CmaCh04G006490 (gene) Cucurbita maxima (Rimu)

Overview
NameCmaCh04G006490
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionGibberellin 2-oxidase
LocationCma_Chr04 : 3321297 .. 3322998 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGTTATCACTCTCCTTCCTCCTAAAAGATCATCGCCACTCTGCGTTCTCCTCTGTTTCAAAGCTTTGTGTAAAACAAAAACCCCAGCCTCCATTCTAAAACTAGCCTTTGAAAGATTATTATTATAAAGAAAAAGACCACTTTCTCTTTCTCTCTCTACAACCCACCATTTCCATTTCTTATAAATACCCTCCCCTCCATCAGCACTGCATTGCCTCCCATTTGTTCTTCCTCTGTCTCTCTGAAGAAGAAGAGAGAAAAAGGGAAGCTTTCATTCTCCTTCTTCTTGTTTTTCACTCGAAGCTCTCAAAACCCATATCAATGGTGGTTTTGTCTAAGCCATCTATCAAACAGATGGCTTCTTCCTACATGAGAAACTCCATGGCTGCTGCTGGTTCTTCCTCTGCTGCATTTTTCTCTGGGGTTCCGTTGATTGACCTTTCTGCACCAGATGCTAAGCATCTTATCGTCAAGGCCTGTGAAGAGCTTGGATTCTTCAAGGTCGTCAACCATGGCGTTCCCATGGAATTCATCTCCACTCTTGAATCAGAATCCACCAAATTCTTCTCCCTTCCCCTCTCTGAAAAACAGAGGGCCGGCCCCCCTTCTCCTTTTGGCTATGGCAATAAACAAATTGGCCGCAATGGCGACGTGGGCTGGGTTGAATATCTCCTCTTGAATACGCACCTTGAGTCCAAATCTGATGGGTTCCTCTCCATTTTTGGCCAAGACCCACAAAAGCTCCGGTAACCAAAATCCCCGTTCTTCTAAACGCTCTGTTTTTCTACTCTGTTTCTGACCTTGTGTGCTCTGTTTCTGTGTTGTAGCTCTGCTGTGAACGATTACATCTCGGCTGTGAGAAACATGGCGTGTGAAATCCTTGAACTAATGGCGGAAGGTTTGAAGATTCAGCAGAGGAATGCGTTCAGCAAGCTGTTGATGGATGAAGAGAGCGACTCTGTTTTCAGGGTGAACCATTACCCGCCATGTCCAGAGCTTCATGCTTTGAAAGGGACAAACATGATTGGATTTGGAGAACACACAGACCCACAGATCATTTCGGTTTTGAGATCGAATAACACTTCAGGACTTCAAATATCTCTTGCTGATGGGAATTGGATTTCCGTTCCTCCCGATCAAAGCTCCTTCTTCATCAACGTAGGTGACTCTTTACAGGTACAATAAACAACACGACAATCCAAACCCAGTTTTGATTTCGACCAAAGAAGTAAAAGACAGGAACTTTTTGTTGATATAAACTAAAAAGAAGTGAACTTTTTGTTGGTGAAGGTGATGACGAATGGAAGATTCAAAAGCGTGAAGCACAGAGTATTGACGAACAGGTTGAAGTCAAGAGTTTCAATGATATACTTCGGGGGGCCGCCGTTGAGTGAAAAGATAGCGCCGTTGCCTTCGCTCATGAAAGGAGAAGAAAGTTTGTACAAAGAGTTCACTTGGTTCGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCAGATAACAGGCTTGTGCACTTTGAGAGAATTGCAGCCTCATGATCTAATCTTTACTCTTTTTTCTTTTTTATTTTCAAATATAGTCCCCTCTCTCTCTCTCCCCCTTTTAGCTTCCAAAATTAGGGTTCTTAGATGTTTGGATAGTCTTTGGGTTTGTAATTCCTCTCTCTTGTTTCCTATAGCAGACAAATGATATTTGACTTTCA

mRNA sequence

AGTTATCACTCTCCTTCCTCCTAAAAGATCATCGCCACTCTGCGTTCTCCTCTGTTTCAAAGCTTTGTGTAAAACAAAAACCCCAGCCTCCATTCTAAAACTAGCCTTTGAAAGATTATTATTATAAAGAAAAAGACCACTTTCTCTTTCTCTCTCTACAACCCACCATTTCCATTTCTTATAAATACCCTCCCCTCCATCAGCACTGCATTGCCTCCCATTTGTTCTTCCTCTGTCTCTCTGAAGAAGAAGAGAGAAAAAGGGAAGCTTTCATTCTCCTTCTTCTTGTTTTTCACTCGAAGCTCTCAAAACCCATATCAATGGTGGTTTTGTCTAAGCCATCTATCAAACAGATGGCTTCTTCCTACATGAGAAACTCCATGGCTGCTGCTGGTTCTTCCTCTGCTGCATTTTTCTCTGGGGTTCCGTTGATTGACCTTTCTGCACCAGATGCTAAGCATCTTATCGTCAAGGCCTGTGAAGAGCTTGGATTCTTCAAGGTCGTCAACCATGGCGTTCCCATGGAATTCATCTCCACTCTTGAATCAGAATCCACCAAATTCTTCTCCCTTCCCCTCTCTGAAAAACAGAGGGCCGGCCCCCCTTCTCCTTTTGGCTATGGCAATAAACAAATTGGCCGCAATGGCGACGTGGGCTGGGTTGAATATCTCCTCTTGAATACGCACCTTGAGTCCAAATCTGATGGGTTCCTCTCCATTTTTGGCCAAGACCCACAAAAGCTCCGCTCTGCTGTGAACGATTACATCTCGGCTGTGAGAAACATGGCGTGTGAAATCCTTGAACTAATGGCGGAAGGTTTGAAGATTCAGCAGAGGAATGCGTTCAGCAAGCTGTTGATGGATGAAGAGAGCGACTCTGTTTTCAGGGTGAACCATTACCCGCCATGTCCAGAGCTTCATGCTTTGAAAGGGACAAACATGATTGGATTTGGAGAACACACAGACCCACAGATCATTTCGGTTTTGAGATCGAATAACACTTCAGGACTTCAAATATCTCTTGCTGATGGGAATTGGATTTCCGTTCCTCCCGATCAAAGCTCCTTCTTCATCAACGTAGGTGACTCTTTACAGGTGATGACGAATGGAAGATTCAAAAGCGTGAAGCACAGAGTATTGACGAACAGGTTGAAGTCAAGAGTTTCAATGATATACTTCGGGGGGCCGCCGTTGAGTGAAAAGATAGCGCCGTTGCCTTCGCTCATGAAAGGAGAAGAAAGTTTGTACAAAGAGTTCACTTGGTTCGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCAGATAACAGGCTTGTGCACTTTGAGAGAATTGCAGCCTCATGATCTAATCTTTACTCTTTTTTCTTTTTTATTTTCAAATATAGTCCCCTCTCTCTCTCTCCCCCTTTTAGCTTCCAAAATTAGGGTTCTTAGATGTTTGGATAGTCTTTGGGTTTGTAATTCCTCTCTCTTGTTTCCTATAGCAGACAAATGATATTTGACTTTCA

