Carg11378 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg11378
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Gibberellin 2-beta-dioxygenase
Location	Cucurbita_argyrosperma_scaffold_025 : 621321 .. 622587 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CCAGCAATCCAACAGTTGTCTTTTGATTCCTTAATGAGAAGCTCCACGTCCATGGCGGCTGATTCTTCTTCTTCCTCTGCAGCATTCTTCTCTGGGATTCCATTGATAGACCTCTCTGCACCAGATGCTAAACAACTCATTGTCAAAGCTTGTGAAGAACTCGGATTCTTTAAGGTTGTCAACCATGGTGTCCCCATGGAATTCATCTCCTCTCTTGAATCAGAATCCACAAAATTCTTCTCCCTTCCCCTCTCTGAAAAACAGAGAGCTGGCCCTCCTTCCCCTTTTGGCTATGGAAACAAACAAATTGGCTGCAATGGCGATGTCGGTTGGGTTGAATATCTCCTCTTGAACACTCATCTCGAATCCAACTCCGATGGGTTCCTCTCCATTTTTGGCCAAGACCCACAAAAACTCCGGTAACTAAAACACCCAAATCCCCATTTTCCCAGAATGCTCTGTTTTTCACTCTGTTTCTTATACTCTGTTTTTTTTTTTCTCTCTGTTCTCTGTTTCTACGTTTTAGCTCTGCTGTGAACGATTACATCTTGGCTGTGAGGAACATGGCGGGTGAAATCCTCGAGCTAATGGCGGAAGGGTTGATGATTCAACAACGAAACGTGTTCAGTAAGCTCGTGATGGATGAACAGAGCGACTCTGTTTTCAGAGTGAACCATTATCCGCCATGTCCAGACCTTCAAGCTTTAAAAGGAACAAACATGATTGGATTTGGAGAACACACAGACCCTCAAATCATTTCAGTTTTGAGATCAAACAACACTTCTGGATTTCAAATCTCTCTCGCAGATGGGAATTGGATATCTGTTCCTCCCGATCACAGCTCCTTCTTCATCAATGTTGGCGACTCTTTACAGGTACAATAAACACGACAACCTCTGTTCTTCATAATAAAAAAAAGATTCAAAATTTGAGGTTTTCCCCATCTGGGTTCTCCAAAAAAACATCACCCACCATGTTCTTCATTAAAATAATGAAACTTTTTGTGTCGATGAAGGTGATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGGGTTTTGACAAACAGCTCGAAGTCAAGGGTTTCAATGATCTACTTCGGTGGGCCACCGTTGAGTGAAAAGATAGCTCCTTTAGCTTCCCTTATGCAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACATGGTTTGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCTGACAACAGGCTTGTGCCCTTTGAAAGAATTGCAGCCTCATAA

mRNA sequence

CCAGCAATCCAACAGTTGTCTTTTGATTCCTTAATGAGAAGCTCCACGTCCATGGCGGCTGATTCTTCTTCTTCCTCTGCAGCATTCTTCTCTGGGATTCCATTGATAGACCTCTCTGCACCAGATGCTAAACAACTCATTGTCAAAGCTTGTGAAGAACTCGGATTCTTTAAGGTTGTCAACCATGGTGTCCCCATGGAATTCATCTCCTCTCTTGAATCAGAATCCACAAAATTCTTCTCCCTTCCCCTCTCTGAAAAACAGAGAGCTGGCCCTCCTTCCCCTTTTGGCTATGGAAACAAACAAATTGGCTGCAATGGCGATGTCGGTTGGGTTGAATATCTCCTCTTGAACACTCATCTCGAATCCAACTCCGATGGGTTCCTCTCCATTTTTGGCCAAGACCCACAAAAACTCCGCTCTGCTGTGAACGATTACATCTTGGCTGTGAGGAACATGGCGGGTGAAATCCTCGAGCTAATGGCGGAAGGGTTGATGATTCAACAACGAAACGTGTTCAGTAAGCTCGTGATGGATGAACAGAGCGACTCTGTTTTCAGAGTGAACCATTATCCGCCATGTCCAGACCTTCAAGCTTTAAAAGGAACAAACATGATTGGATTTGGAGAACACACAGACCCTCAAATCATTTCAGTTTTGAGATCAAACAACACTTCTGGATTTCAAATCTCTCTCGCAGATGGGAATTGGATATCTGTTCCTCCCGATCACAGCTCCTTCTTCATCAATGTTGGCGACTCTTTACAGGTGATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGGGTTTTGACAAACAGCTCGAAGTCAAGGGTTTCAATGATCTACTTCGGTGGGCCACCGTTGAGTGAAAAGATAGCTCCTTTAGCTTCCCTTATGCAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACATGGTTTGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCTGACAACAGGCTTGTGCCCTTTGAAAGAATTGCAGCCTCATAA

Coding sequence (CDS)

