYFHYGKRGHHKHWGTRMFKYGDLDVLHFLISNLNWWI 563

Query: 560 TEFHIDGFCFINASSLLQGHHG 574
           TE+ +DG+ F + +S++  H+G
Sbjct: 564 TEYQVDGYQFHSLASMIYTHNG 569

BLAST of Carg07231 vs. Swiss-Prot
Match: sp|Q8L735|ISOA2_ARATH (Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2)

HSP 1 Score: 915.6 bits (2365), Expect = 4.3e-265
Identity = 444/799 (55.57%), Postives = 589/799 (73.72%), Query Frame = 0

Query: 113 KTEEDRKVATYLFRTEFGDLVYAFVGKRGSGFLVSIEISSLKLASIDET-LLLSWGVYRS 172
           + ++ +KV +Y FRT+ G LV   V K+   + + + +SSL+L+  D++ L++ WGVYRS
Sbjct: 88  EADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSLELSGDDKSRLVMVWGVYRS 147

Query: 173 NSAIVTP-NFQSILPEETIGATETPFVRTSEGKFAVELEFDAKHDPFYLSFLLKYPVGVD 232
           +S+   P +F++   +     TET FV++S  +  + LEFD K  PFYLSF LK   G D
Sbjct: 148 DSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFDGKESPFYLSFHLKLVSGRD 207

Query: 233 SGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSIS-GDGSINFSIFSSTAESVVLCLYNDST 292
               E+ +HR T F +PVGF  G+P PLGLS    D S NFS FS ++ +VVLCLY+DST
Sbjct: 208 PDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDST 267

Query: 293 SEKPVLELDLDPYINRSGNIWHVSFEGASNFVSYGYRCRGSKFHENQDEAEVSRILVDPY 352
           ++KP LELDLDPY+NR+G++WH S +   +FV YGYRC+ +   +   + E   I++DPY
Sbjct: 268 TDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPY 327

Query: 353 AKILTASTPKTRGQGVGLPSKFLGQICNVPAFSWDGEVRPNLPMEKLFIYRLNVKHFTVD 412
           A ++          G  +  K+LG +   P+F W  +V PN+P+EKL +YRLNVK FT  
Sbjct: 328 ATVV----------GKSVSQKYLGSLSKSPSFDWGEDVSPNIPLEKLLVYRLNVKGFTQH 387

Query: 413 KSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLLEPIFPFDEKDGPYFPFHFFSPTNIYVP 472
           +SS+LP ++AGTFSG+ +K+ H K LG NAVLLEPIF F E+ GPYFPFHFFSP +IY P
Sbjct: 388 RSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFHFFSPMDIYGP 447

Query: 473 SGASISSLNSVKEMVKELHANGVEVILEVVFTHTAMDGALQGIDDSCYYFVNRVANSEGK 532
           S +  S++NS+K MVK+LH+ G+EV+LEVVFTHTA  GAL+GIDDS YY+  R  + + K
Sbjct: 448 SNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRGIDDSSYYYKGRANDLDSK 507

Query: 533 SALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLQGHHGELLSRPPFVEAIAFDP 592
           S LNCN+P+VQQL+L+SLRYWVTEFH+DGFCFINASSLL+G HGE LSRPP VEAIAFDP
Sbjct: 508 SYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHGEQLSRPPLVEAIAFDP 567

Query: 593 LLSKTKIIAEFWDPQELVPKETRFPHWKRWAELNSKFSNDMKEFFRGEGLVSSLATRLCG 652
           LL++TK+IA+ WDP E++PKE RFPHWKRWAELN+++  +++ F RG G++S LATR+CG
Sbjct: 568 LLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGRGVLSDLATRICG 627

Query: 653 SGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSSSKLASELSWNCGEEGPTSNIEVLEKR 712
           SGDVF+DGRGPAFSFN+I+RN GL LVD+VSFS  +LASELSWNCGEEG T+   VL++R
Sbjct: 628 SGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELSWNCGEEGATNKSAVLQRR 687

Query: 713 LKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAFKDRKSFNWDSLKTDFGTQTTRFIA 772
           LKQIRNF+F+ ++SLGVPVLNMGDECG S+ GS   + RK F+W+ L + FGTQ T+FI+
Sbjct: 688 LKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPFDWNLLASAFGTQITQFIS 747

Query: 773 FFSSFRSRRFDLFQSRHFLKGENIDWFDNNQSPPEWEDPSCKFLAVMLR--ADKEESGSI 832
           F +S R+RR D+FQ R FLK ENI W+ N+Q+ P+WEDP+ KFLA+ ++  +++EE+ S+
Sbjct: 748 FMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEIKSESEEEETASL 807

Query: 833 TENPKPQS-DIFMAFNASDQSVSVALPEPSEGTNWFRLVDTALSFPGFFSSDGEPVPTTS 892
            E  +P+S D+F+ FNASD   SV LP   +G+ W RLVDTAL FPGFFS +GE V    
Sbjct: 808 AEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALPFPGFFSVEGETVVAEE 867

Query: 893 P----VTYEMQAHSCTLFE 902
           P    V YEM+ +SCTLFE
Sbjct: 868 PLQQLVVYEMKPYSCTLFE 876

BLAST of Carg07231 vs. Swiss-Prot
Match: sp|Q6AU80|ISOA2_ORYSJ (Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=1 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 5.7e-201
Identity = 352/715 (49.23%), Postives = 470/715 (65.73%), Query Frame = 0

Query: 205 AVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPV----GFGRGYPSPLGL 264
           A EL FD    PFYLSFLL      D+  +EI++HRKTSF VPV                
Sbjct: 105 AAELSFDPTRAPFYLSFLL-----TDASGAEIRTHRKTSFRVPVXXXXXXXXXXXXXXXX 164

Query: 265 SISGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGASNF 324
                                             LE+DLDPYI+R+GN+WHVS      +
Sbjct: 165 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXALEIDLDPYIHRTGNVWHVSLASVDGY 224

Query: 325 VSYGYRCRGSKFHENQDEAEVSRILVDPYAKIL--TASTPKTRGQGVGLPS-KFLGQICN 384
           VSY + C G           + R L+DPYAK++    S+     +GV  PS +    +  
Sbjct: 225 VSYAFCCGG-----------IRRPLLDPYAKVIGDFVSSNSVYDEGVTAPSMRCFASLAI 284

Query: 385 VPAFSWDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGV 444
            P+++W  +  P LP+EKL +YR NV  FT D+SS LP D AGTF+GL+ K+ HF+ LGV
Sbjct: 285 APSYNWGRDRHPRLPLEKLVVYRANVALFTKDRSSGLPDDAAGTFTGLSAKVEHFRSLGV 344

Query: 445 NAVLLEPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILE 504
           NA+LLEP+FPF +  GPYFP+HFFSP N+Y   G S+S++ S+K+MV+ +H NG+EV+LE
Sbjct: 345 NAILLEPVFPFHQVKGPYFPYHFFSPMNLYSSKGLSVSAIKSMKDMVRVMHRNGIEVLLE 404

Query: 505 VVFTHTA------MDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWV 564
           VVFTHTA         +++GID+S YY  N +A  +  S LNCNHP+ Q+L+LDSLR+WV
Sbjct: 405 VVFTHTAEGESECQTISMRGIDNSSYYIANGIAGCKA-SILNCNHPVTQKLILDSLRHWV 464

Query: 565 TEFHIDGFCFINASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKET 624
            +FH+DGFCFINA  L++G  GE LSRPP +EAI FDP+LS TKIIA+ W P ++   + 
Sbjct: 465 LDFHVDGFCFINAPFLVRGPGGEYLSRPPLLEAITFDPVLSMTKIIADPWSPLDISNVQF 524

Query: 625 RFPHWKRWAELNSKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNV 684
            FPHWKRWAE+N++FS D+++F + E L+S LATRLCGSGD+FS  RGPAFSFN ++RN 
Sbjct: 525 PFPHWKRWAEVNTRFSIDVRKFLKREALISDLATRLCGSGDLFST-RGPAFSFNHVSRNS 584

Query: 685 GLPLVDLVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNM 744
           GL LVDLVSFS+  L SE SWNCGEEGP+ N  VL+ RL+QIRNF+F+LFVSLGVPVLNM
Sbjct: 585 GLSLVDLVSFSNDDLLSESSWNCGEEGPSENSAVLQTRLRQIRNFLFILFVSLGVPVLNM 644

Query: 745 GDECGQSSGGSVAFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGE 804
           GDECG S+ GSV++KDR   NW  +KT F  + T FI+F ++ RSRR D+FQ R FLK E
Sbjct: 645 GDECGHSAAGSVSYKDRGPLNWRGMKTTFVKEVTGFISFLTALRSRRGDIFQRREFLKLE 704

Query: 805 NIDWFDNNQSPPEWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVA 864
           NI W+ ++   P W+DP+  FL + + A+ +E  +       + D+++ FNA+++SVS A
Sbjct: 705 NIHWYGSDLCEPGWDDPTSNFLCMHINAEVDEMAA----DSVRGDLYICFNANEESVSAA 764

Query: 865 LPEPSEGTNWFRLVDTALSFPGFFSSDGEPVPTTSP--VTYEMQAHSCTLFEAKS 905
           LP  +EG+ W RLVDT+L+FPGFF+++  P     P   +Y ++AH+C LFE+KS
Sbjct: 765 LPALAEGSVWLRLVDTSLAFPGFFATESNPKVQQVPGLSSYHVEAHTCVLFESKS 797

BLAST of Carg07231 vs. Swiss-Prot
Match: sp|B9G434|ISOA3_ORYSJ (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 6.5e-104
Identity = 256/711 (36.01%), Postives = 368/711 (51.76%), Query Frame = 0

Query: 246 VPVGFGRGYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----NDSTSEKPVLELDLD 305
           +P  +  G   PLG+S   +G +NF++FS  A SV+LCL       +      V+E  LD
Sbjct: 96  MPFKYSSGKAFPLGVS-QVEGGLNFALFSQHASSVILCLKLPGRGTEDEKGADVVEFVLD 155

Query: 306 PYINRSGNIWHVSFEG-ASNFVSYGYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPK 365
              N++G+IWHV  EG  ++ V YGYR  G +  +     + S +L+DPYAK++  S  K
Sbjct: 156 QQKNKTGDIWHVIVEGLPASGVLYGYRVGGPQGWDQGHRFDSSTVLLDPYAKLV--SGRK 215

