Lsi08G014660 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi08G014660
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Calcium-binding EF-hand family protein, putative
Location	chr08 : 22797390 .. 22801316 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTTGTCAAGATCACAACTGAAGTTGTCAAGGGATGAGATTAAGGAGATCTTACAAGACCATGATATTGATGGAGATGGCACCCTCTGCAAGAAAGAGGTGGTGCAAGCCCTGCAATCTTTAGGCACTTGGACTGCGCATCAGAAAGCTCACTATGGTGTTGCGTTTGCTGACGAAGATGGTGATGGTGTCATCGATGAACATGAGTTGGACAAACTCGTCGACTATGTCATGAGGTTTCAGGCCCCAAAGGCCCCCAAGGGCCCCAAGCCCCCAAAAAAGCATTGTGACGATGGCAAATCTATTGTCTAATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAGTTTACACATTACGTATTACACATTACAAACATTTGAAAGTCTATACCTGCAAAGTTTACACATTATGTTAGTTGTTACGCTGTTGAATAGGGATTAAAAAATGTAGCATGCCATCTTTGACATGCTTAACTTTAACGTAATGACCGAATTTTCAACAACAGGGAGATCTATAAATGGGGTGTGCGGGAGAATATAGTTGTTTTGGGTGTGTGTGGAGAATAATATTCTTTTATTTGCAACTTGATCTACAATTAATTTATTTATTTATTTTTTTTTTGGAGAAAGTACAAAAGTCTTCCTTTATGTTAATTATTTATACCTCCTTTTAATTGGCACTATTTCATGTTTTTTTTTTTTTTTGTTTTGTTTACATGCACTTTTAGTGTCGAGGTGTGATGAGGGTGCTAAGATATGTCCTAGTTGATATATCCAGGTGCACCTACTAATCTTCTACCTTCGTATCTTGTTAAAAAAAAGAAAAACATTAACAAACTATTAACATTTTCATTCAAACTTTCAATTGCCCATTATTTAGGTATTTTGCTTCTTTTTTTTTTTTTTTTTCAAGTTTTGTTTTTGAAAGATATAAATCATGAATGTAAAATTTGATAACAATTTTTTTCCCTAAAAAACTAAGTACTTTTTTTTAGTGGTTTTAGGTTTTAGTTTTTATAAAAAACCAAAAATCAGTCAGTTTGTTACATACTTTTAGTTTTGTTTTTTTTTTTAAAAAAAAAAAGAATGAAGATCAAATCACAAATGTTTACATACTTAGATTTTATAATAAAAATAAGTATTTAGAATTTGATTCGATTATAATGAGATTATTTTGATGGTGTTTATGGCAAATTACAAGCCACAATCTTAATAAGAAATCTATTGGTATTGTTTACTTTCATATGGAATTGCAACGAAGTATAGATGATAATCAAAATGTCATTTTTATATTTTTAATTAATTGGAGTATCATTGTCTATTTTAATTTAACTATTAATTTTTATTTACTTGTTCAAATTTTTATACTTGAACAAAAAATTATATTAGAAAATTTTAAATTTTAAAAATATAATAGTCGTTATCTTAAATCTCACCCATCTGTTTTCAATATTTGTAAAATAAAAACATTATAATTTTCCAAACAAAATCTAAGATAATTAGAATTTTTTTTATTATTTATTTTTTTGTAATTAATGGATGATATGTAGAAAGAAATGTTTGTACAATATATATTCTGGTTCATGATAGACCACTTAAATAGCTAAATAATTCGTGAAAAACGATTCTTCTAGTTCGGTAGGCTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTGAAAAACAATAATAATAGTAATAACAACAACTTGATTCGGATACTTTATGGTTGAGTTTGAGTCTCCTTTTACTAGCCTAAATCGTGGCTAGCTTAAGGATATTATTTAAACACTTAGATTTAACGCATTATACAATTTAATTTTGAATATTTTGCCGGTTGAATTTTAACTAATTAATTGATCCATTCACGAGTAACTTTCTTTTCCAACTTATTAATTGATTAGTTTCACCGTTCACAGTATTTAGTTGCTTATGGTTTAACTCGTTAAGCTGCAGATGAGTTTTAATCTCATTTATTGATAAGATTTTTCTAGTTGACTTGACATTTGGTTGAGGGCAGATATGACCTCTTTTGTCTTAATTTTGATTGTATCTCACTATCTTGTTCTTCTAAAAGAAAATTTTAATTAATTATATTAAATTCCAAAGTTCAAATTTAATGTTAAATTCATCCAAATTGGAAGATTGAATTAAAATTGGAGTAAAATTACAATATTATGTTTAGTACATCCACGAATGTCGGAGATGCGATCTGTTATTACTACAGTGTGTTATATTAAAAAAAATTCAAATTTTGTATTTAGTACATATTTAAGACCTAATGCATACAAAATCAAAATTTAAGTTTACAATTTGTAAAGTAGATGGACTAAAATAAATACAGGTTTTAAAATTTAATGACTAGACTTGTTATTTAATTTTTTTTAATTATATAGATTTTTATGTACTACACAATTAAATGGTAATTCATATAATTGCTTGTTTTAATTTACTAAACAATTAAATATTCACTACACACATGCACGGTCAATATCAATATAATAGTAGAATTAAAAAAAAAAAAAAAAAAGGAAATATTTTCAAGGAATTGCATGAGGTCAGATTAATTTGGACTTGGATATGATATCTCTTGATGGATGCTGTACGGGAAACTATATTAGATTTGCTATCTAGTTTCGTCTGGTCTCGTAACCATGACTACTTTTTCTAGTTACGAACGGGTCACGTAGATATGCAATAAAGGCCTGATTAACATGAACAAACATTTTTGGATGGAATTCTCGTTTGTTGTCGATACGAAGATATCAATTGGGCTATATTAAACTAGCATGCTCAGTAACCCATATGTTGTATTTTATGATATGTGGCTACTTCTTAAAATATGAAGTGTCATCGGTTTATTAATTTAAAGTGATTTTCAACTTTTGATTATTTTTCTTTCTATTAAGTGCCAAAAATATATAATATTTTTACTTTATATATATATATATATATATTTAATTTAAAAAACTAAAGTGTCGATTTTAAAATGGTAGGTATCAAAATATACAGCAAATTGAAACCTATCCAAACTAGTGTATTTAGTCACAAAAATAATCAAATCAAATATATATATAAAAAGCTAATGAATTATTTGGTGTGGAGTACTTATTTTGGTCCATGACAAAAGCCACTTAAATTGCTAAAATTTGTGTTACCGATGAGAACAAGCACAATTAGTAGTGTACTTATCTTCTTTTTTCTTTCTTTAATTTTTTTTTCTTTTAACATAAATTAAATTAAAGGTACATATTTGAGGATATAAGAGAACCAAGATTAATCAATCGCATCTGACAAAGATAGGAAGTTCAAAATGTTCAAAACGTTCTATATTCGCCGAGGCTTACTAATCAAGATAAGCATACACGAGATACACCATCAAGATGGAACCTAAAAATCTATGCTTTTTAAGAACCCTTTCATGCCTCACATTTTCTTCTCAACAGTTCTTCAATCGCTGCAAAATCGTGTTGGTTTCGTCTGTGTTCGTAGCGATAAAATGACTCGTAAACATGACAAGAAGAAACCAGGGTTGGAAAAAGAAGAAATGAAAGCAATTTTTAGGGAGCATGATTTCAATGGCGACGGTCGCTTGAGCATCAAAGAATTATCGAGAGTGTTCGGGTCGCTCGGTGCATTCTTCCCTCTCTACCGTGCAACTTGTGGCTTGTTCATTGCCGATGAGGACGGCGATGGATTTATCACTGAGCAAGAACTAGAGAAAGTGATAGATTATGCTATTAAATGTAAGTATAACATAATATAG

