Lsi05G002880 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G002880
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	E3 ubiquitin-protein ligase
Location	chr05 : 3706987 .. 3710821 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCCATTGTTTTGTTTCTATATATATCCTAAAATCTTCCCACCGAAATACTCAACTTGGAATCAGCTCCCGAAAAATCCATGGCGACGAAGATGGTTCAAAATGACCAAGACGCTCAACCAGAGCAAGCTGAAGAAGAAAATCCGGCCGTGAATACGCCGGAACCGGAGACCAACCTGAAAACGTCCGGCAAGAAAAGGAGAATCGAGTCTCAAAATGTAACATTAATCGATCCTGAAATTCTCGACTGTTATATTTGCTGCGAGCCATTGGCCATTCCGGTTTTCCAGGTCCGTTTTCTTCACCCATTCTGTCTAAATCTTCTTTCTGATTATCGTATTTCGACTACTAATTTAGGGGAAATTTGAAGCAATGTTTTTGCCCTAATTTATTTAGTACTGTATTTGTGCATGTTGATGGGAGATTCATCTGCTTTTACTGCGTTTCTAAGTACCATTGCTTTGTTTGCTCTCCTGAAGCTTAAATTGTCTGGCATGAATTATATGCATGCTGCTCTGTAAATGTTGAACCTGAAAAACTGGACTACCGTATGAATTTGAACCTAAACCCACATTTGTTTCATGGATTTGTCATTCTTAGGCTTTGTTTTGGGACATTTCTAGTAACCTCCAAGTAGCTACTCCCTAGGTTCACACTTTGAAATAATTCTTCATGTTGTAAGACTTCAGATCAATTAATGGGAGTTGTACTCATACTACTACTCTGTAGCAAATTTGGCTGGATTTGTTGGACACATTCACTGGTTTCAATGACGGTTCACAACCCAAATCCTTGAACCTTAGTTAAAACTTAAACCCATAGTGTCTCCAAACATGATCACATATGGCTGCCAAATTATGGTAAAAGTTGTTCAAATGTGGTGCAACATGACCACCACATTGTAGTTTCTCTCTAAACTTTTCAGGTTGAAGAAATAATTATGTGGGACTCATGGAATAAAATAATATATCATCCTCCAATATTCATTGCTTACCATAAAAGTAGCATTTAATAATTACGTGGGACCCATGATGTAAATTAATATATCATTTTTTTAGTACAATTGGGAGTAGAGGGATTTAAACAACAAATCTCGTGGTTTCTAGCACACTTATATGCAAATTGAGCTATGCTCGGTTTGTCAAATAATATATCATCTAACAACATGTTAAATGCTGGATTAGATGGGAGGAGTATGTACTTTTTGAAGTGATTAAGGAGTATTCGCACATGAAACATATGCAGTTCATGTTATTTTTCTTTAATGTCAATCTGTTGTTATTTAACTATGCCGCTAAGTTTAAAACTTAAGCTTATTCATTAGGATAGGTTTAGTGTGTATATATATATATATTCTTAACAATCTCTTTCATCGATTTAAATTGTTTGGTCTCTAAGTTTTTAGTATAGTGTCAATATGGTTTTTAGATTTTAAAAGCTACATTTTACCCGAAGGTTTGAGTTTATTTTTGTGTGGTCCCAAGGTTACAAAATACATTACACTTAGTTTTGAGTTTAGTTTCCATTTGGTCCCTACTTTTACCTTTGAATTTTTATTGATGCTCGCTTTTGGTCTTTGACTCTTCGTTATAAACTTAGCTAATCAATTTAAAATAATTACGATGTGAACTTTAATTAATTTTTTAAAAAAAATACTTAAGTGCATCTGGGCAGCACCTATCTTTGTACATTCTACAAAATTGTTCAATCATAATCTGTCATATGACAAATTTTTCTTATTTCTTTTTTGAAATATTTTAATTATTTTTTGGTGTGAGTGATGATAGAGGGATGTATAAAGATGAGTGCATCTCTACCCTTAATTTTAATAGTGATGGAAAAGCAATGAAAAATAATATAATTCTTTAAAATTATGGTAAAATATCTTTTTGGTCCCTAGGTTTCAAAATGTTACATATTTAGTCCTTTAAGTTTTGATTTTGGTTTCAATTTAGACCTTTAGTTTAAAAATGTTACAATGTTACCCTTGAGATTTGATTTTTATTTCAATTTGGTCCTTTTTATTTTTAACCTCAATTTTTCATTAAAGACTCACTTCCAATCATTAGTGTCAATATCTATTAATTAATTTACAATATTCTGAAGTGAAATTTTAAATTTAATTTTAAAAGTGATAAAAAATAGTGAAATTTAATTAATTATAATTCTCTTAATTCTTTTAAATTTATTAAGATACTTTAACACTAAAGATAGAAAATGAGTATTTAGTGAAAAATTGAGGTTAAAAATGTAAACTCTTTAAACTTGGACCAAATTGAAACAAAAATCAAATCTCAAGGGTAAAATTGTAACATTTTGAAACTTAGAGACTAAATTGAAACCAAAATCAAAACTTAAGGACTAAATGTGTAACATTTTGAAACCTAGGGACCAAATAGAAGCTAGACTCAAAACCTAGGGACCAAAAAGGTATTTTACTCTTAAAATTAACATAGATGACTAAAAATGAGCAGTAGCGAAACACTCAATGATAACACTGTAAAATATTGAAATATAGGACAAAATAAAAATTAAACTCATAACTCAAGGATGAAAATGTAACATTTTGAAATTAGACCAAATAGAAACTAAACTAAAAACTTAGAGCAAAAGTATAACATTTTAAAAACCTGGGAGTAAATAGAAATCATAAAAAACTTGGGATCAAAAAGTTTTTTTTTTCCTAGTTCAAACATAGGACCTTGTATGGTGATATCATATCATTGAAATATTCATGTTTCCTATAAAAAAAATTAAAAATGTGGAAAAGGAAAAGAAAGTAATAGCTAAGAATATCTTTATATGGTTCATAATATCTCAATTCTTCTATACTATATATGGTCTTAACAAGACAATGATATTTCTGTGGTTTTAGTGTGAAAATGGACATGTAGCATGCTCTGATTGTTGTAGTAAAAATGTGGAAGAATGCCCCTTTTGTCATAAGAAAATAGGGGACATCCGTTGTCGAGCCATTGAGACTGTTTTGGAATCACTCAAAGGAGCTTGTCAAAACTCAAAGTATGGCTGCAAAGCAAAGCCCACATTTGATCATAGAAAAGAGCATTGGAACAAATGTTCATTTAGTCCATGTCGTTGCCCTATATCCAACTGTGATTACGTAGGGCCATCGAAGAAATTATACACACATTTCAACCAGAAACACCCAAGTTCAGCCATTAACTTCCATTACTATTCTAGCTTCACCATCAATCTGAAACAGGACGACAAGTTTCTCGTCCTGCGAGAAAAGAGAGCTGGAGTTCTCTTTGTGCTTAGCAGTAGAACAACTCAGCCTCTTGGAAAGGCGATCACCGTGCACTCTATCGGTTTTGCCTCTAGCAAGGAGGAGGAAGAGGAAGGGCTTTCACTAGAACTGGTTCTGAAAACTAAAGAAACCACACTTAGGTTGCAATCAGTAATGAAGGGAATTAAGGAGTTGGTAGATGAGATGCCTCCAGAGGAGTTTCTTGTGATTCCCAACAAGTTTTTTTGGTTCTGATAAACAAATCAAGCTGGAGCTTCGCATTTGGTAAAAGAGGGGAAAAAAGAAACAGCATCTCCTCAAGGGTTTTTTTTGGGCTGTATGTATCATATTTTGGTTTAGTTTCATATGGTGAATTAGAATTGCTTTTGATAGCTATAGTAGGATATGATATTTTGGATGATCTTCATTTTGGGGTTAGAATTTAGATGCTATTGCTGTAGGCCAGGAAAGGAATGCAAATGATCTATATCTATCTATGTGGATTATGGTATGTTTGTGAAATATTCTCTAATATGTAAAGCATGTTAGTTCAAACGGCAATTTTAATAGTGTTATTATTTTTTTTTTC

