Lsi07G008540 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi07G008540
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionGibberellin 2-oxidase
Locationchr07 : 9609792 .. 9611674 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAGAGAGAGAGAGAAAAAATTTTAAGCTCAAAAAGCTCTCTTTTTTCCAAAAATCTCTCACACCCTTTTGTTTGTTCTTCCTCTGTTTCTTTAAAGAAGTAAAGAGAAAAAGGGAAACTTTCATTCTCCTTCTTTTTCTCTCAAAACTTTCCATACCCAAATCCAATGGTGGTTTTGTCCAAACCATCAATCCAACAGTTGTCTTCTAATTCCTTCATGAGAAATTCCATGTCATCAATGGCTGCTGCTGCTGCTTCTTCCTCTGCAGCATTTTTCTCTGAGGTTCCATTGATAGACCTTTCTGCACCAGATGCTAAACAACTCATTGTTAAAGCTTGTGAAGAGCTTGGATTCTTCAAGGTCGTCAACCATGGCGTACCCATGGAATTCATCTCCACTCTAGAATCAGAATCCACTAATTTCTTCTCCCTTCCTCTCTCTGAAAAACAAAAAGCTGCTCCTCCTTCCCCTTTTGGCTATGGCAACAAGCAAATTGGCCGCAATGGCGATGTCGGTTGGGTCGAATATCTTCTCTTAAACACTCATCTAGAATCCAACTCCGATGGGTTCCTCTCCATTTTTGGCCAAGACCCTCAAAAACTCCGGTAACACAAAAACACCCAAATGCTCTGTTTTTTCTTCACTCTGTTTCTTGTCTTTCCTCTGTTTTTTTATGCTTCTGATTTCTGTTCTCTGTTTCTTTGTTGTAGCTCTGCTGTGAACAATTACATCTCGGCTGTGAGGAACATGGCATGTGAAATCCTGGAGTTAATGGCGGAAGGGTTGAAGATTCAGCAGAGGAATGTGTTCAGTAAACTGGTGATGGATGAAGAAAGTGACTCTGTTTTCAGGGTGAACCATTATCCGCCATGTCCAGAGCTTCAAGCTTTGAAAGGAAGGAACATGATTGGATTTGGCGAACACACAGACCCACAGATCATATCGGTTTTGAGATCAAATAATACTTCTGGACTTCAAATTTCTCTTGCTGATGGTAATTGGATTTCTGTTCCTCCTGATCAGAATTCTTTTTTCATCAATGTTGGTGACTCTTTACAGGTACAATAAAAACACATGACAGTTTCCATTCCAACCCAAAATAATAAAAATAAAAAATATGTGTTTCTCTCTCTTAATTTGTCAATCTTTGTACTATTTTTTTTTCTTTAATCTCTATAGACAAGACATGATCTTTAATAATCATGATTATGTCTGTCAGCTGGCTTTCTGTTTAGATAAATCTCCCAAGCCTCTCATGTTGGTTCTTTTTGTTTTTTGATAATCAAAAGAATTAATTTTTATTTTTTTTTATTGATATATATACTAAAATAAAAATATTTGTTTGATGAAGGTAATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGGGTTTTGACAAACAGTTTGAAGTCAAGAATTTCAATGATATATTTTGGTGGGCCACCTTTAAGTGAAAAGATAGCTCCTTTGCCTTCCCTTATGAAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACTTGGTTTGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCAGATAACAGGCTTGTGCACTTTGAAAGAATTGCAGCCTCATAATCTTCATCAAATCTCTCCTTTTAACCCCTTCATTTCTTAATATTTTCATATATAGTTTCCTTCATTAGCTACTTAGATCCCAAAATTAGGTTTTAGGGCTAACCGGTTCGCGAACATTTCATCTCTTACTCGATGATATATGTCTTCATCGGTTAGCGTTTGGATAGTTTGGTTTTGTTTGTTTTCCTTCTCATCCTTTCTTGTTTACCATAGTTTCTCTTTCCTTGTAATTACACCTATCTTTTCTTTATAATACAAAGTCTACCATTGTTTTTATAA