Coding sequence (CDS)

ATGGTGGTTTTGTCTAAGCCATCTATCAAACAGATGGCTTCTTCCTACATGAGAAACTCCATGGCTGCTGCTGGTTCTTCCTCTGCTGCATTTTTCTCTGGGGTTCCGTTGATTGACCTTTCTGCACCAGATGCTAAGCATCTTATCGTCAAGGCCTGTGAAGAGCTTGGATTCTTCAAGGTCGTCAACCATGGCGTTCCCATGGAATTCATCTCCACTCTTGAATCAGAATCCACCAAATTCTTCTCCCTTCCCCTCTCTGAAAAACAGAGGGCCGGCCCCCCTTCTCCTTTTGGCTATGGCAATAAACAAATTGGCCGCAATGGCGACGTGGGCTGGGTTGAATATCTCCTCTTGAATACGCACCTTGAGTCCAAATCTGATGGGTTCCTCTCCATTTTTGGCCAAGACCCACAAAAGCTCCGCTCTGCTGTGAACGATTACATCTCGGCTGTGAGAAACATGGCGTGTGAAATCCTTGAACTAATGGCGGAAGGTTTGAAGATTCAGCAGAGGAATGCGTTCAGCAAGCTGTTGATGGATGAAGAGAGCGACTCTGTTTTCAGGGTGAACCATTACCCGCCATGTCCAGAGCTTCATGCTTTGAAAGGGACAAACATGATTGGATTTGGAGAACACACAGACCCACAGATCATTTCGGTTTTGAGATCGAATAACACTTCAGGACTTCAAATATCTCTTGCTGATGGGAATTGGATTTCCGTTCCTCCCGATCAAAGCTCCTTCTTCATCAACGTAGGTGACTCTTTACAGGTGATGACGAATGGAAGATTCAAAAGCGTGAAGCACAGAGTATTGACGAACAGGTTGAAGTCAAGAGTTTCAATGATATACTTCGGGGGGCCGCCGTTGAGTGAAAAGATAGCGCCGTTGCCTTCGCTCATGAAAGGAGAAGAAAGTTTGTACAAAGAGTTCACTTGGTTCGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCAGATAACAGGCTTGTGCACTTTGAGAGAATTGCAGCCTCATGA

Protein sequence

MVVLSKPSIKQMASSYMRNSMAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESKSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPELHALKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Homology
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BLAST of CmaCh04G006490 vs. Swiss-Prot
Match: G2OX1_PEA (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 4.2e-133
Identity = 237/340 (69.71%), Postives = 281/340 (82.65%), Query Frame = 1 

Query: 1   MVVLSKPSIKQMASSYMRNSMAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFK 60
           MV+LSKP+ +Q   +Y+RN+M    SSS      +PL+DLS PDAK LIVKACE+ GFFK
Sbjct: 1   MVLLSKPTSEQY--TYVRNNMPITFSSS------IPLVDLSKPDAKTLIVKACEDFGFFK 60

Query: 61  VVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLN 120
           V+NHG+P++ IS LESE+ KFFSLP +EK++AGP +PFGYGNK+IG NGD+GW+EYLLL 
Sbjct: 61  VINHGIPLDAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIEYLLLT 120

Query: 121 THLESKSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLM 180
           T+     D   S++G+D  K R  + DY  A+RNMACEIL+LMAEGLKIQ +N FSKL+M
Sbjct: 121 TN----QDHNFSLYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVM 180

Query: 181 DEESDSVFRVNHYPPCPELHALKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWI 240
           D++SD +FRVNHYP CPEL A+ G N+IGFGEHTDPQIIS+LRSNNTSG QISL DG+WI
Sbjct: 181 DKQSDCLFRVNHYPACPEL-AINGENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWI 240

Query: 241 SVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLPS 300
           SVPPD SSFFINVGDSLQVMTNGRFKSV+HRVL N +  R+SMIYF GPPLSEKIAPLPS
Sbjct: 241 SVPPDHSSFFINVGDSLQVMTNGRFKSVRHRVLANGIDPRLSMIYFCGPPLSEKIAPLPS 300

Query: 301 LMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 341
           LMKG+ESLYKEFTWFEYK S Y SRLADNRL ++ERIAA+
Sbjct: 301 LMKGKESLYKEFTWFEYKSSTYGSRLADNRLGNYERIAAT 327

[Close]
BLAST of CmaCh04G006490 vs. Swiss-Prot
Match: G2OX_PHACN (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 6.7e-123
Identity = 220/340 (64.71%), Postives = 266/340 (78.24%), Query Frame = 1 

Query: 1   MVVLSKPSIKQMASSYMRNSMAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFK 60
           MVVLS+P++ Q                S   F+G+P++DL+ PDAK+LIV AC + GFFK
Sbjct: 1   MVVLSQPALNQF--------FLLKPFKSTPLFTGIPVVDLTHPDAKNLIVNACRDFGFFK 60