mRNA sequence

CTCCATTGTTTTGTTTCTATATATATCCTAAAATCTTCCCACCGAAATACTCAACTTGGAATCAGCTCCCGAAAAATCCATGGCGACGAAGATGGTTCAAAATGACCAAGACGCTCAACCAGAGCAAGCTGAAGAAGAAAATCCGGCCGTGAATACGCCGGAACCGGAGACCAACCTGAAAACGTCCGGCAAGAAAAGGAGAATCGAGTCTCAAAATGTAACATTAATCGATCCTGAAATTCTCGACTGTTATATTTGCTGCGAGCCATTGGCCATTCCGGTTTTCCAGTGTGAAAATGGACATGTAGCATGCTCTGATTGTTGTAGTAAAAATGTGGAAGAATGCCCCTTTTGTCATAAGAAAATAGGGGACATCCGTTGTCGAGCCATTGAGACTGTTTTGGAATCACTCAAAGGAGCTTGTCAAAACTCAAAGTATGGCTGCAAAGCAAAGCCCACATTTGATCATAGAAAAGAGCATTGGAACAAATGTTCATTTAGTCCATGTCGTTGCCCTATATCCAACTGTGATTACGTAGGGCCATCGAAGAAATTATACACACATTTCAACCAGAAACACCCAAGTTCAGCCATTAACTTCCATTACTATTCTAGCTTCACCATCAATCTGAAACAGGACGACAAGTTTCTCGTCCTGCGAGAAAAGAGAGCTGGAGTTCTCTTTGTGCTTAGCAGTAGAACAACTCAGCCTCTTGGAAAGGCGATCACCGTGCACTCTATCGGTTTTGCCTCTAGCAAGGAGGAGGAAGAGGAAGGGCTTTCACTAGAACTGGTTCTGAAAACTAAAGAAACCACACTTAGGTTGCAATCAGTAATGAAGGGAATTAAGGAGTTGGTAGATGAGATGCCTCCAGAGGAGTTTCTTGTGATTCCCAACAAGTTTTTTTGGTTCTGATAAACAAATCAAGCTGGAGCTTCGCATTTGGTAAAAGAGGGGAAAAAAGAAACAGCATCTCCTCAAGGGTTTTTTTTGGGCTGTATGTATCATATTTTGGTTTAGTTTCATATGGTGAATTAGAATTGCTTTTGATAGCTATAGTAGGATATGATATTTTGGATGATCTTCATTTTGGGGTTAGAATTTAGATGCTATTGCTGTAGGCCAGGAAAGGAATGCAAATGATCTATATCTATCTATGTGGATTATGGTATGTTTGTGAAATATTCTCTAATATGTAAAGCATGTTAGTTCAAACGGCAATTTTAATAGTGTTATTATTTTTTTTTTC

Coding sequence (CDS)

ATGGCGACGAAGATGGTTCAAAATGACCAAGACGCTCAACCAGAGCAAGCTGAAGAAGAAAATCCGGCCGTGAATACGCCGGAACCGGAGACCAACCTGAAAACGTCCGGCAAGAAAAGGAGAATCGAGTCTCAAAATGTAACATTAATCGATCCTGAAATTCTCGACTGTTATATTTGCTGCGAGCCATTGGCCATTCCGGTTTTCCAGTGTGAAAATGGACATGTAGCATGCTCTGATTGTTGTAGTAAAAATGTGGAAGAATGCCCCTTTTGTCATAAGAAAATAGGGGACATCCGTTGTCGAGCCATTGAGACTGTTTTGGAATCACTCAAAGGAGCTTGTCAAAACTCAAAGTATGGCTGCAAAGCAAAGCCCACATTTGATCATAGAAAAGAGCATTGGAACAAATGTTCATTTAGTCCATGTCGTTGCCCTATATCCAACTGTGATTACGTAGGGCCATCGAAGAAATTATACACACATTTCAACCAGAAACACCCAAGTTCAGCCATTAACTTCCATTACTATTCTAGCTTCACCATCAATCTGAAACAGGACGACAAGTTTCTCGTCCTGCGAGAAAAGAGAGCTGGAGTTCTCTTTGTGCTTAGCAGTAGAACAACTCAGCCTCTTGGAAAGGCGATCACCGTGCACTCTATCGGTTTTGCCTCTAGCAAGGAGGAGGAAGAGGAAGGGCTTTCACTAGAACTGGTTCTGAAAACTAAAGAAACCACACTTAGGTTGCAATCAGTAATGAAGGGAATTAAGGAGTTGGTAGATGAGATGCCTCCAGAGGAGTTTCTTGTGATTCCCAACAAGTTTTTTTGGTTCTGA

Protein sequence

MATKMVQNDQDAQPEQAEEENPAVNTPEPETNLKTSGKKRRIESQNVTLIDPEILDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKKIGDIRCRAIETVLESLKGACQNSKYGCKAKPTFDHRKEHWNKCSFSPCRCPISNCDYVGPSKKLYTHFNQKHPSSAINFHYYSSFTINLKQDDKFLVLREKRAGVLFVLSSRTTQPLGKAITVHSIGFASSKEEEEEGLSLELVLKTKETTLRLQSVMKGIKELVDEMPPEEFLVIPNKFFWF

Homology

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BLAST of Lsi05G002880 vs. Swiss-Prot
Match: SIL10_ARATH (E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana GN=At5g37930 PE=2 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 4.5e-40
Identity = 103/301 (34.22%), Postives = 158/301 (52.49%), Query Frame = 1


Query: 7   QNDQDAQPEQA-----EEENPAVNT---PEPETNLKTSGKKRR------------IESQN 66
           +N++DA+   A     E+E+   N    PE E    TS    R            + SQ+
Sbjct: 33  ENEEDAETSDAGSSGEEDEDETQNQGMRPESEDRGSTSDDSDREVVIEERRFGKFVNSQS 92

Query: 67  ----------VTLIDPEILDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKKI 126
                     VTL+DP++LDC ICCEPL IP+FQC+NGH+AC+ CC+K    CP C   I
Sbjct: 93  SSSSKDSPLSVTLLDPDVLDCPICCEPLKIPIFQCDNGHLACTLCCTKVRNRCPSCTLPI 152

Query: 127 GDIRCRAIETVLESLKGACQNSKYGCKAKPTFDHRKEHWNKCSFSPCRCPISNCDYVGPS 186
           G +RCRA+E V+E+ + +C N+KYGCK   ++ +R  H   C F+PC CPI +C Y G  
Sbjct: 153 GYVRCRAMEKVIEASRVSCLNAKYGCKESTSYGNRFSHEQVCVFTPCSCPILDCHYTGYY 212

Query: 187 KKLYTHFNQKHPSSAINFHYYSSFTINLKQDDKFLVLREKRAGVLFVLSSRTTQPLGKAI 246
           K L  H   +H    I+F + +  TI+L  + K  +L+E+  G + V+     Q      
Sbjct: 213 KDLNNHVRAEHKDDLISFVWNTRLTISLDLNKKTTILQEENDGHVIVV-----QVFRALH 272

Query: 247 TVH-SIGFASSKEEEEEGLSLELVLKTKETTLRLQSVMKGIKELVDEMPPEEFLVIPNKF 277
            V+ S+   +        LS  L   T ++ L+   ++K I+++ +E P + F++IP+  
Sbjct: 273 AVYVSVSCIAPLTPGVGRLSCRLAKITVDSLLKQGFMVKNIQKVTNEHPEDGFMLIPSYL 328

