CmaCh16G004530 (gene) Cucurbita maxima (Rimu)

Overview
NameCmaCh16G004530
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionGibberellin 2-oxidase
LocationCma_Chr16 : 2269857 .. 2271606 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTTCTTCCTCTGTTTCTTTAAAGAAGAAGGAGAAAAAGGGAAACTTTTCATACATTCTTGTTGTTGTTGTTCTTCTTCTTCTCTCAAAACTTTCCAGCAATCCAACAGTTGTCTTTTGATTCCTTAATGAGAAGCTCCACGTCCATGGCGGCTGCTTCTTCTTTCTCTGCAGCATTCTACTCTGGGATTCCATTGATAGACCTCTCTGCACCAGATGCTAAACAACTCATTGTCAAAGCTTGTGAAGAACTCGGATTCTTTAAGGTTGTCAAACATGGTGTCCCCATGGAATTAATCTCCTCTCTTGAATCAGAATCCACCAAATTCTTCTCCCTTCCCCTCTCTGAAAAACAGAGAGCTGGCCCTCCTTCCCCTTTTGGCTATGGAAACAAACAAATTGGCCGCAATGGCGATGTCGGTTGGGTTGAATATCTCCTCTTGAACACTCATCTCGAATCCAACTCCGATGGGTTCCTCTCCATTTTCGGCCAAGACCCACAAAAACTCCGGTAACCAAAACACCCAAATCCCCATTTTCCCAGAATGCTCTATTTTTCACTCTGTTTCTTATACTCTGTTTTTTTTCCTCTCTCTGTTCTCTTTTTCTACGTTTTAGCTCTGCTGTGAACGATTACATCTCGGCTGTGAGGAACATGGCGGGTGAAATCCTCGAGCTAATGGCGGAAGGGTTGAAGATTCAACAACGAAACGTGTTCAGTAAGCTCGTGATGGATGAACAGAGCGACTCTGTTTTCAGAGTGAACCATTATCCGCCATGTCCAGACCTTCAAGCTTTAAAAGGAACAAACATGATTGGATTTGGAGAACACACAGACCCTCAAATCATTTCAGTTTTGAGATCAAACAACACTTCTGGATTTCAAATCTCTCTCGCAGATGGGAATTGGATATCTGTTCCTCCCGATCACAGCTCCTTCTTCATCAATGTTGGTGACTCTTTACAGGTACAATAAACACGACAGCCTCTGTTCTTGATAATCAAAGAAGATTCAAAATTTGAGGTTTTTCCCATCTGGGTTTTCCAAAAAAACATCACCCACCATGTTCTTCATTAAAATAATGAAACTTTTTGTGTTGATGAAGGTGATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGGGTTTTGACAAACAGCTCGAAGTCAAGGGTTTCAATGATCTACTTCGGTGGGCCACCGTTGAGTGAAAAGATAGCTCCTTTAGCTTCCCTTATGCAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACATGGTTTGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCTGACAACAGGCTTGTGCCCTTTGAAAGAATTGCAGCCTCATAATCTTTATCCAAAATTAGGGTCGGAGATGTTCGGATAGTAGTTCGGTTCAACAACGTATGAACGTGTCGAGATGAACTTTCGGTCTCTAACTGTTAGCTTTTGGATAGTTTAGTTCAACGATAAATAGAGGTGACAGGATTCGAACATCTGATCTGTTAGTTAGTTAACTAATCAGTGAGTGTTTTTCTTTTTAGTTTGTTATCCGTGTAGTCTTCTTAATACAAAAGGTATTACTGTTTTTTCTTTTGGGTATTATGAGTTGTCAAAAATAGTACAACACGTTGGTGTTTGTAAGAAACGTGGGAGTTCTCGAAAAGAAGGGTTGTTGTCTCGAGATGTATATTTGTGAGAAACGAGGATGTGTAAGTAATAGCCAATGTGTGTTGTCAAAA

