Lsi05G006260 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G006260
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	2S albumin
Location	chr05 : 7706390 .. 7706815 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAGACTCACAAGTATCATTGCCTTCTTAGCGGTGGCTTTGCTGGTTGCAGATGCCTATGCCCACCGCACCACCATCACCACCGTCGAAGTGGACGAAGACAACCAACGGCGGCAGGAGAGGTGCCGCCAGATGAGGGCCCGCGAGGAGATCGGCAGCTGCGCTCAGTACCTGTCACAGCAGAGCAGGTATGTTTTGCAGATGCGTGGAATCGACAACCAACGGAGGAGAGAAGGCCATGCCTTTGAAGAGTGCTGCAATGAGCTGAGGAATGTGGATGAGGAATGCAGGTGCGAACTCCTAGAGGAGATTGCTATGGAAGAGCAGAGGAGAGCACGAGGACAAGAAGGAAGGCAGATGCTTCAGAGAGCTAGAAACTTGCCATCCATGTGTGGAATACGCCCACAGCAATGCTACTTCTAA

mRNA sequence

ATGGCGAGACTCACAAGTATCATTGCCTTCTTAGCGGTGGCTTTGCTGGTTGCAGATGCCTATGCCCACCGCACCACCATCACCACCGTCGAAGTGGACGAAGACAACCAACGGCGGCAGGAGAGGTGCCGCCAGATGAGGGCCCGCGAGGAGATCGGCAGCTGCGCTCAGTACCTGTCACAGCAGAGCAGGTATGTTTTGCAGATGCGTGGAATCGACAACCAACGGAGGAGAGAAGGCCATGCCTTTGAAGAGTGCTGCAATGAGCTGAGGAATGTGGATGAGGAATGCAGGTGCGAACTCCTAGAGGAGATTGCTATGGAAGAGCAGAGGAGAGCACGAGGACAAGAAGGAAGGCAGATGCTTCAGAGAGCTAGAAACTTGCCATCCATGTGTGGAATACGCCCACAGCAATGCTACTTCTAA

Coding sequence (CDS)

ATGGCGAGACTCACAAGTATCATTGCCTTCTTAGCGGTGGCTTTGCTGGTTGCAGATGCCTATGCCCACCGCACCACCATCACCACCGTCGAAGTGGACGAAGACAACCAACGGCGGCAGGAGAGGTGCCGCCAGATGAGGGCCCGCGAGGAGATCGGCAGCTGCGCTCAGTACCTGTCACAGCAGAGCAGGTATGTTTTGCAGATGCGTGGAATCGACAACCAACGGAGGAGAGAAGGCCATGCCTTTGAAGAGTGCTGCAATGAGCTGAGGAATGTGGATGAGGAATGCAGGTGCGAACTCCTAGAGGAGATTGCTATGGAAGAGCAGAGGAGAGCACGAGGACAAGAAGGAAGGCAGATGCTTCAGAGAGCTAGAAACTTGCCATCCATGTGTGGAATACGCCCACAGCAATGCTACTTCTAA

Protein sequence

MARLTSIIAFLAVALLVADAYAHRTTITTVEVDEDNQRRQERCRQMRAREEIGSCAQYLSQQSRYVLQMRGIDNQRRREGHAFEECCNELRNVDEECRCELLEEIAMEEQRRARGQEGRQMLQRARNLPSMCGIRPQQCYF

Homology

[Close]

BLAST of Lsi05G006260 vs. Swiss-Prot
Match: 2SS_CUCMA (2S albumin OS=Cucurbita maxima PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 1.6e-54
Identity = 112/141 (79.43%), Postives = 126/141 (89.36%), Query Frame = 1


Query: 1   MARLTSIIAFLAVALLVADAYAHRTTITTVEVDEDNQRRQERCRQMRAREEIGSCAQYLS 60
           MARLTSIIA  AVALLVADAYA+RTTITTVEV+E+ Q R+ERCRQM AREE+ SC QYL 
Sbjct: 1   MARLTSIIALFAVALLVADAYAYRTTITTVEVEENRQGREERCRQMSAREELRSCEQYLR 60