[Close]

BLAST of Lsi05G002880 vs. Swiss-Prot
Match: SINL4_ARATH (E3 ubiquitin-protein ligase SINA-like 4 OS=Arabidopsis thaliana GN=At1g66650 PE=2 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 6.7e-36
Identity = 95/280 (33.93%), Postives = 146/280 (52.14%), Query Frame = 1


Query: 11  DAQPEQAEEENPAVNTPEPETN---------LKTSGKKRRIESQNVTLIDPEILDCYICC 70
           D  P + EE+   V T + ++          L+T  K+R    ++VTL +  +L+C  C 
Sbjct: 31  DPSPGEEEEKTLVVLTDDSDSEEDDKPLGDVLRTCRKRRVSSPKSVTLPNSNVLECPNCF 90

Query: 71  EPLAIPVFQCENGHVACSDCCSKNVEECPFCHKKIGDIRCRAIETVLESLKGACQNSKYG 130
           +PL  P+FQC NGH+AC  CC K  + C FC   IGD+RCRA+E V+++   +C N+ YG
Sbjct: 91  DPLKKPIFQCNNGHLACFLCCIKLKKRCSFCKLPIGDVRCRAMEKVIKAGLVSCSNAIYG 150

Query: 131 CKAKPTFDHR-KEHWNKCSFSPCRCPISNCDYVGPSKKLYTHFNQKHPSSA--INFHYYS 190
           CK   T+ ++ + H   C F+PC CPI +C+Y+G  K L  HF   H  S   IN   + 
Sbjct: 151 CKQSTTYGNQLQSHEKVCVFAPCSCPIKDCNYIGFYKDLINHFRATHKVSPGDINSFVFD 210

Query: 191 S---FTINLKQDDKFLVLREKRAGVLFVLSSRTTQPLGKAITVHSIGFASSKEEEEEGLS 250
               F ++L   DK ++  E++ G LFV+       +G      ++   +    E    S
Sbjct: 211 RPVIFGLDLDSSDKMVIFVEEKQGNLFVVQGF----IGSHGVYATVSHIAPMVPEVRKFS 270

Query: 251 LELVLKTKETTLRLQSVMKGIKEL--VDEMPPEEFLVIPN 274
             L      +TLRL   +K I++L   +E P E+FL+IP+
Sbjct: 271 CSLARLRPYSTLRLGLEVKNIQKLRSQEEQPQEDFLLIPS 306

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BLAST of Lsi05G002880 vs. Swiss-Prot
Match: SINL6_ARATH (Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana GN=At5g37870 PE=3 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 4.3e-35
Identity = 91/241 (37.76%), Postives = 132/241 (54.77%), Query Frame = 1


Query: 37  GKKRRIESQNVTLIDPEILDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKKI 96
           G+KR  ++++  L D +ILDC IC + L IPVFQC NGH+ACS CC K   +CP C   +
Sbjct: 25  GRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLACSSCCPKLRNKCPACALPV 84

Query: 97  GDIRCRAIETVLESLKGACQNSKYGCKAKPTFDHRKEHWNKCSFSPCRCPISNCDYVGPS 156
           G IRCRA+E VLES+   C+ +  GC     +     H   C+FSPC CP+  C+Y G  
Sbjct: 85  GHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKICNFSPCSCPVQGCNYTGSY 144

Query: 157 KKLYTHFNQKHP--SSAINFHYYSSFTINLKQDDKFLVLREKRAGVLFVLSSRTTQPLGK 216
           K LY H++  H   S+A +F+  S     +   DK L+ R     +LFV+     +P G 
Sbjct: 145 KDLYEHYDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERVYEKKLLFVVQC-FEEPCGV 204

Query: 217 AITVHSIGFASSKEEEEEGLSLELVLKTKE-TTLRLQS-VMKGIKELVDEMPPEEFLVIP 274
            ++V  I  A S  E  E  S  L+  T E  T+  QS  +K + ++  + P + F++IP
Sbjct: 205 YVSVSCI--APSAPEVGE-FSYGLLYTTWEGVTMTYQSPKVKKVLKVSSQRPKDSFMLIP 261

[Close]

BLAST of Lsi05G002880 vs. Swiss-Prot
Match: SINL7_ARATH (E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana GN=At5g37890 PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.3e-34
Identity = 86/237 (36.29%), Postives = 123/237 (51.90%), Query Frame = 1


Query: 39  KRRIESQNVTLIDPEILDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKKIGD 98
           KR  + ++  L+D EILDC IC E   IP+FQC+NGH+ACS CC K   +CP C   +G 
Sbjct: 33  KRDAKKRSTMLMDLEILDCPICYEAFTIPIFQCDNGHLACSSCCPKLNNKCPACTSPVGH 92

Query: 99  IRCRAIETVLESLKGACQNSKYGCKAKPTFDHRKEHWNKCSFSPCRCPISNCDYVGPSKK 158
            RCRA+E+VLES+   C N+K GCK   ++     H  +C FS C CP  +C+Y    K 
Sbjct: 93  NRCRAMESVLESILIPCPNAKLGCKKNVSYGKELTHEKECMFSHCACPALDCNYTSSYKD 152

Query: 159 LYTHFNQKHPS-SAIN-FHYYSSFTINLKQDDKFLVLREKRAGVLFVLSSRTTQPLGKAI 218
           LYTH+   H   + IN F      ++ +    K L+  E     LF +     +P G  +
Sbjct: 153 LYTHYRITHMEINQINTFICDIPLSVRMNISKKILIRTEHLTNHLFAVQC-FREPYGVYV 212

Query: 219 TVHSIGFASSKEEEEEGLSLELVLKTKETTLRLQSVMKGIKELVDEMPPEEFLVIPN 274
           TV  I   SS E  +   +L   +    T +     +K + +L  + P E F++IPN
Sbjct: 213 TVSCIA-PSSPELSQYSYALSYTV-DGHTVIYQSPEVKRVLKLSFQTPQENFMLIPN 266

[Close]

BLAST of Lsi05G002880 vs. Swiss-Prot
Match: SINL5_ARATH (E3 ubiquitin-protein ligase SINA-like 5 OS=Arabidopsis thaliana GN=At1g66660 PE=2 SV=2)

HSP 1 Score: 145.6 bits (366), Expect = 8.2e-34
Identity = 97/279 (34.77%), Postives = 150/279 (53.76%), Query Frame = 1


Query: 7   QNDQDAQPEQAEEENPAVNTPEPETNLKT---SGKKRRIESQNVTLIDPEILDCYICCEP 66
           +++++ + E+ E E       E E N+ T   SG  +   SQ V L   ++LDC  CCEP
Sbjct: 35  EDEEEFEEEEEELEEEEDEEEEEEENVTTDEQSGSPK--SSQPVKLQSSDVLDCPTCCEP 94

Query: 67  LAIPVFQCENGHVACSDCCSKNVEECPFCHKKIGDIRCRAIETVLESLKGACQNSKYGCK 126
           L  P++QC NGH+ACS CC K  ++C FC   IGDIRCRA+E V+E+    C N+K+GCK
Sbjct: 95  LKRPIYQCSNGHLACSSCCQKLNKKCSFCRCNIGDIRCRAMEKVIEASIVPCPNAKHGCK 154

Query: 127 AKPTFDHRKEHWNKCSFSPCRCPISNCDYVGPSKKLYTH-FNQKHPSSAINFHYY----- 186
              T+ ++  H   C F  C CP+SNC+YV     L +H  +  H     + H+      
Sbjct: 155 ETTTYCNQSSHEKVCKFVRCSCPVSNCNYVSSYSNLKSHACSTAHVWGEDDIHFQLVIDR 214

