Lsi08G010940 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi08G010940
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionUbiquitin-conjugating enzyme E2
Locationchr08 : 19545344 .. 19550820 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTACGTCTGTGTTATGTGGTGATGGGTAACAGCATGAAGTATTTGGATTTTCTCCGAAGATCTTATATTTTTCTGGATTCATCTGCATTTTTTTCCCTATATAATTGTAATATTATATGGTTTCTCTCAAGCCAGCCTTTTGTTATAACCCTGTATGATAGTGGATTTCTACCTCATACTTACCTATAATTATAGATGACTAATTGCTTTTGTATCTTTATGGTTATCGGGTGATTTCAATTAGCTTCATGCATCTTTGTTACTAAACAGCTATTGATACTTTTACTTCTTAGGCTTTAAAAACGTTGAAGATATTATTGTTGTAATAACAAAACTATAGTTTGTAAGTTTTAGTTTGTTAGTTGTTGAATAGTTTGTTTGTTATTTTGATTTGTTAAATTTCTTTAAACTTGTACTCACACTTTCCTTATAAATAAAGGTTATCTCCCCTCATTTAGAGGTAGAGATTCATTTGATTCAATTCTAGAAATTGGTATTACACCATTTATAGAAATAGTTAATCTTGTTAAGTGATAATAGTAGGGATGTTGAAGACAAAAATTTATTGGCAACTGACAGGGGCTTCTTTATTTATTTATTTATTTAACTTTTATCTAATTATTATTATTATGTACAATCACACACATTTAGGATCTGAAAATTGAGTCTTACACCTCATACCTCCTCAAGGTTTATATCTTGCCTCTTTAAGAGGAAAAGCCTCAAGATGCCTTTAGCCTATTAAAACTTTGATTTTGTGTAGGCCTCAAGAGCAAGGTTGTTCAAGGAGTACAAAGAGGTTCAAAGAGAAAAGGTAGCAGATCCAGATATTCAACTGGTTTGTGACGATTCCAATATTTTTAAATGGACAGCCCTAATCAAGGTAAGCCTCTTGATGATTCAGATGAAAAATTGATTTCAGAGTTGTTTCTTACCATGATTGATATGCACTTTATTTAGGGACCGTCAGAGACTCCTTTTGAAGGTGGTGTTTTTCAACTTGCCTTCTCCGTACCTGAGCAATACCCTTTGCAACCTCCTCAAGTGCGGTTCTTAACGAAAATTTTCCATCCAAATGTTCATTTTAAGGTAGAAGTCACCCGGATCTTTGTTCTAGATTATGCTAACTATGAGCCCTTCTGTCACAATTGTTAATGTTATTGTTTTTTATGAACTATACTTGGCTAGTATCTGCTCTATATACCGAAATGCCCTACGTGAACTGATGAAGTTACAACCACATTACCTCTAGTAAACTTTGAGAGCCCCCAAATCCCGAATGAGGGAACCCTAAGAAAGCCCACCAACTCCAACTACCTACAGACCATGGCCAATCTATGCTAGATCTGACTAACTTTCCACCAGCAAGATTGTGGTAAGGATTATAAAGAGGTGATCATGATTCATGGGGAGGAAAAGCAACAATATGGGAGGGGGTGTCGTTGTCCAAGGGAGCGTAACTAAATACTGTTGAGCCTCCATGCATATGGGAGTAGGGGGAGAATATATAGAAGGTAATACACGGGCAGGGGAGAGGAATATACAAAAAGAAATTTAGTGGTATTTCATGTGTGGGAGGCCTTGGATTTTCAAATCATCTTGTGGATTCTAGGGAGGCACAACAAGAAATTGAGTAGATGATATCCCTTCTGTTGAAAGGTACAACAGGAGTTGCAATGATGAAATAAGCCAATTTGATGAAACAACCGGTATAATCGTTATAAAATAATTGATGTGGTGTTGCAAACTCTAGGGATATGGGAGTGAACTTAAAAGTTGAAGAACCCTATGGGAGGAGGCAGAGTTGCAATTAAGGTGGATATTTGTATGGGGGAGTTTGACAATGACCTCTCCTTCAAACGTAAAATGTATGCTTGTCAAAAGGATGCTAGTGCTATGATTGAATCTGCCTCGTGGGGAGAAGGTTTGTTTTCATGGTTTGCTGGGGTATGTGCGATCTTGTGGATTTTGTGGGGTGAGCGGAATAGTAAAACTTTTAGAGGGGTGGATAAAGACCCTAGTGAGGTTTGGTCTCTTGTTCGCTTTCATGTTTCTTTGGGGGTTTCCGTTTTGAAGACCTTTTGTAACTACTCTATTGGCGTTATTTTGCATAGTTGGAGTCCCTTCCTGTAGAGGGAGGTTGTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTTTTTTTTTTTTTTTTTTTGTGTGTGTGTGTGAGCATGGCTTTTTGTACGGCCGTGTATTCTTTCATTTCATTCTCAATAAAAGTTGTTTCTATAAAATAAATAAATATATATACACACACATACATATAGATGGGATTGGGAGTGGGAAGCTAGATATGCTGATTTTTTATGGAGAGGATCCAAAAGCGTAGATATATTTTGTGGACTGCTTTCATATGAGAGGAATTCTTGTTGCCGAGCAATTCTGTGCATTAGGGATGGACTAGTGTTGGTGAATGGCGATCATTCACGTTTGTTTGAACCCTTTTGTGACTGTAGTCTTATTTTTTTACGACCTTACTTGAACAGGATCTTTTCTATAGGTTGTACAACTGTGTCTTTGAGTTCTAAGGAGACTGTAGTCTTATTTTACGGTGAACCTTATTTTTAATACTTTTCATTTCTGTCATTAAACATATAAACATAACTCTTTTCAGTGGTTGAATGGAACCACACTTTATACTTATCGCTGTGCCTTCGTGCAGACGGGTGAGATCTGCCTAGATATATTGAAGAATGCGTGGAGCCCAGCATGGACTCTGCAATCTGTTTGTAGGGCTATAATTGCTTTGATGGCTCATCCTGAACCTGATAGCCCGCTAAACTGTGATTCTGGTGAGTTTACATGCTTGCTTATTTGAATCTTTGGATGGTGTGATAACTGGTAATGTAGCAAAGTAGTTAAAGAACTCATTTAATAATGATTTTGTTTTTGAAATTTATACATGTTTTCTTCTATATTCCATACCATGGTTTTCACATTTTCTAGAAAAAATACTTGAATTCTTGCCAATTCTAAAAACAAAAATAAAATTTTGAAACTAAGACTGAAGCAAGTTGATCGGGTAATGTTCAGTTATATATTTTGAAAACAAATCAACTGAATATATTTTAGTAAATGAGATATTAGGAGACCTCAATGGGATGGTCTTCTTGTAAATCCTTTGTGTTGGGGAGGGGACGCCTGTCCCTTTGGCCTTTTTTTTCTTTTTTTTTTTTTTTGTTAAGGGAGATATTAGGGGACCTCACTCTTTGCAATAGGTTCATGACCTACCCGACTAATCTTTGGGAAAGTTTGGCCCTTCCTAAGCCAACTTCTAGAGGCACTAGGATTAGATTTTAAATGATTAAGACCCCATTTGTTAACTATCTGGTTTTTTTGCTTATGAAAATTGTACTTCTTTTTTTCTTTCTAGATGAGTTACTCTTTTCATTGTTAATTGGTTTTGAGATGGAACCCCATATTATCGAATAAAACTCATAGATGGAATTAGTACGGGCCTAAATATGTAGACTTGAACTTAAGACCATGGAGAGAATCTCCCAACATTTCAAATACGAGGGTTTGCCCTTGGAGGTAAATCGACTGAAGCATGTTGCTCGGTTTCGTAAACTATTTCCAGATCAAACTGGGTGACATATAAAGCATAGCATTTTCCCGAGGCAGATGCCAGTGACCTTTAATTCTTTTAATTATTGTGTGGGTGTTTACAGGAAACCTCCTGCGTTCGGGCGACGTCCGAGGATATCAATCAATGGCAAGGATGTACACAAGGCTTGCTGCCATGCCCAAAAAGGGCTGATTTGACATTGCTTCCTCTTTGCCAAACATTTGAATTTGACGTTGCTGCTGTCACCAAGGGAGATTGCAGAATGTGTTATTGATCACACCAACAAATTAAATATGATTATCCAGACATCTTATGTAAAGTAGTAAAGCACATTCCATCTTCTGTGTGTTCACTTTTATTTGTTGGTTCACTAGATGGATTGCCTCGTGTTTCCCTTTTCTCATCATTTTAATTGTTCTATGTGTTGATAGTTCTGTCTTCAATATAAGAAATCAATACTTATTTAGATTTCTTTTTTAATTTTAATGTATCTTTTTTTTTAATAAATTAAAAAATGATGTTGAAAATAATGTATTGTTGGAGGTGCTAATAAAAATAGGGGTCTTTGTACAGTATTGAAATATATTGAATTTTGAATAAATCGTCTTTTAG