Query: 61  QQSRYVLQMRGIDNQRRREGHAFEECCNELRNVDEECRCELLEEIAMEEQRRARGQEGRQ 120
           QQSR VLQMRGI+N  RREG +F+ECC EL+NVDEECRC++LEEIA EEQR+ARGQEGRQ
Sbjct: 61  QQSRDVLQMRGIENPWRREGGSFDECCRELKNVDEECRCDMLEEIAREEQRQARGQEGRQ 120

Query: 121 MLQRARNLPSMCGIRPQQCYF 142
           MLQ+ARNLPSMCGIRPQ+C F
Sbjct: 121 MLQKARNLPSMCGIRPQRCDF 141

[Close]

BLAST of Lsi05G006260 vs. Swiss-Prot
Match: 2SS_RICCO (2S albumin OS=Ricinus communis PE=1 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 3.9e-16
Identity = 57/149 (38.26%), Postives = 95/149 (63.76%), Query Frame = 1


Query: 1   MARLTSIIAFLAVAL-LVADA-YAHRTTITTVEVDEDNQRRQ----ERCRQMRAREEIGS 60
           MA+L   IA ++V L ++A+A +A+RTTITT+E+DE    R+    ++CRQ   R+++ S
Sbjct: 1   MAKLIPTIALVSVLLFIIANASFAYRTTITTIEIDESKGEREGSSSQQCRQEVQRKDLSS 60

Query: 61  CAQYLSQQS------RYVLQMRGIDNQRRREGHAFEECCNELRNVDEECRCELLEEIAME 120
           C +YL Q S        VL+M G +NQ++ E    ++CCN+++ V +EC+CE ++ IA +
Sbjct: 61  CERYLRQSSSRRSPGEEVLRMPGDENQQQ-ESQQLQQCCNQVKQVRDECQCEAIKYIAED 120

Query: 121 --EQRRARGQEGRQMLQRARNLPSMCGIR 136
             +Q +  G+E  ++ QRA  + S CG+R
Sbjct: 121 QIQQGQLHGEESERVAQRAGEIVSSCGVR 148

[Close]

BLAST of Lsi05G006260 vs. Swiss-Prot
Match: 2SS1_BEREX (2S sulfur-rich seed storage protein 1 OS=Bertholletia excelsa GN=BE2S1 PE=1 SV=2)

HSP 1 Score: 74.7 bits (182), Expect = 9.0e-13
Identity = 54/146 (36.99%), Postives = 81/146 (55.48%), Query Frame = 1


Query: 1   MARLTSIIAFLAVALLVADAYAHRTTITTVEVDEDNQRRQERCR-QMRAREEIGSCAQYL 60
           MA+++   A L V + +  A A R T+TT  V+E+NQ   E CR QM+ ++ +  C  Y+
Sbjct: 1   MAKISVAAAALLVLMALGHATAFRATVTTTVVEEENQ---EECREQMQRQQMLSHCRMYM 60

Query: 61  SQQ---SRYVLQMRGIDNQRRREGHAFEECCNELRNVDEECRCELLEEIAM---EEQRRA 120
            QQ   S Y    R     R  E H   ECC +L  +DE CRCE L  + M   +E+ + 
Sbjct: 61  RQQMEESPYQTMPR-----RGMEPH-MSECCEQLEGMDESCRCEGLRMMMMRMQQEEMQP 120

Query: 121 RGQEGRQMLQRARNLPSMCGIRPQQC 140
           RG++ R+M++ A N+PS C + P +C
Sbjct: 121 RGEQMRRMMRLAENIPSRCNLSPMRC 137

[Close]

BLAST of Lsi05G006260 vs. Swiss-Prot
Match: 2SS2_BEREX (2S sulfur-rich seed storage protein 2 OS=Bertholletia excelsa GN=BE2S2 PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 4.9e-11
Identity = 47/146 (32.19%), Postives = 80/146 (54.79%), Query Frame = 1