Query: 187 -SSFTINLKQDDKFLVLREKRAGVLFVLSSRTTQPLGKAITVHSIGFASSKEEEEEGLSL 246
              F +NL +  K +V +E++ G L V+ +      G  +TV+ I   +    +   LS 
Sbjct: 215 PRIFNMNLGR-KKTVVFKEEKEGDLIVVQAFKGLE-GVYVTVNRIAHMAPGIRD---LSC 274

Query: 247 ELVLKTKETTLRLQSVMKGIKELVDEMPPEEFLV-IPNK 275
            L    + +TLR  S++K I+++ ++M  E+ L+ IP K
Sbjct: 275 SLAKLNEYSTLRSGSLVKKIQKVREKMHLEDDLMWIPPK 306

[Close]

BLAST of Lsi05G002880 vs. TrEMBL
Match: A0A067JT13_JATCU (E3 ubiquitin-protein ligase OS=Jatropha curcas GN=JCGZ_02506 PE=3 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 3.2e-53
Identity = 117/270 (43.33%), Postives = 163/270 (60.37%), Query Frame = 1


Query: 8   NDQDAQPEQAEEENPAVNTPEPETNLKTSGKKRRIESQNVTLIDPEILDCYICCEPLAIP 67
           +D D   EQ EE    V      +N++      R     VTL DPE+LDC +C E L IP
Sbjct: 68  DDDDDDEEQEEETTEVVTNQIAMSNVRAPIGPTRNGGICVTLTDPEVLDCPVCYESLTIP 127

Query: 68  VFQCENGHVACSDCCSKNVEECPFCHKKIGDIRCRAIETVLESLKGACQNSKYGCKAKPT 127
           VFQCENGH ACS CC K +++CP C   IG  RCRAIE VLES++ +CQN KYGCK    
Sbjct: 128 VFQCENGHTACSSCCKKLLQKCPTCSLPIGYNRCRAIEKVLESVRLSCQNLKYGCKEIVN 187

Query: 128 FDHRKEHWNKCSFSPCRCPISNCDYVGPSKKLYTHFNQKHPSSAINFHYYSSFTINLKQD 187
           +  + +H   CS +PC CP S C +VG S+++Y HF+ KH  +A+ F Y ++F +    +
Sbjct: 188 YSKKVDHEKICSHAPCFCPSSGCSFVGSSRQIYQHFSNKHKGAAVLFRYGNTFPVFFTLN 247

Query: 188 DKFLVLREKRAGVLFVLSSRTTQPLGKAITVHSIGFASSKEEEEEGLSLELVLKTKETTL 247
           DK  +LRE++ GVLF+L++ T + +G  ITV  IG +S K     G   EL  K + + L
Sbjct: 248 DKSRILREEKEGVLFILNN-TAEVVGNIITVSCIGPSSPK----GGYFYELAAKMEGSNL 307

Query: 248 RLQSVMKGIKELVDEMP-PEEFLVIPNKFF 277
           + QS  K I+++ D+ P  ++FLV+P  FF
Sbjct: 308 KFQSFTKNIRKVNDDDPHSQDFLVVPASFF 332

[Close]

BLAST of Lsi05G002880 vs. TrEMBL
Match: A0A0A0L9Z6_CUCSA (E3 ubiquitin-protein ligase OS=Cucumis sativus GN=Csa_3G185660 PE=3 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 7.1e-53
Identity = 111/231 (48.05%), Postives = 146/231 (63.20%), Query Frame = 1


Query: 46  NVTLIDPEILDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKKIGDIRCRAIE 105
           NV   DP+ILDCYICCEPL+IPVFQCENGH+ACS CC+K   +CP C   IG IRCRAIE
Sbjct: 28  NVIFTDPQILDCYICCEPLSIPVFQCENGHIACSSCCTKAQNKCPSCTLAIGYIRCRAIE 87

Query: 106 TVLESLKGACQNSKYGCKAKPTFDHRKEHWNKCSFSPCRCPISNCDYVGPSKKLYTHFNQ 165
            VLES+K  CQN+ YGCK     +   +H + C + PC CP+ NC +VG +++L  HF +
Sbjct: 88  KVLESIKLPCQNAIYGCKTVMGLNLINDHESLCRYEPCSCPLDNCTFVGSTEQLGLHFTK 147

Query: 166 KHPSSAINFHYYSSFTINLKQDDKFLVLREKRAGVLFVLSSRTTQPLGKAITVHSIGFAS 225
           KH  SA  F Y + FTI L   D   +L+ +  GVLF L S T +  G A+T++ IG  S
Sbjct: 148 KHKDSAKIFSYNTRFTICLNNGDTHRILKAENDGVLFFL-SYTFEIFGNAVTMNRIGPLS 207

Query: 226 SKEEEEEGLSLELVLKTKETTLRLQSVMKGIKELVDEMPPEEFLVIPNKFF 277
           S    E+    E+  KT  + L LQS+ K I+ L+   P +  L+IPN++F
Sbjct: 208 S----EKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVPPSKGSLLIPNEYF 253

[Close]

BLAST of Lsi05G002880 vs. TrEMBL
Match: U5FIU4_POPTR (E3 ubiquitin-protein ligase OS=Populus trichocarpa GN=POPTR_0017s01040g PE=3 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.6e-52
Identity = 109/228 (47.81%), Postives = 142/228 (62.28%), Query Frame = 1


Query: 48  TLIDPEILDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKKIGDIRCRAIETV 107
           TL DPE+LDC ICCEPL IPVFQC+NGH ACS CC K   +CP C   IG  RCRAIE V
Sbjct: 148 TLSDPEVLDCPICCEPLTIPVFQCDNGHTACSSCCIKLQHKCPSCTMPIGYNRCRAIEKV 207

Query: 108 LESLKGACQNSKYGCKAKPTFDHRKEHWNKCSFSPCRCPISNCDYVGPSKKLYTHFNQKH 167
           LESLK +C NS YGCK    +  + EH   C+ +PC CP+  C+Y G SK+LY H   KH
Sbjct: 208 LESLKVSCSNSSYGCKESICYSKKYEHDKSCTHAPCTCPLPACNYQGSSKRLYQHCRIKH 267

Query: 168 PSSAINFHYYSSFTINLKQDDKFLVLREKRAGVLFVLSSRTTQPLGKAITVHSIGFASSK 227
                +F + +SF +    D KF VL+E++  VLF+L++R ++ LG  ITV  +G +SSK
Sbjct: 268 LCDLTSFQFNTSFPLFFMVDHKFRVLQEEKEDVLFILTNR-SECLGNVITVSCMGPSSSK 327

Query: 228 EEEEEGLSLELVLKTKETTLRLQSVMKGIKELVDEMPPEEFLVIPNKF 276
               +G   EL  K + + +R QS  + I+  VD  P   FL++PN F
Sbjct: 328 ----QGYFYELTAKAEGSNVRFQSSTRNIQTRVDHPPSLGFLLVPNDF 370

[Close]

BLAST of Lsi05G002880 vs. TrEMBL
Match: A0A061EGM7_THECC (E3 ubiquitin-protein ligase OS=Theobroma cacao GN=TCM_019286 PE=3 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.0e-51
Identity = 112/274 (40.88%), Postives = 169/274 (61.68%), Query Frame = 1


Query: 7   QNDQDAQPEQAEEENPAVNTPEPETNLKTSG---KKRRIESQNVTLIDPEILDCYICCEP 66
           + + + + E+ EEE+   +  E E+  +      +  R  S ++TL DPE+LDC IC E 
Sbjct: 52  EEEGEEEEEEEEEEDEYYDEEEEESGAEQGAMSLETNRDGSISITLTDPEVLDCSICYEA 111

Query: 67  LAIPVFQCENGHVACSDCCSKNVEECPFCHKKIGDIRCRAIETVLESLKGACQNSKYGCK 126
           L IPVFQCENGH+ACS CC K   +CP C   IG  RCRAIE VLES+K +CQN+KYGC+
Sbjct: 112 LTIPVFQCENGHIACSICCIKIRNKCPSCATPIGYSRCRAIEKVLESVKVSCQNTKYGCR 171