mRNA sequence

TTCTTCTTCCTCTGTTTCTTTAAAGAAGAAGGAGAAAAAGGGAAACTTTTCATACATTCTTGTTGTTGTTGTTCTTCTTCTTCTCTCAAAACTTTCCAGCAATCCAACAGTTGTCTTTTGATTCCTTAATGAGAAGCTCCACGTCCATGGCGGCTGCTTCTTCTTTCTCTGCAGCATTCTACTCTGGGATTCCATTGATAGACCTCTCTGCACCAGATGCTAAACAACTCATTGTCAAAGCTTGTGAAGAACTCGGATTCTTTAAGGTTGTCAAACATGGTGTCCCCATGGAATTAATCTCCTCTCTTGAATCAGAATCCACCAAATTCTTCTCCCTTCCCCTCTCTGAAAAACAGAGAGCTGGCCCTCCTTCCCCTTTTGGCTATGGAAACAAACAAATTGGCCGCAATGGCGATGTCGGTTGGGTTGAATATCTCCTCTTGAACACTCATCTCGAATCCAACTCCGATGGGTTCCTCTCCATTTTCGGCCAAGACCCACAAAAACTCCGCTCTGCTGTGAACGATTACATCTCGGCTGTGAGGAACATGGCGGGTGAAATCCTCGAGCTAATGGCGGAAGGGTTGAAGATTCAACAACGAAACGTGTTCAGTAAGCTCGTGATGGATGAACAGAGCGACTCTGTTTTCAGAGTGAACCATTATCCGCCATGTCCAGACCTTCAAGCTTTAAAAGGAACAAACATGATTGGATTTGGAGAACACACAGACCCTCAAATCATTTCAGTTTTGAGATCAAACAACACTTCTGGATTTCAAATCTCTCTCGCAGATGGGAATTGGATATCTGTTCCTCCCGATCACAGCTCCTTCTTCATCAATGTTGGTGACTCTTTACAGGTGATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGGGTTTTGACAAACAGCTCGAAGTCAAGGGTTTCAATGATCTACTTCGGTGGGCCACCGTTGAGTGAAAAGATAGCTCCTTTAGCTTCCCTTATGCAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACATGGTTTGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCTGACAACAGGCTTGTGCCCTTTGAAAGAATTGCAGCCTCATAATCTTTATCCAAAATTAGGGTCGGAGATGTTCGGATAGTAGTTCGGTTCAACAACGTATGAACGTGTCGAGATGAACTTTCGGTCTCTAACTGTTAGCTTTTGGATAGTTTAGTTCAACGATAAATAGAGGTGACAGGATTCGAACATCTGATCTGTTAGTTAGTTAACTAATCAGTGAGTGTTTTTCTTTTTAGTTTGTTATCCGTGTAGTCTTCTTAATACAAAAGGTATTACTGTTTTTTCTTTTGGGTATTATGAGTTGTCAAAAATAGTACAACACGTTGGTGTTTGTAAGAAACGTGGGAGTTCTCGAAAAGAAGGGTTGTTGTCTCGAGATGTATATTTGTGAGAAACGAGGATGTGTAAGTAATAGCCAATGTGTGTTGTCAAAA

Coding sequence (CDS)

ATGAGAAGCTCCACGTCCATGGCGGCTGCTTCTTCTTTCTCTGCAGCATTCTACTCTGGGATTCCATTGATAGACCTCTCTGCACCAGATGCTAAACAACTCATTGTCAAAGCTTGTGAAGAACTCGGATTCTTTAAGGTTGTCAAACATGGTGTCCCCATGGAATTAATCTCCTCTCTTGAATCAGAATCCACCAAATTCTTCTCCCTTCCCCTCTCTGAAAAACAGAGAGCTGGCCCTCCTTCCCCTTTTGGCTATGGAAACAAACAAATTGGCCGCAATGGCGATGTCGGTTGGGTTGAATATCTCCTCTTGAACACTCATCTCGAATCCAACTCCGATGGGTTCCTCTCCATTTTCGGCCAAGACCCACAAAAACTCCGCTCTGCTGTGAACGATTACATCTCGGCTGTGAGGAACATGGCGGGTGAAATCCTCGAGCTAATGGCGGAAGGGTTGAAGATTCAACAACGAAACGTGTTCAGTAAGCTCGTGATGGATGAACAGAGCGACTCTGTTTTCAGAGTGAACCATTATCCGCCATGTCCAGACCTTCAAGCTTTAAAAGGAACAAACATGATTGGATTTGGAGAACACACAGACCCTCAAATCATTTCAGTTTTGAGATCAAACAACACTTCTGGATTTCAAATCTCTCTCGCAGATGGGAATTGGATATCTGTTCCTCCCGATCACAGCTCCTTCTTCATCAATGTTGGTGACTCTTTACAGGTGATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGGGTTTTGACAAACAGCTCGAAGTCAAGGGTTTCAATGATCTACTTCGGTGGGCCACCGTTGAGTGAAAAGATAGCTCCTTTAGCTTCCCTTATGCAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACATGGTTTGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCTGACAACAGGCTTGTGCCCTTTGAAAGAATTGCAGCCTCATAA

Protein sequence

MRSSTSMAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRLVPFERIAAS
Homology
[Close]
BLAST of CmaCh16G004530 vs. Swiss-Prot
Match: G2OX1_PEA (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 1.8e-128
Identity = 227/311 (72.99%), Postives = 261/311 (83.92%), Query Frame = 1 

Query: 17  FYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKFFSLPLSEKQ 76
           F S IPL+DLS PDAK LIVKACE+ GFFKV+ HG+P++ IS LESE+ KFFSLP +EK+
Sbjct: 23  FSSSIPLVDLSKPDAKTLIVKACEDFGFFKVINHGIPLDAISQLESEAFKFFSLPQTEKE 82

Query: 77  RAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYIS 136
           +AGP +PFGYGNK+IG NGD+GW+EYLLL T    N D   S++G+D  K R  + DY  
Sbjct: 83  KAGPANPFGYGNKRIGLNGDIGWIEYLLLTT----NQDHNFSLYGEDIDKFRGLLKDYKC 142

Query: 137 AVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGF 196
           A+RNMA EIL+LMAEGLKIQ +NVFSKLVMD+QSD +FRVNHYP CP+L A+ G N+IGF
Sbjct: 143 AMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLFRVNHYPACPEL-AINGENLIGF 202

Query: 197 GEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKH 256
           GEHTDPQIIS+LRSNNTSGFQISL DG+WISVPPDHSSFFINVGDSLQVMTNGRFKSV+H
Sbjct: 203 GEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSSFFINVGDSLQVMTNGRFKSVRH 262

Query: 257 RVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADN 316
           RVL N    R+SMIYF GPPLSEKIAPL SLM+G+E SLYKEFTWFEYK S Y SRLADN
Sbjct: 263 RVLANGIDPRLSMIYFCGPPLSEKIAPLPSLMKGKE-SLYKEFTWFEYKSSTYGSRLADN 322