ATGAGAAGCTCCACGTCCATGGCGGCTGATTCTTCTTCTTCCTCTGCAGCATTCTTCTCTGGGATTCCATTGATAGACCTCTCTGCACCAGATGCTAAACAACTCATTGTCAAAGCTTGTGAAGAACTCGGATTCTTTAAGGTTGTCAACCATGGTGTCCCCATGGAATTCATCTCCTCTCTTGAATCAGAATCCACAAAATTCTTCTCCCTTCCCCTCTCTGAAAAACAGAGAGCTGGCCCTCCTTCCCCTTTTGGCTATGGAAACAAACAAATTGGCTGCAATGGCGATGTCGGTTGGGTTGAATATCTCCTCTTGAACACTCATCTCGAATCCAACTCCGATGGGTTCCTCTCCATTTTTGGCCAAGACCCACAAAAACTCCGCTCTGCTGTGAACGATTACATCTTGGCTGTGAGGAACATGGCGGGTGAAATCCTCGAGCTAATGGCGGAAGGGTTGATGATTCAACAACGAAACGTGTTCAGTAAGCTCGTGATGGATGAACAGAGCGACTCTGTTTTCAGAGTGAACCATTATCCGCCATGTCCAGACCTTCAAGCTTTAAAAGGAACAAACATGATTGGATTTGGAGAACACACAGACCCTCAAATCATTTCAGTTTTGAGATCAAACAACACTTCTGGATTTCAAATCTCTCTCGCAGATGGGAATTGGATATCTGTTCCTCCCGATCACAGCTCCTTCTTCATCAATGTTGGCGACTCTTTACAGGTGATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGGGTTTTGACAAACAGCTCGAAGTCAAGGGTTTCAATGATCTACTTCGGTGGGCCACCGTTGAGTGAAAAGATAGCTCCTTTAGCTTCCCTTATGCAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACATGGTTTGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCTGACAACAGGCTTGTGCCCTTTGAAAGAATTGCAGCCTCATAA

Protein sequence

MRSSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRLVPFERIAAS

Homology

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BLAST of Carg11378 vs. NCBI nr
Match: XP_023551922.1 (gibberellin 2-beta-dioxygenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 644.4 bits (1661), Expect = 2.0e-181
Identity = 324/328 (98.78%), Postives = 325/328 (99.09%), Query Frame = 0


Query: 1   MRSSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS 60
           MRSSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS
Sbjct: 1   MRSSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS 60

Query: 61  LESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSI 120
           LE ESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSI
Sbjct: 61  LELESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSI 120

Query: 121 FGQDPQKLRSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHY 180
           FGQDPQKLRSAVNDYI AVRNMAGEILELMAEGLMIQQRNVFSKLVMDE+SDSVFRVNHY
Sbjct: 121 FGQDPQKLRSAVNDYISAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHY 180

Query: 181 PPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240
           PPCPDLQALKG NMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV
Sbjct: 181 PPCPDLQALKGRNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240

Query: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300
           GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF
Sbjct: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300

Query: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 329
           TWFEYKRSAYNSRLADNRLVPFERIAAS
Sbjct: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 328

[Close]

BLAST of Carg11378 vs. NCBI nr
Match: XP_022985064.1 (gibberellin 2-beta-dioxygenase 1-like [Cucurbita maxima])

HSP 1 Score: 627.1 bits (1616), Expect = 3.4e-176
Identity = 319/328 (97.26%), Postives = 321/328 (97.87%), Query Frame = 0


Query: 1   MRSSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS 60
           MRSSTSMAA +SS SAAF+SGIPLIDLSAPDAKQLIVKACEELGFFKVV HGVPME ISS
Sbjct: 1   MRSSTSMAA-ASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISS 60

Query: 61  LESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSI 120
           LESESTKFFSLPLSEKQRAGPPSPFGYGNKQIG NGDVGWVEYLLLNTHLESNSDGFLSI
Sbjct: 61  LESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSI 120

Query: 121 FGQDPQKLRSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHY 180
           FGQDPQKLRSAVNDYI AVRNMAGEILELMAEGL IQQRNVFSKLVMDEQSDSVFRVNHY
Sbjct: 121 FGQDPQKLRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHY 180

Query: 181 PPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240
           PPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV
Sbjct: 181 PPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240

Query: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300
           GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF
Sbjct: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300

Query: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 329
           TWFEYKRSAYNSRLADNRLVPFERIAAS
Sbjct: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 327

[Close]

BLAST of Carg11378 vs. NCBI nr
Match: XP_022931327.1 (gibberellin 2-beta-dioxygenase 1-like [Cucurbita moschata])

HSP 1 Score: 619.0 bits (1595), Expect = 9.2e-174
Identity = 308/309 (99.68%), Postives = 308/309 (99.68%), Query Frame = 0


Query: 20  SGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEKQRA 79
           SGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEKQRA
Sbjct: 107 SGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEKQRA 166

Query: 80  GPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYILAV 139
           GPPSPFGYGNKQIG NGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYILAV
Sbjct: 167 GPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYILAV 226

Query: 140 RNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFGE 199
           RNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFGE
Sbjct: 227 RNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFGE 286

Query: 200 HTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRV 259
           HTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRV
Sbjct: 287 HTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRV 346

Query: 260 LTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRL 319
           LTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRL
Sbjct: 347 LTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRL 406

Query: 320 VPFERIAAS 329
           VPFERIAAS
Sbjct: 407 VPFERIAAS 415

[Close]

BLAST of Carg11378 vs. NCBI nr
Match: CAC85924.1 (Gibberellin 2-oxidase [Cucurbita maxima])