Query: 127 AKPTFDHRKEHWNKCSFSPCRCPISNCDYVGPSKKLYTHFNQKHPSSAINFHYYSSFTIN 186
               +  +++H   C ++PC CP+++C++ G SK+LY HF  KH  SA  F Y  + +I 
Sbjct: 172 EAFRYSIKQKHEKICPYAPCSCPLADCNFEGSSKELYEHFRDKHKYSATRFQYDRTISIT 231

Query: 187 LKQDDKFLVLREKRAGVLFVLSSRTTQPLGKAITVHSIGFASSKEEEEEGLSLELVLK-- 246
           +  D++F +L+E++ G LF+L+++  + LG  +T+  IG       EE G   +L+ K  
Sbjct: 232 VGIDERFCILQEEQDGSLFILNNK-VETLGNVVTLSRIG-----PSEERGFFYDLMAKAL 291

Query: 247 TKETTLRLQSVMKGIKELVDEMPPEEFLVIPNKF 276
           T+ +T+RLQS  K   + VD  P   FL++P++F
Sbjct: 292 TEASTVRLQSFTKSTPKQVDNPPSLGFLLVPSQF 319

[Close]

BLAST of Lsi05G002880 vs. TrEMBL
Match: A0A061EG86_THECC (E3 ubiquitin-protein ligase OS=Theobroma cacao GN=TCM_019286 PE=3 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.0e-51
Identity = 112/274 (40.88%), Postives = 169/274 (61.68%), Query Frame = 1


Query: 7   QNDQDAQPEQAEEENPAVNTPEPETNLKTSG---KKRRIESQNVTLIDPEILDCYICCEP 66
           + + + + E+ EEE+   +  E E+  +      +  R  S ++TL DPE+LDC IC E 
Sbjct: 52  EEEGEEEEEEEEEEDEYYDEEEEESGAEQGAMSLETNRDGSISITLTDPEVLDCSICYEA 111

Query: 67  LAIPVFQCENGHVACSDCCSKNVEECPFCHKKIGDIRCRAIETVLESLKGACQNSKYGCK 126
           L IPVFQCENGH+ACS CC K   +CP C   IG  RCRAIE VLES+K +CQN+KYGC+
Sbjct: 112 LTIPVFQCENGHIACSICCIKIRNKCPSCATPIGYSRCRAIEKVLESVKVSCQNTKYGCR 171

Query: 127 AKPTFDHRKEHWNKCSFSPCRCPISNCDYVGPSKKLYTHFNQKHPSSAINFHYYSSFTIN 186
               +  +++H   C ++PC CP+++C++ G SK+LY HF  KH  SA  F Y  + +I 
Sbjct: 172 EAFRYSIKQKHEKICPYAPCSCPLADCNFEGSSKELYEHFRDKHKYSATRFQYDRTISIT 231

Query: 187 LKQDDKFLVLREKRAGVLFVLSSRTTQPLGKAITVHSIGFASSKEEEEEGLSLELVLK-- 246
           +  D++F +L+E++ G LF+L+++  + LG  +T+  IG       EE G   +L+ K  
Sbjct: 232 VGIDERFCILQEEQDGSLFILNNK-VETLGNVVTLSRIG-----PSEERGFFYDLMAKAL 291

Query: 247 TKETTLRLQSVMKGIKELVDEMPPEEFLVIPNKF 276
           T+ +T+RLQS  K   + VD  P   FL++P++F
Sbjct: 292 TEASTVRLQSFTKSTPKQVDNPPSLGFLLVPSQF 319

[Close]

BLAST of Lsi05G002880 vs. TAIR10
Match: AT5G37930.1 (AT5G37930.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 166.4 bits (420), Expect = 2.5e-41
Identity = 103/301 (34.22%), Postives = 158/301 (52.49%), Query Frame = 1


Query: 7   QNDQDAQPEQA-----EEENPAVNT---PEPETNLKTSGKKRR------------IESQN 66
           +N++DA+   A     E+E+   N    PE E    TS    R            + SQ+
Sbjct: 33  ENEEDAETSDAGSSGEEDEDETQNQGMRPESEDRGSTSDDSDREVVIEERRFGKFVNSQS 92

Query: 67  ----------VTLIDPEILDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKKI 126
                     VTL+DP++LDC ICCEPL IP+FQC+NGH+AC+ CC+K    CP C   I
Sbjct: 93  SSSSKDSPLSVTLLDPDVLDCPICCEPLKIPIFQCDNGHLACTLCCTKVRNRCPSCTLPI 152

Query: 127 GDIRCRAIETVLESLKGACQNSKYGCKAKPTFDHRKEHWNKCSFSPCRCPISNCDYVGPS 186
           G +RCRA+E V+E+ + +C N+KYGCK   ++ +R  H   C F+PC CPI +C Y G  
Sbjct: 153 GYVRCRAMEKVIEASRVSCLNAKYGCKESTSYGNRFSHEQVCVFTPCSCPILDCHYTGYY 212

Query: 187 KKLYTHFNQKHPSSAINFHYYSSFTINLKQDDKFLVLREKRAGVLFVLSSRTTQPLGKAI 246
           K L  H   +H    I+F + +  TI+L  + K  +L+E+  G + V+     Q      
Sbjct: 213 KDLNNHVRAEHKDDLISFVWNTRLTISLDLNKKTTILQEENDGHVIVV-----QVFRALH 272

Query: 247 TVH-SIGFASSKEEEEEGLSLELVLKTKETTLRLQSVMKGIKELVDEMPPEEFLVIPNKF 277
            V+ S+   +        LS  L   T ++ L+   ++K I+++ +E P + F++IP+  
Sbjct: 273 AVYVSVSCIAPLTPGVGRLSCRLAKITVDSLLKQGFMVKNIQKVTNEHPEDGFMLIPSYL 328

[Close]

BLAST of Lsi05G002880 vs. TAIR10
Match: AT1G66650.1 (AT1G66650.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 152.5 bits (384), Expect = 3.8e-37
Identity = 95/280 (33.93%), Postives = 146/280 (52.14%), Query Frame = 1


Query: 11  DAQPEQAEEENPAVNTPEPETN---------LKTSGKKRRIESQNVTLIDPEILDCYICC 70
           D  P + EE+   V T + ++          L+T  K+R    ++VTL +  +L+C  C 
Sbjct: 31  DPSPGEEEEKTLVVLTDDSDSEEDDKPLGDVLRTCRKRRVSSPKSVTLPNSNVLECPNCF 90

Query: 71  EPLAIPVFQCENGHVACSDCCSKNVEECPFCHKKIGDIRCRAIETVLESLKGACQNSKYG 130
           +PL  P+FQC NGH+AC  CC K  + C FC   IGD+RCRA+E V+++   +C N+ YG
Sbjct: 91  DPLKKPIFQCNNGHLACFLCCIKLKKRCSFCKLPIGDVRCRAMEKVIKAGLVSCSNAIYG 150

Query: 131 CKAKPTFDHR-KEHWNKCSFSPCRCPISNCDYVGPSKKLYTHFNQKHPSSA--INFHYYS 190
           CK   T+ ++ + H   C F+PC CPI +C+Y+G  K L  HF   H  S   IN   + 
Sbjct: 151 CKQSTTYGNQLQSHEKVCVFAPCSCPIKDCNYIGFYKDLINHFRATHKVSPGDINSFVFD 210

Query: 191 S---FTINLKQDDKFLVLREKRAGVLFVLSSRTTQPLGKAITVHSIGFASSKEEEEEGLS 250
               F ++L   DK ++  E++ G LFV+       +G      ++   +    E    S
Sbjct: 211 RPVIFGLDLDSSDKMVIFVEEKQGNLFVVQGF----IGSHGVYATVSHIAPMVPEVRKFS 270