Query: 61  VVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLN 120
           +VNHGVP+E ++ LE+E+ +FF    SEK RAGPP PFGYG+K+IG NGDVGWVEYLLLN
Sbjct: 61  LVNHGVPLELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLN 120

Query: 121 THLESKSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLM 180
           T+ +  S   L IF ++P   R+ V +YI+AV+NM   +LELMAEGL I+QRN  S+LL 
Sbjct: 121 TNPDVISPKSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLK 180

Query: 181 DEESDSVFRVNHYPPCPELHALKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWI 240
           DE+SDS FR+NHYPPCPE+ AL   N++GFGEHTDPQIISVLRSN+TSGLQI L DG W+
Sbjct: 181 DEKSDSCFRLNHYPPCPEVQAL-NRNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWV 240

Query: 241 SVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLPS 300
           SVPPDQ+SFFINVGD+LQVMTNGRFKSVKHRVL +  KSR+SMIYFGGP LSE IAPLPS
Sbjct: 241 SVPPDQTSFFINVGDALQVMTNGRFKSVKHRVLADTTKSRLSMIYFGGPALSENIAPLPS 300

Query: 301 LM-KGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAA 340
           +M KGEE LYKEFTW EYK++AY SRLADNRL  F++ AA
Sbjct: 301 VMLKGEECLYKEFTWCEYKKAAYTSRLADNRLAPFQKSAA 331

[Close]
BLAST of CmaCh04G006490 vs. Swiss-Prot
Match: G2OX1_ARATH (Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA2OX1 PE=2 SV=2)

HSP 1 Score: 386.3 bits (991), Expect = 3.4e-106
Identity = 193/309 (62.46%), Postives = 235/309 (76.05%), Query Frame = 1 

Query: 32  FSGVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISTLESESTKFFSLPLSEK-Q 91
           FS +P+ID+S P++KH +VKACE+ GFFKV+NHGV  E +S LE E+  FFSLP SEK Q
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 92  RAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESKSDGFLSIFGQDPQKLRSAVNDYIS 151
            AG   PFGYGN +IGRNGDVGWVEYLL+N + +S S        + P   R+A+ +Y +
Sbjct: 75  VAG--YPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTT 134

Query: 152 AVRNMACEILELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPELHALK--GTNMI 211
           +VR M  ++LE + +GL I+ RN  SKL+ D+ +DS+ R+NHYPPCP  +     G N+I
Sbjct: 135 SVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVI 194

Query: 212 GFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQSSFFINVGDSLQVMTNGRFKSV 271
           GFGEHTDPQIISVLRSNNTSGLQI+L DG+WISVPPD +SFF NVGDSLQVMTNGRFKSV
Sbjct: 195 GFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSV 254

Query: 272 KHRVLTNRLKSRVSMIYFGGPPLSEKIAPLPSLMKGE-ESLYKEFTWFEYKRSAYNSRLA 331
           +HRVL N  KSRVSMIYF GP L+++IAPL  L+  E E LY+EFTW EYK S YNSRL+
Sbjct: 255 RHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLS 314

Query: 332 DNRLVHFER 337
           DNRL  FER
Sbjct: 315 DNRLQQFER 321

[Close]
BLAST of CmaCh04G006490 vs. Swiss-Prot
Match: G2OX2_ARATH (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 1.7e-102
Identity = 181/303 (59.74%), Postives = 231/303 (76.24%), Query Frame = 1 

Query: 35  VPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQRAGP 94
           +P+++L+ P+AK  IVKACEE GFFKVVNHGV  E ++ LE E+  FF LP S K RAGP
Sbjct: 31  IPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGP 90

Query: 95  PSPFGYGNKQIGRNGDVGWVEYLLLNTHLESKSDGFLSIFGQDPQKLRSAVNDYISAVRN 154
           P P+GYGNK+IG NGDVGW+EYLLLN + +  S    ++F Q PQ  R +V +Y+  ++ 
Sbjct: 91  PEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEIKE 150

Query: 155 MACEILELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPELHALKGTNMIGFGEHT 214
           ++ ++LE++AE L I+ R+  SK+L DE+SDS  R+NHYP   E    +    +GFGEHT
Sbjct: 151 VSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEE--EAEKMVKVGFGEHT 210

Query: 215 DPQIISVLRSNNTSGLQISLADGNWISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLT 274
           DPQIISVLRSNNT+GLQI + DG+W++VPPD SSFFINVGD+LQVMTNGRFKSVKHRVL 
Sbjct: 211 DPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVKHRVLA 270

Query: 275 NRLKSRVSMIYFGGPPLSEKIAPLPSLMKGEES-LYKEFTWFEYKRSAYNSRLADNRLVH 334
           +  +SR+SMIYFGGPPLS+KIAPLP L+  ++  LYKEFTW +YK SAY S+L D RL  
Sbjct: 271 DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGL 330

Query: 335 FER 337
           FE+
Sbjct: 331 FEK 331

[Close]
BLAST of CmaCh04G006490 vs. Swiss-Prot
Match: G2OX3_ARATH (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 1.9e-93
Identity = 171/303 (56.44%), Postives = 219/303 (72.28%), Query Frame = 1 

Query: 35  VPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQRAGP 94
           +P+IDL+  DAK  IVKACEE GFFKV+NHGV  + ++ LE E+  FF+L  S K +AGP
Sbjct: 27  IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGP 86

Query: 95  PSPFGYGNKQIGRNGDVGWVEYLLLNTHLESKSDGFLSIFGQDPQKLRSAVNDYISAVRN 154
           P PFGYG K+IG NGD+GW+EY+LLN +L  +S    +IF   P   R AV +YI  ++ 
Sbjct: 87  PDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKR 146

Query: 155 MACEILELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPELHALKGTNMIGFGEHT 214
           M+ + LE++ E LKI+ +   S+L+  +ESDS  R+NHYP   E    +    IGFGEHT
Sbjct: 147 MSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYPEKEETPVKEE---IGFGEHT 206