HSP 1 Score: 618.6 bits (1594), Expect = 1.2e-173
Identity = 311/320 (97.19%), Postives = 313/320 (97.81%), Query Frame = 0


Query: 9   ADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKF 68
           A +SS SAAF+SGIPLIDLSAPDAKQLIVKACEELGFFKVV HGVPME ISSLESESTKF
Sbjct: 2   AAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKF 61

Query: 69  FSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 128
           FSLPLSEKQRAGPPSPFGYGNKQIG NGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL
Sbjct: 62  FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 121

Query: 129 RSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 188
           RSAVNDYI AVRNMAGEILELMAEGL IQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA
Sbjct: 122 RSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 181

Query: 189 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 248
           LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT
Sbjct: 182 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 241

Query: 249 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 308
           NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS
Sbjct: 242 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 301

Query: 309 AYNSRLADNRLVPFERIAAS 329
           AYNSRLADNRLVPFERIAAS
Sbjct: 302 AYNSRLADNRLVPFERIAAS 321

[Close]

BLAST of Carg11378 vs. NCBI nr
Match: CAC83090.1 (gibberellin 2-oxidase [Cucurbita maxima])

HSP 1 Score: 617.5 bits (1591), Expect = 2.7e-173
Identity = 310/320 (96.88%), Postives = 313/320 (97.81%), Query Frame = 0


Query: 9   ADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKF 68
           A +SS SAAF+SGIPLIDLSAPDAKQLIVKACEELGFFKVV HGVPME ISSLESESTKF
Sbjct: 2   AAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKF 61

Query: 69  FSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 128
           FSLPLSEKQRAGPPSPFGYGNKQIG NGDVGWVEYLLLNTHLESNSDGFLS+FGQDPQKL
Sbjct: 62  FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSMFGQDPQKL 121

Query: 129 RSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 188
           RSAVNDYI AVRNMAGEILELMAEGL IQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA
Sbjct: 122 RSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 181

Query: 189 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 248
           LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT
Sbjct: 182 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 241

Query: 249 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 308
           NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS
Sbjct: 242 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 301

Query: 309 AYNSRLADNRLVPFERIAAS 329
           AYNSRLADNRLVPFERIAAS
Sbjct: 302 AYNSRLADNRLVPFERIAAS 321

[Close]

BLAST of Carg11378 vs. TAIR10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1)

HSP 1 Score: 387.1 bits (993), Expect = 1.1e-107
Identity = 192/309 (62.14%), Postives = 233/309 (75.40%), Query Frame = 0


Query: 19  FSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEK-Q 78
           FS IP+ID+S P++K  +VKACE+ GFFKV+NHGV  E +S LE E+  FFSLP SEK Q
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 79  RAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYIL 138
            AG   PFGYGN +IG NGDVGWVEYLL+N + +S S        + P   R+A+ +Y  
Sbjct: 75  VAG--YPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTT 134

Query: 139 AVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCP--DLQALKGTNMI 198
           +VR M  ++LE + +GL I+ RN  SKLV D+ +DS+ R+NHYPPCP  + +   G N+I
Sbjct: 135 SVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVI 194

Query: 199 GFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSV 258
           GFGEHTDPQIISVLRSNNTSG QI+L DG+WISVPPDH+SFF NVGDSLQVMTNGRFKSV
Sbjct: 195 GFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSV 254

Query: 259 KHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLA 318
           +HRVL N  KSRVSMIYF GP L+++IAPL  L+  E+  LY+EFTW EYK S YNSRL+
Sbjct: 255 RHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLS 314

Query: 319 DNRLVPFER 325
           DNRL  FER
Sbjct: 315 DNRLQQFER 321

[Close]

BLAST of Carg11378 vs. TAIR10
Match: AT1G30040.1 (gibberellin 2-oxidase)

HSP 1 Score: 356.7 bits (914), Expect = 1.5e-98
Identity = 177/303 (58.42%), Postives = 224/303 (73.93%), Query Frame = 0


Query: 22  IPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEKQRAGP 81
           IP+++L+ P+AK  IVKACEE GFFKVVNHGV  E ++ LE E+  FF LP S K RAGP
Sbjct: 31  IPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGP 90

Query: 82  PSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYILAVRN 141
           P P+GYGNK+IG NGDVGW+EYLLLN + + +S    ++F Q P                
Sbjct: 91  PEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPXXXXXXXXXXXXXXXX 150

Query: 142 MAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFGEHT 201
            + ++LE++AE L I+ R+  SK++ DE+SDS  R+NHYP   + +A K    +GFGEHT
Sbjct: 151 XSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEE-EAEKMVK-VGFGEHT 210

Query: 202 DPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRVLT 261
           DPQIISVLRSNNT+G QI + DG+W++VPPDHSSFFINVGD+LQVMTNGRFKSVKHRVL 
Sbjct: 211 DPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVKHRVLA 270

Query: 262 NSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRLVP 321
           ++ +SR+SMIYFGGPPLS+KIAPL  L+  ++  LYKEFTW +YK SAY S+L D RL  
Sbjct: 271 DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGL 330

Query: 322 FER 325
           FE+
Sbjct: 331 FEK 331

[Close]