Query: 366 TRGQGVGLPSKFLGQI-CNVPAFSWDGEVR-PNLPMEKLFIYRLNVKHFTVDKSSQLPID 425
             G      S+  G    +   F W  + R PNLP   L IY +NV+ FT D+SS L   
Sbjct: 216 YFGVAEEKSSQHFGTYDFDSSPFDWGDDYRLPNLPEADLVIYEMNVRAFTADESSGLDST 275

Query: 426 IAGTFSGLTQKLLHFKKLGVNAVLLEPIFPFDEKDGPYFP--------------FHFFSP 485
             G++ GL  K+ H  +LGVNAV L P+F +DE +   +P               +FF+P
Sbjct: 276 SRGSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNPRDHMVNTWGYSTINFFAP 335

Query: 486 TNIYVPSGAS-ISSLNSVKEMVKELHANGVEVILEVVFTHT--AMDG-----ALQGIDDS 545
            + Y  +G   +++   +K+MVKELH  G+EVIL+VV+ HT  A D      + +GID+ 
Sbjct: 336 MSRYASAGGGPVAASKELKQMVKELHKAGIEVILDVVYNHTNEADDAHPYMTSFRGIDNK 395

Query: 546 CYYFVNRVANSE------GKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLQ 605
            YY ++   N+E        + LNCNHP+V++L+LDSLR+WV E+HIDGF F  AS L +
Sbjct: 396 VYYMLDLNKNAELLNFSGCGNTLNCNHPVVKELILDSLRHWVEEYHIDGFRFDLASVLCR 455

Query: 606 GHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAELNSKFSND 665
           G  G  L  PP ++ IA D +LS+ KIIAE WD   L     RFP+W RWAE N K+ +D
Sbjct: 456 GPDGCPLDAPPLIKEIAKDAVLSRCKIIAEPWDCGGLY-LVGRFPNWDRWAEWNGKYRDD 515

Query: 666 MKEFFRGE-GLVSSLATRLCGSGDVFS-DGRGPAFSFNFIARNVGLPLVDLVSFS----- 725
           ++ F +G+ G+    ATR+ GS D++  + R P    NF+  + G  L DLVS++     
Sbjct: 516 LRRFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNFVIAHDGFTLCDLVSYNLKHND 575

Query: 726 ------SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECG 785
                         SWNCG EG T+++ VL  R +Q++NF   L +S G P++ MGDE G
Sbjct: 576 ANGEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKNFHVALMISQGTPMMLMGDEYG 635

Query: 786 QSSGGSVAFKDR----KSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGEN 845
            +  G+           +F W+ L+        RF +    FR     L + R FL   +
Sbjct: 636 HTRYGNNNSYGHDTCINNFQWEQLEQRRDGH-FRFFSEMIKFRHSNPILRRDR-FLNKND 695

Query: 846 IDWFDNNQSPPEWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVAL 904
           + W ++      WE+   KFLA  +  D    G          DI++AFNA D  V   +
Sbjct: 696 VTWHEDC-----WENQESKFLAFTVH-DHNSGG----------DIYLAFNAHDYFVDAVI 755

BLAST of Carg07231 vs. Swiss-Prot
Match: sp|Q9M0S5|ISOA3_ARATH (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 379.0 bits (972), Expect = 1.5e-103
Identity = 253/709 (35.68%), Postives = 361/709 (50.92%), Query Frame = 0

Query: 253 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----NDSTSEKPVLELDLDPYINRSG 312
           G  SPLG+S   D  INF++FS  A SV LCL       D T +  ++EL LDP +N++G
Sbjct: 88  GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 313 NIWHVSFEGAS-NFVSYGYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVG 372
           + WH+  E    N V YGYR  G    +     + S +L+DPYAK++   +  + G    
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGHS--SFGDSSQ 207

Query: 373 LPSKFLGQI-CNVPAFSWDGEVR-PNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSG 432
             ++F G        F W  + + PN+P + L IY +NV+ FT D+SS +   I G++ G
Sbjct: 208 KFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267

Query: 433 LTQKLLHFKKLGVNAVLLEPIFPFDE-----KDGP---------YFPFHFFSPTNIYVP- 492
             +K+ H + LG+NAV L P+F FDE     +  P         Y   +FF+P + Y   
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327

Query: 493 SGASISSLNSVKEMVKELHANGVEVILEVVFTHT-------AMDGALQGIDDSCYYFV-- 552
            G  I +    KEMVK LH+ G+EVIL+VV+ HT           + +GID+  YY +  
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387

Query: 553 -NRVANSEG-KSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLQGHHGELLSR 612
            N++ N  G  + LNCNHP+V +L+LDSLR+WVTE+H+DGF F  AS L +   G  LS 
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447

Query: 613 PPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAELNSKFSNDMKEFFRGE- 672
           PP + AIA D +LS+ KIIAE WD   L     +FP+W RWAE N  + +D++ F +G+ 
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWDCGGLY-LVGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507