mRNA sequence

ATGGCGTTGTCAAGATCACAACTGAAGTTGTCAAGGGATGAGATTAAGGAGATCTTACAAGACCATGATATTGATGGAGATGGCACCCTCTGCAAGAAAGAGGTGGTGCAAGCCCTGCAATCTTTAGGCACTTGGACTGCGCATCAGAAAGCTCACTATGGTGTTGCGTTTGCTGACGAAGATGTTCTTCAATCGCTGCAAAATCGTGTTGGTTTCGTCTGTGTTCGTAGCGATAAAATGACTCGTAAACATGACAAGAAGAAACCAGGGTTGGAAAAAGAAGAAATGAAAGCAATTTTTAGGGAGCATGATTTCAATGGCGACGGTCGCTTGAGCATCAAAGAATTATCGAGAGTGTTCGGGTCGCTCGGTGCATTCTTCCCTCTCTACCGTGCAACTTGTGGCTTGTTCATTGCCGATGAGGACGGCGATGGATTTATCACTGAGCAAGAACTAGAGAAAGTGATAGATTATGCTATTAAATGTAAGTATAACATAATATAG

Coding sequence (CDS)

ATGGCGTTGTCAAGATCACAACTGAAGTTGTCAAGGGATGAGATTAAGGAGATCTTACAAGACCATGATATTGATGGAGATGGCACCCTCTGCAAGAAAGAGGTGGTGCAAGCCCTGCAATCTTTAGGCACTTGGACTGCGCATCAGAAAGCTCACTATGGTGTTGCGTTTGCTGACGAAGATGTTCTTCAATCGCTGCAAAATCGTGTTGGTTTCGTCTGTGTTCGTAGCGATAAAATGACTCGTAAACATGACAAGAAGAAACCAGGGTTGGAAAAAGAAGAAATGAAAGCAATTTTTAGGGAGCATGATTTCAATGGCGACGGTCGCTTGAGCATCAAAGAATTATCGAGAGTGTTCGGGTCGCTCGGTGCATTCTTCCCTCTCTACCGTGCAACTTGTGGCTTGTTCATTGCCGATGAGGACGGCGATGGATTTATCACTGAGCAAGAACTAGAGAAAGTGATAGATTATGCTATTAAATGTAAGTATAACATAATATAG

Protein sequence

MALSRSQLKLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQNRVGFVCVRSDKMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYRATCGLFIADEDGDGFITEQELEKVIDYAIKCKYNII

Homology

[Close]

BLAST of Lsi08G014660 vs. Swiss-Prot
Match: CETN4_MOUSE (Centrin-4 OS=Mus musculus GN=Cetn4 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.3e-07
Identity = 45/145 (31.03%), Postives = 69/145 (47.59%), Query Frame = 1


Query: 12  RDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQNRVG 71
           + EIKE     DIDG GT+  KE+  A+++LG     ++    +A  D++   ++     
Sbjct: 26  KQEIKEAFDLFDIDGSGTIDLKELKIAMRALGFEPKKEEVKQLIAEIDKEGTGTICFE-D 85

Query: 72  FVCVRSDKMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYR 131
           F  + S KM+ K        EKEE+   F+  D +  G +S+  + RV   LG       
Sbjct: 86  FFAIMSVKMSEKD-------EKEEILKAFKLFDDDATGSISLNNIKRVAKELGENLTEDE 145

Query: 132 ATCGLFIADEDGDGFITEQELEKVI 157
               L  AD DGDG I E+E  K++
Sbjct: 146 LQEMLDEADRDGDGEINEEEFLKMM 162

[Close]

BLAST of Lsi08G014660 vs. Swiss-Prot
Match: CML8_ORYSJ (Probable calcium-binding protein CML8 OS=Oryza sativa subsp. japonica GN=CML8 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.3e-07
Identity = 42/145 (28.97%), Postives = 73/145 (50.34%), Query Frame = 1


Query: 12  RDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQNRVG 71
           R EIKE     D DG GT+  KE+  A+++LG     ++ H  +A  D+D   ++ +   
Sbjct: 45  RKEIKEAFDLFDTDGSGTIDPKELNVAMRALGFEMTPEQIHQMIAEVDKDGSGTI-DFDE 104

Query: 72  FVCVRSDKMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYR 131
           FV + +DKM  +         +EE+   F+  D + +G++S  ++ R+    G  F L  
Sbjct: 105 FVHMMTDKMGERD-------AREELNKAFKIIDKDNNGKISDVDIQRLAIETGEPFTLDE 164

Query: 132 ATCGLFIADEDGDGFITEQELEKVI 157
               +  ADE+GDG +  +E  K++
Sbjct: 165 VREMIEAADENGDGEVDHEEFLKMM 181

[Close]

BLAST of Lsi08G014660 vs. Swiss-Prot
Match: CML7_ARATH (Calmodulin-like protein 7 OS=Arabidopsis thaliana GN=CML7 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.3e-07
Identity = 45/149 (30.20%), Postives = 71/149 (47.65%), Query Frame = 1


Query: 14  EIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQ--NRVG 73
           E+K + Q  D +GDGT+  KE+ + L+SLG +             D+++ Q ++  +  G
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIY-----------IPDKELTQMIEKIDVNG 64

Query: 74  FVCVRSDKMTR--KHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPL 133
             CV  D+     K    +   E+E+MK  F   D NGDG +++ EL  V  SLG     
Sbjct: 65  DGCVDIDEFGELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGK 124

Query: 134 YRATCGLFI--ADEDGDGFITEQELEKVI 157
               C   I   D DGDG +  +E  +++
Sbjct: 125 TLDDCKKMIKKVDVDGDGRVNYKEFRQMM 142

[Close]