Query: 317 RLVPFERIAAS 328
           RL  +ERIAA+
Sbjct: 323 RLGNYERIAAT 327

[Close]
BLAST of CmaCh16G004530 vs. Swiss-Prot
Match: G2OX_PHACN (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 6.7e-120
Identity = 207/313 (66.13%), Postives = 254/313 (81.15%), Query Frame = 1 

Query: 14  SAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKFFSLPLS 73
           S   ++GIP++DL+ PDAK LIV AC + GFFK+V HGVP+EL+++LE+E+ +FF    S
Sbjct: 20  STPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHGVPLELMANLENEALRFFKKSQS 79

Query: 74  EKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVND 133
           EK RAGPP PFGYG+K+IG NGDVGWVEYLLLNT+ +  S   L IF ++P   R+ V +
Sbjct: 80  EKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVISPKSLCIFRENPHHFRAVVEN 139

Query: 134 YISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNM 193
           YI+AV+NM   +LELMAEGL I+QRN  S+L+ DE+SDS FR+NHYPPCP++QAL   N+
Sbjct: 140 YITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCFRLNHYPPCPEVQAL-NRNL 199

Query: 194 IGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKS 253
           +GFGEHTDPQIISVLRSN+TSG QI L DG W+SVPPD +SFFINVGD+LQVMTNGRFKS
Sbjct: 200 VGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTSFFINVGDALQVMTNGRFKS 259

Query: 254 VKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRL 313
           VKHRVL +++KSR+SMIYFGGP LSE IAPL S+M   E  LYKEFTW EYK++AY SRL
Sbjct: 260 VKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVMLKGEECLYKEFTWCEYKKAAYTSRL 319

Query: 314 ADNRLVPFERIAA 327
           ADNRL PF++ AA
Sbjct: 320 ADNRLAPFQKSAA 331

[Close]
BLAST of CmaCh16G004530 vs. Swiss-Prot
Match: G2OX1_ARATH (Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA2OX1 PE=2 SV=2)

HSP 1 Score: 389.4 bits (999), Expect = 3.8e-107
Identity = 192/309 (62.14%), Postives = 235/309 (76.05%), Query Frame = 1 

Query: 18  YSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKFFSLPLSEK-Q 77
           +S IP+ID+S P++K  +VKACE+ GFFKV+ HGV  EL+S LE E+  FFSLP SEK Q
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 78  RAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYIS 137
            AG   PFGYGN +IGRNGDVGWVEYLL+N + +S S        + P   R+A+ +Y +
Sbjct: 75  VAG--YPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTT 134

Query: 138 AVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCP--DLQALKGTNMI 197
           +VR M  ++LE + +GL I+ RN  SKLV D+ +DS+ R+NHYPPCP  + +   G N+I
Sbjct: 135 SVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVI 194

Query: 198 GFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSV 257
           GFGEHTDPQIISVLRSNNTSG QI+L DG+WISVPPDH+SFF NVGDSLQVMTNGRFKSV
Sbjct: 195 GFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSV 254

Query: 258 KHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLA 317
           +HRVL N  KSRVSMIYF GP L+++IAPL  L+  E+  LY+EFTW EYK S YNSRL+
Sbjct: 255 RHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLS 314

Query: 318 DNRLVPFER 324
           DNRL  FER
Sbjct: 315 DNRLQQFER 321

[Close]
BLAST of CmaCh16G004530 vs. Swiss-Prot
Match: G2OX2_ARATH (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 2.8e-102
Identity = 181/303 (59.74%), Postives = 234/303 (77.23%), Query Frame = 1 

Query: 21  IPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKFFSLPLSEKQRAGP 80
           IP+++L+ P+AK  IVKACEE GFFKVV HGV  EL++ LE E+  FF LP S K RAGP
Sbjct: 31  IPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGP 90

Query: 81  PSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYISAVRN 140
           P P+GYGNK+IG NGDVGW+EYLLLN + + +S    ++F Q PQ  R +V +Y+  ++ 
Sbjct: 91  PEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEIKE 150

Query: 141 MAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFGEHT 200
           ++ ++LE++AE L I+ R+  SK++ DE+SDS  R+NHYP   + +A K    +GFGEHT
Sbjct: 151 VSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEE-EAEKMVK-VGFGEHT 210

Query: 201 DPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRVLT 260
           DPQIISVLRSNNT+G QI + DG+W++VPPDHSSFFINVGD+LQVMTNGRFKSVKHRVL 
Sbjct: 211 DPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVKHRVLA 270

Query: 261 NSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRLVP 320
           ++ +SR+SMIYFGGPPLS+KIAPL  L+  ++  LYKEFTW +YK SAY S+L D RL  
Sbjct: 271 DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGL 330

Query: 321 FER 324
           FE+
Sbjct: 331 FEK 331

[Close]
BLAST of CmaCh16G004530 vs. Swiss-Prot
Match: G2OX3_ARATH (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.3e-94
Identity = 170/303 (56.11%), Postives = 219/303 (72.28%), Query Frame = 1 

Query: 21  IPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKFFSLPLSEKQRAGP 80
           IP+IDL+  DAK  IVKACEE GFFKV+ HGV  +L++ LE E+  FF+L  S K +AGP
Sbjct: 27  IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGP 86