Query: 1   MARLTSIIAFLAVALLVADAYAHRTTITTVEVDEDNQ----RRQERCR-QMRAREEIGSC 60
           MA+++ + A L   L++  A A RTT+TT   +E  +    R +++CR QM  ++++  C
Sbjct: 1   MAKMSVVAAALLALLVLGQATAFRTTVTTTLEEEQEENPRGRSEQQCREQMERQQQLNHC 60

Query: 61  AQYLSQQSRYVLQMRGIDNQRRREGHAFEECCNELRNVDEECRCELLEEI--AMEEQRRA 120
             YL QQ            +R  E H  +ECC +L  +DE CRCE L  +     E+   
Sbjct: 61  RMYLRQQMEESPYQNPRPLRRGEEPH-LDECCEQLERMDEMCRCEGLRMMLRRQREEMEL 120

Query: 121 RGQEGRQMLQRARNLPSMCGIRPQQC 140
           +G++ ++++++A NL S C + PQ+C
Sbjct: 121 QGEQMQRIMRKAENLLSRCNLSPQRC 145

[Close]

BLAST of Lsi05G006260 vs. Swiss-Prot
Match: 2SS5_HELAN (2S seed storage protein OS=Helianthus annuus GN=HAG5 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 7.1e-10
Identity = 45/147 (30.61%), Postives = 79/147 (53.74%), Query Frame = 1


Query: 7   IIAFLAVALLVADAYAHRTTITTVEVDEDNQRRQERCRQMRAREEIGSCAQYLSQQSRYV 66
           I+  LA A LVA A AH T ITT   DE+    Q +  Q    + +  C  +L Q     
Sbjct: 5   IVLALAFAALVAFATAHTTIITTTIEDENPISGQRQVSQRIQGQRLNQCRMFLQQGQNIP 64

Query: 67  LQMRGIDNQR--RREGHAFEECCNELRNVDEECRCELLEEIAMEEQRRARGQEGRQML-- 126
              R  DN +  R++    ++CC EL+N++ +C+CE ++++  E Q++ + Q+GRQ++  
Sbjct: 65  ---REFDNPQMGRQQEQQLQQCCQELQNIEGQCQCEAVKQVFREAQQQVQQQQGRQLVPF 124

Query: 127 ----------QRARNLPSMCGIRPQQC 140
                     Q+A+ LP++C ++ ++C
Sbjct: 125 RGSQQTQQLKQKAQILPNVCNLQSRRC 148

[Close]

BLAST of Lsi05G006260 vs. TrEMBL
Match: A0A0A0L7Q2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G080340 PE=4 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 5.1e-55
Identity = 117/141 (82.98%), Postives = 127/141 (90.07%), Query Frame = 1


Query: 1   MARLTSIIAFLAVALLVADAYAHRTTITTVEVDEDNQRRQERCRQMRAREEIGSCAQYLS 60
           MARLTSIIA LA+AL++ADA  HRTTITTVEVDE+N  R ERCRQMRAREEIGSC QYLS
Sbjct: 1   MARLTSIIALLALALVIADA--HRTTITTVEVDEENPGRMERCRQMRAREEIGSCGQYLS 60

Query: 61  QQSRYVLQMRGIDNQRRREGHAFEECCNELRNVDEECRCELLEEIAMEEQRRARGQEGRQ 120
           QQSRYVLQMRGIDNQRRR G  F+ECC+EL NV+EECRCELL+EIAMEEQRRARGQE  Q
Sbjct: 61  QQSRYVLQMRGIDNQRRRGGQLFDECCSELTNVEEECRCELLQEIAMEEQRRARGQERTQ 120

Query: 121 MLQRARNLPSMCGIRPQQCYF 142
           MLQ A+NLPSMCG+RPQQCYF
Sbjct: 121 MLQTAKNLPSMCGMRPQQCYF 139

[Close]

BLAST of Lsi05G006260 vs. TrEMBL
Match: A0A0U1Z284_TRIKI (Alpha-amylase inhibitor OS=Trichosanthes kirilowii GN=AAI PE=2 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 2.0e-51
Identity = 108/141 (76.60%), Postives = 125/141 (88.65%), Query Frame = 1