BLAST of Lsi08G014660 vs. Swiss-Prot
Match: CETN1_HUMAN (Centrin-1 OS=Homo sapiens GN=CETN1 PE=1 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 3.0e-07
Identity = 40/145 (27.59%), Postives = 68/145 (46.90%), Query Frame = 1


Query: 12  RDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQNRVG 71
           + E++E     D+DG GT+  KE+  A+++LG     ++    ++  D +    +     
Sbjct: 30  KQEVREAFDLFDVDGSGTIDAKELKVAMRALGFEPRKEEMKKMISEVDREGTGKISFN-D 89

Query: 72  FVCVRSDKMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYR 131
           F+ V + KM+ K         KEE+   FR  D +  G++S K L RV   LG       
Sbjct: 90  FLAVMTQKMSEKD-------TKEEILKAFRLFDDDETGKISFKNLKRVANELGENLTDEE 149

Query: 132 ATCGLFIADEDGDGFITEQELEKVI 157
               +  AD DGDG + E+E  +++
Sbjct: 150 LQEMIDEADRDGDGEVNEEEFLRIM 166

[Close]

BLAST of Lsi08G014660 vs. Swiss-Prot
Match: CML14_ORYSJ (Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica GN=CML14 PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.1e-06
Identity = 43/154 (27.92%), Postives = 75/154 (48.70%), Query Frame = 1


Query: 3   LSRSQLKLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDV 62
           L  SQLK    +++E+ +  D++GDG+L + E+   L+SLG      + H  +A  D + 
Sbjct: 18  LRGSQLK----QLRELFRRFDMNGDGSLTQLELAALLRSLGLRPTGDEVHALLAGMDANG 77

Query: 63  LQSLQNRVGFVCVRSDKMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGS 122
             S++       +     T+ H      +++ ++  +FR  D +G+G +S  EL+R    
Sbjct: 78  NGSVEFDELAAAIAPVLTTQTH-----LVDQAQLLEVFRAFDRDGNGFISAAELARSMAR 137

Query: 123 LGAFFPLYRATCGLFIADEDGDGFITEQELEKVI 157
           LG        T  +  AD DGDG I+ +E   V+
Sbjct: 138 LGQPLTFEELTRMMRDADTDGDGVISFKEFAAVM 162

[Close]

BLAST of Lsi08G014660 vs. TrEMBL
Match: A0A0A0LKW4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 9.3e-32
Identity = 70/91 (76.92%), Postives = 81/91 (89.01%), Query Frame = 1


Query: 80  MTRK-HDKKKPG--LEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYRATCGL 139
           MTR+ +DKKK G  L+KEEMK +FREHDFNGDGRLSIKELSR FG  GAFFPLYRA  GL
Sbjct: 1   MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60

Query: 140 FIADEDGDGFITEQELEKVIDYAIKCKYNII 168
           F+AD+DGDGFI+EQEL+KV+DYAIKCKYN++
Sbjct: 61  FLADDDGDGFISEQELDKVVDYAIKCKYNLL 91

[Close]

BLAST of Lsi08G014660 vs. TrEMBL
Match: A0A0A0LRC3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359900 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 9.3e-16
Identity = 42/61 (68.85%), Postives = 53/61 (86.89%), Query Frame = 1


Query: 1  MALSRSQLKLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADE 60
          MA SRSQLKL+RDE++EIL++HD+DGDG L K+EV+QAL S+G   + QKAHYGVAFAD+
Sbjct: 1  MATSRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMGVMMSFQKAHYGVAFADK 60

Query: 61 D 62
          D
Sbjct: 61 D 61

[Close]

BLAST of Lsi08G014660 vs. TrEMBL
Match: A0A0A0LNG3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357860 PE=4 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 2.7e-15
Identity = 40/61 (65.57%), Postives = 55/61 (90.16%), Query Frame = 1


Query: 1  MALSRSQLKLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADE 60
          MA+SRSQLKL+RDE++EIL++HD+DGDG+L K+EV+QAL S+G+  + QKAHYGV+ AD+
Sbjct: 1  MAMSRSQLKLTRDEVREILEEHDVDGDGSLTKQEVMQALNSMGSMMSFQKAHYGVSHADK 60

Query: 61 D 62
          D
Sbjct: 61 D 61

[Close]

BLAST of Lsi08G014660 vs. TrEMBL
Match: A0A0A0LRC1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 2.7e-15
Identity = 39/74 (52.70%), Postives = 56/74 (75.68%), Query Frame = 1