Query: 215 DPQIISVLRSNNTSGLQISLADGNWISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLT 274
           DPQ+IS+LRSN+T GLQI + DG W+ V PD SSFF+ VGD+LQVMTNGRFKSVKHRV+T
Sbjct: 207 DPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVT 266

Query: 275 NRLKSRVSMIYFGGPPLSEKIAPLPSLM-KGEESLYKEFTWFEYKRSAYNSRLADNRLVH 334
           N  +SR+SMIYF GPPLSEKIAPL  L+ K ++ LY EFTW +YK SAY ++L D RL  
Sbjct: 267 NTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGL 326

Query: 335 FER 337
           FE+
Sbjct: 327 FEK 326

[Close]
BLAST of CmaCh04G006490 vs. TrEMBL
Match: A0A0A0KXI6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G075200 PE=3 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 9.0e-175
Identity = 312/342 (91.23%), Postives = 327/342 (95.61%), Query Frame = 1 

Query: 1   MVVLSKPSIKQMAS-SYMRNSMAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFF 60
           MV+LSK SI+Q++S S+MRNSMA+A SSS AF S VPLIDLS+PDAK LIVKACEELGFF
Sbjct: 1   MVILSKQSIQQLSSNSFMRNSMASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFF 60

Query: 61  KVVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLL 120
           KVVNHGVPMEFISTLESEST FFSLPLSEKQ+A PPSPFGYGNKQIGRNGDVGWVEY+LL
Sbjct: 61  KVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILL 120

Query: 121 NTHLESKSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLL 180
           NTHLES SDGFLSIFGQDPQKLRSAVN+YISAVRNMACEILELMAEGLKIQQRN FSKL+
Sbjct: 121 NTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLV 180

Query: 181 MDEESDSVFRVNHYPPCPELHALKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240
           MDEESDSVFRVNHYPPCP++ ALKG NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW
Sbjct: 181 MDEESDSVFRVNHYPPCPQIQALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240

Query: 241 ISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLP 300
           ISVPPDQ+SFFINVGDSLQVMTNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSEKIAPLP
Sbjct: 241 ISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLP 300

Query: 301 SLMKGEE-SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 341
           SLMKGEE SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 SLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 342

[Close]
BLAST of CmaCh04G006490 vs. TrEMBL
Match: S0BDZ9_CUCSA (GA 2-oxidase OS=Cucumis sativus GN=GA2ox1 PE=2 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 2.2e-168
Identity = 298/321 (92.83%), Postives = 308/321 (95.95%), Query Frame = 1 

Query: 21  MAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISTLESESTK 80
           MA+A SSS AF S VPLIDLS+PDAK LIVKACEELGFFKVVNHGVPMEFISTLESEST 
Sbjct: 1   MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60

Query: 81  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESKSDGFLSIFGQDPQK 140
           FFSLPLSEKQ+A PPSPFGYGNKQIGRNGDVGWVEY+LLNTHLES SDGFLSIFGQDPQK
Sbjct: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120

Query: 141 LRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPELH 200
           LRSAVN+YISAVRNMACEILELMAEGLKIQQRN FSKL+MDEESDSVFRVNHYPPCP++ 
Sbjct: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 180

Query: 201 ALKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQSSFFINVGDSLQVM 260
           ALKG NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQ+SFFINVGDSLQVM
Sbjct: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240

Query: 261 TNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLPSLMKGEE-SLYKEFTWFEYKR 320
           TNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSEKIAPLPSLMKGEE SLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300

Query: 321 SAYNSRLADNRLVHFERIAAS 341
           SAYNSRLADNRLVHFERIAAS
Sbjct: 301 SAYNSRLADNRLVHFERIAAS 321

[Close]
BLAST of CmaCh04G006490 vs. TrEMBL
Match: Q7X9N5_CUCMA (Gibberellin 2-oxidase OS=Cucurbita maxima GN=ga2ox PE=2 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 4.5e-166
Identity = 298/321 (92.83%), Postives = 305/321 (95.02%), Query Frame = 1 

Query: 21  MAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISTLESESTK 80
           MAAA S SAAF+SG+PLIDLSAPDAK LIVKACEELGFFKVV HGVPME IS+LESESTK
Sbjct: 1   MAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTK 60

Query: 81  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESKSDGFLSIFGQDPQK 140
           FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLES SDGFLSIFGQDPQK
Sbjct: 61  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQK 120

Query: 141 LRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPELH 200
           LRSAVNDYISAVRNMA EILELMAEGLKIQQRN FSKL+MDE+SDSVFRVNHYPPCP+L 
Sbjct: 121 LRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQ 180

Query: 201 ALKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQSSFFINVGDSLQVM 260
           ALKGTNMIGFGEHTDPQIISVLRSNNTSG QISLADGNWISVPPD SSFFINVGDSLQVM
Sbjct: 181 ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM 240

Query: 261 TNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLPSLMKGEE-SLYKEFTWFEYKR 320
           TNGRFKSVKHRVLTN  KSRVSMIYFGGPPLSEKIAPL SLM+GEE SLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR 300

Query: 321 SAYNSRLADNRLVHFERIAAS 341
           SAYNSRLADNRLV FERIAAS
Sbjct: 301 SAYNSRLADNRLVPFERIAAS 321

[Close]
BLAST of CmaCh04G006490 vs. TrEMBL
Match: Q8GUE8_CUCMA (Gibberellin 2-oxidase OS=Cucurbita maxima GN=ga2ox PE=2 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 1.0e-165
Identity = 297/321 (92.52%), Postives = 305/321 (95.02%), Query Frame = 1 

Query: 21  MAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISTLESESTK 80
           MAAA S SAAF+SG+PLIDLSAPDAK LIVKACEELGFFKVV HGVPME IS+LESESTK
Sbjct: 1   MAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTK 60

Query: 81  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESKSDGFLSIFGQDPQK 140
           FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLES SDGFLS+FGQDPQK
Sbjct: 61  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSMFGQDPQK 120