mRNA sequence

TGAGAGAGAGAGAGAAAAAATTTTAAGCTCAAAAAGCTCTCTTTTTTCCAAAAATCTCTCACACCCTTTTGTTTGTTCTTCCTCTGTTTCTTTAAAGAAGTAAAGAGAAAAAGGGAAACTTTCATTCTCCTTCTTTTTCTCTCAAAACTTTCCATACCCAAATCCAATGGTGGTTTTGTCCAAACCATCAATCCAACAGTTGTCTTCTAATTCCTTCATGAGAAATTCCATGTCATCAATGGCTGCTGCTGCTGCTTCTTCCTCTGCAGCATTTTTCTCTGAGGTTCCATTGATAGACCTTTCTGCACCAGATGCTAAACAACTCATTGTTAAAGCTTGTGAAGAGCTTGGATTCTTCAAGGTCGTCAACCATGGCGTACCCATGGAATTCATCTCCACTCTAGAATCAGAATCCACTAATTTCTTCTCCCTTCCTCTCTCTGAAAAACAAAAAGCTGCTCCTCCTTCCCCTTTTGGCTATGGCAACAAGCAAATTGGCCGCAATGGCGATGTCGGTTGGGTCGAATATCTTCTCTTAAACACTCATCTAGAATCCAACTCCGATGGGTTCCTCTCCATTTTTGGCCAAGACCCTCAAAAACTCCGCTCTGCTGTGAACAATTACATCTCGGCTGTGAGGAACATGGCATGTGAAATCCTGGAGTTAATGGCGGAAGGGTTGAAGATTCAGCAGAGGAATGTGTTCAGTAAACTGGTGATGGATGAAGAAAGTGACTCTGTTTTCAGGGTGAACCATTATCCGCCATGTCCAGAGCTTCAAGCTTTGAAAGGAAGGAACATGATTGGATTTGGCGAACACACAGACCCACAGATCATATCGGTTTTGAGATCAAATAATACTTCTGGACTTCAAATTTCTCTTGCTGATGGTAATTGGATTTCTGTTCCTCCTGATCAGAATTCTTTTTTCATCAATGTTGGTGACTCTTTACAGGTACAATAAAAACACATGACAGTTTCCATTCCAACCCAAAATAATAAAAATAAAAAATATGTGTTTCTCTCTCTTAATTTGTCAATCTTTGTACTATTTTTTTTTCTTTAATCTCTATAGACAAGACATGATCTTTAATAATCATGATTATGTCTGTCAGCTGGCTTTCTGTTTAGATAAATCTCCCAAGCCTCTCATGTTGGTTCTTTTTGTTTTTTGATAATCAAAAGAATTAATTTTTATTTTTTTTTATTGATATATATACTAAAATAAAAATATTTGTTTGATGAAGGTAATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGGGTTTTGACAAACAGTTTGAAGTCAAGAATTTCAATGATATATTTTGGTGGGCCACCTTTAAGTGAAAAGATAGCTCCTTTGCCTTCCCTTATGAAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACTTGGTTTGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCAGATAACAGGCTTGTGCACTTTGAAAGAATTGCAGCCTCATAATCTTCATCAAATCTCTCCTTTTAACCCCTTCATTTCTTAATATTTTCATATATAGTTTCCTTCATTAGCTACTTAGATCCCAAAATTAGGTTTTAGGGCTAACCGGTTCGCGAACATTTCATCTCTTACTCGATGATATATGTCTTCATCGGTTAGCGTTTGGATAGTTTGGTTTTGTTTGTTTTCCTTCTCATCCTTTCTTGTTTACCATAGTTTCTCTTTCCTTGTAATTACACCTATCTTTTCTTTATAATACAAAGTCTACCATTGTTTTTATAA

Coding sequence (CDS)

ATGGTGGTTTTGTCCAAACCATCAATCCAACAGTTGTCTTCTAATTCCTTCATGAGAAATTCCATGTCATCAATGGCTGCTGCTGCTGCTTCTTCCTCTGCAGCATTTTTCTCTGAGGTTCCATTGATAGACCTTTCTGCACCAGATGCTAAACAACTCATTGTTAAAGCTTGTGAAGAGCTTGGATTCTTCAAGGTCGTCAACCATGGCGTACCCATGGAATTCATCTCCACTCTAGAATCAGAATCCACTAATTTCTTCTCCCTTCCTCTCTCTGAAAAACAAAAAGCTGCTCCTCCTTCCCCTTTTGGCTATGGCAACAAGCAAATTGGCCGCAATGGCGATGTCGGTTGGGTCGAATATCTTCTCTTAAACACTCATCTAGAATCCAACTCCGATGGGTTCCTCTCCATTTTTGGCCAAGACCCTCAAAAACTCCGCTCTGCTGTGAACAATTACATCTCGGCTGTGAGGAACATGGCATGTGAAATCCTGGAGTTAATGGCGGAAGGGTTGAAGATTCAGCAGAGGAATGTGTTCAGTAAACTGGTGATGGATGAAGAAAGTGACTCTGTTTTCAGGGTGAACCATTATCCGCCATGTCCAGAGCTTCAAGCTTTGAAAGGAAGGAACATGATTGGATTTGGCGAACACACAGACCCACAGATCATATCGGTTTTGAGATCAAATAATACTTCTGGACTTCAAATTTCTCTTGCTGATGGTAATTGGATTTCTGTTCCTCCTGATCAGAATTCTTTTTTCATCAATGTTGGTGACTCTTTACAGGTACAATAA

Protein sequence

MVVLSKPSIQQLSSNSFMRNSMSSMAAAAASSSAAFFSEVPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQALKGRNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVQ
Homology
[Close]
BLAST of Lsi07G008540 vs. Swiss-Prot
Match: G2OX1_PEA (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.3e-92
Identity = 170/264 (64.39%), Postives = 207/264 (78.41%), Query Frame = 1 