mRNA sequence

ATGTACGGACCGTCAGAGACTCCTTTTGAAGGTGGTGTTTTTCAACTTGCCTTCTCCGTACCTGAGCAATACCCTTTGCAACCTCCTCAAGTGCGGTTCTTAACGAAAATTTTCCATCCAAATGTTCATTTTAAGTGGTTGAATGGAACCACACTTTATACTTATCGCTGTGCCTTCGTGCAGACGGGTGAGATCTGCCTAGATATATTGAAGAATGCGTGGAGCCCAGCATGGACTCTGCAATCTGTTTGTAGGGCTATAATTGCTTTGATGGCTCATCCTGAACCTGATAGCCCGCTAAACTGTGATTCTGGAAACCTCCTGCGTTCGGGCGACGTCCGAGGATATCAATCAATGGCAAGGATGTACACAAGGCTTGCTGCCATGCCCAAAAAGGGCTGATTTGACATTGCTTCCTCTTTGCCAAACATTTGAATTTGACGTTGCTGCTGTCACCAAGGGAGATTGCAGAATGTGTTATTGATCACACCAACAAATTAAATATGATTATCCAGACATCTTATGTAAAGTAGTAAAGCACATTCCATCTTCTGTGTGTTCACTTTTATTTGTTGGTTCACTAGATGGATTGCCTCGTGTTTCCCTTTTCTCATCATTTTAATTGTTCTATGTGTTGATAGTTCTGTCTTCAATATAAGAAATCAATACTTATTTAGATTTCTTTTTTAATTTTAATGTATCTTTTTTTTTAATAAATTAAAAAATGATGTTGAAAATAATGTATTGTTGGAGGTGCTAATAAAAATAGGGGTCTTTGTACAGTATTGAAATATATTGAATTTTGAATAAATCGTCTTTTAG

Coding sequence (CDS)

ATGTACGGACCGTCAGAGACTCCTTTTGAAGGTGGTGTTTTTCAACTTGCCTTCTCCGTACCTGAGCAATACCCTTTGCAACCTCCTCAAGTGCGGTTCTTAACGAAAATTTTCCATCCAAATGTTCATTTTAAGTGGTTGAATGGAACCACACTTTATACTTATCGCTGTGCCTTCGTGCAGACGGGTGAGATCTGCCTAGATATATTGAAGAATGCGTGGAGCCCAGCATGGACTCTGCAATCTGTTTGTAGGGCTATAATTGCTTTGATGGCTCATCCTGAACCTGATAGCCCGCTAAACTGTGATTCTGGAAACCTCCTGCGTTCGGGCGACGTCCGAGGATATCAATCAATGGCAAGGATGTACACAAGGCTTGCTGCCATGCCCAAAAAGGGCTGA