Query: 1   MARLTSIIAFLAVALLVADAYAHRTTITTVEVDEDNQRRQERCRQMRAREEIGSCAQYLS 60
           MARLT IIA LAVALLV +AYA+RTTITTVEVD+DNQ RQ+RCRQM ARE++ SC QYL 
Sbjct: 1   MARLTGIIALLAVALLVVEAYAYRTTITTVEVDDDNQGRQQRCRQMSAREKLRSCEQYLR 60

Query: 61  QQSRYVLQMRGIDNQRRREGHAFEECCNELRNVDEECRCELLEEIAMEEQRRARGQEGRQ 120
           QQSR VL MRGIDN RR+EG +F+ECC+ELRNVDE+CRC+LLEEIA EEQR+ARGQEGRQ
Sbjct: 61  QQSRDVLLMRGIDNPRRKEGGSFDECCSELRNVDEQCRCDLLEEIAREEQRQARGQEGRQ 120

Query: 121 MLQRARNLPSMCGIRPQQCYF 142
           +L +ARNLPSMCG+RP +C F
Sbjct: 121 LLDKARNLPSMCGLRPDRCAF 141

[Close]

BLAST of Lsi05G006260 vs. TrEMBL
Match: Q8L694_MOMCH (Napin OS=Momordica charantia GN=napin PE=2 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 2.1e-37
Identity = 84/141 (59.57%), Postives = 109/141 (77.30%), Query Frame = 1


Query: 1   MARLTSIIAFLAVALLVADAYAHRTTITTVEVDEDNQRRQERCRQMRAREEIGSCAQYLS 60
           MARL+S++  LAVALL+ D YA+RTTITTVEVDEDNQ R ERC  +R RE++ SC  +L 
Sbjct: 1   MARLSSMVVLLAVALLLTDVYAYRTTITTVEVDEDNQGRHERCHHIRPREQLRSCESFL- 60

Query: 61  QQSRYVLQMRGIDNQRRREGHAFEECCNELRNVDEECRCELLEEIAMEEQRRARGQEGRQ 120
           +QSR  L+M+G++  +       EECC +LRNV+E+CRC+ L+EIA E QR+ RGQEG Q
Sbjct: 61  RQSRGYLEMKGVEENQWERQQGLEECCRQLRNVEEQCRCDALQEIAREVQRQERGQEGSQ 120

Query: 121 MLQRARNLPSMCGIRPQQCYF 142
           MLQ+AR LP+MCG+RPQ+C F
Sbjct: 121 MLQKARMLPAMCGVRPQRCDF 140

[Close]

BLAST of Lsi05G006260 vs. TrEMBL
Match: A0A0M5WZ27_MOMCH (AdMc1 protein OS=Momordica charantia GN=adMc1 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 4.4e-35
Identity = 87/141 (61.70%), Postives = 107/141 (75.89%), Query Frame = 1


Query: 1   MARLTSIIAFLAVALLVADAYAHRTTITTVEVDEDNQRRQERCRQMRAREEIGSCAQYLS 60
           MARL+SIIA  AVALL+ADAYA+RTTITTVEVDEDNQ RQERCR +R RE++ SC  +L 
Sbjct: 1   MARLSSIIALFAVALLIADAYAYRTTITTVEVDEDNQGRQERCRHIRPREQLRSCQDFLR 60

Query: 61  QQSRYVLQMRGIDNQRRREGHAFEECCNELRNVDEECRCELLEEIAMEEQRRARGQEGRQ 120
           QQ     ++  ++NQ  RE    EECC +LRNV+E+CRC+ LEE+A E Q +  GQ+G Q
Sbjct: 61  QQGGGRREI--LENQWGRE-QGLEECCRQLRNVEEQCRCDALEEVAREVQSQQHGQQGSQ 120

Query: 121 MLQRARNLPSMCGIRPQQCYF 142
           +LQ AR LPSMC IRPQ+C F
Sbjct: 121 ILQHARMLPSMCQIRPQRCDF 138

[Close]