Query: 91  LEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYRATCGLFIADEDGDGFITEQ 150
           L +E++K IFREHD +GDGRLS+ E+ + FG  G+  P Y+A  G+  ADE+GDGFI E+
Sbjct: 10  LSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFIEEE 69

Query: 151 ELEKVIDYAIKCKY 165
           EL+K++DYA K ++
Sbjct: 70  ELDKLVDYAQKFQH 83

[Close]

BLAST of Lsi08G014660 vs. TrEMBL
Match: A0A0A0LRC8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359950 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.5e-13
Identity = 50/157 (31.85%), Postives = 84/157 (53.50%), Query Frame = 1


Query: 11  SRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTA----HQKAHYGVAFADEDVLQSL 70
           +++E  E+++ +D +GDG L K+E+   L+     T        +   V   +E++ Q +
Sbjct: 10  AKNEANELIKKYDRNGDGVLTKEELQSFLRDARGSTQLNNIRNVSSSTVPKTNENLAQKV 69

Query: 71  QNRVGFVCVRS--DKMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLG 130
            +       +   DK      +    L +E++K IF+ HD N DG LSI+EL++ F S+G
Sbjct: 70  SSNTCITPKKQLLDKAQGSSKQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTKAFSSMG 129

Query: 131 AFFPLYRATCGLFIADEDGDGFITEQELEKVIDYAIK 162
           +  P  +A   +  AD D DG ++E EL+K+IDYA K
Sbjct: 130 SIVPFCKARYAMAYADVDEDGLVSEAELDKLIDYAHK 166

[Close]

BLAST of Lsi08G014660 vs. TAIR10
Match: AT1G05990.1 (AT1G05990.1 EF hand calcium-binding protein family)

HSP 1 Score: 57.0 bits (136), Expect = 1.3e-08
Identity = 45/149 (30.20%), Postives = 71/149 (47.65%), Query Frame = 1


Query: 14  EIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQ--NRVG 73
           E+K + Q  D +GDGT+  KE+ + L+SLG +             D+++ Q ++  +  G
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIY-----------IPDKELTQMIEKIDVNG 64

Query: 74  FVCVRSDKMTR--KHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPL 133
             CV  D+     K    +   E+E+MK  F   D NGDG +++ EL  V  SLG     
Sbjct: 65  DGCVDIDEFGELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGK 124

Query: 134 YRATCGLFI--ADEDGDGFITEQELEKVI 157
               C   I   D DGDG +  +E  +++
Sbjct: 125 TLDDCKKMIKKVDVDGDGRVNYKEFRQMM 142

[Close]

BLAST of Lsi08G014660 vs. TAIR10
Match: AT3G59440.1 (AT3G59440.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 54.7 bits (130), Expect = 6.4e-08
Identity = 40/142 (28.17%), Postives = 70/142 (49.30%), Query Frame = 1


Query: 14  EIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQ--NRVG 73
           ++K + Q  D +GDG + K+E+  +L++LG           +   D+D++Q +Q  +  G
Sbjct: 51  DLKRVFQMFDKNGDGRITKEELNDSLENLG-----------IFMPDKDLIQMIQKMDANG 110

Query: 74  FVCVRSDKMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYR 133
             CV  ++    +       E+ +M+  F   D +GDG ++++EL+ V  SLG       
Sbjct: 111 DGCVDINEFESLYGSIVEEKEEGDMRDAFNVFDQDGDGFITVEELNSVMTSLGLKQGKTL 170

Query: 134 ATCGLFI--ADEDGDGFITEQE 152
             C   I   DEDGDG +  +E
Sbjct: 171 ECCKEMIMQVDEDGDGRVNYKE 181

[Close]

BLAST of Lsi08G014660 vs. TAIR10
Match: AT2G43290.1 (AT2G43290.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 52.8 bits (125), Expect = 2.4e-07
Identity = 43/153 (28.10%), Postives = 70/153 (45.75%), Query Frame = 1


Query: 9   KLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLG--------TWTAHQKAHYGVAFADE 68
           ++   E+K + Q  D +GDG + K+E+  +L++LG        T   H+    G    D 
Sbjct: 60  RIDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDI 119

Query: 69  DVLQSLQNRVGFVCVRSDKMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVF 128
           D  +SL + +         +   H+  +   E+E+MK  F   D +GDG ++++EL  V 
Sbjct: 120 DEFESLYSSI---------VDEHHNDGET--EEEDMKDAFNVFDQDGDGFITVEELKSVM 179

Query: 129 GSLGAFFPLYRATCGLFI--ADEDGDGFITEQE 152
            SLG         C   I   D DGDG +  +E
Sbjct: 180 ASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKE 201