Query: 673 GLVSSLATRLCGSGDVFS-DGRGPAFSFNFIARNVGLPLVDLVSFS-----------SSK 732
           G+  S ATR+ GS D++  + R P    NF+  + G  L DLVS++           +  
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567

Query: 733 LASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAF 792
                SWNCG EG T +  +   R +Q++NF   L +S G P++ MGDE G +  G    
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYG---- 627

Query: 793 KDRKSFNWDSLKTDF-----GTQTTRFIAFFSSFRSRRFDLFQSRHFLKGEN------ID 852
            +  S+  D+   +F       +      FFS     R     S H LK EN      I 
Sbjct: 628 -NNNSYGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFR----HSHHVLKHENFLTQGEIT 687

Query: 853 WFDNNQSPPEWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPE 904
           W ++N     W++   KFLA  L    +  G          DI++AFNA D  V   +P+
Sbjct: 688 WHEDN-----WDNSESKFLAFTLH---DGIGG--------RDIYVAFNAHDYFVKALIPQ 747

BLAST of Carg07231 vs. Swiss-Prot
Match: sp|D0TZF0|ISOA1_ORYSJ (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.9e-95
Identity = 233/692 (33.67%), Postives = 347/692 (50.14%), Query Frame = 0

Query: 253 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY--NDSTSEKPVLELDLDPYINRSGNIW 312
           G P+PLG + + DG +NF+++S+ A +  LCL+  +D  +++   E+ LDP  NR+GN+W
Sbjct: 99  GMPAPLG-ATALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLDPLFNRTGNVW 158

Query: 313 HVSFEGASNFVSYGYRCRGSKFHENQDEAEVSRILVDPYAK-ILTASTPKTRGQGVGLPS 372
           HV  EG  + + YGYR  G          +VS ++VDPYAK +++       G G     
Sbjct: 159 HVFIEGELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVISRGEYGVPGPGGDCWP 218

Query: 373 KFLGQI-CNVPAFSWDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQK 432
           +  G I      F W G++    P + L IY ++++ FT   SS   ++  GT+ G   K
Sbjct: 219 QMAGMIPLPYSTFDWQGDLPLRYPQKDLVIYEMHLRGFTKHSSSN--VEHPGTYIGAISK 278

Query: 433 LLHFKKLGVNAVLLEPIFPFDEKD----------GPYFPFHFFSPTNIYVPSG---ASIS 492
           L + K+LGVN V L P   F+E +            Y   +FFSP   Y   G       
Sbjct: 279 LDYLKELGVNCVELMPCHEFNELEYFSCSSKMNFWGYSTINFFSPMIRYSSGGIRNCGRD 338

Query: 493 SLNSVKEMVKELHANGVEVILEVVFTHTAMDG------ALQGIDDSCYYFV---NRVANS 552
           ++N  K  V+E H  G+EVI++VVF HTA         + +GID+S YY +       N 
Sbjct: 339 AINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSTYYMLAPKGEFYNY 398

Query: 553 EG-KSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLQG--------------- 612
            G  +  NCNHP+V++ ++D LRYWVTE H+DGF F  AS + +G               
Sbjct: 399 SGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYGSPVE 458

Query: 613 ----HHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAELNSKF 672
                 G  L+ PP ++ I+ DP+L   K+IAE WD   L  +  +FPHWK W+E N K+
Sbjct: 459 GDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWDAGGLY-QVGQFPHWKIWSEWNGKY 518

Query: 673 SNDMKEFFRG-EGLVSSLATRLCGSGDVF-SDGRGPAFSFNFIARNVGLPLVDLVSFSSS 732
            + +++F +G +G     A  LCGS  ++ + GR P  S NF+  + G  L DLV+++  
Sbjct: 519 RDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYNKK 578

Query: 733 KLAS-----------ELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGD 792
             +S            LSWNCGEEG  + + V   R +Q+RNF   L VS GVP+  MGD
Sbjct: 579 YNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYMGD 638

Query: 793 ECGQSSGG--SVAFKDR--KSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLK 852
           E G + GG  +    D     F WD  K +  +   RF +  + FR ++ +      F  
Sbjct: 639 EYGHTKGGNNNTYCHDHYVNYFRWD--KKEESSDLQRFCSLMTKFR-KQCESLGLADFPT 698

Query: 853 GENIDWFDNNQSPPEWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVS 882
            + + W  +    P+W + S +F+A    + K+E+         + +I++AFNAS     
Sbjct: 699 AQRLHWHGHQPGKPDWSETS-RFVAF---STKDET---------KGEIYVAFNASHLPAV 758

BLAST of Carg07231 vs. TrEMBL
Match: tr|A0A1S3BRF8|A0A1S3BRF8_CUCME (isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1)

HSP 1 Score: 1526.9 bits (3952), Expect = 0.0e+00
Identity = 744/884 (84.16%), Postives = 806/884 (91.18%), Query Frame = 0