BLAST of Lsi05G006260 vs. TrEMBL
Match: D0PWG2_CORAV (2S albumin OS=Corylus avellana PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.4e-20
Identity = 64/144 (44.44%), Postives = 92/144 (63.89%), Query Frame = 1


Query: 1   MARLTSIIAFLAVALLVADAYAHRTTITTVEVDED--NQ--RRQERCRQMRAREE-IGSC 60
           MARL ++ A  A  LLVA A A RTTITTV+VDED  NQ  RR E CR+   R++ +  C
Sbjct: 1   MARLATLAALFAALLLVAHAAAFRTTITTVDVDEDIVNQQGRRGESCREQAQRQQNLNQC 60

Query: 61  AQYLSQQSRYVLQMRGIDNQRRREGHAFEECCNELRNVDEECRCELLEEIAMEEQRRARG 120
            +Y+ QQS+Y       D   +++    E+CC +LR +DE CRCE L +  M++Q   RG
Sbjct: 61  QRYMRQQSQY----GSYDGSNQQQQQELEQCCQQLRQMDERCRCEGLRQAVMQQQGEMRG 120

Query: 121 QEGRQMLQRARNLPSMCGIRPQQC 140
           +E R++++ AR+LP+ C + PQ+C
Sbjct: 121 EEMREVMETARDLPNQCRLSPQRC 140

[Close]

BLAST of Lsi05G006260 vs. TAIR10
Match: AT5G54740.1 (AT5G54740.1 seed storage albumin 5)

HSP 1 Score: 52.4 bits (124), Expect = 2.7e-07
Identity = 47/154 (30.52%), Postives = 83/154 (53.90%), Query Frame = 1


Query: 1   MARLTSIIAFLAVALLVADAYAHRTTITTVEVDEDNQRRQERC-RQMRAREEIGSCAQYL 60
           MA+L  + A LA+ +L+A+A  +RT +   E D+ +  +Q +C R+    +++  C Q++
Sbjct: 1   MAKLILVFATLALFILLANASIYRTVVEFEEDDDVSNPQQGKCQREFMKHQQLRGCKQWI 60

Query: 61  ---SQQSRY---------VLQMRGIDNQRR---REGHAFEECCNELRNVDEECRCELLEE 120
              +QQ R           L +   D++     ++  + + CCNELR VD+ C C  L++
Sbjct: 61  RKRAQQGRIGYEADDFELTLDVDLEDDENPMGPQQQSSLKMCCNELRQVDKMCVCPTLKK 120

Query: 121 IAMEEQRRARGQEGRQMLQR----ARNLPSMCGI 135
            A  +Q R +G  G+Q +Q     A+NLP++C I
Sbjct: 121 AA--QQVRFQGMHGQQQVQHVFQTAKNLPNVCKI 152

[Close]

BLAST of Lsi05G006260 vs. NCBI nr
Match: gi|778676144|ref|XP_011650534.1| (PREDICTED: 2S albumin-like [Cucumis sativus])

HSP 1 Score: 221.9 bits (564), Expect = 7.3e-55
Identity = 117/141 (82.98%), Postives = 127/141 (90.07%), Query Frame = 1


Query: 1   MARLTSIIAFLAVALLVADAYAHRTTITTVEVDEDNQRRQERCRQMRAREEIGSCAQYLS 60
           MARLTSIIA LA+AL++ADA  HRTTITTVEVDE+N  R ERCRQMRAREEIGSC QYLS
Sbjct: 1   MARLTSIIALLALALVIADA--HRTTITTVEVDEENPGRMERCRQMRAREEIGSCGQYLS 60

Query: 61  QQSRYVLQMRGIDNQRRREGHAFEECCNELRNVDEECRCELLEEIAMEEQRRARGQEGRQ 120
           QQSRYVLQMRGIDNQRRR G  F+ECC+EL NV+EECRCELL+EIAMEEQRRARGQE  Q
Sbjct: 61  QQSRYVLQMRGIDNQRRRGGQLFDECCSELTNVEEECRCELLQEIAMEEQRRARGQERTQ 120