Query: 1   MVVLSKPSIQQLSSNSFMRNSMSSMAAAAASSSAAFFSEVPLIDLSAPDAKQLIVKACEE 60
           MV+LSKP+ +Q +   ++RN+M             F S +PL+DLS PDAK LIVKACE+
Sbjct: 1   MVLLSKPTSEQYT---YVRNNMP----------ITFSSSIPLVDLSKPDAKTLIVKACED 60

Query: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120
            GFFKV+NHG+P++ IS LESE+  FFSLP +EK+KA P +PFGYGNK+IG NGD+GW+E
Sbjct: 61  FGFFKVINHGIPLDAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIE 120

Query: 121 YLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180
           YLLL T    N D   S++G+D  K R  + +Y  A+RNMACEIL+LMAEGLKIQ +NVF
Sbjct: 121 YLLLTT----NQDHNFSLYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVF 180

Query: 181 SKLVMDEESDSVFRVNHYPPCPELQALKGRNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240
           SKLVMD++SD +FRVNHYP CPEL A+ G N+IGFGEHTDPQIIS+LRSNNTSG QISL 
Sbjct: 181 SKLVMDKQSDCLFRVNHYPACPEL-AINGENLIGFGEHTDPQIISILRSNNTSGFQISLR 240

Query: 241 DGNWISVPPDQNSFFINVGDSLQV 265
           DG+WISVPPD +SFFINVGDSLQV
Sbjct: 241 DGSWISVPPDHSSFFINVGDSLQV 246

[Close]
BLAST of Lsi07G008540 vs. Swiss-Prot
Match: G2OX_PHACN (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.5e-85
Identity = 158/264 (59.85%), Postives = 196/264 (74.24%), Query Frame = 1 

Query: 1   MVVLSKPSIQQLSSNSFMRNSMSSMAAAAASSSAAFFSEVPLIDLSAPDAKQLIVKACEE 60
           MVVLS+P++ Q     F+     S            F+ +P++DL+ PDAK LIV AC +
Sbjct: 1   MVVLSQPALNQF----FLLKPFKS---------TPLFTGIPVVDLTHPDAKNLIVNACRD 60

Query: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120
            GFFK+VNHGVP+E ++ LE+E+  FF    SEK +A PP PFGYG+K+IG NGDVGWVE
Sbjct: 61  FGFFKLVNHGVPLELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVE 120

Query: 121 YLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180
           YLLLNT+ +  S   L IF ++P   R+ V NYI+AV+NM   +LELMAEGL I+QRN  
Sbjct: 121 YLLLNTNPDVISPKSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTL 180

Query: 181 SKLVMDEESDSVFRVNHYPPCPELQALKGRNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240
           S+L+ DE+SDS FR+NHYPPCPE+QAL  RN++GFGEHTDPQIISVLRSN+TSGLQI L 
Sbjct: 181 SRLLKDEKSDSCFRLNHYPPCPEVQAL-NRNLVGFGEHTDPQIISVLRSNSTSGLQICLT 240

Query: 241 DGNWISVPPDQNSFFINVGDSLQV 265
           DG W+SVPPDQ SFFINVGD+LQV
Sbjct: 241 DGTWVSVPPDQTSFFINVGDALQV 250

[Close]
BLAST of Lsi07G008540 vs. Swiss-Prot
Match: G2OX1_ARATH (Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA2OX1 PE=2 SV=2)

HSP 1 Score: 282.3 bits (721), Expect = 5.3e-75
Identity = 136/230 (59.13%), Postives = 171/230 (74.35%), Query Frame = 1 

Query: 37  FSEVPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQK 96
           FS +P+ID+S P++K  +VKACE+ GFFKV+NHGV  E +S LE E+ +FFSLP SEK +
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 97  AAPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISA 156
            A   PFGYGN +IGRNGDVGWVEYLL+N + +S S        + P   R+A+  Y ++
Sbjct: 75  VAG-YPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTS 134

Query: 157 VRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCP--ELQALKGRNMIG 216
           VR M  ++LE + +GL I+ RN  SKLV D+ +DS+ R+NHYPPCP    +   G+N+IG
Sbjct: 135 VRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVIG 194

Query: 217 FGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV 265
           FGEHTDPQIISVLRSNNTSGLQI+L DG+WISVPPD  SFF NVGDSLQV
Sbjct: 195 FGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQV 243

[Close]
BLAST of Lsi07G008540 vs. Swiss-Prot
Match: G2OX2_ARATH (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.1e-69
Identity = 124/225 (55.11%), Postives = 167/225 (74.22%), Query Frame = 1 

Query: 40  VPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAP 99
           +P+++L+ P+AK  IVKACEE GFFKVVNHGV  E ++ LE E+  FF LP S K +A P
Sbjct: 31  IPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGP 90