Query: 24  MATFLSSL-IQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 83
           MATFLSSL IQPFC +N G TES KLAV+D+ TYGQK  YQSGK+DEARM AHGESKVG 
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 84  TKSLHRNVCKAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 143
            KS HRN+ KAY  SGI VGKSGQ+ G GGK++E RKVATYLFRTEFGDLV  FVGK GS
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 144 GFLVSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 203
            F V+IEISS++L S DETLLLSW VYRS+SA VT NFQS  P+ T GATETPFV+TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 204 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 263
           KF+VELEFDAKH PFYLSF+LK+P+GVDSGSSEI+SHRKTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 264 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGASNFVS 323
           SGDGSINFSIFSSTAESVVLCLYNDSTSEKP+LELDLDPYINRSGNIWH SFEGASNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 324 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSKFLGQICNVPAFS 383
           YGY+C+G K HENQD  EVSRI+VDPYAKIL  S PK+R QG+G P KFLGQI  VPAF 
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 384 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLL 443
           WDGEVRPNLPMEKLF+YRLNVK FT+DKSSQLP DIAGTFSGLT+KLLH K LGVNAVLL
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 444 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 503
           EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS++NS+KEMVKELHANGVEVI+EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 504 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFI 563
           T++DGALQGIDDS YYF NRVAN E +SALNCN+PIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 564 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAEL 623
           NAS LLQGHHGELLSRPPFVEAIAFDP+LSKTK++A+FWDPQELV KETRFPHWKRWAE+
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 624 NSKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 683
           NSKF +D+++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 684 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 743
           +S LASELSWNCGEEGPT+ ++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 744 VAFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGENIDWFDNNQSP 803
           VAF DR+SFNWD LKTDFGTQTT+FIAF SSFRSRRFDLFQSR+FLKGENIDWFDNNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 804 PEWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 863
           P+WED SCKFLAVMLRADKEE+ SITENPK +SDIFMAFNASDQS SVALPEPSEGT+WF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 864 RLVDTALSFPGFFSSDGEPVPTTSPVTYEMQAHSCTLFEAKSFN 907
           R+VDTAL FPGFFSSDGE VP T  VTYEMQAHSC LFEAKS N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

BLAST of Carg07231 vs. TrEMBL
Match: tr|A0A0A0K551|A0A0A0K551_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 SV=1)

HSP 1 Score: 1522.3 bits (3940), Expect = 0.0e+00
Identity = 736/885 (83.16%), Postives = 808/885 (91.30%), Query Frame = 0

Query: 24  MATFLSSL-IQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 83
           MATFLSSL IQPFC YN G TES KLA +D+ TYGQK  YQ GK+DEARM AHGE+KVG 
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 84  TKSLHRNVCKAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 143
            KS HRN+ K YA SGISVGKSGQ+ G GGK++E R+VATYLFRTEFGDLV  FVGK+GS
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 144 GFLVSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 203
            F V+IE+ S++L SIDE LLLSWGVYRS+SA+VTPNF+S  P+ET GATETPFV+TSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 204 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 263
           KF+VELEFDAKH PFYLSF+LKYP+GVDSGSSEI+SH+KTSFSVPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 264 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGASNFVS 323
           SGDGS+NFSIFSS+AES+VLCLYNDSTSEKP+LELDLDPYINRSGNIWH SFEGAS FVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 324 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSKFLGQICNVPAFS 383
           YGY+C+GSK HENQD  EVSRI+VDPYAKIL  S PK+ GQG+GLPSKFLGQI  VP F 
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 384 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLL 443
           WDGEV PNLPMEKLF+YRLNV+ FT+DKSSQLP DIAGTFSGLT+KLLHFK LGVNAVLL
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 444 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 503
           EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS++NS+KEMVKELHANGVEVILEVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480

Query: 504 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFI 563
           T+ +GALQGIDDS YYF NRVAN E KSALNCN+PIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 564 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAEL 623
           NAS LL+GHHGELLSRPPFVEAIAFDPLLSKTK++A+FWDPQEL  KETRFPHWKRWAE+
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600

Query: 624 NSKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 683
           NSKF +D+++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 684 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 743
           +S LASELSWNCGEEGPTSN++VLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 744 VAFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGENIDWFDNNQSP 803
           VAF D++SFNWD LKTDFGTQTT+FIAF SSFRSRRFDLFQ+R+FLKGENIDWFDNNQSP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780

Query: 804 PEWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 863
           P+WED SCKFLAVMLRADKEE+ SITENPK +S+IFM FNASDQS SVALPEP EGT+WF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840

Query: 864 RLVDTALSFPGFFSSDGEPVPTTSPVTYEMQAHSCTLFEAKSFND 908
           R+VDTAL FPGFFSSDGE VP T  VTYE+QAHSC LFEAKS ND
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 885

BLAST of Carg07231 vs. TrEMBL
Match: tr|A0A2N9GWM1|A0A2N9GWM1_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS31596 PE=4 SV=1)

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 551/889 (61.98%), Postives = 677/889 (76.15%), Query Frame = 0

Query: 24  MATFLSSL-IQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 83
           MAT   SL IQP C    G + S KL+   ++     + Y   K+D  R    GE+    
Sbjct: 1   MATLPPSLAIQP-CNLKCGASGSSKLSPACHYICRNHIRYGFEKMDLERKLICGEAAQNV 60

Query: 84  TKS--LHRNVCKAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKR 143
            KS   H N  K +A S + V +  QK  T  + E   KV+TYLF TE G  V  FV KR
Sbjct: 61  VKSPCQHLN-SKVFATSRVLVEEIEQKVSTATEAEGLEKVSTYLFWTEVGGQVKVFVRKR 120