Query: 141 LRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPELH 200
           LRSAVNDYISAVRNMA EILELMAEGLKIQQRN FSKL+MDE+SDSVFRVNHYPPCP+L 
Sbjct: 121 LRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQ 180

Query: 201 ALKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQSSFFINVGDSLQVM 260
           ALKGTNMIGFGEHTDPQIISVLRSNNTSG QISLADGNWISVPPD SSFFINVGDSLQVM
Sbjct: 181 ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM 240

Query: 261 TNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLPSLMKGEE-SLYKEFTWFEYKR 320
           TNGRFKSVKHRVLTN  KSRVSMIYFGGPPLSEKIAPL SLM+GEE SLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR 300

Query: 321 SAYNSRLADNRLVHFERIAAS 341
           SAYNSRLADNRLV FERIAAS
Sbjct: 301 SAYNSRLADNRLVPFERIAAS 321

[Close]
BLAST of CmaCh04G006490 vs. TrEMBL
Match: O04162_9ROSI (Dioxygenase OS=Marah macrocarpus PE=2 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 1.0e-162
Identity = 298/341 (87.39%), Postives = 308/341 (90.32%), Query Frame = 1 

Query: 1   MVVLSKPSIKQMASSYMRNSMAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFK 60
           MVVLSKP I+Q++SS                 SGVPLIDLSAPDAKHLIVKACEELGFFK
Sbjct: 1   MVVLSKPEIQQLSSSSS---------------SGVPLIDLSAPDAKHLIVKACEELGFFK 60

Query: 61  VVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLN 120
           V+NHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLL 
Sbjct: 61  VINHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLL- 120

Query: 121 THLESKSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLM 180
              +S S  FLSIFGQDPQKLRSA+NDYI AVRNMACEI+ELMAEGLKIQQRNA SKLLM
Sbjct: 121 ---QSNSHAFLSIFGQDPQKLRSALNDYIWAVRNMACEIVELMAEGLKIQQRNALSKLLM 180

Query: 181 DEESDSVFRVNHYPPCPE-LHALKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240
            EESDSVFRVNHYPPCPE L AL+GTNMIGFGEHTDPQIISVLRSNNTSGLQISL D NW
Sbjct: 181 GEESDSVFRVNHYPPCPEELQALEGTNMIGFGEHTDPQIISVLRSNNTSGLQISLPDANW 240

Query: 241 ISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLP 300
           ISVPPDQ+SFFINVGDSLQVMTNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSEKIAPLP
Sbjct: 241 ISVPPDQTSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLP 300

Query: 301 SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 341
           SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 SLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 322

[Close]
BLAST of CmaCh04G006490 vs. TAIR10
Match: AT1G78440.1 (AT1G78440.1 Arabidopsis thaliana gibberellin 2-oxidase 1)

HSP 1 Score: 386.3 bits (991), Expect = 1.9e-107
Identity = 193/309 (62.46%), Postives = 235/309 (76.05%), Query Frame = 1 

Query: 32  FSGVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISTLESESTKFFSLPLSEK-Q 91
           FS +P+ID+S P++KH +VKACE+ GFFKV+NHGV  E +S LE E+  FFSLP SEK Q
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 92  RAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESKSDGFLSIFGQDPQKLRSAVNDYIS 151
            AG   PFGYGN +IGRNGDVGWVEYLL+N + +S S        + P   R+A+ +Y +
Sbjct: 75  VAG--YPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTT 134

Query: 152 AVRNMACEILELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPELHALK--GTNMI 211
           +VR M  ++LE + +GL I+ RN  SKL+ D+ +DS+ R+NHYPPCP  +     G N+I
Sbjct: 135 SVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVI 194

Query: 212 GFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQSSFFINVGDSLQVMTNGRFKSV 271
           GFGEHTDPQIISVLRSNNTSGLQI+L DG+WISVPPD +SFF NVGDSLQVMTNGRFKSV
Sbjct: 195 GFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSV 254

Query: 272 KHRVLTNRLKSRVSMIYFGGPPLSEKIAPLPSLMKGE-ESLYKEFTWFEYKRSAYNSRLA 331
           +HRVL N  KSRVSMIYF GP L+++IAPL  L+  E E LY+EFTW EYK S YNSRL+
Sbjct: 255 RHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLS 314

Query: 332 DNRLVHFER 337
           DNRL  FER
Sbjct: 315 DNRLQQFER 321

[Close]
BLAST of CmaCh04G006490 vs. TAIR10
Match: AT1G30040.1 (AT1G30040.1 gibberellin 2-oxidase)

HSP 1 Score: 374.0 bits (959), Expect = 9.7e-104
Identity = 181/303 (59.74%), Postives = 231/303 (76.24%), Query Frame = 1 

Query: 35  VPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQRAGP 94
           +P+++L+ P+AK  IVKACEE GFFKVVNHGV  E ++ LE E+  FF LP S K RAGP
Sbjct: 31  IPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGP 90

Query: 95  PSPFGYGNKQIGRNGDVGWVEYLLLNTHLESKSDGFLSIFGQDPQKLRSAVNDYISAVRN 154
           P P+GYGNK+IG NGDVGW+EYLLLN + +  S    ++F Q PQ  R +V +Y+  ++ 
Sbjct: 91  PEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEIKE 150

Query: 155 MACEILELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPELHALKGTNMIGFGEHT 214
           ++ ++LE++AE L I+ R+  SK+L DE+SDS  R+NHYP   E    +    +GFGEHT
Sbjct: 151 VSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEE--EAEKMVKVGFGEHT 210

Query: 215 DPQIISVLRSNNTSGLQISLADGNWISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLT 274
           DPQIISVLRSNNT+GLQI + DG+W++VPPD SSFFINVGD+LQVMTNGRFKSVKHRVL 
Sbjct: 211 DPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVKHRVLA 270