Query: 81  PSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYISAVRN 140
           P PFGYG K+IG NGD+GW+EY+LLN +L   S    +IF   P   R AV +YI  ++ 
Sbjct: 87  PDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKR 146

Query: 141 MAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFGEHT 200
           M+ + LE++ E LKI+ +   S+LV  ++SDS  R+NHYP   + +       IGFGEHT
Sbjct: 147 MSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYP---EKEETPVKEEIGFGEHT 206

Query: 201 DPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRVLT 260
           DPQ+IS+LRSN+T G QI + DG W+ V PDHSSFF+ VGD+LQVMTNGRFKSVKHRV+T
Sbjct: 207 DPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVT 266

Query: 261 NSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRLVP 320
           N+ +SR+SMIYF GPPLSEKIAPL+ L+  ++  LY EFTW +YK SAY ++L D RL  
Sbjct: 267 NTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGL 326

Query: 321 FER 324
           FE+
Sbjct: 327 FEK 326

[Close]
BLAST of CmaCh16G004530 vs. TrEMBL
Match: Q7X9N5_CUCMA (Gibberellin 2-oxidase OS=Cucurbita maxima GN=ga2ox PE=2 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 1.4e-180
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 1 

Query: 7   MAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTK 66
           MAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTK
Sbjct: 1   MAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTK 60

Query: 67  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQK 126
           FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQK
Sbjct: 61  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQK 120

Query: 127 LRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQ 186
           LRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQ
Sbjct: 121 LRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQ 180

Query: 187 ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM 246
           ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM
Sbjct: 181 ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM 240

Query: 247 TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR 306
           TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR 300

Query: 307 SAYNSRLADNRLVPFERIAAS 328
           SAYNSRLADNRLVPFERIAAS
Sbjct: 301 SAYNSRLADNRLVPFERIAAS 321

[Close]
BLAST of CmaCh16G004530 vs. TrEMBL
Match: Q8GUE8_CUCMA (Gibberellin 2-oxidase OS=Cucurbita maxima GN=ga2ox PE=2 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 3.1e-180
Identity = 320/321 (99.69%), Postives = 321/321 (100.00%), Query Frame = 1 

Query: 7   MAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTK 66
           MAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTK
Sbjct: 1   MAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTK 60

Query: 67  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQK 126
           FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLS+FGQDPQK
Sbjct: 61  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSMFGQDPQK 120

Query: 127 LRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQ 186
           LRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQ
Sbjct: 121 LRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQ 180

Query: 187 ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM 246
           ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM
Sbjct: 181 ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM 240

Query: 247 TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR 306
           TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR 300

Query: 307 SAYNSRLADNRLVPFERIAAS 328
           SAYNSRLADNRLVPFERIAAS
Sbjct: 301 SAYNSRLADNRLVPFERIAAS 321

[Close]
BLAST of CmaCh16G004530 vs. TrEMBL
Match: A0A0A0KXI6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G075200 PE=3 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 1.1e-164
Identity = 293/322 (90.99%), Postives = 306/322 (95.03%), Query Frame = 1 

Query: 6   SMAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESEST 65
           SMA+A+S S AF S +PLIDLS+PDAKQLIVKACEELGFFKVV HGVPME IS+LESEST
Sbjct: 21  SMASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESEST 80

Query: 66  KFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQ 125
            FFSLPLSEKQ+A PPSPFGYGNKQIGRNGDVGWVEY+LLNTHLESNSDGFLSIFGQDPQ
Sbjct: 81  NFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQ 140

Query: 126 KLRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDL 185
           KLRSAVN+YISAVRNMA EILELMAEGLKIQQRNVFSKLVMDE+SDSVFRVNHYPPCP +
Sbjct: 141 KLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQI 200

Query: 186 QALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQV 245
           QALKG NMIGFGEHTDPQIISVLRSNNTSG QISLADGNWISVPPD +SFFINVGDSLQV
Sbjct: 201 QALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV 260

Query: 246 MTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYK 305
           MTNGRFKSVKHRVLTNS KSR+SMIYFGGPPLSEKIAPL SLM+GEERSLYKEFTWFEYK
Sbjct: 261 MTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYK 320

Query: 306 RSAYNSRLADNRLVPFERIAAS 328
           RSAYNSRLADNRLV FERIAAS
Sbjct: 321 RSAYNSRLADNRLVHFERIAAS 342

[Close]
BLAST of CmaCh16G004530 vs. TrEMBL
Match: S0BDZ9_CUCSA (GA 2-oxidase OS=Cucumis sativus GN=GA2ox1 PE=2 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 2.4e-164
Identity = 292/321 (90.97%), Postives = 305/321 (95.02%), Query Frame = 1 

Query: 7   MAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTK 66
           MA+A+S S AF S +PLIDLS+PDAKQLIVKACEELGFFKVV HGVPME IS+LESEST 
Sbjct: 1   MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60

Query: 67  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQK 126
           FFSLPLSEKQ+A PPSPFGYGNKQIGRNGDVGWVEY+LLNTHLESNSDGFLSIFGQDPQK
Sbjct: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120

Query: 127 LRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQ 186
           LRSAVN+YISAVRNMA EILELMAEGLKIQQRNVFSKLVMDE+SDSVFRVNHYPPCP +Q
Sbjct: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 180

Query: 187 ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM 246
           ALKG NMIGFGEHTDPQIISVLRSNNTSG QISLADGNWISVPPD +SFFINVGDSLQVM
Sbjct: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240