Query: 100 PSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRN 159
           P P+GYGNK+IG NGDVGW+EYLLLN + + +S    ++F Q PQ  R +V  Y+  ++ 
Sbjct: 91  PEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEIKE 150

Query: 160 MACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQALKGRNMIGFGEHT 219
           ++ ++LE++AE L I+ R+  SK++ DE+SDS  R+NHYP   E    +    +GFGEHT
Sbjct: 151 VSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEE--EAEKMVKVGFGEHT 210

Query: 220 DPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV 265
           DPQIISVLRSNNT+GLQI + DG+W++VPPD +SFFINVGD+LQV
Sbjct: 211 DPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQV 253

[Close]
BLAST of Lsi07G008540 vs. Swiss-Prot
Match: G2OX3_ARATH (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 9.4e-64
Identity = 120/225 (53.33%), Postives = 156/225 (69.33%), Query Frame = 1 

Query: 40  VPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAP 99
           +P+IDL+  DAK  IVKACEE GFFKV+NHGV  + ++ LE E+ NFF+L  S K KA P
Sbjct: 27  IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGP 86

Query: 100 PSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRN 159
           P PFGYG K+IG NGD+GW+EY+LLN +L   S    +IF   P   R AV  YI  ++ 
Sbjct: 87  PDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKR 146

Query: 160 MACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQALKGRNMIGFGEHT 219
           M+ + LE++ E LKI+ +   S+LV  +ESDS  R+NHYP   E +    +  IGFGEHT
Sbjct: 147 MSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYP---EKEETPVKEEIGFGEHT 206

Query: 220 DPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV 265
           DPQ+IS+LRSN+T GLQI + DG W+ V PD +SFF+ VGD+LQV
Sbjct: 207 DPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQV 248

[Close]
BLAST of Lsi07G008540 vs. TrEMBL
Match: A0A0A0KXI6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G075200 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 2.2e-136
Identity = 250/264 (94.70%), Postives = 256/264 (96.97%), Query Frame = 1 

Query: 1   MVVLSKPSIQQLSSNSFMRNSMSSMAAAAASSSAAFFSEVPLIDLSAPDAKQLIVKACEE 60
           MV+LSK SIQQLSSNSFMRNSM+S    AASSS AF SEVPLIDLS+PDAKQLIVKACEE
Sbjct: 1   MVILSKQSIQQLSSNSFMRNSMAS----AASSSPAFLSEVPLIDLSSPDAKQLIVKACEE 60

Query: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120
           LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE
Sbjct: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120

Query: 121 YLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180
           Y+LLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF
Sbjct: 121 YILLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180

Query: 181 SKLVMDEESDSVFRVNHYPPCPELQALKGRNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240
           SKLVMDEESDSVFRVNHYPPCP++QALKG NMIGFGEHTDPQIISVLRSNNTSGLQISLA
Sbjct: 181 SKLVMDEESDSVFRVNHYPPCPQIQALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240

Query: 241 DGNWISVPPDQNSFFINVGDSLQV 265
           DGNWISVPPDQNSFFINVGDSLQV
Sbjct: 241 DGNWISVPPDQNSFFINVGDSLQV 260

[Close]
BLAST of Lsi07G008540 vs. TrEMBL
Match: S0BDZ9_CUCSA (GA 2-oxidase OS=Cucumis sativus GN=GA2ox1 PE=2 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 6.4e-128
Identity = 230/238 (96.64%), Postives = 235/238 (98.74%), Query Frame = 1 

Query: 27  AAAASSSAAFFSEVPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNF 86
           A+AASSS AF SEVPLIDLS+PDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNF
Sbjct: 2   ASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNF 61

Query: 87  FSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 146
           FSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEY+LLNTHLESNSDGFLSIFGQDPQKL
Sbjct: 62  FSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQKL 121

Query: 147 RSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQA 206
           RSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCP++QA
Sbjct: 122 RSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQA 181

Query: 207 LKGRNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV 265
           LKG NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV
Sbjct: 182 LKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV 239

[Close]
BLAST of Lsi07G008540 vs. TrEMBL
Match: Q7X9N5_CUCMA (Gibberellin 2-oxidase OS=Cucurbita maxima GN=ga2ox PE=2 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.0e-120
Identity = 219/238 (92.02%), Postives = 228/238 (95.80%), Query Frame = 1 

Query: 27  AAAASSSAAFFSEVPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNF 86
           AAA+S SAAF+S +PLIDLSAPDAKQLIVKACEELGFFKVV HGVPME IS+LESEST F
Sbjct: 2   AAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKF 61

Query: 87  FSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 146
           FSLPLSEKQ+A PPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL
Sbjct: 62  FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 121

Query: 147 RSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQA 206
           RSAVN+YISAVRNMA EILELMAEGLKIQQRNVFSKLVMDE+SDSVFRVNHYPPCP+LQA
Sbjct: 122 RSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 181

