Lsi08G006420 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi08G006420
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	F1F0-ATPase inhibitor protein
Location	chr08 : 14755866 .. 14756624 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATGCGATCGGTGCTCAGTCGTTCACCGTTGACTCCCTCGACGGCGCGTATCGCTGGCGCCGGTGGATTCCGATTCCTCTCCGACGGCAAAGGTCGAGTTCTTAGTGAGGAGGAGCGTGCTGCCGAGAACGTCTACATCCAGGTTGAATTATTAGTTTCAAATGTTCGAGAAAAATGTGTAGAAACTAGTAGGACTCCGATTATCGACCTGTTTTTTTTGTAATGTTCACTTGTTAGAAACTCAGGTAGAAATGGACTGAGCGAAGTTACTGTTTCGATACTTTCTTTTTGTGAGCGAAGTTTGCGTTCTACGATTTATGATAGTTTTAGTATGATCATCAATCTGTTTGATCGAATGGTTGCTTGTTCGAAACTCAAATAAAAATAGACTGGAGTGGGAGCATATTTACTGTTTATATAAGATGATATTTTCTTTTTGTGCGAGGAGTTTGTGTTCTCCGATCCTCTACTGTGGTAGGAGTTGGCTTATCGAATTTCCACTTGTTCGAAACTTTAATAGAAATGCACAGGACTGTAAATGAAGTTATGAACTATGTGTTCCCCGATTTTTAGTTATTGTTTTTTCTAATATTTGTAGAAAATGGAAAGAGAAAAGCTGGAGAAGCAGAGGCTTAAGGCAGAGAAAGAAAAGGCCGAGAAGGAGAAAGAACAGTCTGAGAAGGTATTCATCACTGTTATAATTCTGGTTGAAATTGGCAACTATTATTTATTAGTTGATTTGTGGCATGTGTGA

mRNA sequence

ATGGCCATGCGATCGGTGCTCAGTCGTTCACCGTTGACTCCCTCGACGGCGCGTATCGCTGGCGCCGGTGGATTCCGATTCCTCTCCGACGGCAAAGGTCGAGTTCTTAGTGAGGAGGAGCGTGCTGCCGAGAACGTCTACATCCAGAAAATGGAAAGAGAAAAGCTGGAGAAGCAGAGGCTTAAGGCAGAGAAAGAAAAGGCCGAGAAGGAGAAAGAACAGTCTGAGAAGGTATTCATCACTGTTATAATTCTGGTTGAAATTGGCAACTATTATTTATTAGTTGATTTGTGGCATGTGTGA

Coding sequence (CDS)

ATGGCCATGCGATCGGTGCTCAGTCGTTCACCGTTGACTCCCTCGACGGCGCGTATCGCTGGCGCCGGTGGATTCCGATTCCTCTCCGACGGCAAAGGTCGAGTTCTTAGTGAGGAGGAGCGTGCTGCCGAGAACGTCTACATCCAGAAAATGGAAAGAGAAAAGCTGGAGAAGCAGAGGCTTAAGGCAGAGAAAGAAAAGGCCGAGAAGGAGAAAGAACAGTCTGAGAAGGTATTCATCACTGTTATAATTCTGGTTGAAATTGGCAACTATTATTTATTAGTTGATTTGTGGCATGTGTGA

Protein sequence

MAMRSVLSRSPLTPSTARIAGAGGFRFLSDGKGRVLSEEERAAENVYIQKMEREKLEKQRLKAEKEKAEKEKEQSEKVFITVIILVEIGNYYLLVDLWHV

Homology

[Close]

BLAST of Lsi08G006420 vs. TrEMBL
Match: A0A0A0K8J7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G046230 PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 8.1e-23
Identity = 68/77 (88.31%), Postives = 71/77 (92.21%), Query Frame = 1


Query: 1  MAMRSVLSRSPLTPSTARIAGAGGFRFLSDGKGRVLSEEERAAENVYIQKMEREKLEKQR 60
          MAMRSVL+RS LT STAR   AGGFRFLSDGKGRVLSEEERAAENVYIQKMERE+LEK R
Sbjct: 1  MAMRSVLTRSSLTTSTAR---AGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60

Query: 61 LKAEKEKAEKEKEQSEK 78
          LKAEKEKA+KEKEQSEK
Sbjct: 61 LKAEKEKAQKEKEQSEK 74

[Close]

BLAST of Lsi08G006420 vs. TrEMBL
Match: V4TF21_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10017346mg PE=4 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 6.8e-14
Identity = 54/77 (70.13%), Postives = 59/77 (76.62%), Query Frame = 1