Protein sequence

MYGPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
BLAST of Lsi08G010940 vs. Swiss-Prot
Match: PEX4_ARATH (Protein PEROXIN-4 OS=Arabidopsis thaliana GN=PEX4 PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 1.3e-58
Identity = 110/131 (83.97%), Postives = 113/131 (86.26%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETP+EGGVFQLAFSVPE YPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPYEGGVFQLAFSVPEPYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRG+ SMA+M
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGFNSMAQM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

BLAST of Lsi08G010940 vs. Swiss-Prot
Match: UBCX_DICDI (Ubiquitin-conjugating enzyme E2 pex4 OS=Dictyostelium discoideum GN=pex4 PE=3 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 8.1e-40
Identity = 75/124 (60.48%), Postives = 94/124 (75.81%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GP +TP+E G F+L  SVP  YPLQPP ++F+TKIFHPN+HFK                T
Sbjct: 39  GPPDTPYENGKFELDISVPTNYPLQPPTIKFVTKIFHPNIHFK----------------T 98

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLD+LK +WS  +TLQSVCR+IIAL++ PE DSPLNCD+GNL+R+GDV+G+ S+ARM
Sbjct: 99  GEICLDLLKTSWSAIYTLQSVCRSIIALLSLPEADSPLNCDAGNLIRNGDVKGHDSLARM 146

Query: 123 YTRL 127
           YTRL
Sbjct: 159 YTRL 146

BLAST of Lsi08G010940 vs. Swiss-Prot
Match: UBC16_SCHPO (Ubiquitin-conjugating enzyme E2 16 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=ubc16 PE=3 SV=2)

HSP 1 Score: 122.5 bits (306), Expect = 3.5e-27
Identity = 62/129 (48.06%), Postives = 78/129 (60.47%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GP+ETP+EGG + L   V E YP+ PP V F TKI HPN+   W NG             
Sbjct: 43  GPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNI--SWTNG------------- 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
            E+C+DILK  WSPAW+LQS C AII+L+++ +  SPLN D+  LLR+GD   Y S+ R 
Sbjct: 103 -EVCMDILKTHWSPAWSLQSACLAIISLLSNYDASSPLNVDAAKLLRTGDKTAYNSLVRC 155

Query: 123 YTRLAAMPK 132
            T L A  K
Sbjct: 163 TTYLYAKGK 155

BLAST of Lsi08G010940 vs. Swiss-Prot
Match: UBCX_PICAN (Ubiquitin-conjugating enzyme E2-21 kDa OS=Pichia angusta GN=PEX4 PE=3 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 3.1e-23
Identity = 56/143 (39.16%), Postives = 75/143 (52.45%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLT----------------------KIFHP 62
           GP ++PFE  +++L   +P  YPL PP+++F+                       K+ HP
Sbjct: 50  GPDQSPFEDALWKLEIDIPTNYPLDPPKIKFVVFGEEKIRQLQRKTSSGARKVCYKMPHP 109

Query: 63  NVHFKWLNGTTLYTYRCAFVQTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPL 122
           NV+FK                TGEICLDIL+  WSPAWTLQS   AI+ L+A+PEP SPL
Sbjct: 110 NVNFK----------------TGEICLDILQQKWSPAWTLQSALVAIVVLLANPEPLSPL 169