Query: 207 LKGRNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV 265
           LKG NMIGFGEHTDPQIISVLRSNNTSG QISLADGNWISVPPD +SFFINVGDSLQV
Sbjct: 182 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQV 239

[Close]
BLAST of Lsi07G008540 vs. TrEMBL
Match: Q8GUE8_CUCMA (Gibberellin 2-oxidase OS=Cucurbita maxima GN=ga2ox PE=2 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 2.2e-120
Identity = 218/238 (91.60%), Postives = 228/238 (95.80%), Query Frame = 1 

Query: 27  AAAASSSAAFFSEVPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNF 86
           AAA+S SAAF+S +PLIDLSAPDAKQLIVKACEELGFFKVV HGVPME IS+LESEST F
Sbjct: 2   AAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKF 61

Query: 87  FSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 146
           FSLPLSEKQ+A PPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLS+FGQDPQKL
Sbjct: 62  FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSMFGQDPQKL 121

Query: 147 RSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQA 206
           RSAVN+YISAVRNMA EILELMAEGLKIQQRNVFSKLVMDE+SDSVFRVNHYPPCP+LQA
Sbjct: 122 RSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 181

Query: 207 LKGRNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV 265
           LKG NMIGFGEHTDPQIISVLRSNNTSG QISLADGNWISVPPD +SFFINVGDSLQV
Sbjct: 182 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQV 239

[Close]
BLAST of Lsi07G008540 vs. TrEMBL
Match: O04162_9ROSI (Dioxygenase OS=Marah macrocarpus PE=2 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 2.5e-111
Identity = 215/265 (81.13%), Postives = 224/265 (84.53%), Query Frame = 1 

Query: 1   MVVLSKPSIQQLSSNSFMRNSMSSMAAAAASSSAAFFSEVPLIDLSAPDAKQLIVKACEE 60
           MVVLSKP IQQLSS+S                     S VPLIDLSAPDAK LIVKACEE
Sbjct: 1   MVVLSKPEIQQLSSSSS--------------------SGVPLIDLSAPDAKHLIVKACEE 60

Query: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120
           LGFFKV+NHGVPMEFISTLESEST FFSLPLSEKQ+A PPSPFGYGNKQIGRNGDVGWVE
Sbjct: 61  LGFFKVINHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVE 120

Query: 121 YLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180
           YLL    L+SNS  FLSIFGQDPQKLRSA+N+YI AVRNMACEI+ELMAEGLKIQQRN  
Sbjct: 121 YLL----LQSNSHAFLSIFGQDPQKLRSALNDYIWAVRNMACEIVELMAEGLKIQQRNAL 180

Query: 181 SKLVMDEESDSVFRVNHYPPCP-ELQALKGRNMIGFGEHTDPQIISVLRSNNTSGLQISL 240
           SKL+M EESDSVFRVNHYPPCP ELQAL+G NMIGFGEHTDPQIISVLRSNNTSGLQISL
Sbjct: 181 SKLLMGEESDSVFRVNHYPPCPEELQALEGTNMIGFGEHTDPQIISVLRSNNTSGLQISL 240

Query: 241 ADGNWISVPPDQNSFFINVGDSLQV 265
            D NWISVPPDQ SFFINVGDSLQV
Sbjct: 241 PDANWISVPPDQTSFFINVGDSLQV 241

[Close]
BLAST of Lsi07G008540 vs. TAIR10
Match: AT1G78440.1 (AT1G78440.1 Arabidopsis thaliana gibberellin 2-oxidase 1)

HSP 1 Score: 282.3 bits (721), Expect = 3.0e-76
Identity = 136/230 (59.13%), Postives = 171/230 (74.35%), Query Frame = 1 

Query: 37  FSEVPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQK 96
           FS +P+ID+S P++K  +VKACE+ GFFKV+NHGV  E +S LE E+ +FFSLP SEK +
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 97  AAPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISA 156
            A   PFGYGN +IGRNGDVGWVEYLL+N + +S S        + P   R+A+  Y ++
Sbjct: 75  VAG-YPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTS 134

Query: 157 VRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCP--ELQALKGRNMIG 216
           VR M  ++LE + +GL I+ RN  SKLV D+ +DS+ R+NHYPPCP    +   G+N+IG
Sbjct: 135 VRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVIG 194

Query: 217 FGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV 265
           FGEHTDPQIISVLRSNNTSGLQI+L DG+WISVPPD  SFF NVGDSLQV
Sbjct: 195 FGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQV 243

[Close]
BLAST of Lsi07G008540 vs. TAIR10
Match: AT1G30040.1 (AT1G30040.1 gibberellin 2-oxidase)

HSP 1 Score: 264.6 bits (675), Expect = 6.5e-71
Identity = 124/225 (55.11%), Postives = 167/225 (74.22%), Query Frame = 1 