Query: 247 TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR 306
           TNGRFKSVKHRVLTNS KSR+SMIYFGGPPLSEKIAPL SLM+GEERSLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300

Query: 307 SAYNSRLADNRLVPFERIAAS 328
           SAYNSRLADNRLV FERIAAS
Sbjct: 301 SAYNSRLADNRLVHFERIAAS 321

[Close]
BLAST of CmaCh16G004530 vs. TrEMBL
Match: O04162_9ROSI (Dioxygenase OS=Marah macrocarpus PE=2 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 1.1e-150
Identity = 275/315 (87.30%), Postives = 290/315 (92.06%), Query Frame = 1 

Query: 14  SAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKFFSLPLS 73
           S++  SG+PLIDLSAPDAK LIVKACEELGFFKV+ HGVPME IS+LESESTKFFSLPLS
Sbjct: 13  SSSSSSGVPLIDLSAPDAKHLIVKACEELGFFKVINHGVPMEFISTLESESTKFFSLPLS 72

Query: 74  EKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVND 133
           EKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLL    +SNS  FLSIFGQDPQKLRSA+ND
Sbjct: 73  EKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLL----QSNSHAFLSIFGQDPQKLRSALND 132

Query: 134 YISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPD-LQALKGTN 193
           YI AVRNMA EI+ELMAEGLKIQQRN  SKL+M E+SDSVFRVNHYPPCP+ LQAL+GTN
Sbjct: 133 YIWAVRNMACEIVELMAEGLKIQQRNALSKLLMGEESDSVFRVNHYPPCPEELQALEGTN 192

Query: 194 MIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFK 253
           MIGFGEHTDPQIISVLRSNNTSG QISL D NWISVPPD +SFFINVGDSLQVMTNGRFK
Sbjct: 193 MIGFGEHTDPQIISVLRSNNTSGLQISLPDANWISVPPDQTSFFINVGDSLQVMTNGRFK 252

Query: 254 SVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSR 313
           SVKHRVLTNS KSR+SMIYFGGPPLSEKIAPL SLM+GEE SLYKEFTWFEYKRSAYNSR
Sbjct: 253 SVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEE-SLYKEFTWFEYKRSAYNSR 312

Query: 314 LADNRLVPFERIAAS 328
           LADNRLV FERIAAS
Sbjct: 313 LADNRLVHFERIAAS 322

[Close]
BLAST of CmaCh16G004530 vs. TAIR10
Match: AT1G78440.1 (AT1G78440.1 Arabidopsis thaliana gibberellin 2-oxidase 1)

HSP 1 Score: 389.4 bits (999), Expect = 2.1e-108
Identity = 192/309 (62.14%), Postives = 235/309 (76.05%), Query Frame = 1 

Query: 18  YSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKFFSLPLSEK-Q 77
           +S IP+ID+S P++K  +VKACE+ GFFKV+ HGV  EL+S LE E+  FFSLP SEK Q
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 78  RAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYIS 137
            AG   PFGYGN +IGRNGDVGWVEYLL+N + +S S        + P   R+A+ +Y +
Sbjct: 75  VAG--YPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTT 134

Query: 138 AVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCP--DLQALKGTNMI 197
           +VR M  ++LE + +GL I+ RN  SKLV D+ +DS+ R+NHYPPCP  + +   G N+I
Sbjct: 135 SVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVI 194

Query: 198 GFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSV 257
           GFGEHTDPQIISVLRSNNTSG QI+L DG+WISVPPDH+SFF NVGDSLQVMTNGRFKSV
Sbjct: 195 GFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSV 254

Query: 258 KHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLA 317
           +HRVL N  KSRVSMIYF GP L+++IAPL  L+  E+  LY+EFTW EYK S YNSRL+
Sbjct: 255 RHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLS 314

Query: 318 DNRLVPFER 324
           DNRL  FER
Sbjct: 315 DNRLQQFER 321

[Close]
BLAST of CmaCh16G004530 vs. TAIR10
Match: AT1G30040.1 (AT1G30040.1 gibberellin 2-oxidase)

HSP 1 Score: 373.2 bits (957), Expect = 1.6e-103
Identity = 181/303 (59.74%), Postives = 234/303 (77.23%), Query Frame = 1 

Query: 21  IPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKFFSLPLSEKQRAGP 80
           IP+++L+ P+AK  IVKACEE GFFKVV HGV  EL++ LE E+  FF LP S K RAGP
Sbjct: 31  IPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGP 90

Query: 81  PSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYISAVRN 140
           P P+GYGNK+IG NGDVGW+EYLLLN + + +S    ++F Q PQ  R +V +Y+  ++ 
Sbjct: 91  PEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEIKE 150

Query: 141 MAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFGEHT 200
           ++ ++LE++AE L I+ R+  SK++ DE+SDS  R+NHYP   + +A K    +GFGEHT
Sbjct: 151 VSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEE-EAEKMVK-VGFGEHT 210

Query: 201 DPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRVLT 260
           DPQIISVLRSNNT+G QI + DG+W++VPPDHSSFFINVGD+LQVMTNGRFKSVKHRVL 
Sbjct: 211 DPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVKHRVLA 270

Query: 261 NSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRLVP 320
           ++ +SR+SMIYFGGPPLS+KIAPL  L+  ++  LYKEFTW +YK SAY S+L D RL  
Sbjct: 271 DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGL 330