Query: 123 NCDSGNLLRSGDVRGYQSMARMY 124
           N D  NLL+  D   Y+ +   Y
Sbjct: 170 NIDMANLLKCDDTTAYKDLVHYY 176

BLAST of Lsi08G010940 vs. Swiss-Prot
Match: UBE2N_RAT (Ubiquitin-conjugating enzyme E2 N OS=Rattus norvegicus GN=Ube2n PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 3.4e-22
Identity = 54/127 (42.52%), Postives = 77/127 (60.63%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GP ++PFEGG F+L   +PE+YP+  P+VRF+TKI+HPNV                  + 
Sbjct: 41  GPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVD-----------------KL 100

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           G ICLDILK+ WSPA  +++V  +I AL++ P PD PL  D     +S + +  ++ AR 
Sbjct: 101 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKSNEAQAIET-ARA 149

Query: 123 YTRLAAM 130
           +TRL AM
Sbjct: 161 WTRLYAM 149

BLAST of Lsi08G010940 vs. TrEMBL
Match: I1K9A6_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_06G082900 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 4.2e-59
Identity = 115/131 (87.79%), Postives = 115/131 (87.79%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

BLAST of Lsi08G010940 vs. TrEMBL
Match: A0A0B2QDV8_GLYSO (Protein PEROXIN-4 OS=Glycine soja GN=glysoja_025083 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 4.2e-59
Identity = 115/131 (87.79%), Postives = 115/131 (87.79%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

BLAST of Lsi08G010940 vs. TrEMBL
Match: I3SC14_LOTJA (Uncharacterized protein OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 4.2e-59
Identity = 115/131 (87.79%), Postives = 115/131 (87.79%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

BLAST of Lsi08G010940 vs. TrEMBL
Match: A0A151TDR4_CAJCA (Putative ubiquitin carrier protein E2 21 OS=Cajanus cajan GN=KK1_011407 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 4.2e-59
Identity = 115/131 (87.79%), Postives = 115/131 (87.79%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 253 GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK----------------T 312

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM
Sbjct: 313 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 367

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 373 YTRLAAMPKKG 367

BLAST of Lsi08G010940 vs. TrEMBL
Match: A0A068V6B4_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00017256001 PE=3 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 5.4e-59
Identity = 114/131 (87.02%), Postives = 115/131 (87.79%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGD+RGYQSMARM
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDIRGYQSMARM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

BLAST of Lsi08G010940 vs. TAIR10
Match: AT5G25760.1 (AT5G25760.1 peroxin4)

HSP 1 Score: 226.9 bits (577), Expect = 7.5e-60
Identity = 110/131 (83.97%), Postives = 113/131 (86.26%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETP+EGGVFQLAFSVPE YPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPYEGGVFQLAFSVPEPYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRG+ SMA+M
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGFNSMAQM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

BLAST of Lsi08G010940 vs. TAIR10
Match: AT1G16890.3 (AT1G16890.3 ubiquitin-conjugating enzyme 36)

HSP 1 Score: 101.7 bits (252), Expect = 3.6e-22
Identity = 51/126 (40.48%), Postives = 78/126 (61.90%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GP+++P+EGGVF+L   +PE+YP+  P+VRFLTKI+HPN+                  + 
Sbjct: 52  GPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNID-----------------KL 111

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           G ICLDILK+ WSPA  +++V  +I AL++ P PD PL+ +     +S +    ++ A+ 
Sbjct: 112 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVET-AKE 159

Query: 123 YTRLAA 129
           +TRL A
Sbjct: 172 WTRLYA 159

BLAST of Lsi08G010940 vs. TAIR10
Match: AT5G53300.1 (AT5G53300.1 ubiquitin-conjugating enzyme 10)

HSP 1 Score: 101.3 bits (251), Expect = 4.8e-22
Identity = 51/127 (40.16%), Postives = 71/127 (55.91%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSE+P+ GGVF +    P  YP +PP+V F TK+FHPN++                   
Sbjct: 39  GPSESPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNIN-----------------SN 98

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           G ICLDILK  WSPA T+  V  +I +L+  P PD PL  +  ++ ++ D   Y+S AR 
Sbjct: 99  GSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKT-DKNKYESTARS 147