Query: 40  VPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAP 99
           +P+++L+ P+AK  IVKACEE GFFKVVNHGV  E ++ LE E+  FF LP S K +A P
Sbjct: 31  IPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGP 90

Query: 100 PSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRN 159
           P P+GYGNK+IG NGDVGW+EYLLLN + + +S    ++F Q PQ  R +V  Y+  ++ 
Sbjct: 91  PEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEIKE 150

Query: 160 MACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQALKGRNMIGFGEHT 219
           ++ ++LE++AE L I+ R+  SK++ DE+SDS  R+NHYP   E    +    +GFGEHT
Sbjct: 151 VSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEE--EAEKMVKVGFGEHT 210

Query: 220 DPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV 265
           DPQIISVLRSNNT+GLQI + DG+W++VPPD +SFFINVGD+LQV
Sbjct: 211 DPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQV 253

[Close]
BLAST of Lsi07G008540 vs. TAIR10
Match: AT2G34555.1 (AT2G34555.1 gibberellin 2-oxidase 3)

HSP 1 Score: 245.0 bits (624), Expect = 5.3e-65
Identity = 120/225 (53.33%), Postives = 156/225 (69.33%), Query Frame = 1 

Query: 40  VPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAP 99
           +P+IDL+  DAK  IVKACEE GFFKV+NHGV  + ++ LE E+ NFF+L  S K KA P
Sbjct: 27  IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGP 86

Query: 100 PSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRN 159
           P PFGYG K+IG NGD+GW+EY+LLN +L   S    +IF   P   R AV  YI  ++ 
Sbjct: 87  PDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKR 146

Query: 160 MACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQALKGRNMIGFGEHT 219
           M+ + LE++ E LKI+ +   S+LV  +ESDS  R+NHYP   E +    +  IGFGEHT
Sbjct: 147 MSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYP---EKEETPVKEEIGFGEHT 206

Query: 220 DPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV 265
           DPQ+IS+LRSN+T GLQI + DG W+ V PD +SFF+ VGD+LQV
Sbjct: 207 DPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQV 248

[Close]
BLAST of Lsi07G008540 vs. TAIR10
Match: AT1G02400.1 (AT1G02400.1 gibberellin 2-oxidase 6)

HSP 1 Score: 225.3 bits (573), Expect = 4.3e-59
Identity = 117/226 (51.77%), Postives = 148/226 (65.49%), Query Frame = 1 

Query: 41  PLIDLSAPDAKQL---IVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKA 100
           P+ID S  D  +L   IVKACE  GFFKV+NHGV  E I   E E   FF+ P S+K +A
Sbjct: 25  PVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEHEGEEFFNKPESDKLRA 84

Query: 101 APPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAV 160
            P SPFGYG K IG NGD+G +EYLLL+ +  + +D   +I   DP K  SA N+YI  V
Sbjct: 85  GPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHDDPFKFSSATNDYIRTV 144

Query: 161 RNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQALKGRNMIGFGE 220
           R++ACEI++L  E L  Q+ +  S+L+ D  SDS+ R+NHYPP P   AL G   IGFGE
Sbjct: 145 RDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPAP--YALSGVGQIGFGE 204

Query: 221 HTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQ 264
           H+DPQI++VLRSN+  GL+I   DG WI +P D   FF+ VGD LQ
Sbjct: 205 HSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVLVGDCLQ 248

[Close]
BLAST of Lsi07G008540 vs. TAIR10
Match: AT1G47990.1 (AT1G47990.1 gibberellin 2-oxidase 4)

HSP 1 Score: 206.5 bits (524), Expect = 2.1e-53
Identity = 116/236 (49.15%), Postives = 147/236 (62.29%), Query Frame = 1 

Query: 39  EVPLIDLSAPDAK--QLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQK 98
           ++P+ID+S   ++    IVKACE LGFFKV+NHGV    IS +E ES NFF+ P  EK+ 
Sbjct: 14  DIPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFFAKPAHEKKS 73

Query: 99  AAPPS-PFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYIS 158
             P + PF YG + IG NGD G VEYLL +T    N   F S          SAVN YI 
Sbjct: 74  VRPVNQPFRYGFRDIGLNGDSGEVEYLLFHT----NDPAFRS-----QLSFSSAVNCYIE 133

Query: 159 AVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPEL-------QALK 218
           AV+ +A EIL+L AEGL +   + FS+L+   +SDSV RVNHYPP  +            
Sbjct: 134 AVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFGEANLSDQSV 193

Query: 219 GRNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV 265
               +GFGEHTDPQI++VLRSN   GLQ+S +DG W+SV PD ++F +NVGD LQV
Sbjct: 194 SLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVNVGDLLQV 239

[Close]
BLAST of Lsi07G008540 vs. NCBI nr
Match: gi|659101776|ref|XP_008451787.1| (PREDICTED: gibberellin 2-beta-dioxygenase 1 [Cucumis melo])