Query: 251 LELVLKTKETTLRLQSVMKGIKEL--VDEMPPEEFLVIPN 274
             L      +TLRL   +K I++L   +E P E+FL+IP+
Sbjct: 271 CSLARLRPYSTLRLGLEVKNIQKLRSQEEQPQEDFLLIPS 306

[Close]

BLAST of Lsi05G002880 vs. TAIR10
Match: AT5G37870.1 (AT5G37870.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 149.8 bits (377), Expect = 2.4e-36
Identity = 91/241 (37.76%), Postives = 132/241 (54.77%), Query Frame = 1


Query: 37  GKKRRIESQNVTLIDPEILDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKKI 96
           G+KR  ++++  L D +ILDC IC + L IPVFQC NGH+ACS CC K   +CP C   +
Sbjct: 25  GRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLACSSCCPKLRNKCPACALPV 84

Query: 97  GDIRCRAIETVLESLKGACQNSKYGCKAKPTFDHRKEHWNKCSFSPCRCPISNCDYVGPS 156
           G IRCRA+E VLES+   C+ +  GC     +     H   C+FSPC CP+  C+Y G  
Sbjct: 85  GHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKICNFSPCSCPVQGCNYTGSY 144

Query: 157 KKLYTHFNQKHP--SSAINFHYYSSFTINLKQDDKFLVLREKRAGVLFVLSSRTTQPLGK 216
           K LY H++  H   S+A +F+  S     +   DK L+ R     +LFV+     +P G 
Sbjct: 145 KDLYEHYDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERVYEKKLLFVVQC-FEEPCGV 204

Query: 217 AITVHSIGFASSKEEEEEGLSLELVLKTKE-TTLRLQS-VMKGIKELVDEMPPEEFLVIP 274
            ++V  I  A S  E  E  S  L+  T E  T+  QS  +K + ++  + P + F++IP
Sbjct: 205 YVSVSCI--APSAPEVGE-FSYGLLYTTWEGVTMTYQSPKVKKVLKVSSQRPKDSFMLIP 261

[Close]

BLAST of Lsi05G002880 vs. TAIR10
Match: AT5G37890.1 (AT5G37890.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 148.3 bits (373), Expect = 7.1e-36
Identity = 86/237 (36.29%), Postives = 123/237 (51.90%), Query Frame = 1


Query: 39  KRRIESQNVTLIDPEILDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKKIGD 98
           KR  + ++  L+D EILDC IC E   IP+FQC+NGH+ACS CC K   +CP C   +G 
Sbjct: 33  KRDAKKRSTMLMDLEILDCPICYEAFTIPIFQCDNGHLACSSCCPKLNNKCPACTSPVGH 92

Query: 99  IRCRAIETVLESLKGACQNSKYGCKAKPTFDHRKEHWNKCSFSPCRCPISNCDYVGPSKK 158
            RCRA+E+VLES+   C N+K GCK   ++     H  +C FS C CP  +C+Y    K 
Sbjct: 93  NRCRAMESVLESILIPCPNAKLGCKKNVSYGKELTHEKECMFSHCACPALDCNYTSSYKD 152

Query: 159 LYTHFNQKHPS-SAIN-FHYYSSFTINLKQDDKFLVLREKRAGVLFVLSSRTTQPLGKAI 218
           LYTH+   H   + IN F      ++ +    K L+  E     LF +     +P G  +
Sbjct: 153 LYTHYRITHMEINQINTFICDIPLSVRMNISKKILIRTEHLTNHLFAVQC-FREPYGVYV 212

Query: 219 TVHSIGFASSKEEEEEGLSLELVLKTKETTLRLQSVMKGIKELVDEMPPEEFLVIPN 274
           TV  I   SS E  +   +L   +    T +     +K + +L  + P E F++IPN
Sbjct: 213 TVSCIA-PSSPELSQYSYALSYTV-DGHTVIYQSPEVKRVLKLSFQTPQENFMLIPN 266

[Close]

BLAST of Lsi05G002880 vs. TAIR10
Match: AT1G66660.2 (AT1G66660.2 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 145.6 bits (366), Expect = 4.6e-35
Identity = 97/279 (34.77%), Postives = 150/279 (53.76%), Query Frame = 1


Query: 7   QNDQDAQPEQAEEENPAVNTPEPETNLKT---SGKKRRIESQNVTLIDPEILDCYICCEP 66
           +++++ + E+ E E       E E N+ T   SG  +   SQ V L   ++LDC  CCEP
Sbjct: 55  EDEEEFEEEEEELEEEEDEEEEEEENVTTDEQSGSPK--SSQPVKLQSSDVLDCPTCCEP 114

Query: 67  LAIPVFQCENGHVACSDCCSKNVEECPFCHKKIGDIRCRAIETVLESLKGACQNSKYGCK 126
           L  P++QC NGH+ACS CC K  ++C FC   IGDIRCRA+E V+E+    C N+K+GCK
Sbjct: 115 LKRPIYQCSNGHLACSSCCQKLNKKCSFCRCNIGDIRCRAMEKVIEASIVPCPNAKHGCK 174

Query: 127 AKPTFDHRKEHWNKCSFSPCRCPISNCDYVGPSKKLYTH-FNQKHPSSAINFHYY----- 186
              T+ ++  H   C F  C CP+SNC+YV     L +H  +  H     + H+      
Sbjct: 175 ETTTYCNQSSHEKVCKFVRCSCPVSNCNYVSSYSNLKSHACSTAHVWGEDDIHFQLVIDR 234

Query: 187 -SSFTINLKQDDKFLVLREKRAGVLFVLSSRTTQPLGKAITVHSIGFASSKEEEEEGLSL 246
              F +NL +  K +V +E++ G L V+ +      G  +TV+ I   +    +   LS 
Sbjct: 235 PRIFNMNLGR-KKTVVFKEEKEGDLIVVQAFKGLE-GVYVTVNRIAHMAPGIRD---LSC 294

Query: 247 ELVLKTKETTLRLQSVMKGIKELVDEMPPEEFLV-IPNK 275
            L    + +TLR  S++K I+++ ++M  E+ L+ IP K
Sbjct: 295 SLAKLNEYSTLRSGSLVKKIQKVREKMHLEDDLMWIPPK 326

[Close]

BLAST of Lsi05G002880 vs. NCBI nr
Match: gi|643706681|gb|KDP22664.1| (hypothetical protein JCGZ_02506 [Jatropha curcas])

HSP 1 Score: 216.9 bits (551), Expect = 4.6e-53
Identity = 117/270 (43.33%), Postives = 163/270 (60.37%), Query Frame = 1


Query: 8   NDQDAQPEQAEEENPAVNTPEPETNLKTSGKKRRIESQNVTLIDPEILDCYICCEPLAIP 67
           +D D   EQ EE    V      +N++      R     VTL DPE+LDC +C E L IP
Sbjct: 68  DDDDDDEEQEEETTEVVTNQIAMSNVRAPIGPTRNGGICVTLTDPEVLDCPVCYESLTIP 127

Query: 68  VFQCENGHVACSDCCSKNVEECPFCHKKIGDIRCRAIETVLESLKGACQNSKYGCKAKPT 127
           VFQCENGH ACS CC K +++CP C   IG  RCRAIE VLES++ +CQN KYGCK    
Sbjct: 128 VFQCENGHTACSSCCKKLLQKCPTCSLPIGYNRCRAIEKVLESVRLSCQNLKYGCKEIVN 187

Query: 128 FDHRKEHWNKCSFSPCRCPISNCDYVGPSKKLYTHFNQKHPSSAINFHYYSSFTINLKQD 187
           +  + +H   CS +PC CP S C +VG S+++Y HF+ KH  +A+ F Y ++F +    +
Sbjct: 188 YSKKVDHEKICSHAPCFCPSSGCSFVGSSRQIYQHFSNKHKGAAVLFRYGNTFPVFFTLN 247