BLAST of Carg11378 vs. TAIR10
Match: AT2G34555.1 (gibberellin 2-oxidase 3)

HSP 1 Score: 345.1 bits (884), Expect = 4.7e-95
Identity = 169/303 (55.78%), Postives = 218/303 (71.95%), Query Frame = 0


Query: 22  IPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEKQRAGP 81
           IP+IDL+  DAK  IVKACEE GFFKV+NHGV  + ++ LE E+  FF+L  S K +AGP
Sbjct: 27  IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGP 86

Query: 82  PSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYILAVRN 141
           P PFGYG K+IG NGD+GW+EY+LLN +L   S    +IF   P   R AV +YI  ++ 
Sbjct: 87  PDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKR 146

Query: 142 MAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFGEHT 201
           M+ + LE++ E L I+ +   S+LV  ++SDS  R+NHY   P+ +       IGFGEHT
Sbjct: 147 MSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHY---PEKEETPVKEEIGFGEHT 206

Query: 202 DPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRVLT 261
           DPQ+IS+LRSN+T G QI + DG W+ V PDHSSFF+ VGD+LQVMTNGRFKSVKHRV+T
Sbjct: 207 DPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVT 266

Query: 262 NSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRLVP 321
           N+ +SR+SMIYF GPPLSEKIAPL+ L+  ++  LY EFTW +YK SAY ++L D RL  
Sbjct: 267 NTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGL 326

Query: 322 FER 325
           FE+
Sbjct: 327 FEK 326

[Close]

BLAST of Carg11378 vs. TAIR10
Match: AT1G02400.1 (gibberellin 2-oxidase 6)

HSP 1 Score: 294.3 bits (752), Expect = 9.4e-80
Identity = 158/327 (48.32%), Postives = 209/327 (63.91%), Query Frame = 0


Query: 3   SSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQL---IVKACEELGFFKVVNHGVPMEFIS 62
           SST +      + ++     P+ID S  D  +L   IVKACE  GFFKV+NHGV  E I 
Sbjct: 5   SSTPLQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIK 64

Query: 63  SLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLS 122
             E E  +FF+ P S+K RAGP SPFGYG K IG NGD+G +EYLLL+ +  + +D   +
Sbjct: 65  RFEHEGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSET 124

Query: 123 IFGQDPQKLRSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNH 182
           I   DP K  SA NDYI  VR++A EI++L  E L  Q+ +  S+L+ D +SDS+ R+NH
Sbjct: 125 ISHDDPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNH 184

Query: 183 YPPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFIN 242
           YPP P   AL G   IGFGEH+DPQI++VLRSN+  G +I   DG WI +P D + FF+ 
Sbjct: 185 YPPAP--YALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVL 244

Query: 243 VGDSLQVMTNGRFKSVKHRVLTNSSKS-RVSMIYFGGPPLSEKIAPLASLMQGEERSLYK 302
           VGD LQ +TNGRF SV+HRVL N++K  R+S +YF  PPL  KI+PL  ++  E    Y 
Sbjct: 245 VGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYN 304

Query: 303 EFTWFEYKRSAYNSRLADNRLVPFERI 326
            FTW +YK++ Y+ RL   RL  F+ +
Sbjct: 305 SFTWGDYKKATYSLRLDVPRLEFFKTL 329

[Close]

BLAST of Carg11378 vs. TAIR10
Match: AT1G47990.1 (gibberellin 2-oxidase 4)

HSP 1 Score: 276.9 bits (707), Expect = 1.6e-74
Identity = 163/323 (50.46%), Postives = 198/323 (61.30%), Query Frame = 0


Query: 12  SSSSAAFFSGIPLIDLS--APDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFF 71
           S    A    IP+ID+S         IVKACE LGFFKV+NHGV    IS +E ES  FF
Sbjct: 5   SQKIVAVDQDIPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFF 64

Query: 72  SLPLSEKQRAGPPS-PFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 131
           + P  EK+   P + PF YG + IG NGD G VEYLL +T    N   F S         
Sbjct: 65  AKPAHEKKSVRPVNQPFRYGFRDIGLNGDSGEVEYLLFHT----NDPAFRS-----QLSF 124

Query: 132 RSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDL-- 191
            SAVN YI AV+ +A EIL+L AEGL +   + FS+L+    SDSV RVNHYPP      
Sbjct: 125 SSAVNCYIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFG 184

Query: 192 ------QALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 251
                 Q++  T  +GFGEHTDPQI++VLRSN   G Q+S +DG W+SV PD S+F +NV
Sbjct: 185 EANLSDQSVSLTR-VGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVNV 244

Query: 252 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPL-ASLMQGEERSLYKE 311
           GD LQVMTNGRF SV+HR LT   +SR+S  YF GPPL  KI PL A +M   +  LY+ 
Sbjct: 245 GDLLQVMTNGRFISVRHRALTYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLYQT 304

Query: 312 FTWFEYKRSAYNSRLADNRLVPF 323
           FTW EYK+ AY+ RL D+RL  F
Sbjct: 305 FTWGEYKKRAYSLRLEDSRLDMF 316

[Close]