[Close]

BLAST of Lsi08G014660 vs. TAIR10
Match: AT4G12860.1 (AT4G12860.1 EF hand calcium-binding protein family)

HSP 1 Score: 50.1 bits (118), Expect = 1.6e-06
Identity = 38/149 (25.50%), Postives = 68/149 (45.64%), Query Frame = 1


Query: 10  LSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQNR 69
           + R E+  + Q  D +GDG + K E+    +S+G      + +  +A  D +      + 
Sbjct: 1   MDRGELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNG-DGAMDI 60

Query: 70  VGFVCVRSDKMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPL 129
             F  +  + +  K        E+E+M+  FR  D NGDG ++ +EL  V  S+G     
Sbjct: 61  DEFGSLYQEMVEEKE-------EEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGR 120

Query: 130 YRATCGLFIA--DEDGDGFITEQELEKVI 157
               C   I+  D DGDG +  +E ++++
Sbjct: 121 TLEDCKKMISKVDVDGDGMVNFKEFKQMM 141

[Close]

BLAST of Lsi08G014660 vs. TAIR10
Match: AT4G03290.1 (AT4G03290.1 EF hand calcium-binding protein family)

HSP 1 Score: 49.3 bits (116), Expect = 2.7e-06
Identity = 42/153 (27.45%), Postives = 69/153 (45.10%), Query Frame = 1


Query: 14  EIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQ--NRVG 73
           E+  + Q  D DGDG +  KE+ ++ ++LG           +   ++++ Q +Q  +  G
Sbjct: 5   ELNRVFQMFDKDGDGKITTKELNESFKNLG-----------IIIPEDELTQIIQKIDVNG 64

Query: 74  FVCVRSD------KMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGA 133
             CV  +      K     D+ + G  +E+MK  F   D NGDG +++ EL  V  SLG 
Sbjct: 65  DGCVDIEEFGELYKTIMVEDEDEVG--EEDMKEAFNVFDRNGDGFITVDELKAVLSSLGL 124

Query: 134 FFPLYRATCGLFI--ADEDGDGFITEQELEKVI 157
                   C   I   D DGDG +   E  +++
Sbjct: 125 KQGKTLEECRKMIMQVDVDGDGRVNYMEFRQMM 144

[Close]

BLAST of Lsi08G014660 vs. NCBI nr
Match: gi|700207396|gb|KGN62515.1| (hypothetical protein Csa_2G358860 [Cucumis sativus])

HSP 1 Score: 144.8 bits (364), Expect = 1.3e-31
Identity = 70/91 (76.92%), Postives = 81/91 (89.01%), Query Frame = 1


Query: 80  MTRK-HDKKKPG--LEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYRATCGL 139
           MTR+ +DKKK G  L+KEEMK +FREHDFNGDGRLSIKELSR FG  GAFFPLYRA  GL
Sbjct: 1   MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60

Query: 140 FIADEDGDGFITEQELEKVIDYAIKCKYNII 168
           F+AD+DGDGFI+EQEL+KV+DYAIKCKYN++
Sbjct: 61  FLADDDGDGFISEQELDKVVDYAIKCKYNLL 91

[Close]

BLAST of Lsi08G014660 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 132.9 bits (333), Expect = 5.2e-28
Identity = 66/85 (77.65%), Postives = 75/85 (88.24%), Query Frame = 1


Query: 80  MTRK-HDKKKPG--LEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYRATCGL 139
           MTR+ +DKKK G  L+KEEMK +FREHDFNGDGRLSIKELSR FG  GAFFPLYRA  GL
Sbjct: 1   MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60

Query: 140 FIADEDGDGFITEQELEKVIDYAIK 162
           F+AD+DGDGFI+EQEL+KV+DYAIK
Sbjct: 61  FLADDDGDGFISEQELDKVVDYAIK 85

[Close]

BLAST of Lsi08G014660 vs. NCBI nr
Match: gi|700207402|gb|KGN62521.1| (hypothetical protein Csa_2G359900 [Cucumis sativus])

HSP 1 Score: 91.7 bits (226), Expect = 1.3e-15
Identity = 42/61 (68.85%), Postives = 53/61 (86.89%), Query Frame = 1


Query: 1  MALSRSQLKLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADE 60
          MA SRSQLKL+RDE++EIL++HD+DGDG L K+EV+QAL S+G   + QKAHYGVAFAD+
Sbjct: 1  MATSRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMGVMMSFQKAHYGVAFADK 60

Query: 61 D 62
          D
Sbjct: 61 D 61

[Close]