Query: 188 DKFLVLREKRAGVLFVLSSRTTQPLGKAITVHSIGFASSKEEEEEGLSLELVLKTKETTL 247
           DK  +LRE++ GVLF+L++ T + +G  ITV  IG +S K     G   EL  K + + L
Sbjct: 248 DKSRILREEKEGVLFILNN-TAEVVGNIITVSCIGPSSPK----GGYFYELAAKMEGSNL 307

Query: 248 RLQSVMKGIKELVDEMP-PEEFLVIPNKFF 277
           + QS  K I+++ D+ P  ++FLV+P  FF
Sbjct: 308 KFQSFTKNIRKVNDDDPHSQDFLVVPASFF 332

[Close]

BLAST of Lsi05G002880 vs. NCBI nr
Match: gi|802762064|ref|XP_012089939.1| (PREDICTED: E3 ubiquitin-protein ligase SINA-like 10 [Jatropha curcas])

HSP 1 Score: 216.9 bits (551), Expect = 4.6e-53
Identity = 117/270 (43.33%), Postives = 163/270 (60.37%), Query Frame = 1


Query: 8   NDQDAQPEQAEEENPAVNTPEPETNLKTSGKKRRIESQNVTLIDPEILDCYICCEPLAIP 67
           +D D   EQ EE    V      +N++      R     VTL DPE+LDC +C E L IP
Sbjct: 68  DDDDDDEEQEEETTEVVTNQIAMSNVRAPIGPTRNGGICVTLTDPEVLDCPVCYESLTIP 127

Query: 68  VFQCENGHVACSDCCSKNVEECPFCHKKIGDIRCRAIETVLESLKGACQNSKYGCKAKPT 127
           VFQCENGH ACS CC K +++CP C   IG  RCRAIE VLES++ +CQN KYGCK    
Sbjct: 128 VFQCENGHTACSSCCKKLLQKCPTCSLPIGYNRCRAIEKVLESVRLSCQNLKYGCKEIVN 187

Query: 128 FDHRKEHWNKCSFSPCRCPISNCDYVGPSKKLYTHFNQKHPSSAINFHYYSSFTINLKQD 187
           +  + +H   CS +PC CP S C +VG S+++Y HF+ KH  +A+ F Y ++F +    +
Sbjct: 188 YSKKVDHEKICSHAPCFCPSSGCSFVGSSRQIYQHFSNKHKGAAVLFRYGNTFPVFFTLN 247

Query: 188 DKFLVLREKRAGVLFVLSSRTTQPLGKAITVHSIGFASSKEEEEEGLSLELVLKTKETTL 247
           DK  +LRE++ GVLF+L++ T + +G  ITV  IG +S K     G   EL  K + + L
Sbjct: 248 DKSRILREEKEGVLFILNN-TAEVVGNIITVSCIGPSSPK----GGYFYELAAKMEGSNL 307

Query: 248 RLQSVMKGIKELVDEMP-PEEFLVIPNKFF 277
           + QS  K I+++ D+ P  ++FLV+P  FF
Sbjct: 308 KFQSFTKNIRKVNDDDPHSQDFLVVPASFF 332

[Close]

BLAST of Lsi05G002880 vs. NCBI nr
Match: gi|778679778|ref|XP_004140738.2| (PREDICTED: E3 ubiquitin-protein ligase SINA-like 10 [Cucumis sativus])

HSP 1 Score: 215.7 bits (548), Expect = 1.0e-52
Identity = 111/231 (48.05%), Postives = 146/231 (63.20%), Query Frame = 1


Query: 46  NVTLIDPEILDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKKIGDIRCRAIE 105
           NV   DP+ILDCYICCEPL+IPVFQCENGH+ACS CC+K   +CP C   IG IRCRAIE
Sbjct: 28  NVIFTDPQILDCYICCEPLSIPVFQCENGHIACSSCCTKAQNKCPSCTLAIGYIRCRAIE 87

Query: 106 TVLESLKGACQNSKYGCKAKPTFDHRKEHWNKCSFSPCRCPISNCDYVGPSKKLYTHFNQ 165
            VLES+K  CQN+ YGCK     +   +H + C + PC CP+ NC +VG +++L  HF +
Sbjct: 88  KVLESIKLPCQNAIYGCKTVMGLNLINDHESLCRYEPCSCPLDNCTFVGSTEQLGLHFTK 147

Query: 166 KHPSSAINFHYYSSFTINLKQDDKFLVLREKRAGVLFVLSSRTTQPLGKAITVHSIGFAS 225
           KH  SA  F Y + FTI L   D   +L+ +  GVLF L S T +  G A+T++ IG  S
Sbjct: 148 KHKDSAKIFSYNTRFTICLNNGDTHRILKAENDGVLFFL-SYTFEIFGNAVTMNRIGPLS 207

Query: 226 SKEEEEEGLSLELVLKTKETTLRLQSVMKGIKELVDEMPPEEFLVIPNKFF 277
           S    E+    E+  KT  + L LQS+ K I+ L+   P +  L+IPN++F
Sbjct: 208 S----EKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVPPSKGSLLIPNEYF 253

[Close]

BLAST of Lsi05G002880 vs. NCBI nr
Match: gi|566210590|ref|XP_006372377.1| (seven in absentia family protein [Populus trichocarpa])

HSP 1 Score: 214.5 bits (545), Expect = 2.3e-52
Identity = 109/228 (47.81%), Postives = 142/228 (62.28%), Query Frame = 1


Query: 48  TLIDPEILDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKKIGDIRCRAIETV 107
           TL DPE+LDC ICCEPL IPVFQC+NGH ACS CC K   +CP C   IG  RCRAIE V
Sbjct: 148 TLSDPEVLDCPICCEPLTIPVFQCDNGHTACSSCCIKLQHKCPSCTMPIGYNRCRAIEKV 207

Query: 108 LESLKGACQNSKYGCKAKPTFDHRKEHWNKCSFSPCRCPISNCDYVGPSKKLYTHFNQKH 167
           LESLK +C NS YGCK    +  + EH   C+ +PC CP+  C+Y G SK+LY H   KH
Sbjct: 208 LESLKVSCSNSSYGCKESICYSKKYEHDKSCTHAPCTCPLPACNYQGSSKRLYQHCRIKH 267

Query: 168 PSSAINFHYYSSFTINLKQDDKFLVLREKRAGVLFVLSSRTTQPLGKAITVHSIGFASSK 227
                +F + +SF +    D KF VL+E++  VLF+L++R ++ LG  ITV  +G +SSK
Sbjct: 268 LCDLTSFQFNTSFPLFFMVDHKFRVLQEEKEDVLFILTNR-SECLGNVITVSCMGPSSSK 327

Query: 228 EEEEEGLSLELVLKTKETTLRLQSVMKGIKELVDEMPPEEFLVIPNKF 276
               +G   EL  K + + +R QS  + I+  VD  P   FL++PN F
Sbjct: 328 ----QGYFYELTAKAEGSNVRFQSSTRNIQTRVDHPPSLGFLLVPNDF 370

[Close]

BLAST of Lsi05G002880 vs. NCBI nr
Match: gi|743905282|ref|XP_011046039.1| (PREDICTED: E3 ubiquitin-protein ligase SINA-like 7 [Populus euphratica])

HSP 1 Score: 213.8 bits (543), Expect = 3.9e-52
Identity = 109/228 (47.81%), Postives = 140/228 (61.40%), Query Frame = 1


Query: 48  TLIDPEILDCYICCEPLAIPVFQCENGHVACSDCCSKNVEECPFCHKKIGDIRCRAIETV 107
           TL DPE+LDC ICCE L IPVFQCENGH ACS CC K   +CP C   IG  RCRAIE V
Sbjct: 149 TLSDPEVLDCPICCESLTIPVFQCENGHTACSSCCRKLQHKCPSCAMPIGYNRCRAIEKV 208