Query: 121 MLQRARNLPSMCGIRPQQCYF 142
           MLQ A+NLPSMCG+RPQQCYF
Sbjct: 121 MLQTAKNLPSMCGMRPQQCYF 139

[Close]

BLAST of Lsi05G006260 vs. NCBI nr
Match: gi|68564970|sp|Q39649.1|2SS_CUCMA (RecName: Full=2S albumin; Contains: RecName: Full=2S albumin small chain; Contains: RecName: Full=2S albumin large chain; Flags: Precursor [Cucurbita maxima])

HSP 1 Score: 213.4 bits (542), Expect = 2.6e-52
Identity = 112/141 (79.43%), Postives = 126/141 (89.36%), Query Frame = 1


Query: 1   MARLTSIIAFLAVALLVADAYAHRTTITTVEVDEDNQRRQERCRQMRAREEIGSCAQYLS 60
           MARLTSIIA  AVALLVADAYA+RTTITTVEV+E+ Q R+ERCRQM AREE+ SC QYL 
Sbjct: 1   MARLTSIIALFAVALLVADAYAYRTTITTVEVEENRQGREERCRQMSAREELRSCEQYLR 60

Query: 61  QQSRYVLQMRGIDNQRRREGHAFEECCNELRNVDEECRCELLEEIAMEEQRRARGQEGRQ 120
           QQSR VLQMRGI+N  RREG +F+ECC EL+NVDEECRC++LEEIA EEQR+ARGQEGRQ
Sbjct: 61  QQSRDVLQMRGIENPWRREGGSFDECCRELKNVDEECRCDMLEEIAREEQRQARGQEGRQ 120

Query: 121 MLQRARNLPSMCGIRPQQCYF 142
           MLQ+ARNLPSMCGIRPQ+C F
Sbjct: 121 MLQKARNLPSMCGIRPQRCDF 141

[Close]

BLAST of Lsi05G006260 vs. NCBI nr
Match: gi|758343810|gb|AJO68010.1| (alpha-amylase inhibitor [Trichosanthes kirilowii])

HSP 1 Score: 209.9 bits (533), Expect = 2.9e-51
Identity = 108/141 (76.60%), Postives = 125/141 (88.65%), Query Frame = 1


Query: 1   MARLTSIIAFLAVALLVADAYAHRTTITTVEVDEDNQRRQERCRQMRAREEIGSCAQYLS 60
           MARLT IIA LAVALLV +AYA+RTTITTVEVD+DNQ RQ+RCRQM ARE++ SC QYL 
Sbjct: 1   MARLTGIIALLAVALLVVEAYAYRTTITTVEVDDDNQGRQQRCRQMSAREKLRSCEQYLR 60

Query: 61  QQSRYVLQMRGIDNQRRREGHAFEECCNELRNVDEECRCELLEEIAMEEQRRARGQEGRQ 120
           QQSR VL MRGIDN RR+EG +F+ECC+ELRNVDE+CRC+LLEEIA EEQR+ARGQEGRQ
Sbjct: 61  QQSRDVLLMRGIDNPRRKEGGSFDECCSELRNVDEQCRCDLLEEIAREEQRQARGQEGRQ 120

Query: 121 MLQRARNLPSMCGIRPQQCYF 142
           +L +ARNLPSMCG+RP +C F
Sbjct: 121 LLDKARNLPSMCGLRPDRCAF 141

[Close]

BLAST of Lsi05G006260 vs. NCBI nr
Match: gi|21327881|emb|CAD32938.1| (napin [Momordica charantia])

HSP 1 Score: 163.3 bits (412), Expect = 3.1e-37
Identity = 84/141 (59.57%), Postives = 109/141 (77.30%), Query Frame = 1


Query: 1   MARLTSIIAFLAVALLVADAYAHRTTITTVEVDEDNQRRQERCRQMRAREEIGSCAQYLS 60
           MARL+S++  LAVALL+ D YA+RTTITTVEVDEDNQ R ERC  +R RE++ SC  +L 
Sbjct: 1   MARLSSMVVLLAVALLLTDVYAYRTTITTVEVDEDNQGRHERCHHIRPREQLRSCESFL- 60