Query: 275 NRLKSRVSMIYFGGPPLSEKIAPLPSLMKGEES-LYKEFTWFEYKRSAYNSRLADNRLVH 334
           +  +SR+SMIYFGGPPLS+KIAPLP L+  ++  LYKEFTW +YK SAY S+L D RL  
Sbjct: 271 DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGL 330

Query: 335 FER 337
           FE+
Sbjct: 331 FEK 331

[Close]
BLAST of CmaCh04G006490 vs. TAIR10
Match: AT2G34555.1 (AT2G34555.1 gibberellin 2-oxidase 3)

HSP 1 Score: 344.0 bits (881), Expect = 1.1e-94
Identity = 171/303 (56.44%), Postives = 219/303 (72.28%), Query Frame = 1 

Query: 35  VPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQRAGP 94
           +P+IDL+  DAK  IVKACEE GFFKV+NHGV  + ++ LE E+  FF+L  S K +AGP
Sbjct: 27  IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGP 86

Query: 95  PSPFGYGNKQIGRNGDVGWVEYLLLNTHLESKSDGFLSIFGQDPQKLRSAVNDYISAVRN 154
           P PFGYG K+IG NGD+GW+EY+LLN +L  +S    +IF   P   R AV +YI  ++ 
Sbjct: 87  PDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKR 146

Query: 155 MACEILELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPELHALKGTNMIGFGEHT 214
           M+ + LE++ E LKI+ +   S+L+  +ESDS  R+NHYP   E    +    IGFGEHT
Sbjct: 147 MSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYPEKEETPVKEE---IGFGEHT 206

Query: 215 DPQIISVLRSNNTSGLQISLADGNWISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLT 274
           DPQ+IS+LRSN+T GLQI + DG W+ V PD SSFF+ VGD+LQVMTNGRFKSVKHRV+T
Sbjct: 207 DPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVT 266

Query: 275 NRLKSRVSMIYFGGPPLSEKIAPLPSLM-KGEESLYKEFTWFEYKRSAYNSRLADNRLVH 334
           N  +SR+SMIYF GPPLSEKIAPL  L+ K ++ LY EFTW +YK SAY ++L D RL  
Sbjct: 267 NTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGL 326

Query: 335 FER 337
           FE+
Sbjct: 327 FEK 326

[Close]
BLAST of CmaCh04G006490 vs. TAIR10
Match: AT1G02400.1 (AT1G02400.1 gibberellin 2-oxidase 6)

HSP 1 Score: 303.5 bits (776), Expect = 1.6e-82
Identity = 158/307 (51.47%), Postives = 202/307 (65.80%), Query Frame = 1 

Query: 36  PLIDLSAPDAKHL---IVKACEELGFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQRA 95
           P+ID S  D   L   IVKACE  GFFKV+NHGV  E I   E E  +FF+ P S+K RA
Sbjct: 25  PVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEHEGEEFFNKPESDKLRA 84

Query: 96  GPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESKSDGFLSIFGQDPQKLRSAVNDYISAV 155
           GP SPFGYG K IG NGD+G +EYLLL+ +  + +D   +I   DP K  SA NDYI  V
Sbjct: 85  GPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHDDPFKFSSATNDYIRTV 144

Query: 156 RNMACEILELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPELHALKGTNMIGFGE 215
           R++ACEI++L  E L  Q+ +  S+L+ D  SDS+ R+NHYPP P  +AL G   IGFGE
Sbjct: 145 RDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPAP--YALSGVGQIGFGE 204

Query: 216 HTDPQIISVLRSNNTSGLQISLADGNWISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRV 275
           H+DPQI++VLRSN+  GL+I   DG WI +P D + FF+ VGD LQ +TNGRF SV+HRV
Sbjct: 205 HSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVLVGDCLQALTNGRFTSVRHRV 264

Query: 276 LTNRLKS-RVSMIYFGGPPLSEKIAPLPSLMKGEE-SLYKEFTWFEYKRSAYNSRLADNR 335
           L N  K  R+S +YF  PPL  KI+PLP ++  E    Y  FTW +YK++ Y+ RL   R
Sbjct: 265 LANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYNSFTWGDYKKATYSLRLDVPR 324

Query: 336 LVHFERI 338
           L  F+ +
Sbjct: 325 LEFFKTL 329

[Close]
BLAST of CmaCh04G006490 vs. TAIR10
Match: AT1G47990.1 (AT1G47990.1 gibberellin 2-oxidase 4)

HSP 1 Score: 275.4 bits (703), Expect = 4.7e-74
Identity = 158/326 (48.47%), Postives = 201/326 (61.66%), Query Frame = 1 

Query: 21  MAAAGSSSAAFFSGVPLIDLSAPDAK--HLIVKACEELGFFKVVNHGVPMEFISTLESES 80
           M        A    +P+ID+S   ++    IVKACE LGFFKV+NHGV    IS +E ES
Sbjct: 1   MVKGSQKIVAVDQDIPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQES 60

Query: 81  TKFFSLPLSEKQRAGPPS-PFGYGNKQIGRNGDVGWVEYLLLNTHLESKSDGFLSIFGQD 140
             FF+ P  EK+   P + PF YG + IG NGD G VEYLL +T+       F S     
Sbjct: 61  INFFAKPAHEKKSVRPVNQPFRYGFRDIGLNGDSGEVEYLLFHTN----DPAFRSQLS-- 120

Query: 141 PQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCP 200
                SAVN YI AV+ +A EIL+L AEGL +   ++FS+L+   +SDSV RVNHYPP  
Sbjct: 121 ---FSSAVNCYIEAVKQLAREILDLTAEGLHVPP-HSFSRLISSVDSDSVLRVNHYPPSD 180

Query: 201 ELHALKGTN-------MIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQSSFF 260
           +       +        +GFGEHTDPQI++VLRSN   GLQ+S +DG W+SV PD S+F 
Sbjct: 181 QFFGEANLSDQSVSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFC 240

Query: 261 INVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLPSLM--KGEESL 320
           +NVGD LQVMTNGRF SV+HR LT   +SR+S  YF GPPL  KI PL +++    +  L
Sbjct: 241 VNVGDLLQVMTNGRFISVRHRALTYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRL 300