Query: 1  MAMRSVLSRSPLTPSTARIAGAGGFRFLSDGKGRVLSEEERAAENVYIQKMEREKLEKQR 60
          MAMRS L R  +T S     GA   R+ SD KGRVLSEEERAAENVYIQKMERE+LEKQR
Sbjct: 1  MAMRSALCRLKVTRSMESTRGAT--RYFSDDKGRVLSEEERAAENVYIQKMERERLEKQR 60

Query: 61 LKAEKEKAEKEKEQSEK 78
           KAEKEK +KEKE +EK
Sbjct: 61 KKAEKEKLQKEKEGAEK 75

[Close]

BLAST of Lsi08G006420 vs. TrEMBL
Match: A0A067E0M2_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g034789mg PE=4 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 6.8e-14
Identity = 54/77 (70.13%), Postives = 59/77 (76.62%), Query Frame = 1


Query: 1  MAMRSVLSRSPLTPSTARIAGAGGFRFLSDGKGRVLSEEERAAENVYIQKMEREKLEKQR 60
          MAMRS L R  +T S     GA   R+ SD KGRVLSEEERAAENVYIQKMERE+LEKQR
Sbjct: 1  MAMRSALCRLKVTRSMESTRGAT--RYFSDDKGRVLSEEERAAENVYIQKMERERLEKQR 60

Query: 61 LKAEKEKAEKEKEQSEK 78
           KAEKEK +KEKE +EK
Sbjct: 61 KKAEKEKLQKEKEGAEK 75

[Close]

BLAST of Lsi08G006420 vs. TrEMBL
Match: B9HVV9_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s24670g PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 8.9e-14
Identity = 51/77 (66.23%), Postives = 59/77 (76.62%), Query Frame = 1


Query: 1  MAMRSVLSRSPLTPSTARIAGAGGFRFLSDGKGRVLSEEERAAENVYIQKMEREKLEKQR 60
          MA RS LSR  L    +  +  G  R+ SDGKGRVLSEEERAAENVYIQKMERE+LEK +
Sbjct: 1  MATRSALSRLSLITIRSMKSTRGATRYFSDGKGRVLSEEERAAENVYIQKMERERLEKLK 60

Query: 61 LKAEKEKAEKEKEQSEK 78
          LK EKEKAEKEK+ ++K
Sbjct: 61 LKQEKEKAEKEKQGADK 77

[Close]

BLAST of Lsi08G006420 vs. TrEMBL
Match: W9QGS0_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_018375 PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 3.4e-13
Identity = 54/79 (68.35%), Postives = 62/79 (78.48%), Query Frame = 1


Query: 1  MAMRSVLSR-SPLTPSTARIAGAGGFRFLSDGKGRVLSEEERAAENVYIQKMEREKLEKQ 60
          MAMRSV+ R   LT S     GA   R+LSDGKGR+LSEEERAAENVYIQKMERE+ EK 
Sbjct: 1  MAMRSVVRRVGILTRSMETTRGAT--RYLSDGKGRILSEEERAAENVYIQKMERERSEKL 60

Query: 61 RLKAEKEKAEKEKEQSEKV 79
          + KAEKEKA+KEKE ++KV
Sbjct: 61 KQKAEKEKAQKEKENADKV 77

[Close]

BLAST of Lsi08G006420 vs. TAIR10
Match: AT5G04750.1 (AT5G04750.1 F1F0-ATPase inhibitor protein, putative)

HSP 1 Score: 53.1 bits (126), Expect = 1.1e-07
Identity = 32/52 (61.54%), Postives = 42/52 (80.77%), Query Frame = 1


Query: 26 RFLSDGKGRVLSEEERAAENVYIQKMEREKLEKQRLKAEKEKAEKEKEQSEK 78
          R+ SD KGRVLSEEERA E++YIQKMERE+LEK++ K E++K + EK  + K
Sbjct: 37 RYFSDDKGRVLSEEERAKESMYIQKMERERLEKKK-KLEQDKLDGEKGSANK 87

[Close]

BLAST of Lsi08G006420 vs. NCBI nr
Match: gi|659113500|ref|XP_008456607.1| (PREDICTED: uncharacterized protein LOC103496516 [Cucumis melo])

HSP 1 Score: 126.3 bits (316), Expect = 2.9e-26
Identity = 72/77 (93.51%), Postives = 73/77 (94.81%), Query Frame = 1


Query: 1  MAMRSVLSRSPLTPSTARIAGAGGFRFLSDGKGRVLSEEERAAENVYIQKMEREKLEKQR 60
          MAMRSVLSRS LTP TAR AGAGGFRFLSDGKGRVLSEEERAAENVYIQKMERE+LEK R
Sbjct: 1  MAMRSVLSRSSLTPCTARAAGAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60