BLAST of Lsi08G014660 vs. NCBI nr
Match: gi|700207395|gb|KGN62514.1| (hypothetical protein Csa_2G357860 [Cucumis sativus])

HSP 1 Score: 90.1 bits (222), Expect = 3.9e-15
Identity = 40/61 (65.57%), Postives = 55/61 (90.16%), Query Frame = 1


Query: 1  MALSRSQLKLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADE 60
          MA+SRSQLKL+RDE++EIL++HD+DGDG+L K+EV+QAL S+G+  + QKAHYGV+ AD+
Sbjct: 1  MAMSRSQLKLTRDEVREILEEHDVDGDGSLTKQEVMQALNSMGSMMSFQKAHYGVSHADK 60

Query: 61 D 62
          D
Sbjct: 61 D 61

[Close]

BLAST of Lsi08G014660 vs. NCBI nr
Match: gi|700207397|gb|KGN62516.1| (hypothetical protein Csa_2G358870 [Cucumis sativus])

HSP 1 Score: 90.1 bits (222), Expect = 3.9e-15
Identity = 39/74 (52.70%), Postives = 56/74 (75.68%), Query Frame = 1


Query: 91  LEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYRATCGLFIADEDGDGFITEQ 150
           L +E++K IFREHD +GDGRLS+ E+ + FG  G+  P Y+A  G+  ADE+GDGFI E+
Sbjct: 10  LSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFIEEE 69

Query: 151 ELEKVIDYAIKCKY 165
           EL+K++DYA K ++
Sbjct: 70  ELDKLVDYAQKFQH 83

The following BLAST results are available for this feature:

BLAST of Lsi08G014660 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CETN4_MOUSE	2.3e-07	31.03	Centrin-4 OS=Mus musculus GN=Cetn4 PE=1 SV=1	[more]
CML8_ORYSJ	2.3e-07	28.97	Probable calcium-binding protein CML8 OS=Oryza sativa subsp. japonica GN=CML8 PE...	[more]
CML7_ARATH	2.3e-07	30.20	Calmodulin-like protein 7 OS=Arabidopsis thaliana GN=CML7 PE=2 SV=1	[more]
CETN1_HUMAN	3.0e-07	27.59	Centrin-1 OS=Homo sapiens GN=CETN1 PE=1 SV=1	[more]
CML14_ORYSJ	1.1e-06	27.92	Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica GN=CML14 ...	[more]

BLAST of Lsi08G014660 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKW4_CUCSA	9.3e-32	76.92	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1	[more]
A0A0A0LRC3_CUCSA	9.3e-16	68.85	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359900 PE=4 SV=1	[more]
A0A0A0LNG3_CUCSA	2.7e-15	65.57	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357860 PE=4 SV=1	[more]
A0A0A0LRC1_CUCSA	2.7e-15	52.70	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1	[more]
A0A0A0LRC8_CUCSA	2.5e-13	31.85	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359950 PE=4 SV=1	[more]

BLAST of Lsi08G014660 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G05990.1	1.3e-08	30.20	EF hand calcium-binding protein family	[more]
AT3G59440.1	6.4e-08	28.17	Calcium-binding EF-hand family protein	[more]
AT2G43290.1	2.4e-07	28.10	Calcium-binding EF-hand family protein	[more]
AT4G12860.1	1.6e-06	25.50	EF hand calcium-binding protein family	[more]
AT4G03290.1	2.7e-06	27.45	EF hand calcium-binding protein family	[more]