Query: 321 FER 324
           FE+
Sbjct: 331 FEK 331

[Close]
BLAST of CmaCh16G004530 vs. TAIR10
Match: AT2G34555.1 (AT2G34555.1 gibberellin 2-oxidase 3)

HSP 1 Score: 347.8 bits (891), Expect = 7.2e-96
Identity = 170/303 (56.11%), Postives = 219/303 (72.28%), Query Frame = 1 

Query: 21  IPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKFFSLPLSEKQRAGP 80
           IP+IDL+  DAK  IVKACEE GFFKV+ HGV  +L++ LE E+  FF+L  S K +AGP
Sbjct: 27  IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGP 86

Query: 81  PSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYISAVRN 140
           P PFGYG K+IG NGD+GW+EY+LLN +L   S    +IF   P   R AV +YI  ++ 
Sbjct: 87  PDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKR 146

Query: 141 MAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFGEHT 200
           M+ + LE++ E LKI+ +   S+LV  ++SDS  R+NHYP   + +       IGFGEHT
Sbjct: 147 MSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYP---EKEETPVKEEIGFGEHT 206

Query: 201 DPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRVLT 260
           DPQ+IS+LRSN+T G QI + DG W+ V PDHSSFF+ VGD+LQVMTNGRFKSVKHRV+T
Sbjct: 207 DPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVT 266

Query: 261 NSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRLVP 320
           N+ +SR+SMIYF GPPLSEKIAPL+ L+  ++  LY EFTW +YK SAY ++L D RL  
Sbjct: 267 NTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGL 326

Query: 321 FER 324
           FE+
Sbjct: 327 FEK 326

[Close]
BLAST of CmaCh16G004530 vs. TAIR10
Match: AT1G02400.1 (AT1G02400.1 gibberellin 2-oxidase 6)

HSP 1 Score: 295.0 bits (754), Expect = 5.5e-80
Identity = 154/307 (50.16%), Postives = 202/307 (65.80%), Query Frame = 1 

Query: 22  PLIDLSAPDAKQL---IVKACEELGFFKVVKHGVPMELISSLESESTKFFSLPLSEKQRA 81
           P+ID S  D  +L   IVKACE  GFFKV+ HGV  E+I   E E  +FF+ P S+K RA
Sbjct: 25  PVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEHEGEEFFNKPESDKLRA 84

Query: 82  GPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYISAV 141
           GP SPFGYG K IG NGD+G +EYLLL+ +  + +D   +I   DP K  SA NDYI  V
Sbjct: 85  GPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHDDPFKFSSATNDYIRTV 144

Query: 142 RNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFGE 201
           R++A EI++L  E L  Q+ +  S+L+ D +SDS+ R+NHYPP P   AL G   IGFGE
Sbjct: 145 RDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPAP--YALSGVGQIGFGE 204

Query: 202 HTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRV 261
           H+DPQI++VLRSN+  G +I   DG WI +P D + FF+ VGD LQ +TNGRF SV+HRV
Sbjct: 205 HSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVLVGDCLQALTNGRFTSVRHRV 264

Query: 262 LTNSSKS-RVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNR 321
           L N++K  R+S +YF  PPL  KI+PL  ++  E    Y  FTW +YK++ Y+ RL   R
Sbjct: 265 LANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYNSFTWGDYKKATYSLRLDVPR 324

Query: 322 LVPFERI 325
           L  F+ +
Sbjct: 325 LEFFKTL 329

[Close]
BLAST of CmaCh16G004530 vs. TAIR10
Match: AT1G47990.1 (AT1G47990.1 gibberellin 2-oxidase 4)

HSP 1 Score: 276.2 bits (705), Expect = 2.7e-74
Identity = 163/327 (49.85%), Postives = 200/327 (61.16%), Query Frame = 1 

Query: 7   MAAASSFSAAFYSGIPLIDLSAPDAK--QLIVKACEELGFFKVVKHGVPMELISSLESES 66
           M   S    A    IP+ID+S   ++    IVKACE LGFFKV+ HGV    IS +E ES
Sbjct: 1   MVKGSQKIVAVDQDIPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQES 60

Query: 67  TKFFSLPLSEKQRAGPPS-PFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQD 126
             FF+ P  EK+   P + PF YG + IG NGD G VEYLL +T    N   F S     
Sbjct: 61  INFFAKPAHEKKSVRPVNQPFRYGFRDIGLNGDSGEVEYLLFHT----NDPAFRSQLS-- 120

Query: 127 PQKLRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCP 186
                SAVN YI AV+ +A EIL+L AEGL +   + FS+L+    SDSV RVNHYPP  
Sbjct: 121 ---FSSAVNCYIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSD 180

Query: 187 DL--------QALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSF 246
                     Q++  T  +GFGEHTDPQI++VLRSN   G Q+S +DG W+SV PD S+F
Sbjct: 181 QFFGEANLSDQSVSLTR-VGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAF 240

Query: 247 FINVGDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPL-ASLMQGEERS 306
            +NVGD LQVMTNGRF SV+HR LT   +SR+S  YF GPPL  KI PL A +M   +  
Sbjct: 241 CVNVGDLLQVMTNGRFISVRHRALTYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPR 300

Query: 307 LYKEFTWFEYKRSAYNSRLADNRLVPF 322
           LY+ FTW EYK+ AY+ RL D+RL  F
Sbjct: 301 LYQTFTWGEYKKRAYSLRLEDSRLDMF 316