Query: 61  QQSRYVLQMRGIDNQRRREGHAFEECCNELRNVDEECRCELLEEIAMEEQRRARGQEGRQ 120
           +QSR  L+M+G++  +       EECC +LRNV+E+CRC+ L+EIA E QR+ RGQEG Q
Sbjct: 61  RQSRGYLEMKGVEENQWERQQGLEECCRQLRNVEEQCRCDALQEIAREVQRQERGQEGSQ 120

Query: 121 MLQRARNLPSMCGIRPQQCYF 142
           MLQ+AR LP+MCG+RPQ+C F
Sbjct: 121 MLQKARMLPAMCGVRPQRCDF 140

[Close]

BLAST of Lsi05G006260 vs. NCBI nr
Match: gi|930584849|emb|CDG50933.1| (AdMc1 protein [Momordica charantia])

HSP 1 Score: 155.6 bits (392), Expect = 6.4e-35
Identity = 87/141 (61.70%), Postives = 107/141 (75.89%), Query Frame = 1


Query: 1   MARLTSIIAFLAVALLVADAYAHRTTITTVEVDEDNQRRQERCRQMRAREEIGSCAQYLS 60
           MARL+SIIA  AVALL+ADAYA+RTTITTVEVDEDNQ RQERCR +R RE++ SC  +L 
Sbjct: 1   MARLSSIIALFAVALLIADAYAYRTTITTVEVDEDNQGRQERCRHIRPREQLRSCQDFLR 60

Query: 61  QQSRYVLQMRGIDNQRRREGHAFEECCNELRNVDEECRCELLEEIAMEEQRRARGQEGRQ 120
           QQ     ++  ++NQ  RE    EECC +LRNV+E+CRC+ LEE+A E Q +  GQ+G Q
Sbjct: 61  QQGGGRREI--LENQWGRE-QGLEECCRQLRNVEEQCRCDALEEVAREVQSQQHGQQGSQ 120

Query: 121 MLQRARNLPSMCGIRPQQCYF 142
           +LQ AR LPSMC IRPQ+C F
Sbjct: 121 ILQHARMLPSMCQIRPQRCDF 138

The following BLAST results are available for this feature:

BLAST of Lsi05G006260 vs. Swiss-Prot


Match Name	E-value	Identity	Description
2SS_CUCMA	1.6e-54	79.43	2S albumin OS=Cucurbita maxima PE=1 SV=1	[more]
2SS_RICCO	3.9e-16	38.26	2S albumin OS=Ricinus communis PE=1 SV=2	[more]
2SS1_BEREX	9.0e-13	36.99	2S sulfur-rich seed storage protein 1 OS=Bertholletia excelsa GN=BE2S1 PE=1 SV=2	[more]
2SS2_BEREX	4.9e-11	32.19	2S sulfur-rich seed storage protein 2 OS=Bertholletia excelsa GN=BE2S2 PE=3 SV=1	[more]
2SS5_HELAN	7.1e-10	30.61	2S seed storage protein OS=Helianthus annuus GN=HAG5 PE=1 SV=1	[more]

BLAST of Lsi05G006260 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L7Q2_CUCSA	5.1e-55	82.98	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G080340 PE=4 SV=1	[more]
A0A0U1Z284_TRIKI	2.0e-51	76.60	Alpha-amylase inhibitor OS=Trichosanthes kirilowii GN=AAI PE=2 SV=1	[more]
Q8L694_MOMCH	2.1e-37	59.57	Napin OS=Momordica charantia GN=napin PE=2 SV=1	[more]
A0A0M5WZ27_MOMCH	4.4e-35	61.70	AdMc1 protein OS=Momordica charantia GN=adMc1 PE=2 SV=1	[more]
D0PWG2_CORAV	1.4e-20	44.44	2S albumin OS=Corylus avellana PE=2 SV=1	[more]