Query: 108 LESLKGACQNSKYGCKAKPTFDHRKEHWNKCSFSPCRCPISNCDYVGPSKKLYTHFNQKH 167
           LESLK +C N  YGC+    +  + EH   C+ +PC CP+  C+Y G SK LY H   KH
Sbjct: 209 LESLKVSCSNRSYGCRESICYSKKYEHDKSCAHAPCTCPLPACNYQGSSKHLYLHCRSKH 268

Query: 168 PSSAINFHYYSSFTINLKQDDKFLVLREKRAGVLFVLSSRTTQPLGKAITVHSIGFASSK 227
                +F + +SF +    D KFLVL+E++ GVLF+L++R ++ LG  ITV  +G +SSK
Sbjct: 269 LCDLTSFQFNTSFPLFFMVDHKFLVLQEEKEGVLFILTNR-SECLGNVITVSCMGPSSSK 328

Query: 228 EEEEEGLSLELVLKTKETTLRLQSVMKGIKELVDEMPPEEFLVIPNKF 276
               +G   EL  K   + +R QS  + I+  VD  P   FL++PN F
Sbjct: 329 ----QGYFYELTAKAVGSNVRFQSSTRNIQTRVDHPPSLGFLLVPNDF 371

The following BLAST results are available for this feature:

BLAST of Lsi05G002880 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SIL10_ARATH	4.5e-40	34.22	E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana GN=At5g37930 PE...	[more]
SINL4_ARATH	6.7e-36	33.93	E3 ubiquitin-protein ligase SINA-like 4 OS=Arabidopsis thaliana GN=At1g66650 PE=...	[more]
SINL6_ARATH	4.3e-35	37.76	Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana GN=At5g...	[more]
SINL7_ARATH	1.3e-34	36.29	E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana GN=At5g37890 PE=...	[more]
SINL5_ARATH	8.2e-34	34.77	E3 ubiquitin-protein ligase SINA-like 5 OS=Arabidopsis thaliana GN=At1g66660 PE=...	[more]

BLAST of Lsi05G002880 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A067JT13_JATCU	3.2e-53	43.33	E3 ubiquitin-protein ligase OS=Jatropha curcas GN=JCGZ_02506 PE=3 SV=1	[more]
A0A0A0L9Z6_CUCSA	7.1e-53	48.05	E3 ubiquitin-protein ligase OS=Cucumis sativus GN=Csa_3G185660 PE=3 SV=1	[more]
U5FIU4_POPTR	1.6e-52	47.81	E3 ubiquitin-protein ligase OS=Populus trichocarpa GN=POPTR_0017s01040g PE=3 SV=...	[more]
A0A061EGM7_THECC	1.0e-51	40.88	E3 ubiquitin-protein ligase OS=Theobroma cacao GN=TCM_019286 PE=3 SV=1	[more]
A0A061EG86_THECC	1.0e-51	40.88	E3 ubiquitin-protein ligase OS=Theobroma cacao GN=TCM_019286 PE=3 SV=1	[more]

BLAST of Lsi05G002880 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G37930.1	2.5e-41	34.22	Protein with RING/U-box and TRAF-like domains	[more]
AT1G66650.1	3.8e-37	33.93	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37870.1	2.4e-36	37.76	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37890.1	7.1e-36	36.29	Protein with RING/U-box and TRAF-like domains	[more]
AT1G66660.2	4.6e-35	34.77	Protein with RING/U-box and TRAF-like domains	[more]

BLAST of Lsi05G002880 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|643706681\|gb\|KDP22664.1\|	4.6e-53	43.33	hypothetical protein JCGZ_02506 [Jatropha curcas]	[more]
gi\|802762064\|ref\|XP_012089939.1\|	4.6e-53	43.33	PREDICTED: E3 ubiquitin-protein ligase SINA-like 10 [Jatropha curcas]	[more]
gi\|778679778\|ref\|XP_004140738.2\|	1.0e-52	48.05	PREDICTED: E3 ubiquitin-protein ligase SINA-like 10 [Cucumis sativus]	[more]
gi\|566210590\|ref\|XP_006372377.1\|	2.3e-52	47.81	seven in absentia family protein [Populus trichocarpa]	[more]
gi\|743905282\|ref\|XP_011046039.1\|	3.9e-52	47.81	PREDICTED: E3 ubiquitin-protein ligase SINA-like 7 [Populus euphratica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0016567	protein ubiquitination
GO:0007275	multicellular organism development
GO:0006511	ubiquitin-dependent protein catabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0004842	ubiquitin-protein transferase activity
GO:0008270	zinc ion binding
GO:0005515	protein binding

Vocabulary: **Cellular Component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **INTERPRO**
Term	Definition
IPR018121	7-in-absentia-prot_TRAF-dom
IPR013323	SIAH-type domain
IPR013083	Znf_RING/FYVE/PHD
IPR013010	Znf_SIAH
IPR008974	TRAF-like
IPR004162	SINA-like

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007275	multicellular organism development
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0016874	ligase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G002880.1	Lsi05G002880.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004162	E3 ubiquitin-protein ligase SIN-like	PANTHER	PTHR10315	SEVEN IN ABSENTIA HOMOLOG	coord: 8..276 score: 1.7
IPR008974	TRAF-like	unknown	SSF49599	TRAF domain-like	coord: 111..276 score: 3.99
IPR013010	Zinc finger, SIAH-type	PROFILE	PS51081	ZF_SIAH	coord: 110..168 score: 12
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 53..117 score: 2.
IPR013323	SIAH-type domain	GENE3D	G3DSA:3.90.890.10		coord: 118..163 score: 3.1
IPR018121	Seven-in-absentia protein, TRAF-like domain	PFAM	PF03145	Sina	coord: 99..263 score: 8.9
None	No IPR available	PANTHER	PTHR10315:SF43	E3 UBIQUITIN-PROTEIN LIGASE	coord: 8..276 score: 1.7

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G002880	Csa3G185660	Cucumber (Chinese Long) v2	culsiB211
Lsi05G002880	CSPI03G17530	Wild cucumber (PI 183967)	cpilsiB209
Lsi05G002880	Cucsa.108380	Cucumber (Gy14) v1	cgylsiB144
Lsi05G002880	CmaCh01G003460	Cucurbita maxima (Rimu)	cmalsiB440
Lsi05G002880	CmoCh01G003640	Cucurbita moschata (Rifu)	cmolsiB430
Lsi05G002880	Cla013155	Watermelon (97103) v1	lsiwmB361
Lsi05G002880	ClCG05G010790	Watermelon (Charleston Gray)	lsiwcgB332
Lsi05G002880	MELO3C020308.2	Melon (DHL92) v3.6.1	lsimedB384
Lsi05G002880	Cla97C05G093110	Watermelon (97103) v2	lsiwmbB329
Lsi05G002880	Bhi01G001334	Wax gourd	lsiwgoB431
Lsi05G002880	Cp4.1LG02g16790	Cucurbita pepo (Zucchini)	cpelsiB470
Lsi05G002880	CsGy3G017560	Cucumber (Gy14) v2	cgyblsiB198
Lsi05G002880	CsaV3_3G017700	Cucumber (Chinese Long) v3	cuclsiB230

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G002880	Cucumber (Gy14) v1	cgylsiB322
Lsi05G002880	Cucurbita maxima (Rimu)	cmalsiB249
Lsi05G002880	Cucurbita moschata (Rifu)	cmolsiB237
Lsi05G002880	Cucurbita pepo (Zucchini)	cpelsiB511
Lsi05G002880	Melon (DHL92) v3.5.1	lsimeB368
Lsi05G002880	Cucumber (Gy14) v2	cgyblsiB199
Lsi05G002880	Silver-seed gourd	carlsiB158
Lsi05G002880	Silver-seed gourd	carlsiB700
Lsi05G002880	Cucumber (Chinese Long) v3	cuclsiB231