HSP 1 Score: 496.1 bits (1276), Expect = 3.7e-137
Identity = 252/264 (95.45%), Postives = 257/264 (97.35%), Query Frame = 1 

Query: 1   MVVLSKPSIQQLSSNSFMRNSMSSMAAAAASSSAAFFSEVPLIDLSAPDAKQLIVKACEE 60
           MVVLSK SIQQLSSNSFMRNSM    A+AASSSAAFFSEVPLIDLS+PDAKQLIVKACEE
Sbjct: 1   MVVLSKQSIQQLSSNSFMRNSM----ASAASSSAAFFSEVPLIDLSSPDAKQLIVKACEE 60

Query: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120
           LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE
Sbjct: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120

Query: 121 YLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180
           Y+LLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRN F
Sbjct: 121 YILLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNAF 180

Query: 181 SKLVMDEESDSVFRVNHYPPCPELQALKGRNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240
           SKLVMDEESDSVFRVNHYPPCP++QALKG NMIGFGEHTDPQIISVLRSNNTSGLQISLA
Sbjct: 181 SKLVMDEESDSVFRVNHYPPCPQIQALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240

Query: 241 DGNWISVPPDQNSFFINVGDSLQV 265
           DGNWISVPPDQNSFFINVGDSLQV
Sbjct: 241 DGNWISVPPDQNSFFINVGDSLQV 260

[Close]
BLAST of Lsi07G008540 vs. NCBI nr
Match: gi|700198381|gb|KGN53539.1| (hypothetical protein Csa_4G075200 [Cucumis sativus])

HSP 1 Score: 493.0 bits (1268), Expect = 3.2e-136
Identity = 250/264 (94.70%), Postives = 256/264 (96.97%), Query Frame = 1 

Query: 1   MVVLSKPSIQQLSSNSFMRNSMSSMAAAAASSSAAFFSEVPLIDLSAPDAKQLIVKACEE 60
           MV+LSK SIQQLSSNSFMRNSM+S    AASSS AF SEVPLIDLS+PDAKQLIVKACEE
Sbjct: 1   MVILSKQSIQQLSSNSFMRNSMAS----AASSSPAFLSEVPLIDLSSPDAKQLIVKACEE 60

Query: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120
           LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE
Sbjct: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120

Query: 121 YLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180
           Y+LLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF
Sbjct: 121 YILLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180

Query: 181 SKLVMDEESDSVFRVNHYPPCPELQALKGRNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240
           SKLVMDEESDSVFRVNHYPPCP++QALKG NMIGFGEHTDPQIISVLRSNNTSGLQISLA
Sbjct: 181 SKLVMDEESDSVFRVNHYPPCPQIQALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240

Query: 241 DGNWISVPPDQNSFFINVGDSLQV 265
           DGNWISVPPDQNSFFINVGDSLQV
Sbjct: 241 DGNWISVPPDQNSFFINVGDSLQV 260

[Close]
BLAST of Lsi07G008540 vs. NCBI nr
Match: gi|793421968|ref|NP_001292640.1| (gibberellin 2-beta-dioxygenase 1 [Cucumis sativus])

HSP 1 Score: 464.9 bits (1195), Expect = 9.2e-128
Identity = 230/238 (96.64%), Postives = 235/238 (98.74%), Query Frame = 1 

Query: 27  AAAASSSAAFFSEVPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNF 86
           A+AASSS AF SEVPLIDLS+PDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNF
Sbjct: 2   ASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNF 61

Query: 87  FSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 146
           FSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEY+LLNTHLESNSDGFLSIFGQDPQKL
Sbjct: 62  FSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQKL 121

Query: 147 RSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQA 206
           RSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCP++QA
Sbjct: 122 RSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQA 181

Query: 207 LKGRNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV 265
           LKG NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV
Sbjct: 182 LKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV 239

[Close]
BLAST of Lsi07G008540 vs. NCBI nr
Match: gi|32127337|emb|CAC85924.1| (Gibberellin 2-oxidase [Cucurbita maxima])

HSP 1 Score: 441.0 bits (1133), Expect = 1.4e-120
Identity = 219/238 (92.02%), Postives = 228/238 (95.80%), Query Frame = 1 

Query: 27  AAAASSSAAFFSEVPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNF 86
           AAA+S SAAF+S +PLIDLSAPDAKQLIVKACEELGFFKVV HGVPME IS+LESEST F
Sbjct: 2   AAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKF 61

Query: 87  FSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 146
           FSLPLSEKQ+A PPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL
Sbjct: 62  FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 121

Query: 147 RSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQA 206
           RSAVN+YISAVRNMA EILELMAEGLKIQQRNVFSKLVMDE+SDSVFRVNHYPPCP+LQA
Sbjct: 122 RSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 181

Query: 207 LKGRNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV 265
           LKG NMIGFGEHTDPQIISVLRSNNTSG QISLADGNWISVPPD +SFFINVGDSLQV
Sbjct: 182 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQV 239