BLAST of Lsi05G006260 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G54740.1	2.7e-07	30.52	seed storage albumin 5	[more]

BLAST of Lsi05G006260 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778676144\|ref\|XP_011650534.1\|	7.3e-55	82.98	PREDICTED: 2S albumin-like [Cucumis sativus]	[more]
gi\|68564970\|sp\|Q39649.1\|2SS_CUCMA	2.6e-52	79.43	RecName: Full=2S albumin; Contains: RecName: Full=2S albumin small chain; Contai...	[more]
gi\|758343810\|gb\|AJO68010.1\|	2.9e-51	76.60	alpha-amylase inhibitor [Trichosanthes kirilowii]	[more]
gi\|21327881\|emb\|CAD32938.1\|	3.1e-37	59.57	napin [Momordica charantia]	[more]
gi\|930584849\|emb\|CDG50933.1\|	6.4e-35	61.70	AdMc1 protein [Momordica charantia]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR016140	Bifunc_inhib/LTP/seed_store
IPR013771	Bifunctional trypsin/alpha-amylase inhibitor helical domain

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0008150	biological_process
cellular_component	GO:0033095	aleurone grain
cellular_component	GO:0000322	storage vacuole
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0015066	alpha-amylase inhibitor activity
molecular_function	GO:0045735	nutrient reservoir activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G006260.1	Lsi05G006260.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013771	Bifunctional trypsin/alpha-amylase inhibitor helical domain	GENE3D	G3DSA:1.10.120.10		coord: 42..140 score: 9.6
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF00234	Tryp_alpha_amyl	coord: 55..139 score: 5.1
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	SMART	SM00499	aai_6	coord: 55..139 score: 3.
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	unknown	SSF47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 10..140 score: 6.94
None	No IPR available	PANTHER	PTHR35496	FAMILY NOT NAMED	coord: 1..141 score: 1.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G006260	Csa3G080340	Cucumber (Chinese Long) v2	culsiB198
Lsi05G006260	CSPI03G05360	Wild cucumber (PI 183967)	cpilsiB197
Lsi05G006260	Cucsa.059740	Cucumber (Gy14) v1	cgylsiB048
Lsi05G006260	CmaCh04G009390	Cucurbita maxima (Rimu)	cmalsiB691
Lsi05G006260	CmoCh16G009130	Cucurbita moschata (Rifu)	cmolsiB301
Lsi05G006260	CmoCh04G010090	Cucurbita moschata (Rifu)	cmolsiB684
Lsi05G006260	Cla005277	Watermelon (97103) v1	lsiwmB348
Lsi05G006260	MELO3C025418	Melon (DHL92) v3.5.1	lsimeB370
Lsi05G006260	ClCG10G005680	Watermelon (Charleston Gray)	lsiwcgB318
Lsi05G006260	MELO3C025418.2	Melon (DHL92) v3.6.1	lsimedB388
Lsi05G006260	Cla97C10G189900	Watermelon (97103) v2	lsiwmbB322
Lsi05G006260	Bhi11G000518	Wax gourd	lsiwgoB410
Lsi05G006260	Cp4.1LG01g08070	Cucurbita pepo (Zucchini)	cpelsiB370
Lsi05G006260	Cp4.1LG00g00130	Cucurbita pepo (Zucchini)	cpelsiB014
Lsi05G006260	CsGy3G005440	Cucumber (Gy14) v2	cgyblsiB183
Lsi05G006260	Carg24822	Silver-seed gourd	carlsiB729

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G006260	Silver-seed gourd	carlsiB616
Lsi05G006260	Silver-seed gourd	carlsiB827
Lsi05G006260	Cucumber (Chinese Long) v3	cuclsiB217
Lsi05G006260	Bottle gourd (USVL1VR-Ls)	lsilsiB136
Lsi05G006260	Cucurbita maxima (Rimu)	cmalsiB030
Lsi05G006260	Cucurbita maxima (Rimu)	cmalsiB315
Lsi05G006260	Cucurbita moschata (Rifu)	cmolsiB018