Query: 144 GSGFLVSIEISSLKLASIDETLLLSWGVYRSNSAIVTP-NFQSILPEETIGATETPFVRT 203
              ++V+IE+SSL L S ++ L LSWG YR++S+   P + QS  P+  I  +ETPF++T
Sbjct: 121 SVKYVVNIEVSSLPLHSSEDRLTLSWGRYRADSSSFMPLDVQSSEPDTRISTSETPFIQT 180

Query: 204 SEGKFAVELEFDAKHDPFYLSFLLKYPVGVDSGSS--EIKSHRKTSFSVPVGFGRGYPSP 263
           S G+FA+ELEF+AK  PFYLSFLLK  +  DSG S  EI+SHRKT+F VPVG G GYP+P
Sbjct: 181 SAGRFALELEFEAKQFPFYLSFLLK--LQSDSGLSGLEIRSHRKTNFCVPVGLGTGYPAP 240

Query: 264 LGLSISGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGA 323
           LGLS S DGS+NF+IFS  AE V+LCLY+D+T+++P LELDLDPY+NRSG+IWH SFE A
Sbjct: 241 LGLSFSPDGSMNFAIFSRYAEGVILCLYDDTTADEPALELDLDPYVNRSGDIWHASFESA 300

Query: 324 SNFVSYGYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSKFLGQICN 383
             FVSYGYRC+GS    + ++ +  +I++DPYAKI+  S+P + G    L  K+LGQ+C 
Sbjct: 301 WTFVSYGYRCKGSLLQRSNNKLDAGQIILDPYAKIIGNSSPSSHGS--KLLQKYLGQLCK 360

Query: 384 VPAFSWDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGV 443
            PAF W  +  P LPMEKL +YRLNV+HFT  KSSQLP D+AGTFSGLT+KL HFK LG+
Sbjct: 361 EPAFDWGDDTHPYLPMEKLLVYRLNVRHFTEHKSSQLPTDVAGTFSGLTEKLQHFKDLGM 420

Query: 444 NAVLLEPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILE 503
           NAVLLEPIF FDEK+GPYFPFHFFSP N+Y PSG S+S++NS+KEMVK++HANG+EV+LE
Sbjct: 421 NAVLLEPIFSFDEKNGPYFPFHFFSPMNLYGPSGGSVSAINSMKEMVKKMHANGIEVLLE 480

Query: 504 VVFTHTAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWVTEFHID 563
           VVFTHTA  G+LQGIDD  YY+ N+V + E   ALNCN+PIVQQL+LDSLR+WVTEFH+D
Sbjct: 481 VVFTHTAAAGSLQGIDDLSYYYANKVGDLEAPKALNCNYPIVQQLILDSLRHWVTEFHVD 540

Query: 564 GFCFINASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWK 623
           GFCFINASSLL+G HGE LSRPP +EAIAFDPLLSKTKIIA+ WDP +++PKE RFPHWK
Sbjct: 541 GFCFINASSLLRGFHGEYLSRPPLIEAIAFDPLLSKTKIIADCWDPHDMLPKENRFPHWK 600

Query: 624 RWAELNSKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVD 683
           RWAE+++KF ND++ F+RGEG++S LATRLCGSGD+FSDGRGPAFSFNFIARN GL LVD
Sbjct: 601 RWAEIDTKFCNDVRNFWRGEGVLSDLATRLCGSGDIFSDGRGPAFSFNFIARNFGLTLVD 660

Query: 684 LVSFSSSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQ 743
           LVSFSS  LAS+LSWNCGEEGPT N  VLE+RLKQIRNF+FVL++SLGVP+LNMGDECGQ
Sbjct: 661 LVSFSSDALASQLSWNCGEEGPTDNTTVLERRLKQIRNFLFVLYISLGVPILNMGDECGQ 720

Query: 744 SSGGSVAFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGENIDWFD 803
           SSGGS A+ DRK F+W++LKT FG QT++FI+F S FR RR DL Q R+FLK ENIDW  
Sbjct: 721 SSGGSPAYTDRKPFDWNALKTGFGIQTSQFISFLSLFRMRRSDLLQKRNFLKEENIDWHG 780

Query: 804 NNQSPPEWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSE 863
            +Q PP+WEDPS +FLA+ LRADK ES   TE+   + D+F+AFNA+D S SV LP P E
Sbjct: 781 RDQFPPKWEDPSRRFLAMTLRADKVESPLSTESSHLRGDLFIAFNAADHSESVILPTPPE 840

Query: 864 GTNWFRLVDTALSFPGFFSSDGEPVPTTSP--VTYEMQAHSCTLFEAKS 905
           G +W  +VDTAL FPGFFS++GEP+P      V Y+M++HSC LFEA S
Sbjct: 841 GMSWLCMVDTALPFPGFFSTNGEPLPKQKAELVAYKMKSHSCALFEASS 883

BLAST of Carg07231 vs. TrEMBL
Match: tr|M5VNY8|M5VNY8_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_8G244800 PE=4 SV=1)

HSP 1 Score: 1090.1 bits (2818), Expect = 0.0e+00
Identity = 537/886 (60.61%), Postives = 666/886 (75.17%), Query Frame = 0