[Close]
BLAST of CmaCh16G004530 vs. NCBI nr
Match: gi|32127337|emb|CAC85924.1| (Gibberellin 2-oxidase [Cucurbita maxima])

HSP 1 Score: 640.2 bits (1650), Expect = 2.0e-180
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 1 

Query: 7   MAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTK 66
           MAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTK
Sbjct: 1   MAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTK 60

Query: 67  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQK 126
           FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQK
Sbjct: 61  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQK 120

Query: 127 LRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQ 186
           LRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQ
Sbjct: 121 LRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQ 180

Query: 187 ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM 246
           ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM
Sbjct: 181 ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM 240

Query: 247 TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR 306
           TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR 300

Query: 307 SAYNSRLADNRLVPFERIAAS 328
           SAYNSRLADNRLVPFERIAAS
Sbjct: 301 SAYNSRLADNRLVPFERIAAS 321

[Close]
BLAST of CmaCh16G004530 vs. NCBI nr
Match: gi|27123665|emb|CAC83090.1| (gibberellin 2-oxidase [Cucurbita maxima])

HSP 1 Score: 639.0 bits (1647), Expect = 4.4e-180
Identity = 320/321 (99.69%), Postives = 321/321 (100.00%), Query Frame = 1 

Query: 7   MAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTK 66
           MAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTK
Sbjct: 1   MAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTK 60

Query: 67  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQK 126
           FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLS+FGQDPQK
Sbjct: 61  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSMFGQDPQK 120

Query: 127 LRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQ 186
           LRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQ
Sbjct: 121 LRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQ 180

Query: 187 ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM 246
           ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM
Sbjct: 181 ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM 240

Query: 247 TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR 306
           TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR 300

Query: 307 SAYNSRLADNRLVPFERIAAS 328
           SAYNSRLADNRLVPFERIAAS
Sbjct: 301 SAYNSRLADNRLVPFERIAAS 321

[Close]
BLAST of CmaCh16G004530 vs. NCBI nr
Match: gi|659101776|ref|XP_008451787.1| (PREDICTED: gibberellin 2-beta-dioxygenase 1 [Cucumis melo])

HSP 1 Score: 589.3 bits (1518), Expect = 4.0e-165
Identity = 293/322 (90.99%), Postives = 307/322 (95.34%), Query Frame = 1 

Query: 6   SMAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESEST 65
           SMA+A+S SAAF+S +PLIDLS+PDAKQLIVKACEELGFFKVV HGVPME IS+LESEST
Sbjct: 21  SMASAASSSAAFFSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESEST 80

Query: 66  KFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQ 125
            FFSLPLSEKQ+A PPSPFGYGNKQIGRNGDVGWVEY+LLNTHLESNSDGFLSIFGQDPQ
Sbjct: 81  NFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQ 140

Query: 126 KLRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDL 185
           KLRSAVN+YISAVRNMA EILELMAEGLKIQQRN FSKLVMDE+SDSVFRVNHYPPCP +
Sbjct: 141 KLRSAVNNYISAVRNMACEILELMAEGLKIQQRNAFSKLVMDEESDSVFRVNHYPPCPQI 200

Query: 186 QALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQV 245
           QALKG NMIGFGEHTDPQIISVLRSNNTSG QISLADGNWISVPPD +SFFINVGDSLQV
Sbjct: 201 QALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV 260

Query: 246 MTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYK 305
           MTNGRFKSVKHRVLTNS KSR+SMIYFGGPPLSEKIAPL SLM+GEERSLYKEFTWFEYK
Sbjct: 261 MTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYK 320

Query: 306 RSAYNSRLADNRLVPFERIAAS 328
           RSAYNSRLADNRLV FERIAAS
Sbjct: 321 RSAYNSRLADNRLVHFERIAAS 342

[Close]
BLAST of CmaCh16G004530 vs. NCBI nr
Match: gi|700198381|gb|KGN53539.1| (hypothetical protein Csa_4G075200 [Cucumis sativus])

HSP 1 Score: 587.4 bits (1513), Expect = 1.5e-164
Identity = 293/322 (90.99%), Postives = 306/322 (95.03%), Query Frame = 1 

Query: 6   SMAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESEST 65
           SMA+A+S S AF S +PLIDLS+PDAKQLIVKACEELGFFKVV HGVPME IS+LESEST
Sbjct: 21  SMASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESEST 80

Query: 66  KFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQ 125
            FFSLPLSEKQ+A PPSPFGYGNKQIGRNGDVGWVEY+LLNTHLESNSDGFLSIFGQDPQ
Sbjct: 81  NFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQ 140

Query: 126 KLRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDL 185
           KLRSAVN+YISAVRNMA EILELMAEGLKIQQRNVFSKLVMDE+SDSVFRVNHYPPCP +
Sbjct: 141 KLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQI 200

Query: 186 QALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQV 245
           QALKG NMIGFGEHTDPQIISVLRSNNTSG QISLADGNWISVPPD +SFFINVGDSLQV
Sbjct: 201 QALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQV 260

Query: 246 MTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYK 305
           MTNGRFKSVKHRVLTNS KSR+SMIYFGGPPLSEKIAPL SLM+GEERSLYKEFTWFEYK
Sbjct: 261 MTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYK 320