Query: 321 YKEFTWFEYKRSAYNSRLADNRLVHF 335
           Y+ FTW EYK+ AY+ RL D+RL  F
Sbjct: 301 YQTFTWGEYKKRAYSLRLEDSRLDMF 316

[Close]
BLAST of CmaCh04G006490 vs. NCBI nr
Match: gi|659101776|ref|XP_008451787.1| (PREDICTED: gibberellin 2-beta-dioxygenase 1 [Cucumis melo])

HSP 1 Score: 627.1 bits (1616), Expect = 1.8e-176
Identity = 316/342 (92.40%), Postives = 330/342 (96.49%), Query Frame = 1 

Query: 1   MVVLSKPSIKQMAS-SYMRNSMAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFF 60
           MVVLSK SI+Q++S S+MRNSMA+A SSSAAFFS VPLIDLS+PDAK LIVKACEELGFF
Sbjct: 1   MVVLSKQSIQQLSSNSFMRNSMASAASSSAAFFSEVPLIDLSSPDAKQLIVKACEELGFF 60

Query: 61  KVVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLL 120
           KVVNHGVPMEFISTLESEST FFSLPLSEKQ+A PPSPFGYGNKQIGRNGDVGWVEY+LL
Sbjct: 61  KVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILL 120

Query: 121 NTHLESKSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLL 180
           NTHLES SDGFLSIFGQDPQKLRSAVN+YISAVRNMACEILELMAEGLKIQQRNAFSKL+
Sbjct: 121 NTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNAFSKLV 180

Query: 181 MDEESDSVFRVNHYPPCPELHALKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240
           MDEESDSVFRVNHYPPCP++ ALKG NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW
Sbjct: 181 MDEESDSVFRVNHYPPCPQIQALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240

Query: 241 ISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLP 300
           ISVPPDQ+SFFINVGDSLQVMTNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSEKIAPLP
Sbjct: 241 ISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLP 300

Query: 301 SLMKGEE-SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 341
           SLMKGEE SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 SLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 342

[Close]
BLAST of CmaCh04G006490 vs. NCBI nr
Match: gi|700198381|gb|KGN53539.1| (hypothetical protein Csa_4G075200 [Cucumis sativus])

HSP 1 Score: 620.9 bits (1600), Expect = 1.3e-174
Identity = 312/342 (91.23%), Postives = 327/342 (95.61%), Query Frame = 1 

Query: 1   MVVLSKPSIKQMAS-SYMRNSMAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFF 60
           MV+LSK SI+Q++S S+MRNSMA+A SSS AF S VPLIDLS+PDAK LIVKACEELGFF
Sbjct: 1   MVILSKQSIQQLSSNSFMRNSMASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFF 60

Query: 61  KVVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLL 120
           KVVNHGVPMEFISTLESEST FFSLPLSEKQ+A PPSPFGYGNKQIGRNGDVGWVEY+LL
Sbjct: 61  KVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILL 120

Query: 121 NTHLESKSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLL 180
           NTHLES SDGFLSIFGQDPQKLRSAVN+YISAVRNMACEILELMAEGLKIQQRN FSKL+
Sbjct: 121 NTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLV 180

Query: 181 MDEESDSVFRVNHYPPCPELHALKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240
           MDEESDSVFRVNHYPPCP++ ALKG NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW
Sbjct: 181 MDEESDSVFRVNHYPPCPQIQALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNW 240

Query: 241 ISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLP 300
           ISVPPDQ+SFFINVGDSLQVMTNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSEKIAPLP
Sbjct: 241 ISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLP 300

Query: 301 SLMKGEE-SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 341
           SLMKGEE SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 SLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 342

[Close]
BLAST of CmaCh04G006490 vs. NCBI nr
Match: gi|793421968|ref|NP_001292640.1| (gibberellin 2-beta-dioxygenase 1 [Cucumis sativus])

HSP 1 Score: 599.7 bits (1545), Expect = 3.1e-168
Identity = 298/321 (92.83%), Postives = 308/321 (95.95%), Query Frame = 1 

Query: 21  MAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISTLESESTK 80
           MA+A SSS AF S VPLIDLS+PDAK LIVKACEELGFFKVVNHGVPMEFISTLESEST 
Sbjct: 1   MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60

Query: 81  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESKSDGFLSIFGQDPQK 140
           FFSLPLSEKQ+A PPSPFGYGNKQIGRNGDVGWVEY+LLNTHLES SDGFLSIFGQDPQK
Sbjct: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120

Query: 141 LRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPELH 200
           LRSAVN+YISAVRNMACEILELMAEGLKIQQRN FSKL+MDEESDSVFRVNHYPPCP++ 
Sbjct: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 180

Query: 201 ALKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQSSFFINVGDSLQVM 260
           ALKG NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQ+SFFINVGDSLQVM
Sbjct: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240

Query: 261 TNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLPSLMKGEE-SLYKEFTWFEYKR 320
           TNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSEKIAPLPSLMKGEE SLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300

Query: 321 SAYNSRLADNRLVHFERIAAS 341
           SAYNSRLADNRLVHFERIAAS
Sbjct: 301 SAYNSRLADNRLVHFERIAAS 321

[Close]
BLAST of CmaCh04G006490 vs. NCBI nr
Match: gi|32127337|emb|CAC85924.1| (Gibberellin 2-oxidase [Cucurbita maxima])

HSP 1 Score: 592.0 bits (1525), Expect = 6.4e-166
Identity = 298/321 (92.83%), Postives = 305/321 (95.02%), Query Frame = 1 

Query: 21  MAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISTLESESTK 80
           MAAA S SAAF+SG+PLIDLSAPDAK LIVKACEELGFFKVV HGVPME IS+LESESTK
Sbjct: 1   MAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTK 60

Query: 81  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESKSDGFLSIFGQDPQK 140
           FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLES SDGFLSIFGQDPQK
Sbjct: 61  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQK 120