BLAST of Carg11378 vs. Swiss-Prot
Match: sp|Q9SQ80|G2OX1_PEA (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 1.8e-128
Identity = 227/311 (72.99%), Postives = 261/311 (83.92%), Query Frame = 0


Query: 18  FFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEKQ 77
           F S IPL+DLS PDAK LIVKACE+ GFFKV+NHG+P++ IS LESE+ KFFSLP +EK+
Sbjct: 23  FSSSIPLVDLSKPDAKTLIVKACEDFGFFKVINHGIPLDAISQLESEAFKFFSLPQTEKE 82

Query: 78  RAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYIL 137
           +AGP +PFGYGNK+IG NGD+GW+EYLLL T    N D   S++G+D  K R  + DY  
Sbjct: 83  KAGPANPFGYGNKRIGLNGDIGWIEYLLLTT----NQDHNFSLYGEDIDKFRGLLKDYKC 142

Query: 138 AVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGF 197
           A+RNMA EIL+LMAEGL IQ +NVFSKLVMD+QSD +FRVNHYP CP+L A+ G N+IGF
Sbjct: 143 AMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLFRVNHYPACPEL-AINGENLIGF 202

Query: 198 GEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKH 257
           GEHTDPQIIS+LRSNNTSGFQISL DG+WISVPPDHSSFFINVGDSLQVMTNGRFKSV+H
Sbjct: 203 GEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSSFFINVGDSLQVMTNGRFKSVRH 262

Query: 258 RVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADN 317
           RVL N    R+SMIYF GPPLSEKIAPL SLM+G+E SLYKEFTWFEYK S Y SRLADN
Sbjct: 263 RVLANGIDPRLSMIYFCGPPLSEKIAPLPSLMKGKE-SLYKEFTWFEYKSSTYGSRLADN 322

Query: 318 RLVPFERIAAS 329
           RL  +ERIAA+
Sbjct: 323 RLGNYERIAAT 327

[Close]

BLAST of Carg11378 vs. Swiss-Prot
Match: sp|Q9XG83|G2OX_PHACN (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 5.2e-120
Identity = 208/313 (66.45%), Postives = 253/313 (80.83%), Query Frame = 0


Query: 15  SAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLS 74
           S   F+GIP++DL+ PDAK LIV AC + GFFK+VNHGVP+E +++LE+E+ +FF    S
Sbjct: 20  STPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHGVPLELMANLENEALRFFKKSQS 79

Query: 75  EKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVND 134
           EK RAGPP PFGYG+K+IG NGDVGWVEYLLLNT+ +  S   L IF ++P   R+ V +
Sbjct: 80  EKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVISPKSLCIFRENPHHFRAVVEN 139

Query: 135 YILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNM 194
           YI AV+NM   +LELMAEGL I+QRN  S+L+ DE+SDS FR+NHYPPCP++QAL   N+
Sbjct: 140 YITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCFRLNHYPPCPEVQAL-NRNL 199

Query: 195 IGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKS 254
           +GFGEHTDPQIISVLRSN+TSG QI L DG W+SVPPD +SFFINVGD+LQVMTNGRFKS
Sbjct: 200 VGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTSFFINVGDALQVMTNGRFKS 259

Query: 255 VKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRL 314
           VKHRVL +++KSR+SMIYFGGP LSE IAPL S+M   E  LYKEFTW EYK++AY SRL
Sbjct: 260 VKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVMLKGEECLYKEFTWCEYKKAAYTSRL 319

Query: 315 ADNRLVPFERIAA 328
           ADNRL PF++ AA
Sbjct: 320 ADNRLAPFQKSAA 331

[Close]

BLAST of Carg11378 vs. Swiss-Prot
Match: sp|Q8LEA2|G2OX1_ARATH (Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 SV=2)

HSP 1 Score: 387.1 bits (993), Expect = 1.9e-106
Identity = 192/309 (62.14%), Postives = 233/309 (75.40%), Query Frame = 0


Query: 19  FSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEK-Q 78
           FS IP+ID+S P++K  +VKACE+ GFFKV+NHGV  E +S LE E+  FFSLP SEK Q
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 79  RAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYIL 138
            AG   PFGYGN +IG NGDVGWVEYLL+N + +S S        + P   R+A+ +Y  
Sbjct: 75  VAG--YPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTT 134

Query: 139 AVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCP--DLQALKGTNMI 198
           +VR M  ++LE + +GL I+ RN  SKLV D+ +DS+ R+NHYPPCP  + +   G N+I
Sbjct: 135 SVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVI 194

Query: 199 GFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSV 258
           GFGEHTDPQIISVLRSNNTSG QI+L DG+WISVPPDH+SFF NVGDSLQVMTNGRFKSV
Sbjct: 195 GFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSV 254

Query: 259 KHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLA 318
           +HRVL N  KSRVSMIYF GP L+++IAPL  L+  E+  LY+EFTW EYK S YNSRL+
Sbjct: 255 RHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLS 314

Query: 319 DNRLVPFER 325
           DNRL  FER
Sbjct: 315 DNRLQQFER 321

[Close]

BLAST of Carg11378 vs. Swiss-Prot
Match: sp|Q8S0S6|G2OX3_ORYSJ (Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX3 PE=1 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 6.0e-100
Identity = 181/306 (59.15%), Postives = 224/306 (73.20%), Query Frame = 0