Query: 306 RSAYNSRLADNRLVPFERIAAS 328
           RSAYNSRLADNRLV FERIAAS
Sbjct: 321 RSAYNSRLADNRLVHFERIAAS 342

[Close]
BLAST of CmaCh16G004530 vs. NCBI nr
Match: gi|793421968|ref|NP_001292640.1| (gibberellin 2-beta-dioxygenase 1 [Cucumis sativus])

HSP 1 Score: 586.3 bits (1510), Expect = 3.4e-164
Identity = 292/321 (90.97%), Postives = 305/321 (95.02%), Query Frame = 1 

Query: 7   MAAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTK 66
           MA+A+S S AF S +PLIDLS+PDAKQLIVKACEELGFFKVV HGVPME IS+LESEST 
Sbjct: 1   MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60

Query: 67  FFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQK 126
           FFSLPLSEKQ+A PPSPFGYGNKQIGRNGDVGWVEY+LLNTHLESNSDGFLSIFGQDPQK
Sbjct: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120

Query: 127 LRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQ 186
           LRSAVN+YISAVRNMA EILELMAEGLKIQQRNVFSKLVMDE+SDSVFRVNHYPPCP +Q
Sbjct: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 180

Query: 187 ALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVM 246
           ALKG NMIGFGEHTDPQIISVLRSNNTSG QISLADGNWISVPPD +SFFINVGDSLQVM
Sbjct: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240

Query: 247 TNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKR 306
           TNGRFKSVKHRVLTNS KSR+SMIYFGGPPLSEKIAPL SLM+GEERSLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300

Query: 307 SAYNSRLADNRLVPFERIAAS 328
           SAYNSRLADNRLV FERIAAS
Sbjct: 301 SAYNSRLADNRLVHFERIAAS 321

The following BLAST results are available for this feature:
BLAST of CmaCh16G004530 vs. Swiss-Prot
Match NameE-valueIdentityDescription
G2OX1_PEA1.8e-12872.99Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum GN=GA2OX1 PE=1 SV=1[more]
G2OX_PHACN6.7e-12066.13Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus GN=GA2OX1 PE=2 SV=1[more]
G2OX1_ARATH3.8e-10762.14Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA2OX1 PE=2 SV=2[more]
G2OX2_ARATH2.8e-10259.74Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana GN=GA2OX2 PE=2 SV=1[more]
G2OX3_ARATH1.3e-9456.11Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana GN=GA2OX3 PE=2 SV=1[more]
BLAST of CmaCh16G004530 vs. TrEMBL
Match NameE-valueIdentityDescription
Q7X9N5_CUCMA1.4e-180100.00Gibberellin 2-oxidase OS=Cucurbita maxima GN=ga2ox PE=2 SV=1[more]
Q8GUE8_CUCMA3.1e-18099.69Gibberellin 2-oxidase OS=Cucurbita maxima GN=ga2ox PE=2 SV=1[more]
A0A0A0KXI6_CUCSA1.1e-16490.99Uncharacterized protein OS=Cucumis sativus GN=Csa_4G075200 PE=3 SV=1[more]
S0BDZ9_CUCSA2.4e-16490.97GA 2-oxidase OS=Cucumis sativus GN=GA2ox1 PE=2 SV=1[more]
O04162_9ROSI1.1e-15087.30Dioxygenase OS=Marah macrocarpus PE=2 SV=1[more]
BLAST of CmaCh16G004530 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G78440.12.1e-10862.14 Arabidopsis thaliana gibberellin 2-oxidase 1[more]
AT1G30040.11.6e-10359.74 gibberellin 2-oxidase[more]
AT2G34555.17.2e-9656.11 gibberellin 2-oxidase 3[more]
AT1G02400.15.5e-8050.16 gibberellin 2-oxidase 6[more]
AT1G47990.12.7e-7449.85 gibberellin 2-oxidase 4[more]
BLAST of CmaCh16G004530 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|32127337|emb|CAC85924.1|2.0e-180100.00Gibberellin 2-oxidase [Cucurbita maxima][more]
gi|27123665|emb|CAC83090.1|4.4e-18099.69gibberellin 2-oxidase [Cucurbita maxima][more]
gi|659101776|ref|XP_008451787.1|4.0e-16590.99PREDICTED: gibberellin 2-beta-dioxygenase 1 [Cucumis melo][more]
gi|700198381|gb|KGN53539.1|1.5e-16490.99hypothetical protein Csa_4G075200 [Cucumis sativus][more]
gi|793421968|ref|NP_001292640.1|3.4e-16490.97gibberellin 2-beta-dioxygenase 1 [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002283Isopenicillin N synthase
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR026992DIOX_N
IPR027443IPNS-like
Vocabulary: Molecular Function
TermDefinition
GO:0005506iron ion binding
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0045487 gibberellin catabolic process
biological_process GO:0009416 response to light stimulus
cellular_component GO:0005575 cellular_component
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0052634 C-19 gibberellin 2-beta-dioxygenase activity
molecular_function GO:0046872 metal ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G004530.1CmaCh16G004530.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002283Isopenicillin N synthasePRINTSPR00682IPNSYNTHASEcoord: 235..261
score: 1.0E-7coord: 199..220
score: 1.0E-7coord: 35..52
score: 1.
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 171..273
score: 1.2
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 169..275
score: 12
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 21..112
score: 7.0
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 12..318
score: 6.9
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 14..318
score: 3.0E
NoneNo IPR availablePANTHERPTHR10209:SF91GIBBERELLIN 2-BETA-DIOXYGENASE 1coord: 14..318
score: 3.0E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 18..315
score: 6.87

Synteny