BLAST of Lsi08G014660 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700207396\|gb\|KGN62515.1\|	1.3e-31	76.92	hypothetical protein Csa_2G358860 [Cucumis sativus]	[more]
gi\|449470198\|ref\|XP_004152805.1\|	5.2e-28	77.65	PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus]	[more]
gi\|700207402\|gb\|KGN62521.1\|	1.3e-15	68.85	hypothetical protein Csa_2G359900 [Cucumis sativus]	[more]
gi\|700207395\|gb\|KGN62514.1\|	3.9e-15	65.57	hypothetical protein Csa_2G357860 [Cucumis sativus]	[more]
gi\|700207397\|gb\|KGN62516.1\|	3.9e-15	52.70	hypothetical protein Csa_2G358870 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR018247	EF_Hand_1_Ca_BS
IPR011992	EF-hand-dom_pair
IPR002048	EF_hand_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G014660.1	Lsi08G014660.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 139..155 score: 0.
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 13..118 score: 2.
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 95..123 score: 7.1E-4coord: 14..42 score: 3.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 10..45 score: 13.091coord: 91..126 score: 14.151coord: 140..162 score: 8
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 79..105 score: 1.4E-8coord: 106..155 score: 1.9E-11coord: 11..61 score: 2.
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 3..39 score: 1.23E-19coord: 74..156 score: 1.23
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 140..152 score: -coord: 23..35 score: -coord: 104..116 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 76..160 score: 2.0
None	No IPR available	PANTHER	PTHR10891:SF592	CALCIUM-BINDING PROTEIN CML38-RELATED	coord: 76..160 score: 2.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi08G014660	Cla022567	Watermelon (97103) v1	lsiwmB465
Lsi08G014660	MELO3C010868	Melon (DHL92) v3.5.1	lsimeB469
Lsi08G014660	MELO3C016745.2	Melon (DHL92) v3.6.1	lsimedB507
Lsi08G014660	Cla97C05G101420	Watermelon (97103) v2	lsiwmbB435
Lsi08G014660	Cp4.1LG07g05370	Cucurbita pepo (Zucchini)	cpelsiB685
Lsi08G014660	CsGy2G019080	Cucumber (Gy14) v2	cgyblsiB129
Lsi08G014660	CsaV3_2G027870	Cucumber (Chinese Long) v3	cuclsiB155

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi08G014660	Melon (DHL92) v3.6.1	lsimedB485
Lsi08G014660	Melon (DHL92) v3.6.1	lsimedB489
Lsi08G014660	Silver-seed gourd	carlsiB164
Lsi08G014660	Silver-seed gourd	carlsiB710
Lsi08G014660	Cucumber (Chinese Long) v3	cuclsiB336
Lsi08G014660	Cucumber (Chinese Long) v3	cuclsiB571
Lsi08G014660	Watermelon (97103) v2	lsiwmbB444
Lsi08G014660	Wax gourd	lsiwgoB591
Lsi08G014660	Wax gourd	lsiwgoB627
Lsi08G014660	Bottle gourd (USVL1VR-Ls)	lsilsiB148
Lsi08G014660	Cucumber (Gy14) v1	cgylsiB370
Lsi08G014660	Cucumber (Gy14) v1	cgylsiB379
Lsi08G014660	Cucurbita maxima (Rimu)	cmalsiB032
Lsi08G014660	Cucurbita maxima (Rimu)	cmalsiB153
Lsi08G014660	Cucurbita maxima (Rimu)	cmalsiB184
Lsi08G014660	Cucurbita maxima (Rimu)	cmalsiB456
Lsi08G014660	Cucurbita maxima (Rimu)	cmalsiB498
Lsi08G014660	Cucurbita maxima (Rimu)	cmalsiB739
Lsi08G014660	Cucurbita moschata (Rifu)	cmolsiB020
Lsi08G014660	Cucurbita moschata (Rifu)	cmolsiB140
Lsi08G014660	Cucurbita moschata (Rifu)	cmolsiB175
Lsi08G014660	Cucurbita moschata (Rifu)	cmolsiB442
Lsi08G014660	Cucurbita moschata (Rifu)	cmolsiB482
Lsi08G014660	Cucurbita moschata (Rifu)	cmolsiB731
Lsi08G014660	Cucurbita pepo (Zucchini)	cpelsiB100
Lsi08G014660	Cucurbita pepo (Zucchini)	cpelsiB477
Lsi08G014660	Cucurbita pepo (Zucchini)	cpelsiB644
Lsi08G014660	Wild cucumber (PI 183967)	cpilsiB141
Lsi08G014660	Wild cucumber (PI 183967)	cpilsiB316
Lsi08G014660	Wild cucumber (PI 183967)	cpilsiB552
Lsi08G014660	Cucumber (Chinese Long) v2	culsiB142
Lsi08G014660	Cucumber (Chinese Long) v2	culsiB317
Lsi08G014660	Cucumber (Chinese Long) v2	culsiB537
Lsi08G014660	Melon (DHL92) v3.5.1	lsimeB466
Lsi08G014660	Melon (DHL92) v3.5.1	lsimeB488
Lsi08G014660	Watermelon (Charleston Gray)	lsiwcgB428
Lsi08G014660	Watermelon (Charleston Gray)	lsiwcgB431
Lsi08G014660	Watermelon (Charleston Gray)	lsiwcgB438
Lsi08G014660	Watermelon (97103) v1	lsiwmB480
Lsi08G014660	Watermelon (97103) v1	lsiwmB492
Lsi08G014660	Cucumber (Gy14) v2	cgyblsiB289
Lsi08G014660	Cucumber (Gy14) v2	cgyblsiB505