[Close]
BLAST of Lsi07G008540 vs. NCBI nr
Match: gi|27123665|emb|CAC83090.1| (gibberellin 2-oxidase [Cucurbita maxima])

HSP 1 Score: 439.9 bits (1130), Expect = 3.2e-120
Identity = 218/238 (91.60%), Postives = 228/238 (95.80%), Query Frame = 1 

Query: 27  AAAASSSAAFFSEVPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNF 86
           AAA+S SAAF+S +PLIDLSAPDAKQLIVKACEELGFFKVV HGVPME IS+LESEST F
Sbjct: 2   AAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKF 61

Query: 87  FSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 146
           FSLPLSEKQ+A PPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLS+FGQDPQKL
Sbjct: 62  FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSMFGQDPQKL 121

Query: 147 RSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQA 206
           RSAVN+YISAVRNMA EILELMAEGLKIQQRNVFSKLVMDE+SDSVFRVNHYPPCP+LQA
Sbjct: 122 RSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 181

Query: 207 LKGRNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV 265
           LKG NMIGFGEHTDPQIISVLRSNNTSG QISLADGNWISVPPD +SFFINVGDSLQV
Sbjct: 182 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQV 239

The following BLAST results are available for this feature:
BLAST of Lsi07G008540 vs. Swiss-Prot
Match NameE-valueIdentityDescription
G2OX1_PEA1.3e-9264.39Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum GN=GA2OX1 PE=1 SV=1[more]
G2OX_PHACN1.5e-8559.85Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus GN=GA2OX1 PE=2 SV=1[more]
G2OX1_ARATH5.3e-7559.13Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA2OX1 PE=2 SV=2[more]
G2OX2_ARATH1.1e-6955.11Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana GN=GA2OX2 PE=2 SV=1[more]
G2OX3_ARATH9.4e-6453.33Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana GN=GA2OX3 PE=2 SV=1[more]
BLAST of Lsi07G008540 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KXI6_CUCSA2.2e-13694.70Uncharacterized protein OS=Cucumis sativus GN=Csa_4G075200 PE=3 SV=1[more]
S0BDZ9_CUCSA6.4e-12896.64GA 2-oxidase OS=Cucumis sativus GN=GA2ox1 PE=2 SV=1[more]
Q7X9N5_CUCMA1.0e-12092.02Gibberellin 2-oxidase OS=Cucurbita maxima GN=ga2ox PE=2 SV=1[more]
Q8GUE8_CUCMA2.2e-12091.60Gibberellin 2-oxidase OS=Cucurbita maxima GN=ga2ox PE=2 SV=1[more]
O04162_9ROSI2.5e-11181.13Dioxygenase OS=Marah macrocarpus PE=2 SV=1[more]
BLAST of Lsi07G008540 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G78440.13.0e-7659.13 Arabidopsis thaliana gibberellin 2-oxidase 1[more]
AT1G30040.16.5e-7155.11 gibberellin 2-oxidase[more]
AT2G34555.15.3e-6553.33 gibberellin 2-oxidase 3[more]
AT1G02400.14.3e-5951.77 gibberellin 2-oxidase 6[more]
AT1G47990.12.1e-5349.15 gibberellin 2-oxidase 4[more]
BLAST of Lsi07G008540 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659101776|ref|XP_008451787.1|3.7e-13795.45PREDICTED: gibberellin 2-beta-dioxygenase 1 [Cucumis melo][more]
gi|700198381|gb|KGN53539.1|3.2e-13694.70hypothetical protein Csa_4G075200 [Cucumis sativus][more]
gi|793421968|ref|NP_001292640.1|9.2e-12896.64gibberellin 2-beta-dioxygenase 1 [Cucumis sativus][more]
gi|32127337|emb|CAC85924.1|1.4e-12092.02Gibberellin 2-oxidase [Cucurbita maxima][more]
gi|27123665|emb|CAC83090.1|3.2e-12091.60gibberellin 2-oxidase [Cucurbita maxima][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
GO:0005506iron ion binding
Vocabulary: INTERPRO
TermDefinition
IPR027443IPNS-like
IPR026992DIOX_N
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR002283Isopenicillin N synthase
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045487 gibberellin catabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0009416 response to light stimulus
cellular_component GO:0005575 cellular_component
molecular_function GO:0052634 C-19 gibberellin 2-beta-dioxygenase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0046872 metal ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G008540.1Lsi07G008540.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002283Isopenicillin N synthasePRINTSPR00682IPNSYNTHASEcoord: 218..239
score: 3.4E-5coord: 54..71
score: 3.
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 190..264
score: 2.4
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 188..265
score: 8
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 40..131
score: 1.2
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 22..264
score: 1.5
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 1..264
score: 2.4E
NoneNo IPR availablePANTHERPTHR10209:SF91GIBBERELLIN 2-BETA-DIOXYGENASE 1coord: 1..264
score: 2.4E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 35..264
score: 1.3