Query: 123 YTRLAAM 130
           +T+  AM
Sbjct: 159 WTQKYAM 147

BLAST of Lsi08G010940 vs. TAIR10
Match: AT4G27960.2 (AT4G27960.2 ubiquitin conjugating enzyme 9)

HSP 1 Score: 100.5 bits (249), Expect = 8.1e-22
Identity = 50/127 (39.37%), Postives = 71/127 (55.91%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPS++P+ GGVF +    P  YP +PP+V F TK+FHPN++                   
Sbjct: 69  GPSDSPYSGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNIN-----------------SN 128

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           G ICLDILK  WSPA T+  V  +I +L+  P PD PL  +  ++ ++ D   Y+S AR 
Sbjct: 129 GSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKT-DKNKYESTART 177

Query: 123 YTRLAAM 130
           +T+  AM
Sbjct: 189 WTQKYAM 177

BLAST of Lsi08G010940 vs. TAIR10
Match: AT1G64230.4 (AT1G64230.4 ubiquitin-conjugating enzyme 28)

HSP 1 Score: 100.1 bits (248), Expect = 1.1e-21
Identity = 51/127 (40.16%), Postives = 71/127 (55.91%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPS++P+ GGVF +    P  YP +PP+V F TK+FHPNV+                   
Sbjct: 81  GPSDSPYSGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNVN-----------------SN 140

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           G ICLDILK  WSPA T+  V  +I +L+  P PD PL  +  ++ ++ D   Y+S AR 
Sbjct: 141 GSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKT-DRAKYESTARS 189

Query: 123 YTRLAAM 130
           +T+  AM
Sbjct: 201 WTQKYAM 189

BLAST of Lsi08G010940 vs. NCBI nr
Match: gi|734366760|gb|KHN18193.1| (Protein PEROXIN-4 [Glycine soja])

HSP 1 Score: 235.3 bits (599), Expect = 6.0e-59
Identity = 115/131 (87.79%), Postives = 115/131 (87.79%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

BLAST of Lsi08G010940 vs. NCBI nr
Match: gi|1012259300|ref|XP_015945666.1| (PREDICTED: protein PEROXIN-4 [Arachis duranensis])

HSP 1 Score: 235.3 bits (599), Expect = 6.0e-59
Identity = 115/131 (87.79%), Postives = 115/131 (87.79%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

BLAST of Lsi08G010940 vs. NCBI nr
Match: gi|388499480|gb|AFK37806.1| (unknown [Lotus japonicus])

HSP 1 Score: 235.3 bits (599), Expect = 6.0e-59
Identity = 115/131 (87.79%), Postives = 115/131 (87.79%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

BLAST of Lsi08G010940 vs. NCBI nr
Match: gi|1012353991|gb|KYP65178.1| (putative ubiquitin carrier protein E2 21 [Cajanus cajan])

HSP 1 Score: 235.3 bits (599), Expect = 6.0e-59
Identity = 115/131 (87.79%), Postives = 115/131 (87.79%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 253 GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK----------------T 312

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM
Sbjct: 313 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 367

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 373 YTRLAAMPKKG 367

BLAST of Lsi08G010940 vs. NCBI nr
Match: gi|661880151|emb|CDP16171.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 235.0 bits (598), Expect = 7.8e-59
Identity = 114/131 (87.02%), Postives = 115/131 (87.79%), Query Frame = 1