Query: 24  MATFLSSLIQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGFT 83
           MAT   S+    C +N G TES KL   +   +  K  +   KLD  R     E      
Sbjct: 1   MATLPLSIAIQACCFNCGITESSKLTATNGFRHRDKAMHGFVKLDAERKLVFREVVQNIN 60

Query: 84  KSLHR-NVCKAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 143
           ++L R +  K YA S +SV    Q+F TG +TEE  KV+TYLFRTE GD+V  FV KR +
Sbjct: 61  ENLPRDHNLKVYAKSQVSVEPMEQRFSTGTETEEIDKVSTYLFRTEIGDVVNVFVRKRNA 120

Query: 144 GFLVSIEISSLKLASIDETLLLSWGVYRSNSAIVTP-NFQSILPEETIGATETPFVRTSE 203
            + V+IE+ S  L+S D  L+L WG+YR++S+   P +F+S  P +T    ETP ++TS 
Sbjct: 121 KYTVNIEVPSSHLSSNDRRLVLRWGMYRADSSCFVPLDFKSSTPNDTTTTLETPLIQTSS 180

Query: 204 GKFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLS 263
           G+F +ELEF+AK  PFY SF+L  P   +    EI+SHRKT+F VPVGFGRGYP PLGL+
Sbjct: 181 GRFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPGPLGLT 240

Query: 264 ISGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGASNFV 323
            S DGS+NF+IFS  AESV LCLY+++T+EKP LELDLDPY+NRSG+IWHVSF  A +FV
Sbjct: 241 FSNDGSMNFAIFSRNAESVALCLYDNTTAEKPALELDLDPYVNRSGDIWHVSFGSAWSFV 300

Query: 324 SYGYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSKFLGQICNVPAF 383
           SYGY+ +G+    N++  +   +L+DPYAK++  S P   G G+    K+LG++C  PAF
Sbjct: 301 SYGYKFKGNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGL----KYLGRLCEEPAF 360

Query: 384 SWDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVL 443
            W G+VRP+L MEKL +YRLNV  FT  KSSQLP +I G+FSGLT+KL HFK LGVNAVL
Sbjct: 361 DWAGDVRPDLSMEKLVVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDLGVNAVL 420

Query: 444 LEPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFT 503
           LEPIFPFDE+ GPYFP HFFSP + + PS   +S++NS+KEMV++ HANG+EV+LEVVFT
Sbjct: 421 LEPIFPFDEQKGPYFPHHFFSPMDCFGPSRGPVSAVNSMKEMVRKFHANGIEVLLEVVFT 480

Query: 504 HTAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCF 563
           HTA   ALQGID S YY VN V + E ++ALNCN+PIVQQL+LDSLRYWVTEFH+DGF F
Sbjct: 481 HTAEGEALQGIDISSYYHVNEVEDLEARNALNCNYPIVQQLVLDSLRYWVTEFHVDGFFF 540

Query: 564 INASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAE 623
           INASSLL+G +GE LSRPP VEAIAFDPLLSKTKIIA+ WDP  + PKET FPHW+RWAE
Sbjct: 541 INASSLLRGFNGEYLSRPPLVEAIAFDPLLSKTKIIADCWDPHGMAPKETHFPHWRRWAE 600

Query: 624 LNSKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF 683
           +N+KF ND++ F RGEGL+S LATRLCG+GD+FSDGRGPAF+FNFI+RN GLPLVDLVSF
Sbjct: 601 VNTKFCNDVRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFAFNFISRNSGLPLVDLVSF 660

Query: 684 SSSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGG 743
           S  +LASELSWNCGEEGPT+   VLE+RLKQIRNF+F+LFVSLGVPVLNMGDECGQS+GG
Sbjct: 661 SGVELASELSWNCGEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECGQSTGG 720

Query: 744 SVAFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGENIDWFDNNQS 803
           S A+ DRK+F+W++L+T F TQTT+FIAF SSFR RR DL Q R+FLK ENI W++++Q+
Sbjct: 721 SPAYSDRKAFDWNALETGFATQTTQFIAFLSSFRKRRSDLLQKRNFLKEENIGWYESDQT 780

Query: 804 PPEWEDPSCKFLAVMLRADKEESGSI-TENPKPQSDIFMAFNASDQSVSVALPEPSEGTN 863
           PP WEDPS KFLA+ L+AD++E      E+     D+F+AF+A+D S +V LP P EG  
Sbjct: 781 PPRWEDPSRKFLAMRLKADEDEVNQPGDESSHSWGDLFVAFSAADHSETVVLPPPLEGMG 840

Query: 864 WFRLVDTALSFPGFFSSDGEPV--PTTSPVTYEMQAHSCTLFEAKS 905
           W RLVDTAL FPGFFS+DGEPV         YEM++HSC LFEA+S
Sbjct: 841 WRRLVDTALPFPGFFSTDGEPVVEQIVGLFAYEMKSHSCALFEARS 882

BLAST of Carg07231 vs. TrEMBL
Match: tr|A0A2P6PW47|A0A2P6PW47_ROSCH (Putative isoamylase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr6g0291581 PE=4 SV=1)