Query: 19  FSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEKQR 78
           FSG+P++DL +P A + +V ACE  GFFKVVNHGV  + +   ESE+ +FFS    +K R
Sbjct: 25  FSGVPVVDLGSPGAARAVVDACERYGFFKVVNHGVATDTMDKAESEAVRFFSQTQPDKDR 84

Query: 79  AGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYILA 138
           +GP  PFGYG+K+IG NGD+GW+EYLLL     S +D   +         R+A+N+YI  
Sbjct: 85  SGPAYPFGYGSKRIGFNGDMGWLEYLLLALDDASLAD---ACTVPSCAVFRAALNEYISG 144

Query: 139 VRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFG 198
           VR +A  ++E M+EGL I Q +  S LV  E SD VFRVNHYPPC  LQ L G ++ GFG
Sbjct: 145 VRKVAVRVMEAMSEGLGIAQADALSALVTAEGSDQVFRVNHYPPCRALQGL-GCSVTGFG 204

Query: 199 EHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHR 258
           EHTDPQ++SVLRSN TSG QI+L DG W+SVP D  SFF+NVGDSLQV+TNGRFKSVKHR
Sbjct: 205 EHTDPQLVSVLRSNGTSGLQIALRDGQWVSVPSDRDSFFVNVGDSLQVLTNGRFKSVKHR 264

Query: 259 VLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNR 318
           V+ NS KSRVS IYFGGPPL+++IAPL  L+   E+SLYKEFTW EYK++AY SRL DNR
Sbjct: 265 VVANSLKSRVSFIYFGGPPLAQRIAPLPQLLGEGEQSLYKEFTWDEYKKAAYKSRLGDNR 324

Query: 319 LVPFER 325
           L  FE+
Sbjct: 325 LAQFEK 326

[Close]

BLAST of Carg11378 vs. Swiss-Prot
Match: sp|Q9XFR9|G2OX2_ARATH (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 2.8e-97
Identity = 177/303 (58.42%), Postives = 224/303 (73.93%), Query Frame = 0


Query: 22  IPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKFFSLPLSEKQRAGP 81
           IP+++L+ P+AK  IVKACEE GFFKVVNHGV  E ++ LE E+  FF LP S K RAGP
Sbjct: 31  IPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGP 90

Query: 82  PSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYILAVRN 141
           P P+GYGNK+IG NGDVGW+EYLLLN + + +S    ++F Q P                
Sbjct: 91  PEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPXXXXXXXXXXXXXXXX 150

Query: 142 MAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQALKGTNMIGFGEHT 201
            + ++LE++AE L I+ R+  SK++ DE+SDS  R+NHYP   + +A K    +GFGEHT
Sbjct: 151 XSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEE-EAEKMVK-VGFGEHT 210

Query: 202 DPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRVLT 261
           DPQIISVLRSNNT+G QI + DG+W++VPPDHSSFFINVGD+LQVMTNGRFKSVKHRVL 
Sbjct: 211 DPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVKHRVLA 270

Query: 262 NSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRLVP 321
           ++ +SR+SMIYFGGPPLS+KIAPL  L+  ++  LYKEFTW +YK SAY S+L D RL  
Sbjct: 271 DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGL 330

Query: 322 FER 325
           FE+
Sbjct: 331 FEK 331

[Close]

BLAST of Carg11378 vs. TrEMBL
Match: tr|Q7X9N5|Q7X9N5_CUCMA (Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox PE=2 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 8.0e-174
Identity = 311/320 (97.19%), Postives = 313/320 (97.81%), Query Frame = 0


Query: 9   ADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKF 68
           A +SS SAAF+SGIPLIDLSAPDAKQLIVKACEELGFFKVV HGVPME ISSLESESTKF
Sbjct: 2   AAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKF 61

Query: 69  FSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 128
           FSLPLSEKQRAGPPSPFGYGNKQIG NGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL
Sbjct: 62  FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 121

Query: 129 RSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 188
           RSAVNDYI AVRNMAGEILELMAEGL IQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA
Sbjct: 122 RSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 181

Query: 189 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 248
           LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT
Sbjct: 182 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 241

Query: 249 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 308
           NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS
Sbjct: 242 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 301

Query: 309 AYNSRLADNRLVPFERIAAS 329
           AYNSRLADNRLVPFERIAAS
Sbjct: 302 AYNSRLADNRLVPFERIAAS 321

[Close]

BLAST of Carg11378 vs. TrEMBL
Match: tr|Q8GUE8|Q8GUE8_CUCMA (Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox PE=2 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 1.8e-173
Identity = 310/320 (96.88%), Postives = 313/320 (97.81%), Query Frame = 0


Query: 9   ADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKF 68
           A +SS SAAF+SGIPLIDLSAPDAKQLIVKACEELGFFKVV HGVPME ISSLESESTKF
Sbjct: 2   AAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKF 61

Query: 69  FSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 128
           FSLPLSEKQRAGPPSPFGYGNKQIG NGDVGWVEYLLLNTHLESNSDGFLS+FGQDPQKL
Sbjct: 62  FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSMFGQDPQKL 121

Query: 129 RSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 188
           RSAVNDYI AVRNMAGEILELMAEGL IQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA
Sbjct: 122 RSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 181