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGD+RGYQSMARM
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDIRGYQSMARM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PEX4_ARATH1.3e-5883.97Protein PEROXIN-4 OS=Arabidopsis thaliana GN=PEX4 PE=1 SV=1[more]
UBCX_DICDI8.1e-4060.48Ubiquitin-conjugating enzyme E2 pex4 OS=Dictyostelium discoideum GN=pex4 PE=3 SV... [more]
UBC16_SCHPO3.5e-2748.06Ubiquitin-conjugating enzyme E2 16 OS=Schizosaccharomyces pombe (strain 972 / AT... [more]
UBCX_PICAN3.1e-2339.16Ubiquitin-conjugating enzyme E2-21 kDa OS=Pichia angusta GN=PEX4 PE=3 SV=1[more]
UBE2N_RAT3.4e-2242.52Ubiquitin-conjugating enzyme E2 N OS=Rattus norvegicus GN=Ube2n PE=1 SV=1[more]
Match NameE-valueIdentityDescription
I1K9A6_SOYBN4.2e-5987.79Uncharacterized protein OS=Glycine max GN=GLYMA_06G082900 PE=3 SV=1[more]
A0A0B2QDV8_GLYSO4.2e-5987.79Protein PEROXIN-4 OS=Glycine soja GN=glysoja_025083 PE=3 SV=1[more]
I3SC14_LOTJA4.2e-5987.79Uncharacterized protein OS=Lotus japonicus PE=2 SV=1[more]
A0A151TDR4_CAJCA4.2e-5987.79Putative ubiquitin carrier protein E2 21 OS=Cajanus cajan GN=KK1_011407 PE=3 SV=... [more]
A0A068V6B4_COFCA5.4e-5987.02Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00017256001 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G25760.17.5e-6083.97 peroxin4[more]
AT1G16890.33.6e-2240.48 ubiquitin-conjugating enzyme 36[more]
AT5G53300.14.8e-2240.16 ubiquitin-conjugating enzyme 10[more]
AT4G27960.28.1e-2239.37 ubiquitin conjugating enzyme 9[more]
AT1G64230.41.1e-2140.16 ubiquitin-conjugating enzyme 28[more]
Match NameE-valueIdentityDescription
gi|734366760|gb|KHN18193.1|6.0e-5987.79Protein PEROXIN-4 [Glycine soja][more]
gi|1012259300|ref|XP_015945666.1|6.0e-5987.79PREDICTED: protein PEROXIN-4 [Arachis duranensis][more]
gi|388499480|gb|AFK37806.1|6.0e-5987.79unknown [Lotus japonicus][more]
gi|1012353991|gb|KYP65178.1|6.0e-5987.79putative ubiquitin carrier protein E2 21 [Cajanus cajan][more]
gi|661880151|emb|CDP16171.1|7.8e-5987.02unnamed protein product [Coffea canephora][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR016135UBQ-conjugating_enzyme/RWD
IPR000608UBQ-conjugat_E2
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006635 fatty acid beta-oxidation
biological_process GO:0016558 protein import into peroxisome matrix
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006661 phosphatidylinositol biosynthetic process
biological_process GO:0006886 intracellular protein transport
biological_process GO:0008150 biological_process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005622 intracellular
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0016874 ligase activity
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0031625 ubiquitin protein ligase binding
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G010940.1Lsi08G010940.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 59..123
score: 1.2E-15coord: 2..43
score: 7.7
IPR000608Ubiquitin-conjugating enzyme E2PROFILEPS50127UBIQUITIN_CONJUGAT_2coord: 1..109
score: 30
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3DG3DSA:3.10.110.10coord: 3..129
score: 1.0
IPR016135Ubiquitin-conjugating enzyme/RWD-likeunknownSSF54495UBC-likecoord: 3..129
score: 3.81
NoneNo IPR availablePANTHERPTHR24067UBIQUITIN-CONJUGATING ENZYME E2coord: 3..44
score: 1.3E-91coord: 61..132
score: 1.3
NoneNo IPR availablePANTHERPTHR24067:SF145SUBFAMILY NOT NAMEDcoord: 61..132
score: 1.3E-91coord: 3..44
score: 1.3
NoneNo IPR availableSMARTSM00212ubc_7coord: 1..129
score: 5.5

The following gene(s) are paralogous to this gene:

None