Query: 141 LRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPELH 200
           LRSAVNDYISAVRNMA EILELMAEGLKIQQRN FSKL+MDE+SDSVFRVNHYPPCP+L 
Sbjct: 121 LRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQ 180

Query: 201 ALKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQSSFFINVGDSLQVM 260
           ALKGTNMIGFGEHTDPQIISVLRSNNTSG QISLADGNWISVPPD SSFFINVGDSLQVM
Sbjct: 181 ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM 240

Query: 261 TNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLPSLMKGEE-SLYKEFTWFEYKR 320
           TNGRFKSVKHRVLTN  KSRVSMIYFGGPPLSEKIAPL SLM+GEE SLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR 300

Query: 321 SAYNSRLADNRLVHFERIAAS 341
           SAYNSRLADNRLV FERIAAS
Sbjct: 301 SAYNSRLADNRLVPFERIAAS 321

[Close]
BLAST of CmaCh04G006490 vs. NCBI nr
Match: gi|27123665|emb|CAC83090.1| (gibberellin 2-oxidase [Cucurbita maxima])

HSP 1 Score: 590.9 bits (1522), Expect = 1.4e-165
Identity = 297/321 (92.52%), Postives = 305/321 (95.02%), Query Frame = 1 

Query: 21  MAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISTLESESTK 80
           MAAA S SAAF+SG+PLIDLSAPDAK LIVKACEELGFFKVV HGVPME IS+LESESTK
Sbjct: 1   MAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTK 60

Query: 81  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESKSDGFLSIFGQDPQK 140
           FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLES SDGFLS+FGQDPQK
Sbjct: 61  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSMFGQDPQK 120

Query: 141 LRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPELH 200
           LRSAVNDYISAVRNMA EILELMAEGLKIQQRN FSKL+MDE+SDSVFRVNHYPPCP+L 
Sbjct: 121 LRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQ 180

Query: 201 ALKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQSSFFINVGDSLQVM 260
           ALKGTNMIGFGEHTDPQIISVLRSNNTSG QISLADGNWISVPPD SSFFINVGDSLQVM
Sbjct: 181 ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM 240

Query: 261 TNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLPSLMKGEE-SLYKEFTWFEYKR 320
           TNGRFKSVKHRVLTN  KSRVSMIYFGGPPLSEKIAPL SLM+GEE SLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR 300

Query: 321 SAYNSRLADNRLVHFERIAAS 341
           SAYNSRLADNRLV FERIAAS
Sbjct: 301 SAYNSRLADNRLVPFERIAAS 321

The following BLAST results are available for this feature:
BLAST of CmaCh04G006490 vs. Swiss-Prot
Match NameE-valueIdentityDescription
G2OX1_PEA4.2e-13369.71Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum GN=GA2OX1 PE=1 SV=1[more]
G2OX_PHACN6.7e-12364.71Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus GN=GA2OX1 PE=2 SV=1[more]
G2OX1_ARATH3.4e-10662.46Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA2OX1 PE=2 SV=2[more]
G2OX2_ARATH1.7e-10259.74Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana GN=GA2OX2 PE=2 SV=1[more]
G2OX3_ARATH1.9e-9356.44Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana GN=GA2OX3 PE=2 SV=1[more]
BLAST of CmaCh04G006490 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KXI6_CUCSA9.0e-17591.23Uncharacterized protein OS=Cucumis sativus GN=Csa_4G075200 PE=3 SV=1[more]
S0BDZ9_CUCSA2.2e-16892.83GA 2-oxidase OS=Cucumis sativus GN=GA2ox1 PE=2 SV=1[more]
Q7X9N5_CUCMA4.5e-16692.83Gibberellin 2-oxidase OS=Cucurbita maxima GN=ga2ox PE=2 SV=1[more]
Q8GUE8_CUCMA1.0e-16592.52Gibberellin 2-oxidase OS=Cucurbita maxima GN=ga2ox PE=2 SV=1[more]
O04162_9ROSI1.0e-16287.39Dioxygenase OS=Marah macrocarpus PE=2 SV=1[more]
BLAST of CmaCh04G006490 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G78440.11.9e-10762.46 Arabidopsis thaliana gibberellin 2-oxidase 1[more]
AT1G30040.19.7e-10459.74 gibberellin 2-oxidase[more]
AT2G34555.11.1e-9456.44 gibberellin 2-oxidase 3[more]
AT1G02400.11.6e-8251.47 gibberellin 2-oxidase 6[more]
AT1G47990.14.7e-7448.47 gibberellin 2-oxidase 4[more]
BLAST of CmaCh04G006490 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659101776|ref|XP_008451787.1|1.8e-17692.40PREDICTED: gibberellin 2-beta-dioxygenase 1 [Cucumis melo][more]
gi|700198381|gb|KGN53539.1|1.3e-17491.23hypothetical protein Csa_4G075200 [Cucumis sativus][more]
gi|793421968|ref|NP_001292640.1|3.1e-16892.83gibberellin 2-beta-dioxygenase 1 [Cucumis sativus][more]
gi|32127337|emb|CAC85924.1|6.4e-16692.83Gibberellin 2-oxidase [Cucurbita maxima][more]
gi|27123665|emb|CAC83090.1|1.4e-16592.52gibberellin 2-oxidase [Cucurbita maxima][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002283Isopenicillin N synthase
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR026992DIOX_N
IPR027443IPNS-like
Vocabulary: Molecular Function
TermDefinition
GO:0005506iron ion binding
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0016491 oxidoreductase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G006490.1CmaCh04G006490.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002283Isopenicillin N synthasePRINTSPR00682IPNSYNTHASEcoord: 213..234
score: 3.3E-9coord: 49..66
score: 3.3E-9coord: 249..275
score: 3.
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 185..287
score: 2.1
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 183..289
score: 1
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 35..126
score: 1.3
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 9..332
score: 1.4
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 1..331
score: 1.0E
NoneNo IPR availablePANTHERPTHR10209:SF91GIBBERELLIN 2-BETA-DIOXYGENASE 1coord: 1..331
score: 1.0E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 9..328
score: 1.72