Query: 61 LKAEKEKAEKEKEQSEK 78
          LKAEKEKAEKEKEQSEK
Sbjct: 61 LKAEKEKAEKEKEQSEK 77

[Close]

BLAST of Lsi08G006420 vs. NCBI nr
Match: gi|449446299|ref|XP_004140909.1| (PREDICTED: uncharacterized protein At2g27730, mitochondrial [Cucumis sativus])

HSP 1 Score: 114.4 bits (285), Expect = 1.2e-22
Identity = 68/77 (88.31%), Postives = 71/77 (92.21%), Query Frame = 1


Query: 1  MAMRSVLSRSPLTPSTARIAGAGGFRFLSDGKGRVLSEEERAAENVYIQKMEREKLEKQR 60
          MAMRSVL+RS LT STAR   AGGFRFLSDGKGRVLSEEERAAENVYIQKMERE+LEK R
Sbjct: 1  MAMRSVLTRSSLTTSTAR---AGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60

Query: 61 LKAEKEKAEKEKEQSEK 78
          LKAEKEKA+KEKEQSEK
Sbjct: 61 LKAEKEKAQKEKEQSEK 74

[Close]

BLAST of Lsi08G006420 vs. NCBI nr
Match: gi|1009165502|ref|XP_015901073.1| (PREDICTED: uncharacterized protein At2g27730, mitochondrial-like [Ziziphus jujuba])

HSP 1 Score: 86.7 bits (213), Expect = 2.6e-14
Identity = 57/78 (73.08%), Postives = 63/78 (80.77%), Query Frame = 1


Query: 1  MAMRS-VLSRSPLTPSTARIAGAGGFRFLSDGKGRVLSEEERAAENVYIQKMEREKLEKQ 60
          MAMRS VLSR+ LT   A  +  G  R+ SD KGRVLSEEERAAEN+YIQKMERE+LEKQ
Sbjct: 1  MAMRSLVLSRAVLT--RAMESTRGPTRYFSDSKGRVLSEEERAAENIYIQKMERERLEKQ 60

Query: 61 RLKAEKEKAEKEKEQSEK 78
          +LKAEKEKAEKEKE S K
Sbjct: 61 KLKAEKEKAEKEKENSGK 76

[Close]

BLAST of Lsi08G006420 vs. NCBI nr
Match: gi|1009119695|ref|XP_015876519.1| (PREDICTED: uncharacterized protein At2g27730, mitochondrial-like [Ziziphus jujuba])

HSP 1 Score: 85.9 bits (211), Expect = 4.4e-14
Identity = 56/78 (71.79%), Postives = 62/78 (79.49%), Query Frame = 1


Query: 1  MAMRS-VLSRSPLTPSTARIAGAGGFRFLSDGKGRVLSEEERAAENVYIQKMEREKLEKQ 60
          MAMRS VLSR+ LT +     G    R+ SD KGRVLSEEERAAEN+YIQKMERE+LEKQ
Sbjct: 1  MAMRSLVLSRAVLTRTMESTRGPT--RYFSDSKGRVLSEEERAAENIYIQKMERERLEKQ 60

Query: 61 RLKAEKEKAEKEKEQSEK 78
          +LKAEKEKAEKEKE S K
Sbjct: 61 KLKAEKEKAEKEKENSGK 76

[Close]

BLAST of Lsi08G006420 vs. NCBI nr
Match: gi|567906869|ref|XP_006445748.1| (hypothetical protein CICLE_v10017346mg [Citrus clementina])

HSP 1 Score: 84.7 bits (208), Expect = 9.8e-14
Identity = 54/77 (70.13%), Postives = 59/77 (76.62%), Query Frame = 1


Query: 1  MAMRSVLSRSPLTPSTARIAGAGGFRFLSDGKGRVLSEEERAAENVYIQKMEREKLEKQR 60
          MAMRS L R  +T S     GA   R+ SD KGRVLSEEERAAENVYIQKMERE+LEKQR
Sbjct: 1  MAMRSALCRLKVTRSMESTRGAT--RYFSDDKGRVLSEEERAAENVYIQKMERERLEKQR 60

Query: 61 LKAEKEKAEKEKEQSEK 78
           KAEKEK +KEKE +EK
Sbjct: 61 KKAEKEKLQKEKEGAEK 75

The following BLAST results are available for this feature:

BLAST of Lsi08G006420 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Lsi08G006420 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K8J7_CUCSA	8.1e-23	88.31	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G046230 PE=4 SV=1	[more]
V4TF21_9ROSI	6.8e-14	70.13	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10017346mg PE=4 SV=1	[more]
A0A067E0M2_CITSI	6.8e-14	70.13	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g034789mg PE=4 SV=1	[more]
B9HVV9_POPTR	8.9e-14	66.23	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s24670g PE=4 SV=1	[more]
W9QGS0_9ROSA	3.4e-13	68.35	Uncharacterized protein OS=Morus notabilis GN=L484_018375 PE=4 SV=1	[more]