Query: 189 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 248
           LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT
Sbjct: 182 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 241

Query: 249 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 308
           NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS
Sbjct: 242 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 301

Query: 309 AYNSRLADNRLVPFERIAAS 329
           AYNSRLADNRLVPFERIAAS
Sbjct: 302 AYNSRLADNRLVPFERIAAS 321

[Close]

BLAST of Carg11378 vs. TrEMBL
Match: tr|A0A1S3BRQ7|A0A1S3BRQ7_CUCME (gibberellin 2-beta-dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103492969 PE=3 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 9.8e-164
Identity = 295/328 (89.94%), Postives = 308/328 (93.90%), Query Frame = 0


Query: 1   MRSSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS 60
           MR+S + AA   SSSAAFFS +PLIDLS+PDAKQLIVKACEELGFFKVVNHGVPMEFIS+
Sbjct: 18  MRNSMASAA---SSSAAFFSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFIST 77

Query: 61  LESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSI 120
           LESEST FFSLPLSEKQ+A PPSPFGYGNKQIG NGDVGWVEY+LLNTHLESNSDGFLSI
Sbjct: 78  LESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSI 137

Query: 121 FGQDPQKLRSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHY 180
           FGQDPQKLRSAVN+YI AVRNMA EILELMAEGL IQQRN FSKLVMDE+SDSVFRVNHY
Sbjct: 138 FGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNAFSKLVMDEESDSVFRVNHY 197

Query: 181 PPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240
           PPCP +QALKG NMIGFGEHTDPQIISVLRSNNTSG QISLADGNWISVPPD +SFFINV
Sbjct: 198 PPCPQIQALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINV 257

Query: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300
           GDSLQVMTNGRFKSVKHRVLTNS KSR+SMIYFGGPPLSEKIAPL SLM+GEERSLYKEF
Sbjct: 258 GDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEF 317

Query: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 329
           TWFEYKRSAYNSRLADNRLV FERIAAS
Sbjct: 318 TWFEYKRSAYNSRLADNRLVHFERIAAS 342

[Close]

BLAST of Carg11378 vs. TrEMBL
Match: tr|A0A0A0KXI6|A0A0A0KXI6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G075200 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 6.3e-163
Identity = 294/328 (89.63%), Postives = 307/328 (93.60%), Query Frame = 0


Query: 1   MRSSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS 60
           MR+S + AA   SSS AF S +PLIDLS+PDAKQLIVKACEELGFFKVVNHGVPMEFIS+
Sbjct: 18  MRNSMASAA---SSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFIST 77

Query: 61  LESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSI 120
           LESEST FFSLPLSEKQ+A PPSPFGYGNKQIG NGDVGWVEY+LLNTHLESNSDGFLSI
Sbjct: 78  LESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSI 137

Query: 121 FGQDPQKLRSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHY 180
           FGQDPQKLRSAVN+YI AVRNMA EILELMAEGL IQQRNVFSKLVMDE+SDSVFRVNHY
Sbjct: 138 FGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHY 197

Query: 181 PPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240
           PPCP +QALKG NMIGFGEHTDPQIISVLRSNNTSG QISLADGNWISVPPD +SFFINV
Sbjct: 198 PPCPQIQALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINV 257

Query: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300
           GDSLQVMTNGRFKSVKHRVLTNS KSR+SMIYFGGPPLSEKIAPL SLM+GEERSLYKEF
Sbjct: 258 GDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEF 317

Query: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 329
           TWFEYKRSAYNSRLADNRLV FERIAAS
Sbjct: 318 TWFEYKRSAYNSRLADNRLVHFERIAAS 342

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BLAST of Carg11378 vs. TrEMBL
Match: tr|S0BDZ9|S0BDZ9_CUCSA (GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox1 PE=2 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 8.3e-163
Identity = 290/320 (90.62%), Postives = 303/320 (94.69%), Query Frame = 0


Query: 9   ADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLESESTKF 68
           A ++SSS AF S +PLIDLS+PDAKQLIVKACEELGFFKVVNHGVPMEFIS+LESEST F
Sbjct: 2   ASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNF 61

Query: 69  FSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 128
           FSLPLSEKQ+A PPSPFGYGNKQIG NGDVGWVEY+LLNTHLESNSDGFLSIFGQDPQKL
Sbjct: 62  FSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQKL 121

Query: 129 RSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 188
           RSAVN+YI AVRNMA EILELMAEGL IQQRNVFSKLVMDE+SDSVFRVNHYPPCP +QA
Sbjct: 122 RSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQA 181

Query: 189 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 248
           LKG NMIGFGEHTDPQIISVLRSNNTSG QISLADGNWISVPPD +SFFINVGDSLQVMT
Sbjct: 182 LKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMT 241

Query: 249 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 308
           NGRFKSVKHRVLTNS KSR+SMIYFGGPPLSEKIAPL SLM+GEERSLYKEFTWFEYKRS
Sbjct: 242 NGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRS 301

Query: 309 AYNSRLADNRLVPFERIAAS 329
           AYNSRLADNRLV FERIAAS
Sbjct: 302 AYNSRLADNRLVHFERIAAS 321

The following BLAST results are available for this feature:

BLAST of Carg11378 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023551922.1	2.0e-181	98.78	gibberellin 2-beta-dioxygenase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022985064.1	3.4e-176	97.26	gibberellin 2-beta-dioxygenase 1-like [Cucurbita maxima]	[more]
XP_022931327.1	9.2e-174	99.68	gibberellin 2-beta-dioxygenase 1-like [Cucurbita moschata]	[more]
CAC85924.1	1.2e-173	97.19	Gibberellin 2-oxidase [Cucurbita maxima]	[more]
CAC83090.1	2.7e-173	96.88	gibberellin 2-oxidase [Cucurbita maxima]	[more]

BLAST of Carg11378 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G78440.1	1.1e-107	62.14	Arabidopsis thaliana gibberellin 2-oxidase 1	[more]
AT1G30040.1	1.5e-98	58.42	gibberellin 2-oxidase	[more]
AT2G34555.1	4.7e-95	55.78	gibberellin 2-oxidase 3	[more]
AT1G02400.1	9.4e-80	48.32	gibberellin 2-oxidase 6	[more]
AT1G47990.1	1.6e-74	50.46	gibberellin 2-oxidase 4	[more]

BLAST of Carg11378 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9SQ80\|G2OX1_PEA	1.8e-128	72.99	Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1	[more]
sp\|Q9XG83\|G2OX_PHACN	5.2e-120	66.45	Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=...	[more]
sp\|Q8LEA2\|G2OX1_ARATH	1.9e-106	62.14	Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 ...	[more]
sp\|Q8S0S6\|G2OX3_ORYSJ	6.0e-100	59.15	Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
sp\|Q9XFR9\|G2OX2_ARATH	2.8e-97	58.42	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]

BLAST of Carg11378 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|Q7X9N5\|Q7X9N5_CUCMA	8.0e-174	97.19	Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox PE=2 SV=1	[more]
tr\|Q8GUE8\|Q8GUE8_CUCMA	1.8e-173	96.88	Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox PE=2 SV=1	[more]
tr\|A0A1S3BRQ7\|A0A1S3BRQ7_CUCME	9.8e-164	89.94	gibberellin 2-beta-dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103492969 PE=3 SV...	[more]
tr\|A0A0A0KXI6\|A0A0A0KXI6_CUCSA	6.3e-163	89.63	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G075200 PE=3 SV=1	[more]
tr\|S0BDZ9\|S0BDZ9_CUCSA	8.3e-163	90.63	GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox1 PE=2 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR027443	IPNS-like
IPR026992	DIOX_N
IPR005123	Oxoglu/Fe-dep_dioxygenase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg11378-RA	Carg11378-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 36..53 score: 54.55 coord: 200..221 score: 27.69 coord: 236..262 score: 33.0
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 13..324
None	No IPR available	PANTHER	PTHR10209:SF380	SUBFAMILY NOT NAMED	coord: 13..324
None	No IPR available	SUPERFAMILY	SSF51197	Clavaminate synthase-like	coord: 19..316
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 172..274 e-value: 5.6E-28 score: 97.3
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 170..276 score: 12.386
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 22..113 e-value: 8.3E-16 score: 58.7
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 2..316 e-value: 1.6E-80 score: 272.9

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg11378	Cla005397	Watermelon (97103) v1	carwmB0652
Carg11378	Csa4G075200	Cucumber (Chinese Long) v2	carcuB0676
Carg11378	MELO3C016152	Melon (DHL92) v3.5.1	carmeB0652
Carg11378	ClCG07G010460	Watermelon (Charleston Gray)	carwcgB0637
Carg11378	CSPI04G07580	Wild cucumber (PI 183967)	carcpiB0686
Carg11378	CmaCh16G004530	Cucurbita maxima (Rimu)	carcmaB0924
Carg11378	CmaCh04G006490	Cucurbita maxima (Rimu)	carcmaB0927
Carg11378	CmoCh16G004890	Cucurbita moschata (Rifu)	carcmoB0898
Carg11378	CmoCh04G006990	Cucurbita moschata (Rifu)	carcmoB0902
Carg11378	Lsi07G008540	Bottle gourd (USVL1VR-Ls)	carlsiB596
Carg11378	Cp4.1LG01g00820	Cucurbita pepo (Zucchini)	carcpeB0929
Carg11378	Cp4.1LG14g03420	Cucurbita pepo (Zucchini)	carcpeB0926
Carg11378	CsGy4G007490	Cucumber (Gy14) v2	carcgybB0651
Carg11378	MELO3C016152.2	Melon (DHL92) v3.6.1	carmedB0662
Carg11378	CsaV3_4G007790	Cucumber (Chinese Long) v3	carcucB0688
Carg11378	Cla97C07G137160	Watermelon (97103) v2	carwmbB0682
Carg11378	Bhi01G002439	Wax gourd	carwgoB0770

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg11378	Carg13102	Silver-seed gourd	carcarB050

The following block(s) are covering this gene:


Gene	Organism	Block
Carg11378	Cucumber (Gy14) v2	carcgybB0652
Carg11378	Wild cucumber (PI 183967)	carcpiB0687
Carg11378	Cucumber (Chinese Long) v2	carcuB0677
Carg11378	Cucumber (Chinese Long) v3	carcucB0689