HSP 1 Score: 1074.7 bits (2778), Expect = 1.8e-310
Identity = 534/885 (60.34%), Postives = 659/885 (74.46%), Query Frame = 0

Query: 24  MATFLSSLIQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGFT 83
           MA    S+    C +  G TES KL    ++    K      KLD  R    G     FT
Sbjct: 1   MAMLPLSITLQACCWACGTTESSKLTATTHYRDRNKTTLGLVKLDAERKLVFGGFVQSFT 60

Query: 84  KSLHRNV-CKAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 143
           +SL R    +  A SG+S+    QKF TG  TEE  KV+TYLFRTE GDLV  FV     
Sbjct: 61  QSLPRGCHSRVQATSGVSIEPMEQKFTTG--TEETDKVSTYLFRTENGDLVNVFVRNNTV 120

Query: 144 GFLVSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 203
            + V +E+SSL+L+S  + L+LSWG+YR++S+       S++P + I   ETPF+ TS G
Sbjct: 121 NYSVYVEVSSLQLSSSADRLVLSWGMYRADSS-------SLMPSDFI---ETPFIETSSG 180

Query: 204 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 263
            F +ELEF+AK  PFYLSF++K     +    EI+SHRKT+F VPVGFGRGYP+PLGLS 
Sbjct: 181 SFTLELEFEAKQTPFYLSFIVKSLADANLSGLEIRSHRKTNFCVPVGFGRGYPTPLGLSF 240

Query: 264 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGASNFVS 323
           S DGSINF+IFS  AESVVLCL +D T++KP LELDLDPY+NRSG+IWHVSFE A  FVS
Sbjct: 241 SSDGSINFAIFSRNAESVVLCLDDDPTAQKPALELDLDPYVNRSGDIWHVSFESAWTFVS 300

Query: 324 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSKFLGQICNVPAFS 383
           YGYR +G+    N +  +   IL+DPYAK++  S P  +G G    SK +G++C  P F 
Sbjct: 301 YGYRFKGTLL-RNTNSFDEGDILLDPYAKVIDESIPNNKGTG----SKLVGRLCEEPVFD 360

Query: 384 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVLL 443
           WDG+VRP+LP+EKL +YRLN+  FT  KSS+LP ++AGTFSGLT+KL  FK LGVNA+LL
Sbjct: 361 WDGDVRPHLPIEKLMVYRLNLTRFTEHKSSKLPTNVAGTFSGLTEKLDQFKDLGVNAILL 420

Query: 444 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 503
           EPIFPFDE+ GPYFP HFFSP N + PSG  I+++NS+KEMVKE HANG+EV+LEVVFTH
Sbjct: 421 EPIFPFDEEKGPYFPCHFFSPMNCFGPSGGPIATINSMKEMVKEFHANGIEVLLEVVFTH 480

Query: 504 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCFI 563
           TA    LQGID S YY  +   + E +++LNCNHPIVQQ++LDSLRYWVTEFH+DGFCFI
Sbjct: 481 TAAGEVLQGIDTSSYYHASTAKDLEARNSLNCNHPIVQQMVLDSLRYWVTEFHVDGFCFI 540

Query: 564 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAEL 623
           NASSLL+G +GE  SRPP VEAIAFDPLL+KTK IA+ WDP E+VPKETRFPHWKRWAE+
Sbjct: 541 NASSLLRGVNGEYQSRPPLVEAIAFDPLLTKTKFIADCWDPHEMVPKETRFPHWKRWAEV 600

Query: 624 NSKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 683
           N++F ND++ F RGEGL+S LATRLCG+GD+FSDGRGPAFSFNFI+RN GLPLVDLVSFS
Sbjct: 601 NARFCNDVRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFSFNFISRNSGLPLVDLVSFS 660

Query: 684 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 743
            S+LASELSWNCGEEGPTS   VLE+RLKQIRNF+F+L++SLGVPVLNMGDECGQSSGGS
Sbjct: 661 GSELASELSWNCGEEGPTSKTAVLERRLKQIRNFLFILYLSLGVPVLNMGDECGQSSGGS 720

Query: 744 VAFKDRKSFNWDSLKTDFGTQTTRFIAFFSSFRSRRFDLFQSRHFLKGENIDWFDNNQSP 803
            A+ DRKSF+W++++T F TQT +FIA+ SSFR RR DL Q +H+ K ENIDW+ ++QS 
Sbjct: 721 PAYSDRKSFDWNAMETGFATQTRQFIAYLSSFRRRRSDLLQKKHYFKEENIDWYGSDQSL 780

Query: 804 PEWEDPSCKFLAVMLRADKE--ESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTN 863
           P WEDP CKFLA+ L+AD++  E+    E    + D+F+AF+A++QS +V LP P EG  
Sbjct: 781 PRWEDPLCKFLAMRLKADQDEVENQLTNEYADLRGDLFVAFSAAEQSETVILPPPPEGMA 840

Query: 864 WFRLVDTALSFPGFFSSDGEPV--PTTSPVTYEMQAHSCTLFEAK 904
           W RLVDTAL FPGFFS DGEP+        +Y M++HSC LFEA+
Sbjct: 841 WRRLVDTALPFPGFFSIDGEPIIEQMEGLFSYAMKSHSCALFEAR 868