BLAST of Lsi08G006420 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G04750.1	1.1e-07	61.54	F1F0-ATPase inhibitor protein, putative	[more]

BLAST of Lsi08G006420 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659113500\|ref\|XP_008456607.1\|	2.9e-26	93.51	PREDICTED: uncharacterized protein LOC103496516 [Cucumis melo]	[more]
gi\|449446299\|ref\|XP_004140909.1\|	1.2e-22	88.31	PREDICTED: uncharacterized protein At2g27730, mitochondrial [Cucumis sativus]	[more]
gi\|1009165502\|ref\|XP_015901073.1\|	2.6e-14	73.08	PREDICTED: uncharacterized protein At2g27730, mitochondrial-like [Ziziphus jujub...	[more]
gi\|1009119695\|ref\|XP_015876519.1\|	4.4e-14	71.79	PREDICTED: uncharacterized protein At2g27730, mitochondrial-like [Ziziphus jujub...	[more]
gi\|567906869\|ref\|XP_006445748.1\|	9.8e-14	70.13	hypothetical protein CICLE_v10017346mg [Citrus clementina]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032780	negative regulation of ATPase activity
biological_process	GO:0045980	negative regulation of nucleotide metabolic process
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0008150	biological_process
cellular_component	GO:0016020	membrane
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0042030	ATPase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G006420.1	Lsi08G006420.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	unknown	Coil	Coil	coord: 44..78 scor
None	No IPR available	PANTHER	PTHR33878	FAMILY NOT NAMED	coord: 2..76 score: 3.8
None	No IPR available	PANTHER	PTHR33878:SF2	SUBFAMILY NOT NAMED	coord: 2..76 score: 3.8

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi08G006420	CmaCh15G005260	Cucurbita maxima (Rimu)	cmalsiB294
Lsi08G006420	CmaCh04G025130	Cucurbita maxima (Rimu)	cmalsiB703
Lsi08G006420	CmoCh04G026310	Cucurbita moschata (Rifu)	cmolsiB697
Lsi08G006420	CmoCh15G005330	Cucurbita moschata (Rifu)	cmolsiB282
Lsi08G006420	Cla013688	Watermelon (97103) v1	lsiwmB475
Lsi08G006420	MELO3C019903	Melon (DHL92) v3.5.1	lsimeB470
Lsi08G006420	ClCG08G008330	Watermelon (Charleston Gray)	lsiwcgB443
Lsi08G006420	MELO3C019903.2	Melon (DHL92) v3.6.1	lsimedB491
Lsi08G006420	Cla97C08G151080	Watermelon (97103) v2	lsiwmbB448
Lsi08G006420	Bhi04G001317	Wax gourd	lsiwgoB593
Lsi08G006420	Cp4.1LG01g24970	Cucurbita pepo (Zucchini)	cpelsiB382
Lsi08G006420	CsGy6G003990	Cucumber (Gy14) v2	cgyblsiB444
Lsi08G006420	Carg15855	Silver-seed gourd	carlsiB059
Lsi08G006420	Carg01673	Silver-seed gourd	carlsiB758
Lsi08G006420	CsaV3_6G004310	Cucumber (Chinese Long) v3	cuclsiB509

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi08G006420	Bottle gourd (USVL1VR-Ls)	lsilsiB152
Lsi08G006420	Cucumber (Gy14) v1	cgylsiB588
Lsi08G006420	Cucurbita maxima (Rimu)	cmalsiB706
Lsi08G006420	Cucurbita moschata (Rifu)	cmolsiB700
Lsi08G006420	Cucurbita pepo (Zucchini)	cpelsiB158
Lsi08G006420	Cucurbita pepo (Zucchini)	cpelsiB384
Lsi08G006420	Wild cucumber (PI 183967)	cpilsiB487
Lsi08G006420	Cucumber (Chinese Long) v2	culsiB389
Lsi08G006420	Cucumber (Chinese Long) v2	culsiB478
Lsi08G006420	Melon (DHL92) v3.5.1	lsimeB463
Lsi08G006420	Watermelon (Charleston Gray)	lsiwcgB435
Lsi08G006420	Watermelon (97103) v1	lsiwmB485
Lsi08G006420	Cucumber (Gy14) v2	cgyblsiB354
Lsi08G006420	Silver-seed gourd	carlsiB769
Lsi08G006420	Watermelon (97103) v2	lsiwmbB439
Lsi08G006420	Wax gourd	lsiwgoB618